Journal: Antimicrobial Agents and Chemotherapy
Article Title: Molecular Epidemiology, Sequence Types, and Plasmid Analyses of KPC-Producing Klebsiella pneumoniae Strains in Israel ▿
Figure Lengend Snippet: A comparison of bla KPC-2 -carrying plasmids originated from two K. pneumoniae clones and two E. coli clones isolated in the same time period. Plasmid restriction analysis of transformants carrying these plasmids showed identity between the K. pneumoniae plasmids (K) and the E. coli plasmids (E). Plasmids from both organisms were digested with BglII (lanes 2 to 5), EcoRV (lanes 6 to 9), and SmaI (lanes 10 to 13) prior to electrophoresis. GeneRuler 1-kb DNA ladder (Fermentas Life Sciences), lane 1 (M); E. coli 386, lanes 2, 6, and 10; K. pneumoniae 523 PFGE type R, lanes 3, 7, and 11; E. coli 547, lanes 4, 8, and 12); K. pneumoniae 531, PFGE type, lanes 5, 9, and 13.
Article Snippet: Plasmids isolated from clinical isolates of K. pneumoniae and E. coli strains and their transformants were digested with different restriction endonucleases, such as BglII, SmaI, and EcoRV (New England Biolabs, Boston, MA), and their restriction patterns were compared.
Techniques: Clone Assay, Isolation, Plasmid Preparation, Electrophoresis