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  • 99
    Thermo Fisher invitrogen superscript ii
    Tissue distribution of mouse CARK gene. Total RNAs were extracted from different mouse tissues using Trizol reagent. Reverse transcribed <t>cDNA</t> including liver, spleen, lung, brain, kidney, skeletal muscle, testis, heart (C57BL/6J), heart (Balb/C) and heart (KM; lanes 1–10) were amplified to give rise to the 261-bp-specific fragment of CARK . The 261 bp band was only detected in mouse heart from three different strains (C57BL/6J, Balb/C and KM). To control for the amount of cDNA in each reaction, a parallel <t>PCR</t> reaction using the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was carried out. RT-PCR products were analyzed on 2% agarose gel.
    Invitrogen Superscript Ii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1234 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher superscript ii
    Tissue distribution of mouse CARK gene. Total RNAs were extracted from different mouse tissues using Trizol reagent. Reverse transcribed <t>cDNA</t> including liver, spleen, lung, brain, kidney, skeletal muscle, testis, heart (C57BL/6J), heart (Balb/C) and heart (KM; lanes 1–10) were amplified to give rise to the 261-bp-specific fragment of CARK . The 261 bp band was only detected in mouse heart from three different strains (C57BL/6J, Balb/C and KM). To control for the amount of cDNA in each reaction, a parallel <t>PCR</t> reaction using the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was carried out. RT-PCR products were analyzed on 2% agarose gel.
    Superscript Ii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 37020 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher superscript ii rnaseh
    Tissue distribution of mouse CARK gene. Total RNAs were extracted from different mouse tissues using Trizol reagent. Reverse transcribed <t>cDNA</t> including liver, spleen, lung, brain, kidney, skeletal muscle, testis, heart (C57BL/6J), heart (Balb/C) and heart (KM; lanes 1–10) were amplified to give rise to the 261-bp-specific fragment of CARK . The 261 bp band was only detected in mouse heart from three different strains (C57BL/6J, Balb/C and KM). To control for the amount of cDNA in each reaction, a parallel <t>PCR</t> reaction using the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was carried out. RT-PCR products were analyzed on 2% agarose gel.
    Superscript Ii Rnaseh, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher superscript ii kit
    Tissue distribution of mouse CARK gene. Total RNAs were extracted from different mouse tissues using Trizol reagent. Reverse transcribed <t>cDNA</t> including liver, spleen, lung, brain, kidney, skeletal muscle, testis, heart (C57BL/6J), heart (Balb/C) and heart (KM; lanes 1–10) were amplified to give rise to the 261-bp-specific fragment of CARK . The 261 bp band was only detected in mouse heart from three different strains (C57BL/6J, Balb/C and KM). To control for the amount of cDNA in each reaction, a parallel <t>PCR</t> reaction using the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was carried out. RT-PCR products were analyzed on 2% agarose gel.
    Superscript Ii Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4220 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Thermo Fisher superscript ii rna polymerase
    Runx2 deficient mice have higher levels of BMP-3B. (a) Total <t>RNA</t> from mesenchymal cells of calvarial tissue from wild type and Runx2 -/- (17.5 dpc) was hybridized with osteogenesis-related <t>cDNA</t> array. Signal of BMP-3B on blots is indicated by an arrow. Control genes (GAPDH, cyclophilin A) are shown in lower panel. ( b ) BMP-3B mRNA levels (normalized to GAPDH) in calvarial mesenchymal cells from wild type (WT) and Runx2 -/- animals as detected by qRT-PCR analysis. ( c ) Primary calvarial cells from Runx2 deficient animals were transduced with empty vector (EV) or Runx2 expressing adenovirus with increasing multiplicity of infection (MOI 10 and 20) for 48 hr. Total RNA isolated from the infected cells was utilized to examine BMP-3B mRNA as detected by qRT-PCR analysis. ( d ) Runx2 mRNA expression levels were examined by qRT-PCR and the gene expression levels were normalized to 28S internal control. The gene expression levels were calibrated to IMR-90 cells. ( e ) BMP-3B mRNA expression levels were examined by qRT-PCR and the gene expression levels were normalized to 28S internal control. The BMP-3B gene expression levels were calibrated to Runx2 levels in IMR-90 cells. ( f ) Runx2 protein levels were examined in nuclear extracts of normal lung fibroblast cells (IMR-90 and WI-38) and lung cancer cell lines (H720 and H1299) by immunoblotting with Runx2 monoclonal antibody. Runx2 expression levels were normalized to internal control LaminA/C protein. ( g ) Runx2 intracellular localization was determined by immunofluorescence of endogenous Runx2 protein in WI-38 and H1299 cells. The punctate (Alexa 488, green) signal shows Runx2 staining while nuclei are revealed by 4, 6-diamidino-2-phenylindole (DAPI, blue) staining.
