small hairpin rna shrna lentiviral particles Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Santa Cruz Biotechnology ucp2
    Upregulation of <t>UCP2</t> by TRPV1 activation attenuates hyperglycemia-induced ROS production in ECs. The increased metabolism of glucose due to intracellular hyperglycemia leads to the overproduction of NADH, a critical component of the superoxide-generating mechanism in endothelial cells. Upregulation of mitochondrial UCP2 in response to elevated superoxide levels plays an active role in the feedback regulation of reactive oxygen species production that is associated with chronic oxidative stress. Activation of the endothelial TRPV1 channel in endothelial cells by dietary capsaicin mediates the phosphorylation of PKA and upregulates UCP2, thus inhibiting the activity of NADPH and decreasing ROS production in ECs.
    Ucp2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ucp2/product/Santa Cruz Biotechnology
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ucp2 - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    90
    Santa Cruz Biotechnology lentiviral particles
    Endogenous TG2 affects the morphological appearance of fibronectin. Fibronectin (FN) aggregates on laminin (LAM) coated plastic were studied after removal of astrocytes that were allowed to produce extracellular matrix during 48 h of TNF-α+IL-1β treatment ( a , b ). Representative blots of four independent experiments are shown. Each bar represents the mean + standard error of the mean of signal intensities from blots of four separate experiments that were calculated as average percentage compared to control. Primary rat astrocytes were treated with TNF-α+IL-1β for 48 h plated on laminin coated plastic. Immunocytochemical double stainings showed immunoreactivity of fibronectin (FN, red) and TG2 (green) in astrocytes ( c , d , e , and f ). Astrocytes were co-incubated with TNF-α+IL-1β and the TG2-specific inhibitor ERW1041E (10 μM for 48 h) or DMSO ( d ) or treated with TNF-α+IL-1β after <t>lentiviral</t> downregulation of TG2 (TG2 knockdown (KD)) compared to scrambled knockdown (scrambled KD) ( f ). Fibril-like fibronectin deposition (indicated by arrows in c and e ) was morphological altered after treatment with ERW1041E and after lentiviral downregulation of TG2. Scale bar represents 20 μm
    Lentiviral Particles, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lentiviral particles/product/Santa Cruz Biotechnology
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lentiviral particles - by Bioz Stars, 2021-09
    90/100 stars
      Buy from Supplier

    99
    Santa Cruz Biotechnology control shrna lentiviral particles
    Endogenous TG2 affects the morphological appearance of fibronectin. Fibronectin (FN) aggregates on laminin (LAM) coated plastic were studied after removal of astrocytes that were allowed to produce extracellular matrix during 48 h of TNF-α+IL-1β treatment ( a , b ). Representative blots of four independent experiments are shown. Each bar represents the mean + standard error of the mean of signal intensities from blots of four separate experiments that were calculated as average percentage compared to control. Primary rat astrocytes were treated with TNF-α+IL-1β for 48 h plated on laminin coated plastic. Immunocytochemical double stainings showed immunoreactivity of fibronectin (FN, red) and TG2 (green) in astrocytes ( c , d , e , and f ). Astrocytes were co-incubated with TNF-α+IL-1β and the TG2-specific inhibitor ERW1041E (10 μM for 48 h) or DMSO ( d ) or treated with TNF-α+IL-1β after <t>lentiviral</t> downregulation of TG2 (TG2 knockdown (KD)) compared to scrambled knockdown (scrambled KD) ( f ). Fibril-like fibronectin deposition (indicated by arrows in c and e ) was morphological altered after treatment with ERW1041E and after lentiviral downregulation of TG2. Scale bar represents 20 μm
    Control Shrna Lentiviral Particles, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control shrna lentiviral particles/product/Santa Cruz Biotechnology
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    control shrna lentiviral particles - by Bioz Stars, 2021-09
    99/100 stars
      Buy from Supplier

    92
    Santa Cruz Biotechnology mcl 1
    The ( <t>MCL-1</t> + BFL-1 )/ BCL-2 represents the most significant linear correlation for sensitivity to ABT-737 in SCLC. SCLC cell lines with a wide range of response (resistant, intermediate, and sensitive) to ABT-737 were plotted against: (A) ( MCL-1 + BFL-1
    Mcl 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mcl 1/product/Santa Cruz Biotechnology
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mcl 1 - by Bioz Stars, 2021-09
    92/100 stars
      Buy from Supplier

