Journal: FEBS Open Bio
Article Title: Contribution of histone N-terminal tails to the structure and stability of nucleosomes
Figure Lengend Snippet: Effect of the removal of the histone N-terminal tails on the thermal stabilities of the nucleosomes. Each nucleosome, containing tlH2A, tlH2B, tlH3, or tlH4 (final concentration, 2.25 μM), was mixed with SYPRO Orange, and the sample temperature was increased by the StepOnePlus™ Real-Time PCR unit (Applied Biosystems). The temperature gradient was from 25 to 95 °C, in steps of 1 °C/min. Fluorescence signals of SYPRO Orange bound to the denatured histones were measured, and the thermal denaturing curves of the wt, tlH2A, tlH2B, tlH3, and tlH4 nucleosomes are shown in black, yellow, pink, blue, and green, respectively. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Article Snippet: The sample temperature was increased by the StepOnePlus™ Real-Time PCR unit (Applied Biosystems), and the fluorescence signals were measured with this system.
Techniques: Concentration Assay, Real-time Polymerase Chain Reaction, Fluorescence