ssi beads Search Results


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  • 99
    Millipore anti socs3 shrna
    Anti Socs3 Shrna, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Santa Cruz Biotechnology socs3 antibody
    Reduced cell viability in response to TRAIL depends on <t>SOCS3</t> expression Cell viability after treatment with IFN-α and/or TRAIL were compared with non-treated cells. (A) ACHN cells seeded on a culture plate were transfected with an empty vector or pCI-SOCS3 vector. (B) 786-O cells were transfected with negative control siRNA or SOCS3 siRNA. Cell viability after TRAIL treatment changed in a SOCS3-dependent manner. * p
    Socs3 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Tecan Systems anti socs3
    Reduced cell viability in response to TRAIL depends on <t>SOCS3</t> expression Cell viability after treatment with IFN-α and/or TRAIL were compared with non-treated cells. (A) ACHN cells seeded on a culture plate were transfected with an empty vector or pCI-SOCS3 vector. (B) 786-O cells were transfected with negative control siRNA or SOCS3 siRNA. Cell viability after TRAIL treatment changed in a SOCS3-dependent manner. * p
    Anti Socs3, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Promega adp glo kinase assay kit
    Reduced cell viability in response to TRAIL depends on <t>SOCS3</t> expression Cell viability after treatment with IFN-α and/or TRAIL were compared with non-treated cells. (A) ACHN cells seeded on a culture plate were transfected with an empty vector or pCI-SOCS3 vector. (B) 786-O cells were transfected with negative control siRNA or SOCS3 siRNA. Cell viability after TRAIL treatment changed in a SOCS3-dependent manner. * p
    Adp Glo Kinase Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 95/100, based on 531 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Luminex in house luminex bead based assay
    Reduced cell viability in response to TRAIL depends on <t>SOCS3</t> expression Cell viability after treatment with IFN-α and/or TRAIL were compared with non-treated cells. (A) ACHN cells seeded on a culture plate were transfected with an empty vector or pCI-SOCS3 vector. (B) 786-O cells were transfected with negative control siRNA or SOCS3 siRNA. Cell viability after TRAIL treatment changed in a SOCS3-dependent manner. * p
    In House Luminex Bead Based Assay, supplied by Luminex, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Abcam mouse anti socs3
    <t>SOCS3</t> knockdown increases HCV replication in JFH1-infected cells. Stable knockdown by shRNA targeting SOCS3 (shSOCS3) in Huh7.5.1 cells was established as described in Materials and Methods. We infected cells with inoculum from JFH1-infected Huh7.5.1
    Mouse Anti Socs3, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Qiagen powersoil dna kit bead solution
    <t>SOCS3</t> knockdown increases HCV replication in JFH1-infected cells. Stable knockdown by shRNA targeting SOCS3 (shSOCS3) in Huh7.5.1 cells was established as described in Materials and Methods. We infected cells with inoculum from JFH1-infected Huh7.5.1
    Powersoil Dna Kit Bead Solution, supplied by Qiagen, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Santa Cruz Biotechnology socs3
    Loss of <t>SOCS3</t> in CD4 + T cells promotes the expansion of regulatory T cells and their suppressive activities. (A) Naïve CD4 + T cells were isolated and purified from lymph nodes and spleen of WT or CD4-SOCS3KO mice and stimulated with anti-CD3/CD28
    Socs3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 706 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    ATCC capsular pn ps
    Loss of <t>SOCS3</t> in CD4 + T cells promotes the expansion of regulatory T cells and their suppressive activities. (A) Naïve CD4 + T cells were isolated and purified from lymph nodes and spleen of WT or CD4-SOCS3KO mice and stimulated with anti-CD3/CD28
    Capsular Pn Ps, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Thermo Fisher nanodrop spectrophotomer
    Loss of <t>SOCS3</t> in CD4 + T cells promotes the expansion of regulatory T cells and their suppressive activities. (A) Naïve CD4 + T cells were isolated and purified from lymph nodes and spleen of WT or CD4-SOCS3KO mice and stimulated with anti-CD3/CD28
    Nanodrop Spectrophotomer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Bio-Rad affi gel 10
    Loss of <t>SOCS3</t> in CD4 + T cells promotes the expansion of regulatory T cells and their suppressive activities. (A) Naïve CD4 + T cells were isolated and purified from lymph nodes and spleen of WT or CD4-SOCS3KO mice and stimulated with anti-CD3/CD28
    Affi Gel 10, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1370 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    SSI Diagnostica ssi diagnostica polysaccharides
    Loss of <t>SOCS3</t> in CD4 + T cells promotes the expansion of regulatory T cells and their suppressive activities. (A) Naïve CD4 + T cells were isolated and purified from lymph nodes and spleen of WT or CD4-SOCS3KO mice and stimulated with anti-CD3/CD28
    Ssi Diagnostica Polysaccharides, supplied by SSI Diagnostica, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Luminex microsphere flow cytometric
    Loss of <t>SOCS3</t> in CD4 + T cells promotes the expansion of regulatory T cells and their suppressive activities. (A) Naïve CD4 + T cells were isolated and purified from lymph nodes and spleen of WT or CD4-SOCS3KO mice and stimulated with anti-CD3/CD28
    Microsphere Flow Cytometric, supplied by Luminex, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Luminex luminex beads protein
    Antibody responces in vaccinated mice. A shows the IgG antibody responces to the carrier protein AcrA in vaccinated mice, measured using a <t>luminex</t> assay. B shows the IgG antibody responces to type 4 polysaccharide in vaccinated mice, measured using a Luminex assay. Antibody responces were measured in sera taken pre challenge with TIGR4. Each dot represent antibody levels in a single animal. Red circular symbol indicates the antibody levels in the mouse that was vaccinated with AcrA-SP4 P2 and survived challenge. Statistical analysis was performed in GraphPad Prism using a Kruskal-Wallis one way ANOVA with a Dunn’s multiple comparison test, *P
    Luminex Beads Protein, supplied by Luminex, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    GE Healthcare g25 pd 10 sephadex column
    Antibody responces in vaccinated mice. A shows the IgG antibody responces to the carrier protein AcrA in vaccinated mice, measured using a <t>luminex</t> assay. B shows the IgG antibody responces to type 4 polysaccharide in vaccinated mice, measured using a Luminex assay. Antibody responces were measured in sera taken pre challenge with TIGR4. Each dot represent antibody levels in a single animal. Red circular symbol indicates the antibody levels in the mouse that was vaccinated with AcrA-SP4 P2 and survived challenge. Statistical analysis was performed in GraphPad Prism using a Kruskal-Wallis one way ANOVA with a Dunn’s multiple comparison test, *P
    G25 Pd 10 Sephadex Column, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Luminex luminex luminometer
    Antibody responces in vaccinated mice. A shows the IgG antibody responces to the carrier protein AcrA in vaccinated mice, measured using a <t>luminex</t> assay. B shows the IgG antibody responces to type 4 polysaccharide in vaccinated mice, measured using a Luminex assay. Antibody responces were measured in sera taken pre challenge with TIGR4. Each dot represent antibody levels in a single animal. Red circular symbol indicates the antibody levels in the mouse that was vaccinated with AcrA-SP4 P2 and survived challenge. Statistical analysis was performed in GraphPad Prism using a Kruskal-Wallis one way ANOVA with a Dunn’s multiple comparison test, *P
