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    Illumina Inc small rna seq srna seq libraries
    Small Rna Seq Srna Seq Libraries, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/small rna seq srna seq libraries/product/Illumina Inc
    Average 88 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    small rna seq srna seq libraries - by Bioz Stars, 2020-07
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    99
    Illumina Inc truseq small rna seq small rna libraries
    a Average percentage of reads mapped to the human transcriptome to <t>RNA</t> biotypes for <t>Illumina</t> TruSeq and BiooScientific NEXTFlex for the plasma samples. The percentages presented here are averaged over the 6 different volumes for each kit respectively. b PCA plot of the plasma samples show clustering of the samples by kit-type and not by input volume. c Number of miRNAs detected at three expression thresholds: > 1 read per million mapped to the genome (RPM), > 10 RPM and > 100 RPM for all the plasma samples for Illumina TruSeq and BiooScientific NEXTFlex
    Truseq Small Rna Seq Small Rna Libraries, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq small rna seq small rna libraries/product/Illumina Inc
    Average 99 stars, based on 84 article reviews
    Price from $9.99 to $1999.99
    truseq small rna seq small rna libraries - by Bioz Stars, 2020-07
    99/100 stars
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    93
    Illumina Inc srna libraries
    Small <t>RNA</t> expression profiles detected with RNA-seq. The panels include the known sRNAs GlmZ, CyaR, MicF, McaS and RyhB, a novel <t>sRNA</t> with clearly defined coordinates, ES043, a novel sRNA with less defined coordinates, ES067, and a novel sRNA with vaguely defined coordinates and low expression level, ES116. The y-axes of the plots denote read coverage at each nucleotide position for the pooled fermentation and stress samples. The x-axes denote the genomic positions according to the E. coli K-12 MG1655 genome coordinates (NC_000913.2). Legend: orange lines, expression signal; dashed green lines, sRNA coordinates; dashed blue lines, intergenic region coordinates; arrows denote directions of flanking genes (blue) and sRNAs (green)
    Srna Libraries, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 1188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/srna libraries/product/Illumina Inc
    Average 93 stars, based on 1188 article reviews
    Price from $9.99 to $1999.99
    srna libraries - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    99
    Illumina Inc tru seq srna kit
    Small <t>RNA</t> expression profiles detected with RNA-seq. The panels include the known sRNAs GlmZ, CyaR, MicF, McaS and RyhB, a novel <t>sRNA</t> with clearly defined coordinates, ES043, a novel sRNA with less defined coordinates, ES067, and a novel sRNA with vaguely defined coordinates and low expression level, ES116. The y-axes of the plots denote read coverage at each nucleotide position for the pooled fermentation and stress samples. The x-axes denote the genomic positions according to the E. coli K-12 MG1655 genome coordinates (NC_000913.2). Legend: orange lines, expression signal; dashed green lines, sRNA coordinates; dashed blue lines, intergenic region coordinates; arrows denote directions of flanking genes (blue) and sRNAs (green)
    Tru Seq Srna Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tru seq srna kit/product/Illumina Inc
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    tru seq srna kit - by Bioz Stars, 2020-07
    99/100 stars
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    93
    Illumina Inc ultra small rna sample library prep kit
    Small <t>RNA</t> expression profiles detected with RNA-seq. The panels include the known sRNAs GlmZ, CyaR, MicF, McaS and RyhB, a novel <t>sRNA</t> with clearly defined coordinates, ES043, a novel sRNA with less defined coordinates, ES067, and a novel sRNA with vaguely defined coordinates and low expression level, ES116. The y-axes of the plots denote read coverage at each nucleotide position for the pooled fermentation and stress samples. The x-axes denote the genomic positions according to the E. coli K-12 MG1655 genome coordinates (NC_000913.2). Legend: orange lines, expression signal; dashed green lines, sRNA coordinates; dashed blue lines, intergenic region coordinates; arrows denote directions of flanking genes (blue) and sRNAs (green)
    Ultra Small Rna Sample Library Prep Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultra small rna sample library prep kit/product/Illumina Inc
    Average 93 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    ultra small rna sample library prep kit - by Bioz Stars, 2020-07
    93/100 stars
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    94
    Illumina Inc truseq small rna library prep
    Small <t>RNA</t> expression profiles detected with RNA-seq. The panels include the known sRNAs GlmZ, CyaR, MicF, McaS and RyhB, a novel <t>sRNA</t> with clearly defined coordinates, ES043, a novel sRNA with less defined coordinates, ES067, and a novel sRNA with vaguely defined coordinates and low expression level, ES116. The y-axes of the plots denote read coverage at each nucleotide position for the pooled fermentation and stress samples. The x-axes denote the genomic positions according to the E. coli K-12 MG1655 genome coordinates (NC_000913.2). Legend: orange lines, expression signal; dashed green lines, sRNA coordinates; dashed blue lines, intergenic region coordinates; arrows denote directions of flanking genes (blue) and sRNAs (green)
    Truseq Small Rna Library Prep, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq small rna library prep/product/Illumina Inc
    Average 94 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    truseq small rna library prep - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    Image Search Results


