smai Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs smai
    Alterations of restriction enzyme accessibility in the juxtaposed region of Crabp1 promoter along the course of adipocyte differentiation. ( A ) Detection of nucleosome array. Chromatin of MNase partially digested nuclei was separated on agarose gels followed by Southern blot analysis (left panel) and EtBr staining (right panel). A probe used covering the TIS (HinfI-PvuII) was used for Southern blot analysis. ( B–F ) Nuclei (differentiation Days 0, 4, 8) were digested with the indicated restriction enzymes and the recovered chromatin DNAs were completely re-digested with ApaI ( B–D ) or <t>PstI</t> ( E and F ) (the relationship of specific restriction site and each individual nucleosome was depicted in panel G), followed by Southern blot analyses using probe 1 ( D ) or probe 2 (B–C and E–F). ( G ) Schematic description of restriction enzyme digestion, nucleosomes, and predicted fragments detected with probes on the Southern blots. Complete digestion with ApaI produced 1.3 kb, and complete digestion with PstI produced 1.65 kb fragments. The generated fragments by digestion with XhoI, PstI, <t>SmaI,</t> SpeI and ApaLI for the corresponding nucleosome are 0.93, 0.67, 0.86, 0.55 and 0.65 kb, respectively. Nucleosomes on this array were named N5 to N-1, from the 5′- to the 3′-ends of this chromatin segment. Experiments were performed for least three times.
    Smai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1712 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/smai/product/New England Biolabs
    Average 99 stars, based on 1712 article reviews
    Price from $9.99 to $1999.99
    smai - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    93
    Thermo Fisher smai sites
    Linear cassette(s) building from vector <t>pMM-25.</t> The “warehouse” vector pMM-25 was <t>SmaI-cut,</t> and the 1.3-Kb cassette purified by agarose gel electrophoresis using Nucleospin ExtractII columns (Machery Nagel, Bethlehem, USA). A further double enzymatic digestion (BglII/BanI) of the 1.3-Kb cassette generated three products (37, 539, and 729 bp), the last two of which were purified and used in the ligase reactions for building the cassettes
    Smai Sites, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/smai sites/product/Thermo Fisher
    Average 93 stars, based on 61 article reviews
    Price from $9.99 to $1999.99
    smai sites - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    99
    Millipore smai
    Linear cassette(s) building from vector <t>pMM-25.</t> The “warehouse” vector pMM-25 was <t>SmaI-cut,</t> and the 1.3-Kb cassette purified by agarose gel electrophoresis using Nucleospin ExtractII columns (Machery Nagel, Bethlehem, USA). A further double enzymatic digestion (BglII/BanI) of the 1.3-Kb cassette generated three products (37, 539, and 729 bp), the last two of which were purified and used in the ligase reactions for building the cassettes
    Smai, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/smai/product/Millipore
    Average 99 stars, based on 149 article reviews
    Price from $9.99 to $1999.99
    smai - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    94
    Thermo Fisher fastdigest smai
    Linear cassette(s) building from vector <t>pMM-25.</t> The “warehouse” vector pMM-25 was <t>SmaI-cut,</t> and the 1.3-Kb cassette purified by agarose gel electrophoresis using Nucleospin ExtractII columns (Machery Nagel, Bethlehem, USA). A further double enzymatic digestion (BglII/BanI) of the 1.3-Kb cassette generated three products (37, 539, and 729 bp), the last two of which were purified and used in the ligase reactions for building the cassettes
    Fastdigest Smai, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fastdigest smai/product/Thermo Fisher
    Average 94 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    fastdigest smai - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    95
    Thermo Fisher smai
    Map of the vector constructed for the expression of TrxIA-2 ic in E. coli . The pTrxFus vector was used to create a C-terminal fusion to E. coli thioredoxin. The IA-2 ic optimised sequence was inserted into the multiple cloning site of the expression vector and expressed as amino terminal fusion to the E. coli protein thioredoxin. This vector includes an enterokinase (EK) cleavage site that allows release of the native protein from Trx. To drive expression of thioredoxin fusions, pTrxFus uses the pL promoter from the λ bacteriophage and the AspA transcription terminator. Plasmid selection and maintenance is ensured by the presence of a beta-lactamase gene ( BLA ) that provides ampicillin resistance. <t>SmaI</t> and <t>XbaI</t> sites are indicated at the 3’ and 5’ ends of the IA-2 ic sequence. RBS: ribosome binding site
    Smai, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1026 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/smai/product/Thermo Fisher
    Average 95 stars, based on 1026 article reviews
    Price from $9.99 to $1999.99
    smai - by Bioz Stars, 2020-08
    95/100 stars
      Buy from Supplier