    Superscript Ii Rna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher superscript ii rt system
    Runx2 deficient mice have higher levels of BMP-3B. (a) Total <t>RNA</t> from mesenchymal cells of calvarial tissue from wild type and Runx2 -/- (17.5 dpc) was hybridized with osteogenesis-related <t>cDNA</t> array. Signal of BMP-3B on blots is indicated by an arrow. Control genes (GAPDH, cyclophilin A) are shown in lower panel. ( b ) BMP-3B mRNA levels (normalized to GAPDH) in calvarial mesenchymal cells from wild type (WT) and Runx2 -/- animals as detected by qRT-PCR analysis. ( c ) Primary calvarial cells from Runx2 deficient animals were transduced with empty vector (EV) or Runx2 expressing adenovirus with increasing multiplicity of infection (MOI 10 and 20) for 48 hr. Total RNA isolated from the infected cells was utilized to examine BMP-3B mRNA as detected by qRT-PCR analysis. ( d ) Runx2 mRNA expression levels were examined by qRT-PCR and the gene expression levels were normalized to 28S internal control. The gene expression levels were calibrated to IMR-90 cells. ( e ) BMP-3B mRNA expression levels were examined by qRT-PCR and the gene expression levels were normalized to 28S internal control. The BMP-3B gene expression levels were calibrated to Runx2 levels in IMR-90 cells. ( f ) Runx2 protein levels were examined in nuclear extracts of normal lung fibroblast cells (IMR-90 and WI-38) and lung cancer cell lines (H720 and H1299) by immunoblotting with Runx2 monoclonal antibody. Runx2 expression levels were normalized to internal control LaminA/C protein. ( g ) Runx2 intracellular localization was determined by immunofluorescence of endogenous Runx2 protein in WI-38 and H1299 cells. The punctate (Alexa 488, green) signal shows Runx2 staining while nuclei are revealed by 4, 6-diamidino-2-phenylindole (DAPI, blue) staining.
    Superscript Ii Rt System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Promega superscript ii
    Runx2 deficient mice have higher levels of BMP-3B. (a) Total <t>RNA</t> from mesenchymal cells of calvarial tissue from wild type and Runx2 -/- (17.5 dpc) was hybridized with osteogenesis-related <t>cDNA</t> array. Signal of BMP-3B on blots is indicated by an arrow. Control genes (GAPDH, cyclophilin A) are shown in lower panel. ( b ) BMP-3B mRNA levels (normalized to GAPDH) in calvarial mesenchymal cells from wild type (WT) and Runx2 -/- animals as detected by qRT-PCR analysis. ( c ) Primary calvarial cells from Runx2 deficient animals were transduced with empty vector (EV) or Runx2 expressing adenovirus with increasing multiplicity of infection (MOI 10 and 20) for 48 hr. Total RNA isolated from the infected cells was utilized to examine BMP-3B mRNA as detected by qRT-PCR analysis. ( d ) Runx2 mRNA expression levels were examined by qRT-PCR and the gene expression levels were normalized to 28S internal control. The gene expression levels were calibrated to IMR-90 cells. ( e ) BMP-3B mRNA expression levels were examined by qRT-PCR and the gene expression levels were normalized to 28S internal control. The BMP-3B gene expression levels were calibrated to Runx2 levels in IMR-90 cells. ( f ) Runx2 protein levels were examined in nuclear extracts of normal lung fibroblast cells (IMR-90 and WI-38) and lung cancer cell lines (H720 and H1299) by immunoblotting with Runx2 monoclonal antibody. Runx2 expression levels were normalized to internal control LaminA/C protein. ( g ) Runx2 intracellular localization was determined by immunofluorescence of endogenous Runx2 protein in WI-38 and H1299 cells. The punctate (Alexa 488, green) signal shows Runx2 staining while nuclei are revealed by 4, 6-diamidino-2-phenylindole (DAPI, blue) staining.
    Superscript Ii, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 162 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Roche superscript ii