    a Average percentage of reads mapped to the human transcriptome to RNA biotypes for Illumina TruSeq and BiooScientific NEXTFlex for the plasma samples. The percentages presented here are averaged over the 6 different volumes for each kit respectively. b PCA plot of the plasma samples show clustering of the samples by kit-type and not by input volume. c Number of miRNAs detected at three expression thresholds: > 1 read per million mapped to the genome (RPM), > 10 RPM and > 100 RPM for all the plasma samples for Illumina TruSeq and BiooScientific NEXTFlex

    Journal: BMC Genomics

    Article Title: Evaluation of commercially available small RNASeq library preparation kits using low input RNA

    doi: 10.1186/s12864-018-4726-6

    Figure Lengend Snippet: a Average percentage of reads mapped to the human transcriptome to RNA biotypes for Illumina TruSeq and BiooScientific NEXTFlex for the plasma samples. The percentages presented here are averaged over the 6 different volumes for each kit respectively. b PCA plot of the plasma samples show clustering of the samples by kit-type and not by input volume. c Number of miRNAs detected at three expression thresholds: > 1 read per million mapped to the genome (RPM), > 10 RPM and > 100 RPM for all the plasma samples for Illumina TruSeq and BiooScientific NEXTFlex

    Article Snippet: Small RNA libraries were generated using NEB Next Small RNA Library Prep Set for Illumina (NEB #E7330S).

    Techniques: Expressing

    MicroRNA expression positively correlates with mRNA target repression. A) Schematic representing the vector used in repression assays containing Renilla luciferase gene, with a single microRNA targeting sequence incorporated into the 3′UTR, and the firefly luciferase gene used as the transfection control. B) Relationship of microRNA expression and target repression for 32 mature microRNAs. Repression of target mRNA was determined by dual luciferase reporter assay (see Methods). MicroRNA expression levels were estimated as normalized read counts (per million mapped to the genome – RPM) by Illumina small RNA deep sequencing on two independent samples of S2-DRSC cells – points represent average read counts. Filled points represent mature microRNA sequences and open points represent microRNA* sequences (defined as the less abundant arm when the ratio of arm abundance exceeds 4∶1). Error bars represent standard deviation. The line of best fit was estimated by linear regression.

    Journal: PLoS ONE

    Article Title: Target Repression Induced by Endogenous microRNAs: Large Differences, Small Effects

    doi: 10.1371/journal.pone.0104286

    Figure Lengend Snippet: MicroRNA expression positively correlates with mRNA target repression. A) Schematic representing the vector used in repression assays containing Renilla luciferase gene, with a single microRNA targeting sequence incorporated into the 3′UTR, and the firefly luciferase gene used as the transfection control. B) Relationship of microRNA expression and target repression for 32 mature microRNAs. Repression of target mRNA was determined by dual luciferase reporter assay (see Methods). MicroRNA expression levels were estimated as normalized read counts (per million mapped to the genome – RPM) by Illumina small RNA deep sequencing on two independent samples of S2-DRSC cells – points represent average read counts. Filled points represent mature microRNA sequences and open points represent microRNA* sequences (defined as the less abundant arm when the ratio of arm abundance exceeds 4∶1). Error bars represent standard deviation. The line of best fit was estimated by linear regression.

