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  • 99
    New England Biolabs alexa fluor 647 conjugated streptavidin
    Alexa Fluor 647 Conjugated Streptavidin, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 647 conjugated streptavidin/product/New England Biolabs
    Average 99 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 647 conjugated streptavidin - by Bioz Stars, 2020-08
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    99
    Thermo Fisher alexa fluor 647 streptavidin
    Synergistic activation of NF-κB by NKG2D and 2B4 co-engagement. ( a ) NKL cells rested in the absence of IL-2 for 24 h were stimulated with NKG2D and/or 2B4 by receptor crosslinking for the indicated time. Cell lysates were immunoblotted with Abs to phospho-p65 at serine 536 (pS536), p65, phospho-IKKα/β at serine 176/180 (pS176/180), IKKβ, phospho-Akt at serine 473 (pS473), phospho-Erk1 and 2, or actin. The normalized intensities of the phosphorylated p65 and IKKα/β relative to their total forms are presented. ( b ) Rested NKL cells were treated as in a to stimulate NKG2D and 2B4 for the indicated time. Lysates were immunoblotted for phospho-p65, phospho-IKKα/β, phospho-IκBα at serine 32/36 (pS32/36), IκBα, phospho-Erk1/2 or actin. ( c ) Representative confocal images (top) of conjugates between rested NKL cells loaded with CFSE (green) and P815 target cells as indicated. Conjugates were fixed, permeabilized and stained with 4,6-diamidino-2-phenylindole (DAPI; blue) and mAb to p65, anti-mouse IgG-Biotin followed with <t>Alexa</t> <t>Fluor</t> 647 <t>(red)-Streptavidin.</t> The number beneath the overlay image is the mean nuclear fluorescence intensity (MFI)±s.d. of p65 from ⩾50 NKL-target cell conjugates. Statistical bar charts (bottom) for MFI of p65 in the nucleus are represented as fold change. Values represent mean±s.d. Scale bar, 5 μm. ( d ) Rested NKL cells were stimulated with plate-immobilized mAbs to NKG2D and/or 2B4 for 1 h. Equal amounts of protein from cytoplasmic and nuclear extracts were immunoblotted with mAb to p65. ( e ) Nuclear extracts collected as in d were added into a 96-well plate immobilized with double-stranded oligonucleotide containing the consensus NF-κB-binding sequence. The amount of p65 bound to the oligonucleotide was measured by colorimetric assay. Values represent mean±s.d. ( f ) Rested NKL cells transduced with a κB-GFP reporter construct were stimulated with plate-immobilized mAbs to NKG2D and/or 2B4 for 6 h. GFP expression in NKL-κB-GFP cells was analysed by flow cytometry, and representative result (top) and statistical bar charts (bottom) are shown. Values represent mean±s.d. P
    Alexa Fluor 647 Streptavidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 211 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 211 article reviews
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    95
    BioLegend streptavidin alexa fluor 647
    Synergistic activation of NF-κB by NKG2D and 2B4 co-engagement. ( a ) NKL cells rested in the absence of IL-2 for 24 h were stimulated with NKG2D and/or 2B4 by receptor crosslinking for the indicated time. Cell lysates were immunoblotted with Abs to phospho-p65 at serine 536 (pS536), p65, phospho-IKKα/β at serine 176/180 (pS176/180), IKKβ, phospho-Akt at serine 473 (pS473), phospho-Erk1 and 2, or actin. The normalized intensities of the phosphorylated p65 and IKKα/β relative to their total forms are presented. ( b ) Rested NKL cells were treated as in a to stimulate NKG2D and 2B4 for the indicated time. Lysates were immunoblotted for phospho-p65, phospho-IKKα/β, phospho-IκBα at serine 32/36 (pS32/36), IκBα, phospho-Erk1/2 or actin. ( c ) Representative confocal images (top) of conjugates between rested NKL cells loaded with CFSE (green) and P815 target cells as indicated. Conjugates were fixed, permeabilized and stained with 4,6-diamidino-2-phenylindole (DAPI; blue) and mAb to p65, anti-mouse IgG-Biotin followed with <t>Alexa</t> <t>Fluor</t> 647 <t>(red)-Streptavidin.</t> The number beneath the overlay image is the mean nuclear fluorescence intensity (MFI)±s.d. of p65 from ⩾50 NKL-target cell conjugates. Statistical bar charts (bottom) for MFI of p65 in the nucleus are represented as fold change. Values represent mean±s.d. Scale bar, 5 μm. ( d ) Rested NKL cells were stimulated with plate-immobilized mAbs to NKG2D and/or 2B4 for 1 h. Equal amounts of protein from cytoplasmic and nuclear extracts were immunoblotted with mAb to p65. ( e ) Nuclear extracts collected as in d were added into a 96-well plate immobilized with double-stranded oligonucleotide containing the consensus NF-κB-binding sequence. The amount of p65 bound to the oligonucleotide was measured by colorimetric assay. Values represent mean±s.d. ( f ) Rested NKL cells transduced with a κB-GFP reporter construct were stimulated with plate-immobilized mAbs to NKG2D and/or 2B4 for 6 h. GFP expression in NKL-κB-GFP cells was analysed by flow cytometry, and representative result (top) and statistical bar charts (bottom) are shown. Values represent mean±s.d. P
    Streptavidin Alexa Fluor 647, supplied by BioLegend, used in various techniques. Bioz Stars score: 95/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 23 article reviews
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    98
    Thermo Fisher streptavidin alexa fluor 647
    Glycosylation of the CRD of DCIR modulates its binding to glycan structures. (A) Schematic overview of the N -glycosylation site inside the putative CRD of DCIR. NES is the only predicted N -glycosylation site of DCIR. The EPS motif is the predicted glycan binding motif. Adapted from Bates et al . [(6)] . (B) Increased binding to DCIR ligands in the presence of truncated or complete absence of glycans on the DCIR-Fc construct. Binding of wild-type and the glycosylation mutant DCIR-Fc to immobilized neoglycoconjugates was measured in a glycan binding assay. Average DCIR-Fc binding ± SEM was calculated from 4 independent experiments. (C) DCIR expression on DCIR transduced cell lines was determined by flow cytometry. Filled histogram represents isotype control and black line indicates the DCIR expression. (D) Binding of glycan beads to cellular DCIR could not be detected. Binding of fluorescent labeled glycan beads to the different DCIR expressing cell lines was measured by flow cytometry in the absence of serum. As a control binding of the fluorescent labeled glycan beads to CHO-DC-SIGN was measured. One representative experiment out of 3 is shown. (E) Glycan binding of PAA-glycans to cellular DCIR could not be detected. Binding of biotinylated PAA-glycans pre-incubated with <t>streptavidin-Alexa</t> <t>Fluor</t> 647 to the different DCIR and DC-SIGN expressing cell lines was measured by flow cytometry after 2 hours incubation at 37°C. Binding of biotinylated PAA-glycans to CHO-DC-SIGN served as a positive control. One representative experiment out of 3 is shown.
    Streptavidin Alexa Fluor 647, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 764 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin alexa fluor 647/product/Thermo Fisher
    Average 98 stars, based on 764 article reviews
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    99
    Thermo Fisher streptavidin
    Glycosylation of the CRD of DCIR modulates its binding to glycan structures. (A) Schematic overview of the N -glycosylation site inside the putative CRD of DCIR. NES is the only predicted N -glycosylation site of DCIR. The EPS motif is the predicted glycan binding motif. Adapted from Bates et al . [(6)] . (B) Increased binding to DCIR ligands in the presence of truncated or complete absence of glycans on the DCIR-Fc construct. Binding of wild-type and the glycosylation mutant DCIR-Fc to immobilized neoglycoconjugates was measured in a glycan binding assay. Average DCIR-Fc binding ± SEM was calculated from 4 independent experiments. (C) DCIR expression on DCIR transduced cell lines was determined by flow cytometry. Filled histogram represents isotype control and black line indicates the DCIR expression. (D) Binding of glycan beads to cellular DCIR could not be detected. Binding of fluorescent labeled glycan beads to the different DCIR expressing cell lines was measured by flow cytometry in the absence of serum. As a control binding of the fluorescent labeled glycan beads to CHO-DC-SIGN was measured. One representative experiment out of 3 is shown. (E) Glycan binding of PAA-glycans to cellular DCIR could not be detected. Binding of biotinylated PAA-glycans pre-incubated with <t>streptavidin-Alexa</t> <t>Fluor</t> 647 to the different DCIR and DC-SIGN expressing cell lines was measured by flow cytometry after 2 hours incubation at 37°C. Binding of biotinylated PAA-glycans to CHO-DC-SIGN served as a positive control. One representative experiment out of 3 is shown.
    Streptavidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10427 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin/product/Thermo Fisher
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    streptavidin - by Bioz Stars, 2020-08
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    94
    Jackson Immuno alexa fluor 647 streptavidin
    Glycosylation of the CRD of DCIR modulates its binding to glycan structures. (A) Schematic overview of the N -glycosylation site inside the putative CRD of DCIR. NES is the only predicted N -glycosylation site of DCIR. The EPS motif is the predicted glycan binding motif. Adapted from Bates et al . [(6)] . (B) Increased binding to DCIR ligands in the presence of truncated or complete absence of glycans on the DCIR-Fc construct. Binding of wild-type and the glycosylation mutant DCIR-Fc to immobilized neoglycoconjugates was measured in a glycan binding assay. Average DCIR-Fc binding ± SEM was calculated from 4 independent experiments. (C) DCIR expression on DCIR transduced cell lines was determined by flow cytometry. Filled histogram represents isotype control and black line indicates the DCIR expression. (D) Binding of glycan beads to cellular DCIR could not be detected. Binding of fluorescent labeled glycan beads to the different DCIR expressing cell lines was measured by flow cytometry in the absence of serum. As a control binding of the fluorescent labeled glycan beads to CHO-DC-SIGN was measured. One representative experiment out of 3 is shown. (E) Glycan binding of PAA-glycans to cellular DCIR could not be detected. Binding of biotinylated PAA-glycans pre-incubated with <t>streptavidin-Alexa</t> <t>Fluor</t> 647 to the different DCIR and DC-SIGN expressing cell lines was measured by flow cytometry after 2 hours incubation at 37°C. Binding of biotinylated PAA-glycans to CHO-DC-SIGN served as a positive control. One representative experiment out of 3 is shown.
    Alexa Fluor 647 Streptavidin, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    GE Healthcare streptavidin alexa fluor 647
    Glycosylation of the CRD of DCIR modulates its binding to glycan structures. (A) Schematic overview of the N -glycosylation site inside the putative CRD of DCIR. NES is the only predicted N -glycosylation site of DCIR. The EPS motif is the predicted glycan binding motif. Adapted from Bates et al . [(6)] . (B) Increased binding to DCIR ligands in the presence of truncated or complete absence of glycans on the DCIR-Fc construct. Binding of wild-type and the glycosylation mutant DCIR-Fc to immobilized neoglycoconjugates was measured in a glycan binding assay. Average DCIR-Fc binding ± SEM was calculated from 4 independent experiments. (C) DCIR expression on DCIR transduced cell lines was determined by flow cytometry. Filled histogram represents isotype control and black line indicates the DCIR expression. (D) Binding of glycan beads to cellular DCIR could not be detected. Binding of fluorescent labeled glycan beads to the different DCIR expressing cell lines was measured by flow cytometry in the absence of serum. As a control binding of the fluorescent labeled glycan beads to CHO-DC-SIGN was measured. One representative experiment out of 3 is shown. (E) Glycan binding of PAA-glycans to cellular DCIR could not be detected. Binding of biotinylated PAA-glycans pre-incubated with <t>streptavidin-Alexa</t> <t>Fluor</t> 647 to the different DCIR and DC-SIGN expressing cell lines was measured by flow cytometry after 2 hours incubation at 37°C. Binding of biotinylated PAA-glycans to CHO-DC-SIGN served as a positive control. One representative experiment out of 3 is shown.
    Streptavidin Alexa Fluor 647, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin alexa fluor 647/product/GE Healthcare
    Average 91 stars, based on 5 article reviews
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    88
    Thermo Fisher streptavidin conjugated alexa fluor 647
    Whole-Mount S. mansoni Adult Worm Immunofluorescence S. mansoni adult worms were probed with anti-SmTβRII rabbit IgG and pre-immune rabbit IgG ( Figure S2 ), followed by biotin-conjugated anti-rabbit IgG. Reactive complexes were detected using <t>streptavidin</t> <t>Alexa</t> <t>Fluor</t> 647 conjugate and analyzed with a Bio-Rad MRC1024 confocal laser microscope. Anti-SmTβRII reactivity is shown in a live male worm (♂) (panels A–C) in different laser sections in tubercles (T) (panel B) and gynaecophoric canal (G) (panel C). Specific surface fluorescence is also shown in live female worms (♀) (panels F and I), whereas green fluorescence fields show the non-specific auto-fluorescence in vitellaria (V), oviduct (OvD) (panel E), and ova (Ov) (panels E and H). An acetone-fixed male worm (♂) shows anti-SmTβRII reactivity in the gynaecophoric canal, oral (Os) and ventral suckers (Vs) (panel K), and in esophagus (O) (panel L). Panels A, D, G, and J are phase-contrast fields of the fluorescent fields B and C, E and F, H and I, and K and L, respectively.
    Streptavidin Conjugated Alexa Fluor 647, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher streptavidin alexa647
    Targeting of cells with biotin-NLP-RPE liposome. ( a ) Gli36 human glioma cells expressing the EGFRvIII receptor and GFP were labeled with a biotinylated-antibody against EGFRvIII followed by <t>streaptavidin-Alexa647</t> followed biotin-NLP-RPE, and analyzed by fluorescence microscopy for both Alexa647 and RPE. ( b ) Gli36 cells were infected with lentivirus vector expressing BAP-TM and GFP. These cells were labeled with <t>Streptavidin-Alexa647</t> followed by biotin-NLP-RPE and analyzed as in ( a ).
    Streptavidin Alexa647, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 169 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin alexa647/product/Thermo Fisher
    Average 88 stars, based on 169 article reviews
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    Thermo Fisher alexa647 conjugated streptavidin
    Principal neurons PNs innervate the 2 basket cell (BC) types differently. Maximum z intensity projection image of a representative PN→parvalbumin-containing basket cell (PVBC) pair (A) and a PN→cholecystokinin-expressing basket cells (CCKBC) pair (F). Cascade Blue was used to label the BCs, while biocytin was introduced into the PNs to visualize them with <t>streptavidin-conjugated</t> <t>Alexa647.</t> Scales for (A and F): 50 μm. Neurolucida reconstructions (B and G) and the resulted dendrogram analysis (C and H) of the PVBC and CCKBC shown in (A) and in (F), respectively, marking the location of connections 1 and 2 (c1 and c2) established by the monosynaptically connected presynaptic PNs. Scales for (B, C, G, H): 50 μm. (D and E) High-power magnification 3D confocal images of putative contacts c1 and c2, showing close appositions between the dendrite-targeting boutons of the PN (yellow) and the PVBC dendrites (blue). Scale: 2 μm. (I) High power magnification 3D confocal images of a putative contact c1, showing close appositions between the bouton of the PN (yellow) and the CCKBC dendrites (blue). Scales: 2 μm. (J) PNs establish significantly more contacts on PVBCs than on CCKBCs (1.79 ± 0.26, n = 14 PN→CCKBC pairs, 3.23 ± 0.57, n = 13 PN→PVBC pairs, Mann–Whitney U test, * p
    Alexa647 Conjugated Streptavidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa647 conjugated streptavidin/product/Thermo Fisher
    Average 88 stars, based on 97 article reviews
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    Image Search Results


