ssc Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98
    Millipore ssc buffer
    Ssc Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 472 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ssc buffer/product/Millipore
    Average 98 stars, based on 472 article reviews
    Price from $9.99 to $1999.99
    ssc buffer - by Bioz Stars, 2020-05
    98/100 stars
      Buy from Supplier

    86
    Thermo Fisher standard saline citrate ssc buffer
    Standard Saline Citrate Ssc Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/standard saline citrate ssc buffer/product/Thermo Fisher
    Average 86 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    standard saline citrate ssc buffer - by Bioz Stars, 2020-05
    86/100 stars
      Buy from Supplier

    89
    Boehringer Mannheim standard saline citrate ssc
    Standard Saline Citrate Ssc, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 89/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/standard saline citrate ssc/product/Boehringer Mannheim
    Average 89 stars, based on 54 article reviews
    Price from $9.99 to $1999.99
    standard saline citrate ssc - by Bioz Stars, 2020-05
    89/100 stars
      Buy from Supplier

    89
    Kodak Corp standard saline citrate ssc
    Standard Saline Citrate Ssc, supplied by Kodak Corp, used in various techniques. Bioz Stars score: 89/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/standard saline citrate ssc/product/Kodak Corp
    Average 89 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    standard saline citrate ssc - by Bioz Stars, 2020-05
    89/100 stars
      Buy from Supplier

    89
    Roche standard saline citrate ssc
    Standard Saline Citrate Ssc, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/standard saline citrate ssc/product/Roche
    Average 89 stars, based on 66 article reviews
    Price from $9.99 to $1999.99
    standard saline citrate ssc - by Bioz Stars, 2020-05
    89/100 stars
      Buy from Supplier

    89
    Agilent technologies standard saline citrate ssc
    Standard Saline Citrate Ssc, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/standard saline citrate ssc/product/Agilent technologies
    Average 89 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    standard saline citrate ssc - by Bioz Stars, 2020-05
    89/100 stars
      Buy from Supplier

    89
    GE Healthcare standard saline citrate ssc
    Standard Saline Citrate Ssc, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 89/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/standard saline citrate ssc/product/GE Healthcare
    Average 89 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    standard saline citrate ssc - by Bioz Stars, 2020-05
    89/100 stars
      Buy from Supplier

    89
    FUJIFILM standard saline citrate ssc
    Standard Saline Citrate Ssc, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 89/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/standard saline citrate ssc/product/FUJIFILM
    Average 89 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    standard saline citrate ssc - by Bioz Stars, 2020-05
    89/100 stars
      Buy from Supplier

    89
    Stratagene standard saline citrate ssc
    Standard Saline Citrate Ssc, supplied by Stratagene, used in various techniques. Bioz Stars score: 89/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/standard saline citrate ssc/product/Stratagene
    Average 89 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    standard saline citrate ssc - by Bioz Stars, 2020-05
    89/100 stars
      Buy from Supplier

    89
    Corning Life Sciences standard saline citrate ssc
    Standard Saline Citrate Ssc, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 89/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/standard saline citrate ssc/product/Corning Life Sciences
    Average 89 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    standard saline citrate ssc - by Bioz Stars, 2020-05
    89/100 stars
      Buy from Supplier

    80
    5 PRIME standard saline citrate ssc buffer
    Standard Saline Citrate Ssc Buffer, supplied by 5 PRIME, used in various techniques. Bioz Stars score: 80/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/standard saline citrate ssc buffer/product/5 PRIME
    Average 80 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    standard saline citrate ssc buffer - by Bioz Stars, 2020-05
    80/100 stars
      Buy from Supplier