    Article Snippet: Small RNA library preparation, using the Illumina TruSeq Small RNA Kit, and Illumina sequencing were performed by GATC using a HiSeq 2000 machine.

    Techniques: Expressing, Plasmid Preparation, Luciferase, Sequencing, Transfection, Reporter Assay, Standard Deviation

    Correlation between microRNA levels in the cell and in Ago-1 immunoprecipitation experiments. MicroRNA expression levels were estimated by Illumina deep sequencing on two independent samples of size-fractionated RNA from S2-DRSC cells for both cellular and Ago-1 pull-down. Filled circles represent the microRNAs chosen for further analysis. The x-axis shows log 10 normalized cellular read counts expressed as average reads per million, for two samples. The y-axis shows log 10 normalized Ago-1 pull-down read counts expressed in reads per million, for two independent samples. Line of best fit was produced by linear regression.

    Journal: PLoS ONE

    Article Title: Target Repression Induced by Endogenous microRNAs: Large Differences, Small Effects

    doi: 10.1371/journal.pone.0104286

    Figure Lengend Snippet: Correlation between microRNA levels in the cell and in Ago-1 immunoprecipitation experiments. MicroRNA expression levels were estimated by Illumina deep sequencing on two independent samples of size-fractionated RNA from S2-DRSC cells for both cellular and Ago-1 pull-down. Filled circles represent the microRNAs chosen for further analysis. The x-axis shows log 10 normalized cellular read counts expressed as average reads per million, for two samples. The y-axis shows log 10 normalized Ago-1 pull-down read counts expressed in reads per million, for two independent samples. Line of best fit was produced by linear regression.

    Article Snippet: Small RNA library preparation, using the Illumina TruSeq Small RNA Kit, and Illumina sequencing were performed by GATC using a HiSeq 2000 machine.

    Techniques: Immunoprecipitation, Expressing, Sequencing, Produced

    Small RNA expression profiles detected with RNA-seq. The panels include the known sRNAs GlmZ, CyaR, MicF, McaS and RyhB, a novel sRNA with clearly defined coordinates, ES043, a novel sRNA with less defined coordinates, ES067, and a novel sRNA with vaguely defined coordinates and low expression level, ES116. The y-axes of the plots denote read coverage at each nucleotide position for the pooled fermentation and stress samples. The x-axes denote the genomic positions according to the E. coli K-12 MG1655 genome coordinates (NC_000913.2). Legend: orange lines, expression signal; dashed green lines, sRNA coordinates; dashed blue lines, intergenic region coordinates; arrows denote directions of flanking genes (blue) and sRNAs (green)

    Journal: BMC Genomics

    Article Title: Differential expression of small RNAs under chemical stress and fed-batch fermentation in E. coli

    doi: 10.1186/s12864-015-2231-8

    Figure Lengend Snippet: Small RNA expression profiles detected with RNA-seq. The panels include the known sRNAs GlmZ, CyaR, MicF, McaS and RyhB, a novel sRNA with clearly defined coordinates, ES043, a novel sRNA with less defined coordinates, ES067, and a novel sRNA with vaguely defined coordinates and low expression level, ES116. The y-axes of the plots denote read coverage at each nucleotide position for the pooled fermentation and stress samples. The x-axes denote the genomic positions according to the E. coli K-12 MG1655 genome coordinates (NC_000913.2). Legend: orange lines, expression signal; dashed green lines, sRNA coordinates; dashed blue lines, intergenic region coordinates; arrows denote directions of flanking genes (blue) and sRNAs (green)

    Article Snippet: Finally sRNA libraries were constructed using the TruSeq Small RNA Sample Preparation Kit (Illumina) with a few modifications.