    Synergistic activation of NF-κB by NKG2D and 2B4 co-engagement. ( a ) NKL cells rested in the absence of IL-2 for 24 h were stimulated with NKG2D and/or 2B4 by receptor crosslinking for the indicated time. Cell lysates were immunoblotted with Abs to phospho-p65 at serine 536 (pS536), p65, phospho-IKKα/β at serine 176/180 (pS176/180), IKKβ, phospho-Akt at serine 473 (pS473), phospho-Erk1 and 2, or actin. The normalized intensities of the phosphorylated p65 and IKKα/β relative to their total forms are presented. ( b ) Rested NKL cells were treated as in a to stimulate NKG2D and 2B4 for the indicated time. Lysates were immunoblotted for phospho-p65, phospho-IKKα/β, phospho-IκBα at serine 32/36 (pS32/36), IκBα, phospho-Erk1/2 or actin. ( c ) Representative confocal images (top) of conjugates between rested NKL cells loaded with CFSE (green) and P815 target cells as indicated. Conjugates were fixed, permeabilized and stained with 4,6-diamidino-2-phenylindole (DAPI; blue) and mAb to p65, anti-mouse IgG-Biotin followed with Alexa Fluor 647 (red)-Streptavidin. The number beneath the overlay image is the mean nuclear fluorescence intensity (MFI)±s.d. of p65 from ⩾50 NKL-target cell conjugates. Statistical bar charts (bottom) for MFI of p65 in the nucleus are represented as fold change. Values represent mean±s.d. Scale bar, 5 μm. ( d ) Rested NKL cells were stimulated with plate-immobilized mAbs to NKG2D and/or 2B4 for 1 h. Equal amounts of protein from cytoplasmic and nuclear extracts were immunoblotted with mAb to p65. ( e ) Nuclear extracts collected as in d were added into a 96-well plate immobilized with double-stranded oligonucleotide containing the consensus NF-κB-binding sequence. The amount of p65 bound to the oligonucleotide was measured by colorimetric assay. Values represent mean±s.d. ( f ) Rested NKL cells transduced with a κB-GFP reporter construct were stimulated with plate-immobilized mAbs to NKG2D and/or 2B4 for 6 h. GFP expression in NKL-κB-GFP cells was analysed by flow cytometry, and representative result (top) and statistical bar charts (bottom) are shown. Values represent mean±s.d. P