    Runx2 deficient mice have higher levels of BMP-3B. (a) Total <t>RNA</t> from mesenchymal cells of calvarial tissue from wild type and Runx2 -/- (17.5 dpc) was hybridized with osteogenesis-related <t>cDNA</t> array. Signal of BMP-3B on blots is indicated by an arrow. Control genes (GAPDH, cyclophilin A) are shown in lower panel. ( b ) BMP-3B mRNA levels (normalized to GAPDH) in calvarial mesenchymal cells from wild type (WT) and Runx2 -/- animals as detected by qRT-PCR analysis. ( c ) Primary calvarial cells from Runx2 deficient animals were transduced with empty vector (EV) or Runx2 expressing adenovirus with increasing multiplicity of infection (MOI 10 and 20) for 48 hr. Total RNA isolated from the infected cells was utilized to examine BMP-3B mRNA as detected by qRT-PCR analysis. ( d ) Runx2 mRNA expression levels were examined by qRT-PCR and the gene expression levels were normalized to 28S internal control. The gene expression levels were calibrated to IMR-90 cells. ( e ) BMP-3B mRNA expression levels were examined by qRT-PCR and the gene expression levels were normalized to 28S internal control. The BMP-3B gene expression levels were calibrated to Runx2 levels in IMR-90 cells. ( f ) Runx2 protein levels were examined in nuclear extracts of normal lung fibroblast cells (IMR-90 and WI-38) and lung cancer cell lines (H720 and H1299) by immunoblotting with Runx2 monoclonal antibody. Runx2 expression levels were normalized to internal control LaminA/C protein. ( g ) Runx2 intracellular localization was determined by immunofluorescence of endogenous Runx2 protein in WI-38 and H1299 cells. The punctate (Alexa 488, green) signal shows Runx2 staining while nuclei are revealed by 4, 6-diamidino-2-phenylindole (DAPI, blue) staining.
    Superscript Ii, supplied by Roche, used in various techniques. Bioz Stars score: 96/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Stratagene superscript ii
    Runx2 deficient mice have higher levels of BMP-3B. (a) Total <t>RNA</t> from mesenchymal cells of calvarial tissue from wild type and Runx2 -/- (17.5 dpc) was hybridized with osteogenesis-related <t>cDNA</t> array. Signal of BMP-3B on blots is indicated by an arrow. Control genes (GAPDH, cyclophilin A) are shown in lower panel. ( b ) BMP-3B mRNA levels (normalized to GAPDH) in calvarial mesenchymal cells from wild type (WT) and Runx2 -/- animals as detected by qRT-PCR analysis. ( c ) Primary calvarial cells from Runx2 deficient animals were transduced with empty vector (EV) or Runx2 expressing adenovirus with increasing multiplicity of infection (MOI 10 and 20) for 48 hr. Total RNA isolated from the infected cells was utilized to examine BMP-3B mRNA as detected by qRT-PCR analysis. ( d ) Runx2 mRNA expression levels were examined by qRT-PCR and the gene expression levels were normalized to 28S internal control. The gene expression levels were calibrated to IMR-90 cells. ( e ) BMP-3B mRNA expression levels were examined by qRT-PCR and the gene expression levels were normalized to 28S internal control. The BMP-3B gene expression levels were calibrated to Runx2 levels in IMR-90 cells. ( f ) Runx2 protein levels were examined in nuclear extracts of normal lung fibroblast cells (IMR-90 and WI-38) and lung cancer cell lines (H720 and H1299) by immunoblotting with Runx2 monoclonal antibody. Runx2 expression levels were normalized to internal control LaminA/C protein. ( g ) Runx2 intracellular localization was determined by immunofluorescence of endogenous Runx2 protein in WI-38 and H1299 cells. The punctate (Alexa 488, green) signal shows Runx2 staining while nuclei are revealed by 4, 6-diamidino-2-phenylindole (DAPI, blue) staining.
    Superscript Ii, supplied by Stratagene, used in various techniques. Bioz Stars score: 84/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    TaKaRa superscript ii
