sodium palmitate Search Results


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  • 99
    Thermo Fisher bodipy fl c16
    Smurf1 regulates fatty acid uptake and lipid synthesis through PPARγ. (A) Western blots showing that knockdown of Smurf1 but not Smurf2 in Hep3B and AML12 cells increased PPARγ protein level. (B) qRT-PCR analyses showing that knockdown of Smurf1 but not Smurf2 increased Ppar γ mRNA level in AML12 cells. (C) qRT-PCR analyses showing that knockdown of Smurf1 in AML12 cells increased expression of Fabp1 , Cd36 , Acacb , and Apoc3 in a PPARγ-dependent manner. (D) Fatty acid uptake in AML12 cells as measured by 3 H-palmitate incorporation ( n = 3). (E) Lipid synthesis in AML12 cells as measured by incorporation of 3 H-acetate into lipid ( n = 3). (F) In vivo fatty acid uptake after intraperitoneal injection of <t>BODIPY-FL-C16.</t> The BODIPY-FL-C16 accumulation in the liver, epididymal WAT, and skeletal muscle was normalized to tissue weight ( n = 8 per group). (G) Lipogenesis in primary hepatocytes as measured by the incorporation of 3 H-acetate into lipid ( n = 6 per group). Data are presented as mean ± SD; statistical significance of difference is indicated as * p
    Bodipy Fl C16, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 327 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore sodium palmitate
    Smurf1 regulates fatty acid uptake and lipid synthesis through PPARγ. (A) Western blots showing that knockdown of Smurf1 but not Smurf2 in Hep3B and AML12 cells increased PPARγ protein level. (B) qRT-PCR analyses showing that knockdown of Smurf1 but not Smurf2 increased Ppar γ mRNA level in AML12 cells. (C) qRT-PCR analyses showing that knockdown of Smurf1 in AML12 cells increased expression of Fabp1 , Cd36 , Acacb , and Apoc3 in a PPARγ-dependent manner. (D) Fatty acid uptake in AML12 cells as measured by 3 H-palmitate incorporation ( n = 3). (E) Lipid synthesis in AML12 cells as measured by incorporation of 3 H-acetate into lipid ( n = 3). (F) In vivo fatty acid uptake after intraperitoneal injection of <t>BODIPY-FL-C16.</t> The BODIPY-FL-C16 accumulation in the liver, epididymal WAT, and skeletal muscle was normalized to tissue weight ( n = 8 per group). (G) Lipogenesis in primary hepatocytes as measured by the incorporation of 3 H-acetate into lipid ( n = 6 per group). Data are presented as mean ± SD; statistical significance of difference is indicated as * p
    Sodium Palmitate, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 751 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Cayman Chemical palmitic acid
    Smurf1 regulates fatty acid uptake and lipid synthesis through PPARγ. (A) Western blots showing that knockdown of Smurf1 but not Smurf2 in Hep3B and AML12 cells increased PPARγ protein level. (B) qRT-PCR analyses showing that knockdown of Smurf1 but not Smurf2 increased Ppar γ mRNA level in AML12 cells. (C) qRT-PCR analyses showing that knockdown of Smurf1 in AML12 cells increased expression of Fabp1 , Cd36 , Acacb , and Apoc3 in a PPARγ-dependent manner. (D) Fatty acid uptake in AML12 cells as measured by 3 H-palmitate incorporation ( n = 3). (E) Lipid synthesis in AML12 cells as measured by incorporation of 3 H-acetate into lipid ( n = 3). (F) In vivo fatty acid uptake after intraperitoneal injection of <t>BODIPY-FL-C16.</t> The BODIPY-FL-C16 accumulation in the liver, epididymal WAT, and skeletal muscle was normalized to tissue weight ( n = 8 per group). (G) Lipogenesis in primary hepatocytes as measured by the incorporation of 3 H-acetate into lipid ( n = 6 per group). Data are presented as mean ± SD; statistical significance of difference is indicated as * p
    Palmitic Acid, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 93/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    PerkinElmer palmitic acid 910 3hn