    Journal: Nature Communications

    Article Title: Stepwise phosphorylation of p65 promotes NF-κB activation and NK cell responses during target cell recognition

    doi: 10.1038/ncomms11686

    Figure Lengend Snippet: Synergistic activation of NF-κB by NKG2D and 2B4 co-engagement. ( a ) NKL cells rested in the absence of IL-2 for 24 h were stimulated with NKG2D and/or 2B4 by receptor crosslinking for the indicated time. Cell lysates were immunoblotted with Abs to phospho-p65 at serine 536 (pS536), p65, phospho-IKKα/β at serine 176/180 (pS176/180), IKKβ, phospho-Akt at serine 473 (pS473), phospho-Erk1 and 2, or actin. The normalized intensities of the phosphorylated p65 and IKKα/β relative to their total forms are presented. ( b ) Rested NKL cells were treated as in a to stimulate NKG2D and 2B4 for the indicated time. Lysates were immunoblotted for phospho-p65, phospho-IKKα/β, phospho-IκBα at serine 32/36 (pS32/36), IκBα, phospho-Erk1/2 or actin. ( c ) Representative confocal images (top) of conjugates between rested NKL cells loaded with CFSE (green) and P815 target cells as indicated. Conjugates were fixed, permeabilized and stained with 4,6-diamidino-2-phenylindole (DAPI; blue) and mAb to p65, anti-mouse IgG-Biotin followed with Alexa Fluor 647 (red)-Streptavidin. The number beneath the overlay image is the mean nuclear fluorescence intensity (MFI)±s.d. of p65 from ⩾50 NKL-target cell conjugates. Statistical bar charts (bottom) for MFI of p65 in the nucleus are represented as fold change. Values represent mean±s.d. Scale bar, 5 μm. ( d ) Rested NKL cells were stimulated with plate-immobilized mAbs to NKG2D and/or 2B4 for 1 h. Equal amounts of protein from cytoplasmic and nuclear extracts were immunoblotted with mAb to p65. ( e ) Nuclear extracts collected as in d were added into a 96-well plate immobilized with double-stranded oligonucleotide containing the consensus NF-κB-binding sequence. The amount of p65 bound to the oligonucleotide was measured by colorimetric assay. Values represent mean±s.d. ( f ) Rested NKL cells transduced with a κB-GFP reporter construct were stimulated with plate-immobilized mAbs to NKG2D and/or 2B4 for 6 h. GFP expression in NKL-κB-GFP cells was analysed by flow cytometry, and representative result (top) and statistical bar charts (bottom) are shown. Values represent mean±s.d. P

    Article Snippet: CFSE, α-mouse IgG-Biotin and Alexa Fluor 647-Streptavidin were obtained from Invitrogen for use in confocal microscopy.

    Techniques: Activation Assay, Staining, Fluorescence, Binding Assay, Sequencing, Colorimetric Assay, Transduction, Construct, Expressing, Flow Cytometry, Cytometry

    Triple immunofluorescence staining using three mouse antibodies on mouse intestinal tissue. Anti-smooth muscle actin (SMA) labeled with Alexa Fluor 647 and pseudo-colored white (A), anti-β−catenin labeled with Alexa Fluor 568 and pseudo-colored red (B), anti-muscle actin (HHF35) labeled with FITC and pseudo-colored green (C), and the merged image showing all three antibodies (D). The tissue section was counterstained with DAPI (pseudo-colored blue). Images were captured at 10×. Scale bar is 100 µm.

    Journal: Journal of Histochemistry and Cytochemistry

    Article Title: A Flexible Mouse-On-Mouse Immunohistochemical Staining Technique Adaptable to Biotin-Free Reagents, Immunofluorescence, and Multiple Antibody Staining

    doi: 10.1369/0022155413511620

    Figure Lengend Snippet: Triple immunofluorescence staining using three mouse antibodies on mouse intestinal tissue. Anti-smooth muscle actin (SMA) labeled with Alexa Fluor 647 and pseudo-colored white (A), anti-β−catenin labeled with Alexa Fluor 568 and pseudo-colored red (B), anti-muscle actin (HHF35) labeled with FITC and pseudo-colored green (C), and the merged image showing all three antibodies (D). The tissue section was counterstained with DAPI (pseudo-colored blue). Images were captured at 10×. Scale bar is 100 µm.

    Article Snippet: This was followed with an Alexa Fluor 647-labeled Streptavidin 1:200 (# , Invitrogen; Carlsbad, CA).

    Techniques: Immunofluorescence, Staining, Labeling

    Preparation of soluble siglecs and protein–glycan interactions measured on glycan arrays. (A) Western blot of soluble material released from siglec-expressing CHO cells either treated (+) or not treated (−) with PP. Supernatants containing cleaved siglec-7–EGFP (S7), siglec-8–EGFP (S8), and siglec-9–EGFP (S9) were probed with biotinylated goat anti-GFP followed by streptavidin–alkaline phosphatase. (B) Western blot of cleaved material either pretreated (+) or not pretreated (−) with BirA enzyme and probed with streptavidin–alkaline phosphatase. (C) Dose–response microarray analysis of the binding of siglecs to lipid-linked oligosaccharide probes. Microarrays of six oligosaccharide probes (structures are shown in Table 1 ) were generated on nitrocellulose-coated glass slides. Each probe was printed in duplicate at 1 ( ), 1.33 ( ), 1.66 ( ), 2.33 ( ), 3.33 ( ), and 5 ( ) fmol/spot. Binding of biotinylated siglec-7–EGFP (top panel), siglec-8–EGFP (middle panel), and siglec-9–EGFP (bottom panel) chimeras was detected with AlexaFluor-647-labeled streptavidin as described in Materials and methods.

    Journal: Analytical Biochemistry

    Article Title: An expression system for screening of proteins for glycan and protein interactions

    doi: 10.1016/j.ab.2010.12.036

    Figure Lengend Snippet: Preparation of soluble siglecs and protein–glycan interactions measured on glycan arrays. (A) Western blot of soluble material released from siglec-expressing CHO cells either treated (+) or not treated (−) with PP. Supernatants containing cleaved siglec-7–EGFP (S7), siglec-8–EGFP (S8), and siglec-9–EGFP (S9) were probed with biotinylated goat anti-GFP followed by streptavidin–alkaline phosphatase. (B) Western blot of cleaved material either pretreated (+) or not pretreated (−) with BirA enzyme and probed with streptavidin–alkaline phosphatase. (C) Dose–response microarray analysis of the binding of siglecs to lipid-linked oligosaccharide probes. Microarrays of six oligosaccharide probes (structures are shown in Table 1 ) were generated on nitrocellulose-coated glass slides. Each probe was printed in duplicate at 1 ( ), 1.33 ( ), 1.66 ( ), 2.33 ( ), 3.33 ( ), and 5 ( ) fmol/spot. Binding of biotinylated siglec-7–EGFP (top panel), siglec-8–EGFP (middle panel), and siglec-9–EGFP (bottom panel) chimeras was detected with AlexaFluor-647-labeled streptavidin as described in Materials and methods.

