steponeplus thermocycler Thermo Fisher Search Results


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  • 99
    Thermo Fisher steponeplus thermocycler
    Amplification plot of SARS-CoV-2 N gene ssRNA control in (A) water, (B) spiked in sputum, (C) spiked in nasal exudate conducted in the same <t>thermocycler</t> run. Numbers indicated the log 10 copy number of the template present. No amplification was observed in the NTC. (D) Comparison of DIRECT-PCR assay amplification of SARS-CoV-2 N gene ssRNA control (blue line), N gene spiked in sputum (red line) and N gene spiked in nasal exudate (black line). Templates were ten-fold diluted in 8 orders of magnitude. 2 μL of template was used in 20 μL of PCR mastermix on the <t>benchtop</t> thermocycler. The amplification efficiency (E) was determined by plotting of mean C q values against log 10 copy number calculated using theoretical molarity of templates.
    Steponeplus Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 39123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher power sybr green pcr master mix
    Amplification plot of SARS-CoV-2 N gene ssRNA control in (A) water, (B) spiked in sputum, (C) spiked in nasal exudate conducted in the same <t>thermocycler</t> run. Numbers indicated the log 10 copy number of the template present. No amplification was observed in the NTC. (D) Comparison of DIRECT-PCR assay amplification of SARS-CoV-2 N gene ssRNA control (blue line), N gene spiked in sputum (red line) and N gene spiked in nasal exudate (black line). Templates were ten-fold diluted in 8 orders of magnitude. 2 μL of template was used in 20 μL of PCR mastermix on the <t>benchtop</t> thermocycler. The amplification efficiency (E) was determined by plotting of mean C q values against log 10 copy number calculated using theoretical molarity of templates.
    Power Sybr Green Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 69800 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher high capacity complementary dna reverse transcription kit
    Amplification plot of SARS-CoV-2 N gene ssRNA control in (A) water, (B) spiked in sputum, (C) spiked in nasal exudate conducted in the same <t>thermocycler</t> run. Numbers indicated the log 10 copy number of the template present. No amplification was observed in the NTC. (D) Comparison of DIRECT-PCR assay amplification of SARS-CoV-2 N gene ssRNA control (blue line), N gene spiked in sputum (red line) and N gene spiked in nasal exudate (black line). Templates were ten-fold diluted in 8 orders of magnitude. 2 μL of template was used in 20 μL of PCR mastermix on the <t>benchtop</t> thermocycler. The amplification efficiency (E) was determined by plotting of mean C q values against log 10 copy number calculated using theoretical molarity of templates.
    High Capacity Complementary Dna Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 377 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher rna
    Amplification plot of SARS-CoV-2 N gene ssRNA control in (A) water, (B) spiked in sputum, (C) spiked in nasal exudate conducted in the same <t>thermocycler</t> run. Numbers indicated the log 10 copy number of the template present. No amplification was observed in the NTC. (D) Comparison of DIRECT-PCR assay amplification of SARS-CoV-2 N gene ssRNA control (blue line), N gene spiked in sputum (red line) and N gene spiked in nasal exudate (black line). Templates were ten-fold diluted in 8 orders of magnitude. 2 μL of template was used in 20 μL of PCR mastermix on the <t>benchtop</t> thermocycler. The amplification efficiency (E) was determined by plotting of mean C q values against log 10 copy number calculated using theoretical molarity of templates.
    Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 206212 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher pcr master mix
    Amplification plot of SARS-CoV-2 N gene ssRNA control in (A) water, (B) spiked in sputum, (C) spiked in nasal exudate conducted in the same <t>thermocycler</t> run. Numbers indicated the log 10 copy number of the template present. No amplification was observed in the NTC. (D) Comparison of DIRECT-PCR assay amplification of SARS-CoV-2 N gene ssRNA control (blue line), N gene spiked in sputum (red line) and N gene spiked in nasal exudate (black line). Templates were ten-fold diluted in 8 orders of magnitude. 2 μL of template was used in 20 μL of PCR mastermix on the <t>benchtop</t> thermocycler. The amplification efficiency (E) was determined by plotting of mean C q values against log 10 copy number calculated using theoretical molarity of templates.
    Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8721 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher primer express software