    93
    Thermo Fisher ssc
    Distribution of log2 expression ratios. Labeled mouse liver RNA (Cy5) and Universal Mouse Reference RNA samples (Cy3) were hybridized with MWG mouse 20 K using <t>GlassHyb™</t> buffer or 5× <t>SSC,</t> 0.1% SDS buffer with different concentrations of formamide: A. GlassHyb™ buffer. B. 27% formamide. C. 35% formamide. D. 40% formamide. The log2 expression ratio (liver/reference) is plotted on the x-axis and the probability is on the y-axis.
    Ssc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1871 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ssc/product/Thermo Fisher
    Average 93 stars, based on 1871 article reviews
    Price from $9.99 to $1999.99
    ssc - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    92
    Millipore ssc
    Expansion of Th17 cells in <t>PBMCs</t> of patients with active <t>SSc.</t> (A) Human PBMCs were labeled with antibody against lymphocytes (anti-CD3 and -CD8). IL-17-expressing cells were detected by intracellular cytokine staining assay, and the percentage of IL-17 + cells among CD3 + CD8 - T cells was determined with flow cytometry. (B) Results of flow-cytometric analysis of Th17 cells in patients with active SSc ( n = 13), patients with stable SSc ( n = 32), and controls ( n = 24). (C) Longitudinal monitoring of Th17 cells in 10 patients. The percentage of Th17 cells was measured initially during active SSc and again after resolution after treatment. (D) Real-time RT-PCR analysis of IL-17, Foxp3, and RORγt mRNA expression in freshly isolated PBMCs of patients with active SSc ( n = 13), patients with stable SSc (n = 32), and controls (n = 24). (E) Positive correlation between the proportion of Th17 cells and clinical severity in active SSc patients, scored by using the Valentini score ( n = 13). r = 0.675, P
    Ssc, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 1575 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ssc/product/Millipore
    Average 92 stars, based on 1575 article reviews
    Price from $9.99 to $1999.99
    ssc - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    88
    Abbott Laboratories 2x standard saline citrate ssc buffer
    Expansion of Th17 cells in <t>PBMCs</t> of patients with active <t>SSc.</t> (A) Human PBMCs were labeled with antibody against lymphocytes (anti-CD3 and -CD8). IL-17-expressing cells were detected by intracellular cytokine staining assay, and the percentage of IL-17 + cells among CD3 + CD8 - T cells was determined with flow cytometry. (B) Results of flow-cytometric analysis of Th17 cells in patients with active SSc ( n = 13), patients with stable SSc ( n = 32), and controls ( n = 24). (C) Longitudinal monitoring of Th17 cells in 10 patients. The percentage of Th17 cells was measured initially during active SSc and again after resolution after treatment. (D) Real-time RT-PCR analysis of IL-17, Foxp3, and RORγt mRNA expression in freshly isolated PBMCs of patients with active SSc ( n = 13), patients with stable SSc (n = 32), and controls (n = 24). (E) Positive correlation between the proportion of Th17 cells and clinical severity in active SSc patients, scored by using the Valentini score ( n = 13). r = 0.675, P
    2x Standard Saline Citrate Ssc Buffer, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2x standard saline citrate ssc buffer/product/Abbott Laboratories
    Average 88 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    2x standard saline citrate ssc buffer - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

    85
    Telechem Corporation 3×standard saline citrate ssc
    Expansion of Th17 cells in <t>PBMCs</t> of patients with active <t>SSc.</t> (A) Human PBMCs were labeled with antibody against lymphocytes (anti-CD3 and -CD8). IL-17-expressing cells were detected by intracellular cytokine staining assay, and the percentage of IL-17 + cells among CD3 + CD8 - T cells was determined with flow cytometry. (B) Results of flow-cytometric analysis of Th17 cells in patients with active SSc ( n = 13), patients with stable SSc ( n = 32), and controls ( n = 24). (C) Longitudinal monitoring of Th17 cells in 10 patients. The percentage of Th17 cells was measured initially during active SSc and again after resolution after treatment. (D) Real-time RT-PCR analysis of IL-17, Foxp3, and RORγt mRNA expression in freshly isolated PBMCs of patients with active SSc ( n = 13), patients with stable SSc (n = 32), and controls (n = 24). (E) Positive correlation between the proportion of Th17 cells and clinical severity in active SSc patients, scored by using the Valentini score ( n = 13). r = 0.675, P
    3×Standard Saline Citrate Ssc, supplied by Telechem Corporation, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3×standard saline citrate ssc/product/Telechem Corporation
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    3×standard saline citrate ssc - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