    Image Search Results


    Upregulation of UCP2 by TRPV1 activation attenuates hyperglycemia-induced ROS production in ECs. The increased metabolism of glucose due to intracellular hyperglycemia leads to the overproduction of NADH, a critical component of the superoxide-generating mechanism in endothelial cells. Upregulation of mitochondrial UCP2 in response to elevated superoxide levels plays an active role in the feedback regulation of reactive oxygen species production that is associated with chronic oxidative stress. Activation of the endothelial TRPV1 channel in endothelial cells by dietary capsaicin mediates the phosphorylation of PKA and upregulates UCP2, thus inhibiting the activity of NADPH and decreasing ROS production in ECs.

    Journal: Cardiovascular Diabetology

    Article Title: TRPV1-mediated UCP2 upregulation ameliorates hyperglycemia-induced endothelial dysfunction

    doi: 10.1186/1475-2840-12-69

    Figure Lengend Snippet: Upregulation of UCP2 by TRPV1 activation attenuates hyperglycemia-induced ROS production in ECs. The increased metabolism of glucose due to intracellular hyperglycemia leads to the overproduction of NADH, a critical component of the superoxide-generating mechanism in endothelial cells. Upregulation of mitochondrial UCP2 in response to elevated superoxide levels plays an active role in the feedback regulation of reactive oxygen species production that is associated with chronic oxidative stress. Activation of the endothelial TRPV1 channel in endothelial cells by dietary capsaicin mediates the phosphorylation of PKA and upregulates UCP2, thus inhibiting the activity of NADPH and decreasing ROS production in ECs.

    Article Snippet: First, upregulation of UCP2 by capsaicin reduced the production of ROS and increased the levels of NO in cultured endothelial cells through a TRPV1 activation-mediated PKA pathway.

    Techniques: Activation Assay, Activity Assay

    The effect of TRPV1 activation on the production of ROS and NO through the PKA/UCP2 pathway. A and B : Representative western blot images ( A ) and summary data ( B ) showing P22 phox protein level in endothelial cells cultured with normal-glucose (NG, glucose 5.5 mmol/L), high-glucose (HG, glucose 30 mmol/L), HG+capsaicin (HG+Cap, Cap 1 μmol/L), HG+Cap+5’-iodo-resiniferatoxin (HG+Cap+iRTX, iRTX 1 μmol/L), HG+Cap+KT5720 (2 μmol/L), HG+Cap+Genipin (10 μmol/L). ## P

    Journal: Cardiovascular Diabetology

    Article Title: TRPV1-mediated UCP2 upregulation ameliorates hyperglycemia-induced endothelial dysfunction

    doi: 10.1186/1475-2840-12-69

    Figure Lengend Snippet: The effect of TRPV1 activation on the production of ROS and NO through the PKA/UCP2 pathway. A and B : Representative western blot images ( A ) and summary data ( B ) showing P22 phox protein level in endothelial cells cultured with normal-glucose (NG, glucose 5.5 mmol/L), high-glucose (HG, glucose 30 mmol/L), HG+capsaicin (HG+Cap, Cap 1 μmol/L), HG+Cap+5’-iodo-resiniferatoxin (HG+Cap+iRTX, iRTX 1 μmol/L), HG+Cap+KT5720 (2 μmol/L), HG+Cap+Genipin (10 μmol/L). ## P

    Article Snippet: First, upregulation of UCP2 by capsaicin reduced the production of ROS and increased the levels of NO in cultured endothelial cells through a TRPV1 activation-mediated PKA pathway.