    Amplification plot of SARS-CoV-2 N gene ssRNA control in (A) water, (B) spiked in sputum, (C) spiked in nasal exudate conducted in the same <t>thermocycler</t> run. Numbers indicated the log 10 copy number of the template present. No amplification was observed in the NTC. (D) Comparison of DIRECT-PCR assay amplification of SARS-CoV-2 N gene ssRNA control (blue line), N gene spiked in sputum (red line) and N gene spiked in nasal exudate (black line). Templates were ten-fold diluted in 8 orders of magnitude. 2 μL of template was used in 20 μL of PCR mastermix on the <t>benchtop</t> thermocycler. The amplification efficiency (E) was determined by plotting of mean C q values against log 10 copy number calculated using theoretical molarity of templates.
    Primer Express Software, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 26204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher fast sybr green master mix
    Amplification plot of SARS-CoV-2 N gene ssRNA control in (A) water, (B) spiked in sputum, (C) spiked in nasal exudate conducted in the same <t>thermocycler</t> run. Numbers indicated the log 10 copy number of the template present. No amplification was observed in the NTC. (D) Comparison of DIRECT-PCR assay amplification of SARS-CoV-2 N gene ssRNA control (blue line), N gene spiked in sputum (red line) and N gene spiked in nasal exudate (black line). Templates were ten-fold diluted in 8 orders of magnitude. 2 μL of template was used in 20 μL of PCR mastermix on the <t>benchtop</t> thermocycler. The amplification efficiency (E) was determined by plotting of mean C q values against log 10 copy number calculated using theoretical molarity of templates.
    Fast Sybr Green Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 23476 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman gene expression assays
    Amplification plot of SARS-CoV-2 N gene ssRNA control in (A) water, (B) spiked in sputum, (C) spiked in nasal exudate conducted in the same <t>thermocycler</t> run. Numbers indicated the log 10 copy number of the template present. No amplification was observed in the NTC. (D) Comparison of DIRECT-PCR assay amplification of SARS-CoV-2 N gene ssRNA control (blue line), N gene spiked in sputum (red line) and N gene spiked in nasal exudate (black line). Templates were ten-fold diluted in 8 orders of magnitude. 2 μL of template was used in 20 μL of PCR mastermix on the <t>benchtop</t> thermocycler. The amplification efficiency (E) was determined by plotting of mean C q values against log 10 copy number calculated using theoretical molarity of templates.
    Taqman Gene Expression Assays, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 48363 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher total rna
    Amplification plot of SARS-CoV-2 N gene ssRNA control in (A) water, (B) spiked in sputum, (C) spiked in nasal exudate conducted in the same <t>thermocycler</t> run. Numbers indicated the log 10 copy number of the template present. No amplification was observed in the NTC. (D) Comparison of DIRECT-PCR assay amplification of SARS-CoV-2 N gene ssRNA control (blue line), N gene spiked in sputum (red line) and N gene spiked in nasal exudate (black line). Templates were ten-fold diluted in 8 orders of magnitude. 2 μL of template was used in 20 μL of PCR mastermix on the <t>benchtop</t> thermocycler. The amplification efficiency (E) was determined by plotting of mean C q values against log 10 copy number calculated using theoretical molarity of templates.
    Total Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 471882 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher abi steponeplus thermocycler
    Amplification plot of SARS-CoV-2 N gene ssRNA control in (A) water, (B) spiked in sputum, (C) spiked in nasal exudate conducted in the same <t>thermocycler</t> run. Numbers indicated the log 10 copy number of the template present. No amplification was observed in the NTC. (D) Comparison of DIRECT-PCR assay amplification of SARS-CoV-2 N gene ssRNA control (blue line), N gene spiked in sputum (red line) and N gene spiked in nasal exudate (black line). Templates were ten-fold diluted in 8 orders of magnitude. 2 μL of template was used in 20 μL of PCR mastermix on the <t>benchtop</t> thermocycler. The amplification efficiency (E) was determined by plotting of mean C q values against log 10 copy number calculated using theoretical molarity of templates.
    Abi Steponeplus Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher sybr green pcr master mix
    Amplification plot of SARS-CoV-2 N gene ssRNA control in (A) water, (B) spiked in sputum, (C) spiked in nasal exudate conducted in the same <t>thermocycler</t> run. Numbers indicated the log 10 copy number of the template present. No amplification was observed in the NTC. (D) Comparison of DIRECT-PCR assay amplification of SARS-CoV-2 N gene ssRNA control (blue line), N gene spiked in sputum (red line) and N gene spiked in nasal exudate (black line). Templates were ten-fold diluted in 8 orders of magnitude. 2 μL of template was used in 20 μL of PCR mastermix on the <t>benchtop</t> thermocycler. The amplification efficiency (E) was determined by plotting of mean C q values against log 10 copy number calculated using theoretical molarity of templates.
