steponeplus instrument Thermo Fisher Search Results


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  • 90
    Thermo Fisher steponeplus real time pcr system
    Analysis of mRNA and protein levels for pRb and E2F1 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of pRb and E2F1 were quantified by <t>qRT-PCR</t> on a <t>StepOnePlus</t> real-time PCR system. B. The protein levels of pRb and E2F1 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P
    Steponeplus Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 39878 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 39878 article reviews
    Price from $9.99 to $1999.99
    steponeplus real time pcr system - by Bioz Stars, 2020-02
    90/100 stars
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    99
    Thermo Fisher steponeplus instrument
    Analysis of mRNA and protein levels for pRb and E2F1 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of pRb and E2F1 were quantified by <t>qRT-PCR</t> on a <t>StepOnePlus</t> real-time PCR system. B. The protein levels of pRb and E2F1 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P
    Steponeplus Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1820 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 1820 article reviews
    Price from $9.99 to $1999.99
    steponeplus instrument - by Bioz Stars, 2020-02
    99/100 stars
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    99
    Thermo Fisher abi steponeplus instrument
    Analysis of mRNA and protein levels for pRb and E2F1 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of pRb and E2F1 were quantified by <t>qRT-PCR</t> on a <t>StepOnePlus</t> real-time PCR system. B. The protein levels of pRb and E2F1 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P
    Abi Steponeplus Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 183 article reviews
    Price from $9.99 to $1999.99
    abi steponeplus instrument - by Bioz Stars, 2020-02
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    79
    Thermo Fisher abi steponeplus system instrument
    Analysis of mRNA and protein levels for pRb and E2F1 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of pRb and E2F1 were quantified by <t>qRT-PCR</t> on a <t>StepOnePlus</t> real-time PCR system. B. The protein levels of pRb and E2F1 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P
    Abi Steponeplus System Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 79 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
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    87
    Thermo Fisher steponeplus instrument cycler
    Analysis of mRNA and protein levels for pRb and E2F1 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of pRb and E2F1 were quantified by <t>qRT-PCR</t> on a <t>StepOnePlus</t> real-time PCR system. B. The protein levels of pRb and E2F1 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P
    Steponeplus Instrument Cycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 87 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
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    93
    Thermo Fisher steponeplus real time instrument
    Analysis of mRNA and protein levels for pRb and E2F1 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of pRb and E2F1 were quantified by <t>qRT-PCR</t> on a <t>StepOnePlus</t> real-time PCR system. B. The protein levels of pRb and E2F1 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P
    Steponeplus Real Time Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 50 article reviews
    Price from $9.99 to $1999.99
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    99
    Thermo Fisher steponeplus qpcr instrument
    Analysis of mRNA and protein levels for pRb and E2F1 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of pRb and E2F1 were quantified by <t>qRT-PCR</t> on a <t>StepOnePlus</t> real-time PCR system. B. The protein levels of pRb and E2F1 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P
    Steponeplus Qpcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 52 article reviews
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    78
    Thermo Fisher steponeplus rtpcr instrument
    Analysis of mRNA and protein levels for pRb and E2F1 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of pRb and E2F1 were quantified by <t>qRT-PCR</t> on a <t>StepOnePlus</t> real-time PCR system. B. The protein levels of pRb and E2F1 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P
    Steponeplus Rtpcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 78 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
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    99
    Thermo Fisher quantitative pcr instrument
    Analysis of mRNA and protein levels for pRb and E2F1 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of pRb and E2F1 were quantified by <t>qRT-PCR</t> on a <t>StepOnePlus</t> real-time PCR system. B. The protein levels of pRb and E2F1 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P
    Quantitative Pcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 100 article reviews
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    87
    Thermo Fisher steponeplus instrument system
    Analysis of mRNA and protein levels for pRb and E2F1 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of pRb and E2F1 were quantified by <t>qRT-PCR</t> on a <t>StepOnePlus</t> real-time PCR system. B. The protein levels of pRb and E2F1 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P
    Steponeplus Instrument System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 87 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
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    78
    Thermo Fisher abi prism 7700 steponeplus instrument
