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    New England Biolabs new england biolabs taq buffer
    New England Biolabs Taq Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 208 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/new england biolabs taq buffer/product/New England Biolabs
    Average 99 stars, based on 208 article reviews
    Price from $9.99 to $1999.99
    new england biolabs taq buffer - by Bioz Stars, 2020-05
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    New England Biolabs new england biolabs taq polymerase
    Single strand binding protein, ET SSB, only has a minor effect on the reduction of artifacts. <t>Taq</t> (NE Biolabs) and AccuPrime Pfx (Life Technologies) <t>DNA</t> polymerases were used in amplification of TALE DNA repeats. The arrows indicate the expected size of the amplification products. PCR conditions are given in the supplementary material .
    New England Biolabs Taq Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/new england biolabs taq polymerase/product/New England Biolabs
    Average 99 stars, based on 80 article reviews
    Price from $9.99 to $1999.99
    new england biolabs taq polymerase - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    93
    New England Biolabs taq mg free buffer
    Single strand binding protein, ET SSB, only has a minor effect on the reduction of artifacts. <t>Taq</t> (NE Biolabs) and AccuPrime Pfx (Life Technologies) <t>DNA</t> polymerases were used in amplification of TALE DNA repeats. The arrows indicate the expected size of the amplification products. PCR conditions are given in the supplementary material .
    Taq Mg Free Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq mg free buffer/product/New England Biolabs
    Average 93 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    taq mg free buffer - by Bioz Stars, 2020-05
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    New England Biolabs thermopol taq reaction buffer
    Single strand binding protein, ET SSB, only has a minor effect on the reduction of artifacts. <t>Taq</t> (NE Biolabs) and AccuPrime Pfx (Life Technologies) <t>DNA</t> polymerases were used in amplification of TALE DNA repeats. The arrows indicate the expected size of the amplification products. PCR conditions are given in the supplementary material .
    Thermopol Taq Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermopol taq reaction buffer/product/New England Biolabs
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    thermopol taq reaction buffer - by Bioz Stars, 2020-05
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      Buy from Supplier

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    Single strand binding protein, ET SSB, only has a minor effect on the reduction of artifacts. Taq (NE Biolabs) and AccuPrime Pfx (Life Technologies) DNA polymerases were used in amplification of TALE DNA repeats. The arrows indicate the expected size of the amplification products. PCR conditions are given in the supplementary material .

    Journal: Scientific Reports

    Article Title: PCR amplification of repetitive DNA: a limitation to genome editing technologies and many other applications

    doi: 10.1038/srep05052

    Figure Lengend Snippet: Single strand binding protein, ET SSB, only has a minor effect on the reduction of artifacts. Taq (NE Biolabs) and AccuPrime Pfx (Life Technologies) DNA polymerases were used in amplification of TALE DNA repeats. The arrows indicate the expected size of the amplification products. PCR conditions are given in the supplementary material .

    Article Snippet: Even the use of a non-proofreading but robust DNA polymerase, Taq (NE Biolabs) failed to amplify only the desired fragment ( ).

    Techniques: Binding Assay, Amplification, Polymerase Chain Reaction

    Primers that anneal far away from the repetitive DNA perform much better in amplifying the desired product. Taq DNA polymerase (NE Biolabs) was used in the PCR amplification of the indicated region of the pdTALE12 plasmid. PCR conditions are given in the supplementary material .

    Journal: Scientific Reports

    Article Title: PCR amplification of repetitive DNA: a limitation to genome editing technologies and many other applications

    doi: 10.1038/srep05052

    Figure Lengend Snippet: Primers that anneal far away from the repetitive DNA perform much better in amplifying the desired product. Taq DNA polymerase (NE Biolabs) was used in the PCR amplification of the indicated region of the pdTALE12 plasmid. PCR conditions are given in the supplementary material .

    Article Snippet: Even the use of a non-proofreading but robust DNA polymerase, Taq (NE Biolabs) failed to amplify only the desired fragment ( ).