    Techniques: Activation Assay, Western Blot, Cell Culture

    TRPV1 activation ameliorates high-glucose-induced endothelial dysfunction in a UCP2-dependent manner. A : Representative immunofluorescence images showing the co-expression of TRPV1, PKA and UCP2 in the aortas from wild type mice, particularly in the endothelium (Bar denotes 50 μm). B and C : Acetylcholine (1 nmol/L to 10 μmol/L)-induced endothelium-dependent relaxation of isolated aortic artery rings from wild type and TRPV1 -/- mice, pre-incubated with normal-glucose for 12 hours (NG, glucose 5.5 mmol/L), high-glucose (HG, glucose 30 mmol/L), HG+capsaicin (HG+Cap, Cap 1 μmol/L), HG+Cap+KT5720 (2 μmol/L); **P

    Journal: Cardiovascular Diabetology

    Article Title: TRPV1-mediated UCP2 upregulation ameliorates hyperglycemia-induced endothelial dysfunction

    doi: 10.1186/1475-2840-12-69

    Figure Lengend Snippet: TRPV1 activation ameliorates high-glucose-induced endothelial dysfunction in a UCP2-dependent manner. A : Representative immunofluorescence images showing the co-expression of TRPV1, PKA and UCP2 in the aortas from wild type mice, particularly in the endothelium (Bar denotes 50 μm). B and C : Acetylcholine (1 nmol/L to 10 μmol/L)-induced endothelium-dependent relaxation of isolated aortic artery rings from wild type and TRPV1 -/- mice, pre-incubated with normal-glucose for 12 hours (NG, glucose 5.5 mmol/L), high-glucose (HG, glucose 30 mmol/L), HG+capsaicin (HG+Cap, Cap 1 μmol/L), HG+Cap+KT5720 (2 μmol/L); **P

    Article Snippet: First, upregulation of UCP2 by capsaicin reduced the production of ROS and increased the levels of NO in cultured endothelial cells through a TRPV1 activation-mediated PKA pathway.

    Techniques: Activation Assay, Immunofluorescence, Expressing, Mouse Assay, Isolation, Incubation

    TRPV1 activation by dietary capsaicin promotes endothelial PKA phosphorylation and increases UCP2 levels in diabetic mice. Representative protein expression of TRPV1 ( A and B ), p-PKA/PKA ( C and D ) and UCP2 ( E ) levels in aorta or mesenteric arteries from db/db mice treated with normal diet (db/db Cont) or normal diet plus 0.01% capsaicin (db/db Cap) and the lean littermate control C57BL/KsJ mice treated with normal diet (WT Cont). Data are mean ± SEM. Each n = 3. ## P

    Journal: Cardiovascular Diabetology

    Article Title: TRPV1-mediated UCP2 upregulation ameliorates hyperglycemia-induced endothelial dysfunction

    doi: 10.1186/1475-2840-12-69

    Figure Lengend Snippet: TRPV1 activation by dietary capsaicin promotes endothelial PKA phosphorylation and increases UCP2 levels in diabetic mice. Representative protein expression of TRPV1 ( A and B ), p-PKA/PKA ( C and D ) and UCP2 ( E ) levels in aorta or mesenteric arteries from db/db mice treated with normal diet (db/db Cont) or normal diet plus 0.01% capsaicin (db/db Cap) and the lean littermate control C57BL/KsJ mice treated with normal diet (WT Cont). Data are mean ± SEM. Each n = 3. ## P

    Article Snippet: First, upregulation of UCP2 by capsaicin reduced the production of ROS and increased the levels of NO in cultured endothelial cells through a TRPV1 activation-mediated PKA pathway.

    Techniques: Activation Assay, Mouse Assay, Expressing

    Activation of TRPV1 up-regulates UCP2 through PKA phosphorylation. A , B and C : Representative western blot images showing protein expressions of TRPV1 ( A ), p-PKA/PKA ( B ) and UCP2 ( C ) in endothelial cells cultured with normal-glucose (NG, glucose 5.5 mmol/L), high-glucose (HG, glucose 30 mmol/L), HG+capsaicin (HG+Cap, Cap 1 μmol/L), HG+Cap+5’-iodo-resiniferatoxin (HG+Cap+iRTX, iRTX 1 μmol/L), HG+Cap+KT5720 (2 μmol/L) or HG+Cap+Genipin (10 μmol/L); #P