    Analysis of mRNA and protein levels for pRb and E2F1 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of pRb and E2F1 were quantified by <t>qRT-PCR</t> on a <t>StepOnePlus</t> real-time PCR system. B. The protein levels of pRb and E2F1 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P
    Abi Prism 7700 Steponeplus Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 78 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
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    99
    Thermo Fisher steponeplus device
    Analysis of mRNA and protein levels for pRb and E2F1 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of pRb and E2F1 were quantified by <t>qRT-PCR</t> on a <t>StepOnePlus</t> real-time PCR system. B. The protein levels of pRb and E2F1 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P
    Steponeplus Device, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 183 article reviews
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    78
    Thermo Fisher 7500 steponeplus instrument
    Analysis of mRNA and protein levels for pRb and E2F1 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of pRb and E2F1 were quantified by <t>qRT-PCR</t> on a <t>StepOnePlus</t> real-time PCR system. B. The protein levels of pRb and E2F1 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P
    7500 Steponeplus Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 78 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    7500 steponeplus instrument - by Bioz Stars, 2020-02
    78/100 stars
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    80
    Thermo Fisher applied biosystems steponplus instrument
    Analysis of mRNA and protein levels for pRb and E2F1 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of pRb and E2F1 were quantified by <t>qRT-PCR</t> on a <t>StepOnePlus</t> real-time PCR system. B. The protein levels of pRb and E2F1 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P
    Applied Biosystems Steponplus Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 80 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    applied biosystems steponplus instrument - by Bioz Stars, 2020-02
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    77
    Thermo Fisher abi prism 7500 steponeplus instrument
    Analysis of mRNA and protein levels for pRb and E2F1 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of pRb and E2F1 were quantified by <t>qRT-PCR</t> on a <t>StepOnePlus</t> real-time PCR system. B. The protein levels of pRb and E2F1 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P
    Abi Prism 7500 Steponeplus Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 77 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    abi prism 7500 steponeplus instrument - by Bioz Stars, 2020-02
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    90
    Thermo Fisher real time pcr machine
    Analysis of mRNA and protein levels for pRb and E2F1 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of pRb and E2F1 were quantified by <t>qRT-PCR</t> on a <t>StepOnePlus</t> real-time PCR system. B. The protein levels of pRb and E2F1 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P
    Real Time Pcr Machine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1894 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1894 article reviews
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    89
    Thermo Fisher reverse transcriptase pcr rt pcr
    Analysis of mRNA and protein levels for pRb and E2F1 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of pRb and E2F1 were quantified by <t>qRT-PCR</t> on a <t>StepOnePlus</t> real-time PCR system. B. The protein levels of pRb and E2F1 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P
    Reverse Transcriptase Pcr Rt Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 673 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 89 stars, based on 673 article reviews
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    99
    Thermo Fisher steponeplus real time pcr instrument
    Analysis of mRNA and protein levels for pRb and E2F1 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of pRb and E2F1 were quantified by <t>qRT-PCR</t> on a <t>StepOnePlus</t> real-time PCR system. B. The protein levels of pRb and E2F1 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P
    Steponeplus Real Time Pcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 662 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 662 article reviews
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    92
    Thermo Fisher steponeplus qrt pcr instrument
    RNAi quantification using <t>qRT-PCR</t> and immunoblotting. A , qRT-PCR quantification of nSMase1 KD showing successful KD in nSMase1B and nSMase1C. B , qRT-PCR quantification of nSMase2 KD showing successful KD with all lentiviruses. Due to the higher level
    Steponeplus Qrt Pcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Thermo Fisher rt pcr devices
    RNAi quantification using <t>qRT-PCR</t> and immunoblotting. A , qRT-PCR quantification of nSMase1 KD showing successful KD in nSMase1B and nSMase1C. B , qRT-PCR quantification of nSMase2 KD showing successful KD with all lentiviruses. Due to the higher level
    Rt Pcr Devices, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher steponeplus real time qpcr system
    RNAi quantification using <t>qRT-PCR</t> and immunoblotting. A , qRT-PCR quantification of nSMase1 KD showing successful KD in nSMase1B and nSMase1C. B , qRT-PCR quantification of nSMase2 KD showing successful KD with all lentiviruses. Due to the higher level
    Steponeplus Real Time Qpcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 61 article reviews
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    80
    Thermo Fisher steponeplus real time pcr system spectral calibration kit