    Techniques: Polymerase Chain Reaction, Amplification, Plasmid Preparation

    PCR fragments generated upon a typical PCR amplification from the pTAL2 vector with 12.5 TALE DNA-binding repeats. Plasmid map is shown in Supplementary Fig. 3 . Proofreading Pfu polymerase (Bioline) and normal Taq polymerase (NE Biolabs) were used in PCR amplification. PCR conditions are described in the supplementary material .

    Journal: Scientific Reports

    Article Title: PCR amplification of repetitive DNA: a limitation to genome editing technologies and many other applications

    doi: 10.1038/srep05052

    Figure Lengend Snippet: PCR fragments generated upon a typical PCR amplification from the pTAL2 vector with 12.5 TALE DNA-binding repeats. Plasmid map is shown in Supplementary Fig. 3 . Proofreading Pfu polymerase (Bioline) and normal Taq polymerase (NE Biolabs) were used in PCR amplification. PCR conditions are described in the supplementary material .

    Article Snippet: Even the use of a non-proofreading but robust DNA polymerase, Taq (NE Biolabs) failed to amplify only the desired fragment ( ).

    Techniques: Polymerase Chain Reaction, Generated, Amplification, Plasmid Preparation, Binding Assay

    Testing the generality of the model to other template DNAs with repetitive sequences. (A). GFP-coding sequences were cloned into the pBasicS1 vector and their integrity were checked by sequencing and restriction enzyme digestions. (B). PCR results obtained with primers 390 and 570. Taq DNA polymerase (NE Biolabs) was used in PCR amplification. The arrow indicates the sequenced artifact product which contained only one copy of the GFP lacking the filler sequence. See the supplementary material for PCR conditions.

    Journal: Scientific Reports

    Article Title: PCR amplification of repetitive DNA: a limitation to genome editing technologies and many other applications

    doi: 10.1038/srep05052

    Figure Lengend Snippet: Testing the generality of the model to other template DNAs with repetitive sequences. (A). GFP-coding sequences were cloned into the pBasicS1 vector and their integrity were checked by sequencing and restriction enzyme digestions. (B). PCR results obtained with primers 390 and 570. Taq DNA polymerase (NE Biolabs) was used in PCR amplification. The arrow indicates the sequenced artifact product which contained only one copy of the GFP lacking the filler sequence. See the supplementary material for PCR conditions.

    Article Snippet: Even the use of a non-proofreading but robust DNA polymerase, Taq (NE Biolabs) failed to amplify only the desired fragment ( ).

    Techniques: Clone Assay, Plasmid Preparation, Sequencing, Polymerase Chain Reaction, Amplification

    PCR products of UBP4′: M-marker (bp) GeneRuler DNA Ladder Mix (Fermentas Life Sciences), lanes 1–8 UBP4′ gene (276 bp UBP4′ length and 26 bp for restriction enzymes sequence = 302 bp) PCR product: lanes 1, 2: PCR reaction with Biotools DNA polymerase and 23 cycles, lanes 3, 4: PCR reaction with Biotools DNA polymerase and 29 cycles, lanes 5, 6: PCR reaction with Biotools DNA polymerase and 29 cycles, and lanes 7, 8: PCR reaction with Taq DNA polymerase and 29 cycles.

    Journal: BioMed Research International

    Article Title: DNASynth: A Computer Program for Assembly of Artificial Gene Parts in Decreasing Temperature

    doi: 10.1155/2015/413262

    Figure Lengend Snippet: PCR products of UBP4′: M-marker (bp) GeneRuler DNA Ladder Mix (Fermentas Life Sciences), lanes 1–8 UBP4′ gene (276 bp UBP4′ length and 26 bp for restriction enzymes sequence = 302 bp) PCR product: lanes 1, 2: PCR reaction with Biotools DNA polymerase and 23 cycles, lanes 3, 4: PCR reaction with Biotools DNA polymerase and 29 cycles, lanes 5, 6: PCR reaction with Biotools DNA polymerase and 29 cycles, and lanes 7, 8: PCR reaction with Taq DNA polymerase and 29 cycles.

    Article Snippet: S.A. or Taq DNA polymerase with standard Taq buffer, New England Biolabs, Inc.), and 1 μ L as a template for 23 and 29 cycles using Eppendorf 5330 thermocycler.

    Techniques: Polymerase Chain Reaction, Marker, Sequencing