    Techniques: RNA Expression, RNA Sequencing Assay, Expressing

    Features of the 253 novel small RNA transcripts detected in this study in relation to previously described sRNAs. Distribution of small RNAs on the E. coli MG1655 genome ( a ), where the novel sRNAs detected in this study are depicted on the inner ring, the known annotated sRNAs on the middle ring and the sRNAs detected in [ 17 ] on the outer ring. The transcript lengths and expression levels for the novel small RNAs compared to the known annotated and those predicted by Shinhara et al. [ 17 ], are shown in ( b ) and ( c ), respectively. Expression levels are presented as cumulative Mean Expression Values (MEV) defined as total stress and fermentation library reads for each sRNA divided by sRNA length

    Journal: BMC Genomics

    Article Title: Differential expression of small RNAs under chemical stress and fed-batch fermentation in E. coli

    doi: 10.1186/s12864-015-2231-8

    Figure Lengend Snippet: Features of the 253 novel small RNA transcripts detected in this study in relation to previously described sRNAs. Distribution of small RNAs on the E. coli MG1655 genome ( a ), where the novel sRNAs detected in this study are depicted on the inner ring, the known annotated sRNAs on the middle ring and the sRNAs detected in [ 17 ] on the outer ring. The transcript lengths and expression levels for the novel small RNAs compared to the known annotated and those predicted by Shinhara et al. [ 17 ], are shown in ( b ) and ( c ), respectively. Expression levels are presented as cumulative Mean Expression Values (MEV) defined as total stress and fermentation library reads for each sRNA divided by sRNA length

    Article Snippet: Finally sRNA libraries were constructed using the TruSeq Small RNA Sample Preparation Kit (Illumina) with a few modifications.

    Techniques: Expressing

    a Average percentage of reads mapped to the human transcriptome to RNA biotypes for Illumina TruSeq and BiooScientific NEXTFlex for the plasma samples. The percentages presented here are averaged over the 6 different volumes for each kit respectively. b PCA plot of the plasma samples show clustering of the samples by kit-type and not by input volume. c Number of miRNAs detected at three expression thresholds: > 1 read per million mapped to the genome (RPM), > 10 RPM and > 100 RPM for all the plasma samples for Illumina TruSeq and BiooScientific NEXTFlex

    Journal: BMC Genomics

    Article Title: Evaluation of commercially available small RNASeq library preparation kits using low input RNA

    doi: 10.1186/s12864-018-4726-6

    Figure Lengend Snippet: a Average percentage of reads mapped to the human transcriptome to RNA biotypes for Illumina TruSeq and BiooScientific NEXTFlex for the plasma samples. The percentages presented here are averaged over the 6 different volumes for each kit respectively. b PCA plot of the plasma samples show clustering of the samples by kit-type and not by input volume. c Number of miRNAs detected at three expression thresholds: > 1 read per million mapped to the genome (RPM), > 10 RPM and > 100 RPM for all the plasma samples for Illumina TruSeq and BiooScientific NEXTFlex

    Article Snippet: Illumina TruSeq small RNA library preparation Small RNA libraries were generated using Illumina TruSeq Small RNA Sample kit (RS-200-0048; Illumina).

    Techniques: Expressing

    Schematic of study design. Tissue RNA from brain, liver and placenta were sequenced at two sites at two input amounts (1 μg and 10 ng) using three different RNA sequencing kits (Illumina TruSeq, NEB Next and BiooScientific NEXTFlex). RNA from plasma samples at 5 different input volumes (200 μL – 5 mL) were sequenced at Site 1 using only TruSeq and BiooScientific. The green arrow depicts the flow of one of the tissue samples – brain using NEB Next and the red arrows, the plasma samples. The RNASeq results from the tissue samples were then validated using three different platforms (qPCR, EdgeSeq performed by Site1 and Fireplex performed by Site2). For a full list of samples sequenced, please refer to Additional file 1 : Table S1

    Journal: BMC Genomics

    Article Title: Evaluation of commercially available small RNASeq library preparation kits using low input RNA

    doi: 10.1186/s12864-018-4726-6

    Figure Lengend Snippet: Schematic of study design. Tissue RNA from brain, liver and placenta were sequenced at two sites at two input amounts (1 μg and 10 ng) using three different RNA sequencing kits (Illumina TruSeq, NEB Next and BiooScientific NEXTFlex). RNA from plasma samples at 5 different input volumes (200 μL – 5 mL) were sequenced at Site 1 using only TruSeq and BiooScientific. The green arrow depicts the flow of one of the tissue samples – brain using NEB Next and the red arrows, the plasma samples. The RNASeq results from the tissue samples were then validated using three different platforms (qPCR, EdgeSeq performed by Site1 and Fireplex performed by Site2). For a full list of samples sequenced, please refer to Additional file 1 : Table S1

    Article Snippet: Illumina TruSeq small RNA library preparation Small RNA libraries were generated using Illumina TruSeq Small RNA Sample kit (RS-200-0048; Illumina).

