Journal: PLoS Pathogens

Article Title: Tat-exported peptidoglycan amidase-dependent cell division contributes to Salmonella Typhimurium fitness in the inflamed gut

doi: 10.1371/journal.ppat.1007391

Figure Lengend Snippet: Chain form confers attenuated resistance to deoxycholate. GFP-expressing S . Tm ssaV or ssaV amiA amiC mutants were grown in LB or LB plus 0.5 M NaCl, and subsequently were mixed with 1% deoxycholate (DOC), followed by incubation and treatment with PI. The resulting S . Tm cells were placed on a 1.5% agarose pad, sealed under a glass coverslip, and observed by fluorescence microscopy. (A) Representative fluorescence microscopy images. Scale bar, 20 μm. (B) Microscopy quantification of PI-stained cells. Three independent experiments were performed. ns, not significant; * P

Article Snippet: Tm strains grown to the logarithmic growth phase were diluted to 1×106 CFU per ml with different concentrations of magainin 2 (LKT Laboratories, Inc.) or deoxycholate (Nacalai tesque) in sterile LB broth, and incubated for 15 h at 37°C.

Techniques: Expressing, Incubation, Fluorescence, Microscopy, Staining

Journal: Molecules

Article Title: Chitosan-Coated Flexible Liposomes Magnify the Anticancer Activity and Bioavailability of Docetaxel: Impact on Composition

doi: 10.3390/molecules24020250

Figure Lengend Snippet: Zeta potentials of docetaxel (DXT)-loaded (5 mg/mL) liposomes and chitosomes. The data are expressed as means ± SD, n = 3. CL—conventional liposome; FL—flexible liposome; T—Tween-80; DP—dicetyl phosphate; NDC—sodium deoxycholate; C—chitosan-coated (0.5 mg/mL).

Article Snippet: Sodium deoxycholate, methanol, and acetonitrile (HPLC grade) were purchased from Fisher Scientific Co., (Hampton, NH, USA).

Techniques:

Journal: British Journal of Pharmacology

Article Title: Reduction of sodium deoxycholic acid-induced scratching behaviour by bradykinin B2 receptor antagonists

doi: 10.1038/sj.bjp.0702296

Figure Lengend Snippet: Effects of BK receptor antagonists on scratching induced by sodium deoxycholic acid. FR173657 (10–100 mg kg −1 ) was administered orally 1 h prior to the injection of 100 μg of sodium deoxycholic acid (DC). Hoe140 (30 μg site −1 ) or des-Arg 9 -[Leu 8 ]-BK (1 nmol site −1 ) was injected subcutaneously together with 100 μg of sodium deoxycholic acid. The frequency of scratching around the injection site by the fore- and hind-paws were counted for 60 min after the injection of sodium deoxycholic acid. Each value represents the mean±s.e.mean from 3–15 animals. Numbers in parenthesis are the numbers of mice used. Comparisons were made with the value from mice receiving 100 μg of sodium deoxycholic acid alone, indicated by an open column. * P ⩾0.05.

Article Snippet: Whereas the amounts of kinin released from the higher molecular weight fractions in the skin injected with sodium deoxycholic acid were almost the same as those in the skin injected with saline, the amount of kinin release from lower molecular weight fractions was markedly less for the skin injected with sodium deoxycholic acid than for that injected with saline.

Techniques: Injection, Mouse Assay

Journal: British Journal of Pharmacology

Article Title: Reduction of sodium deoxycholic acid-induced scratching behaviour by bradykinin B2 receptor antagonists

doi: 10.1038/sj.bjp.0702296

Figure Lengend Snippet: (a) Effects of kininase inhibitors on scratching induced by sodium deoxycholic acid. Phosphoramidon (30 μg site −1 ) or lisinopril (0.3 μg site −1 ) was subcutaneously injected together with 30 μg of sodium deoxycholic acid (DC) into the anterior part of the back of the mice. The frequency of scratching around the injection site by the fore- and hind-paws were counted for 60 min after the injection. Each value represents the mean±s.e.mean from 7–8 animals. Numbers in parenthesis indicate numbers of mice used. Comparisons were made with the value from mice receiving 30 μg of sodium deoxycholic acid alone, indicated by an open column. (b) Effects of kininase inhibitors on degradation of BK by skin extract or plasma of mice. Skin extract or plasma was incubated with BK for 10 min at 37°C in the presence or the absence of lisinopril (1 μg ml −1 ). Residual bradykinin in the mixture was measured using BK enzyme immunoassay kit. The ordinate indicates residual amounts of BK in the incubation mixture. Each column represents the mean±s.e.mean. ** P

Article Snippet: Whereas the amounts of kinin released from the higher molecular weight fractions in the skin injected with sodium deoxycholic acid were almost the same as those in the skin injected with saline, the amount of kinin release from lower molecular weight fractions was markedly less for the skin injected with sodium deoxycholic acid than for that injected with saline.