    Luminex Luminometer, supplied by Luminex, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Bio-Rad polyprep chromatography column
    Antibody responces in vaccinated mice. A shows the IgG antibody responces to the carrier protein AcrA in vaccinated mice, measured using a <t>luminex</t> assay. B shows the IgG antibody responces to type 4 polysaccharide in vaccinated mice, measured using a Luminex assay. Antibody responces were measured in sera taken pre challenge with TIGR4. Each dot represent antibody levels in a single animal. Red circular symbol indicates the antibody levels in the mouse that was vaccinated with AcrA-SP4 P2 and survived challenge. Statistical analysis was performed in GraphPad Prism using a Kruskal-Wallis one way ANOVA with a Dunn’s multiple comparison test, *P
    Polyprep Chromatography Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 206 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Luminex luminex multiplex machine
    Antibody responces in vaccinated mice. A shows the IgG antibody responces to the carrier protein AcrA in vaccinated mice, measured using a <t>luminex</t> assay. B shows the IgG antibody responces to type 4 polysaccharide in vaccinated mice, measured using a Luminex assay. Antibody responces were measured in sera taken pre challenge with TIGR4. Each dot represent antibody levels in a single animal. Red circular symbol indicates the antibody levels in the mouse that was vaccinated with AcrA-SP4 P2 and survived challenge. Statistical analysis was performed in GraphPad Prism using a Kruskal-Wallis one way ANOVA with a Dunn’s multiple comparison test, *P
    Luminex Multiplex Machine, supplied by Luminex, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Beckman Coulter gallios navios
    Antibody responces in vaccinated mice. A shows the IgG antibody responces to the carrier protein AcrA in vaccinated mice, measured using a <t>luminex</t> assay. B shows the IgG antibody responces to type 4 polysaccharide in vaccinated mice, measured using a Luminex assay. Antibody responces were measured in sera taken pre challenge with TIGR4. Each dot represent antibody levels in a single animal. Red circular symbol indicates the antibody levels in the mouse that was vaccinated with AcrA-SP4 P2 and survived challenge. Statistical analysis was performed in GraphPad Prism using a Kruskal-Wallis one way ANOVA with a Dunn’s multiple comparison test, *P
    Gallios Navios, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Cell Signaling Technology Inc anti socs3 ab
    Antibody responces in vaccinated mice. A shows the IgG antibody responces to the carrier protein AcrA in vaccinated mice, measured using a <t>luminex</t> assay. B shows the IgG antibody responces to type 4 polysaccharide in vaccinated mice, measured using a Luminex assay. Antibody responces were measured in sera taken pre challenge with TIGR4. Each dot represent antibody levels in a single animal. Red circular symbol indicates the antibody levels in the mouse that was vaccinated with AcrA-SP4 P2 and survived challenge. Statistical analysis was performed in GraphPad Prism using a Kruskal-Wallis one way ANOVA with a Dunn’s multiple comparison test, *P
    Anti Socs3 Ab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Luminex luminex 100 200 platform
    Antibody responces in vaccinated mice. A shows the IgG antibody responces to the carrier protein AcrA in vaccinated mice, measured using a <t>luminex</t> assay. B shows the IgG antibody responces to type 4 polysaccharide in vaccinated mice, measured using a Luminex assay. Antibody responces were measured in sera taken pre challenge with TIGR4. Each dot represent antibody levels in a single animal. Red circular symbol indicates the antibody levels in the mouse that was vaccinated with AcrA-SP4 P2 and survived challenge. Statistical analysis was performed in GraphPad Prism using a Kruskal-Wallis one way ANOVA with a Dunn’s multiple comparison test, *P
    Luminex 100 200 Platform, supplied by Luminex, used in various techniques. Bioz Stars score: 93/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher polyclonal goat anti rabbit r phycoerythrin rpe conjugate solution
    Antibody responces in vaccinated mice. A shows the IgG antibody responces to the carrier protein AcrA in vaccinated mice, measured using a <t>luminex</t> assay. B shows the IgG antibody responces to type 4 polysaccharide in vaccinated mice, measured using a Luminex assay. Antibody responces were measured in sera taken pre challenge with TIGR4. Each dot represent antibody levels in a single animal. Red circular symbol indicates the antibody levels in the mouse that was vaccinated with AcrA-SP4 P2 and survived challenge. Statistical analysis was performed in GraphPad Prism using a Kruskal-Wallis one way ANOVA with a Dunn’s multiple comparison test, *P
    Polyclonal Goat Anti Rabbit R Phycoerythrin Rpe Conjugate Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Cell Signaling Technology Inc rabbit anti socs3
    CD37 controls the IL-6 pathway via <t>SOCS3,</t> and lymphoma development is dependent on IL-6 in vivo.
    Rabbit Anti Socs3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Santa Cruz Biotechnology socs3 goat polyclonal antibody
    PDGF-mediated <t>SOCS3</t> induction in human corneal fibroblasts involves JAK2-STAT3 pathway. Human corneal fibroblasts were exposed to a mixture of PDGF (AA, AB, and BB) at a final concentration of 20 ng/ml with or without JAK2-STAT3 inhibitor, AG-490 (final concentration of 100 μM) for 1 h or 8 h. SOCS3 transcript levels were quantified by real-time PCR, and protein levels were determined by immunoprecipitation and immunoblotting using the SOCS3 <t>polyclonal</t> antibody. A : The fold change in SOCS3 transcript levels compared to untreated control is shown as mean ±SEM. The PDGF treatment increases SOCS3 mRNA levels by threefold at 1 h and 4.5 fold at 8 h, and AG-490 treatment inhibits PDGF-induced SOCS3 mRNA levels significantly. The asterisk indicates a p
    Socs3 Goat Polyclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    ATCC msphere 00455 19 1 text s1 batch numbers
    PDGF-mediated <t>SOCS3</t> induction in human corneal fibroblasts involves JAK2-STAT3 pathway. Human corneal fibroblasts were exposed to a mixture of PDGF (AA, AB, and BB) at a final concentration of 20 ng/ml with or without JAK2-STAT3 inhibitor, AG-490 (final concentration of 100 μM) for 1 h or 8 h. SOCS3 transcript levels were quantified by real-time PCR, and protein levels were determined by immunoprecipitation and immunoblotting using the SOCS3 <t>polyclonal</t> antibody. A : The fold change in SOCS3 transcript levels compared to untreated control is shown as mean ±SEM. The PDGF treatment increases SOCS3 mRNA levels by threefold at 1 h and 4.5 fold at 8 h, and AG-490 treatment inhibits PDGF-induced SOCS3 mRNA levels significantly. The asterisk indicates a p
    Msphere 00455 19 1 Text S1 Batch Numbers, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Santa Cruz Biotechnology murine anti socs3 antibody
    A , co-immunoprecipiation of endogenous <t>SOCS3</t> and Brk. T47D breast cancer cell lysates were immunoprecipitated ( IP ) with control rabbit immunoglobulin ( cIg ) or rabbit anti-Brk. Western blots ( WB ) were performed with murine antibody to SOCS3 or Brk. Molecular
    Murine Anti Socs3 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Reduced cell viability in response to TRAIL depends on SOCS3 expression Cell viability after treatment with IFN-α and/or TRAIL were compared with non-treated cells. (A) ACHN cells seeded on a culture plate were transfected with an empty vector or pCI-SOCS3 vector. (B) 786-O cells were transfected with negative control siRNA or SOCS3 siRNA. Cell viability after TRAIL treatment changed in a SOCS3-dependent manner. * p