    Smurf1 regulates fatty acid uptake and lipid synthesis through PPARγ. (A) Western blots showing that knockdown of Smurf1 but not Smurf2 in Hep3B and AML12 cells increased PPARγ protein level. (B) qRT-PCR analyses showing that knockdown of Smurf1 but not Smurf2 increased Ppar γ mRNA level in AML12 cells. (C) qRT-PCR analyses showing that knockdown of Smurf1 in AML12 cells increased expression of Fabp1 , Cd36 , Acacb , and Apoc3 in a PPARγ-dependent manner. (D) Fatty acid uptake in AML12 cells as measured by 3 H-palmitate incorporation ( n = 3). (E) Lipid synthesis in AML12 cells as measured by incorporation of 3 H-acetate into lipid ( n = 3). (F) In vivo fatty acid uptake after intraperitoneal injection of <t>BODIPY-FL-C16.</t> The BODIPY-FL-C16 accumulation in the liver, epididymal WAT, and skeletal muscle was normalized to tissue weight ( n = 8 per group). (G) Lipogenesis in primary hepatocytes as measured by the incorporation of 3 H-acetate into lipid ( n = 6 per group). Data are presented as mean ± SD; statistical significance of difference is indicated as * p
    Palmitic Acid 910 3hn, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 94/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    FUJIFILM palmitic acid
    Smurf1 regulates fatty acid uptake and lipid synthesis through PPARγ. (A) Western blots showing that knockdown of Smurf1 but not Smurf2 in Hep3B and AML12 cells increased PPARγ protein level. (B) qRT-PCR analyses showing that knockdown of Smurf1 but not Smurf2 increased Ppar γ mRNA level in AML12 cells. (C) qRT-PCR analyses showing that knockdown of Smurf1 in AML12 cells increased expression of Fabp1 , Cd36 , Acacb , and Apoc3 in a PPARγ-dependent manner. (D) Fatty acid uptake in AML12 cells as measured by 3 H-palmitate incorporation ( n = 3). (E) Lipid synthesis in AML12 cells as measured by incorporation of 3 H-acetate into lipid ( n = 3). (F) In vivo fatty acid uptake after intraperitoneal injection of <t>BODIPY-FL-C16.</t> The BODIPY-FL-C16 accumulation in the liver, epididymal WAT, and skeletal muscle was normalized to tissue weight ( n = 8 per group). (G) Lipogenesis in primary hepatocytes as measured by the incorporation of 3 H-acetate into lipid ( n = 6 per group). Data are presented as mean ± SD; statistical significance of difference is indicated as * p
    Palmitic Acid, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 92/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    American Radiolabeled Chemicals Inc h palmitic acid
    Smurf1 regulates fatty acid uptake and lipid synthesis through PPARγ. (A) Western blots showing that knockdown of Smurf1 but not Smurf2 in Hep3B and AML12 cells increased PPARγ protein level. (B) qRT-PCR analyses showing that knockdown of Smurf1 but not Smurf2 increased Ppar γ mRNA level in AML12 cells. (C) qRT-PCR analyses showing that knockdown of Smurf1 in AML12 cells increased expression of Fabp1 , Cd36 , Acacb , and Apoc3 in a PPARγ-dependent manner. (D) Fatty acid uptake in AML12 cells as measured by 3 H-palmitate incorporation ( n = 3). (E) Lipid synthesis in AML12 cells as measured by incorporation of 3 H-acetate into lipid ( n = 3). (F) In vivo fatty acid uptake after intraperitoneal injection of <t>BODIPY-FL-C16.</t> The BODIPY-FL-C16 accumulation in the liver, epididymal WAT, and skeletal muscle was normalized to tissue weight ( n = 8 per group). (G) Lipogenesis in primary hepatocytes as measured by the incorporation of 3 H-acetate into lipid ( n = 6 per group). Data are presented as mean ± SD; statistical significance of difference is indicated as * p
    H Palmitic Acid, supplied by American Radiolabeled Chemicals Inc, used in various techniques. Bioz Stars score: 91/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Millipore c palmitic acid
