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  • 95
    ATCC subclone 14
    Subclone 14, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/subclone 14/product/ATCC
    Average 95 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    subclone 14 - by Bioz Stars, 2020-07
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    96
    Thermo Fisher subcloning efficiency dh5α
    Subcloning Efficiency Dh5α, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/subcloning efficiency dh5α/product/Thermo Fisher
    Average 96 stars, based on 77 article reviews
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    subcloning efficiency dh5α - by Bioz Stars, 2020-07
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    94
    Promega subcloning
    Subcloning, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 606 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 606 article reviews
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    subcloning - by Bioz Stars, 2020-07
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    92
    Thermo Fisher subcloning
    Subcloning, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1596 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/subcloning/product/Thermo Fisher
    Average 92 stars, based on 1596 article reviews
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    93
    Addgene inc subcloning zipacr
    Subcloning Zipacr, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/subcloning zipacr/product/Addgene inc
    Average 93 stars, based on 8 article reviews
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    subcloning zipacr - by Bioz Stars, 2020-07
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    85
    Promega ta subcloning
    Ta Subcloning, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ta subcloning/product/Promega
    Average 85 stars, based on 28 article reviews
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    ta subcloning - by Bioz Stars, 2020-07
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    91
    Promega subcloning notebook
    Subcloning Notebook, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/subcloning notebook/product/Promega
    Average 91 stars, based on 4 article reviews
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    93
    Oxford Expression Technologies Ltd sf9 subclone
    SDS-PAGE and Western blot analysis of final purified Pfs230C1. (A) SDS-PAGE under non-reducing and reducing conditions followed by silver stain. (B) Western blot with anti-his antibody. (A–B) Lanes 1–2 representative Pfs230C1 from the original Super <t>Sf9</t> process [ 16 ], and Lanes 3–4 Pfs230C1 derived from 20 L optimized process. Non-reducing (Lanes 1, 4) and reducing (Lanes 2, 3) conditions. Western blot analysis of (C) Pfs230C1 and (D) native parasite extracts with mouse monoclonal antibody 15A4-1B12 under reducing (R) and non-reducing (NR) conditions. Different molecular weight marker standards are utilized for panels C and D given the difference in molecular weight of target protein. Expected molecular weight of native Pfs230 is ∼363 kDa, however, since protease inhibitors are not utilized, multiple bands are visible.
    Sf9 Subclone, supplied by Oxford Expression Technologies Ltd, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sf9 subclone/product/Oxford Expression Technologies Ltd
    Average 93 stars, based on 5 article reviews
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    sf9 subclone - by Bioz Stars, 2020-07
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    99
    ATCC subclone 4
    SDS-PAGE and Western blot analysis of final purified Pfs230C1. (A) SDS-PAGE under non-reducing and reducing conditions followed by silver stain. (B) Western blot with anti-his antibody. (A–B) Lanes 1–2 representative Pfs230C1 from the original Super <t>Sf9</t> process [ 16 ], and Lanes 3–4 Pfs230C1 derived from 20 L optimized process. Non-reducing (Lanes 1, 4) and reducing (Lanes 2, 3) conditions. Western blot analysis of (C) Pfs230C1 and (D) native parasite extracts with mouse monoclonal antibody 15A4-1B12 under reducing (R) and non-reducing (NR) conditions. Different molecular weight marker standards are utilized for panels C and D given the difference in molecular weight of target protein. Expected molecular weight of native Pfs230 is ∼363 kDa, however, since protease inhibitors are not utilized, multiple bands are visible.