    Journal: Oncotarget

    Article Title: Suppression of SOCS3 enhances TRAIL-induced cell growth inhibition through the upregulation of DR4 expression in renal cell carcinoma cells

    doi: 10.18632/oncotarget.25851

    Figure Lengend Snippet: Reduced cell viability in response to TRAIL depends on SOCS3 expression Cell viability after treatment with IFN-α and/or TRAIL were compared with non-treated cells. (A) ACHN cells seeded on a culture plate were transfected with an empty vector or pCI-SOCS3 vector. (B) 786-O cells were transfected with negative control siRNA or SOCS3 siRNA. Cell viability after TRAIL treatment changed in a SOCS3-dependent manner. * p

    Article Snippet: SOCS3 antibody (Santa Cruz Biotechnology, Inc., TX) and Protein G were used to make the antibody-coated beads.

    Techniques: Expressing, Transfection, Plasmid Preparation, Negative Control

    Combination of IFN-α and tocilizumab lead the inhibition of cell viability in response to TRAIL Antihuman IL-6 receptor antibody, tocilizumab, was used for the inhibition of IL-6 signaling 786-O cells were treated with IFN-α and/or tocilizumab (50 μg/mL). (A) Effect of IFN-α and tocilizumab on mRNA expression of DR4 and SOCS3. (B) Effect of IFN-α and tocilizumab on the protein expression of DR4 and SOCS3. (C) 786-O cells were treated with IFN-α, tocilizumab and/or TRAIL. IFN-α treatment with SOCS3 suppression increased DR4 protein expression. Combined use of IFN-α, tocilizumab and TRAIL markedly inhibited cell growth. The results are expressed as the relative mean ratio ± SE of at least three independent determinations. * p

    Journal: Oncotarget

    Article Title: Suppression of SOCS3 enhances TRAIL-induced cell growth inhibition through the upregulation of DR4 expression in renal cell carcinoma cells

    doi: 10.18632/oncotarget.25851

    Figure Lengend Snippet: Combination of IFN-α and tocilizumab lead the inhibition of cell viability in response to TRAIL Antihuman IL-6 receptor antibody, tocilizumab, was used for the inhibition of IL-6 signaling 786-O cells were treated with IFN-α and/or tocilizumab (50 μg/mL). (A) Effect of IFN-α and tocilizumab on mRNA expression of DR4 and SOCS3. (B) Effect of IFN-α and tocilizumab on the protein expression of DR4 and SOCS3. (C) 786-O cells were treated with IFN-α, tocilizumab and/or TRAIL. IFN-α treatment with SOCS3 suppression increased DR4 protein expression. Combined use of IFN-α, tocilizumab and TRAIL markedly inhibited cell growth. The results are expressed as the relative mean ratio ± SE of at least three independent determinations. * p

    Article Snippet: SOCS3 antibody (Santa Cruz Biotechnology, Inc., TX) and Protein G were used to make the antibody-coated beads.

    Techniques: Inhibition, Expressing

    Interferon (IFN)-α-induced mRNA expression of TRAIL-R1/DR4, TRAIL-R2/DR5 and suppressor of cytokine signaling 3 (SOCS3) in RCC cells mRNA expression levels of DR4, DR5 and SOCS3 were quantified by real-time polymerase chain reaction (PCR) in ACHN and 786-O cells. The y axis shows the relative mRNA expression level with or without IFN-α treatment (1000 IU/mL) relative to that in non-treated ACHN cells. The results are expressed as the relative mean ratio ± SE of at least three independent determinations. * p

    Journal: Oncotarget

    Article Title: Suppression of SOCS3 enhances TRAIL-induced cell growth inhibition through the upregulation of DR4 expression in renal cell carcinoma cells

    doi: 10.18632/oncotarget.25851

    Figure Lengend Snippet: Interferon (IFN)-α-induced mRNA expression of TRAIL-R1/DR4, TRAIL-R2/DR5 and suppressor of cytokine signaling 3 (SOCS3) in RCC cells mRNA expression levels of DR4, DR5 and SOCS3 were quantified by real-time polymerase chain reaction (PCR) in ACHN and 786-O cells. The y axis shows the relative mRNA expression level with or without IFN-α treatment (1000 IU/mL) relative to that in non-treated ACHN cells. The results are expressed as the relative mean ratio ± SE of at least three independent determinations. * p

    Article Snippet: SOCS3 antibody (Santa Cruz Biotechnology, Inc., TX) and Protein G were used to make the antibody-coated beads.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction

    Effect of pCI-SOCS3 vector or SOCS3 siRNA transfection on DR4 mRNA expression mRNA expression levels of DR4, DR5 and SOCS3 were quantified by real-time PCR. DR4 mRNA expression was increased by IFN-α stimulation but not by SOCS3. The y axis shows the relative mRNA expression level. The results are expressed as the relative mean ratio ± SE of at least three independent determinations. * p

    Journal: Oncotarget

    Article Title: Suppression of SOCS3 enhances TRAIL-induced cell growth inhibition through the upregulation of DR4 expression in renal cell carcinoma cells

    doi: 10.18632/oncotarget.25851

    Figure Lengend Snippet: Effect of pCI-SOCS3 vector or SOCS3 siRNA transfection on DR4 mRNA expression mRNA expression levels of DR4, DR5 and SOCS3 were quantified by real-time PCR. DR4 mRNA expression was increased by IFN-α stimulation but not by SOCS3. The y axis shows the relative mRNA expression level. The results are expressed as the relative mean ratio ± SE of at least three independent determinations. * p

    Article Snippet: SOCS3 antibody (Santa Cruz Biotechnology, Inc., TX) and Protein G were used to make the antibody-coated beads.

    Techniques: Plasmid Preparation, Transfection, Expressing, Real-time Polymerase Chain Reaction

    DR4 protein expression depends on SOCS3 Individual bands of the Western blotting analysis were quantified using Image Lab 3.0 software and compared with protein expression levels. (A) Effect of pCI-SOCS3 on ACHN cells. Overexpression of SOCS3 suppressed the DR4 expression by IFN in ACHN cells. (B) Effect of SOCS3-siRNA on 786-O cells. DR4 protein expression was dependent on SOCS3 expression. The results are expressed as the relative mean ratio ± SE of at least three independent determinations. * p

    Journal: Oncotarget

    Article Title: Suppression of SOCS3 enhances TRAIL-induced cell growth inhibition through the upregulation of DR4 expression in renal cell carcinoma cells

    doi: 10.18632/oncotarget.25851

    Figure Lengend Snippet: DR4 protein expression depends on SOCS3 Individual bands of the Western blotting analysis were quantified using Image Lab 3.0 software and compared with protein expression levels. (A) Effect of pCI-SOCS3 on ACHN cells. Overexpression of SOCS3 suppressed the DR4 expression by IFN in ACHN cells. (B) Effect of SOCS3-siRNA on 786-O cells. DR4 protein expression was dependent on SOCS3 expression. The results are expressed as the relative mean ratio ± SE of at least three independent determinations. * p

    Article Snippet: SOCS3 antibody (Santa Cruz Biotechnology, Inc., TX) and Protein G were used to make the antibody-coated beads.

    Techniques: Expressing, Western Blot, Software, Over Expression

    SOCS3 binds to DR4 protein We investigated the biochemical interaction between SOCS-3 and DR-4 by co-immunoprecipitation. Cells were treated with 1000 IU/mL of IFN-α for different time periods. Cells were then dissolved in lysis buffer and proteins were immunoprecipitated with anti-SOCS3 antibody. Cell lysates and immunoprecipitants were applied to SDS-PAGE and Western blotting analyses were carried out with anti-SOCS3 and anti-DR4 antibodies.

    Journal: Oncotarget

    Article Title: Suppression of SOCS3 enhances TRAIL-induced cell growth inhibition through the upregulation of DR4 expression in renal cell carcinoma cells

    doi: 10.18632/oncotarget.25851

    Figure Lengend Snippet: SOCS3 binds to DR4 protein We investigated the biochemical interaction between SOCS-3 and DR-4 by co-immunoprecipitation. Cells were treated with 1000 IU/mL of IFN-α for different time periods. Cells were then dissolved in lysis buffer and proteins were immunoprecipitated with anti-SOCS3 antibody. Cell lysates and immunoprecipitants were applied to SDS-PAGE and Western blotting analyses were carried out with anti-SOCS3 and anti-DR4 antibodies.

    Article Snippet: SOCS3 antibody (Santa Cruz Biotechnology, Inc., TX) and Protein G were used to make the antibody-coated beads.

    Techniques: Immunoprecipitation, Lysis, SDS Page, Western Blot

    SOCS3 knockdown increases HCV replication in JFH1-infected cells. Stable knockdown by shRNA targeting SOCS3 (shSOCS3) in Huh7.5.1 cells was established as described in Materials and Methods. We infected cells with inoculum from JFH1-infected Huh7.5.1

    Journal: Journal of Virology

    Article Title: Suppressor of Cytokine Signaling 3 Suppresses Hepatitis C Virus Replication in an mTOR-Dependent Manner ▿

    doi: 10.1128/JVI.02484-09

    Figure Lengend Snippet: SOCS3 knockdown increases HCV replication in JFH1-infected cells. Stable knockdown by shRNA targeting SOCS3 (shSOCS3) in Huh7.5.1 cells was established as described in Materials and Methods. We infected cells with inoculum from JFH1-infected Huh7.5.1

    Article Snippet: Protein A/G beads were incubated with mouse anti-SOCS3 (Abcam, Inc., Cambridge, MA) at 4°C with rolling for 2 h. After incubation, the beads were washed three times with phosphate-buffered saline (PBS).

    Techniques: Infection, shRNA

    SOCS3 downregulates HCV replication in JFH1-infected cells. Twenty-four hours after transfection with the pCDNA3-Myc or pCDNA3-Myc-hSOCS3 plasmid, Huh7.5.1 cells were infected with JFH1. At 72 h postinfection, Western blotting and qPCR were performed

    Journal: Journal of Virology

    Article Title: Suppressor of Cytokine Signaling 3 Suppresses Hepatitis C Virus Replication in an mTOR-Dependent Manner ▿

    doi: 10.1128/JVI.02484-09

    Figure Lengend Snippet: SOCS3 downregulates HCV replication in JFH1-infected cells. Twenty-four hours after transfection with the pCDNA3-Myc or pCDNA3-Myc-hSOCS3 plasmid, Huh7.5.1 cells were infected with JFH1. At 72 h postinfection, Western blotting and qPCR were performed

    Article Snippet: Protein A/G beads were incubated with mouse anti-SOCS3 (Abcam, Inc., Cambridge, MA) at 4°C with rolling for 2 h. After incubation, the beads were washed three times with phosphate-buffered saline (PBS).

    Techniques: Infection, Transfection, Plasmid Preparation, Western Blot, Real-time Polymerase Chain Reaction

    SOCS3 is degraded in a ubiquitination- and proteasome-dependent manner. Huh7.5.1 cells were infected with JFH1 virus or no-JFH1 virus mock infection medium. At days 1, 2, 3, 5, 7, 14, 21, and 28 postinfection, cells were collected, total RNA was isolated,

    Journal: Journal of Virology

    Article Title: Suppressor of Cytokine Signaling 3 Suppresses Hepatitis C Virus Replication in an mTOR-Dependent Manner ▿

    doi: 10.1128/JVI.02484-09

    Figure Lengend Snippet: SOCS3 is degraded in a ubiquitination- and proteasome-dependent manner. Huh7.5.1 cells were infected with JFH1 virus or no-JFH1 virus mock infection medium. At days 1, 2, 3, 5, 7, 14, 21, and 28 postinfection, cells were collected, total RNA was isolated,

    Article Snippet: Protein A/G beads were incubated with mouse anti-SOCS3 (Abcam, Inc., Cambridge, MA) at 4°C with rolling for 2 h. After incubation, the beads were washed three times with phosphate-buffered saline (PBS).