    RNAi quantification using <t>qRT-PCR</t> and immunoblotting. A , qRT-PCR quantification of nSMase1 KD showing successful KD in nSMase1B and nSMase1C. B , qRT-PCR quantification of nSMase2 KD showing successful KD with all lentiviruses. Due to the higher level
    Steponeplus Real Time Pcr System Spectral Calibration Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 80 stars, based on 6 article reviews
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    90
    Thermo Fisher time thermal cycler
    RNAi quantification using <t>qRT-PCR</t> and immunoblotting. A , qRT-PCR quantification of nSMase1 KD showing successful KD in nSMase1B and nSMase1C. B , qRT-PCR quantification of nSMase2 KD showing successful KD with all lentiviruses. Due to the higher level
    Time Thermal Cycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 57 article reviews
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    75
    Thermo Fisher steponeplus real time qpcr instrument
    RNAi quantification using <t>qRT-PCR</t> and immunoblotting. A , qRT-PCR quantification of nSMase1 KD showing successful KD in nSMase1B and nSMase1C. B , qRT-PCR quantification of nSMase2 KD showing successful KD with all lentiviruses. Due to the higher level
    Steponeplus Real Time Qpcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 75 stars, based on 1 article reviews
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    RNAi quantification using <t>qRT-PCR</t> and immunoblotting. A , qRT-PCR quantification of nSMase1 KD showing successful KD in nSMase1B and nSMase1C. B , qRT-PCR quantification of nSMase2 KD showing successful KD with all lentiviruses. Due to the higher level
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    Immunoblot and real-time <t>PCR</t> of CA IV. A , Immunoblot of CA IV. A CA IV signal in the range of 38 kDa is present in wild-type corpus and cauda epididymidis and vas deferens. No specific CA IV band is detectable in wild-type testis and caput epididymidis or in any of the CA IV −/− tissues. B , Analysis of sperm protein fractions isolated from the different sections of the epididymidis shows a positive signal in corpus and cauda sperm and sperm from vas deferens. No specific signal is present in wild-type caput sperm or in any of the CA IV −/− sperm. C , CA IV is present in the whole vas deferens tissue and not present in the flushed vas deferens. With the luminal content only a specific CA IV band can be seen. D , kidney and brain tissue were used as positive control. E , CA IV <t>qRT</t> PCR analysis of wild-type and CA IV −/− mice. The diagram shows mean RQ values ± s.e.m. of three independent experiments for each tissue. In relation to wild-type kidney (calibrator) the RQ value of wild-type corpus epididymidis averages at 0.54. No CA IV mRNA is detectable in the other wild-type or in any of the CA IV −/− tissues (n = 3).of wild-type and CA IV −/− mice.
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    Synthetic miR-22 delivery induces cellular senescence in vivo and inhibits breast tumor growth and metastasis in vivo. (A and B) Representative fat pad orthotopic tumor (A) and selected organ (B) images of mice on day 46 after inoculation. Quantitation of bioluminescence emitted from the primary tumor (A) or whole body (B) of mice was presented as the mean ± SEM ( n = 6). L, liver; K, kidney; Sp, spleen; Si, small intestine; St, stomach. (C) Relative quantitation of miR-22 level in primary tumor tissues was analyzed by <t>qRT-PCR.</t> Total RNA was isolated from five 10-µm-thick optimal cutting temperature compound–embedded frozen tissue sections. Expression level of miR-22 in miR-22–treated tumors was relative to that in cont miR–treated tumors set at 1. U6 was used as an internal normalization control. Data represent mean ± SEM (n = 5). (D and E) Representative histochemical detection of SA-β-gal activity in vivo (D) and hematoxylin and eosin (HE) staining (E) in primary tumor on day 46 after inoculation. Images were taken with light microscopy. Seven independent fields were chosen, and the percentage of SA-β-gal–positive cells is presented in the histogram. Data represent mean ± SEM ( n = 4). Bars, 50 µm. (F) Effect of miR-22 on cell proliferation in primary tumor tissues was evaluated by Ki-67 immunostaining. The histogram displays the percentage of Ki-67 labeling index in these groups. Data represent mean ± SEM ( n = 3). *, P
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    Synthetic miR-22 delivery induces cellular senescence in vivo and inhibits breast tumor growth and metastasis in vivo. (A and B) Representative fat pad orthotopic tumor (A) and selected organ (B) images of mice on day 46 after inoculation. Quantitation of bioluminescence emitted from the primary tumor (A) or whole body (B) of mice was presented as the mean ± SEM ( n = 6). L, liver; K, kidney; Sp, spleen; Si, small intestine; St, stomach. (C) Relative quantitation of miR-22 level in primary tumor tissues was analyzed by <t>qRT-PCR.</t> Total RNA was isolated from five 10-µm-thick optimal cutting temperature compound–embedded frozen tissue sections. Expression level of miR-22 in miR-22–treated tumors was relative to that in cont miR–treated tumors set at 1. U6 was used as an internal normalization control. Data represent mean ± SEM (n = 5). (D and E) Representative histochemical detection of SA-β-gal activity in vivo (D) and hematoxylin and eosin (HE) staining (E) in primary tumor on day 46 after inoculation. Images were taken with light microscopy. Seven independent fields were chosen, and the percentage of SA-β-gal–positive cells is presented in the histogram. Data represent mean ± SEM ( n = 4). Bars, 50 µm. (F) Effect of miR-22 on cell proliferation in primary tumor tissues was evaluated by Ki-67 immunostaining. The histogram displays the percentage of Ki-67 labeling index in these groups. Data represent mean ± SEM ( n = 3). *, P
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    Image Search Results


