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  • 77
    Thermo Fisher superscript double stranded complementary dna
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    ET-Rs are expressed in cells of oligodendrocyte lineage in culture and in vivo and in progenitors of the subventricular zone (A-F) Immunostaining was performed in cells of oligodendrocyte lineage in culture: 57% of cells were NG2 + , 52% O4 + , and 26% O1 + . Double immunostaining utilized the above-mentioned cell surface antibodies (green) and anti-ET-R antibodies (red). Nuclear staining utilized DAPI (blue). Smaller panels show individual channels. Both receptors are expressed in NG2 + , O4 + , and O1 + cells. Scale bars = 30μm. (G) RT-PCR analysis of ET-Rs mRNA expression in cultured oligodendrocyte lineage cells. Total <t>RNA</t> was extracted from OPCs at 1-5 days in culture. ET A -R mRNA showed time-dependent increase in culture; ET B -R mRNA levels remained constant. (H) Cell lysates from cultured OPCs were analyzed by Western blot at 1-5 days in culture. Expression levels of both ET-R proteins match those of their mRNAs. (I-J) ET-R mRNA expression in EGFP + cells FACS-purified from CNP-EGFP transgenic mouse brain. Total RNA was obtained from EGFP + cells of total brain (GFP) and SVZ. Double sorting from SVZ used an antibody against the NG2 proteoglycan, obtaining a pure population of NG2-expressing EGFP + progenitors (NG2). Total mRNA was reverse transcribed to <t>cDNA</t> and analyzed by PCR. mRNA for both receptors was expressed in brain EGFP + oligodendrocyte lineage cells, in SVZ OPCs, and in NG2-expressing progenitors of SVZ. Note: In NG2-expressing progenitors ET A -R shows lower mRNA expression than ET B -R. (K-L) Immunostaining in dissociated cells from the SVZ of p8 CNP-EGFP mice. Two hours after plating, cells were immunostained with antibodies against NG2 and the ET A (A) or the ET B (B) receptors. 98% of NG2 + EGFP + (green) cells in the SVZ displayed immunostaining for both ET-Rs (red).
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    ET-Rs are expressed in cells of oligodendrocyte lineage in culture and in vivo and in progenitors of the subventricular zone (A-F) Immunostaining was performed in cells of oligodendrocyte lineage in culture: 57% of cells were NG2 + , 52% O4 + , and 26% O1 + . Double immunostaining utilized the above-mentioned cell surface antibodies (green) and anti-ET-R antibodies (red). Nuclear staining utilized DAPI (blue). Smaller panels show individual channels. Both receptors are expressed in NG2 + , O4 + , and O1 + cells. Scale bars = 30μm. (G) RT-PCR analysis of ET-Rs mRNA expression in cultured oligodendrocyte lineage cells. Total <t>RNA</t> was extracted from OPCs at 1-5 days in culture. ET A -R mRNA showed time-dependent increase in culture; ET B -R mRNA levels remained constant. (H) Cell lysates from cultured OPCs were analyzed by Western blot at 1-5 days in culture. Expression levels of both ET-R proteins match those of their mRNAs. (I-J) ET-R mRNA expression in EGFP + cells FACS-purified from CNP-EGFP transgenic mouse brain. Total RNA was obtained from EGFP + cells of total brain (GFP) and SVZ. Double sorting from SVZ used an antibody against the NG2 proteoglycan, obtaining a pure population of NG2-expressing EGFP + progenitors (NG2). Total mRNA was reverse transcribed to <t>cDNA</t> and analyzed by PCR. mRNA for both receptors was expressed in brain EGFP + oligodendrocyte lineage cells, in SVZ OPCs, and in NG2-expressing progenitors of SVZ. Note: In NG2-expressing progenitors ET A -R shows lower mRNA expression than ET B -R. (K-L) Immunostaining in dissociated cells from the SVZ of p8 CNP-EGFP mice. Two hours after plating, cells were immunostained with antibodies against NG2 and the ET A (A) or the ET B (B) receptors. 98% of NG2 + EGFP + (green) cells in the SVZ displayed immunostaining for both ET-Rs (red).
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    ET-Rs are expressed in cells of oligodendrocyte lineage in culture and in vivo and in progenitors of the subventricular zone (A-F) Immunostaining was performed in cells of oligodendrocyte lineage in culture: 57% of cells were NG2 + , 52% O4 + , and 26% O1 + . Double immunostaining utilized the above-mentioned cell surface antibodies (green) and anti-ET-R antibodies (red). Nuclear staining utilized DAPI (blue). Smaller panels show individual channels. Both receptors are expressed in NG2 + , O4 + , and O1 + cells. Scale bars = 30μm. (G) RT-PCR analysis of ET-Rs mRNA expression in cultured oligodendrocyte lineage cells. Total <t>RNA</t> was extracted from OPCs at 1-5 days in culture. ET A -R mRNA showed time-dependent increase in culture; ET B -R mRNA levels remained constant. (H) Cell lysates from cultured OPCs were analyzed by Western blot at 1-5 days in culture. Expression levels of both ET-R proteins match those of their mRNAs. (I-J) ET-R mRNA expression in EGFP + cells FACS-purified from CNP-EGFP transgenic mouse brain. Total RNA was obtained from EGFP + cells of total brain (GFP) and SVZ. Double sorting from SVZ used an antibody against the NG2 proteoglycan, obtaining a pure population of NG2-expressing EGFP + progenitors (NG2). Total mRNA was reverse transcribed to <t>cDNA</t> and analyzed by PCR. mRNA for both receptors was expressed in brain EGFP + oligodendrocyte lineage cells, in SVZ OPCs, and in NG2-expressing progenitors of SVZ. Note: In NG2-expressing progenitors ET A -R shows lower mRNA expression than ET B -R. (K-L) Immunostaining in dissociated cells from the SVZ of p8 CNP-EGFP mice. Two hours after plating, cells were immunostained with antibodies against NG2 and the ET A (A) or the ET B (B) receptors. 98% of NG2 + EGFP + (green) cells in the SVZ displayed immunostaining for both ET-Rs (red).
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    ET-Rs are expressed in cells of oligodendrocyte lineage in culture and in vivo and in progenitors of the subventricular zone (A-F) Immunostaining was performed in cells of oligodendrocyte lineage in culture: 57% of cells were NG2 + , 52% O4 + , and 26% O1 + . Double immunostaining utilized the above-mentioned cell surface antibodies (green) and anti-ET-R antibodies (red). Nuclear staining utilized DAPI (blue). Smaller panels show individual channels. Both receptors are expressed in NG2 + , O4 + , and O1 + cells. Scale bars = 30μm. (G) RT-PCR analysis of ET-Rs mRNA expression in cultured oligodendrocyte lineage cells. Total <t>RNA</t> was extracted from OPCs at 1-5 days in culture. ET A -R mRNA showed time-dependent increase in culture; ET B -R mRNA levels remained constant. (H) Cell lysates from cultured OPCs were analyzed by Western blot at 1-5 days in culture. Expression levels of both ET-R proteins match those of their mRNAs. (I-J) ET-R mRNA expression in EGFP + cells FACS-purified from CNP-EGFP transgenic mouse brain. Total RNA was obtained from EGFP + cells of total brain (GFP) and SVZ. Double sorting from SVZ used an antibody against the NG2 proteoglycan, obtaining a pure population of NG2-expressing EGFP + progenitors (NG2). Total mRNA was reverse transcribed to <t>cDNA</t> and analyzed by PCR. mRNA for both receptors was expressed in brain EGFP + oligodendrocyte lineage cells, in SVZ OPCs, and in NG2-expressing progenitors of SVZ. Note: In NG2-expressing progenitors ET A -R shows lower mRNA expression than ET B -R. (K-L) Immunostaining in dissociated cells from the SVZ of p8 CNP-EGFP mice. Two hours after plating, cells were immunostained with antibodies against NG2 and the ET A (A) or the ET B (B) receptors. 98% of NG2 + EGFP + (green) cells in the SVZ displayed immunostaining for both ET-Rs (red).
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    ET-Rs are expressed in cells of oligodendrocyte lineage in culture and in vivo and in progenitors of the subventricular zone (A-F) Immunostaining was performed in cells of oligodendrocyte lineage in culture: 57% of cells were NG2 + , 52% O4 + , and 26% O1 + . Double immunostaining utilized the above-mentioned cell surface antibodies (green) and anti-ET-R antibodies (red). Nuclear staining utilized DAPI (blue). Smaller panels show individual channels. Both receptors are expressed in NG2 + , O4 + , and O1 + cells. Scale bars = 30μm. (G) RT-PCR analysis of ET-Rs mRNA expression in cultured oligodendrocyte lineage cells. Total <t>RNA</t> was extracted from OPCs at 1-5 days in culture. ET A -R mRNA showed time-dependent increase in culture; ET B -R mRNA levels remained constant. (H) Cell lysates from cultured OPCs were analyzed by Western blot at 1-5 days in culture. Expression levels of both ET-R proteins match those of their mRNAs. (I-J) ET-R mRNA expression in EGFP + cells FACS-purified from CNP-EGFP transgenic mouse brain. Total RNA was obtained from EGFP + cells of total brain (GFP) and SVZ. Double sorting from SVZ used an antibody against the NG2 proteoglycan, obtaining a pure population of NG2-expressing EGFP + progenitors (NG2). Total mRNA was reverse transcribed to <t>cDNA</t> and analyzed by PCR. mRNA for both receptors was expressed in brain EGFP + oligodendrocyte lineage cells, in SVZ OPCs, and in NG2-expressing progenitors of SVZ. Note: In NG2-expressing progenitors ET A -R shows lower mRNA expression than ET B -R. (K-L) Immunostaining in dissociated cells from the SVZ of p8 CNP-EGFP mice. Two hours after plating, cells were immunostained with antibodies against NG2 and the ET A (A) or the ET B (B) receptors. 98% of NG2 + EGFP + (green) cells in the SVZ displayed immunostaining for both ET-Rs (red).
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    ET-Rs are expressed in cells of oligodendrocyte lineage in culture and in vivo and in progenitors of the subventricular zone (A-F) Immunostaining was performed in cells of oligodendrocyte lineage in culture: 57% of cells were NG2 + , 52% O4 + , and 26% O1 + . Double immunostaining utilized the above-mentioned cell surface antibodies (green) and anti-ET-R antibodies (red). Nuclear staining utilized DAPI (blue). Smaller panels show individual channels. Both receptors are expressed in NG2 + , O4 + , and O1 + cells. Scale bars = 30μm. (G) RT-PCR analysis of ET-Rs mRNA expression in cultured oligodendrocyte lineage cells. Total <t>RNA</t> was extracted from OPCs at 1-5 days in culture. ET A -R mRNA showed time-dependent increase in culture; ET B -R mRNA levels remained constant. (H) Cell lysates from cultured OPCs were analyzed by Western blot at 1-5 days in culture. Expression levels of both ET-R proteins match those of their mRNAs. (I-J) ET-R mRNA expression in EGFP + cells FACS-purified from CNP-EGFP transgenic mouse brain. Total RNA was obtained from EGFP + cells of total brain (GFP) and SVZ. Double sorting from SVZ used an antibody against the NG2 proteoglycan, obtaining a pure population of NG2-expressing EGFP + progenitors (NG2). Total mRNA was reverse transcribed to <t>cDNA</t> and analyzed by PCR. mRNA for both receptors was expressed in brain EGFP + oligodendrocyte lineage cells, in SVZ OPCs, and in NG2-expressing progenitors of SVZ. Note: In NG2-expressing progenitors ET A -R shows lower mRNA expression than ET B -R. (K-L) Immunostaining in dissociated cells from the SVZ of p8 CNP-EGFP mice. Two hours after plating, cells were immunostained with antibodies against NG2 and the ET A (A) or the ET B (B) receptors. 98% of NG2 + EGFP + (green) cells in the SVZ displayed immunostaining for both ET-Rs (red).
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    ET-Rs are expressed in cells of oligodendrocyte lineage in culture and in vivo and in progenitors of the subventricular zone (A-F) Immunostaining was performed in cells of oligodendrocyte lineage in culture: 57% of cells were NG2 + , 52% O4 + , and 26% O1 + . Double immunostaining utilized the above-mentioned cell surface antibodies (green) and anti-ET-R antibodies (red). Nuclear staining utilized DAPI (blue). Smaller panels show individual channels. Both receptors are expressed in NG2 + , O4 + , and O1 + cells. Scale bars = 30μm. (G) RT-PCR analysis of ET-Rs mRNA expression in cultured oligodendrocyte lineage cells. Total <t>RNA</t> was extracted from OPCs at 1-5 days in culture. ET A -R mRNA showed time-dependent increase in culture; ET B -R mRNA levels remained constant. (H) Cell lysates from cultured OPCs were analyzed by Western blot at 1-5 days in culture. Expression levels of both ET-R proteins match those of their mRNAs. (I-J) ET-R mRNA expression in EGFP + cells FACS-purified from CNP-EGFP transgenic mouse brain. Total RNA was obtained from EGFP + cells of total brain (GFP) and SVZ. Double sorting from SVZ used an antibody against the NG2 proteoglycan, obtaining a pure population of NG2-expressing EGFP + progenitors (NG2). Total mRNA was reverse transcribed to <t>cDNA</t> and analyzed by PCR. mRNA for both receptors was expressed in brain EGFP + oligodendrocyte lineage cells, in SVZ OPCs, and in NG2-expressing progenitors of SVZ. Note: In NG2-expressing progenitors ET A -R shows lower mRNA expression than ET B -R. (K-L) Immunostaining in dissociated cells from the SVZ of p8 CNP-EGFP mice. Two hours after plating, cells were immunostained with antibodies against NG2 and the ET A (A) or the ET B (B) receptors. 98% of NG2 + EGFP + (green) cells in the SVZ displayed immunostaining for both ET-Rs (red).
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    ET-Rs are expressed in cells of oligodendrocyte lineage in culture and in vivo and in progenitors of the subventricular zone (A-F) Immunostaining was performed in cells of oligodendrocyte lineage in culture: 57% of cells were NG2 + , 52% O4 + , and 26% O1 + . Double immunostaining utilized the above-mentioned cell surface antibodies (green) and anti-ET-R antibodies (red). Nuclear staining utilized DAPI (blue). Smaller panels show individual channels. Both receptors are expressed in NG2 + , O4 + , and O1 + cells. Scale bars = 30μm. (G) RT-PCR analysis of ET-Rs mRNA expression in cultured oligodendrocyte lineage cells. Total <t>RNA</t> was extracted from OPCs at 1-5 days in culture. ET A -R mRNA showed time-dependent increase in culture; ET B -R mRNA levels remained constant. (H) Cell lysates from cultured OPCs were analyzed by Western blot at 1-5 days in culture. Expression levels of both ET-R proteins match those of their mRNAs. (I-J) ET-R mRNA expression in EGFP + cells FACS-purified from CNP-EGFP transgenic mouse brain. Total RNA was obtained from EGFP + cells of total brain (GFP) and SVZ. Double sorting from SVZ used an antibody against the NG2 proteoglycan, obtaining a pure population of NG2-expressing EGFP + progenitors (NG2). Total mRNA was reverse transcribed to <t>cDNA</t> and analyzed by PCR. mRNA for both receptors was expressed in brain EGFP + oligodendrocyte lineage cells, in SVZ OPCs, and in NG2-expressing progenitors of SVZ. Note: In NG2-expressing progenitors ET A -R shows lower mRNA expression than ET B -R. (K-L) Immunostaining in dissociated cells from the SVZ of p8 CNP-EGFP mice. Two hours after plating, cells were immunostained with antibodies against NG2 and the ET A (A) or the ET B (B) receptors. 98% of NG2 + EGFP + (green) cells in the SVZ displayed immunostaining for both ET-Rs (red).
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    ET-Rs are expressed in cells of oligodendrocyte lineage in culture and in vivo and in progenitors of the subventricular zone (A-F) Immunostaining was performed in cells of oligodendrocyte lineage in culture: 57% of cells were NG2 + , 52% O4 + , and 26% O1 + . Double immunostaining utilized the above-mentioned cell surface antibodies (green) and anti-ET-R antibodies (red). Nuclear staining utilized DAPI (blue). Smaller panels show individual channels. Both receptors are expressed in NG2 + , O4 + , and O1 + cells. Scale bars = 30μm. (G) RT-PCR analysis of ET-Rs mRNA expression in cultured oligodendrocyte lineage cells. Total <t>RNA</t> was extracted from OPCs at 1-5 days in culture. ET A -R mRNA showed time-dependent increase in culture; ET B -R mRNA levels remained constant. (H) Cell lysates from cultured OPCs were analyzed by Western blot at 1-5 days in culture. Expression levels of both ET-R proteins match those of their mRNAs. (I-J) ET-R mRNA expression in EGFP + cells FACS-purified from CNP-EGFP transgenic mouse brain. Total RNA was obtained from EGFP + cells of total brain (GFP) and SVZ. Double sorting from SVZ used an antibody against the NG2 proteoglycan, obtaining a pure population of NG2-expressing EGFP + progenitors (NG2). Total mRNA was reverse transcribed to <t>cDNA</t> and analyzed by PCR. mRNA for both receptors was expressed in brain EGFP + oligodendrocyte lineage cells, in SVZ OPCs, and in NG2-expressing progenitors of SVZ. Note: In NG2-expressing progenitors ET A -R shows lower mRNA expression than ET B -R. (K-L) Immunostaining in dissociated cells from the SVZ of p8 CNP-EGFP mice. Two hours after plating, cells were immunostained with antibodies against NG2 and the ET A (A) or the ET B (B) receptors. 98% of NG2 + EGFP + (green) cells in the SVZ displayed immunostaining for both ET-Rs (red).
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    Image Search Results


    ET-Rs are expressed in cells of oligodendrocyte lineage in culture and in vivo and in progenitors of the subventricular zone (A-F) Immunostaining was performed in cells of oligodendrocyte lineage in culture: 57% of cells were NG2 + , 52% O4 + , and 26% O1 + . Double immunostaining utilized the above-mentioned cell surface antibodies (green) and anti-ET-R antibodies (red). Nuclear staining utilized DAPI (blue). Smaller panels show individual channels. Both receptors are expressed in NG2 + , O4 + , and O1 + cells. Scale bars = 30μm. (G) RT-PCR analysis of ET-Rs mRNA expression in cultured oligodendrocyte lineage cells. Total RNA was extracted from OPCs at 1-5 days in culture. ET A -R mRNA showed time-dependent increase in culture; ET B -R mRNA levels remained constant. (H) Cell lysates from cultured OPCs were analyzed by Western blot at 1-5 days in culture. Expression levels of both ET-R proteins match those of their mRNAs. (I-J) ET-R mRNA expression in EGFP + cells FACS-purified from CNP-EGFP transgenic mouse brain. Total RNA was obtained from EGFP + cells of total brain (GFP) and SVZ. Double sorting from SVZ used an antibody against the NG2 proteoglycan, obtaining a pure population of NG2-expressing EGFP + progenitors (NG2). Total mRNA was reverse transcribed to cDNA and analyzed by PCR. mRNA for both receptors was expressed in brain EGFP + oligodendrocyte lineage cells, in SVZ OPCs, and in NG2-expressing progenitors of SVZ. Note: In NG2-expressing progenitors ET A -R shows lower mRNA expression than ET B -R. (K-L) Immunostaining in dissociated cells from the SVZ of p8 CNP-EGFP mice. Two hours after plating, cells were immunostained with antibodies against NG2 and the ET A (A) or the ET B (B) receptors. 98% of NG2 + EGFP + (green) cells in the SVZ displayed immunostaining for both ET-Rs (red).

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Endothelin-1 regulates oligodendrocyte development

    doi: 10.1523/JNEUROSCI.0822-09.2009

    Figure Lengend Snippet: ET-Rs are expressed in cells of oligodendrocyte lineage in culture and in vivo and in progenitors of the subventricular zone (A-F) Immunostaining was performed in cells of oligodendrocyte lineage in culture: 57% of cells were NG2 + , 52% O4 + , and 26% O1 + . Double immunostaining utilized the above-mentioned cell surface antibodies (green) and anti-ET-R antibodies (red). Nuclear staining utilized DAPI (blue). Smaller panels show individual channels. Both receptors are expressed in NG2 + , O4 + , and O1 + cells. Scale bars = 30μm. (G) RT-PCR analysis of ET-Rs mRNA expression in cultured oligodendrocyte lineage cells. Total RNA was extracted from OPCs at 1-5 days in culture. ET A -R mRNA showed time-dependent increase in culture; ET B -R mRNA levels remained constant. (H) Cell lysates from cultured OPCs were analyzed by Western blot at 1-5 days in culture. Expression levels of both ET-R proteins match those of their mRNAs. (I-J) ET-R mRNA expression in EGFP + cells FACS-purified from CNP-EGFP transgenic mouse brain. Total RNA was obtained from EGFP + cells of total brain (GFP) and SVZ. Double sorting from SVZ used an antibody against the NG2 proteoglycan, obtaining a pure population of NG2-expressing EGFP + progenitors (NG2). Total mRNA was reverse transcribed to cDNA and analyzed by PCR. mRNA for both receptors was expressed in brain EGFP + oligodendrocyte lineage cells, in SVZ OPCs, and in NG2-expressing progenitors of SVZ. Note: In NG2-expressing progenitors ET A -R shows lower mRNA expression than ET B -R. (K-L) Immunostaining in dissociated cells from the SVZ of p8 CNP-EGFP mice. Two hours after plating, cells were immunostained with antibodies against NG2 and the ET A (A) or the ET B (B) receptors. 98% of NG2 + EGFP + (green) cells in the SVZ displayed immunostaining for both ET-Rs (red).

    Article Snippet: 1 μg RNA from each sample was reverse-transcribed using the SuperScript™ First-Strand cDNA Synthesis kit (Invitrogen).

    Techniques: In Vivo, Immunostaining, Double Immunostaining, Staining, Reverse Transcription Polymerase Chain Reaction, Expressing, Cell Culture, Western Blot, FACS, Purification, Transgenic Assay, Polymerase Chain Reaction, Mouse Assay