    Image Search Results


    Alterations of restriction enzyme accessibility in the juxtaposed region of Crabp1 promoter along the course of adipocyte differentiation. ( A ) Detection of nucleosome array. Chromatin of MNase partially digested nuclei was separated on agarose gels followed by Southern blot analysis (left panel) and EtBr staining (right panel). A probe used covering the TIS (HinfI-PvuII) was used for Southern blot analysis. ( B–F ) Nuclei (differentiation Days 0, 4, 8) were digested with the indicated restriction enzymes and the recovered chromatin DNAs were completely re-digested with ApaI ( B–D ) or PstI ( E and F ) (the relationship of specific restriction site and each individual nucleosome was depicted in panel G), followed by Southern blot analyses using probe 1 ( D ) or probe 2 (B–C and E–F). ( G ) Schematic description of restriction enzyme digestion, nucleosomes, and predicted fragments detected with probes on the Southern blots. Complete digestion with ApaI produced 1.3 kb, and complete digestion with PstI produced 1.65 kb fragments. The generated fragments by digestion with XhoI, PstI, SmaI, SpeI and ApaLI for the corresponding nucleosome are 0.93, 0.67, 0.86, 0.55 and 0.65 kb, respectively. Nucleosomes on this array were named N5 to N-1, from the 5′- to the 3′-ends of this chromatin segment. Experiments were performed for least three times.

    Journal: Nucleic Acids Research

    Article Title: RIP140 in thyroid hormone-repression and chromatin remodeling of Crabp1 gene during adipocyte differentiation

    doi: 10.1093/nar/gkp780

    Figure Lengend Snippet: Alterations of restriction enzyme accessibility in the juxtaposed region of Crabp1 promoter along the course of adipocyte differentiation. ( A ) Detection of nucleosome array. Chromatin of MNase partially digested nuclei was separated on agarose gels followed by Southern blot analysis (left panel) and EtBr staining (right panel). A probe used covering the TIS (HinfI-PvuII) was used for Southern blot analysis. ( B–F ) Nuclei (differentiation Days 0, 4, 8) were digested with the indicated restriction enzymes and the recovered chromatin DNAs were completely re-digested with ApaI ( B–D ) or PstI ( E and F ) (the relationship of specific restriction site and each individual nucleosome was depicted in panel G), followed by Southern blot analyses using probe 1 ( D ) or probe 2 (B–C and E–F). ( G ) Schematic description of restriction enzyme digestion, nucleosomes, and predicted fragments detected with probes on the Southern blots. Complete digestion with ApaI produced 1.3 kb, and complete digestion with PstI produced 1.65 kb fragments. The generated fragments by digestion with XhoI, PstI, SmaI, SpeI and ApaLI for the corresponding nucleosome are 0.93, 0.67, 0.86, 0.55 and 0.65 kb, respectively. Nucleosomes on this array were named N5 to N-1, from the 5′- to the 3′-ends of this chromatin segment. Experiments were performed for least three times.

    Article Snippet: Isolated nuclei from differentiating 3T3-L1 cells were digested with 100 U of PstI, XhoI, SmaI, SpeI and ApaLI (New England Biolabs) for 30 min.