    Smurf1 regulates fatty acid uptake and lipid synthesis through PPARγ. (A) Western blots showing that knockdown of Smurf1 but not Smurf2 in Hep3B and AML12 cells increased PPARγ protein level. (B) qRT-PCR analyses showing that knockdown of Smurf1 but not Smurf2 increased Ppar γ mRNA level in AML12 cells. (C) qRT-PCR analyses showing that knockdown of Smurf1 in AML12 cells increased expression of Fabp1 , Cd36 , Acacb , and Apoc3 in a PPARγ-dependent manner. (D) Fatty acid uptake in AML12 cells as measured by 3 H-palmitate incorporation ( n = 3). (E) Lipid synthesis in AML12 cells as measured by incorporation of 3 H-acetate into lipid ( n = 3). (F) In vivo fatty acid uptake after intraperitoneal injection of <t>BODIPY-FL-C16.</t> The BODIPY-FL-C16 accumulation in the liver, epididymal WAT, and skeletal muscle was normalized to tissue weight ( n = 8 per group). (G) Lipogenesis in primary hepatocytes as measured by the incorporation of 3 H-acetate into lipid ( n = 6 per group). Data are presented as mean ± SD; statistical significance of difference is indicated as * p
    C Palmitic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Nu-Chek Prep palmitic acid
    Smurf1 regulates fatty acid uptake and lipid synthesis through PPARγ. (A) Western blots showing that knockdown of Smurf1 but not Smurf2 in Hep3B and AML12 cells increased PPARγ protein level. (B) qRT-PCR analyses showing that knockdown of Smurf1 but not Smurf2 increased Ppar γ mRNA level in AML12 cells. (C) qRT-PCR analyses showing that knockdown of Smurf1 in AML12 cells increased expression of Fabp1 , Cd36 , Acacb , and Apoc3 in a PPARγ-dependent manner. (D) Fatty acid uptake in AML12 cells as measured by 3 H-palmitate incorporation ( n = 3). (E) Lipid synthesis in AML12 cells as measured by incorporation of 3 H-acetate into lipid ( n = 3). (F) In vivo fatty acid uptake after intraperitoneal injection of <t>BODIPY-FL-C16.</t> The BODIPY-FL-C16 accumulation in the liver, epididymal WAT, and skeletal muscle was normalized to tissue weight ( n = 8 per group). (G) Lipogenesis in primary hepatocytes as measured by the incorporation of 3 H-acetate into lipid ( n = 6 per group). Data are presented as mean ± SD; statistical significance of difference is indicated as * p
    Palmitic Acid, supplied by Nu-Chek Prep, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore palmitic acids
    Smurf1 regulates fatty acid uptake and lipid synthesis through PPARγ. (A) Western blots showing that knockdown of Smurf1 but not Smurf2 in Hep3B and AML12 cells increased PPARγ protein level. (B) qRT-PCR analyses showing that knockdown of Smurf1 but not Smurf2 increased Ppar γ mRNA level in AML12 cells. (C) qRT-PCR analyses showing that knockdown of Smurf1 in AML12 cells increased expression of Fabp1 , Cd36 , Acacb , and Apoc3 in a PPARγ-dependent manner. (D) Fatty acid uptake in AML12 cells as measured by 3 H-palmitate incorporation ( n = 3). (E) Lipid synthesis in AML12 cells as measured by incorporation of 3 H-acetate into lipid ( n = 3). (F) In vivo fatty acid uptake after intraperitoneal injection of <t>BODIPY-FL-C16.</t> The BODIPY-FL-C16 accumulation in the liver, epididymal WAT, and skeletal muscle was normalized to tissue weight ( n = 8 per group). (G) Lipogenesis in primary hepatocytes as measured by the incorporation of 3 H-acetate into lipid ( n = 6 per group). Data are presented as mean ± SD; statistical significance of difference is indicated as * p
    Palmitic Acids, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Avanti Polar palmitic acid
    Smurf1 regulates fatty acid uptake and lipid synthesis through PPARγ. (A) Western blots showing that knockdown of Smurf1 but not Smurf2 in Hep3B and AML12 cells increased PPARγ protein level. (B) qRT-PCR analyses showing that knockdown of Smurf1 but not Smurf2 increased Ppar γ mRNA level in AML12 cells. (C) qRT-PCR analyses showing that knockdown of Smurf1 in AML12 cells increased expression of Fabp1 , Cd36 , Acacb , and Apoc3 in a PPARγ-dependent manner. (D) Fatty acid uptake in AML12 cells as measured by 3 H-palmitate incorporation ( n = 3). (E) Lipid synthesis in AML12 cells as measured by incorporation of 3 H-acetate into lipid ( n = 3). (F) In vivo fatty acid uptake after intraperitoneal injection of <t>BODIPY-FL-C16.</t> The BODIPY-FL-C16 accumulation in the liver, epididymal WAT, and skeletal muscle was normalized to tissue weight ( n = 8 per group). (G) Lipogenesis in primary hepatocytes as measured by the incorporation of 3 H-acetate into lipid ( n = 6 per group). Data are presented as mean ± SD; statistical significance of difference is indicated as * p