    Sybr Green Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 101787 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher abi steponeplus device
    Amplification plot of SARS-CoV-2 N gene ssRNA control in (A) water, (B) spiked in sputum, (C) spiked in nasal exudate conducted in the same <t>thermocycler</t> run. Numbers indicated the log 10 copy number of the template present. No amplification was observed in the NTC. (D) Comparison of DIRECT-PCR assay amplification of SARS-CoV-2 N gene ssRNA control (blue line), N gene spiked in sputum (red line) and N gene spiked in nasal exudate (black line). Templates were ten-fold diluted in 8 orders of magnitude. 2 μL of template was used in 20 μL of PCR mastermix on the <t>benchtop</t> thermocycler. The amplification efficiency (E) was determined by plotting of mean C q values against log 10 copy number calculated using theoretical molarity of templates.
    Abi Steponeplus Device, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher gene exp cxcl8 hs00174103 m1
    Amplification plot of SARS-CoV-2 N gene ssRNA control in (A) water, (B) spiked in sputum, (C) spiked in nasal exudate conducted in the same <t>thermocycler</t> run. Numbers indicated the log 10 copy number of the template present. No amplification was observed in the NTC. (D) Comparison of DIRECT-PCR assay amplification of SARS-CoV-2 N gene ssRNA control (blue line), N gene spiked in sputum (red line) and N gene spiked in nasal exudate (black line). Templates were ten-fold diluted in 8 orders of magnitude. 2 μL of template was used in 20 μL of PCR mastermix on the <t>benchtop</t> thermocycler. The amplification efficiency (E) was determined by plotting of mean C q values against log 10 copy number calculated using theoretical molarity of templates.
    Gene Exp Cxcl8 Hs00174103 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1575 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher gene exp ifng mm00801778 m1
    Amplification plot of SARS-CoV-2 N gene ssRNA control in (A) water, (B) spiked in sputum, (C) spiked in nasal exudate conducted in the same <t>thermocycler</t> run. Numbers indicated the log 10 copy number of the template present. No amplification was observed in the NTC. (D) Comparison of DIRECT-PCR assay amplification of SARS-CoV-2 N gene ssRNA control (blue line), N gene spiked in sputum (red line) and N gene spiked in nasal exudate (black line). Templates were ten-fold diluted in 8 orders of magnitude. 2 μL of template was used in 20 μL of PCR mastermix on the <t>benchtop</t> thermocycler. The amplification efficiency (E) was determined by plotting of mean C q values against log 10 copy number calculated using theoretical molarity of templates.
    Gene Exp Ifng Mm00801778 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 640 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher mgb probes
    Amplification plot of SARS-CoV-2 N gene ssRNA control in (A) water, (B) spiked in sputum, (C) spiked in nasal exudate conducted in the same <t>thermocycler</t> run. Numbers indicated the log 10 copy number of the template present. No amplification was observed in the NTC. (D) Comparison of DIRECT-PCR assay amplification of SARS-CoV-2 N gene ssRNA control (blue line), N gene spiked in sputum (red line) and N gene spiked in nasal exudate (black line). Templates were ten-fold diluted in 8 orders of magnitude. 2 μL of template was used in 20 μL of PCR mastermix on the <t>benchtop</t> thermocycler. The amplification efficiency (E) was determined by plotting of mean C q values against log 10 copy number calculated using theoretical molarity of templates.
    Mgb Probes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 789 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Amplification plot of SARS-CoV-2 N gene ssRNA control in (A) water, (B) spiked in sputum, (C) spiked in nasal exudate conducted in the same thermocycler run. Numbers indicated the log 10 copy number of the template present. No amplification was observed in the NTC. (D) Comparison of DIRECT-PCR assay amplification of SARS-CoV-2 N gene ssRNA control (blue line), N gene spiked in sputum (red line) and N gene spiked in nasal exudate (black line). Templates were ten-fold diluted in 8 orders of magnitude. 2 μL of template was used in 20 μL of PCR mastermix on the benchtop thermocycler. The amplification efficiency (E) was determined by plotting of mean C q values against log 10 copy number calculated using theoretical molarity of templates.