    Article Snippet: The slides were rinsed with blocking buffer and overlaid for 1.5 h with the biotinylated siglec–GFP constructs that had been precomplexed for 1 h at 4 °C with AlexaFluor-647–streptavidin (Molecular Probes) (1:1, w/w) and rabbit anti-GFP (Invitrogen) (1:6, w/w).

    Techniques: Western Blot, Expressing, Microarray, Binding Assay, Generated, Labeling

    Glycosylation of the CRD of DCIR modulates its binding to glycan structures. (A) Schematic overview of the N -glycosylation site inside the putative CRD of DCIR. NES is the only predicted N -glycosylation site of DCIR. The EPS motif is the predicted glycan binding motif. Adapted from Bates et al . [(6)] . (B) Increased binding to DCIR ligands in the presence of truncated or complete absence of glycans on the DCIR-Fc construct. Binding of wild-type and the glycosylation mutant DCIR-Fc to immobilized neoglycoconjugates was measured in a glycan binding assay. Average DCIR-Fc binding ± SEM was calculated from 4 independent experiments. (C) DCIR expression on DCIR transduced cell lines was determined by flow cytometry. Filled histogram represents isotype control and black line indicates the DCIR expression. (D) Binding of glycan beads to cellular DCIR could not be detected. Binding of fluorescent labeled glycan beads to the different DCIR expressing cell lines was measured by flow cytometry in the absence of serum. As a control binding of the fluorescent labeled glycan beads to CHO-DC-SIGN was measured. One representative experiment out of 3 is shown. (E) Glycan binding of PAA-glycans to cellular DCIR could not be detected. Binding of biotinylated PAA-glycans pre-incubated with streptavidin-Alexa Fluor 647 to the different DCIR and DC-SIGN expressing cell lines was measured by flow cytometry after 2 hours incubation at 37°C. Binding of biotinylated PAA-glycans to CHO-DC-SIGN served as a positive control. One representative experiment out of 3 is shown.

    Journal: PLoS ONE

    Article Title: Ligand Binding and Signaling of Dendritic Cell Immunoreceptor (DCIR) Is Modulated by the Glycosylation of the Carbohydrate Recognition Domain

    doi: 10.1371/journal.pone.0066266

    Figure Lengend Snippet: Glycosylation of the CRD of DCIR modulates its binding to glycan structures. (A) Schematic overview of the N -glycosylation site inside the putative CRD of DCIR. NES is the only predicted N -glycosylation site of DCIR. The EPS motif is the predicted glycan binding motif. Adapted from Bates et al . [(6)] . (B) Increased binding to DCIR ligands in the presence of truncated or complete absence of glycans on the DCIR-Fc construct. Binding of wild-type and the glycosylation mutant DCIR-Fc to immobilized neoglycoconjugates was measured in a glycan binding assay. Average DCIR-Fc binding ± SEM was calculated from 4 independent experiments. (C) DCIR expression on DCIR transduced cell lines was determined by flow cytometry. Filled histogram represents isotype control and black line indicates the DCIR expression. (D) Binding of glycan beads to cellular DCIR could not be detected. Binding of fluorescent labeled glycan beads to the different DCIR expressing cell lines was measured by flow cytometry in the absence of serum. As a control binding of the fluorescent labeled glycan beads to CHO-DC-SIGN was measured. One representative experiment out of 3 is shown. (E) Glycan binding of PAA-glycans to cellular DCIR could not be detected. Binding of biotinylated PAA-glycans pre-incubated with streptavidin-Alexa Fluor 647 to the different DCIR and DC-SIGN expressing cell lines was measured by flow cytometry after 2 hours incubation at 37°C. Binding of biotinylated PAA-glycans to CHO-DC-SIGN served as a positive control. One representative experiment out of 3 is shown.

    Article Snippet: Antibodies used are α-DCIR 111F8.04 (Dendritics), α-DC-SIGN AZN-D1 , α-Langerin 10E2 , α-phosporylated tyrosine PY20 (Millipore), goat anti-mouse IgG Alexa Fluor 488 F(ab′)2 fragment (Invitrogen), goat-anti-human Fc-PO (Jackson Immunoresearch), strepatavidin-PO (Invitrogen), goat-anti-human Fc (Jackson), goat-anti-human Fc-biotin (Jackson), streptavidin-Alexa Fluor 647 and 488 (Invitrogen) and IRDye goat-α-mouse IgG 800CW (Li-Cor).

    Techniques: Binding Assay, Construct, Mutagenesis, Expressing, Flow Cytometry, Cytometry, Labeling, Incubation, Positive Control

    Tracking pIgR and VHH across the primary human lung tissue model. (a): Heatmap showing the amount of pIgR retained in the tissue model following transcytosis. (b) Heatmap showing the amount of VHH retained in the tissue model following transcytosis. Following 48 hours post-VHH treatment, tissue samples were fixed, permeabilized and stained for hpIgR and VHH. A mouse anti-hpIgR primary antibody and Alexa-Flour 488-labeled anti-mouse secondary antibody were used to stain hpIgR. A biotinylated anti-VHH primary antibody and Alexa-Flour 647-labeled streptavidin were used to stain VHH. Indirect immunofluorescence images were collected and processed using Opera Phenix confocal laser microscopy. VHH14 was used as a negative control.