Techniques: Injection, Mouse Assay, Incubation, Enzyme-linked Immunosorbent Assay

Journal: British Journal of Pharmacology

Article Title: Reduction of sodium deoxycholic acid-induced scratching behaviour by bradykinin B2 receptor antagonists

doi: 10.1038/sj.bjp.0702296

Figure Lengend Snippet: Dose-response for the scratch-inducing effect of sodium deoxycholic acid. Ten to 100 μg of sodium deoxycholic acid was injected subcutaneously into the anterior part of the back of male ddY mice. The frequency of scratching around the injection site with the fore- and hind-paws was counted for 60 min after the injection. Each value represents mean±s.e.mean from 9–15 animals. Comparisons were made with the value from mice receiving 100 μl of saline indicated by an open column. * P

Article Snippet: Whereas the amounts of kinin released from the higher molecular weight fractions in the skin injected with sodium deoxycholic acid were almost the same as those in the skin injected with saline, the amount of kinin release from lower molecular weight fractions was markedly less for the skin injected with sodium deoxycholic acid than for that injected with saline.

Techniques: Injection, Mouse Assay

Journal: British Journal of Pharmacology

Article Title: Reduction of sodium deoxycholic acid-induced scratching behaviour by bradykinin B2 receptor antagonists

doi: 10.1038/sj.bjp.0702296

Figure Lengend Snippet: Scratching induced by sodium deoxycholic acid in the kininogen-deficient (B/N-Katholiek) and the normal (B/N-Kitasato) strain of rats. Sodium deoxycholic acid (DC; 100 μg site −1 ) was injected subcutaneously into the anterior part of the back of female Brown Norway Katholiek (B/N-Ka: kininogens-deficient strain) and Brown Norway Kitasato (B/N-Ki; the normal strain) rats. The frequency of scratching around the injection site by the fore- and hind-paws was counted for 120 min after the injection. Each value represents the mean±s.e.mean. The numbers in parenthesis indicate the numbers of rats used. The last two columns indicate the result following administered of FR173657 (10 mg kg −1 ) orally 1 h before the injection of sodium deoxycholic acid. Comparisons were made with the value from Brown Norway Kitasato rats receiving 100 μg of sodium deoxycholic acid shown in the left-hand. * P

Article Snippet: Whereas the amounts of kinin released from the higher molecular weight fractions in the skin injected with sodium deoxycholic acid were almost the same as those in the skin injected with saline, the amount of kinin release from lower molecular weight fractions was markedly less for the skin injected with sodium deoxycholic acid than for that injected with saline.

Techniques: Injection

Journal: British Journal of Pharmacology

Article Title: Reduction of sodium deoxycholic acid-induced scratching behaviour by bradykinin B2 receptor antagonists

doi: 10.1038/sj.bjp.0702296

Figure Lengend Snippet: Vascular permeability increase due to sodium deoxycholic acid in mouse skin. Mice anaesthetized with Nembutal (50 mg kg −1 ) were administered pontamine sky blue (100 kg mg −1 ) intravenously followed by s.c. injections of sodium deoxycholic acid (10–1000 μg site −1 ) intravenously followed by s.c. injections of sodium deoxycholic acid (10–1000 μg site −1 ), BK (0.3–10 nmol site −1 ), or saline alone (100 μl) into the dorsal skin. At 30 min after the injection, mice were sacrificed to quantify the amounts of dye that leaked into the skin as described in the text. Each value represents the mean±s.e.mean. Numbers in parenthesis indicate the numbers of experiments. Comparisons were made with the value from mice receiving 100 μl of saline. ** P

Article Snippet: Whereas the amounts of kinin released from the higher molecular weight fractions in the skin injected with sodium deoxycholic acid were almost the same as those in the skin injected with saline, the amount of kinin release from lower molecular weight fractions was markedly less for the skin injected with sodium deoxycholic acid than for that injected with saline.