    Techniques: Infection, Isolation

    mTOR inhibition reverses the inhibitory effects of SOCS3 on HCV replication in JFH1-infected cells. Huh7.5.1 cells were transfected with the pCDNA3-Myc or pCDNA3-Myc-hSOCS3 plasmid. Twenty-four hours after transfection, the cells were mock infected or

    Journal: Journal of Virology

    Article Title: Suppressor of Cytokine Signaling 3 Suppresses Hepatitis C Virus Replication in an mTOR-Dependent Manner ▿

    doi: 10.1128/JVI.02484-09

    Figure Lengend Snippet: mTOR inhibition reverses the inhibitory effects of SOCS3 on HCV replication in JFH1-infected cells. Huh7.5.1 cells were transfected with the pCDNA3-Myc or pCDNA3-Myc-hSOCS3 plasmid. Twenty-four hours after transfection, the cells were mock infected or

    Article Snippet: Protein A/G beads were incubated with mouse anti-SOCS3 (Abcam, Inc., Cambridge, MA) at 4°C with rolling for 2 h. After incubation, the beads were washed three times with phosphate-buffered saline (PBS).

    Techniques: Inhibition, Infection, Transfection, Plasmid Preparation

    HCV replication decreases SOCS3 protein expression. Huh7 cells, OR6 cells cured of the HCV replicon, OR6 cells, Huh7.5.1 cells, and JFH1-infected Huh7.5.1 cells (analyzed at 6 and 12 h and 1, 2, 3, 5, and 7 days postinfection) were cultured and lysed

    Journal: Journal of Virology

    Article Title: Suppressor of Cytokine Signaling 3 Suppresses Hepatitis C Virus Replication in an mTOR-Dependent Manner ▿

    doi: 10.1128/JVI.02484-09

    Figure Lengend Snippet: HCV replication decreases SOCS3 protein expression. Huh7 cells, OR6 cells cured of the HCV replicon, OR6 cells, Huh7.5.1 cells, and JFH1-infected Huh7.5.1 cells (analyzed at 6 and 12 h and 1, 2, 3, 5, and 7 days postinfection) were cultured and lysed

    Article Snippet: Protein A/G beads were incubated with mouse anti-SOCS3 (Abcam, Inc., Cambridge, MA) at 4°C with rolling for 2 h. After incubation, the beads were washed three times with phosphate-buffered saline (PBS).

    Techniques: Expressing, Infection, Cell Culture

    HCV individual protein constructs and transfection with a replication-defective viral RNA do not alter SOCS3 expression. (A) Transfection of Huh7.5.1 cells with the empty vectors pCAG and pCMV or pCMV-driven vectors expressing full-length HCV core protein

    Journal: Journal of Virology

    Article Title: Suppressor of Cytokine Signaling 3 Suppresses Hepatitis C Virus Replication in an mTOR-Dependent Manner ▿

    doi: 10.1128/JVI.02484-09

    Figure Lengend Snippet: HCV individual protein constructs and transfection with a replication-defective viral RNA do not alter SOCS3 expression. (A) Transfection of Huh7.5.1 cells with the empty vectors pCAG and pCMV or pCMV-driven vectors expressing full-length HCV core protein

    Article Snippet: Protein A/G beads were incubated with mouse anti-SOCS3 (Abcam, Inc., Cambridge, MA) at 4°C with rolling for 2 h. After incubation, the beads were washed three times with phosphate-buffered saline (PBS).

    Techniques: Construct, Transfection, Expressing

    SOCS3 downregulates HCV replication in replicon-carrying OR6 cells. OR6 cells were transfected with the pCDNA3-Myc or pCDNA3-Myc-hSOCS3 plasmid, and 72 h later the cells were lysed for Western blotting, Renilla luciferase assay, and RT-qPCR analyses as

    Journal: Journal of Virology

    Article Title: Suppressor of Cytokine Signaling 3 Suppresses Hepatitis C Virus Replication in an mTOR-Dependent Manner ▿

    doi: 10.1128/JVI.02484-09

    Figure Lengend Snippet: SOCS3 downregulates HCV replication in replicon-carrying OR6 cells. OR6 cells were transfected with the pCDNA3-Myc or pCDNA3-Myc-hSOCS3 plasmid, and 72 h later the cells were lysed for Western blotting, Renilla luciferase assay, and RT-qPCR analyses as

    Article Snippet: Protein A/G beads were incubated with mouse anti-SOCS3 (Abcam, Inc., Cambridge, MA) at 4°C with rolling for 2 h. After incubation, the beads were washed three times with phosphate-buffered saline (PBS).

    Techniques: Transfection, Plasmid Preparation, Western Blot, Luciferase, Quantitative RT-PCR

    mTOR inhibition reverses the inhibitory effects of SOCS3 on HCV replication in replicon-harboring OR6 cells. The replicon-carrying OR6 cells and cured OR6 cells were transfected with the pCDNA3-Myc or pCDNA3-Myc-hSOCS3 plasmid for 24 h and then treated

    Journal: Journal of Virology

    Article Title: Suppressor of Cytokine Signaling 3 Suppresses Hepatitis C Virus Replication in an mTOR-Dependent Manner ▿

    doi: 10.1128/JVI.02484-09

    Figure Lengend Snippet: mTOR inhibition reverses the inhibitory effects of SOCS3 on HCV replication in replicon-harboring OR6 cells. The replicon-carrying OR6 cells and cured OR6 cells were transfected with the pCDNA3-Myc or pCDNA3-Myc-hSOCS3 plasmid for 24 h and then treated

    Article Snippet: Protein A/G beads were incubated with mouse anti-SOCS3 (Abcam, Inc., Cambridge, MA) at 4°C with rolling for 2 h. After incubation, the beads were washed three times with phosphate-buffered saline (PBS).

    Techniques: Inhibition, Transfection, Plasmid Preparation

    Loss of SOCS3 in CD4 + T cells promotes the expansion of regulatory T cells and their suppressive activities. (A) Naïve CD4 + T cells were isolated and purified from lymph nodes and spleen of WT or CD4-SOCS3KO mice and stimulated with anti-CD3/CD28

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: SOCS3 Deletion in T-Lymphocytes Suppresses Development of Chronic Ocular Inflammation Via Up-regulation of CTLA-4 and Expansion of Regulatory T cells

    doi: 10.4049/jimmunol.1301132

    Figure Lengend Snippet: Loss of SOCS3 in CD4 + T cells promotes the expansion of regulatory T cells and their suppressive activities. (A) Naïve CD4 + T cells were isolated and purified from lymph nodes and spleen of WT or CD4-SOCS3KO mice and stimulated with anti-CD3/CD28

    Article Snippet: We isolated naïve CD4+ T cells from the WT or CD4-SOCS3KO mice, purified the cells using anti-CD4 magnetic beads, stimulated them for 3 days with anti-CD3/CD28 in the presence of TGF-β1 and examined whether SOCS3 regulates the expansion of Foxp3+ Tregs.