    Analysis of mRNA and protein levels for pRb and E2F1 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of pRb and E2F1 were quantified by qRT-PCR on a StepOnePlus real-time PCR system. B. The protein levels of pRb and E2F1 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P

    Journal: Oncotarget

    Article Title: An EBV recombinant deleted for residues 130-159 in EBNA3C can deregulate p53/Mdm2 and Cyclin D1/CDK6 which results in apoptosis and reduced cell proliferation

    doi: 10.18632/oncotarget.7502

    Figure Lengend Snippet: Analysis of mRNA and protein levels for pRb and E2F1 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of pRb and E2F1 were quantified by qRT-PCR on a StepOnePlus real-time PCR system. B. The protein levels of pRb and E2F1 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P

    Article Snippet: RT-qPCR was performed on a StepOnePlus real-time PCR System (Applied Biosystems Inc, Carlsbad, CA).

    Techniques: Infection, Synthesized, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Western Blot

    Analysis of mRNA and protein levels for p53, Mdm2, CyclinD1 and Cdk6 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of p53, Mdm2, CyclinD1 and Cdk6 were quantified by qRT-PCR on a StepOnePlus real-time PCR system. B. The protein levels of p53, Mdm2, CyclinD1 and Cdk6 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P

    Journal: Oncotarget

    Article Title: An EBV recombinant deleted for residues 130-159 in EBNA3C can deregulate p53/Mdm2 and Cyclin D1/CDK6 which results in apoptosis and reduced cell proliferation

    doi: 10.18632/oncotarget.7502

    Figure Lengend Snippet: Analysis of mRNA and protein levels for p53, Mdm2, CyclinD1 and Cdk6 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of p53, Mdm2, CyclinD1 and Cdk6 were quantified by qRT-PCR on a StepOnePlus real-time PCR system. B. The protein levels of p53, Mdm2, CyclinD1 and Cdk6 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P

    Article Snippet: RT-qPCR was performed on a StepOnePlus real-time PCR System (Applied Biosystems Inc, Carlsbad, CA).

    Techniques: Infection, Synthesized, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Western Blot

    RNAi quantification using qRT-PCR and immunoblotting. A , qRT-PCR quantification of nSMase1 KD showing successful KD in nSMase1B and nSMase1C. B , qRT-PCR quantification of nSMase2 KD showing successful KD with all lentiviruses. Due to the higher level

    Journal: The Journal of Biological Chemistry

    Article Title: The Neutral Sphingomyelinase Pathway Regulates Packaging of the Prion Protein into Exosomes *

    doi: 10.1074/jbc.M114.605253

    Figure Lengend Snippet: RNAi quantification using qRT-PCR and immunoblotting. A , qRT-PCR quantification of nSMase1 KD showing successful KD in nSMase1B and nSMase1C. B , qRT-PCR quantification of nSMase2 KD showing successful KD with all lentiviruses. Due to the higher level

    Article Snippet: Samples were prepared on the QIAgility liquid-handling robot (Qiagen) and run on a StepOnePlus qRT-PCR instrument (Invitrogen) using manufacturer's recommended cycling conditions.