    Subclone 4, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/subclone 4/product/ATCC
    Average 99 stars, based on 63 article reviews
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    99
    ATCC subclone 24
    SDS-PAGE and Western blot analysis of final purified Pfs230C1. (A) SDS-PAGE under non-reducing and reducing conditions followed by silver stain. (B) Western blot with anti-his antibody. (A–B) Lanes 1–2 representative Pfs230C1 from the original Super <t>Sf9</t> process [ 16 ], and Lanes 3–4 Pfs230C1 derived from 20 L optimized process. Non-reducing (Lanes 1, 4) and reducing (Lanes 2, 3) conditions. Western blot analysis of (C) Pfs230C1 and (D) native parasite extracts with mouse monoclonal antibody 15A4-1B12 under reducing (R) and non-reducing (NR) conditions. Different molecular weight marker standards are utilized for panels C and D given the difference in molecular weight of target protein. Expected molecular weight of native Pfs230 is ∼363 kDa, however, since protease inhibitors are not utilized, multiple bands are visible.
    Subclone 24, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/subclone 24/product/ATCC
    Average 99 stars, based on 1 article reviews
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    subclone 24 - by Bioz Stars, 2020-07
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    85
    Thermo Fisher ligase free subcloning
    SDS-PAGE and Western blot analysis of final purified Pfs230C1. (A) SDS-PAGE under non-reducing and reducing conditions followed by silver stain. (B) Western blot with anti-his antibody. (A–B) Lanes 1–2 representative Pfs230C1 from the original Super <t>Sf9</t> process [ 16 ], and Lanes 3–4 Pfs230C1 derived from 20 L optimized process. Non-reducing (Lanes 1, 4) and reducing (Lanes 2, 3) conditions. Western blot analysis of (C) Pfs230C1 and (D) native parasite extracts with mouse monoclonal antibody 15A4-1B12 under reducing (R) and non-reducing (NR) conditions. Different molecular weight marker standards are utilized for panels C and D given the difference in molecular weight of target protein. Expected molecular weight of native Pfs230 is ∼363 kDa, however, since protease inhibitors are not utilized, multiple bands are visible.
    Ligase Free Subcloning, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ligase free subcloning/product/Thermo Fisher
    Average 85 stars, based on 16 article reviews
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    ligase free subcloning - by Bioz Stars, 2020-07
    85/100 stars
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    85
    ATCC mc3t3 e1 subclone c14
    SDS-PAGE and Western blot analysis of final purified Pfs230C1. (A) SDS-PAGE under non-reducing and reducing conditions followed by silver stain. (B) Western blot with anti-his antibody. (A–B) Lanes 1–2 representative Pfs230C1 from the original Super <t>Sf9</t> process [ 16 ], and Lanes 3–4 Pfs230C1 derived from 20 L optimized process. Non-reducing (Lanes 1, 4) and reducing (Lanes 2, 3) conditions. Western blot analysis of (C) Pfs230C1 and (D) native parasite extracts with mouse monoclonal antibody 15A4-1B12 under reducing (R) and non-reducing (NR) conditions. Different molecular weight marker standards are utilized for panels C and D given the difference in molecular weight of target protein. Expected molecular weight of native Pfs230 is ∼363 kDa, however, since protease inhibitors are not utilized, multiple bands are visible.
    Mc3t3 E1 Subclone C14, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mc3t3 e1 subclone c14/product/ATCC
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    mc3t3 e1 subclone c14 - by Bioz Stars, 2020-07
    85/100 stars