    80
    Boehringer Mannheim 5×standard saline citrate ssc
    Expansion of Th17 cells in <t>PBMCs</t> of patients with active <t>SSc.</t> (A) Human PBMCs were labeled with antibody against lymphocytes (anti-CD3 and -CD8). IL-17-expressing cells were detected by intracellular cytokine staining assay, and the percentage of IL-17 + cells among CD3 + CD8 - T cells was determined with flow cytometry. (B) Results of flow-cytometric analysis of Th17 cells in patients with active SSc ( n = 13), patients with stable SSc ( n = 32), and controls ( n = 24). (C) Longitudinal monitoring of Th17 cells in 10 patients. The percentage of Th17 cells was measured initially during active SSc and again after resolution after treatment. (D) Real-time RT-PCR analysis of IL-17, Foxp3, and RORγt mRNA expression in freshly isolated PBMCs of patients with active SSc ( n = 13), patients with stable SSc (n = 32), and controls (n = 24). (E) Positive correlation between the proportion of Th17 cells and clinical severity in active SSc patients, scored by using the Valentini score ( n = 13). r = 0.675, P
    5×Standard Saline Citrate Ssc, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 80/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5×standard saline citrate ssc/product/Boehringer Mannheim
    Average 80 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    5×standard saline citrate ssc - by Bioz Stars, 2020-05
    80/100 stars
      Buy from Supplier