    Runx2 deficient mice have higher levels of BMP-3B. (a) Total <t>RNA</t> from mesenchymal cells of calvarial tissue from wild type and Runx2 -/- (17.5 dpc) was hybridized with osteogenesis-related <t>cDNA</t> array. Signal of BMP-3B on blots is indicated by an arrow. Control genes (GAPDH, cyclophilin A) are shown in lower panel. ( b ) BMP-3B mRNA levels (normalized to GAPDH) in calvarial mesenchymal cells from wild type (WT) and Runx2 -/- animals as detected by qRT-PCR analysis. ( c ) Primary calvarial cells from Runx2 deficient animals were transduced with empty vector (EV) or Runx2 expressing adenovirus with increasing multiplicity of infection (MOI 10 and 20) for 48 hr. Total RNA isolated from the infected cells was utilized to examine BMP-3B mRNA as detected by qRT-PCR analysis. ( d ) Runx2 mRNA expression levels were examined by qRT-PCR and the gene expression levels were normalized to 28S internal control. The gene expression levels were calibrated to IMR-90 cells. ( e ) BMP-3B mRNA expression levels were examined by qRT-PCR and the gene expression levels were normalized to 28S internal control. The BMP-3B gene expression levels were calibrated to Runx2 levels in IMR-90 cells. ( f ) Runx2 protein levels were examined in nuclear extracts of normal lung fibroblast cells (IMR-90 and WI-38) and lung cancer cell lines (H720 and H1299) by immunoblotting with Runx2 monoclonal antibody. Runx2 expression levels were normalized to internal control LaminA/C protein. ( g ) Runx2 intracellular localization was determined by immunofluorescence of endogenous Runx2 protein in WI-38 and H1299 cells. The punctate (Alexa 488, green) signal shows Runx2 staining while nuclei are revealed by 4, 6-diamidino-2-phenylindole (DAPI, blue) staining.
    Superscript Ii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Vazyme Biotech Co superscript ii
    Runx2 deficient mice have higher levels of BMP-3B. (a) Total <t>RNA</t> from mesenchymal cells of calvarial tissue from wild type and Runx2 -/- (17.5 dpc) was hybridized with osteogenesis-related <t>cDNA</t> array. Signal of BMP-3B on blots is indicated by an arrow. Control genes (GAPDH, cyclophilin A) are shown in lower panel. ( b ) BMP-3B mRNA levels (normalized to GAPDH) in calvarial mesenchymal cells from wild type (WT) and Runx2 -/- animals as detected by qRT-PCR analysis. ( c ) Primary calvarial cells from Runx2 deficient animals were transduced with empty vector (EV) or Runx2 expressing adenovirus with increasing multiplicity of infection (MOI 10 and 20) for 48 hr. Total RNA isolated from the infected cells was utilized to examine BMP-3B mRNA as detected by qRT-PCR analysis. ( d ) Runx2 mRNA expression levels were examined by qRT-PCR and the gene expression levels were normalized to 28S internal control. The gene expression levels were calibrated to IMR-90 cells. ( e ) BMP-3B mRNA expression levels were examined by qRT-PCR and the gene expression levels were normalized to 28S internal control. The BMP-3B gene expression levels were calibrated to Runx2 levels in IMR-90 cells. ( f ) Runx2 protein levels were examined in nuclear extracts of normal lung fibroblast cells (IMR-90 and WI-38) and lung cancer cell lines (H720 and H1299) by immunoblotting with Runx2 monoclonal antibody. Runx2 expression levels were normalized to internal control LaminA/C protein. ( g ) Runx2 intracellular localization was determined by immunofluorescence of endogenous Runx2 protein in WI-38 and H1299 cells. The punctate (Alexa 488, green) signal shows Runx2 staining while nuclei are revealed by 4, 6-diamidino-2-phenylindole (DAPI, blue) staining.
    Superscript Ii, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 95/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher superscript ii rt protocol
    Runx2 deficient mice have higher levels of BMP-3B. (a) Total <t>RNA</t> from mesenchymal cells of calvarial tissue from wild type and Runx2 -/- (17.5 dpc) was hybridized with osteogenesis-related <t>cDNA</t> array. Signal of BMP-3B on blots is indicated by an arrow. Control genes (GAPDH, cyclophilin A) are shown in lower panel. ( b ) BMP-3B mRNA levels (normalized to GAPDH) in calvarial mesenchymal cells from wild type (WT) and Runx2 -/- animals as detected by qRT-PCR analysis. ( c ) Primary calvarial cells from Runx2 deficient animals were transduced with empty vector (EV) or Runx2 expressing adenovirus with increasing multiplicity of infection (MOI 10 and 20) for 48 hr. Total RNA isolated from the infected cells was utilized to examine BMP-3B mRNA as detected by qRT-PCR analysis. ( d ) Runx2 mRNA expression levels were examined by qRT-PCR and the gene expression levels were normalized to 28S internal control. The gene expression levels were calibrated to IMR-90 cells. ( e ) BMP-3B mRNA expression levels were examined by qRT-PCR and the gene expression levels were normalized to 28S internal control. The BMP-3B gene expression levels were calibrated to Runx2 levels in IMR-90 cells. ( f ) Runx2 protein levels were examined in nuclear extracts of normal lung fibroblast cells (IMR-90 and WI-38) and lung cancer cell lines (H720 and H1299) by immunoblotting with Runx2 monoclonal antibody. Runx2 expression levels were normalized to internal control LaminA/C protein. ( g ) Runx2 intracellular localization was determined by immunofluorescence of endogenous Runx2 protein in WI-38 and H1299 cells. The punctate (Alexa 488, green) signal shows Runx2 staining while nuclei are revealed by 4, 6-diamidino-2-phenylindole (DAPI, blue) staining.
