Journal: Frontiers in Physiology
Article Title: Spexin Acts as Novel Regulator for Bile Acid Synthesis
Figure Lengend Snippet: Spexin inhibited bile acid synthesis through GALR2 and GALR3 receptors. (A) Expression of spexin, GALR2 and GALR2 in hepatocytes characterized by confocal imaging. To investigate the role of GALR2/3 in spexin-regulated bile acid synthesis, the mice were i.p . injected with GALR2/3 antagonists 15 min before spexin treatment. One hour after drug treatment, serum and liver samples from each mouse were collected for further analysis. Control ( i.p . with saline); Spexin ( i.p . with spexin 300 μg/kg); M871 ( i.p . with M871 1000 μg/kg); Spexin+M871 ( i.p . with M871 15 mins before spexin treatment); SNAP37889 ( i.p . with SNAP37889 1 mg/kg); Spexin+SNAP37889 ( i.p . with SNAP37889 15 min before spexin treatment). Hepatic level of TBA (B) , hepatic expression of CYP7A1 (C) and serum concentration of C4 (D) were measured as described. Results are shown as Mean ± SEM ( n = 10/group). * p
Article Snippet: Primary and secondary antibodies used: rabbit anti-spexin (Bachem, T-4858, 1:500), rabbit anti-GALR2 (Aviva Systems Biology, OABF00517, 1:100), rabbit anti-GALR3 (Aviva Systems Biology, OAAF04899, 1:100), AlexaFluor 594-conjugated goat anti-rabbit IgG (Thermofisher Scientific, , 1:500).
Techniques: Expressing, Imaging, Mouse Assay, Injection, Concentration Assay