Journal: Antimicrobial Agents and Chemotherapy
Article Title: Metallo-?-Lactamases in Clinical Pseudomonas Isolates in Taiwan and Identification of VIM-3, a Novel Variant of the VIM-2 Enzyme
Figure Lengend Snippet: PFGE profiles of Spe I-digested genomic DNAs from 21 Pseudomonas isolates carrying IMP- or VIM-type MBL genes. (A) Lanes 1 to 9 and 11, VIM-3-producing P. aeruginosa isolates NTU-23/99, PS-1205/00, PB-59, NTU-34/99, NTU-39/00, PS-64/99, NTU-26/99, NTU-33/99, NTU-43/00, and NTU-79/00, respectively; lane 10, VIM-2-producing P. putida isolate PS-693/00; lane M, a bacteriophage lambda ladder. (B) Lanes 1, 4, 6, 9, and 10, IMP-1 producer isolates PB-207, NTU-92/99, PB-166, PB-101, and PB-24, respectively; lanes 2, 3, 5, 7, and 8, VIM-2 producers isolates PS-1153/00, NTU-91/99, NTU-93/99, PS-579/00, and PS-679/00, respectively; lane M, a bacteriophage lambda ladder.
Article Snippet: Genomic DNAs prepared by the procedure of Piggot et al. ( ) were digested overnight with 10 U of Spe I (New England Biolabs, Beverly, Mass.) and subjected to pulsed-field gel electrophoresis (PFGE) as described elsewhere ( , ).