    Techniques: RNA Sequencing Assay, Flow Cytometry, Real-time Polymerase Chain Reaction

    Small RNA analysis performed by using the Agilent 2100 Bioanalyzer and and the sequencing library preparation using Illumina TruSeq Small RNA kit: a miRNA/sRNA ratio: Ratio of miRNA molecules expressed as a portion of all small RNA molecules (%) detected with the Agilent 2100 Bioanalyzer. b Electronic gel image of small RNA library obtained using Illumina TruSeq Small RNA kit for the unpooled samples where carrier was added to the TRIzol extraction procedure

    Journal: Molecular Biology Reports

    Article Title: Identification of extracellular miRNA in archived serum samples by next-generation sequencing from RNA extracted using multiple methods

    doi: 10.1007/s11033-016-4043-6

    Figure Lengend Snippet: Small RNA analysis performed by using the Agilent 2100 Bioanalyzer and and the sequencing library preparation using Illumina TruSeq Small RNA kit: a miRNA/sRNA ratio: Ratio of miRNA molecules expressed as a portion of all small RNA molecules (%) detected with the Agilent 2100 Bioanalyzer. b Electronic gel image of small RNA library obtained using Illumina TruSeq Small RNA kit for the unpooled samples where carrier was added to the TRIzol extraction procedure

    Article Snippet: The small RNA library preparation was carried out using Illumina TruSeq small RNA kit and sequencing was carried out using Illumina platform.

    Techniques: Sequencing

    miRNA detection and bias. Percentages of Miltenyi Biotec miRXplore miRNA detected above 5 CPM in MUR ( A ) and in MUR-D ( B ) are shown. Percentages and amplitude of Miltenyi Biotec miRXplore miRNA detected that deviated from the median are shown in MUR ( C ) or MUR-D ( D ). The darkest shade is within 2-fold of the median; the medium shade is 2–10-fold either up or down vs. the expected value; the lightest shade is > 10× either increased or decreased. Percentages of reads increased > 10× from median in MUR ( E ) or MUR-D ( F ). CLO-S and C, Takara Bio (Clontech) SMARTer smRNA-Seq Kit; DIA and D, Diagenode CATS Small RNA-Seq Kit; ILMN and I, Illumina TruSeq Small RNA Library Prep Kit; LEX and L, Lexogen Small RNA-Seq Library Prep Kit; Nano, NanoString nCounter miRNA Expression Assay; NEB and N, New England Biolabs NEBNext Small RNA Library Prep Set; PEB and P, PerkinElmer NextFlex Small RNA-Seq Kit v.3; QIA and Q, Qiagen QIAseq miRNA Library Kit; SOM and S, Somagenics RealSeq-AC miRNA Library Kit; TRI and T, Trilink CleanTag Small RNA Library Prep Kit.

    Journal: Journal of Biomolecular Techniques : JBT

    Article Title: Multisite Evaluation of Next-Generation Methods for Small RNA Quantification

