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  • 99
    Qiagen superfect
    miR-218 regulates DCC expression. (A) Immunofluorescence and Western blot experiments showing endogenous DCC expression in the IMR-32 cell line. Numbers on top of the DCC bands represent micrograms of protein loaded. Scale bar= 50μm. (B) Significant decreased of DCC mRNA expression in IMR-32 cells 24h after <t>transfection</t> with a miR-218 mimic (n=3) in comparison to transfection with reagent alone (mock; n=3) or mimic scramble (miR-Scr; n=3) (F (2,6) =11.75; p =0.008; *different from mock; †different from miR-Scr, *†p
    Superfect, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 11267 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superfect/product/Qiagen
    Average 99 stars, based on 11267 article reviews
    Price from $9.99 to $1999.99
    superfect - by Bioz Stars, 2021-02
    99/100 stars
      Buy from Supplier

    92
    Thermo Fisher superfect
    miR-218 regulates DCC expression. (A) Immunofluorescence and Western blot experiments showing endogenous DCC expression in the IMR-32 cell line. Numbers on top of the DCC bands represent micrograms of protein loaded. Scale bar= 50μm. (B) Significant decreased of DCC mRNA expression in IMR-32 cells 24h after <t>transfection</t> with a miR-218 mimic (n=3) in comparison to transfection with reagent alone (mock; n=3) or mimic scramble (miR-Scr; n=3) (F (2,6) =11.75; p =0.008; *different from mock; †different from miR-Scr, *†p
    Superfect, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 220 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superfect/product/Thermo Fisher
    Average 92 stars, based on 220 article reviews
    Price from $9.99 to $1999.99
    superfect - by Bioz Stars, 2021-02
    92/100 stars
      Buy from Supplier

    97
    Thermo Fisher superfect transfection reagent
    miR-218 regulates DCC expression. (A) Immunofluorescence and Western blot experiments showing endogenous DCC expression in the IMR-32 cell line. Numbers on top of the DCC bands represent micrograms of protein loaded. Scale bar= 50μm. (B) Significant decreased of DCC mRNA expression in IMR-32 cells 24h after <t>transfection</t> with a miR-218 mimic (n=3) in comparison to transfection with reagent alone (mock; n=3) or mimic scramble (miR-Scr; n=3) (F (2,6) =11.75; p =0.008; *different from mock; †different from miR-Scr, *†p
    Superfect Transfection Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 236 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superfect transfection reagent/product/Thermo Fisher
    Average 97 stars, based on 236 article reviews
    Price from $9.99 to $1999.99
    superfect transfection reagent - by Bioz Stars, 2021-02
    97/100 stars
      Buy from Supplier

    97
    Qiagen superfect kit
    miR-218 regulates DCC expression. (A) Immunofluorescence and Western blot experiments showing endogenous DCC expression in the IMR-32 cell line. Numbers on top of the DCC bands represent micrograms of protein loaded. Scale bar= 50μm. (B) Significant decreased of DCC mRNA expression in IMR-32 cells 24h after <t>transfection</t> with a miR-218 mimic (n=3) in comparison to transfection with reagent alone (mock; n=3) or mimic scramble (miR-Scr; n=3) (F (2,6) =11.75; p =0.008; *different from mock; †different from miR-Scr, *†p
    Superfect Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superfect kit/product/Qiagen
    Average 97 stars, based on 57 article reviews
    Price from $9.99 to $1999.99
    superfect kit - by Bioz Stars, 2021-02
    97/100 stars
      Buy from Supplier

    Image Search Results


    miR-218 regulates DCC expression. (A) Immunofluorescence and Western blot experiments showing endogenous DCC expression in the IMR-32 cell line. Numbers on top of the DCC bands represent micrograms of protein loaded. Scale bar= 50μm. (B) Significant decreased of DCC mRNA expression in IMR-32 cells 24h after transfection with a miR-218 mimic (n=3) in comparison to transfection with reagent alone (mock; n=3) or mimic scramble (miR-Scr; n=3) (F (2,6) =11.75; p =0.008; *different from mock; †different from miR-Scr, *†p

    Journal: Biological psychiatry

    Article Title: DCC confers susceptibility to depression-like behaviors in humans and mice and is regulated by miR-218

    doi: 10.1016/j.biopsych.2016.08.017

    Figure Lengend Snippet: miR-218 regulates DCC expression. (A) Immunofluorescence and Western blot experiments showing endogenous DCC expression in the IMR-32 cell line. Numbers on top of the DCC bands represent micrograms of protein loaded. Scale bar= 50μm. (B) Significant decreased of DCC mRNA expression in IMR-32 cells 24h after transfection with a miR-218 mimic (n=3) in comparison to transfection with reagent alone (mock; n=3) or mimic scramble (miR-Scr; n=3) (F (2,6) =11.75; p =0.008; *different from mock; †different from miR-Scr, *†p

    Article Snippet: A total of 4x105 cells were grown for 24hr with transfection reagent alone, or transfection reagent in combination of the miR-218 mimic and two target protectors, according to the manufacturer’s protocol (Qiagen, Toronto, ON, Canada).

    Techniques: Droplet Countercurrent Chromatography, Expressing, Immunofluorescence, Western Blot, Transfection