    91
    Thermo Fisher 20x standard saline citrate ssc buffer
    Expansion of Th17 cells in <t>PBMCs</t> of patients with active <t>SSc.</t> (A) Human PBMCs were labeled with antibody against lymphocytes (anti-CD3 and -CD8). IL-17-expressing cells were detected by intracellular cytokine staining assay, and the percentage of IL-17 + cells among CD3 + CD8 - T cells was determined with flow cytometry. (B) Results of flow-cytometric analysis of Th17 cells in patients with active SSc ( n = 13), patients with stable SSc ( n = 32), and controls ( n = 24). (C) Longitudinal monitoring of Th17 cells in 10 patients. The percentage of Th17 cells was measured initially during active SSc and again after resolution after treatment. (D) Real-time RT-PCR analysis of IL-17, Foxp3, and RORγt mRNA expression in freshly isolated PBMCs of patients with active SSc ( n = 13), patients with stable SSc (n = 32), and controls (n = 24). (E) Positive correlation between the proportion of Th17 cells and clinical severity in active SSc patients, scored by using the Valentini score ( n = 13). r = 0.675, P
    20x Standard Saline Citrate Ssc Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/20x standard saline citrate ssc buffer/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    20x standard saline citrate ssc buffer - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    92
    Millipore 2x ssc
    Expansion of Th17 cells in <t>PBMCs</t> of patients with active <t>SSc.</t> (A) Human PBMCs were labeled with antibody against lymphocytes (anti-CD3 and -CD8). IL-17-expressing cells were detected by intracellular cytokine staining assay, and the percentage of IL-17 + cells among CD3 + CD8 - T cells was determined with flow cytometry. (B) Results of flow-cytometric analysis of Th17 cells in patients with active SSc ( n = 13), patients with stable SSc ( n = 32), and controls ( n = 24). (C) Longitudinal monitoring of Th17 cells in 10 patients. The percentage of Th17 cells was measured initially during active SSc and again after resolution after treatment. (D) Real-time RT-PCR analysis of IL-17, Foxp3, and RORγt mRNA expression in freshly isolated PBMCs of patients with active SSc ( n = 13), patients with stable SSc (n = 32), and controls (n = 24). (E) Positive correlation between the proportion of Th17 cells and clinical severity in active SSc patients, scored by using the Valentini score ( n = 13). r = 0.675, P
    2x Ssc, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 294 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2x ssc/product/Millipore
    Average 92 stars, based on 294 article reviews
    Price from $9.99 to $1999.99
    2x ssc - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    91
    Boster Bio standard saline citrate
    Expansion of Th17 cells in <t>PBMCs</t> of patients with active <t>SSc.</t> (A) Human PBMCs were labeled with antibody against lymphocytes (anti-CD3 and -CD8). IL-17-expressing cells were detected by intracellular cytokine staining assay, and the percentage of IL-17 + cells among CD3 + CD8 - T cells was determined with flow cytometry. (B) Results of flow-cytometric analysis of Th17 cells in patients with active SSc ( n = 13), patients with stable SSc ( n = 32), and controls ( n = 24). (C) Longitudinal monitoring of Th17 cells in 10 patients. The percentage of Th17 cells was measured initially during active SSc and again after resolution after treatment. (D) Real-time RT-PCR analysis of IL-17, Foxp3, and RORγt mRNA expression in freshly isolated PBMCs of patients with active SSc ( n = 13), patients with stable SSc (n = 32), and controls (n = 24). (E) Positive correlation between the proportion of Th17 cells and clinical severity in active SSc patients, scored by using the Valentini score ( n = 13). r = 0.675, P
    Standard Saline Citrate, supplied by Boster Bio, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/standard saline citrate/product/Boster Bio
    Average 91 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    standard saline citrate - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    92
    Merck & Co ssc
    Expansion of Th17 cells in <t>PBMCs</t> of patients with active <t>SSc.</t> (A) Human PBMCs were labeled with antibody against lymphocytes (anti-CD3 and -CD8). IL-17-expressing cells were detected by intracellular cytokine staining assay, and the percentage of IL-17 + cells among CD3 + CD8 - T cells was determined with flow cytometry. (B) Results of flow-cytometric analysis of Th17 cells in patients with active SSc ( n = 13), patients with stable SSc ( n = 32), and controls ( n = 24). (C) Longitudinal monitoring of Th17 cells in 10 patients. The percentage of Th17 cells was measured initially during active SSc and again after resolution after treatment. (D) Real-time RT-PCR analysis of IL-17, Foxp3, and RORγt mRNA expression in freshly isolated PBMCs of patients with active SSc ( n = 13), patients with stable SSc (n = 32), and controls (n = 24). (E) Positive correlation between the proportion of Th17 cells and clinical severity in active SSc patients, scored by using the Valentini score ( n = 13). r = 0.675, P
    Ssc, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ssc/product/Merck & Co
    Average 92 stars, based on 63 article reviews
    Price from $9.99 to $1999.99
    ssc - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    ssc  (Nacalai)
    90
    Nacalai ssc
    Expansion of Th17 cells in <t>PBMCs</t> of patients with active <t>SSc.</t> (A) Human PBMCs were labeled with antibody against lymphocytes (anti-CD3 and -CD8). IL-17-expressing cells were detected by intracellular cytokine staining assay, and the percentage of IL-17 + cells among CD3 + CD8 - T cells was determined with flow cytometry. (B) Results of flow-cytometric analysis of Th17 cells in patients with active SSc ( n = 13), patients with stable SSc ( n = 32), and controls ( n = 24). (C) Longitudinal monitoring of Th17 cells in 10 patients. The percentage of Th17 cells was measured initially during active SSc and again after resolution after treatment. (D) Real-time RT-PCR analysis of IL-17, Foxp3, and RORγt mRNA expression in freshly isolated PBMCs of patients with active SSc ( n = 13), patients with stable SSc (n = 32), and controls (n = 24). (E) Positive correlation between the proportion of Th17 cells and clinical severity in active SSc patients, scored by using the Valentini score ( n = 13). r = 0.675, P
    Ssc, supplied by Nacalai, used in various techniques. Bioz Stars score: 90/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ssc/product/Nacalai
    Average 90 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    ssc - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    91
    Kodak Corp standard saline citrate buffer
    Expansion of Th17 cells in <t>PBMCs</t> of patients with active <t>SSc.</t> (A) Human PBMCs were labeled with antibody against lymphocytes (anti-CD3 and -CD8). IL-17-expressing cells were detected by intracellular cytokine staining assay, and the percentage of IL-17 + cells among CD3 + CD8 - T cells was determined with flow cytometry. (B) Results of flow-cytometric analysis of Th17 cells in patients with active SSc ( n = 13), patients with stable SSc ( n = 32), and controls ( n = 24). (C) Longitudinal monitoring of Th17 cells in 10 patients. The percentage of Th17 cells was measured initially during active SSc and again after resolution after treatment. (D) Real-time RT-PCR analysis of IL-17, Foxp3, and RORγt mRNA expression in freshly isolated PBMCs of patients with active SSc ( n = 13), patients with stable SSc (n = 32), and controls (n = 24). (E) Positive correlation between the proportion of Th17 cells and clinical severity in active SSc patients, scored by using the Valentini score ( n = 13). r = 0.675, P
    Standard Saline Citrate Buffer, supplied by Kodak Corp, used in various techniques. Bioz Stars score: 91/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/standard saline citrate buffer/product/Kodak Corp
    Average 91 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    standard saline citrate buffer - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    Image Search Results