    Techniques: Isolation, Purification, Mouse Assay

    SOCS3-deficient CD4 + T cells promote the expansion of IL-10-producing cells. Naïve CD4 + T cells isolated from WT and CD4-SOCS3KO mice were stimulated with anti-CD3/CD28 for four days under Th0 or Th17 polarizing condition and then analyzed for

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: SOCS3 Deletion in T-Lymphocytes Suppresses Development of Chronic Ocular Inflammation Via Up-regulation of CTLA-4 and Expansion of Regulatory T cells

    doi: 10.4049/jimmunol.1301132

    Figure Lengend Snippet: SOCS3-deficient CD4 + T cells promote the expansion of IL-10-producing cells. Naïve CD4 + T cells isolated from WT and CD4-SOCS3KO mice were stimulated with anti-CD3/CD28 for four days under Th0 or Th17 polarizing condition and then analyzed for

    Article Snippet: We isolated naïve CD4+ T cells from the WT or CD4-SOCS3KO mice, purified the cells using anti-CD4 magnetic beads, stimulated them for 3 days with anti-CD3/CD28 in the presence of TGF-β1 and examined whether SOCS3 regulates the expansion of Foxp3+ Tregs.

    Techniques: Isolation, Mouse Assay

    Loss of SOCS3 in CD4 + T cells confers protection from uveitis. EAU was induced in WT or CD4-SOCS3KO mice by active immunization with IRBP in CFA. Progression and severity of EAU was monitored for 90 days by funduscopy or histology. (A) Fundus images were

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: SOCS3 Deletion in T-Lymphocytes Suppresses Development of Chronic Ocular Inflammation Via Up-regulation of CTLA-4 and Expansion of Regulatory T cells

    doi: 10.4049/jimmunol.1301132

    Figure Lengend Snippet: Loss of SOCS3 in CD4 + T cells confers protection from uveitis. EAU was induced in WT or CD4-SOCS3KO mice by active immunization with IRBP in CFA. Progression and severity of EAU was monitored for 90 days by funduscopy or histology. (A) Fundus images were

    Article Snippet: We isolated naïve CD4+ T cells from the WT or CD4-SOCS3KO mice, purified the cells using anti-CD4 magnetic beads, stimulated them for 3 days with anti-CD3/CD28 in the presence of TGF-β1 and examined whether SOCS3 regulates the expansion of Foxp3+ Tregs.

    Techniques: Mouse Assay

    SOCS3 promotes an anti-inflammatory response in vivo . (A) Freshly isolated PBMCs of un-immunized mice or IRBP-immunized mice were analyzed for expression of T cell cell-surface proteins by FACS. (B–D) The CD4 + T cells were also analyzed by the

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: SOCS3 Deletion in T-Lymphocytes Suppresses Development of Chronic Ocular Inflammation Via Up-regulation of CTLA-4 and Expansion of Regulatory T cells

    doi: 10.4049/jimmunol.1301132

    Figure Lengend Snippet: SOCS3 promotes an anti-inflammatory response in vivo . (A) Freshly isolated PBMCs of un-immunized mice or IRBP-immunized mice were analyzed for expression of T cell cell-surface proteins by FACS. (B–D) The CD4 + T cells were also analyzed by the

    Article Snippet: We isolated naïve CD4+ T cells from the WT or CD4-SOCS3KO mice, purified the cells using anti-CD4 magnetic beads, stimulated them for 3 days with anti-CD3/CD28 in the presence of TGF-β1 and examined whether SOCS3 regulates the expansion of Foxp3+ Tregs.

    Techniques: In Vivo, Isolation, Mouse Assay, Expressing, FACS

    SOCS3 interacts with CTLA-4 and down-regulates its expression in CD4 + T cells. (A) Naïve CD4 + T cells were stimulated by anti-CD3/CD28 for three days and analyzed by Western blotting. (B) Freshly isolated CD4 + T cells isolated from IRBP-immunized

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: SOCS3 Deletion in T-Lymphocytes Suppresses Development of Chronic Ocular Inflammation Via Up-regulation of CTLA-4 and Expansion of Regulatory T cells

    doi: 10.4049/jimmunol.1301132

    Figure Lengend Snippet: SOCS3 interacts with CTLA-4 and down-regulates its expression in CD4 + T cells. (A) Naïve CD4 + T cells were stimulated by anti-CD3/CD28 for three days and analyzed by Western blotting. (B) Freshly isolated CD4 + T cells isolated from IRBP-immunized

    Article Snippet: We isolated naïve CD4+ T cells from the WT or CD4-SOCS3KO mice, purified the cells using anti-CD4 magnetic beads, stimulated them for 3 days with anti-CD3/CD28 in the presence of TGF-β1 and examined whether SOCS3 regulates the expansion of Foxp3+ Tregs.

    Techniques: Expressing, Western Blot, Isolation

    Generstion and characterization of CD4-SOCS3KO mice. (A) SOCS3 fl/fl mice were cross-bred with CD4-Cre mice to generate mice with deletion of SOCS3 in the CD4 + T cells (CD4-SOCS3KO) and the SOCS3 fl/fl /Cre alleles were identified by PCR analysis of mouse

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: SOCS3 Deletion in T-Lymphocytes Suppresses Development of Chronic Ocular Inflammation Via Up-regulation of CTLA-4 and Expansion of Regulatory T cells

    doi: 10.4049/jimmunol.1301132

    Figure Lengend Snippet: Generstion and characterization of CD4-SOCS3KO mice. (A) SOCS3 fl/fl mice were cross-bred with CD4-Cre mice to generate mice with deletion of SOCS3 in the CD4 + T cells (CD4-SOCS3KO) and the SOCS3 fl/fl /Cre alleles were identified by PCR analysis of mouse

    Article Snippet: We isolated naïve CD4+ T cells from the WT or CD4-SOCS3KO mice, purified the cells using anti-CD4 magnetic beads, stimulated them for 3 days with anti-CD3/CD28 in the presence of TGF-β1 and examined whether SOCS3 regulates the expansion of Foxp3+ Tregs.

    Techniques: Mouse Assay, Polymerase Chain Reaction

    Antibody responces in vaccinated mice. A shows the IgG antibody responces to the carrier protein AcrA in vaccinated mice, measured using a luminex assay. B shows the IgG antibody responces to type 4 polysaccharide in vaccinated mice, measured using a Luminex assay. Antibody responces were measured in sera taken pre challenge with TIGR4. Each dot represent antibody levels in a single animal. Red circular symbol indicates the antibody levels in the mouse that was vaccinated with AcrA-SP4 P2 and survived challenge. Statistical analysis was performed in GraphPad Prism using a Kruskal-Wallis one way ANOVA with a Dunn’s multiple comparison test, *P

    Journal: Vaccine

    Article Title: Production and efficacy of a low-cost recombinant pneumococcal protein polysaccharide conjugate vaccine

    doi: 10.1016/j.vaccine.2018.05.036

    Figure Lengend Snippet: Antibody responces in vaccinated mice. A shows the IgG antibody responces to the carrier protein AcrA in vaccinated mice, measured using a luminex assay. B shows the IgG antibody responces to type 4 polysaccharide in vaccinated mice, measured using a Luminex assay. Antibody responces were measured in sera taken pre challenge with TIGR4. Each dot represent antibody levels in a single animal. Red circular symbol indicates the antibody levels in the mouse that was vaccinated with AcrA-SP4 P2 and survived challenge. Statistical analysis was performed in GraphPad Prism using a Kruskal-Wallis one way ANOVA with a Dunn’s multiple comparison test, *P

    Article Snippet: 2.7 Preparation of Luminex beads Protein (AcrA) and polysaccharide (type 4 polysaccharide) were coupled to carboxylate microspheres specific for use in the Luminex multiplex machine as described previously , .