    Techniques: Southern Blot, Staining, Produced, Generated

    (A) PFGE macrorestriction patterns of Streptococcus hyointestinalis strains restricted with SmaI. Lane 1, S. hyointestinalis DPC6484; lane 2, S. hyointestinalis LMG14579; lane 3, S. hyointestinalis LMG14581; lane 4, S. hyointestinalis LMG14582; lane 5, S. hyointestinalis LMG14583; lane 6, S. hyointestinalis LMG14585; lane 7, S. hyointestinalis LMG14586; lane 8, S. hyointestinalis LMG14587. (B) (i) PCR amplification of strains of S. hyointestinalis template DNA with nshT -, nshH -, nshF -, and nshR -specific primers. Lanes correspond to those in panel A. (ii) Comparison of antimicrobial activities of S. hyointesinalis strains DPC6484 (H), LMG14579, LMG14581, LMG14582, LMG14583, LMG14585, LMG14586, and LMG14587 against the indicator strain, L. delbrueckii subsp. bulgaricus LMG6901. Wells are labeled with H (for nisin H) and with the last two digits of the strain designation for the other strains.

    Journal: Applied and Environmental Microbiology

    Article Title: Nisin H Is a New Nisin Variant Produced by the Gut-Derived Strain Streptococcus hyointestinalis DPC6484

    doi: 10.1128/AEM.00212-15

    Figure Lengend Snippet: (A) PFGE macrorestriction patterns of Streptococcus hyointestinalis strains restricted with SmaI. Lane 1, S. hyointestinalis DPC6484; lane 2, S. hyointestinalis LMG14579; lane 3, S. hyointestinalis LMG14581; lane 4, S. hyointestinalis LMG14582; lane 5, S. hyointestinalis LMG14583; lane 6, S. hyointestinalis LMG14585; lane 7, S. hyointestinalis LMG14586; lane 8, S. hyointestinalis LMG14587. (B) (i) PCR amplification of strains of S. hyointestinalis template DNA with nshT -, nshH -, nshF -, and nshR -specific primers. Lanes correspond to those in panel A. (ii) Comparison of antimicrobial activities of S. hyointesinalis strains DPC6484 (H), LMG14579, LMG14581, LMG14582, LMG14583, LMG14585, LMG14586, and LMG14587 against the indicator strain, L. delbrueckii subsp. bulgaricus LMG6901. Wells are labeled with H (for nisin H) and with the last two digits of the strain designation for the other strains.

    Article Snippet: Molecular fingerprinting of S. hyointestinalis isolates was performed by pulsed-field gel electrophoresis (PFGE) as described by Simpson et al. (2002) ( ) using SmaI restriction endonucleases and DNA molecular weight markers (9.42 to 242.50 kb; New England BioLabs, Beverly, MA).

    Techniques: Polymerase Chain Reaction, Amplification, Labeling

    Linear cassette(s) building from vector pMM-25. The “warehouse” vector pMM-25 was SmaI-cut, and the 1.3-Kb cassette purified by agarose gel electrophoresis using Nucleospin ExtractII columns (Machery Nagel, Bethlehem, USA). A further double enzymatic digestion (BglII/BanI) of the 1.3-Kb cassette generated three products (37, 539, and 729 bp), the last two of which were purified and used in the ligase reactions for building the cassettes

    Journal: Radiation and Environmental Biophysics

    Article Title: Saccharomyces cerevisiae-based system for studying clustered DNA damages

    doi: 10.1007/s00411-010-0303-3

    Figure Lengend Snippet: Linear cassette(s) building from vector pMM-25. The “warehouse” vector pMM-25 was SmaI-cut, and the 1.3-Kb cassette purified by agarose gel electrophoresis using Nucleospin ExtractII columns (Machery Nagel, Bethlehem, USA). A further double enzymatic digestion (BglII/BanI) of the 1.3-Kb cassette generated three products (37, 539, and 729 bp), the last two of which were purified and used in the ligase reactions for building the cassettes

    Article Snippet: The plasmid pMM-25, was constructed by inserting the amplification product as a SmaI fragment (1,305 bp) into SmaI site in the MCS of plasmid pBluescript II SK ± (2,961 bp, Fermentas), thus generating a 4,266-bp vector (Supplementary Fig. 2B).