    doi: 10.7171/jbt.20-3102-001

    Figure Lengend Snippet: miRNA detection and bias. Percentages of Miltenyi Biotec miRXplore miRNA detected above 5 CPM in MUR ( A ) and in MUR-D ( B ) are shown. Percentages and amplitude of Miltenyi Biotec miRXplore miRNA detected that deviated from the median are shown in MUR ( C ) or MUR-D ( D ). The darkest shade is within 2-fold of the median; the medium shade is 2–10-fold either up or down vs. the expected value; the lightest shade is > 10× either increased or decreased. Percentages of reads increased > 10× from median in MUR ( E ) or MUR-D ( F ). CLO-S and C, Takara Bio (Clontech) SMARTer smRNA-Seq Kit; DIA and D, Diagenode CATS Small RNA-Seq Kit; ILMN and I, Illumina TruSeq Small RNA Library Prep Kit; LEX and L, Lexogen Small RNA-Seq Library Prep Kit; Nano, NanoString nCounter miRNA Expression Assay; NEB and N, New England Biolabs NEBNext Small RNA Library Prep Set; PEB and P, PerkinElmer NextFlex Small RNA-Seq Kit v.3; QIA and Q, Qiagen QIAseq miRNA Library Kit; SOM and S, Somagenics RealSeq-AC miRNA Library Kit; TRI and T, Trilink CleanTag Small RNA Library Prep Kit.

    Article Snippet: Most of the kits tested, including Illumina TruSeq Small RNA Library Prep Kit, Lexogen Small RNA-Seq Library Prep Kit, New England Biolabs NEBNext Small RNA Library Prep Set, PerkinElmer (formerly Bioo Scientific) NextFlex Small RNA-Seq Kit v.3, Qiagen QIAseq miRNA Library Kit, and Trilink CleanTag Small RNA Library Prep Kit all use variants of this methodology.

    Techniques: RNA Sequencing Assay, Expressing

    Study design and workflow metrics. A ) Schematic of study design is shown. MUR, MUR-D, and HBR were processed using 9 different smRNA profiling methods at 4 sites each. The general methodologies included sequential ligation (Illumina, TriLink, Qiagen, NEB, PerkinElmer, Lexogen), template switching (Takara, Diagenode), circularization (Somagenics), and NanoString probe-based hybridization. B ) Total start-to-finish preparation time for each kit as reported by sites. C ) Mean “ease of use” reported by each site (scale: 1 = uncomfortable, 5 = comfortable). D ) Library preparation success rates. Light color blocks are successfully produced libraries. Dark colors are failed libraries. CLO and CLO-S, Takara Bio (Clontech) SMARTer smRNA-Seq Kit; DIA, Diagenode CATS Small RNA-Seq Kit; ILL and ILMN, Illumina TruSeq Small RNA Library Prep Kit; LEX, Lexogen Small RNA-Seq Library Prep Kit; NANO, NanoString nCounter miRNA Expression Assay; NEB, New England Biolabs NEBNext Small RNA Library Prep Set; PEB, PerkinElmer NextFlex Small RNA-Seq Kit v.3; QIA, Qiagen QIAseq miRNA Library Kit; SOM, Somagenics RealSeq-AC miRNA Library Kit; TRI, Trilink CleanTag Small RNA Library Prep Kit.

    Journal: Journal of Biomolecular Techniques : JBT

    Article Title: Multisite Evaluation of Next-Generation Methods for Small RNA Quantification

    doi: 10.7171/jbt.20-3102-001

    Figure Lengend Snippet: Study design and workflow metrics. A ) Schematic of study design is shown. MUR, MUR-D, and HBR were processed using 9 different smRNA profiling methods at 4 sites each. The general methodologies included sequential ligation (Illumina, TriLink, Qiagen, NEB, PerkinElmer, Lexogen), template switching (Takara, Diagenode), circularization (Somagenics), and NanoString probe-based hybridization. B ) Total start-to-finish preparation time for each kit as reported by sites. C ) Mean “ease of use” reported by each site (scale: 1 = uncomfortable, 5 = comfortable). D ) Library preparation success rates. Light color blocks are successfully produced libraries. Dark colors are failed libraries. CLO and CLO-S, Takara Bio (Clontech) SMARTer smRNA-Seq Kit; DIA, Diagenode CATS Small RNA-Seq Kit; ILL and ILMN, Illumina TruSeq Small RNA Library Prep Kit; LEX, Lexogen Small RNA-Seq Library Prep Kit; NANO, NanoString nCounter miRNA Expression Assay; NEB, New England Biolabs NEBNext Small RNA Library Prep Set; PEB, PerkinElmer NextFlex Small RNA-Seq Kit v.3; QIA, Qiagen QIAseq miRNA Library Kit; SOM, Somagenics RealSeq-AC miRNA Library Kit; TRI, Trilink CleanTag Small RNA Library Prep Kit.