    Journal: Cardiovascular Diabetology

    Article Title: TRPV1-mediated UCP2 upregulation ameliorates hyperglycemia-induced endothelial dysfunction

    doi: 10.1186/1475-2840-12-69

    Figure Lengend Snippet: Activation of TRPV1 up-regulates UCP2 through PKA phosphorylation. A , B and C : Representative western blot images showing protein expressions of TRPV1 ( A ), p-PKA/PKA ( B ) and UCP2 ( C ) in endothelial cells cultured with normal-glucose (NG, glucose 5.5 mmol/L), high-glucose (HG, glucose 30 mmol/L), HG+capsaicin (HG+Cap, Cap 1 μmol/L), HG+Cap+5’-iodo-resiniferatoxin (HG+Cap+iRTX, iRTX 1 μmol/L), HG+Cap+KT5720 (2 μmol/L) or HG+Cap+Genipin (10 μmol/L); #P

    Article Snippet: First, upregulation of UCP2 by capsaicin reduced the production of ROS and increased the levels of NO in cultured endothelial cells through a TRPV1 activation-mediated PKA pathway.

    Techniques: Activation Assay, Western Blot, Cell Culture

    Endogenous TG2 affects the morphological appearance of fibronectin. Fibronectin (FN) aggregates on laminin (LAM) coated plastic were studied after removal of astrocytes that were allowed to produce extracellular matrix during 48 h of TNF-α+IL-1β treatment ( a , b ). Representative blots of four independent experiments are shown. Each bar represents the mean + standard error of the mean of signal intensities from blots of four separate experiments that were calculated as average percentage compared to control. Primary rat astrocytes were treated with TNF-α+IL-1β for 48 h plated on laminin coated plastic. Immunocytochemical double stainings showed immunoreactivity of fibronectin (FN, red) and TG2 (green) in astrocytes ( c , d , e , and f ). Astrocytes were co-incubated with TNF-α+IL-1β and the TG2-specific inhibitor ERW1041E (10 μM for 48 h) or DMSO ( d ) or treated with TNF-α+IL-1β after lentiviral downregulation of TG2 (TG2 knockdown (KD)) compared to scrambled knockdown (scrambled KD) ( f ). Fibril-like fibronectin deposition (indicated by arrows in c and e ) was morphological altered after treatment with ERW1041E and after lentiviral downregulation of TG2. Scale bar represents 20 μm

    Journal: Journal of Neuroinflammation

    Article Title: Tissue transglutaminase in astrocytes is enhanced by inflammatory mediators and is involved in the formation of fibronectin fibril-like structures

    doi: 10.1186/s12974-017-1031-2

    Figure Lengend Snippet: Endogenous TG2 affects the morphological appearance of fibronectin. Fibronectin (FN) aggregates on laminin (LAM) coated plastic were studied after removal of astrocytes that were allowed to produce extracellular matrix during 48 h of TNF-α+IL-1β treatment ( a , b ). Representative blots of four independent experiments are shown. Each bar represents the mean + standard error of the mean of signal intensities from blots of four separate experiments that were calculated as average percentage compared to control. Primary rat astrocytes were treated with TNF-α+IL-1β for 48 h plated on laminin coated plastic. Immunocytochemical double stainings showed immunoreactivity of fibronectin (FN, red) and TG2 (green) in astrocytes ( c , d , e , and f ). Astrocytes were co-incubated with TNF-α+IL-1β and the TG2-specific inhibitor ERW1041E (10 μM for 48 h) or DMSO ( d ) or treated with TNF-α+IL-1β after lentiviral downregulation of TG2 (TG2 knockdown (KD)) compared to scrambled knockdown (scrambled KD) ( f ). Fibril-like fibronectin deposition (indicated by arrows in c and e ) was morphological altered after treatment with ERW1041E and after lentiviral downregulation of TG2. Scale bar represents 20 μm

    Article Snippet: The following day, 0.2 × 106 infectious units of virus (IFU) of lentiviral particles (Santa Cruz, sc-270266-V for rat TG2 shRNA, or sc-108080 for control scrambled shRNA) were added to the cells.

