Journal: The Journal of Neuroscience
Article Title: Tonic Activation of GluN2C/GluN2D-Containing NMDA Receptors by Ambient Glutamate Facilitates Cortical Interneuron Maturation
Figure Lengend Snippet: G42 mice identify GABAergic non-SST INs at P9. a , Immunohistochemical labeling of GFP in the somatosensory cortex (SSC) of G42 (GAD1-eGFP) mice at P9. Coimmunolabeling of GFP in G42 mice with markers on inhibitory INs. a1Ã¢ÂÂa5 , GFP (left). a1 , GABA. a2 , Somatostatin. a3 , PV. a4 , GAD67. a5 , Calretinin (middle), colabel (right) in layer V of the SSC. b , Table showing abundant colabeling of GFP + cells with GABA immunolabeling at P9. Sections from 4 mice were used to quantify colocalization. c , Average evoked APs from whole-cell recordings from GFP + cells in G42 mice (red), L5Ps (black), and Td-tomato + neurons in SST-Cre X Ai9 mice (blue). d , dV/dt analysis of APs shown in c . e , AP half-width. f , AP threshold. g , Afterhyperpolarization amplitude. h , Fraction of cells showing spontaneous AP firing in GFP + cells in G42 mice (red), L5P neurons (black), and Td-Tomato + neurons in SST-Cre X Ai9 mice (blue). * p
Article Snippet: The 40 ÃÂ¼m brain slices were stained, as described above, with primary antibodies against GFP (1:1000, chicken, Abcam) and one of the following markers: GABA (1:5000, rabbit, Sigma-Aldrich), parvalbumin (PV, 1:2000, mouse, Swant), glutamate decarboxylase 67 (GAD67, 1:10,000, mouse, Millipore), somatostatin (SST, 1:100, rat, Millipore), and calretinin (CalR, 1:5000, mouse, Swant).
Techniques: Mouse Assay, Immunohistochemistry, Labeling, Immunolabeling