    Techniques: Mouse Assay, Luminex

    Antibody responces in vaccinated mice. A shows the IgG antibody responces to the carrier protein AcrA in vaccinated mice, measured using a luminex assay. B shows the IgG antibody responces to type 4 polysaccharide in vaccinated mice, measured using a Luminex assay. Antibody responces were measured in sera taken pre challenge with TIGR4. Each dot represent antibody levels in a single animal. Red circular symbol indicates the antibody levels in the mouse that was vaccinated with AcrA-SP4 P2 and survived challenge. Statistical analysis was performed in GraphPad Prism using a Kruskal-Wallis one way ANOVA with a Dunn’s multiple comparison test, *P

    Journal: Vaccine

    Article Title: Production and efficacy of a low-cost recombinant pneumococcal protein polysaccharide conjugate vaccine

    doi: 10.1016/j.vaccine.2018.05.036

    Figure Lengend Snippet: Antibody responces in vaccinated mice. A shows the IgG antibody responces to the carrier protein AcrA in vaccinated mice, measured using a luminex assay. B shows the IgG antibody responces to type 4 polysaccharide in vaccinated mice, measured using a Luminex assay. Antibody responces were measured in sera taken pre challenge with TIGR4. Each dot represent antibody levels in a single animal. Red circular symbol indicates the antibody levels in the mouse that was vaccinated with AcrA-SP4 P2 and survived challenge. Statistical analysis was performed in GraphPad Prism using a Kruskal-Wallis one way ANOVA with a Dunn’s multiple comparison test, *P

    Article Snippet: 2.7 Preparation of Luminex beads Protein (AcrA) and polysaccharide (type 4 polysaccharide) were coupled to carboxylate microspheres specific for use in the Luminex multiplex machine as described previously , .

    Techniques: Mouse Assay, Luminex

    CD37 controls the IL-6 pathway via SOCS3, and lymphoma development is dependent on IL-6 in vivo.

    Journal: The Journal of Clinical Investigation

    Article Title: Tetraspanin CD37 protects against the development of B cell lymphoma

    doi: 10.1172/JCI81041

    Figure Lengend Snippet: CD37 controls the IL-6 pathway via SOCS3, and lymphoma development is dependent on IL-6 in vivo.

    Article Snippet: Samples were separated by SDS-PAGE, and Western blots were incubated with rabbit anti-GFP (made in-house) or rabbit anti-SOCS3 (Cell Signaling Technology, 2923) in TBS supplemented with 0.3% BSA and 0.1% skim milk powder at 4°C overnight and stained with secondary IRDye anti-rabbit antibodies (Invitrogen), followed by scanning membranes on the Odyssey Scanner (Westburg).

    Techniques: In Vivo

    PDGF-mediated SOCS3 induction in human corneal fibroblasts involves JAK2-STAT3 pathway. Human corneal fibroblasts were exposed to a mixture of PDGF (AA, AB, and BB) at a final concentration of 20 ng/ml with or without JAK2-STAT3 inhibitor, AG-490 (final concentration of 100 μM) for 1 h or 8 h. SOCS3 transcript levels were quantified by real-time PCR, and protein levels were determined by immunoprecipitation and immunoblotting using the SOCS3 polyclonal antibody. A : The fold change in SOCS3 transcript levels compared to untreated control is shown as mean ±SEM. The PDGF treatment increases SOCS3 mRNA levels by threefold at 1 h and 4.5 fold at 8 h, and AG-490 treatment inhibits PDGF-induced SOCS3 mRNA levels significantly. The asterisk indicates a p

    Journal: Molecular Vision

    Article Title: PDGF-driven proliferation, migration, and IL8 chemokine secretion in human corneal fibroblasts involve JAK2-STAT3 signaling pathway

    doi:

    Figure Lengend Snippet: PDGF-mediated SOCS3 induction in human corneal fibroblasts involves JAK2-STAT3 pathway. Human corneal fibroblasts were exposed to a mixture of PDGF (AA, AB, and BB) at a final concentration of 20 ng/ml with or without JAK2-STAT3 inhibitor, AG-490 (final concentration of 100 μM) for 1 h or 8 h. SOCS3 transcript levels were quantified by real-time PCR, and protein levels were determined by immunoprecipitation and immunoblotting using the SOCS3 polyclonal antibody. A : The fold change in SOCS3 transcript levels compared to untreated control is shown as mean ±SEM. The PDGF treatment increases SOCS3 mRNA levels by threefold at 1 h and 4.5 fold at 8 h, and AG-490 treatment inhibits PDGF-induced SOCS3 mRNA levels significantly. The asterisk indicates a p

    Article Snippet: After preclearing, 5 µl of 1 mg/ml SOCS3 goat polyclonal antibody (Santa Cruz Biotechnology Inc.) was added to each treatment group and 5 µl of pre-immune serum served as the negative control [ ].

    Techniques: Concentration Assay, Real-time Polymerase Chain Reaction, Immunoprecipitation

    A , co-immunoprecipiation of endogenous SOCS3 and Brk. T47D breast cancer cell lysates were immunoprecipitated ( IP ) with control rabbit immunoglobulin ( cIg ) or rabbit anti-Brk. Western blots ( WB ) were performed with murine antibody to SOCS3 or Brk. Molecular

    Journal: The Journal of Biological Chemistry

    Article Title: Suppressor of Cytokine Signaling 3 Inhibits Breast Tumor Kinase Activation of STAT3 *

    doi: 10.1074/jbc.M111.334144

    Figure Lengend Snippet: A , co-immunoprecipiation of endogenous SOCS3 and Brk. T47D breast cancer cell lysates were immunoprecipitated ( IP ) with control rabbit immunoglobulin ( cIg ) or rabbit anti-Brk. Western blots ( WB ) were performed with murine antibody to SOCS3 or Brk. Molecular

    Article Snippet: Immunocomplexes were collected on protein G agarose (Invitrogen), separated by SDS-PAGE and analyzed by Western blot with murine anti-SOCS3 antibody (Santa Cruz Biotechnology SC-51669) and murine anti-Brk (Santa Cruz Biotechnology SC-66003).