    Palmitic Acid, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Moravek Biochemicals c palmitic acid
    Smurf1 regulates fatty acid uptake and lipid synthesis through PPARγ. (A) Western blots showing that knockdown of Smurf1 but not Smurf2 in Hep3B and AML12 cells increased PPARγ protein level. (B) qRT-PCR analyses showing that knockdown of Smurf1 but not Smurf2 increased Ppar γ mRNA level in AML12 cells. (C) qRT-PCR analyses showing that knockdown of Smurf1 in AML12 cells increased expression of Fabp1 , Cd36 , Acacb , and Apoc3 in a PPARγ-dependent manner. (D) Fatty acid uptake in AML12 cells as measured by 3 H-palmitate incorporation ( n = 3). (E) Lipid synthesis in AML12 cells as measured by incorporation of 3 H-acetate into lipid ( n = 3). (F) In vivo fatty acid uptake after intraperitoneal injection of <t>BODIPY-FL-C16.</t> The BODIPY-FL-C16 accumulation in the liver, epididymal WAT, and skeletal muscle was normalized to tissue weight ( n = 8 per group). (G) Lipogenesis in primary hepatocytes as measured by the incorporation of 3 H-acetate into lipid ( n = 6 per group). Data are presented as mean ± SD; statistical significance of difference is indicated as * p
    C Palmitic Acid, supplied by Moravek Biochemicals, used in various techniques. Bioz Stars score: 90/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Cambridge Isotope Laboratories palmitic acid
    Smurf1 regulates fatty acid uptake and lipid synthesis through PPARγ. (A) Western blots showing that knockdown of Smurf1 but not Smurf2 in Hep3B and AML12 cells increased PPARγ protein level. (B) qRT-PCR analyses showing that knockdown of Smurf1 but not Smurf2 increased Ppar γ mRNA level in AML12 cells. (C) qRT-PCR analyses showing that knockdown of Smurf1 in AML12 cells increased expression of Fabp1 , Cd36 , Acacb , and Apoc3 in a PPARγ-dependent manner. (D) Fatty acid uptake in AML12 cells as measured by 3 H-palmitate incorporation ( n = 3). (E) Lipid synthesis in AML12 cells as measured by incorporation of 3 H-acetate into lipid ( n = 3). (F) In vivo fatty acid uptake after intraperitoneal injection of <t>BODIPY-FL-C16.</t> The BODIPY-FL-C16 accumulation in the liver, epididymal WAT, and skeletal muscle was normalized to tissue weight ( n = 8 per group). (G) Lipogenesis in primary hepatocytes as measured by the incorporation of 3 H-acetate into lipid ( n = 6 per group). Data are presented as mean ± SD; statistical significance of difference is indicated as * p
    Palmitic Acid, supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Merck & Co palmitic acid
    Smurf1 regulates fatty acid uptake and lipid synthesis through PPARγ. (A) Western blots showing that knockdown of Smurf1 but not Smurf2 in Hep3B and AML12 cells increased PPARγ protein level. (B) qRT-PCR analyses showing that knockdown of Smurf1 but not Smurf2 increased Ppar γ mRNA level in AML12 cells. (C) qRT-PCR analyses showing that knockdown of Smurf1 in AML12 cells increased expression of Fabp1 , Cd36 , Acacb , and Apoc3 in a PPARγ-dependent manner. (D) Fatty acid uptake in AML12 cells as measured by 3 H-palmitate incorporation ( n = 3). (E) Lipid synthesis in AML12 cells as measured by incorporation of 3 H-acetate into lipid ( n = 3). (F) In vivo fatty acid uptake after intraperitoneal injection of <t>BODIPY-FL-C16.</t> The BODIPY-FL-C16 accumulation in the liver, epididymal WAT, and skeletal muscle was normalized to tissue weight ( n = 8 per group). (G) Lipogenesis in primary hepatocytes as measured by the incorporation of 3 H-acetate into lipid ( n = 6 per group). Data are presented as mean ± SD; statistical significance of difference is indicated as * p
    Palmitic Acid, supplied by Merck & Co, used in various techniques. Bioz Stars score: 91/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Tokyo Chemical Industry palmitic acid
