spectramax m5 fluorimeter Search Results


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  • 93
    Molecular Devices LLC spectramax m5 fluorimeter
    ROS is required for GA-induced autophagy. ( A ) Intracellular ROS in HCT116 cells treated with 1‰ DMSO (Control), 1.0 µM GA (GA), or 1.0 µM GA in the presence NAC (10 mM) (GA+NAC) for 24 h were detected by staining cells with 2′,7′-dichlorofluorescein diacetate under blue (B), or blue and white excitation (B+A). The DCFH-DA signal was measured using a Molecular Devices <t>SPECTRAMAX</t> M5 fluorimeter. ( B ) HCT116 cells transfected with a pEGFP-LC3 plasmid with or without GA (1.0 µM) or/and NAC (10 mM) for 24 h. Cells were defined as positive if they had 5 or more GFP-LC3 dots in the cytoplasm. The percentage of the cells with GFP-LC3 dots and the average number of GFP-LC3 dots per cell were analyzed from at least 100 random fields. ** p
    Spectramax M5 Fluorimeter, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 93/100, based on 134 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spectramax m5 fluorimeter/product/Molecular Devices LLC
    Average 93 stars, based on 134 article reviews
    Price from $9.99 to $1999.99
    spectramax m5 fluorimeter - by Bioz Stars, 2020-04
    93/100 stars
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    86
    Molecular Devices LLC fluorimeter spectramax m5 multi mode microplate reader
    ROS is required for GA-induced autophagy. ( A ) Intracellular ROS in HCT116 cells treated with 1‰ DMSO (Control), 1.0 µM GA (GA), or 1.0 µM GA in the presence NAC (10 mM) (GA+NAC) for 24 h were detected by staining cells with 2′,7′-dichlorofluorescein diacetate under blue (B), or blue and white excitation (B+A). The DCFH-DA signal was measured using a Molecular Devices <t>SPECTRAMAX</t> M5 fluorimeter. ( B ) HCT116 cells transfected with a pEGFP-LC3 plasmid with or without GA (1.0 µM) or/and NAC (10 mM) for 24 h. Cells were defined as positive if they had 5 or more GFP-LC3 dots in the cytoplasm. The percentage of the cells with GFP-LC3 dots and the average number of GFP-LC3 dots per cell were analyzed from at least 100 random fields. ** p
    Fluorimeter Spectramax M5 Multi Mode Microplate Reader, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorimeter spectramax m5 multi mode microplate reader/product/Molecular Devices LLC
    Average 86 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    fluorimeter spectramax m5 multi mode microplate reader - by Bioz Stars, 2020-04
    86/100 stars
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    85
    Molecular Devices LLC spectral fluorimeter
    ROS is required for GA-induced autophagy. ( A ) Intracellular ROS in HCT116 cells treated with 1‰ DMSO (Control), 1.0 µM GA (GA), or 1.0 µM GA in the presence NAC (10 mM) (GA+NAC) for 24 h were detected by staining cells with 2′,7′-dichlorofluorescein diacetate under blue (B), or blue and white excitation (B+A). The DCFH-DA signal was measured using a Molecular Devices <t>SPECTRAMAX</t> M5 fluorimeter. ( B ) HCT116 cells transfected with a pEGFP-LC3 plasmid with or without GA (1.0 µM) or/and NAC (10 mM) for 24 h. Cells were defined as positive if they had 5 or more GFP-LC3 dots in the cytoplasm. The percentage of the cells with GFP-LC3 dots and the average number of GFP-LC3 dots per cell were analyzed from at least 100 random fields. ** p
    Spectral Fluorimeter, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spectral fluorimeter/product/Molecular Devices LLC
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    spectral fluorimeter - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

    95
    Molecular Devices LLC fluorimeter
    ROS is required for GA-induced autophagy. ( A ) Intracellular ROS in HCT116 cells treated with 1‰ DMSO (Control), 1.0 µM GA (GA), or 1.0 µM GA in the presence NAC (10 mM) (GA+NAC) for 24 h were detected by staining cells with 2′,7′-dichlorofluorescein diacetate under blue (B), or blue and white excitation (B+A). The DCFH-DA signal was measured using a Molecular Devices <t>SPECTRAMAX</t> M5 fluorimeter. ( B ) HCT116 cells transfected with a pEGFP-LC3 plasmid with or without GA (1.0 µM) or/and NAC (10 mM) for 24 h. Cells were defined as positive if they had 5 or more GFP-LC3 dots in the cytoplasm. The percentage of the cells with GFP-LC3 dots and the average number of GFP-LC3 dots per cell were analyzed from at least 100 random fields. ** p
    Fluorimeter, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 95/100, based on 190 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorimeter/product/Molecular Devices LLC
    Average 95 stars, based on 190 article reviews
    Price from $9.99 to $1999.99
    fluorimeter - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    94
    Molecular Devices LLC 96 well plate fluorimeter
    ROS is required for GA-induced autophagy. ( A ) Intracellular ROS in HCT116 cells treated with 1‰ DMSO (Control), 1.0 µM GA (GA), or 1.0 µM GA in the presence NAC (10 mM) (GA+NAC) for 24 h were detected by staining cells with 2′,7′-dichlorofluorescein diacetate under blue (B), or blue and white excitation (B+A). The DCFH-DA signal was measured using a Molecular Devices <t>SPECTRAMAX</t> M5 fluorimeter. ( B ) HCT116 cells transfected with a pEGFP-LC3 plasmid with or without GA (1.0 µM) or/and NAC (10 mM) for 24 h. Cells were defined as positive if they had 5 or more GFP-LC3 dots in the cytoplasm. The percentage of the cells with GFP-LC3 dots and the average number of GFP-LC3 dots per cell were analyzed from at least 100 random fields. ** p
    96 Well Plate Fluorimeter, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/96 well plate fluorimeter/product/Molecular Devices LLC
    Average 94 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    96 well plate fluorimeter - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    Image Search Results


    ROS is required for GA-induced autophagy. ( A ) Intracellular ROS in HCT116 cells treated with 1‰ DMSO (Control), 1.0 µM GA (GA), or 1.0 µM GA in the presence NAC (10 mM) (GA+NAC) for 24 h were detected by staining cells with 2′,7′-dichlorofluorescein diacetate under blue (B), or blue and white excitation (B+A). The DCFH-DA signal was measured using a Molecular Devices SPECTRAMAX M5 fluorimeter. ( B ) HCT116 cells transfected with a pEGFP-LC3 plasmid with or without GA (1.0 µM) or/and NAC (10 mM) for 24 h. Cells were defined as positive if they had 5 or more GFP-LC3 dots in the cytoplasm. The percentage of the cells with GFP-LC3 dots and the average number of GFP-LC3 dots per cell were analyzed from at least 100 random fields. ** p

    Journal: PLoS ONE

    Article Title: ROS-Mediated Autophagy Induced by Dysregulation of Lipid Metabolism Plays a Protective Role in Colorectal Cancer Cells Treated with Gambogic Acid

    doi: 10.1371/journal.pone.0096418

    Figure Lengend Snippet: ROS is required for GA-induced autophagy. ( A ) Intracellular ROS in HCT116 cells treated with 1‰ DMSO (Control), 1.0 µM GA (GA), or 1.0 µM GA in the presence NAC (10 mM) (GA+NAC) for 24 h were detected by staining cells with 2′,7′-dichlorofluorescein diacetate under blue (B), or blue and white excitation (B+A). The DCFH-DA signal was measured using a Molecular Devices SPECTRAMAX M5 fluorimeter. ( B ) HCT116 cells transfected with a pEGFP-LC3 plasmid with or without GA (1.0 µM) or/and NAC (10 mM) for 24 h. Cells were defined as positive if they had 5 or more GFP-LC3 dots in the cytoplasm. The percentage of the cells with GFP-LC3 dots and the average number of GFP-LC3 dots per cell were analyzed from at least 100 random fields. ** p

    Article Snippet: The DCFH-DA signal was measured with a Molecular Devices SPECTRAMAX M5 fluorimeter (490 nm excitation and 530 nm emission).

    Techniques: Staining, Transfection, Plasmid Preparation