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    85
    Thermo Fisher subcloning eyfp
    SDS-PAGE and Western blot analysis of final purified Pfs230C1. (A) SDS-PAGE under non-reducing and reducing conditions followed by silver stain. (B) Western blot with anti-his antibody. (A–B) Lanes 1–2 representative Pfs230C1 from the original Super <t>Sf9</t> process [ 16 ], and Lanes 3–4 Pfs230C1 derived from 20 L optimized process. Non-reducing (Lanes 1, 4) and reducing (Lanes 2, 3) conditions. Western blot analysis of (C) Pfs230C1 and (D) native parasite extracts with mouse monoclonal antibody 15A4-1B12 under reducing (R) and non-reducing (NR) conditions. Different molecular weight marker standards are utilized for panels C and D given the difference in molecular weight of target protein. Expected molecular weight of native Pfs230 is ∼363 kDa, however, since protease inhibitors are not utilized, multiple bands are visible.
    Subcloning Eyfp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 9 article reviews
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    subcloning eyfp - by Bioz Stars, 2020-07
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    99
    Qiagen subcloning kit
    SDS-PAGE and Western blot analysis of final purified Pfs230C1. (A) SDS-PAGE under non-reducing and reducing conditions followed by silver stain. (B) Western blot with anti-his antibody. (A–B) Lanes 1–2 representative Pfs230C1 from the original Super <t>Sf9</t> process [ 16 ], and Lanes 3–4 Pfs230C1 derived from 20 L optimized process. Non-reducing (Lanes 1, 4) and reducing (Lanes 2, 3) conditions. Western blot analysis of (C) Pfs230C1 and (D) native parasite extracts with mouse monoclonal antibody 15A4-1B12 under reducing (R) and non-reducing (NR) conditions. Different molecular weight marker standards are utilized for panels C and D given the difference in molecular weight of target protein. Expected molecular weight of native Pfs230 is ∼363 kDa, however, since protease inhibitors are not utilized, multiple bands are visible.
    Subcloning Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/subcloning kit/product/Qiagen
    Average 99 stars, based on 21 article reviews
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    subcloning kit - by Bioz Stars, 2020-07
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    91
    Thermo Fisher subcloning puno1 hcd200
    SDS-PAGE and Western blot analysis of final purified Pfs230C1. (A) SDS-PAGE under non-reducing and reducing conditions followed by silver stain. (B) Western blot with anti-his antibody. (A–B) Lanes 1–2 representative Pfs230C1 from the original Super <t>Sf9</t> process [ 16 ], and Lanes 3–4 Pfs230C1 derived from 20 L optimized process. Non-reducing (Lanes 1, 4) and reducing (Lanes 2, 3) conditions. Western blot analysis of (C) Pfs230C1 and (D) native parasite extracts with mouse monoclonal antibody 15A4-1B12 under reducing (R) and non-reducing (NR) conditions. Different molecular weight marker standards are utilized for panels C and D given the difference in molecular weight of target protein. Expected molecular weight of native Pfs230 is ∼363 kDa, however, since protease inhibitors are not utilized, multiple bands are visible.
    Subcloning Puno1 Hcd200, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/subcloning puno1 hcd200/product/Thermo Fisher
    Average 91 stars, based on 10 article reviews
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    subcloning puno1 hcd200 - by Bioz Stars, 2020-07
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    93
    Thermo Fisher gateway subcloning system
    SDS-PAGE and Western blot analysis of final purified Pfs230C1. (A) SDS-PAGE under non-reducing and reducing conditions followed by silver stain. (B) Western blot with anti-his antibody. (A–B) Lanes 1–2 representative Pfs230C1 from the original Super <t>Sf9</t> process [ 16 ], and Lanes 3–4 Pfs230C1 derived from 20 L optimized process. Non-reducing (Lanes 1, 4) and reducing (Lanes 2, 3) conditions. Western blot analysis of (C) Pfs230C1 and (D) native parasite extracts with mouse monoclonal antibody 15A4-1B12 under reducing (R) and non-reducing (NR) conditions. Different molecular weight marker standards are utilized for panels C and D given the difference in molecular weight of target protein. Expected molecular weight of native Pfs230 is ∼363 kDa, however, since protease inhibitors are not utilized, multiple bands are visible.
    Gateway Subcloning System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gateway subcloning system/product/Thermo Fisher
    Average 93 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    gateway subcloning system - by Bioz Stars, 2020-07
    93/100 stars
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    89
    Thermo Fisher topo subcloning
    SDS-PAGE and Western blot analysis of final purified Pfs230C1. (A) SDS-PAGE under non-reducing and reducing conditions followed by silver stain. (B) Western blot with anti-his antibody. (A–B) Lanes 1–2 representative Pfs230C1 from the original Super <t>Sf9</t> process [ 16 ], and Lanes 3–4 Pfs230C1 derived from 20 L optimized process. Non-reducing (Lanes 1, 4) and reducing (Lanes 2, 3) conditions. Western blot analysis of (C) Pfs230C1 and (D) native parasite extracts with mouse monoclonal antibody 15A4-1B12 under reducing (R) and non-reducing (NR) conditions. Different molecular weight marker standards are utilized for panels C and D given the difference in molecular weight of target protein. Expected molecular weight of native Pfs230 is ∼363 kDa, however, since protease inhibitors are not utilized, multiple bands are visible.
    Topo Subcloning, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 89 stars, based on 74 article reviews
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    topo subcloning - by Bioz Stars, 2020-07
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    94
    Addgene inc subcloning
    SDS-PAGE and Western blot analysis of final purified Pfs230C1. (A) SDS-PAGE under non-reducing and reducing conditions followed by silver stain. (B) Western blot with anti-his antibody. (A–B) Lanes 1–2 representative Pfs230C1 from the original Super <t>Sf9</t> process [ 16 ], and Lanes 3–4 Pfs230C1 derived from 20 L optimized process. Non-reducing (Lanes 1, 4) and reducing (Lanes 2, 3) conditions. Western blot analysis of (C) Pfs230C1 and (D) native parasite extracts with mouse monoclonal antibody 15A4-1B12 under reducing (R) and non-reducing (NR) conditions. Different molecular weight marker standards are utilized for panels C and D given the difference in molecular weight of target protein. Expected molecular weight of native Pfs230 is ∼363 kDa, however, since protease inhibitors are not utilized, multiple bands are visible.
    Subcloning, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 230 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 230 article reviews
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    92
    Agilent technologies subcloning
    SDS-PAGE and Western blot analysis of final purified Pfs230C1. (A) SDS-PAGE under non-reducing and reducing conditions followed by silver stain. (B) Western blot with anti-his antibody. (A–B) Lanes 1–2 representative Pfs230C1 from the original Super <t>Sf9</t> process [ 16 ], and Lanes 3–4 Pfs230C1 derived from 20 L optimized process. Non-reducing (Lanes 1, 4) and reducing (Lanes 2, 3) conditions. Western blot analysis of (C) Pfs230C1 and (D) native parasite extracts with mouse monoclonal antibody 15A4-1B12 under reducing (R) and non-reducing (NR) conditions. Different molecular weight marker standards are utilized for panels C and D given the difference in molecular weight of target protein. Expected molecular weight of native Pfs230 is ∼363 kDa, however, since protease inhibitors are not utilized, multiple bands are visible.
    Subcloning, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/subcloning/product/Agilent technologies
    Average 92 stars, based on 60 article reviews
    Price from $9.99 to $1999.99
    subcloning - by Bioz Stars, 2020-07
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    Image Search Results