    Distribution of log2 expression ratios. Labeled mouse liver RNA (Cy5) and Universal Mouse Reference RNA samples (Cy3) were hybridized with MWG mouse 20 K using GlassHyb™ buffer or 5× SSC, 0.1% SDS buffer with different concentrations of formamide: A. GlassHyb™ buffer. B. 27% formamide. C. 35% formamide. D. 40% formamide. The log2 expression ratio (liver/reference) is plotted on the x-axis and the probability is on the y-axis.

    Journal: BMC Bioinformatics

    Article Title: Improvement in the Reproducibility and Accuracy of DNA Microarray Quantification by Optimizing Hybridization Conditions

    doi: 10.1186/1471-2105-7-S2-S17

    Figure Lengend Snippet: Distribution of log2 expression ratios. Labeled mouse liver RNA (Cy5) and Universal Mouse Reference RNA samples (Cy3) were hybridized with MWG mouse 20 K using GlassHyb™ buffer or 5× SSC, 0.1% SDS buffer with different concentrations of formamide: A. GlassHyb™ buffer. B. 27% formamide. C. 35% formamide. D. 40% formamide. The log2 expression ratio (liver/reference) is plotted on the x-axis and the probability is on the y-axis.

    Article Snippet: The samples were then mixed with 60 μl of pre-warmed hybridization buffer, either GlassHyb™ (Clontech) or 5× SSC (Ambion), 0.1% SDS (Sigma) with various formamide (Invitrogen) concentrations at 50°C.

    Techniques: Expressing, Labeling

    Signal-to-background ratios under different hybridization or washing conditions. A. GlassHyb™ buffer and washing condition 1. B. GlassHyb™ with more stringent washing condition 2. C. 5× SSC, 0.1% SDS and 32% formamide buffer with washing condition 1. Oligo, rat-specific probe; Lambda, bacteriophage lambda-specific probe; Blank, no probe printed. Replicate experiments were conducted and the correlation coefficients were 0.94, 0.97, and 0.97 for conditions shown in panels A, B, and C, respectively.

    Journal: BMC Bioinformatics

    Article Title: Improvement in the Reproducibility and Accuracy of DNA Microarray Quantification by Optimizing Hybridization Conditions

    doi: 10.1186/1471-2105-7-S2-S17

    Figure Lengend Snippet: Signal-to-background ratios under different hybridization or washing conditions. A. GlassHyb™ buffer and washing condition 1. B. GlassHyb™ with more stringent washing condition 2. C. 5× SSC, 0.1% SDS and 32% formamide buffer with washing condition 1. Oligo, rat-specific probe; Lambda, bacteriophage lambda-specific probe; Blank, no probe printed. Replicate experiments were conducted and the correlation coefficients were 0.94, 0.97, and 0.97 for conditions shown in panels A, B, and C, respectively.