    Journal: mAbs

    Article Title: Discovery and characterization of single-domain antibodies for polymeric Ig receptor-mediated mucosal delivery of biologics

    doi: 10.1080/19420862.2019.1708030

    Figure Lengend Snippet: Tracking pIgR and VHH across the primary human lung tissue model. (a): Heatmap showing the amount of pIgR retained in the tissue model following transcytosis. (b) Heatmap showing the amount of VHH retained in the tissue model following transcytosis. Following 48 hours post-VHH treatment, tissue samples were fixed, permeabilized and stained for hpIgR and VHH. A mouse anti-hpIgR primary antibody and Alexa-Flour 488-labeled anti-mouse secondary antibody were used to stain hpIgR. A biotinylated anti-VHH primary antibody and Alexa-Flour 647-labeled streptavidin were used to stain VHH. Indirect immunofluorescence images were collected and processed using Opera Phenix confocal laser microscopy. VHH14 was used as a negative control.

    Article Snippet: The secondary antibody mix contained Alexa-Flour 488-labeled anti-mouse antibody (Agilent F0479, 1:100 dilution), Alexa-Flour 647-labeled streptavidin (Invitrogen, S32357, 1:100 dilution) and Hoechst (Invitrogen H3570, 1: 1000 dilution).

    Techniques: Staining, Labeling, Immunofluorescence, Microscopy, Negative Control

    St6gal1 is specifically up-regulated at the transcriptional level during the TGF-β-induced EMT in GE11 cells. A , RT-PCR using total RNA extracted from TGF-β-treated and -untreated cells was carried out to examine the expression levels of genes involved in protein sialylation. The expression level of Gapdh was used as a loading control. Sct , sialic acid transporter; St3gal , β-galactoside α2,3-sialyltransferase; ST6Gal , β-galactoside α2,6-sialyltransferase; St6galnac , α- N -acetylgalactosaminide α2,6-sialyltransferase; Neu , neuraminidase. B , cells treated with or without TGF-β were incubated with ( heavy line ) or without ( gray shadow ) biotin-conjugated MAA (recognizing α2,3-sialylated proteins) or biotin-conjugated SNA (recognizing α2,6-sialylated proteins), followed by incubation with streptavidin Alexa Fluor 647 conjugate, and subjected to FACS analysis. C , representative glycan profiling in the cells treated with or without TGF-β to compare the major complex type of N -glycans by LC/MS. Peak area of asialo- and sialylated N -glycans were calculated based on the extracted mass chromatograms acquired in positive and negative modes, respectively. The relative peak area of major N -glycans from those cells was presented as a percentage of the total peak area of the glycans. Glycan structures were deduced from the accurate masses and product ion spectra.

    Journal: The Journal of Biological Chemistry

    Article Title: β-Galactoside α2,6-Sialyltranferase 1 Promotes Transforming Growth Factor-β-mediated Epithelial-Mesenchymal Transition *

    doi: 10.1074/jbc.M114.593392

    Figure Lengend Snippet: St6gal1 is specifically up-regulated at the transcriptional level during the TGF-β-induced EMT in GE11 cells. A , RT-PCR using total RNA extracted from TGF-β-treated and -untreated cells was carried out to examine the expression levels of genes involved in protein sialylation. The expression level of Gapdh was used as a loading control. Sct , sialic acid transporter; St3gal , β-galactoside α2,3-sialyltransferase; ST6Gal , β-galactoside α2,6-sialyltransferase; St6galnac , α- N -acetylgalactosaminide α2,6-sialyltransferase; Neu , neuraminidase. B , cells treated with or without TGF-β were incubated with ( heavy line ) or without ( gray shadow ) biotin-conjugated MAA (recognizing α2,3-sialylated proteins) or biotin-conjugated SNA (recognizing α2,6-sialylated proteins), followed by incubation with streptavidin Alexa Fluor 647 conjugate, and subjected to FACS analysis. C , representative glycan profiling in the cells treated with or without TGF-β to compare the major complex type of N -glycans by LC/MS. Peak area of asialo- and sialylated N -glycans were calculated based on the extracted mass chromatograms acquired in positive and negative modes, respectively. The relative peak area of major N -glycans from those cells was presented as a percentage of the total peak area of the glycans. Glycan structures were deduced from the accurate masses and product ion spectra.

    Article Snippet: Then cells were stained with the 10 μg/ml biotinylated Sambucus nigra lectin (SNA), which preferentially recognizes the α2,6-sialylated products or Maackia amurensis agglutinin (MAA), which preferentially recognized α2,3-sialylated products for 30 min on ice, followed by incubation with streptavidin-conjugate Alexa Fluor 647 (Invitrogen) for 30 min on ice.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Incubation, FACS, Liquid Chromatography with Mass Spectroscopy

    Silencing ST6GAL1 partially reverses the basal mesenchymal phenotype of MDA-MB-231 cells. A , the indicated cells were collected and incubated with ( heavy line ) or without ( gray shadow ) biotin-conjugated SNA, followed by incubation with streptavidin Alexa Fluor 647 conjugate and subjected to FACS analysis to check the St6gal1 knockdown efficiency. B , RT-PCR was carried out to examine the expression levels of ST6GAL1 , E-cadherin, β1 integrin, and α- SMA in the indicated cells. The expression level of GAPDH was used as a loading control. The quantitative data are presented as means ± S.D. from three independent experiments (*, p

    Journal: The Journal of Biological Chemistry

    Article Title: β-Galactoside α2,6-Sialyltranferase 1 Promotes Transforming Growth Factor-β-mediated Epithelial-Mesenchymal Transition *

    doi: 10.1074/jbc.M114.593392

    Figure Lengend Snippet: Silencing ST6GAL1 partially reverses the basal mesenchymal phenotype of MDA-MB-231 cells. A , the indicated cells were collected and incubated with ( heavy line ) or without ( gray shadow ) biotin-conjugated SNA, followed by incubation with streptavidin Alexa Fluor 647 conjugate and subjected to FACS analysis to check the St6gal1 knockdown efficiency. B , RT-PCR was carried out to examine the expression levels of ST6GAL1 , E-cadherin, β1 integrin, and α- SMA in the indicated cells. The expression level of GAPDH was used as a loading control. The quantitative data are presented as means ± S.D. from three independent experiments (*, p

    Article Snippet: Then cells were stained with the 10 μg/ml biotinylated Sambucus nigra lectin (SNA), which preferentially recognizes the α2,6-sialylated products or Maackia amurensis agglutinin (MAA), which preferentially recognized α2,3-sialylated products for 30 min on ice, followed by incubation with streptavidin-conjugate Alexa Fluor 647 (Invitrogen) for 30 min on ice.