    Techniques: Plasmid Preparation, Purification, Agarose Gel Electrophoresis, Generated

    PFGE fingerprints of 18 strains from patient 3 in chronological order. The patient harbors four clones: clone 4 (lanes 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, and 16), clone 6 (lanes 14 and 18), clone 7 (lane 15), and clone 8 (lane 17). Clones 6 and 8 showed the same biotype (III). (A) SmaI PFGE fingerprints; (B) Bsp120 I (ApaI) PFGE fingerprints. M is the molecular size marker (PFGE marker). PFGE patterns of the same strains with Bsp120 I (ApaI) are shown in the second photo.

    Journal: Journal of Clinical Microbiology

    Article Title: Dynamics of Long-Term Colonization of Respiratory Tract by Haemophilus influenzae in Cystic Fibrosis Patients Shows a Marked Increase in Hypermutable Strains

    doi: 10.1128/JCM.42.4.1450-1459.2004

    Figure Lengend Snippet: PFGE fingerprints of 18 strains from patient 3 in chronological order. The patient harbors four clones: clone 4 (lanes 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, and 16), clone 6 (lanes 14 and 18), clone 7 (lane 15), and clone 8 (lane 17). Clones 6 and 8 showed the same biotype (III). (A) SmaI PFGE fingerprints; (B) Bsp120 I (ApaI) PFGE fingerprints. M is the molecular size marker (PFGE marker). PFGE patterns of the same strains with Bsp120 I (ApaI) are shown in the second photo.

    Article Snippet: The restriction endonucleases SmaI and Bsp120 I (ApaI) (MBI Fermentas, Vilnius, Lithuania) were used at the manufacturer's suggested temperature.

    Techniques: Clone Assay, Marker

    PFGE fingerprints of 15 strains from patient 1 in chronological order. The patient harbors three patterns: pattern 1A (lanes 1, 3, 5, 6, 10, 11, 12, 13, 14, and 15), pattern 1B (lanes 2, 4, 7, and 8), and pattern 2 (lane 9). Pattern 1B strains showed the same biotype (VI) as six strains (lanes 1, 3, 5, 6, 11, and 15) of pattern 1A and one strain of pattern 2 (lane 9). (A) SmaI PFGE fingerprints; (B) Bsp120 I (ApaI) PFGE fingerprints. M is the molecular size marker (PFGE marker).

    Journal: Journal of Clinical Microbiology

    Article Title: Dynamics of Long-Term Colonization of Respiratory Tract by Haemophilus influenzae in Cystic Fibrosis Patients Shows a Marked Increase in Hypermutable Strains

    doi: 10.1128/JCM.42.4.1450-1459.2004

    Figure Lengend Snippet: PFGE fingerprints of 15 strains from patient 1 in chronological order. The patient harbors three patterns: pattern 1A (lanes 1, 3, 5, 6, 10, 11, 12, 13, 14, and 15), pattern 1B (lanes 2, 4, 7, and 8), and pattern 2 (lane 9). Pattern 1B strains showed the same biotype (VI) as six strains (lanes 1, 3, 5, 6, 11, and 15) of pattern 1A and one strain of pattern 2 (lane 9). (A) SmaI PFGE fingerprints; (B) Bsp120 I (ApaI) PFGE fingerprints. M is the molecular size marker (PFGE marker).

    Article Snippet: The restriction endonucleases SmaI and Bsp120 I (ApaI) (MBI Fermentas, Vilnius, Lithuania) were used at the manufacturer's suggested temperature.

    Techniques: Marker

    PFGE fingerprints of 27 strains from patient 28 in chronological order. The patient harbors five clones: clone 101 (lanes 1, 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 19, 20, 21, 24, 25, 26, and 27), clone 102 (lane 5), clone 103 (lane 18), clone 104 (lane 22), and clone 105 (lane 23). Three strains of clones 102 (lane 5), 103 (lane 18), and 104 (lane 22) and 20 strains (lanes 1, 2, 3, 4, 6, 7, 8, 9, 11, 12, 13, 14, 16, 17, 19, 20, 21, 24, 25, and 26) of clone 101 showed the same biotype (I). Clone 105 and two isolates of clone 101 (lanes 10 and 27) showed the same biotype (II). (A) SmaI PFGE fingerprints; (B) Bsp120 I (ApaI) PFGE fingerprints. M is the molecular size marker (PFGE marker).