    Journal: bioRxiv

    Article Title: Rapid direct nucleic acid amplification test without RNA extraction for SARS-CoV-2 using a portable PCR thermocycler

    doi: 10.1101/2020.04.17.042366

    Figure Lengend Snippet: Amplification plot of SARS-CoV-2 N gene ssRNA control in (A) water, (B) spiked in sputum, (C) spiked in nasal exudate conducted in the same thermocycler run. Numbers indicated the log 10 copy number of the template present. No amplification was observed in the NTC. (D) Comparison of DIRECT-PCR assay amplification of SARS-CoV-2 N gene ssRNA control (blue line), N gene spiked in sputum (red line) and N gene spiked in nasal exudate (black line). Templates were ten-fold diluted in 8 orders of magnitude. 2 μL of template was used in 20 μL of PCR mastermix on the benchtop thermocycler. The amplification efficiency (E) was determined by plotting of mean C q values against log 10 copy number calculated using theoretical molarity of templates.

    Article Snippet: The amplification was performed on a standard benchtop real-time thermocycler (StepOnePlus Real-Time PCR System, Applied Biosystems, USA).

    Techniques: Amplification, Polymerase Chain Reaction

    Fast DIRECT-PCR assay amplification efficiency of SARS-CoV-2 N gene plasmid spiked in sputum (red line) and N gene plasmid spiked in nasal exudate (black line) using the benchtop thermocycler (solid line) and the portable thermocycler (dotted line). Templates were ten-fold diluted in 6 orders of magnitude. 1 μL of template was used in 10 μL of PCR mastermix.

    Journal: bioRxiv

    Article Title: Rapid direct nucleic acid amplification test without RNA extraction for SARS-CoV-2 using a portable PCR thermocycler

    doi: 10.1101/2020.04.17.042366

    Figure Lengend Snippet: Fast DIRECT-PCR assay amplification efficiency of SARS-CoV-2 N gene plasmid spiked in sputum (red line) and N gene plasmid spiked in nasal exudate (black line) using the benchtop thermocycler (solid line) and the portable thermocycler (dotted line). Templates were ten-fold diluted in 6 orders of magnitude. 1 μL of template was used in 10 μL of PCR mastermix.

    Article Snippet: The amplification was performed on a standard benchtop real-time thermocycler (StepOnePlus Real-Time PCR System, Applied Biosystems, USA).

    Techniques: Polymerase Chain Reaction, Amplification, Plasmid Preparation