    Techniques: Quantitative RT-PCR

    Immunoblot and real-time PCR of CA IV. A , Immunoblot of CA IV. A CA IV signal in the range of 38 kDa is present in wild-type corpus and cauda epididymidis and vas deferens. No specific CA IV band is detectable in wild-type testis and caput epididymidis or in any of the CA IV −/− tissues. B , Analysis of sperm protein fractions isolated from the different sections of the epididymidis shows a positive signal in corpus and cauda sperm and sperm from vas deferens. No specific signal is present in wild-type caput sperm or in any of the CA IV −/− sperm. C , CA IV is present in the whole vas deferens tissue and not present in the flushed vas deferens. With the luminal content only a specific CA IV band can be seen. D , kidney and brain tissue were used as positive control. E , CA IV qRT PCR analysis of wild-type and CA IV −/− mice. The diagram shows mean RQ values ± s.e.m. of three independent experiments for each tissue. In relation to wild-type kidney (calibrator) the RQ value of wild-type corpus epididymidis averages at 0.54. No CA IV mRNA is detectable in the other wild-type or in any of the CA IV −/− tissues (n = 3).of wild-type and CA IV −/− mice.

    Journal: PLoS ONE

    Article Title: Role of Carbonic Anhydrase IV in the Bicarbonate-Mediated Activation of Murine and Human Sperm

    doi: 10.1371/journal.pone.0015061

    Figure Lengend Snippet: Immunoblot and real-time PCR of CA IV. A , Immunoblot of CA IV. A CA IV signal in the range of 38 kDa is present in wild-type corpus and cauda epididymidis and vas deferens. No specific CA IV band is detectable in wild-type testis and caput epididymidis or in any of the CA IV −/− tissues. B , Analysis of sperm protein fractions isolated from the different sections of the epididymidis shows a positive signal in corpus and cauda sperm and sperm from vas deferens. No specific signal is present in wild-type caput sperm or in any of the CA IV −/− sperm. C , CA IV is present in the whole vas deferens tissue and not present in the flushed vas deferens. With the luminal content only a specific CA IV band can be seen. D , kidney and brain tissue were used as positive control. E , CA IV qRT PCR analysis of wild-type and CA IV −/− mice. The diagram shows mean RQ values ± s.e.m. of three independent experiments for each tissue. In relation to wild-type kidney (calibrator) the RQ value of wild-type corpus epididymidis averages at 0.54. No CA IV mRNA is detectable in the other wild-type or in any of the CA IV −/− tissues (n = 3).of wild-type and CA IV −/− mice.

    Article Snippet: All measurements were carried out on a StepOnePlus™ qRT-PCR device from Applied Biosystems (Foster City, CA, USA).

    Techniques: Real-time Polymerase Chain Reaction, Isolation, Positive Control, Quantitative RT-PCR, Mouse Assay

    Synthetic miR-22 delivery induces cellular senescence in vivo and inhibits breast tumor growth and metastasis in vivo. (A and B) Representative fat pad orthotopic tumor (A) and selected organ (B) images of mice on day 46 after inoculation. Quantitation of bioluminescence emitted from the primary tumor (A) or whole body (B) of mice was presented as the mean ± SEM ( n = 6). L, liver; K, kidney; Sp, spleen; Si, small intestine; St, stomach. (C) Relative quantitation of miR-22 level in primary tumor tissues was analyzed by qRT-PCR. Total RNA was isolated from five 10-µm-thick optimal cutting temperature compound–embedded frozen tissue sections. Expression level of miR-22 in miR-22–treated tumors was relative to that in cont miR–treated tumors set at 1. U6 was used as an internal normalization control. Data represent mean ± SEM (n = 5). (D and E) Representative histochemical detection of SA-β-gal activity in vivo (D) and hematoxylin and eosin (HE) staining (E) in primary tumor on day 46 after inoculation. Images were taken with light microscopy. Seven independent fields were chosen, and the percentage of SA-β-gal–positive cells is presented in the histogram. Data represent mean ± SEM ( n = 4). Bars, 50 µm. (F) Effect of miR-22 on cell proliferation in primary tumor tissues was evaluated by Ki-67 immunostaining. The histogram displays the percentage of Ki-67 labeling index in these groups. Data represent mean ± SEM ( n = 3). *, P

    Journal: The Journal of Cell Biology

    Article Title: miR-22 represses cancer progression by inducing cellular senescence

    doi: 10.1083/jcb.201010100

    Figure Lengend Snippet: Synthetic miR-22 delivery induces cellular senescence in vivo and inhibits breast tumor growth and metastasis in vivo. (A and B) Representative fat pad orthotopic tumor (A) and selected organ (B) images of mice on day 46 after inoculation. Quantitation of bioluminescence emitted from the primary tumor (A) or whole body (B) of mice was presented as the mean ± SEM ( n = 6). L, liver; K, kidney; Sp, spleen; Si, small intestine; St, stomach. (C) Relative quantitation of miR-22 level in primary tumor tissues was analyzed by qRT-PCR. Total RNA was isolated from five 10-µm-thick optimal cutting temperature compound–embedded frozen tissue sections. Expression level of miR-22 in miR-22–treated tumors was relative to that in cont miR–treated tumors set at 1. U6 was used as an internal normalization control. Data represent mean ± SEM (n = 5). (D and E) Representative histochemical detection of SA-β-gal activity in vivo (D) and hematoxylin and eosin (HE) staining (E) in primary tumor on day 46 after inoculation. Images were taken with light microscopy. Seven independent fields were chosen, and the percentage of SA-β-gal–positive cells is presented in the histogram. Data represent mean ± SEM ( n = 4). Bars, 50 µm. (F) Effect of miR-22 on cell proliferation in primary tumor tissues was evaluated by Ki-67 immunostaining. The histogram displays the percentage of Ki-67 labeling index in these groups. Data represent mean ± SEM ( n = 3). *, P

    Article Snippet: Real-time qRT-PCR was performed using a real-time PCR system (ABI StepOne and StepOnePlus; Applied Biosystems) and LightCycle 480 (Roche).

    Techniques: In Vivo, Mouse Assay, Quantitation Assay, Quantitative RT-PCR, Isolation, Expressing, Activity Assay, Staining, Light Microscopy, Immunostaining, Labeling

    Overexpression of miR-22 induces senescence-like phenotypes in human breast epithelial and breast cancer cells. (A) qRT-PCR analysis shows relative quantitation of miR-22 expression level (vs. 184hTERT) in human epithelial and various cancer cells. Expression levels of miR-22 in various cells were relative to that in 184hTERT cells set at 1. U6 was used as an internal normalization control. The dashed line represents the threshold of expression level (0.5-fold vs. 184hTERT). (B) Cell proliferation assay was performed after transfection of miR-22, and cells were counted for the indicated days, compared with control cells. Each value was determined in triplicate. *, P

    Journal: The Journal of Cell Biology

    Article Title: miR-22 represses cancer progression by inducing cellular senescence

    doi: 10.1083/jcb.201010100

    Figure Lengend Snippet: Overexpression of miR-22 induces senescence-like phenotypes in human breast epithelial and breast cancer cells. (A) qRT-PCR analysis shows relative quantitation of miR-22 expression level (vs. 184hTERT) in human epithelial and various cancer cells. Expression levels of miR-22 in various cells were relative to that in 184hTERT cells set at 1. U6 was used as an internal normalization control. The dashed line represents the threshold of expression level (0.5-fold vs. 184hTERT). (B) Cell proliferation assay was performed after transfection of miR-22, and cells were counted for the indicated days, compared with control cells. Each value was determined in triplicate. *, P

    Article Snippet: Real-time qRT-PCR was performed using a real-time PCR system (ABI StepOne and StepOnePlus; Applied Biosystems) and LightCycle 480 (Roche).

    Techniques: Over Expression, Quantitative RT-PCR, Quantitation Assay, Expressing, Proliferation Assay, Transfection

    Overexpression of miR-22 induced cellular senescence, cell cycle G1 arrest, and the decrease in BrdU incorporation in SiHa cells. (A and B) SiHa cells were transfected with cont miR or miR-22 at the indicated concentration for 6 d. qRT-PCR results show the relative level of miR-22 expression to cont miR in each transfection group (A). SA-β-gal activity was presented by the percentage of SA-β-gal–positive cells (B). (C) Cell morphology and SA-β-gal activity were analyzed by phase-contrast microscopy at day 6 after transfection of 10 nM miR-22 or cont miR. (D) Cell proliferation assay was performed after transfection of 10 nM miR-22, and cells were counted for the indicated days, compared with control cells. Each value was determined in triplicate. **, P

    Journal: The Journal of Cell Biology

    Article Title: miR-22 represses cancer progression by inducing cellular senescence

    doi: 10.1083/jcb.201010100

    Figure Lengend Snippet: Overexpression of miR-22 induced cellular senescence, cell cycle G1 arrest, and the decrease in BrdU incorporation in SiHa cells. (A and B) SiHa cells were transfected with cont miR or miR-22 at the indicated concentration for 6 d. qRT-PCR results show the relative level of miR-22 expression to cont miR in each transfection group (A). SA-β-gal activity was presented by the percentage of SA-β-gal–positive cells (B). (C) Cell morphology and SA-β-gal activity were analyzed by phase-contrast microscopy at day 6 after transfection of 10 nM miR-22 or cont miR. (D) Cell proliferation assay was performed after transfection of 10 nM miR-22, and cells were counted for the indicated days, compared with control cells. Each value was determined in triplicate. **, P

    Article Snippet: Real-time qRT-PCR was performed using a real-time PCR system (ABI StepOne and StepOnePlus; Applied Biosystems) and LightCycle 480 (Roche).

    Techniques: Over Expression, BrdU Incorporation Assay, Transfection, Concentration Assay, Quantitative RT-PCR, Expressing, Activity Assay, Microscopy, Proliferation Assay

    miR-22 is up-regulated in senescent human fibroblasts, mediating cellular senescence. (A) miRNA expression profile of TIG-3 fibroblasts was analyzed by miRNA microarray, presented as fold changes in miRNA expression between TIG-3S (senescent) and young (Y) cells. A set of altered expression miRNAs is indicated by red columns (see Table S1 ). RQ, relative quantitation. (B) Relative quantitation of miR-22 expression in different PDLs of fibroblasts was analyzed by qRT-PCR analysis. miR-22 expression levels in human fibroblasts were indicated, relative to those in TIG-3 (42 PDL) set at 1 in the left histogram and MRC-5 (43 PDL) set at 1 in the right histogram. U6 was used as an internal normalization control. The dashed line represents the threshold of expression level (twofold vs. TIG-3 42 PDL). (C and D) MRC-5 cells were transfected with cont miR or mature miR-22 (miR-22) for 6 d at indicated concentration. (C) qRT-PCR analysis shows the relative quantitation of miR-22 expression (vs. cont miR) in each transfection group. miR-22 expression levels in miR-22–transfected MRC-5 cells were indicated, relative to that in cont miR–transfected cells set at 1. U6 was used as an internal normalization control. (D) SA-β-gal activity was presented by the percentage of SA-β-gal–positive cells, which was indicated in different dose groups. (E) Cell proliferation assay was performed after transfection of 10 nM miR-22 or cont miR, and cells were counted for the indicated days. Each value was determined in triplicate. **, P

    Journal: The Journal of Cell Biology

    Article Title: miR-22 represses cancer progression by inducing cellular senescence

    doi: 10.1083/jcb.201010100

    Figure Lengend Snippet: miR-22 is up-regulated in senescent human fibroblasts, mediating cellular senescence. (A) miRNA expression profile of TIG-3 fibroblasts was analyzed by miRNA microarray, presented as fold changes in miRNA expression between TIG-3S (senescent) and young (Y) cells. A set of altered expression miRNAs is indicated by red columns (see Table S1 ). RQ, relative quantitation. (B) Relative quantitation of miR-22 expression in different PDLs of fibroblasts was analyzed by qRT-PCR analysis. miR-22 expression levels in human fibroblasts were indicated, relative to those in TIG-3 (42 PDL) set at 1 in the left histogram and MRC-5 (43 PDL) set at 1 in the right histogram. U6 was used as an internal normalization control. The dashed line represents the threshold of expression level (twofold vs. TIG-3 42 PDL). (C and D) MRC-5 cells were transfected with cont miR or mature miR-22 (miR-22) for 6 d at indicated concentration. (C) qRT-PCR analysis shows the relative quantitation of miR-22 expression (vs. cont miR) in each transfection group. miR-22 expression levels in miR-22–transfected MRC-5 cells were indicated, relative to that in cont miR–transfected cells set at 1. U6 was used as an internal normalization control. (D) SA-β-gal activity was presented by the percentage of SA-β-gal–positive cells, which was indicated in different dose groups. (E) Cell proliferation assay was performed after transfection of 10 nM miR-22 or cont miR, and cells were counted for the indicated days. Each value was determined in triplicate. **, P

    Article Snippet: Real-time qRT-PCR was performed using a real-time PCR system (ABI StepOne and StepOnePlus; Applied Biosystems) and LightCycle 480 (Roche).

    Techniques: Expressing, Microarray, Quantitation Assay, Quantitative RT-PCR, Transfection, Concentration Assay, Activity Assay, Proliferation Assay