    Journal: Journal of Clinical Microbiology

    Article Title: Dynamics of Long-Term Colonization of Respiratory Tract by Haemophilus influenzae in Cystic Fibrosis Patients Shows a Marked Increase in Hypermutable Strains

    doi: 10.1128/JCM.42.4.1450-1459.2004

    Figure Lengend Snippet: PFGE fingerprints of 27 strains from patient 28 in chronological order. The patient harbors five clones: clone 101 (lanes 1, 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 19, 20, 21, 24, 25, 26, and 27), clone 102 (lane 5), clone 103 (lane 18), clone 104 (lane 22), and clone 105 (lane 23). Three strains of clones 102 (lane 5), 103 (lane 18), and 104 (lane 22) and 20 strains (lanes 1, 2, 3, 4, 6, 7, 8, 9, 11, 12, 13, 14, 16, 17, 19, 20, 21, 24, 25, and 26) of clone 101 showed the same biotype (I). Clone 105 and two isolates of clone 101 (lanes 10 and 27) showed the same biotype (II). (A) SmaI PFGE fingerprints; (B) Bsp120 I (ApaI) PFGE fingerprints. M is the molecular size marker (PFGE marker).

    Article Snippet: The restriction endonucleases SmaI and Bsp120 I (ApaI) (MBI Fermentas, Vilnius, Lithuania) were used at the manufacturer's suggested temperature.

    Techniques: Clone Assay, Marker

    Map of the vector constructed for the expression of TrxIA-2 ic in E. coli . The pTrxFus vector was used to create a C-terminal fusion to E. coli thioredoxin. The IA-2 ic optimised sequence was inserted into the multiple cloning site of the expression vector and expressed as amino terminal fusion to the E. coli protein thioredoxin. This vector includes an enterokinase (EK) cleavage site that allows release of the native protein from Trx. To drive expression of thioredoxin fusions, pTrxFus uses the pL promoter from the λ bacteriophage and the AspA transcription terminator. Plasmid selection and maintenance is ensured by the presence of a beta-lactamase gene ( BLA ) that provides ampicillin resistance. SmaI and XbaI sites are indicated at the 3’ and 5’ ends of the IA-2 ic sequence. RBS: ribosome binding site

    Journal: BMC Biotechnology

    Article Title: Novel prokaryotic expression of thioredoxin-fused insulinoma associated protein tyrosine phosphatase 2 (IA-2), its characterization and immunodiagnostic application

    doi: 10.1186/s12896-016-0309-2

    Figure Lengend Snippet: Map of the vector constructed for the expression of TrxIA-2 ic in E. coli . The pTrxFus vector was used to create a C-terminal fusion to E. coli thioredoxin. The IA-2 ic optimised sequence was inserted into the multiple cloning site of the expression vector and expressed as amino terminal fusion to the E. coli protein thioredoxin. This vector includes an enterokinase (EK) cleavage site that allows release of the native protein from Trx. To drive expression of thioredoxin fusions, pTrxFus uses the pL promoter from the λ bacteriophage and the AspA transcription terminator. Plasmid selection and maintenance is ensured by the presence of a beta-lactamase gene ( BLA ) that provides ampicillin resistance. SmaI and XbaI sites are indicated at the 3’ and 5’ ends of the IA-2 ic sequence. RBS: ribosome binding site

    Article Snippet: After vector propagation and purification with QIAprep spin Miniprep Kit (QIAGEN, Hilden, Germany), the IA-2ic construct was released with SmaI and XbaI and ligated to the pTrxFus linearized vector (Invitrogen, Carlsbad, CA, USA) to yield pTrxIA-2ic (Fig. ).

    Techniques: Plasmid Preparation, Construct, Expressing, IA, Sequencing, Clone Assay, Selection, Binding Assay