Journal: Journal of Clinical Microbiology
Article Title: Rapid Identification of Candida dubliniensis Using a Species-Specific Molecular Beacon
Figure Lengend Snippet: The ITS2 region was PCR amplified with universal fungal primers ITS3 and ITS4, and the products were digested with restriction enzymes Bsm AI ( C. dubliniensis specific) (A) and Nsp BII ( C. albicans specific) (B). The restriction fragments were run on a 1.2% agarose gel and stained with GelStar (FMC Bioproducts). ∗, C. dubliniensis strains.
Article Snippet: The PCR amplification products (∼500 ng) were digested with 1 μl of restriction enzymes Nsp BII ( C. albicans ITS2 specific) (AP Biotech, Piscataway, N.J.) and Bsm AI ( C. dubliniensis ITS2 specific) (New England Biolabs, Beverly, Mass.) for 1 h and were analyzed by gel electrophoresis in a 1.5% agarose gel.
Techniques: Polymerase Chain Reaction, Amplification, Agarose Gel Electrophoresis, Staining