    Smurf1 regulates fatty acid uptake and lipid synthesis through PPARγ. (A) Western blots showing that knockdown of Smurf1 but not Smurf2 in Hep3B and AML12 cells increased PPARγ protein level. (B) qRT-PCR analyses showing that knockdown of Smurf1 but not Smurf2 increased Ppar γ mRNA level in AML12 cells. (C) qRT-PCR analyses showing that knockdown of Smurf1 in AML12 cells increased expression of Fabp1 , Cd36 , Acacb , and Apoc3 in a PPARγ-dependent manner. (D) Fatty acid uptake in AML12 cells as measured by 3 H-palmitate incorporation ( n = 3). (E) Lipid synthesis in AML12 cells as measured by incorporation of 3 H-acetate into lipid ( n = 3). (F) In vivo fatty acid uptake after intraperitoneal injection of <t>BODIPY-FL-C16.</t> The BODIPY-FL-C16 accumulation in the liver, epididymal WAT, and skeletal muscle was normalized to tissue weight ( n = 8 per group). (G) Lipogenesis in primary hepatocytes as measured by the incorporation of 3 H-acetate into lipid ( n = 6 per group). Data are presented as mean ± SD; statistical significance of difference is indicated as * p
    Palmitic Acid, supplied by Tokyo Chemical Industry, used in various techniques. Bioz Stars score: 92/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Moravek Biochemicals h palmitic acid
    Smurf1 regulates fatty acid uptake and lipid synthesis through PPARγ. (A) Western blots showing that knockdown of Smurf1 but not Smurf2 in Hep3B and AML12 cells increased PPARγ protein level. (B) qRT-PCR analyses showing that knockdown of Smurf1 but not Smurf2 increased Ppar γ mRNA level in AML12 cells. (C) qRT-PCR analyses showing that knockdown of Smurf1 in AML12 cells increased expression of Fabp1 , Cd36 , Acacb , and Apoc3 in a PPARγ-dependent manner. (D) Fatty acid uptake in AML12 cells as measured by 3 H-palmitate incorporation ( n = 3). (E) Lipid synthesis in AML12 cells as measured by incorporation of 3 H-acetate into lipid ( n = 3). (F) In vivo fatty acid uptake after intraperitoneal injection of <t>BODIPY-FL-C16.</t> The BODIPY-FL-C16 accumulation in the liver, epididymal WAT, and skeletal muscle was normalized to tissue weight ( n = 8 per group). (G) Lipogenesis in primary hepatocytes as measured by the incorporation of 3 H-acetate into lipid ( n = 6 per group). Data are presented as mean ± SD; statistical significance of difference is indicated as * p
    H Palmitic Acid, supplied by Moravek Biochemicals, used in various techniques. Bioz Stars score: 91/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher click it palmitic acid azide 15 azidopentadecanoic acid
    Chemical synthesis and characterization of AlexaFFA. ( a ) Schematic of the coupling reaction between Click-IT™ Alexa Fluor 647 DIBO Alkyne and <t>15-azidopentadecanoic</t> acid producing two regioisomers. ( b ) LC–MS (UV detection 254 nm) of Alexa Fluor 647 DIBO Alkyne (red line) and the coupling products (blue line) showing a doublet with an ionized mass of 1,444 + m/z (regioisomer 1,2) and a by-product with an ionized mass of 1685 + m/z. ( c ) Mass spectrometry of the extracted coupling products (m/z = 1,444 + ) showing closely eluting but distinct species.
    Click It Palmitic Acid Azide 15 Azidopentadecanoic Acid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Smurf1 regulates fatty acid uptake and lipid synthesis through PPARγ. (A) Western blots showing that knockdown of Smurf1 but not Smurf2 in Hep3B and AML12 cells increased PPARγ protein level. (B) qRT-PCR analyses showing that knockdown of Smurf1 but not Smurf2 increased Ppar γ mRNA level in AML12 cells. (C) qRT-PCR analyses showing that knockdown of Smurf1 in AML12 cells increased expression of Fabp1 , Cd36 , Acacb , and Apoc3 in a PPARγ-dependent manner. (D) Fatty acid uptake in AML12 cells as measured by 3 H-palmitate incorporation ( n = 3). (E) Lipid synthesis in AML12 cells as measured by incorporation of 3 H-acetate into lipid ( n = 3). (F) In vivo fatty acid uptake after intraperitoneal injection of BODIPY-FL-C16. The BODIPY-FL-C16 accumulation in the liver, epididymal WAT, and skeletal muscle was normalized to tissue weight ( n = 8 per group). (G) Lipogenesis in primary hepatocytes as measured by the incorporation of 3 H-acetate into lipid ( n = 6 per group). Data are presented as mean ± SD; statistical significance of difference is indicated as * p