    Article Snippet: The samples were then mixed with 60 μl of pre-warmed hybridization buffer, either GlassHyb™ (Clontech) or 5× SSC (Ambion), 0.1% SDS (Sigma) with various formamide (Invitrogen) concentrations at 50°C.

    Techniques: Hybridization

    Signal-to-background ratios under different hybridization conditions. Labeled mouse liver RNA (Cy5) and Universal Mouse Reference RNA samples (Cy3) were hybridized with MWG mouse 20 K using GlassHyb™ buffer or 5× SSC, 0.1% SDS buffer with different concentrations of formamide: A. GlassHyb™ buffer. B. 27% formamide. C. 35% formamide. D. 40% formamide. Oligo, mouse-specific probe; Control, Arabidopsis-specific probe; Blank, no probe printed.

    Journal: BMC Bioinformatics

    Article Title: Improvement in the Reproducibility and Accuracy of DNA Microarray Quantification by Optimizing Hybridization Conditions

    doi: 10.1186/1471-2105-7-S2-S17

    Figure Lengend Snippet: Signal-to-background ratios under different hybridization conditions. Labeled mouse liver RNA (Cy5) and Universal Mouse Reference RNA samples (Cy3) were hybridized with MWG mouse 20 K using GlassHyb™ buffer or 5× SSC, 0.1% SDS buffer with different concentrations of formamide: A. GlassHyb™ buffer. B. 27% formamide. C. 35% formamide. D. 40% formamide. Oligo, mouse-specific probe; Control, Arabidopsis-specific probe; Blank, no probe printed.

    Article Snippet: The samples were then mixed with 60 μl of pre-warmed hybridization buffer, either GlassHyb™ (Clontech) or 5× SSC (Ambion), 0.1% SDS (Sigma) with various formamide (Invitrogen) concentrations at 50°C.

    Techniques: Hybridization, Labeling

    Expansion of Th17 cells in PBMCs of patients with active SSc. (A) Human PBMCs were labeled with antibody against lymphocytes (anti-CD3 and -CD8). IL-17-expressing cells were detected by intracellular cytokine staining assay, and the percentage of IL-17 + cells among CD3 + CD8 - T cells was determined with flow cytometry. (B) Results of flow-cytometric analysis of Th17 cells in patients with active SSc ( n = 13), patients with stable SSc ( n = 32), and controls ( n = 24). (C) Longitudinal monitoring of Th17 cells in 10 patients. The percentage of Th17 cells was measured initially during active SSc and again after resolution after treatment. (D) Real-time RT-PCR analysis of IL-17, Foxp3, and RORγt mRNA expression in freshly isolated PBMCs of patients with active SSc ( n = 13), patients with stable SSc (n = 32), and controls (n = 24). (E) Positive correlation between the proportion of Th17 cells and clinical severity in active SSc patients, scored by using the Valentini score ( n = 13). r = 0.675, P

    Journal: Arthritis Research & Therapy

    Article Title: Increased frequency of Th17 cells in systemic sclerosis is related to disease activity and collagen overproduction

    doi: 10.1186/ar4430

    Figure Lengend Snippet: Expansion of Th17 cells in PBMCs of patients with active SSc. (A) Human PBMCs were labeled with antibody against lymphocytes (anti-CD3 and -CD8). IL-17-expressing cells were detected by intracellular cytokine staining assay, and the percentage of IL-17 + cells among CD3 + CD8 - T cells was determined with flow cytometry. (B) Results of flow-cytometric analysis of Th17 cells in patients with active SSc ( n = 13), patients with stable SSc ( n = 32), and controls ( n = 24). (C) Longitudinal monitoring of Th17 cells in 10 patients. The percentage of Th17 cells was measured initially during active SSc and again after resolution after treatment. (D) Real-time RT-PCR analysis of IL-17, Foxp3, and RORγt mRNA expression in freshly isolated PBMCs of patients with active SSc ( n = 13), patients with stable SSc (n = 32), and controls (n = 24). (E) Positive correlation between the proportion of Th17 cells and clinical severity in active SSc patients, scored by using the Valentini score ( n = 13). r = 0.675, P