    Techniques: Multiple Displacement Amplification, Incubation, FACS, Reverse Transcription Polymerase Chain Reaction, Expressing

    Silencing of St6gal1 prevents the EMT-associated morphological and molecular changes upon TGF-β stimulation in GE11 cells. DOX-inducible shRNA- St6gal1 GE11 cells were grown with or without DOX for 24 h and were then treated with TGF-β and incubated for another 72 h. A , the cells were collected and incubated with ( heavy line ) or without ( gray shadow ) biotin-conjugated SNA or biotin-conjugated MAA, followed by incubation with streptavidin Alexa Fluor 647 conjugate and subjected to FACS analysis to confirm the St6gal1 knockdown efficiency. B , bright field pictures were taken to show the cell morphology. Insets , a representative cell morphology and its magnified view. Scale bar , 100 μm. The quantitative data for the number of aggregated cells relative to the total cells are presented as means ± S.D. ( error bars ) from three independent experiments (*, p

    Journal: The Journal of Biological Chemistry

    Article Title: β-Galactoside α2,6-Sialyltranferase 1 Promotes Transforming Growth Factor-β-mediated Epithelial-Mesenchymal Transition *

    doi: 10.1074/jbc.M114.593392

    Figure Lengend Snippet: Silencing of St6gal1 prevents the EMT-associated morphological and molecular changes upon TGF-β stimulation in GE11 cells. DOX-inducible shRNA- St6gal1 GE11 cells were grown with or without DOX for 24 h and were then treated with TGF-β and incubated for another 72 h. A , the cells were collected and incubated with ( heavy line ) or without ( gray shadow ) biotin-conjugated SNA or biotin-conjugated MAA, followed by incubation with streptavidin Alexa Fluor 647 conjugate and subjected to FACS analysis to confirm the St6gal1 knockdown efficiency. B , bright field pictures were taken to show the cell morphology. Insets , a representative cell morphology and its magnified view. Scale bar , 100 μm. The quantitative data for the number of aggregated cells relative to the total cells are presented as means ± S.D. ( error bars ) from three independent experiments (*, p

    Article Snippet: Then cells were stained with the 10 μg/ml biotinylated Sambucus nigra lectin (SNA), which preferentially recognizes the α2,6-sialylated products or Maackia amurensis agglutinin (MAA), which preferentially recognized α2,3-sialylated products for 30 min on ice, followed by incubation with streptavidin-conjugate Alexa Fluor 647 (Invitrogen) for 30 min on ice.

    Techniques: shRNA, Incubation, FACS

    Whole-Mount S. mansoni Adult Worm Immunofluorescence S. mansoni adult worms were probed with anti-SmTβRII rabbit IgG and pre-immune rabbit IgG ( Figure S2 ), followed by biotin-conjugated anti-rabbit IgG. Reactive complexes were detected using streptavidin Alexa Fluor 647 conjugate and analyzed with a Bio-Rad MRC1024 confocal laser microscope. Anti-SmTβRII reactivity is shown in a live male worm (♂) (panels A–C) in different laser sections in tubercles (T) (panel B) and gynaecophoric canal (G) (panel C). Specific surface fluorescence is also shown in live female worms (♀) (panels F and I), whereas green fluorescence fields show the non-specific auto-fluorescence in vitellaria (V), oviduct (OvD) (panel E), and ova (Ov) (panels E and H). An acetone-fixed male worm (♂) shows anti-SmTβRII reactivity in the gynaecophoric canal, oral (Os) and ventral suckers (Vs) (panel K), and in esophagus (O) (panel L). Panels A, D, G, and J are phase-contrast fields of the fluorescent fields B and C, E and F, H and I, and K and L, respectively.

    Journal: PLoS Pathogens

    Article Title: Schistosoma mansoni TGF-? Receptor II: Role in Host Ligand-Induced Regulation of a Schistosome Target Gene

    doi: 10.1371/journal.ppat.0020054

    Figure Lengend Snippet: Whole-Mount S. mansoni Adult Worm Immunofluorescence S. mansoni adult worms were probed with anti-SmTβRII rabbit IgG and pre-immune rabbit IgG ( Figure S2 ), followed by biotin-conjugated anti-rabbit IgG. Reactive complexes were detected using streptavidin Alexa Fluor 647 conjugate and analyzed with a Bio-Rad MRC1024 confocal laser microscope. Anti-SmTβRII reactivity is shown in a live male worm (♂) (panels A–C) in different laser sections in tubercles (T) (panel B) and gynaecophoric canal (G) (panel C). Specific surface fluorescence is also shown in live female worms (♀) (panels F and I), whereas green fluorescence fields show the non-specific auto-fluorescence in vitellaria (V), oviduct (OvD) (panel E), and ova (Ov) (panels E and H). An acetone-fixed male worm (♂) shows anti-SmTβRII reactivity in the gynaecophoric canal, oral (Os) and ventral suckers (Vs) (panel K), and in esophagus (O) (panel L). Panels A, D, G, and J are phase-contrast fields of the fluorescent fields B and C, E and F, H and I, and K and L, respectively.

    Article Snippet: DIG-labeled probe detection was achieved by using an anti-DIG monoclonal antibody (0.25 μg /ml; Roche Applied Science), followed by a biotin-conjugated goat anti-mouse antibody (Molecular Probes, Invitrogen), and the antibody complexes were visualized using streptavidin-conjugated Alexa Fluor 647 (Molecular Probes, Invitrogen) in concentrations similar to that used in the immunofluorescence assays.

    Techniques: Immunofluorescence, Microscopy, Fluorescence

    Lectin histochemistry in murine CvP. (A–J) Transverse sections of murine CvP were stained for biotinylated lectins and visualized using Alexa Fluor 488-conjugated streptavidin. (K-T) Lectin staining was performed after single-colored ISH procedures using a Plcb2 cRNA probe. Biotinylated lectins were also used. (A and K) UEA-1, (B and L) DSL, (C and M) LEL, (D and N) STL, (E and O) WGA, (F and P) RCA-1, (G and Q) Jacalin, (H and R) PHA-L, (I and S) LTL, and (J and T) AAL. Staining signals for lectins are shown in green and those for Plcb2 mRNA in magenta. Scale, 20 μm.

