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    Thermo Fisher reverse transcriptase superscript ii
    ( a ) Results of qRT-PCR showing (i) Brn-3a and (ii) Brn-3b mRNA levels in the developing zebrafish at 24, 48 and 72 hpf. <t>cDNA</t> from total <t>RNA</t> was amplified using primers to ZF Brn-3a and Brn-3b and a variation in mRNA levels was corrected using ZF GAPDH. Values were expressed as fold induction relative to expression at 24 h (set at 1). ( b ) Representative western blot analysis showing single protein band for both Brn-3a and Brn-3b in extracts from adult ZF compared with adult mouse heart (M), used as a positive control. MW markers indicate the protein size and gamma ( γ ) tubulin was used to control for variation in total protein. ( c ) Representative images showing whole-mount immunostaining for (i) Brn-3a or (ii) Brn-3b (green; top panels) in ZF hearts at 72 hpf. Co-staining with tropomyosin (red; middle panels) indicate cardiomyocytes in the developing heart. Lower panel shows merge with bright field image. V, A, P (indicated by arrow). ( d ) Representative images of DAB-immunostained ZF embryos sections at 72 hpf. Protein localisation is seen as dark brown staining in ventricles (indicated by arrow), identified by TM, also shows Brn-3a and Brn-3b expression. × 40 magnification. A, atria; hpf, hours post fertilisation; MW, molecular weight; P, pericardial sac; TM, tropomyosin; V, ventricle; ZF, zebrafish heart
    Reverse Transcriptase Superscript Ii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8758 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcriptase superscript ii/product/Thermo Fisher
    Average 99 stars, based on 8758 article reviews
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    reverse transcriptase superscript ii - by Bioz Stars, 2020-07
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    84
    Thermo Fisher t9284 superscript ii reverse transcriptase thermo fisher scientific
    ( a ) Results of qRT-PCR showing (i) Brn-3a and (ii) Brn-3b mRNA levels in the developing zebrafish at 24, 48 and 72 hpf. <t>cDNA</t> from total <t>RNA</t> was amplified using primers to ZF Brn-3a and Brn-3b and a variation in mRNA levels was corrected using ZF GAPDH. Values were expressed as fold induction relative to expression at 24 h (set at 1). ( b ) Representative western blot analysis showing single protein band for both Brn-3a and Brn-3b in extracts from adult ZF compared with adult mouse heart (M), used as a positive control. MW markers indicate the protein size and gamma ( γ ) tubulin was used to control for variation in total protein. ( c ) Representative images showing whole-mount immunostaining for (i) Brn-3a or (ii) Brn-3b (green; top panels) in ZF hearts at 72 hpf. Co-staining with tropomyosin (red; middle panels) indicate cardiomyocytes in the developing heart. Lower panel shows merge with bright field image. V, A, P (indicated by arrow). ( d ) Representative images of DAB-immunostained ZF embryos sections at 72 hpf. Protein localisation is seen as dark brown staining in ventricles (indicated by arrow), identified by TM, also shows Brn-3a and Brn-3b expression. × 40 magnification. A, atria; hpf, hours post fertilisation; MW, molecular weight; P, pericardial sac; TM, tropomyosin; V, ventricle; ZF, zebrafish heart
    T9284 Superscript Ii Reverse Transcriptase Thermo Fisher Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t9284 superscript ii reverse transcriptase thermo fisher scientific/product/Thermo Fisher
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t9284 superscript ii reverse transcriptase thermo fisher scientific - by Bioz Stars, 2020-07
    84/100 stars
      Buy from Supplier

    99
    Thermo Fisher superscript ii reverse transcriptase thermo fisher scientific
    ( a ) Results of qRT-PCR showing (i) Brn-3a and (ii) Brn-3b mRNA levels in the developing zebrafish at 24, 48 and 72 hpf. <t>cDNA</t> from total <t>RNA</t> was amplified using primers to ZF Brn-3a and Brn-3b and a variation in mRNA levels was corrected using ZF GAPDH. Values were expressed as fold induction relative to expression at 24 h (set at 1). ( b ) Representative western blot analysis showing single protein band for both Brn-3a and Brn-3b in extracts from adult ZF compared with adult mouse heart (M), used as a positive control. MW markers indicate the protein size and gamma ( γ ) tubulin was used to control for variation in total protein. ( c ) Representative images showing whole-mount immunostaining for (i) Brn-3a or (ii) Brn-3b (green; top panels) in ZF hearts at 72 hpf. Co-staining with tropomyosin (red; middle panels) indicate cardiomyocytes in the developing heart. Lower panel shows merge with bright field image. V, A, P (indicated by arrow). ( d ) Representative images of DAB-immunostained ZF embryos sections at 72 hpf. Protein localisation is seen as dark brown staining in ventricles (indicated by arrow), identified by TM, also shows Brn-3a and Brn-3b expression. × 40 magnification. A, atria; hpf, hours post fertilisation; MW, molecular weight; P, pericardial sac; TM, tropomyosin; V, ventricle; ZF, zebrafish heart
    Superscript Ii Reverse Transcriptase Thermo Fisher Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript ii reverse transcriptase thermo fisher scientific/product/Thermo Fisher
    Average 99 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    superscript ii reverse transcriptase thermo fisher scientific - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    91
    Thermo Fisher reverse transcriptase
    ( a ) Results of qRT-PCR showing (i) Brn-3a and (ii) Brn-3b mRNA levels in the developing zebrafish at 24, 48 and 72 hpf. <t>cDNA</t> from total <t>RNA</t> was amplified using primers to ZF Brn-3a and Brn-3b and a variation in mRNA levels was corrected using ZF GAPDH. Values were expressed as fold induction relative to expression at 24 h (set at 1). ( b ) Representative western blot analysis showing single protein band for both Brn-3a and Brn-3b in extracts from adult ZF compared with adult mouse heart (M), used as a positive control. MW markers indicate the protein size and gamma ( γ ) tubulin was used to control for variation in total protein. ( c ) Representative images showing whole-mount immunostaining for (i) Brn-3a or (ii) Brn-3b (green; top panels) in ZF hearts at 72 hpf. Co-staining with tropomyosin (red; middle panels) indicate cardiomyocytes in the developing heart. Lower panel shows merge with bright field image. V, A, P (indicated by arrow). ( d ) Representative images of DAB-immunostained ZF embryos sections at 72 hpf. Protein localisation is seen as dark brown staining in ventricles (indicated by arrow), identified by TM, also shows Brn-3a and Brn-3b expression. × 40 magnification. A, atria; hpf, hours post fertilisation; MW, molecular weight; P, pericardial sac; TM, tropomyosin; V, ventricle; ZF, zebrafish heart
    Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 724 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcriptase/product/Thermo Fisher
    Average 91 stars, based on 724 article reviews
    Price from $9.99 to $1999.99
    reverse transcriptase - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    Image Search Results


    ( a ) Results of qRT-PCR showing (i) Brn-3a and (ii) Brn-3b mRNA levels in the developing zebrafish at 24, 48 and 72 hpf. cDNA from total RNA was amplified using primers to ZF Brn-3a and Brn-3b and a variation in mRNA levels was corrected using ZF GAPDH. Values were expressed as fold induction relative to expression at 24 h (set at 1). ( b ) Representative western blot analysis showing single protein band for both Brn-3a and Brn-3b in extracts from adult ZF compared with adult mouse heart (M), used as a positive control. MW markers indicate the protein size and gamma ( γ ) tubulin was used to control for variation in total protein. ( c ) Representative images showing whole-mount immunostaining for (i) Brn-3a or (ii) Brn-3b (green; top panels) in ZF hearts at 72 hpf. Co-staining with tropomyosin (red; middle panels) indicate cardiomyocytes in the developing heart. Lower panel shows merge with bright field image. V, A, P (indicated by arrow). ( d ) Representative images of DAB-immunostained ZF embryos sections at 72 hpf. Protein localisation is seen as dark brown staining in ventricles (indicated by arrow), identified by TM, also shows Brn-3a and Brn-3b expression. × 40 magnification. A, atria; hpf, hours post fertilisation; MW, molecular weight; P, pericardial sac; TM, tropomyosin; V, ventricle; ZF, zebrafish heart

    Journal: Cell Death & Disease

    Article Title: Essential but partially redundant roles for POU4F1/Brn-3a and POU4F2/Brn-3b transcription factors in the developing heart

    doi: 10.1038/cddis.2017.185

    Figure Lengend Snippet: ( a ) Results of qRT-PCR showing (i) Brn-3a and (ii) Brn-3b mRNA levels in the developing zebrafish at 24, 48 and 72 hpf. cDNA from total RNA was amplified using primers to ZF Brn-3a and Brn-3b and a variation in mRNA levels was corrected using ZF GAPDH. Values were expressed as fold induction relative to expression at 24 h (set at 1). ( b ) Representative western blot analysis showing single protein band for both Brn-3a and Brn-3b in extracts from adult ZF compared with adult mouse heart (M), used as a positive control. MW markers indicate the protein size and gamma ( γ ) tubulin was used to control for variation in total protein. ( c ) Representative images showing whole-mount immunostaining for (i) Brn-3a or (ii) Brn-3b (green; top panels) in ZF hearts at 72 hpf. Co-staining with tropomyosin (red; middle panels) indicate cardiomyocytes in the developing heart. Lower panel shows merge with bright field image. V, A, P (indicated by arrow). ( d ) Representative images of DAB-immunostained ZF embryos sections at 72 hpf. Protein localisation is seen as dark brown staining in ventricles (indicated by arrow), identified by TM, also shows Brn-3a and Brn-3b expression. × 40 magnification. A, atria; hpf, hours post fertilisation; MW, molecular weight; P, pericardial sac; TM, tropomyosin; V, ventricle; ZF, zebrafish heart

    Article Snippet: RNA was quantified (NanoDrop 1000 spectrophotometer, Thermo Fisher Scientific, Paisley, UK) and cDNA synthesis (20–50 μ l reaction) was carried out using RNA Superscript II Reverse Transcriptase (Invitrogen).

    Techniques: Quantitative RT-PCR, Amplification, Expressing, Western Blot, Positive Control, Immunostaining, Staining, Molecular Weight

    Full-length L1Rn elements are transcribed in RCL cells. ( A ) Structure of L1Rn. A functional full-length L1Rn element is characterized by two ORFs flanked by 5′ and 3′ UTRs. The bipartite 5′ UTR consists of a monomer, which can be tandemly repeated, and a non-repeated tether (t). The most 5′ monomer is only partially duplicated (black truncated box with arrowhead) in all genomic rat elements identified so far. Horizontal arrows indicate the binding sites of the oligonucleotides Pr-L1Rn1 and Pr-L1Rn2 used to amplify cDNAs generated from full-length transcripts. The binding site of the 500-bp digoxigenin-labeled probe used to detect L1-specific transcripts is localized at the 5′ end of ORF2 (black bar). Open bars represent ORF1- and ORF2-encoded polypeptides against which monoclonal antibodies were raised. The polypeptides are covering amino acid positions 144–402 and 292–480 of ORF1p and ORF2p, respectively (accession no. DQ100480). LPR, length polymorphism region; GrPPT, G-rich polypurine tract; A n , A-rich 3′ tract; EN, endonuclease; RT, reverse transcriptase; C, cysteine-rich motif. ( B ) L1Rn transcriptional products in RCL cells. PolyA + RNA was isolated from RCL cells that had reached the maximum population density of ∼10 6 cells/ml (lane 2) and from cells that were UV-irradiated (lane 1). Two microgram of each RNA were separated by agarose gel electrophoresis and subjected to northern blot analysis using a 500-bp probe ( Figure 1 A). (C) Schematic structures of 10 L1Rn cDNAs synthesized from poly(A) + RNA from UV-irradiated RCL cells. cDNAs are flanked by primer sequences Pr-L1Rn1 and Pr-L1Rn2. Names of the resulting cDNAs are listed on the left, while accession numbers are specified on the right. Termination codons within ORF2 sequences are indicated by vertical arrows. Two deletions in the ORF2-coding region of cDNA L1-17 covering 103 and 220 nts, respectively, are indicated by interrupted bars.

    Journal: Nucleic Acids Research

    Article Title: Functional endogenous LINE-1 retrotransposons are expressed and mobilized in rat chloroleukemia cells

    doi: 10.1093/nar/gkm1045

    Figure Lengend Snippet: Full-length L1Rn elements are transcribed in RCL cells. ( A ) Structure of L1Rn. A functional full-length L1Rn element is characterized by two ORFs flanked by 5′ and 3′ UTRs. The bipartite 5′ UTR consists of a monomer, which can be tandemly repeated, and a non-repeated tether (t). The most 5′ monomer is only partially duplicated (black truncated box with arrowhead) in all genomic rat elements identified so far. Horizontal arrows indicate the binding sites of the oligonucleotides Pr-L1Rn1 and Pr-L1Rn2 used to amplify cDNAs generated from full-length transcripts. The binding site of the 500-bp digoxigenin-labeled probe used to detect L1-specific transcripts is localized at the 5′ end of ORF2 (black bar). Open bars represent ORF1- and ORF2-encoded polypeptides against which monoclonal antibodies were raised. The polypeptides are covering amino acid positions 144–402 and 292–480 of ORF1p and ORF2p, respectively (accession no. DQ100480). LPR, length polymorphism region; GrPPT, G-rich polypurine tract; A n , A-rich 3′ tract; EN, endonuclease; RT, reverse transcriptase; C, cysteine-rich motif. ( B ) L1Rn transcriptional products in RCL cells. PolyA + RNA was isolated from RCL cells that had reached the maximum population density of ∼10 6 cells/ml (lane 2) and from cells that were UV-irradiated (lane 1). Two microgram of each RNA were separated by agarose gel electrophoresis and subjected to northern blot analysis using a 500-bp probe ( Figure 1 A). (C) Schematic structures of 10 L1Rn cDNAs synthesized from poly(A) + RNA from UV-irradiated RCL cells. cDNAs are flanked by primer sequences Pr-L1Rn1 and Pr-L1Rn2. Names of the resulting cDNAs are listed on the left, while accession numbers are specified on the right. Termination codons within ORF2 sequences are indicated by vertical arrows. Two deletions in the ORF2-coding region of cDNA L1-17 covering 103 and 220 nts, respectively, are indicated by interrupted bars.

    Article Snippet: Cloning of transcribed L1Rn sequences from RCL cells Total and poly(A)+ RNA were prepared from control and irradiated RCL cells with the RNeasy and Oligotex mRNA kits (Qiagen), respectively. cDNA was synthesized from poly(A)+ RNA employing SuperScript™ II reverse transcriptase (Invitrogen) and oligo(dT) primer.

    Techniques: Functional Assay, Binding Assay, Generated, Labeling, Isolation, Irradiation, Agarose Gel Electrophoresis, Northern Blot, Synthesized