    Article Snippet: To determine the effect of secreted IL-17 on collagen production, PBMCs from patients with active SSc were incubated for 4 to 5 hours with PI (Sigma-Aldrich), and supernatants were collected for later use.

    Techniques: Labeling, Expressing, Staining, Flow Cytometry, Cytometry, Quantitative RT-PCR, Isolation

    IL-17 derived from SSc patients induces collagen production in fibroblasts. (A) IL-17 concentration in sera of patients with active SSc ( n = 13), patients with stable SSc ( n = 32), and healthy controls ( n = 24). (B) PBMCs from patients with active SSc ( n = 6), patients with stable SSc ( n = 6), and healthy controls ( n = 6) were stimulated for 5 hours with PMA and ionomycin, and IL-17 in the supernatant was measured with ELISA. (C) Fibroblasts were stimulated for 48 hours with supernatants from cultures of PI-stimulated PBMCs from healthy controls (Control) or, supernatants from cultures of PI-stimulated PBMCs from patients with active SSc (Active), and neutralization of IL-17 (α-IL-17), and the gene expression of collagen 1 and collagen 3 was measured by real-time RT-PCR analysis. Results shown are representative of at least three independent experiments. (D) Fibroblasts were stimulated for 48 hours with supernatants from cultures of PI-stimulated PBMCs from healthy controls (Control) or, supernatants from cultures of PI-stimulated PBMCs from patients with active SSc (Active), and neutralization of IL-17 (α-IL-17), and the levels of collagen 1 and collagen 3 in the supernatants were determined with ELISA. Results shown are representative of at least three independent experiments.

    Journal: Arthritis Research & Therapy

    Article Title: Increased frequency of Th17 cells in systemic sclerosis is related to disease activity and collagen overproduction

    doi: 10.1186/ar4430

    Figure Lengend Snippet: IL-17 derived from SSc patients induces collagen production in fibroblasts. (A) IL-17 concentration in sera of patients with active SSc ( n = 13), patients with stable SSc ( n = 32), and healthy controls ( n = 24). (B) PBMCs from patients with active SSc ( n = 6), patients with stable SSc ( n = 6), and healthy controls ( n = 6) were stimulated for 5 hours with PMA and ionomycin, and IL-17 in the supernatant was measured with ELISA. (C) Fibroblasts were stimulated for 48 hours with supernatants from cultures of PI-stimulated PBMCs from healthy controls (Control) or, supernatants from cultures of PI-stimulated PBMCs from patients with active SSc (Active), and neutralization of IL-17 (α-IL-17), and the gene expression of collagen 1 and collagen 3 was measured by real-time RT-PCR analysis. Results shown are representative of at least three independent experiments. (D) Fibroblasts were stimulated for 48 hours with supernatants from cultures of PI-stimulated PBMCs from healthy controls (Control) or, supernatants from cultures of PI-stimulated PBMCs from patients with active SSc (Active), and neutralization of IL-17 (α-IL-17), and the levels of collagen 1 and collagen 3 in the supernatants were determined with ELISA. Results shown are representative of at least three independent experiments.

    Article Snippet: To determine the effect of secreted IL-17 on collagen production, PBMCs from patients with active SSc were incubated for 4 to 5 hours with PI (Sigma-Aldrich), and supernatants were collected for later use.

    Techniques: Derivative Assay, Concentration Assay, Enzyme-linked Immunosorbent Assay, Neutralization, Expressing, Quantitative RT-PCR