superscript ii reverse transcriptase Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher rna superscript ii reverse transcriptase
    ( a ) Results of qRT-PCR showing (i) Brn-3a and (ii) Brn-3b mRNA levels in the developing zebrafish at 24, 48 and 72 hpf. <t>cDNA</t> from total <t>RNA</t> was amplified using primers to ZF Brn-3a and Brn-3b and a variation in mRNA levels was corrected using ZF GAPDH. Values were expressed as fold induction relative to expression at 24 h (set at 1). ( b ) Representative western blot analysis showing single protein band for both Brn-3a and Brn-3b in extracts from adult ZF compared with adult mouse heart (M), used as a positive control. MW markers indicate the protein size and gamma ( γ ) tubulin was used to control for variation in total protein. ( c ) Representative images showing whole-mount immunostaining for (i) Brn-3a or (ii) Brn-3b (green; top panels) in ZF hearts at 72 hpf. Co-staining with tropomyosin (red; middle panels) indicate cardiomyocytes in the developing heart. Lower panel shows merge with bright field image. V, A, P (indicated by arrow). ( d ) Representative images of DAB-immunostained ZF embryos sections at 72 hpf. Protein localisation is seen as dark brown staining in ventricles (indicated by arrow), identified by TM, also shows Brn-3a and Brn-3b expression. × 40 magnification. A, atria; hpf, hours post fertilisation; MW, molecular weight; P, pericardial sac; TM, tropomyosin; V, ventricle; ZF, zebrafish heart
    Rna Superscript Ii Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5778 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna superscript ii reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 5778 article reviews
    Price from $9.99 to $1999.99
    rna superscript ii reverse transcriptase - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher reverse transcriptase
    ( a ) Results of qRT-PCR showing (i) Brn-3a and (ii) Brn-3b mRNA levels in the developing zebrafish at 24, 48 and 72 hpf. <t>cDNA</t> from total <t>RNA</t> was amplified using primers to ZF Brn-3a and Brn-3b and a variation in mRNA levels was corrected using ZF GAPDH. Values were expressed as fold induction relative to expression at 24 h (set at 1). ( b ) Representative western blot analysis showing single protein band for both Brn-3a and Brn-3b in extracts from adult ZF compared with adult mouse heart (M), used as a positive control. MW markers indicate the protein size and gamma ( γ ) tubulin was used to control for variation in total protein. ( c ) Representative images showing whole-mount immunostaining for (i) Brn-3a or (ii) Brn-3b (green; top panels) in ZF hearts at 72 hpf. Co-staining with tropomyosin (red; middle panels) indicate cardiomyocytes in the developing heart. Lower panel shows merge with bright field image. V, A, P (indicated by arrow). ( d ) Representative images of DAB-immunostained ZF embryos sections at 72 hpf. Protein localisation is seen as dark brown staining in ventricles (indicated by arrow), identified by TM, also shows Brn-3a and Brn-3b expression. × 40 magnification. A, atria; hpf, hours post fertilisation; MW, molecular weight; P, pericardial sac; TM, tropomyosin; V, ventricle; ZF, zebrafish heart
    Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 416 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 416 article reviews
    Price from $9.99 to $1999.99
    reverse transcriptase - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher superscript ii reverse transcriptase kit
    ( a ) Results of qRT-PCR showing (i) Brn-3a and (ii) Brn-3b mRNA levels in the developing zebrafish at 24, 48 and 72 hpf. <t>cDNA</t> from total <t>RNA</t> was amplified using primers to ZF Brn-3a and Brn-3b and a variation in mRNA levels was corrected using ZF GAPDH. Values were expressed as fold induction relative to expression at 24 h (set at 1). ( b ) Representative western blot analysis showing single protein band for both Brn-3a and Brn-3b in extracts from adult ZF compared with adult mouse heart (M), used as a positive control. MW markers indicate the protein size and gamma ( γ ) tubulin was used to control for variation in total protein. ( c ) Representative images showing whole-mount immunostaining for (i) Brn-3a or (ii) Brn-3b (green; top panels) in ZF hearts at 72 hpf. Co-staining with tropomyosin (red; middle panels) indicate cardiomyocytes in the developing heart. Lower panel shows merge with bright field image. V, A, P (indicated by arrow). ( d ) Representative images of DAB-immunostained ZF embryos sections at 72 hpf. Protein localisation is seen as dark brown staining in ventricles (indicated by arrow), identified by TM, also shows Brn-3a and Brn-3b expression. × 40 magnification. A, atria; hpf, hours post fertilisation; MW, molecular weight; P, pericardial sac; TM, tropomyosin; V, ventricle; ZF, zebrafish heart
    Superscript Ii Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4958 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript ii reverse transcriptase kit/product/Thermo Fisher
    Average 99 stars, based on 4958 article reviews
    Price from $9.99 to $1999.99
    superscript ii reverse transcriptase kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    ( a ) Results of qRT-PCR showing (i) Brn-3a and (ii) Brn-3b mRNA levels in the developing zebrafish at 24, 48 and 72 hpf. cDNA from total RNA was amplified using primers to ZF Brn-3a and Brn-3b and a variation in mRNA levels was corrected using ZF GAPDH. Values were expressed as fold induction relative to expression at 24 h (set at 1). ( b ) Representative western blot analysis showing single protein band for both Brn-3a and Brn-3b in extracts from adult ZF compared with adult mouse heart (M), used as a positive control. MW markers indicate the protein size and gamma ( γ ) tubulin was used to control for variation in total protein. ( c ) Representative images showing whole-mount immunostaining for (i) Brn-3a or (ii) Brn-3b (green; top panels) in ZF hearts at 72 hpf. Co-staining with tropomyosin (red; middle panels) indicate cardiomyocytes in the developing heart. Lower panel shows merge with bright field image. V, A, P (indicated by arrow). ( d ) Representative images of DAB-immunostained ZF embryos sections at 72 hpf. Protein localisation is seen as dark brown staining in ventricles (indicated by arrow), identified by TM, also shows Brn-3a and Brn-3b expression. × 40 magnification. A, atria; hpf, hours post fertilisation; MW, molecular weight; P, pericardial sac; TM, tropomyosin; V, ventricle; ZF, zebrafish heart

    Journal: Cell Death & Disease

    Article Title: Essential but partially redundant roles for POU4F1/Brn-3a and POU4F2/Brn-3b transcription factors in the developing heart

    doi: 10.1038/cddis.2017.185

    Figure Lengend Snippet: ( a ) Results of qRT-PCR showing (i) Brn-3a and (ii) Brn-3b mRNA levels in the developing zebrafish at 24, 48 and 72 hpf. cDNA from total RNA was amplified using primers to ZF Brn-3a and Brn-3b and a variation in mRNA levels was corrected using ZF GAPDH. Values were expressed as fold induction relative to expression at 24 h (set at 1). ( b ) Representative western blot analysis showing single protein band for both Brn-3a and Brn-3b in extracts from adult ZF compared with adult mouse heart (M), used as a positive control. MW markers indicate the protein size and gamma ( γ ) tubulin was used to control for variation in total protein. ( c ) Representative images showing whole-mount immunostaining for (i) Brn-3a or (ii) Brn-3b (green; top panels) in ZF hearts at 72 hpf. Co-staining with tropomyosin (red; middle panels) indicate cardiomyocytes in the developing heart. Lower panel shows merge with bright field image. V, A, P (indicated by arrow). ( d ) Representative images of DAB-immunostained ZF embryos sections at 72 hpf. Protein localisation is seen as dark brown staining in ventricles (indicated by arrow), identified by TM, also shows Brn-3a and Brn-3b expression. × 40 magnification. A, atria; hpf, hours post fertilisation; MW, molecular weight; P, pericardial sac; TM, tropomyosin; V, ventricle; ZF, zebrafish heart

    Article Snippet: RNA was quantified (NanoDrop 1000 spectrophotometer, Thermo Fisher Scientific, Paisley, UK) and cDNA synthesis (20–50 μ l reaction) was carried out using RNA Superscript II Reverse Transcriptase (Invitrogen).

    Techniques: Quantitative RT-PCR, Amplification, Expressing, Western Blot, Positive Control, Immunostaining, Staining, Molecular Weight

    Full-length L1Rn elements are transcribed in RCL cells. ( A ) Structure of L1Rn. A functional full-length L1Rn element is characterized by two ORFs flanked by 5′ and 3′ UTRs. The bipartite 5′ UTR consists of a monomer, which can be tandemly repeated, and a non-repeated tether (t). The most 5′ monomer is only partially duplicated (black truncated box with arrowhead) in all genomic rat elements identified so far. Horizontal arrows indicate the binding sites of the oligonucleotides Pr-L1Rn1 and Pr-L1Rn2 used to amplify cDNAs generated from full-length transcripts. The binding site of the 500-bp digoxigenin-labeled probe used to detect L1-specific transcripts is localized at the 5′ end of ORF2 (black bar). Open bars represent ORF1- and ORF2-encoded polypeptides against which monoclonal antibodies were raised. The polypeptides are covering amino acid positions 144–402 and 292–480 of ORF1p and ORF2p, respectively (accession no. DQ100480). LPR, length polymorphism region; GrPPT, G-rich polypurine tract; A n , A-rich 3′ tract; EN, endonuclease; RT, reverse transcriptase; C, cysteine-rich motif. ( B ) L1Rn transcriptional products in RCL cells. PolyA + RNA was isolated from RCL cells that had reached the maximum population density of ∼10 6 cells/ml (lane 2) and from cells that were UV-irradiated (lane 1). Two microgram of each RNA were separated by agarose gel electrophoresis and subjected to northern blot analysis using a 500-bp probe ( Figure 1 A). (C) Schematic structures of 10 L1Rn cDNAs synthesized from poly(A) + RNA from UV-irradiated RCL cells. cDNAs are flanked by primer sequences Pr-L1Rn1 and Pr-L1Rn2. Names of the resulting cDNAs are listed on the left, while accession numbers are specified on the right. Termination codons within ORF2 sequences are indicated by vertical arrows. Two deletions in the ORF2-coding region of cDNA L1-17 covering 103 and 220 nts, respectively, are indicated by interrupted bars.

    Journal: Nucleic Acids Research

    Article Title: Functional endogenous LINE-1 retrotransposons are expressed and mobilized in rat chloroleukemia cells

    doi: 10.1093/nar/gkm1045

    Figure Lengend Snippet: Full-length L1Rn elements are transcribed in RCL cells. ( A ) Structure of L1Rn. A functional full-length L1Rn element is characterized by two ORFs flanked by 5′ and 3′ UTRs. The bipartite 5′ UTR consists of a monomer, which can be tandemly repeated, and a non-repeated tether (t). The most 5′ monomer is only partially duplicated (black truncated box with arrowhead) in all genomic rat elements identified so far. Horizontal arrows indicate the binding sites of the oligonucleotides Pr-L1Rn1 and Pr-L1Rn2 used to amplify cDNAs generated from full-length transcripts. The binding site of the 500-bp digoxigenin-labeled probe used to detect L1-specific transcripts is localized at the 5′ end of ORF2 (black bar). Open bars represent ORF1- and ORF2-encoded polypeptides against which monoclonal antibodies were raised. The polypeptides are covering amino acid positions 144–402 and 292–480 of ORF1p and ORF2p, respectively (accession no. DQ100480). LPR, length polymorphism region; GrPPT, G-rich polypurine tract; A n , A-rich 3′ tract; EN, endonuclease; RT, reverse transcriptase; C, cysteine-rich motif. ( B ) L1Rn transcriptional products in RCL cells. PolyA + RNA was isolated from RCL cells that had reached the maximum population density of ∼10 6 cells/ml (lane 2) and from cells that were UV-irradiated (lane 1). Two microgram of each RNA were separated by agarose gel electrophoresis and subjected to northern blot analysis using a 500-bp probe ( Figure 1 A). (C) Schematic structures of 10 L1Rn cDNAs synthesized from poly(A) + RNA from UV-irradiated RCL cells. cDNAs are flanked by primer sequences Pr-L1Rn1 and Pr-L1Rn2. Names of the resulting cDNAs are listed on the left, while accession numbers are specified on the right. Termination codons within ORF2 sequences are indicated by vertical arrows. Two deletions in the ORF2-coding region of cDNA L1-17 covering 103 and 220 nts, respectively, are indicated by interrupted bars.

    Article Snippet: Cloning of transcribed L1Rn sequences from RCL cells Total and poly(A)+ RNA were prepared from control and irradiated RCL cells with the RNeasy and Oligotex mRNA kits (Qiagen), respectively. cDNA was synthesized from poly(A)+ RNA employing SuperScript™ II reverse transcriptase (Invitrogen) and oligo(dT) primer.

    Techniques: Functional Assay, Binding Assay, Generated, Labeling, Isolation, Irradiation, Agarose Gel Electrophoresis, Northern Blot, Synthesized

    Analysis of tumor samples for expression of antigens from stable cell lines confirmed expression in the tumors. (a) Protein was extracted from primary tumor tissues, and the concentration was calculated. Tissues were homogenized by using a tissue tearer prior to processing for protein extraction. Portions (100 μg) of lysate were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto a nitrocellulose membrane. Equal loading of samples was confirmed with Ponceau-S staining of the membrane in all cases. The EBNA1 and Myc-tagged Nm23-H1 or EBNA3C were analyzed as reported with the use of anti-EBV human serum and anti-Myc antibody, respectively. (b) Total RNA was isolated from tissues by using TRIZOL reagent. Tissues were homogenized by using a tissue tearer prior to processing for RNA isolation. RT was carried performed with SuperScript II RNase H reverse transcriptase. followed by PCR with specific primers to detect the desired transcript. The Nm23-H1-Myc transcript was amplified by using the forward primer 5′-GATTACACGAGCTGTGCTCA-3′ and the reverse primer 5′-TTCGCTAGCCAAGTCTTCTT-3′ designed to amplify the junction sequence between Nm23-H1 and Myc tag. The EBNA3C-Myc transcript was amplified by using the forward primer 5′-CGGGATCCGGAAGGAACCATGGCCA-3′ and the reverse primer 5′-GAATTCTCCTGTCATTTCATAGATCCA-3′. The EBNA1 transcript was amplified by using the forward primer 5′-CGGGATCCGGAAGGAACCATGGCCA-3′ and the reverse primer 5′-GAATTCTCCTGTCATTTCATAGATCCA-3′. Amplification products were resolved in 1.5% agarose gels.

    Journal: Journal of Virology

    Article Title: Epstein-Barr Virus Latent Nuclear Antigens Can Induce Metastasis in a Nude Mouse Model ▿

    doi: 10.1128/JVI.00886-07

    Figure Lengend Snippet: Analysis of tumor samples for expression of antigens from stable cell lines confirmed expression in the tumors. (a) Protein was extracted from primary tumor tissues, and the concentration was calculated. Tissues were homogenized by using a tissue tearer prior to processing for protein extraction. Portions (100 μg) of lysate were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto a nitrocellulose membrane. Equal loading of samples was confirmed with Ponceau-S staining of the membrane in all cases. The EBNA1 and Myc-tagged Nm23-H1 or EBNA3C were analyzed as reported with the use of anti-EBV human serum and anti-Myc antibody, respectively. (b) Total RNA was isolated from tissues by using TRIZOL reagent. Tissues were homogenized by using a tissue tearer prior to processing for RNA isolation. RT was carried performed with SuperScript II RNase H reverse transcriptase. followed by PCR with specific primers to detect the desired transcript. The Nm23-H1-Myc transcript was amplified by using the forward primer 5′-GATTACACGAGCTGTGCTCA-3′ and the reverse primer 5′-TTCGCTAGCCAAGTCTTCTT-3′ designed to amplify the junction sequence between Nm23-H1 and Myc tag. The EBNA3C-Myc transcript was amplified by using the forward primer 5′-CGGGATCCGGAAGGAACCATGGCCA-3′ and the reverse primer 5′-GAATTCTCCTGTCATTTCATAGATCCA-3′. The EBNA1 transcript was amplified by using the forward primer 5′-CGGGATCCGGAAGGAACCATGGCCA-3′ and the reverse primer 5′-GAATTCTCCTGTCATTTCATAGATCCA-3′. Amplification products were resolved in 1.5% agarose gels.

    Article Snippet: RT was carried out with SuperScript II RNase H-reverse transcriptase (Life Technologies, Gaithersburg, MD).

    Techniques: Expressing, Stable Transfection, Concentration Assay, Protein Extraction, Polyacrylamide Gel Electrophoresis, Staining, Isolation, Polymerase Chain Reaction, Amplification, Sequencing