    Article Snippet: Most of the kits tested, including Illumina TruSeq Small RNA Library Prep Kit, Lexogen Small RNA-Seq Library Prep Kit, New England Biolabs NEBNext Small RNA Library Prep Set, PerkinElmer (formerly Bioo Scientific) NextFlex Small RNA-Seq Kit v.3, Qiagen QIAseq miRNA Library Kit, and Trilink CleanTag Small RNA Library Prep Kit all use variants of this methodology.

    Techniques: Ligation, Hybridization, Produced, RNA Sequencing Assay, Expressing

    Relative expression of miRNAs. A ) Correlation scatter plots of miRNAs detected in MUR and MUR-D for the QIA method, with miRNAs scored in log2 mapped reads per million. B ) Correlation scatter plot of miRNAs detected in MUR-D using LEX vs. ILL kits, with miRNAs scored in log2 mapped reads per million. C ) Unsupervised hierarchical clustering heatmap of log2 transformed CPM across all methods. C (prep kit), Takara Bio (Clontech) SMARTer smRNA-Seq Kit; C (prep type), circularization; D, Diagenode CATS Small RNA-Seq Kit; ILL and I, Illumina TruSeq Small RNA Library Prep Kit; L (prep type), sequential ligation; LEX and L (prep kit), Lexogen Small RNA-Seq Library Prep Kit; N, New England Biolabs NEBNext Small RNA Library Prep Set; Nano, NanoString nCounter miRNA Expression Assay; NO, No size selection; P, PerkinElmer NextFlex Small RNA-Seq Kit v.3; QIA and Q, Qiagen QIAseq miRNA Library Kit; S, Somagenics RealSeq-AC miRNA Library Kit; SAGE, pippin prep; SPRI, solid phase reversible immobilization; T (prep kit), Trilink CleanTag Small Library Prep Kit; T (prep type), template switching.

    Journal: Journal of Biomolecular Techniques : JBT

    Article Title: Multisite Evaluation of Next-Generation Methods for Small RNA Quantification

    doi: 10.7171/jbt.20-3102-001

    Figure Lengend Snippet: Relative expression of miRNAs. A ) Correlation scatter plots of miRNAs detected in MUR and MUR-D for the QIA method, with miRNAs scored in log2 mapped reads per million. B ) Correlation scatter plot of miRNAs detected in MUR-D using LEX vs. ILL kits, with miRNAs scored in log2 mapped reads per million. C ) Unsupervised hierarchical clustering heatmap of log2 transformed CPM across all methods. C (prep kit), Takara Bio (Clontech) SMARTer smRNA-Seq Kit; C (prep type), circularization; D, Diagenode CATS Small RNA-Seq Kit; ILL and I, Illumina TruSeq Small RNA Library Prep Kit; L (prep type), sequential ligation; LEX and L (prep kit), Lexogen Small RNA-Seq Library Prep Kit; N, New England Biolabs NEBNext Small RNA Library Prep Set; Nano, NanoString nCounter miRNA Expression Assay; NO, No size selection; P, PerkinElmer NextFlex Small RNA-Seq Kit v.3; QIA and Q, Qiagen QIAseq miRNA Library Kit; S, Somagenics RealSeq-AC miRNA Library Kit; SAGE, pippin prep; SPRI, solid phase reversible immobilization; T (prep kit), Trilink CleanTag Small Library Prep Kit; T (prep type), template switching.

    Article Snippet: Most of the kits tested, including Illumina TruSeq Small RNA Library Prep Kit, Lexogen Small RNA-Seq Library Prep Kit, New England Biolabs NEBNext Small RNA Library Prep Set, PerkinElmer (formerly Bioo Scientific) NextFlex Small RNA-Seq Kit v.3, Qiagen QIAseq miRNA Library Kit, and Trilink CleanTag Small RNA Library Prep Kit all use variants of this methodology.

    Techniques: Expressing, Transformation Assay, RNA Sequencing Assay, Ligation, Selection