    Superscript Ii Rt Protocol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher superscript ii rnase h
    Runx2 deficient mice have higher levels of BMP-3B. (a) Total <t>RNA</t> from mesenchymal cells of calvarial tissue from wild type and Runx2 -/- (17.5 dpc) was hybridized with osteogenesis-related <t>cDNA</t> array. Signal of BMP-3B on blots is indicated by an arrow. Control genes (GAPDH, cyclophilin A) are shown in lower panel. ( b ) BMP-3B mRNA levels (normalized to GAPDH) in calvarial mesenchymal cells from wild type (WT) and Runx2 -/- animals as detected by qRT-PCR analysis. ( c ) Primary calvarial cells from Runx2 deficient animals were transduced with empty vector (EV) or Runx2 expressing adenovirus with increasing multiplicity of infection (MOI 10 and 20) for 48 hr. Total RNA isolated from the infected cells was utilized to examine BMP-3B mRNA as detected by qRT-PCR analysis. ( d ) Runx2 mRNA expression levels were examined by qRT-PCR and the gene expression levels were normalized to 28S internal control. The gene expression levels were calibrated to IMR-90 cells. ( e ) BMP-3B mRNA expression levels were examined by qRT-PCR and the gene expression levels were normalized to 28S internal control. The BMP-3B gene expression levels were calibrated to Runx2 levels in IMR-90 cells. ( f ) Runx2 protein levels were examined in nuclear extracts of normal lung fibroblast cells (IMR-90 and WI-38) and lung cancer cell lines (H720 and H1299) by immunoblotting with Runx2 monoclonal antibody. Runx2 expression levels were normalized to internal control LaminA/C protein. ( g ) Runx2 intracellular localization was determined by immunofluorescence of endogenous Runx2 protein in WI-38 and H1299 cells. The punctate (Alexa 488, green) signal shows Runx2 staining while nuclei are revealed by 4, 6-diamidino-2-phenylindole (DAPI, blue) staining.
    Superscript Ii Rnase H, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 493 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    GE Healthcare superscript ii
    Runx2 deficient mice have higher levels of BMP-3B. (a) Total <t>RNA</t> from mesenchymal cells of calvarial tissue from wild type and Runx2 -/- (17.5 dpc) was hybridized with osteogenesis-related <t>cDNA</t> array. Signal of BMP-3B on blots is indicated by an arrow. Control genes (GAPDH, cyclophilin A) are shown in lower panel. ( b ) BMP-3B mRNA levels (normalized to GAPDH) in calvarial mesenchymal cells from wild type (WT) and Runx2 -/- animals as detected by qRT-PCR analysis. ( c ) Primary calvarial cells from Runx2 deficient animals were transduced with empty vector (EV) or Runx2 expressing adenovirus with increasing multiplicity of infection (MOI 10 and 20) for 48 hr. Total RNA isolated from the infected cells was utilized to examine BMP-3B mRNA as detected by qRT-PCR analysis. ( d ) Runx2 mRNA expression levels were examined by qRT-PCR and the gene expression levels were normalized to 28S internal control. The gene expression levels were calibrated to IMR-90 cells. ( e ) BMP-3B mRNA expression levels were examined by qRT-PCR and the gene expression levels were normalized to 28S internal control. The BMP-3B gene expression levels were calibrated to Runx2 levels in IMR-90 cells. ( f ) Runx2 protein levels were examined in nuclear extracts of normal lung fibroblast cells (IMR-90 and WI-38) and lung cancer cell lines (H720 and H1299) by immunoblotting with Runx2 monoclonal antibody. Runx2 expression levels were normalized to internal control LaminA/C protein. ( g ) Runx2 intracellular localization was determined by immunofluorescence of endogenous Runx2 protein in WI-38 and H1299 cells. The punctate (Alexa 488, green) signal shows Runx2 staining while nuclei are revealed by 4, 6-diamidino-2-phenylindole (DAPI, blue) staining.
    Superscript Ii, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 84/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Fisher Scientific superscript ii
    Runx2 deficient mice have higher levels of BMP-3B. (a) Total <t>RNA</t> from mesenchymal cells of calvarial tissue from wild type and Runx2 -/- (17.5 dpc) was hybridized with osteogenesis-related <t>cDNA</t> array. Signal of BMP-3B on blots is indicated by an arrow. Control genes (GAPDH, cyclophilin A) are shown in lower panel. ( b ) BMP-3B mRNA levels (normalized to GAPDH) in calvarial mesenchymal cells from wild type (WT) and Runx2 -/- animals as detected by qRT-PCR analysis. ( c ) Primary calvarial cells from Runx2 deficient animals were transduced with empty vector (EV) or Runx2 expressing adenovirus with increasing multiplicity of infection (MOI 10 and 20) for 48 hr. Total RNA isolated from the infected cells was utilized to examine BMP-3B mRNA as detected by qRT-PCR analysis. ( d ) Runx2 mRNA expression levels were examined by qRT-PCR and the gene expression levels were normalized to 28S internal control. The gene expression levels were calibrated to IMR-90 cells. ( e ) BMP-3B mRNA expression levels were examined by qRT-PCR and the gene expression levels were normalized to 28S internal control. The BMP-3B gene expression levels were calibrated to Runx2 levels in IMR-90 cells. ( f ) Runx2 protein levels were examined in nuclear extracts of normal lung fibroblast cells (IMR-90 and WI-38) and lung cancer cell lines (H720 and H1299) by immunoblotting with Runx2 monoclonal antibody. Runx2 expression levels were normalized to internal control LaminA/C protein. ( g ) Runx2 intracellular localization was determined by immunofluorescence of endogenous Runx2 protein in WI-38 and H1299 cells. The punctate (Alexa 488, green) signal shows Runx2 staining while nuclei are revealed by 4, 6-diamidino-2-phenylindole (DAPI, blue) staining.
    Superscript Ii, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Thermo Fisher superscript ii reverse transcriptase kit
    Runx2 deficient mice have higher levels of BMP-3B. (a) Total <t>RNA</t> from mesenchymal cells of calvarial tissue from wild type and Runx2 -/- (17.5 dpc) was hybridized with osteogenesis-related <t>cDNA</t> array. Signal of BMP-3B on blots is indicated by an arrow. Control genes (GAPDH, cyclophilin A) are shown in lower panel. ( b ) BMP-3B mRNA levels (normalized to GAPDH) in calvarial mesenchymal cells from wild type (WT) and Runx2 -/- animals as detected by qRT-PCR analysis. ( c ) Primary calvarial cells from Runx2 deficient animals were transduced with empty vector (EV) or Runx2 expressing adenovirus with increasing multiplicity of infection (MOI 10 and 20) for 48 hr. Total RNA isolated from the infected cells was utilized to examine BMP-3B mRNA as detected by qRT-PCR analysis. ( d ) Runx2 mRNA expression levels were examined by qRT-PCR and the gene expression levels were normalized to 28S internal control. The gene expression levels were calibrated to IMR-90 cells. ( e ) BMP-3B mRNA expression levels were examined by qRT-PCR and the gene expression levels were normalized to 28S internal control. The BMP-3B gene expression levels were calibrated to Runx2 levels in IMR-90 cells. ( f ) Runx2 protein levels were examined in nuclear extracts of normal lung fibroblast cells (IMR-90 and WI-38) and lung cancer cell lines (H720 and H1299) by immunoblotting with Runx2 monoclonal antibody. Runx2 expression levels were normalized to internal control LaminA/C protein. ( g ) Runx2 intracellular localization was determined by immunofluorescence of endogenous Runx2 protein in WI-38 and H1299 cells. The punctate (Alexa 488, green) signal shows Runx2 staining while nuclei are revealed by 4, 6-diamidino-2-phenylindole (DAPI, blue) staining.
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    Tissue distribution of mouse CARK gene. Total RNAs were extracted from different mouse tissues using Trizol reagent. Reverse transcribed cDNA including liver, spleen, lung, brain, kidney, skeletal muscle, testis, heart (C57BL/6J), heart (Balb/C) and heart (KM; lanes 1–10) were amplified to give rise to the 261-bp-specific fragment of CARK . The 261 bp band was only detected in mouse heart from three different strains (C57BL/6J, Balb/C and KM). To control for the amount of cDNA in each reaction, a parallel PCR reaction using the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was carried out. RT-PCR products were analyzed on 2% agarose gel.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Mef2c is an essential regulatory element required for unique expression of the cardiac-specific CARK gene

    doi: 10.1111/j.1582-4934.2007.00155.x

    Figure Lengend Snippet: Tissue distribution of mouse CARK gene. Total RNAs were extracted from different mouse tissues using Trizol reagent. Reverse transcribed cDNA including liver, spleen, lung, brain, kidney, skeletal muscle, testis, heart (C57BL/6J), heart (Balb/C) and heart (KM; lanes 1–10) were amplified to give rise to the 261-bp-specific fragment of CARK . The 261 bp band was only detected in mouse heart from three different strains (C57BL/6J, Balb/C and KM). To control for the amount of cDNA in each reaction, a parallel PCR reaction using the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was carried out. RT-PCR products were analyzed on 2% agarose gel.

    Article Snippet: Total RNAs were isolated using Trizol reagent (Invitrogen) and treated with DNase I (Ambion) according to the manufacturer's instructions. cDNAs were generated using oligo(dT)12-18 as a primer and SuperScript II RT-PCR kit (Invitrogen).cDNA was added to 25 μl of reaction volume containing 12.5 μl of 2× SYBR Green Premix EX Taq (TaKaRa, Otsu, Japan), and 400 nmol/L CARK gene-specific primers (forward: 5′-GACAAAGCAGCCAGGGAA; reverse: 5′-AGCAGGCAATCGGAAGAA).

    Techniques: Amplification, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis

    qRT-PCR validation of selected genes using independent biological samples. Predicted fold changes from microarray analysis and relative gene expression fold change from qRT-PCR (y-axis, expression normalized relative to 1.5 month old group, represented by dotted lines at y = 1, −1) for independent biological replicates of rod photoreceptors (n = 4 for each experiment) is shown in (A) and (B), respectively. The same genes were tested on whole retina samples from Nrlp-eGFP mice aged 1.5 and 12 months, n = 4 biological replicates for each time point (C). Error bars indicate ±SEM. Grem2 expression was detected only beginning at 5 months in photoreceptors.

    Journal: PLoS ONE

    Article Title: Distinct Signature of Altered Homeostasis in Aging Rod Photoreceptors: Implications for Retinal Diseases

    doi: 10.1371/journal.pone.0013885

    Figure Lengend Snippet: qRT-PCR validation of selected genes using independent biological samples. Predicted fold changes from microarray analysis and relative gene expression fold change from qRT-PCR (y-axis, expression normalized relative to 1.5 month old group, represented by dotted lines at y = 1, −1) for independent biological replicates of rod photoreceptors (n = 4 for each experiment) is shown in (A) and (B), respectively. The same genes were tested on whole retina samples from Nrlp-eGFP mice aged 1.5 and 12 months, n = 4 biological replicates for each time point (C). Error bars indicate ±SEM. Grem2 expression was detected only beginning at 5 months in photoreceptors.

    Article Snippet: Amplified photoreceptor cDNA was derived using WT-Ovation Pico System (NuGEN), and whole retina cDNA was derived using Superscript II RT-PCR system (Invitrogen). cDNA was used for real-time qRT-PCR using SYBR(R) Green PCR Mastermix (Applied Biosystems, Foster City, CA) on the 7900HT Fast Real-Time PCR System (Applied Biosystems).

    Techniques: Quantitative RT-PCR, Microarray, Expressing, Mouse Assay

    Runx2 deficient mice have higher levels of BMP-3B. (a) Total RNA from mesenchymal cells of calvarial tissue from wild type and Runx2 -/- (17.5 dpc) was hybridized with osteogenesis-related cDNA array. Signal of BMP-3B on blots is indicated by an arrow. Control genes (GAPDH, cyclophilin A) are shown in lower panel. ( b ) BMP-3B mRNA levels (normalized to GAPDH) in calvarial mesenchymal cells from wild type (WT) and Runx2 -/- animals as detected by qRT-PCR analysis. ( c ) Primary calvarial cells from Runx2 deficient animals were transduced with empty vector (EV) or Runx2 expressing adenovirus with increasing multiplicity of infection (MOI 10 and 20) for 48 hr. Total RNA isolated from the infected cells was utilized to examine BMP-3B mRNA as detected by qRT-PCR analysis. ( d ) Runx2 mRNA expression levels were examined by qRT-PCR and the gene expression levels were normalized to 28S internal control. The gene expression levels were calibrated to IMR-90 cells. ( e ) BMP-3B mRNA expression levels were examined by qRT-PCR and the gene expression levels were normalized to 28S internal control. The BMP-3B gene expression levels were calibrated to Runx2 levels in IMR-90 cells. ( f ) Runx2 protein levels were examined in nuclear extracts of normal lung fibroblast cells (IMR-90 and WI-38) and lung cancer cell lines (H720 and H1299) by immunoblotting with Runx2 monoclonal antibody. Runx2 expression levels were normalized to internal control LaminA/C protein. ( g ) Runx2 intracellular localization was determined by immunofluorescence of endogenous Runx2 protein in WI-38 and H1299 cells. The punctate (Alexa 488, green) signal shows Runx2 staining while nuclei are revealed by 4, 6-diamidino-2-phenylindole (DAPI, blue) staining.

    Journal: Molecular Cancer

    Article Title: Runx2 mediates epigenetic silencing of the bone morphogenetic protein-3B (BMP-3B/GDF10) in lung cancer cells

    doi: 10.1186/1476-4598-11-27

    Figure Lengend Snippet: Runx2 deficient mice have higher levels of BMP-3B. (a) Total RNA from mesenchymal cells of calvarial tissue from wild type and Runx2 -/- (17.5 dpc) was hybridized with osteogenesis-related cDNA array. Signal of BMP-3B on blots is indicated by an arrow. Control genes (GAPDH, cyclophilin A) are shown in lower panel. ( b ) BMP-3B mRNA levels (normalized to GAPDH) in calvarial mesenchymal cells from wild type (WT) and Runx2 -/- animals as detected by qRT-PCR analysis. ( c ) Primary calvarial cells from Runx2 deficient animals were transduced with empty vector (EV) or Runx2 expressing adenovirus with increasing multiplicity of infection (MOI 10 and 20) for 48 hr. Total RNA isolated from the infected cells was utilized to examine BMP-3B mRNA as detected by qRT-PCR analysis. ( d ) Runx2 mRNA expression levels were examined by qRT-PCR and the gene expression levels were normalized to 28S internal control. The gene expression levels were calibrated to IMR-90 cells. ( e ) BMP-3B mRNA expression levels were examined by qRT-PCR and the gene expression levels were normalized to 28S internal control. The BMP-3B gene expression levels were calibrated to Runx2 levels in IMR-90 cells. ( f ) Runx2 protein levels were examined in nuclear extracts of normal lung fibroblast cells (IMR-90 and WI-38) and lung cancer cell lines (H720 and H1299) by immunoblotting with Runx2 monoclonal antibody. Runx2 expression levels were normalized to internal control LaminA/C protein. ( g ) Runx2 intracellular localization was determined by immunofluorescence of endogenous Runx2 protein in WI-38 and H1299 cells. The punctate (Alexa 488, green) signal shows Runx2 staining while nuclei are revealed by 4, 6-diamidino-2-phenylindole (DAPI, blue) staining.

    Article Snippet: Purified RNA was oligo dT primed and cDNA synthesized at 42°C with SuperScript II RNA polymerase (Invitrogen).

    Techniques: Mouse Assay, Quantitative RT-PCR, Transduction, Plasmid Preparation, Expressing, Infection, Isolation, Immunofluorescence, Staining