    Techniques: Immunoprecipitation, Western Blot

    SOCS3 SH2 domain binds to Brk. A , COS1 cells were co-transfected with GFP-Brk and Flag-SOCS3 full-length ( FL ) or SOCS3 deletion mutants expressing 46–185 a.a, 130–225 a.a., or 46–129 a.a.. Cells lysates were immunoprecipitated

    Journal: The Journal of Biological Chemistry

    Article Title: Suppressor of Cytokine Signaling 3 Inhibits Breast Tumor Kinase Activation of STAT3 *

    doi: 10.1074/jbc.M111.334144

    Figure Lengend Snippet: SOCS3 SH2 domain binds to Brk. A , COS1 cells were co-transfected with GFP-Brk and Flag-SOCS3 full-length ( FL ) or SOCS3 deletion mutants expressing 46–185 a.a, 130–225 a.a., or 46–129 a.a.. Cells lysates were immunoprecipitated

    Article Snippet: Immunocomplexes were collected on protein G agarose (Invitrogen), separated by SDS-PAGE and analyzed by Western blot with murine anti-SOCS3 antibody (Santa Cruz Biotechnology SC-51669) and murine anti-Brk (Santa Cruz Biotechnology SC-66003).

    Techniques: Transfection, Expressing, Immunoprecipitation

    The tyrosine kinase domain in Brk mediates interaction with SOCS3. A , binding assays of SOCS3 and Brk deletion mutants. Bacterially expressed GST or GST-SOCS3 proteins were immobilized on glutathione-agarose beads and incubated with lysates from cells

    Journal: The Journal of Biological Chemistry

    Article Title: Suppressor of Cytokine Signaling 3 Inhibits Breast Tumor Kinase Activation of STAT3 *

    doi: 10.1074/jbc.M111.334144

    Figure Lengend Snippet: The tyrosine kinase domain in Brk mediates interaction with SOCS3. A , binding assays of SOCS3 and Brk deletion mutants. Bacterially expressed GST or GST-SOCS3 proteins were immobilized on glutathione-agarose beads and incubated with lysates from cells

    Article Snippet: Immunocomplexes were collected on protein G agarose (Invitrogen), separated by SDS-PAGE and analyzed by Western blot with murine anti-SOCS3 antibody (Santa Cruz Biotechnology SC-51669) and murine anti-Brk (Santa Cruz Biotechnology SC-66003).

    Techniques: Binding Assay, Incubation

    Requirement of SOCS3 KIR domain for Brk inhibition. A , linear diagram of SOCS3 deletion mutants. Numbers correspond to amino acids. B , COS1 cells were co-transfected with STAT3-GFP, Brk-V5 with or without Flag-SOCS3 full-length ( FL ) or SOCS3 deletion

    Journal: The Journal of Biological Chemistry

    Article Title: Suppressor of Cytokine Signaling 3 Inhibits Breast Tumor Kinase Activation of STAT3 *

    doi: 10.1074/jbc.M111.334144

    Figure Lengend Snippet: Requirement of SOCS3 KIR domain for Brk inhibition. A , linear diagram of SOCS3 deletion mutants. Numbers correspond to amino acids. B , COS1 cells were co-transfected with STAT3-GFP, Brk-V5 with or without Flag-SOCS3 full-length ( FL ) or SOCS3 deletion

    Article Snippet: Immunocomplexes were collected on protein G agarose (Invitrogen), separated by SDS-PAGE and analyzed by Western blot with murine anti-SOCS3 antibody (Santa Cruz Biotechnology SC-51669) and murine anti-Brk (Santa Cruz Biotechnology SC-66003).

    Techniques: Inhibition, Transfection

    SOCS3 inhibits growth of breast cancer cell line MDA-MB-231. A , lentivirus infection was used to stably express SOCS3 or GFP in the MDA-MB-231 cell line. Similar cell numbers were seeded and proliferation was measured by trypan blue exclusion. Results

    Journal: The Journal of Biological Chemistry

    Article Title: Suppressor of Cytokine Signaling 3 Inhibits Breast Tumor Kinase Activation of STAT3 *

    doi: 10.1074/jbc.M111.334144

    Figure Lengend Snippet: SOCS3 inhibits growth of breast cancer cell line MDA-MB-231. A , lentivirus infection was used to stably express SOCS3 or GFP in the MDA-MB-231 cell line. Similar cell numbers were seeded and proliferation was measured by trypan blue exclusion. Results

    Article Snippet: Immunocomplexes were collected on protein G agarose (Invitrogen), separated by SDS-PAGE and analyzed by Western blot with murine anti-SOCS3 antibody (Santa Cruz Biotechnology SC-51669) and murine anti-Brk (Santa Cruz Biotechnology SC-66003).

    Techniques: Multiple Displacement Amplification, Infection, Stable Transfection

    SOCS3 targets Brk for proteasomal degradation via the SOCS box. A , Brk levels decrease with SOCS3. Cells were transfected with tetracycline-inducible Brk, tetracycline repressor and His-Ub with or without Flag-SOCS3 for 24 h and treated with doxycycline

    Journal: The Journal of Biological Chemistry

    Article Title: Suppressor of Cytokine Signaling 3 Inhibits Breast Tumor Kinase Activation of STAT3 *

    doi: 10.1074/jbc.M111.334144

    Figure Lengend Snippet: SOCS3 targets Brk for proteasomal degradation via the SOCS box. A , Brk levels decrease with SOCS3. Cells were transfected with tetracycline-inducible Brk, tetracycline repressor and His-Ub with or without Flag-SOCS3 for 24 h and treated with doxycycline

    Article Snippet: Immunocomplexes were collected on protein G agarose (Invitrogen), separated by SDS-PAGE and analyzed by Western blot with murine anti-SOCS3 antibody (Santa Cruz Biotechnology SC-51669) and murine anti-Brk (Santa Cruz Biotechnology SC-66003).

    Techniques: Transfection

    The interaction between SOCS3 and Brk. A , co-immunoprecipitation between SOCS3 and Brk. COS1 cells were co-transfected with GFP-Brk and Flag-SOCS3 or empty vector. Forty-eight hours after transfection, the cells were lysed, immunoprecipitated ( IP ) with

    Journal: The Journal of Biological Chemistry

    Article Title: Suppressor of Cytokine Signaling 3 Inhibits Breast Tumor Kinase Activation of STAT3 *

    doi: 10.1074/jbc.M111.334144

    Figure Lengend Snippet: The interaction between SOCS3 and Brk. A , co-immunoprecipitation between SOCS3 and Brk. COS1 cells were co-transfected with GFP-Brk and Flag-SOCS3 or empty vector. Forty-eight hours after transfection, the cells were lysed, immunoprecipitated ( IP ) with

    Article Snippet: Immunocomplexes were collected on protein G agarose (Invitrogen), separated by SDS-PAGE and analyzed by Western blot with murine anti-SOCS3 antibody (Santa Cruz Biotechnology SC-51669) and murine anti-Brk (Santa Cruz Biotechnology SC-66003).

    Techniques: Immunoprecipitation, Transfection, Plasmid Preparation