    SDS-PAGE and Western blot analysis of final purified Pfs230C1. (A) SDS-PAGE under non-reducing and reducing conditions followed by silver stain. (B) Western blot with anti-his antibody. (A–B) Lanes 1–2 representative Pfs230C1 from the original Super Sf9 process [ 16 ], and Lanes 3–4 Pfs230C1 derived from 20 L optimized process. Non-reducing (Lanes 1, 4) and reducing (Lanes 2, 3) conditions. Western blot analysis of (C) Pfs230C1 and (D) native parasite extracts with mouse monoclonal antibody 15A4-1B12 under reducing (R) and non-reducing (NR) conditions. Different molecular weight marker standards are utilized for panels C and D given the difference in molecular weight of target protein. Expected molecular weight of native Pfs230 is ∼363 kDa, however, since protease inhibitors are not utilized, multiple bands are visible.

    Journal: Protein Expression and Purification

    Article Title: Expression and purification optimization of an N-terminal Pfs230 transmission-blocking vaccine candidate

    doi: 10.1016/j.pep.2019.04.001

    Figure Lengend Snippet: SDS-PAGE and Western blot analysis of final purified Pfs230C1. (A) SDS-PAGE under non-reducing and reducing conditions followed by silver stain. (B) Western blot with anti-his antibody. (A–B) Lanes 1–2 representative Pfs230C1 from the original Super Sf9 process [ 16 ], and Lanes 3–4 Pfs230C1 derived from 20 L optimized process. Non-reducing (Lanes 1, 4) and reducing (Lanes 2, 3) conditions. Western blot analysis of (C) Pfs230C1 and (D) native parasite extracts with mouse monoclonal antibody 15A4-1B12 under reducing (R) and non-reducing (NR) conditions. Different molecular weight marker standards are utilized for panels C and D given the difference in molecular weight of target protein. Expected molecular weight of native Pfs230 is ∼363 kDa, however, since protease inhibitors are not utilized, multiple bands are visible.

    Article Snippet: Super Sf9 is a genetically engineered Sf9 subclone (Oxford Expression Technologies).

    Techniques: SDS Page, Western Blot, Purification, Silver Staining, Derivative Assay, Molecular Weight, Marker

    SDS-PAGE Freeze/thaw stability analysis of final purified Pfs230C1 under non-reducing (A) and reducing (B) conditions followed by silver stain. Reference Pfs230C1 from previous [ 16 ] Super Sf9 process (Lanes 1–2). Pfs230C1 from 20 L optimized process after one (Lanes 3–4) three (Lanes 5–6) and five (lanes 7–8) freeze/thaw cycles. Proteins loaded at 1 μg (Lanes 1, 3, 5, 7) and 5 μg (Lanes 2, 4, 6, 8).

    Journal: Protein Expression and Purification

    Article Title: Expression and purification optimization of an N-terminal Pfs230 transmission-blocking vaccine candidate

    doi: 10.1016/j.pep.2019.04.001

    Figure Lengend Snippet: SDS-PAGE Freeze/thaw stability analysis of final purified Pfs230C1 under non-reducing (A) and reducing (B) conditions followed by silver stain. Reference Pfs230C1 from previous [ 16 ] Super Sf9 process (Lanes 1–2). Pfs230C1 from 20 L optimized process after one (Lanes 3–4) three (Lanes 5–6) and five (lanes 7–8) freeze/thaw cycles. Proteins loaded at 1 μg (Lanes 1, 3, 5, 7) and 5 μg (Lanes 2, 4, 6, 8).

    Article Snippet: Super Sf9 is a genetically engineered Sf9 subclone (Oxford Expression Technologies).

    Techniques: SDS Page, Purification, Silver Staining

    (A) SDS-PAGE and (B) Western blot (anti-his antibody) analysis of purified Pfs230C1 from Super Sf9 (Lanes 1–2), Sf9 (Lanes 3–4) and High Five cells (Lanes 5–6). Samples loaded under non-reducing (Lanes 1, 3, 5) and reducing (Lanes 2, 4, 6) conditions. Arrows indicate the target Pfs230C1 protein.

    Journal: Protein Expression and Purification

    Article Title: Expression and purification optimization of an N-terminal Pfs230 transmission-blocking vaccine candidate

    doi: 10.1016/j.pep.2019.04.001

    Figure Lengend Snippet: (A) SDS-PAGE and (B) Western blot (anti-his antibody) analysis of purified Pfs230C1 from Super Sf9 (Lanes 1–2), Sf9 (Lanes 3–4) and High Five cells (Lanes 5–6). Samples loaded under non-reducing (Lanes 1, 3, 5) and reducing (Lanes 2, 4, 6) conditions. Arrows indicate the target Pfs230C1 protein.

    Article Snippet: Super Sf9 is a genetically engineered Sf9 subclone (Oxford Expression Technologies).

    Techniques: SDS Page, Western Blot, Purification