Techniques: Permeability, Mouse Assay, Injection

Journal: British Journal of Pharmacology

Article Title: Reduction of sodium deoxycholic acid-induced scratching behaviour by bradykinin B2 receptor antagonists

doi: 10.1038/sj.bjp.0702296

Figure Lengend Snippet: Time course of scratching after s.c. injection of sodium deoxycholic acid. Sodium deoxycholic acid (100 μg site −1 ) was injected subcutaneously into the anterior part of the back of male ddY mice under light ether anaesthesia. Each mouse was then placed separately in a cage so that its behaviour could be recorded with an unmanned video camera. Frequency of scratching around the injection site with the fore- and hind-paws was counted for every 5-min period over a 90 min duration after the injection. Each value represents mean±s.e.mean from 9–15 animals.

Article Snippet: Whereas the amounts of kinin released from the higher molecular weight fractions in the skin injected with sodium deoxycholic acid were almost the same as those in the skin injected with saline, the amount of kinin release from lower molecular weight fractions was markedly less for the skin injected with sodium deoxycholic acid than for that injected with saline.

Techniques: Injection, Mouse Assay

Journal: British Journal of Pharmacology

Article Title: Reduction of sodium deoxycholic acid-induced scratching behaviour by bradykinin B2 receptor antagonists

doi: 10.1038/sj.bjp.0702296

Figure Lengend Snippet: Effects of kallikrein inhibitors on scratching induced by sodium deoxycholic acid. Aprotinin (10 mg kg −1 ) was administered intravenously 5 min prior to the injection of 100 μg of sodium deoxycholic acid (DC). Soybean trypsin inhibitor (SBTI; 100 μg site −1 ) was subcutaneously injected together with 100 μg of sodium deoxycholic acid. The frequency of scratching were counted for 60 min after the injection of sodium deoxycholic acid. Each value represents the mean±s.e.mean from 5–15 animals. Numbers in parenthesis indicate the numbers of mice used. Comparisons were made with the value from mice receiving 100 μg of sodium deoxycholic acid alone, indicated by an open column. * P

Article Snippet: Whereas the amounts of kinin released from the higher molecular weight fractions in the skin injected with sodium deoxycholic acid were almost the same as those in the skin injected with saline, the amount of kinin release from lower molecular weight fractions was markedly less for the skin injected with sodium deoxycholic acid than for that injected with saline.

Techniques: Injection, Mouse Assay

Journal: Biochemistry

Article Title: NMR characterization of the interaction of the Salmonella type III secretion system protein SipD and bile salts

doi: 10.1021/bi100335u

Figure Lengend Snippet: Weighted chemical shift difference (Δδ HN ) of the 1 H and 15 N resonances of SipD 39–343 when titrated with (A) deoxycholate, (B) taurodeoxycholate and (C) chenodeoxycholate (Δδ HN = [½ (δ H 2 + 1/25δ

Article Snippet: For NMR chemical shift mapping, 2D 1 H-15 N TROSY-HSQC ( ) spectra were acquired using 15 N-labeled SipD39–343 that was titrated with varying amounts of deoxycholate (Amresco), chenodeoxycholate (Sigma), taurodeoxycholate (Sigma) or cholate hydrate (Sigma).

Techniques:

Journal: Biochemistry

Article Title: NMR characterization of the interaction of the Salmonella type III secretion system protein SipD and bile salts

doi: 10.1021/bi100335u

Figure Lengend Snippet: (A) Overlay of four 2D 1 H- 15 N TROSY spectra of [ 2 H, 15 N, 13 C]-labeled SipD 39–343 with increasing amounts of deoxycholate. Some of the assignments are indicated as well as the noise peaks (*). (B) Expanded regions of the 2D 1 H- 15 N TROSY spectra for

Article Snippet: For NMR chemical shift mapping, 2D 1 H-15 N TROSY-HSQC ( ) spectra were acquired using 15 N-labeled SipD39–343 that was titrated with varying amounts of deoxycholate (Amresco), chenodeoxycholate (Sigma), taurodeoxycholate (Sigma) or cholate hydrate (Sigma).

Techniques: Labeling

Journal: Biochemistry

Article Title: NMR characterization of the interaction of the Salmonella type III secretion system protein SipD and bile salts

doi: 10.1021/bi100335u

Figure Lengend Snippet: Ribbon representation of the crystal structure of SipD 39–343 (to be reported elsewhere). The side chains of residues that showed significant chemical shift perturbation (Δδ HN > 0.03) with deoxycholate were colored red (legend:

Article Snippet: For NMR chemical shift mapping, 2D 1 H-15 N TROSY-HSQC ( ) spectra were acquired using 15 N-labeled SipD39–343 that was titrated with varying amounts of deoxycholate (Amresco), chenodeoxycholate (Sigma), taurodeoxycholate (Sigma) or cholate hydrate (Sigma).

Techniques: