superscript iii first strand synthesis system Thermo Fisher Search Results


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  • 79
    Thermo Fisher superscript iii first strand synthesis ssiii supermix
    Superscript Iii First Strand Synthesis Ssiii Supermix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript iii ssiii first strand synthesis system
    Superscript Iii Ssiii First Strand Synthesis System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript iii
    Superscript Iii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 40954 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript iii first strand synthesis
    Superscript Iii First Strand Synthesis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1151 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript iii first strand kit
    SDS3 promotes CD8 + T cell activation and T cell infiltration. (A) Flow cytometry analysis of early immune changes within the lungs of MMTV-PyMT mice i.v. injected with 1 × 10 5 VO-PyMT-GFP-Luc cells at 7 wk of age treated with SDS3 or IgG isotype control. Counting beads used to normalize frequencies with absolute cell counts standardized to isotype control expressed as a relative difference from the average isotype control absolute cell count. Significant activation of T cells seen through marked elevation of ICOS and CD25 (n = 3 IgG, n = 4 SDS3). (B) Flow cytometry analysis of ICOS-activated T cells reveals both CD4 and CD8 T cell subsets significantly up-regulated after SDS3 treatment. (C) Flow cytometry analysis of IFNγ within CD4- and CD8-activated T cells (ICOS+) demonstrates prominent Th1 cytokine skewing of CD8 T cells after SDS3 treatment. (D) Representative scatterplot of ICOS+ CD8 T-cell expression of IFNγ. Red box represents IgG-treated activated CD8 T cells expressing IFNγ. Green box represents SDS3-treated activated CD8 T cells expressing IFNγ. (E) Flow cytometry analysis of PD-1 within CD8 + -activated T cells (ICOS+) shows significant up-regulation of an exhausted T-cell response due to overactivation of the immune system. (F) Representative scatterplot of ICOS+ CD8 T-cell expression of PD-1. Red box represents IgG-treated activated CD8 T cells expressing PD-1. Green box represents SDS3-treated activated CD8 T cells expressing PD-1. (G ) <t>qPCR</t> analysis of MMTV-PyMT lungs i.v. injected with 1 × 10 5 VO-PyMT-GFP-Luc cells 1 wk after IgG or SDS3 treatment shows decreased Th2/M2-like markers ( Il-4r and Egr2 ) and increased Il-12 (duplicate conditions with n = 4 IgG and n = 4 SDS3). (H) Left: representative immunofluorescence images of 9-wk-old MMTV-PyMT lungs i.v. injected with 1 × 10 5 VO-PyMT-GFP-Luc cells 3 wk after IgG or SDS3 treatment. CD8 + T cells localize around GFP + metastatic sites (arrows) after IgG and SDS3 treatment, whereas lower presence of CD8 + T cells seen around GFP − sites. Middle: quantification of CD8 + T cells in GFP + and GFP − regions 3 wk after IgG and SDS3 treatment (not shown: GFP − foci). Right: CD8 + T cell infiltration around periphery and within GFP + metastatic foci in MMTV-PyMT mice at 9-wk of age after a 3-wk chase post i.v. injection of 1 × 10 5 VO-PyMT GFP + cells (n = 5 IgG, n = 5 SDS3; <t>three</t> serial sections used for quantification).
    Superscript Iii First Strand Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2236 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript iii first strand synthesis supermix
    SDS3 promotes CD8 + T cell activation and T cell infiltration. (A) Flow cytometry analysis of early immune changes within the lungs of MMTV-PyMT mice i.v. injected with 1 × 10 5 VO-PyMT-GFP-Luc cells at 7 wk of age treated with SDS3 or IgG isotype control. Counting beads used to normalize frequencies with absolute cell counts standardized to isotype control expressed as a relative difference from the average isotype control absolute cell count. Significant activation of T cells seen through marked elevation of ICOS and CD25 (n = 3 IgG, n = 4 SDS3). (B) Flow cytometry analysis of ICOS-activated T cells reveals both CD4 and CD8 T cell subsets significantly up-regulated after SDS3 treatment. (C) Flow cytometry analysis of IFNγ within CD4- and CD8-activated T cells (ICOS+) demonstrates prominent Th1 cytokine skewing of CD8 T cells after SDS3 treatment. (D) Representative scatterplot of ICOS+ CD8 T-cell expression of IFNγ. Red box represents IgG-treated activated CD8 T cells expressing IFNγ. Green box represents SDS3-treated activated CD8 T cells expressing IFNγ. (E) Flow cytometry analysis of PD-1 within CD8 + -activated T cells (ICOS+) shows significant up-regulation of an exhausted T-cell response due to overactivation of the immune system. (F) Representative scatterplot of ICOS+ CD8 T-cell expression of PD-1. Red box represents IgG-treated activated CD8 T cells expressing PD-1. Green box represents SDS3-treated activated CD8 T cells expressing PD-1. (G ) <t>qPCR</t> analysis of MMTV-PyMT lungs i.v. injected with 1 × 10 5 VO-PyMT-GFP-Luc cells 1 wk after IgG or SDS3 treatment shows decreased Th2/M2-like markers ( Il-4r and Egr2 ) and increased Il-12 (duplicate conditions with n = 4 IgG and n = 4 SDS3). (H) Left: representative immunofluorescence images of 9-wk-old MMTV-PyMT lungs i.v. injected with 1 × 10 5 VO-PyMT-GFP-Luc cells 3 wk after IgG or SDS3 treatment. CD8 + T cells localize around GFP + metastatic sites (arrows) after IgG and SDS3 treatment, whereas lower presence of CD8 + T cells seen around GFP − sites. Middle: quantification of CD8 + T cells in GFP + and GFP − regions 3 wk after IgG and SDS3 treatment (not shown: GFP − foci). Right: CD8 + T cell infiltration around periphery and within GFP + metastatic foci in MMTV-PyMT mice at 9-wk of age after a 3-wk chase post i.v. injection of 1 × 10 5 VO-PyMT GFP + cells (n = 5 IgG, n = 5 SDS3; <t>three</t> serial sections used for quantification).
    Superscript Iii First Strand Synthesis Supermix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8300 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript iii first strand synthesis system
    Inhibition of 5637 cell proliferation and receptor activation by <t>anti-HER2</t> and anti-EGFR agents. Cells were treated with HER2 or EGFR inhibitors for 96 h. ( a ) Cell viability was measured by WST-1 kit as described in materials and methods. Relative cell viability as a percent was determined as ((absorbance in each treatment set—absorbance in untreated set)/absorbance in non-treated set X 100). Results represent the mean ± SD obtained from <t>three</t> independent experiments. * p
    Superscript Iii First Strand Synthesis System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 38609 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript iii first strand synthesis kit
    Inhibition of 5637 cell proliferation and receptor activation by <t>anti-HER2</t> and anti-EGFR agents. Cells were treated with HER2 or EGFR inhibitors for 96 h. ( a ) Cell viability was measured by WST-1 kit as described in materials and methods. Relative cell viability as a percent was determined as ((absorbance in each treatment set—absorbance in untreated set)/absorbance in non-treated set X 100). Results represent the mean ± SD obtained from <t>three</t> independent experiments. * p
    Superscript Iii First Strand Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7709 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript iii first dna kit
    Inhibition of 5637 cell proliferation and receptor activation by <t>anti-HER2</t> and anti-EGFR agents. Cells were treated with HER2 or EGFR inhibitors for 96 h. ( a ) Cell viability was measured by WST-1 kit as described in materials and methods. Relative cell viability as a percent was determined as ((absorbance in each treatment set—absorbance in untreated set)/absorbance in non-treated set X 100). Results represent the mean ± SD obtained from <t>three</t> independent experiments. * p
    Superscript Iii First Dna Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript iii cdna synthesis kit
    Inhibition of 5637 cell proliferation and receptor activation by <t>anti-HER2</t> and anti-EGFR agents. Cells were treated with HER2 or EGFR inhibitors for 96 h. ( a ) Cell viability was measured by WST-1 kit as described in materials and methods. Relative cell viability as a percent was determined as ((absorbance in each treatment set—absorbance in untreated set)/absorbance in non-treated set X 100). Results represent the mean ± SD obtained from <t>three</t> independent experiments. * p
    Superscript Iii Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1930 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript iii first strand synthesis supermix assay
    Inhibition of 5637 cell proliferation and receptor activation by <t>anti-HER2</t> and anti-EGFR agents. Cells were treated with HER2 or EGFR inhibitors for 96 h. ( a ) Cell viability was measured by WST-1 kit as described in materials and methods. Relative cell viability as a percent was determined as ((absorbance in each treatment set—absorbance in untreated set)/absorbance in non-treated set X 100). Results represent the mean ± SD obtained from <t>three</t> independent experiments. * p
    Superscript Iii First Strand Synthesis Supermix Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript iii first strand cdna synthesis
    Inhibition of 5637 cell proliferation and receptor activation by <t>anti-HER2</t> and anti-EGFR agents. Cells were treated with HER2 or EGFR inhibitors for 96 h. ( a ) Cell viability was measured by WST-1 kit as described in materials and methods. Relative cell viability as a percent was determined as ((absorbance in each treatment set—absorbance in untreated set)/absorbance in non-treated set X 100). Results represent the mean ± SD obtained from <t>three</t> independent experiments. * p
    Superscript Iii First Strand Cdna Synthesis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 141 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript iii first strand synthesis supermix kit
    Inhibition of 5637 cell proliferation and receptor activation by <t>anti-HER2</t> and anti-EGFR agents. Cells were treated with HER2 or EGFR inhibitors for 96 h. ( a ) Cell viability was measured by WST-1 kit as described in materials and methods. Relative cell viability as a percent was determined as ((absorbance in each treatment set—absorbance in untreated set)/absorbance in non-treated set X 100). Results represent the mean ± SD obtained from <t>three</t> independent experiments. * p
    Superscript Iii First Strand Synthesis Supermix Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1299 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript iii first strand synthesis system kit
    Inhibition of 5637 cell proliferation and receptor activation by <t>anti-HER2</t> and anti-EGFR agents. Cells were treated with HER2 or EGFR inhibitors for 96 h. ( a ) Cell viability was measured by WST-1 kit as described in materials and methods. Relative cell viability as a percent was determined as ((absorbance in each treatment set—absorbance in untreated set)/absorbance in non-treated set X 100). Results represent the mean ± SD obtained from <t>three</t> independent experiments. * p
    Superscript Iii First Strand Synthesis System Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 770 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript iii one step rt pcr system
    Inhibition of 5637 cell proliferation and receptor activation by <t>anti-HER2</t> and anti-EGFR agents. Cells were treated with HER2 or EGFR inhibitors for 96 h. ( a ) Cell viability was measured by WST-1 kit as described in materials and methods. Relative cell viability as a percent was determined as ((absorbance in each treatment set—absorbance in untreated set)/absorbance in non-treated set X 100). Results represent the mean ± SD obtained from <t>three</t> independent experiments. * p
    Superscript Iii One Step Rt Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6305 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript iii first strand cdna synthesis kit
    Inhibition of 5637 cell proliferation and receptor activation by <t>anti-HER2</t> and anti-EGFR agents. Cells were treated with HER2 or EGFR inhibitors for 96 h. ( a ) Cell viability was measured by WST-1 kit as described in materials and methods. Relative cell viability as a percent was determined as ((absorbance in each treatment set—absorbance in untreated set)/absorbance in non-treated set X 100). Results represent the mean ± SD obtained from <t>three</t> independent experiments. * p
    Superscript Iii First Strand Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2254 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript iii platinum first strand synthesis supermix
    Inhibition of 5637 cell proliferation and receptor activation by <t>anti-HER2</t> and anti-EGFR agents. Cells were treated with HER2 or EGFR inhibitors for 96 h. ( a ) Cell viability was measured by WST-1 kit as described in materials and methods. Relative cell viability as a percent was determined as ((absorbance in each treatment set—absorbance in untreated set)/absorbance in non-treated set X 100). Results represent the mean ± SD obtained from <t>three</t> independent experiments. * p
    Superscript Iii Platinum First Strand Synthesis Supermix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 70/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript iii first strand cdna synthesis system
    Inhibition of 5637 cell proliferation and receptor activation by <t>anti-HER2</t> and anti-EGFR agents. Cells were treated with HER2 or EGFR inhibitors for 96 h. ( a ) Cell viability was measured by WST-1 kit as described in materials and methods. Relative cell viability as a percent was determined as ((absorbance in each treatment set—absorbance in untreated set)/absorbance in non-treated set X 100). Results represent the mean ± SD obtained from <t>three</t> independent experiments. * p
    Superscript Iii First Strand Cdna Synthesis System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 621 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher platinum superscript iii first strand synthesis system
    Inhibition of 5637 cell proliferation and receptor activation by <t>anti-HER2</t> and anti-EGFR agents. Cells were treated with HER2 or EGFR inhibitors for 96 h. ( a ) Cell viability was measured by WST-1 kit as described in materials and methods. Relative cell viability as a percent was determined as ((absorbance in each treatment set—absorbance in untreated set)/absorbance in non-treated set X 100). Results represent the mean ± SD obtained from <t>three</t> independent experiments. * p
    Platinum Superscript Iii First Strand Synthesis System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 70/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript iii first strand rt pcr kit
    Inhibition of 5637 cell proliferation and receptor activation by <t>anti-HER2</t> and anti-EGFR agents. Cells were treated with HER2 or EGFR inhibitors for 96 h. ( a ) Cell viability was measured by WST-1 kit as described in materials and methods. Relative cell viability as a percent was determined as ((absorbance in each treatment set—absorbance in untreated set)/absorbance in non-treated set X 100). Results represent the mean ± SD obtained from <t>three</t> independent experiments. * p
    Superscript Iii First Strand Rt Pcr Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 178 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript iii rts first strand cdna synthesis kit
    Inhibition of 5637 cell proliferation and receptor activation by <t>anti-HER2</t> and anti-EGFR agents. Cells were treated with HER2 or EGFR inhibitors for 96 h. ( a ) Cell viability was measured by WST-1 kit as described in materials and methods. Relative cell viability as a percent was determined as ((absorbance in each treatment set—absorbance in untreated set)/absorbance in non-treated set X 100). Results represent the mean ± SD obtained from <t>three</t> independent experiments. * p
    Superscript Iii Rts First Strand Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript iii vilo master mix
    Inhibition of 5637 cell proliferation and receptor activation by <t>anti-HER2</t> and anti-EGFR agents. Cells were treated with HER2 or EGFR inhibitors for 96 h. ( a ) Cell viability was measured by WST-1 kit as described in materials and methods. Relative cell viability as a percent was determined as ((absorbance in each treatment set—absorbance in untreated set)/absorbance in non-treated set X 100). Results represent the mean ± SD obtained from <t>three</t> independent experiments. * p
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    Thermo Fisher superscript iii reverse transcriptase
    Inhibition of 5637 cell proliferation and receptor activation by <t>anti-HER2</t> and anti-EGFR agents. Cells were treated with HER2 or EGFR inhibitors for 96 h. ( a ) Cell viability was measured by WST-1 kit as described in materials and methods. Relative cell viability as a percent was determined as ((absorbance in each treatment set—absorbance in untreated set)/absorbance in non-treated set X 100). Results represent the mean ± SD obtained from <t>three</t> independent experiments. * p
    Superscript Iii Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 66567 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript iii kit
    Inhibition of 5637 cell proliferation and receptor activation by <t>anti-HER2</t> and anti-EGFR agents. Cells were treated with HER2 or EGFR inhibitors for 96 h. ( a ) Cell viability was measured by WST-1 kit as described in materials and methods. Relative cell viability as a percent was determined as ((absorbance in each treatment set—absorbance in untreated set)/absorbance in non-treated set X 100). Results represent the mean ± SD obtained from <t>three</t> independent experiments. * p
    Superscript Iii Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7833 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rna 6000 nano kit superscript iii first strand synthesis supermix kit
    Inhibition of 5637 cell proliferation and receptor activation by <t>anti-HER2</t> and anti-EGFR agents. Cells were treated with HER2 or EGFR inhibitors for 96 h. ( a ) Cell viability was measured by WST-1 kit as described in materials and methods. Relative cell viability as a percent was determined as ((absorbance in each treatment set—absorbance in untreated set)/absorbance in non-treated set X 100). Results represent the mean ± SD obtained from <t>three</t> independent experiments. * p
    Rna 6000 Nano Kit Superscript Iii First Strand Synthesis Supermix Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher supersript iii first strand synthesis system
    Inhibition of 5637 cell proliferation and receptor activation by <t>anti-HER2</t> and anti-EGFR agents. Cells were treated with HER2 or EGFR inhibitors for 96 h. ( a ) Cell viability was measured by WST-1 kit as described in materials and methods. Relative cell viability as a percent was determined as ((absorbance in each treatment set—absorbance in untreated set)/absorbance in non-treated set X 100). Results represent the mean ± SD obtained from <t>three</t> independent experiments. * p
    Supersript Iii First Strand Synthesis System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 70/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscipt iii first strand synthesis kit
    Inhibition of 5637 cell proliferation and receptor activation by <t>anti-HER2</t> and anti-EGFR agents. Cells were treated with HER2 or EGFR inhibitors for 96 h. ( a ) Cell viability was measured by WST-1 kit as described in materials and methods. Relative cell viability as a percent was determined as ((absorbance in each treatment set—absorbance in untreated set)/absorbance in non-treated set X 100). Results represent the mean ± SD obtained from <t>three</t> independent experiments. * p
    Superscipt Iii First Strand Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Inhibition of 5637 cell proliferation and receptor activation by <t>anti-HER2</t> and anti-EGFR agents. Cells were treated with HER2 or EGFR inhibitors for 96 h. ( a ) Cell viability was measured by WST-1 kit as described in materials and methods. Relative cell viability as a percent was determined as ((absorbance in each treatment set—absorbance in untreated set)/absorbance in non-treated set X 100). Results represent the mean ± SD obtained from <t>three</t> independent experiments. * p
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    Image Search Results


    SDS3 promotes CD8 + T cell activation and T cell infiltration. (A) Flow cytometry analysis of early immune changes within the lungs of MMTV-PyMT mice i.v. injected with 1 × 10 5 VO-PyMT-GFP-Luc cells at 7 wk of age treated with SDS3 or IgG isotype control. Counting beads used to normalize frequencies with absolute cell counts standardized to isotype control expressed as a relative difference from the average isotype control absolute cell count. Significant activation of T cells seen through marked elevation of ICOS and CD25 (n = 3 IgG, n = 4 SDS3). (B) Flow cytometry analysis of ICOS-activated T cells reveals both CD4 and CD8 T cell subsets significantly up-regulated after SDS3 treatment. (C) Flow cytometry analysis of IFNγ within CD4- and CD8-activated T cells (ICOS+) demonstrates prominent Th1 cytokine skewing of CD8 T cells after SDS3 treatment. (D) Representative scatterplot of ICOS+ CD8 T-cell expression of IFNγ. Red box represents IgG-treated activated CD8 T cells expressing IFNγ. Green box represents SDS3-treated activated CD8 T cells expressing IFNγ. (E) Flow cytometry analysis of PD-1 within CD8 + -activated T cells (ICOS+) shows significant up-regulation of an exhausted T-cell response due to overactivation of the immune system. (F) Representative scatterplot of ICOS+ CD8 T-cell expression of PD-1. Red box represents IgG-treated activated CD8 T cells expressing PD-1. Green box represents SDS3-treated activated CD8 T cells expressing PD-1. (G ) qPCR analysis of MMTV-PyMT lungs i.v. injected with 1 × 10 5 VO-PyMT-GFP-Luc cells 1 wk after IgG or SDS3 treatment shows decreased Th2/M2-like markers ( Il-4r and Egr2 ) and increased Il-12 (duplicate conditions with n = 4 IgG and n = 4 SDS3). (H) Left: representative immunofluorescence images of 9-wk-old MMTV-PyMT lungs i.v. injected with 1 × 10 5 VO-PyMT-GFP-Luc cells 3 wk after IgG or SDS3 treatment. CD8 + T cells localize around GFP + metastatic sites (arrows) after IgG and SDS3 treatment, whereas lower presence of CD8 + T cells seen around GFP − sites. Middle: quantification of CD8 + T cells in GFP + and GFP − regions 3 wk after IgG and SDS3 treatment (not shown: GFP − foci). Right: CD8 + T cell infiltration around periphery and within GFP + metastatic foci in MMTV-PyMT mice at 9-wk of age after a 3-wk chase post i.v. injection of 1 × 10 5 VO-PyMT GFP + cells (n = 5 IgG, n = 5 SDS3; three serial sections used for quantification).

    Journal: Life Science Alliance

    Article Title: MMP9 modulates the metastatic cascade and immune landscape for breast cancer anti-metastatic therapy

    doi: 10.26508/lsa.201800226

    Figure Lengend Snippet: SDS3 promotes CD8 + T cell activation and T cell infiltration. (A) Flow cytometry analysis of early immune changes within the lungs of MMTV-PyMT mice i.v. injected with 1 × 10 5 VO-PyMT-GFP-Luc cells at 7 wk of age treated with SDS3 or IgG isotype control. Counting beads used to normalize frequencies with absolute cell counts standardized to isotype control expressed as a relative difference from the average isotype control absolute cell count. Significant activation of T cells seen through marked elevation of ICOS and CD25 (n = 3 IgG, n = 4 SDS3). (B) Flow cytometry analysis of ICOS-activated T cells reveals both CD4 and CD8 T cell subsets significantly up-regulated after SDS3 treatment. (C) Flow cytometry analysis of IFNγ within CD4- and CD8-activated T cells (ICOS+) demonstrates prominent Th1 cytokine skewing of CD8 T cells after SDS3 treatment. (D) Representative scatterplot of ICOS+ CD8 T-cell expression of IFNγ. Red box represents IgG-treated activated CD8 T cells expressing IFNγ. Green box represents SDS3-treated activated CD8 T cells expressing IFNγ. (E) Flow cytometry analysis of PD-1 within CD8 + -activated T cells (ICOS+) shows significant up-regulation of an exhausted T-cell response due to overactivation of the immune system. (F) Representative scatterplot of ICOS+ CD8 T-cell expression of PD-1. Red box represents IgG-treated activated CD8 T cells expressing PD-1. Green box represents SDS3-treated activated CD8 T cells expressing PD-1. (G ) qPCR analysis of MMTV-PyMT lungs i.v. injected with 1 × 10 5 VO-PyMT-GFP-Luc cells 1 wk after IgG or SDS3 treatment shows decreased Th2/M2-like markers ( Il-4r and Egr2 ) and increased Il-12 (duplicate conditions with n = 4 IgG and n = 4 SDS3). (H) Left: representative immunofluorescence images of 9-wk-old MMTV-PyMT lungs i.v. injected with 1 × 10 5 VO-PyMT-GFP-Luc cells 3 wk after IgG or SDS3 treatment. CD8 + T cells localize around GFP + metastatic sites (arrows) after IgG and SDS3 treatment, whereas lower presence of CD8 + T cells seen around GFP − sites. Middle: quantification of CD8 + T cells in GFP + and GFP − regions 3 wk after IgG and SDS3 treatment (not shown: GFP − foci). Right: CD8 + T cell infiltration around periphery and within GFP + metastatic foci in MMTV-PyMT mice at 9-wk of age after a 3-wk chase post i.v. injection of 1 × 10 5 VO-PyMT GFP + cells (n = 5 IgG, n = 5 SDS3; three serial sections used for quantification).

    Article Snippet: qPCR Total RNA was isolated from cells using the RNeasy Mini Kit (QIAGEN). cDNA was synthesized using the Superscript III RT First Strand Kit (Invitrogen). qPCR was performed using FastStart Universal SYBR Green master mix (Roche) in an Eppendorf Mastercycler Realplex machine.

    Techniques: Activation Assay, Flow Cytometry, Cytometry, Mouse Assay, Injection, Cell Counting, Expressing, Real-time Polymerase Chain Reaction, Immunofluorescence

    Inhibition of 5637 cell proliferation and receptor activation by anti-HER2 and anti-EGFR agents. Cells were treated with HER2 or EGFR inhibitors for 96 h. ( a ) Cell viability was measured by WST-1 kit as described in materials and methods. Relative cell viability as a percent was determined as ((absorbance in each treatment set—absorbance in untreated set)/absorbance in non-treated set X 100). Results represent the mean ± SD obtained from three independent experiments. * p

    Journal: Biomolecules

    Article Title: The HER2 S310F Mutant Can Form an Active Heterodimer with the EGFR, Which Can Be Inhibited by Cetuximab but Not by Trastuzumab as well as Pertuzumab

    doi: 10.3390/biom9100629

    Figure Lengend Snippet: Inhibition of 5637 cell proliferation and receptor activation by anti-HER2 and anti-EGFR agents. Cells were treated with HER2 or EGFR inhibitors for 96 h. ( a ) Cell viability was measured by WST-1 kit as described in materials and methods. Relative cell viability as a percent was determined as ((absorbance in each treatment set—absorbance in untreated set)/absorbance in non-treated set X 100). Results represent the mean ± SD obtained from three independent experiments. * p

    Article Snippet: After cDNA was synthesized using a SuperScript III First-Strand Synthesis system (Invitrogen), the HER2 gene fragment encoding from N302 residue to R340 residue was amplified using specific primer sets (HER2 forward: 5′-GCCTCCACTTCAACCACAGTGGC-3′ and HER2 reverse: 5′-CTGTGATCTCTTCCAGAGTCTCAAAC-3′).

    Techniques: Inhibition, Activation Assay