    Techniques: Laser Capture Microdissection, Incubation

    The ( MCL-1 + BFL-1 )/ BCL-2 represents the most significant linear correlation for sensitivity to ABT-737 in SCLC. SCLC cell lines with a wide range of response (resistant, intermediate, and sensitive) to ABT-737 were plotted against: (A) ( MCL-1 + BFL-1

    Journal: Blood

    Article Title: An antiapoptotic BCL-2 family expression index predicts the response of chronic lymphocytic leukemia to ABT-737

    doi: 10.1182/blood-2011-03-340364

    Figure Lengend Snippet: The ( MCL-1 + BFL-1 )/ BCL-2 represents the most significant linear correlation for sensitivity to ABT-737 in SCLC. SCLC cell lines with a wide range of response (resistant, intermediate, and sensitive) to ABT-737 were plotted against: (A) ( MCL-1 + BFL-1

    Article Snippet: Lentiviral particles targeting 3 different regions of MCL-1 (sc-35877-V), and BFL-1 (sc-37285-V), and the scrambled shRNA (sc-108080) were obtained from Santa Cruz Biotechnology.

    Techniques:

    Decreased Mcl-1 and/or Bfl-1 levels enhance ABT-737 response in resistant CLL cell lines

    Journal: Blood

    Article Title: An antiapoptotic BCL-2 family expression index predicts the response of chronic lymphocytic leukemia to ABT-737

    doi: 10.1182/blood-2011-03-340364

    Figure Lengend Snippet: Decreased Mcl-1 and/or Bfl-1 levels enhance ABT-737 response in resistant CLL cell lines

    Article Snippet: Lentiviral particles targeting 3 different regions of MCL-1 (sc-35877-V), and BFL-1 (sc-37285-V), and the scrambled shRNA (sc-108080) were obtained from Santa Cruz Biotechnology.

    Techniques:

    ( MCL-1 + BFL-1 )/ BCL-2 provides the most significant linear correlation for sensitivity to ABT-737. Cell viability after ABT-737 treatment was plotted against: (A) ( MCL-1 + BFL-1 )/ BCL-2 , (B) MCL-1 / BCL-2 , (C) BFL-1 / BCL-2 , (D) BCL-B / BCL-2 , (E) ( MCL-1 +

    Journal: Blood

    Article Title: An antiapoptotic BCL-2 family expression index predicts the response of chronic lymphocytic leukemia to ABT-737

    doi: 10.1182/blood-2011-03-340364

    Figure Lengend Snippet: ( MCL-1 + BFL-1 )/ BCL-2 provides the most significant linear correlation for sensitivity to ABT-737. Cell viability after ABT-737 treatment was plotted against: (A) ( MCL-1 + BFL-1 )/ BCL-2 , (B) MCL-1 / BCL-2 , (C) BFL-1 / BCL-2 , (D) BCL-B / BCL-2 , (E) ( MCL-1 +

    Article Snippet: Lentiviral particles targeting 3 different regions of MCL-1 (sc-35877-V), and BFL-1 (sc-37285-V), and the scrambled shRNA (sc-108080) were obtained from Santa Cruz Biotechnology.

    Techniques:

    Flavopiridol and shRNA decrease MCL-1 and BFL-1 levels and increase the response to ABT-737 in resistant CLL cell lines. (A) Mec-1, Mec-2, and PTA CLL cells were treated with flavopiridol for 4 hours, and then BCL-2 , MCL-1 , and BFL-1 levels were assessed

    Journal: Blood

    Article Title: An antiapoptotic BCL-2 family expression index predicts the response of chronic lymphocytic leukemia to ABT-737

    doi: 10.1182/blood-2011-03-340364

    Figure Lengend Snippet: Flavopiridol and shRNA decrease MCL-1 and BFL-1 levels and increase the response to ABT-737 in resistant CLL cell lines. (A) Mec-1, Mec-2, and PTA CLL cells were treated with flavopiridol for 4 hours, and then BCL-2 , MCL-1 , and BFL-1 levels were assessed

    Article Snippet: Lentiviral particles targeting 3 different regions of MCL-1 (sc-35877-V), and BFL-1 (sc-37285-V), and the scrambled shRNA (sc-108080) were obtained from Santa Cruz Biotechnology.

    Techniques: shRNA