    Journal: Journal of Histochemistry and Cytochemistry

    Article Title: Ulex Europaeus Agglutinin-1 Is a Reliable Taste Bud Marker for In Situ Hybridization Analyses

    doi: 10.1369/0022155415626987

    Figure Lengend Snippet: Lectin histochemistry in murine CvP. (A–J) Transverse sections of murine CvP were stained for biotinylated lectins and visualized using Alexa Fluor 488-conjugated streptavidin. (K-T) Lectin staining was performed after single-colored ISH procedures using a Plcb2 cRNA probe. Biotinylated lectins were also used. (A and K) UEA-1, (B and L) DSL, (C and M) LEL, (D and N) STL, (E and O) WGA, (F and P) RCA-1, (G and Q) Jacalin, (H and R) PHA-L, (I and S) LTL, and (J and T) AAL. Staining signals for lectins are shown in green and those for Plcb2 mRNA in magenta. Scale, 20 μm.

    Article Snippet: Taste buds were stained with biotinylated-UEA-1 and Alexa Fluor 647-conjugated streptavidin (Life Technologies).

    Techniques: Staining, In Situ Hybridization, Whole Genome Amplification

    Targeting of cells with biotin-NLP-RPE liposome. ( a ) Gli36 human glioma cells expressing the EGFRvIII receptor and GFP were labeled with a biotinylated-antibody against EGFRvIII followed by streaptavidin-Alexa647 followed biotin-NLP-RPE, and analyzed by fluorescence microscopy for both Alexa647 and RPE. ( b ) Gli36 cells were infected with lentivirus vector expressing BAP-TM and GFP. These cells were labeled with Streptavidin-Alexa647 followed by biotin-NLP-RPE and analyzed as in ( a ).

    Journal: Scientific Reports

    Article Title: Multimodal targeted high relaxivity thermosensitive liposome for in vivo imaging

    doi: 10.1038/srep17220

    Figure Lengend Snippet: Targeting of cells with biotin-NLP-RPE liposome. ( a ) Gli36 human glioma cells expressing the EGFRvIII receptor and GFP were labeled with a biotinylated-antibody against EGFRvIII followed by streaptavidin-Alexa647 followed biotin-NLP-RPE, and analyzed by fluorescence microscopy for both Alexa647 and RPE. ( b ) Gli36 cells were infected with lentivirus vector expressing BAP-TM and GFP. These cells were labeled with Streptavidin-Alexa647 followed by biotin-NLP-RPE and analyzed as in ( a ).

    Article Snippet: Cells were then washed and incubated with streptavidin-Alexa647 (1:200; Molecular Probes) for 5 min.

    Techniques: Expressing, Labeling, Fluorescence, Microscopy, Infection, Plasmid Preparation

    Principal neurons PNs innervate the 2 basket cell (BC) types differently. Maximum z intensity projection image of a representative PN→parvalbumin-containing basket cell (PVBC) pair (A) and a PN→cholecystokinin-expressing basket cells (CCKBC) pair (F). Cascade Blue was used to label the BCs, while biocytin was introduced into the PNs to visualize them with streptavidin-conjugated Alexa647. Scales for (A and F): 50 μm. Neurolucida reconstructions (B and G) and the resulted dendrogram analysis (C and H) of the PVBC and CCKBC shown in (A) and in (F), respectively, marking the location of connections 1 and 2 (c1 and c2) established by the monosynaptically connected presynaptic PNs. Scales for (B, C, G, H): 50 μm. (D and E) High-power magnification 3D confocal images of putative contacts c1 and c2, showing close appositions between the dendrite-targeting boutons of the PN (yellow) and the PVBC dendrites (blue). Scale: 2 μm. (I) High power magnification 3D confocal images of a putative contact c1, showing close appositions between the bouton of the PN (yellow) and the CCKBC dendrites (blue). Scales: 2 μm. (J) PNs establish significantly more contacts on PVBCs than on CCKBCs (1.79 ± 0.26, n = 14 PN→CCKBC pairs, 3.23 ± 0.57, n = 13 PN→PVBC pairs, Mann–Whitney U test, * p

    Journal: PLoS Biology

    Article Title: Differential excitatory control of 2 parallel basket cell networks in amygdala microcircuits

    doi: 10.1371/journal.pbio.2001421

    Figure Lengend Snippet: Principal neurons PNs innervate the 2 basket cell (BC) types differently. Maximum z intensity projection image of a representative PN→parvalbumin-containing basket cell (PVBC) pair (A) and a PN→cholecystokinin-expressing basket cells (CCKBC) pair (F). Cascade Blue was used to label the BCs, while biocytin was introduced into the PNs to visualize them with streptavidin-conjugated Alexa647. Scales for (A and F): 50 μm. Neurolucida reconstructions (B and G) and the resulted dendrogram analysis (C and H) of the PVBC and CCKBC shown in (A) and in (F), respectively, marking the location of connections 1 and 2 (c1 and c2) established by the monosynaptically connected presynaptic PNs. Scales for (B, C, G, H): 50 μm. (D and E) High-power magnification 3D confocal images of putative contacts c1 and c2, showing close appositions between the dendrite-targeting boutons of the PN (yellow) and the PVBC dendrites (blue). Scale: 2 μm. (I) High power magnification 3D confocal images of a putative contact c1, showing close appositions between the bouton of the PN (yellow) and the CCKBC dendrites (blue). Scales: 2 μm. (J) PNs establish significantly more contacts on PVBCs than on CCKBCs (1.79 ± 0.26, n = 14 PN→CCKBC pairs, 3.23 ± 0.57, n = 13 PN→PVBC pairs, Mann–Whitney U test, * p

    Article Snippet: Biocytin-filled recorded cells were visualized either with Cy3, Alexa488 or Alexa647-conjugated streptavidin (1:3000, Molecular Probes or Life Technologies).

    Techniques: Expressing, MANN-WHITNEY