    Journal: PLoS Biology

    Article Title: Non-proteolytic ubiquitin modification of PPARγ by Smurf1 protects the liver from steatosis

    doi: 10.1371/journal.pbio.3000091

    Figure Lengend Snippet: Smurf1 regulates fatty acid uptake and lipid synthesis through PPARγ. (A) Western blots showing that knockdown of Smurf1 but not Smurf2 in Hep3B and AML12 cells increased PPARγ protein level. (B) qRT-PCR analyses showing that knockdown of Smurf1 but not Smurf2 increased Ppar γ mRNA level in AML12 cells. (C) qRT-PCR analyses showing that knockdown of Smurf1 in AML12 cells increased expression of Fabp1 , Cd36 , Acacb , and Apoc3 in a PPARγ-dependent manner. (D) Fatty acid uptake in AML12 cells as measured by 3 H-palmitate incorporation ( n = 3). (E) Lipid synthesis in AML12 cells as measured by incorporation of 3 H-acetate into lipid ( n = 3). (F) In vivo fatty acid uptake after intraperitoneal injection of BODIPY-FL-C16. The BODIPY-FL-C16 accumulation in the liver, epididymal WAT, and skeletal muscle was normalized to tissue weight ( n = 8 per group). (G) Lipogenesis in primary hepatocytes as measured by the incorporation of 3 H-acetate into lipid ( n = 6 per group). Data are presented as mean ± SD; statistical significance of difference is indicated as * p

    Article Snippet: Briefly, mice were i.p. injected with BODIPY-FL-C16 (Life Technologies) after being fasted for 4 hours, then were euthanized at 5 hours after injection; liver, epididymal fat pad, and skeletal muscle were collected.

    Techniques: Western Blot, Quantitative RT-PCR, Expressing, In Vivo, Injection

    Chemical synthesis and characterization of AlexaFFA. ( a ) Schematic of the coupling reaction between Click-IT™ Alexa Fluor 647 DIBO Alkyne and 15-azidopentadecanoic acid producing two regioisomers. ( b ) LC–MS (UV detection 254 nm) of Alexa Fluor 647 DIBO Alkyne (red line) and the coupling products (blue line) showing a doublet with an ionized mass of 1,444 + m/z (regioisomer 1,2) and a by-product with an ionized mass of 1685 + m/z. ( c ) Mass spectrometry of the extracted coupling products (m/z = 1,444 + ) showing closely eluting but distinct species.

    Journal: Scientific Reports

    Article Title: A novel tracer for in vivo optical imaging of fatty acid metabolism in the heart and brown adipose tissue

    doi: 10.1038/s41598-020-68065-4

    Figure Lengend Snippet: Chemical synthesis and characterization of AlexaFFA. ( a ) Schematic of the coupling reaction between Click-IT™ Alexa Fluor 647 DIBO Alkyne and 15-azidopentadecanoic acid producing two regioisomers. ( b ) LC–MS (UV detection 254 nm) of Alexa Fluor 647 DIBO Alkyne (red line) and the coupling products (blue line) showing a doublet with an ionized mass of 1,444 + m/z (regioisomer 1,2) and a by-product with an ionized mass of 1685 + m/z. ( c ) Mass spectrometry of the extracted coupling products (m/z = 1,444 + ) showing closely eluting but distinct species.

    Article Snippet: SynthesisThe copper-free click reaction between 0.50 mg Click-IT Alexa Fluor 647 DIBO Alkyne (0.33 μmol, based on the molecular weight estimate provided by the vendor; Thermo Fisher Scientific C10408) and 10 molar equivalent of 15-azidopentadecanoic acid (0.94 mg, 3.3 μmol; Thermo Fisher Scientific C10265) was performed in 100 μL of methylsulfonylmethane (dimethyl sulfoxide; DMSO).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry