singleplex Search Results


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  • 93
    New England Biolabs nebnext singleplex
    Nebnext Singleplex, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext singleplex/product/New England Biolabs
    Average 93 stars, based on 2 article reviews
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    nebnext singleplex - by Bioz Stars, 2020-02
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    90
    Thermo Fisher quantigene assay protocol
    Validation of <t>QuantiGene</t> probes sets shows good linearity with high signal to noise ratio for several mRNA targets in different tissues. Various mRNAs were quantified from different tissue lysates. (A) Human Huntingtin ( HTT ) quantified from YAC128 mouse
    Quantigene Assay Protocol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantigene assay protocol/product/Thermo Fisher
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    89
    Bio-Rad singleplex assays
    MIF is highly expressed in gastric and colon tumors and human tissue-derived fibroblasts. MIF mRNA levels measured by qRT PCR are increased in A) human gastric tumor samples and at higher levels in tissues from patients with nodal involvement, B) human colon tumor samples and at higher levels in tissues from patients with nodal involvement, and C) in the supernatants tumor derived gastric and colon fibroblasts compared to matched normal as measured by Luminex <t>singleplex</t> assay. N = 8 for C and the mean ± standard error are shown as the results of duplicated in multiple experiments. * p
    Singleplex Assays, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 89/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    SPSS Inc singleplex assays
    MIF is highly expressed in gastric and colon tumors and human tissue-derived fibroblasts. MIF mRNA levels measured by qRT PCR are increased in A) human gastric tumor samples and at higher levels in tissues from patients with nodal involvement, B) human colon tumor samples and at higher levels in tissues from patients with nodal involvement, and C) in the supernatants tumor derived gastric and colon fibroblasts compared to matched normal as measured by Luminex <t>singleplex</t> assay. N = 8 for C and the mean ± standard error are shown as the results of duplicated in multiple experiments. * p
    Singleplex Assays, supplied by SPSS Inc, used in various techniques. Bioz Stars score: 78/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/singleplex assays/product/SPSS Inc
    Average 78 stars, based on 2 article reviews
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    75
    Meso Scale Diagnostics LLC singleplex kit
    MIF is highly expressed in gastric and colon tumors and human tissue-derived fibroblasts. MIF mRNA levels measured by qRT PCR are increased in A) human gastric tumor samples and at higher levels in tissues from patients with nodal involvement, B) human colon tumor samples and at higher levels in tissues from patients with nodal involvement, and C) in the supernatants tumor derived gastric and colon fibroblasts compared to matched normal as measured by Luminex <t>singleplex</t> assay. N = 8 for C and the mean ± standard error are shown as the results of duplicated in multiple experiments. * p
    Singleplex Kit, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 75/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/singleplex kit/product/Meso Scale Diagnostics LLC
    Average 75 stars, based on 3 article reviews
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    81
    BioFire Diagnostics singleplex pcr
    MIF is highly expressed in gastric and colon tumors and human tissue-derived fibroblasts. MIF mRNA levels measured by qRT PCR are increased in A) human gastric tumor samples and at higher levels in tissues from patients with nodal involvement, B) human colon tumor samples and at higher levels in tissues from patients with nodal involvement, and C) in the supernatants tumor derived gastric and colon fibroblasts compared to matched normal as measured by Luminex <t>singleplex</t> assay. N = 8 for C and the mean ± standard error are shown as the results of duplicated in multiple experiments. * p
    Singleplex Pcr, supplied by BioFire Diagnostics, used in various techniques. Bioz Stars score: 81/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 81 stars, based on 5 article reviews
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    94
    Thermo Fisher singleplex
    The shift from extract-based to molecule-based allergy diagnosis. For molecule-based <t>singleplex</t> approaches, the number of tests to be performed can be very high. Allergen microarrays offer the advantage of testing a large panel of molecules in one single test.
    Singleplex, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Millipore singleplex cytokine panel
    The shift from extract-based to molecule-based allergy diagnosis. For molecule-based <t>singleplex</t> approaches, the number of tests to be performed can be very high. Allergen microarrays offer the advantage of testing a large panel of molecules in one single test.
    Singleplex Cytokine Panel, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    R&D Systems singleplex immunoassays
    The shift from extract-based to molecule-based allergy diagnosis. For molecule-based <t>singleplex</t> approaches, the number of tests to be performed can be very high. Allergen microarrays offer the advantage of testing a large panel of molecules in one single test.
    Singleplex Immunoassays, supplied by R&D Systems, used in various techniques. Bioz Stars score: 79/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Thermo Fisher cytokine singleplex assays
    The shift from extract-based to molecule-based allergy diagnosis. For molecule-based <t>singleplex</t> approaches, the number of tests to be performed can be very high. Allergen microarrays offer the advantage of testing a large panel of molecules in one single test.
    Cytokine Singleplex Assays, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Assay Designs Inc singleplex multibead kit
    The shift from extract-based to molecule-based allergy diagnosis. For molecule-based <t>singleplex</t> approaches, the number of tests to be performed can be very high. Allergen microarrays offer the advantage of testing a large panel of molecules in one single test.
    Singleplex Multibead Kit, supplied by Assay Designs Inc, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Millipore singleplex immunoassay
    The shift from extract-based to molecule-based allergy diagnosis. For molecule-based <t>singleplex</t> approaches, the number of tests to be performed can be very high. Allergen microarrays offer the advantage of testing a large panel of molecules in one single test.
    Singleplex Immunoassay, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Luminex singleplex
    Receiver-Operating Characteristic (ROC) analysis for the multiplex RT-PCR-Luminex assay versus <t>singleplex</t> qRT-PCR as the gold standard.
    Singleplex, supplied by Luminex, used in various techniques. Bioz Stars score: 85/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    R&D Systems singleplex elisa
    Receiver-Operating Characteristic (ROC) analysis for the multiplex RT-PCR-Luminex assay versus <t>singleplex</t> qRT-PCR as the gold standard.
    Singleplex Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 83/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Millipore millipore singleplex system
    Receiver-Operating Characteristic (ROC) analysis for the multiplex RT-PCR-Luminex assay versus <t>singleplex</t> qRT-PCR as the gold standard.
    Millipore Singleplex System, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher singleplex elisa
    Receiver-Operating Characteristic (ROC) analysis for the multiplex RT-PCR-Luminex assay versus <t>singleplex</t> qRT-PCR as the gold standard.
    Singleplex Elisa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Meridian Life Science singleplex
    Receiver-Operating Characteristic (ROC) analysis for the multiplex RT-PCR-Luminex assay versus <t>singleplex</t> qRT-PCR as the gold standard.
    Singleplex, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 83/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Thermo Fisher singleplex qpcr
    Receiver-Operating Characteristic (ROC) analysis for the multiplex RT-PCR-Luminex assay versus <t>singleplex</t> qRT-PCR as the gold standard.
    Singleplex Qpcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher singleplex taqman assays
    Receiver-Operating Characteristic (ROC) analysis for the multiplex RT-PCR-Luminex assay versus <t>singleplex</t> qRT-PCR as the gold standard.
    Singleplex Taqman Assays, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Luminex singleplex liquid array system
    Receiver-Operating Characteristic (ROC) analysis for the multiplex RT-PCR-Luminex assay versus <t>singleplex</t> qRT-PCR as the gold standard.
    Singleplex Liquid Array System, supplied by Luminex, used in various techniques. Bioz Stars score: 86/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Millipore singleplex bone kit
    Receiver-Operating Characteristic (ROC) analysis for the multiplex RT-PCR-Luminex assay versus <t>singleplex</t> qRT-PCR as the gold standard.
    Singleplex Bone Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher singleplex bead kit
    Receiver-Operating Characteristic (ROC) analysis for the multiplex RT-PCR-Luminex assay versus <t>singleplex</t> qRT-PCR as the gold standard.
    Singleplex Bead Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Thermo Fisher il 13 human singleplex kit
    Receiver-Operating Characteristic (ROC) analysis for the multiplex RT-PCR-Luminex assay versus <t>singleplex</t> qRT-PCR as the gold standard.
    Il 13 Human Singleplex Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    fluidigm individual reaction chambers singleplex
    Receiver-Operating Characteristic (ROC) analysis for the multiplex RT-PCR-Luminex assay versus <t>singleplex</t> qRT-PCR as the gold standard.
    Individual Reaction Chambers Singleplex, supplied by fluidigm, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher singleplex control
    Receiver-Operating Characteristic (ROC) analysis for the multiplex RT-PCR-Luminex assay versus <t>singleplex</t> qRT-PCR as the gold standard.
    Singleplex Control, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    fluidigm singleplex assays
    Receiver-Operating Characteristic (ROC) analysis for the multiplex RT-PCR-Luminex assay versus <t>singleplex</t> qRT-PCR as the gold standard.
    Singleplex Assays, supplied by fluidigm, used in various techniques. Bioz Stars score: 77/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Thermo Fisher il 6 human singleplex kit
    Receiver-Operating Characteristic (ROC) analysis for the multiplex RT-PCR-Luminex assay versus <t>singleplex</t> qRT-PCR as the gold standard.
    Il 6 Human Singleplex Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    BioMed Diagnostics Inc singleplex approach
    Receiver-Operating Characteristic (ROC) analysis for the multiplex RT-PCR-Luminex assay versus <t>singleplex</t> qRT-PCR as the gold standard.
    Singleplex Approach, supplied by BioMed Diagnostics Inc, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    fluidigm singleplex amplification
    Pre-amplification multiplex results for bisulfite DNA samples. (A) A flow diagram outlining the different conditions examined with respect to multiplexability, pooling, and exonuclease treatment. (B) The results of different conditions after 15 cycles of multiplex pre-amplification. Forty-eight primer pairs were assessed for the multiplexability in a pre-amplification reaction. All samples had the same amount of input DNA. Lane 1 : A positive control involving <t>singleplex</t> PCR reaction of an individual primer pair, with the same DNA template amount as used in the pre-amplification. Lane 2 : Eight-plex pre-amplification reaction and ExoSAP-IT treatment of individual pre-amp reactions, followed by pooling and singleplex amplification (as illustrated in the upper panel of A). Lane 3 : Results of three different 8-plex reactions pooled together first then ExoSAP-IT treatment of the combined pool, for a total of 24 amplicons in the ExoSAP-IT treatment, followed by singleplex amplification of a primer pair (as illustrated in the lower panel of A). Lane 4 : Twenty-four-plex pre-amplification results. (C) The effect of ExoSAP-IT treatment on bisulfite libraries, as compared to gDNA amplicons. The arrow indicates where primers migrate on the gel. Lane 1 : Pool of 48 amplicons prior to barcoding PCR. Lane 2 : Sample library after barcoding PCR. Primers are visible at the bottom of the lane. Lane 3 : Sample library after ExoSAP-IT treatment at 37°C. Lane 4 : Sample library after ExoSAP-IT treatment at 37°C, followed by heat inactivation of the ExoSAP-IT at 80°C. Note the higher molecular weight smear in the methylation library, which is not observed with gDNA amplicons. Lane 5 : Sample library cycled at 37°C, followed by 80°C heat denaturation step, but with no ExoSAP-IT.
    Singleplex Amplification, supplied by fluidigm, used in various techniques. Bioz Stars score: 79/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher singleplex analysis
    Pre-amplification multiplex results for bisulfite DNA samples. (A) A flow diagram outlining the different conditions examined with respect to multiplexability, pooling, and exonuclease treatment. (B) The results of different conditions after 15 cycles of multiplex pre-amplification. Forty-eight primer pairs were assessed for the multiplexability in a pre-amplification reaction. All samples had the same amount of input DNA. Lane 1 : A positive control involving <t>singleplex</t> PCR reaction of an individual primer pair, with the same DNA template amount as used in the pre-amplification. Lane 2 : Eight-plex pre-amplification reaction and ExoSAP-IT treatment of individual pre-amp reactions, followed by pooling and singleplex amplification (as illustrated in the upper panel of A). Lane 3 : Results of three different 8-plex reactions pooled together first then ExoSAP-IT treatment of the combined pool, for a total of 24 amplicons in the ExoSAP-IT treatment, followed by singleplex amplification of a primer pair (as illustrated in the lower panel of A). Lane 4 : Twenty-four-plex pre-amplification results. (C) The effect of ExoSAP-IT treatment on bisulfite libraries, as compared to gDNA amplicons. The arrow indicates where primers migrate on the gel. Lane 1 : Pool of 48 amplicons prior to barcoding PCR. Lane 2 : Sample library after barcoding PCR. Primers are visible at the bottom of the lane. Lane 3 : Sample library after ExoSAP-IT treatment at 37°C. Lane 4 : Sample library after ExoSAP-IT treatment at 37°C, followed by heat inactivation of the ExoSAP-IT at 80°C. Note the higher molecular weight smear in the methylation library, which is not observed with gDNA amplicons. Lane 5 : Sample library cycled at 37°C, followed by 80°C heat denaturation step, but with no ExoSAP-IT.
    Singleplex Analysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Validation of QuantiGene probes sets shows good linearity with high signal to noise ratio for several mRNA targets in different tissues. Various mRNAs were quantified from different tissue lysates. (A) Human Huntingtin ( HTT ) quantified from YAC128 mouse

    Journal: Nucleic Acid Therapeutics

    Article Title: A High-Throughput Method for Direct Detection of Therapeutic Oligonucleotide-Induced Gene Silencing In Vivo

    doi: 10.1089/nat.2015.0578

    Figure Lengend Snippet: Validation of QuantiGene probes sets shows good linearity with high signal to noise ratio for several mRNA targets in different tissues. Various mRNAs were quantified from different tissue lysates. (A) Human Huntingtin ( HTT ) quantified from YAC128 mouse

    Article Snippet: The following day, the remainder of the QuantiGene assay protocol was performed (#QS0011; Affymetrix).

    Techniques:

    Combination of TissueLyser II with QuantiGene ® bDNA: A high-throughput method of direct mRNA detection from tissues biopsies. Top panel: Punch biopsies from consecutively sectioned coronal brain samples are collected. Biopsies are incubated at

    Journal: Nucleic Acid Therapeutics

    Article Title: A High-Throughput Method for Direct Detection of Therapeutic Oligonucleotide-Induced Gene Silencing In Vivo

    doi: 10.1089/nat.2015.0578

    Figure Lengend Snippet: Combination of TissueLyser II with QuantiGene ® bDNA: A high-throughput method of direct mRNA detection from tissues biopsies. Top panel: Punch biopsies from consecutively sectioned coronal brain samples are collected. Biopsies are incubated at

    Article Snippet: The following day, the remainder of the QuantiGene assay protocol was performed (#QS0011; Affymetrix).

    Techniques: High Throughput Screening Assay, Incubation

    MIF is highly expressed in gastric and colon tumors and human tissue-derived fibroblasts. MIF mRNA levels measured by qRT PCR are increased in A) human gastric tumor samples and at higher levels in tissues from patients with nodal involvement, B) human colon tumor samples and at higher levels in tissues from patients with nodal involvement, and C) in the supernatants tumor derived gastric and colon fibroblasts compared to matched normal as measured by Luminex singleplex assay. N = 8 for C and the mean ± standard error are shown as the results of duplicated in multiple experiments. * p

    Journal: PLoS ONE

    Article Title: Chronic Macrophage Migration Inhibitory Factor Exposure Induces Mesenchymal Epithelial Transition and Promotes Gastric and Colon Cancers

    doi: 10.1371/journal.pone.0098656

    Figure Lengend Snippet: MIF is highly expressed in gastric and colon tumors and human tissue-derived fibroblasts. MIF mRNA levels measured by qRT PCR are increased in A) human gastric tumor samples and at higher levels in tissues from patients with nodal involvement, B) human colon tumor samples and at higher levels in tissues from patients with nodal involvement, and C) in the supernatants tumor derived gastric and colon fibroblasts compared to matched normal as measured by Luminex singleplex assay. N = 8 for C and the mean ± standard error are shown as the results of duplicated in multiple experiments. * p

    Article Snippet: Luminex Bead Arrays MIF was measured in supernatants by singleplex bead assay (Biorad, Hurcules, CA) according to manufacturer’s instructions.

    Techniques: Derivative Assay, Quantitative RT-PCR, Luminex, Singleplex Assay

    The shift from extract-based to molecule-based allergy diagnosis. For molecule-based singleplex approaches, the number of tests to be performed can be very high. Allergen microarrays offer the advantage of testing a large panel of molecules in one single test.

    Journal: Yonsei Medical Journal

    Article Title: Molecular Approach to Allergy Diagnosis and Therapy

    doi: 10.3349/ymj.2014.55.4.839

    Figure Lengend Snippet: The shift from extract-based to molecule-based allergy diagnosis. For molecule-based singleplex approaches, the number of tests to be performed can be very high. Allergen microarrays offer the advantage of testing a large panel of molecules in one single test.

    Article Snippet: Presently, manufacturers offer molecular allergy diagnosis in singleplex (i.e., Thermo Fisher ImmunoCAP, Siemens ImmuLite, and HyCor HyTec) or multiplex [Thermo Fisher Immuno-Solid phase Allergen Chip (ISAC)] formats; both systems have their advantages and limitations.

    Techniques:

    Receiver-Operating Characteristic (ROC) analysis for the multiplex RT-PCR-Luminex assay versus singleplex qRT-PCR as the gold standard.

    Journal: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

    Article Title: Multiplex Reverse Transcription PCR Luminex Assay for Detection and Quantitation of Viral Agents of Gastroenteritis

    doi: 10.1016/j.jcv.2010.12.009

    Figure Lengend Snippet: Receiver-Operating Characteristic (ROC) analysis for the multiplex RT-PCR-Luminex assay versus singleplex qRT-PCR as the gold standard.

    Article Snippet: The limit of detection of the multiplex RT-PCR-Luminex assay correlated to ~102 –104 viral copies per gram of stool depending on the virus, whereas singleplex amplifications, either detected by Luminex or qRT-PCR usually detected 5–50 times lower levels.

    Techniques: Multiplex Assay, Reverse Transcription Polymerase Chain Reaction, Luminex, Quantitative RT-PCR

    Correlation between singleplex qRT-PCR results and the multiplex RT-PCR-Luminex assay on clinical specimens. Two hundred twenty nine diarrheal specimens from inpatients in Tanzania underwent nucleic acid extraction and testing by both singleplex qRT-PCR

    Journal: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

    Article Title: Multiplex Reverse Transcription PCR Luminex Assay for Detection and Quantitation of Viral Agents of Gastroenteritis

    doi: 10.1016/j.jcv.2010.12.009

    Figure Lengend Snippet: Correlation between singleplex qRT-PCR results and the multiplex RT-PCR-Luminex assay on clinical specimens. Two hundred twenty nine diarrheal specimens from inpatients in Tanzania underwent nucleic acid extraction and testing by both singleplex qRT-PCR

    Article Snippet: The limit of detection of the multiplex RT-PCR-Luminex assay correlated to ~102 –104 viral copies per gram of stool depending on the virus, whereas singleplex amplifications, either detected by Luminex or qRT-PCR usually detected 5–50 times lower levels.

    Techniques: Quantitative RT-PCR, Multiplex Assay, Reverse Transcription Polymerase Chain Reaction, Luminex

    Correlation between singleplex qRT-PCR Ct and the multiplex RT-PCR-Luminex assay MFI values on clinical specimens. Best fit lines and regression (R 2 ) were extrapolated.

    Journal: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

    Article Title: Multiplex Reverse Transcription PCR Luminex Assay for Detection and Quantitation of Viral Agents of Gastroenteritis

    doi: 10.1016/j.jcv.2010.12.009

    Figure Lengend Snippet: Correlation between singleplex qRT-PCR Ct and the multiplex RT-PCR-Luminex assay MFI values on clinical specimens. Best fit lines and regression (R 2 ) were extrapolated.

    Article Snippet: The limit of detection of the multiplex RT-PCR-Luminex assay correlated to ~102 –104 viral copies per gram of stool depending on the virus, whereas singleplex amplifications, either detected by Luminex or qRT-PCR usually detected 5–50 times lower levels.

    Techniques: Quantitative RT-PCR, Multiplex Assay, Reverse Transcription Polymerase Chain Reaction, Luminex

    Correlation between template copy number, detection by singleplex qRT-PCR (Ct), and multiplex RT-PCR-Luminex assay (median fluorescence intensity – negative control). A serial dilution of T7 RNA transcript for were prepared either alone in 1 mM

    Journal: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

    Article Title: Multiplex Reverse Transcription PCR Luminex Assay for Detection and Quantitation of Viral Agents of Gastroenteritis

    doi: 10.1016/j.jcv.2010.12.009

    Figure Lengend Snippet: Correlation between template copy number, detection by singleplex qRT-PCR (Ct), and multiplex RT-PCR-Luminex assay (median fluorescence intensity – negative control). A serial dilution of T7 RNA transcript for were prepared either alone in 1 mM

    Article Snippet: The limit of detection of the multiplex RT-PCR-Luminex assay correlated to ~102 –104 viral copies per gram of stool depending on the virus, whereas singleplex amplifications, either detected by Luminex or qRT-PCR usually detected 5–50 times lower levels.

    Techniques: Quantitative RT-PCR, Multiplex Assay, Reverse Transcription Polymerase Chain Reaction, Luminex, Fluorescence, Negative Control, Serial Dilution

    Validation of the multiplex Luminex assays by comparison with single antigen assays is shown. ( A ) This graph shows a comparison of single-antigen and multiplex Luminex immunoassays. Each of the 14 recombinant proteins was coupled to its assigned LumAvidin™ beads and incubated with a single patient serum at the indicated dilutions either individually (singleplex), or together with the other 13 antigens (multiplex). After a wash step, the secondary antibody, phycoerythrin-conjugated goat-anti-human immunoglobulin (IgG), was added and the fluorescence intensities were measured. Representative data for IsdB are shown. The interclass correlation (ICC) of singleplex versus multiplex assays was measured. ( B ) Data were generated for the other 13 antigens in single- and multiplex formats, and measured values for all eight dilutions of all 14 antigens were compiled. ( C ) The ELISA compared with Luminex immunoassay data are shown. The diagonal line in Illustrations B and C represents the formula of y = x.

    Journal: Clinical Orthopaedics and Related Research

    Article Title: A Diagnostic Serum Antibody Test for Patients With Staphylococcus aureus Osteomyelitis

    doi: 10.1007/s11999-015-4354-2

    Figure Lengend Snippet: Validation of the multiplex Luminex assays by comparison with single antigen assays is shown. ( A ) This graph shows a comparison of single-antigen and multiplex Luminex immunoassays. Each of the 14 recombinant proteins was coupled to its assigned LumAvidin™ beads and incubated with a single patient serum at the indicated dilutions either individually (singleplex), or together with the other 13 antigens (multiplex). After a wash step, the secondary antibody, phycoerythrin-conjugated goat-anti-human immunoglobulin (IgG), was added and the fluorescence intensities were measured. Representative data for IsdB are shown. The interclass correlation (ICC) of singleplex versus multiplex assays was measured. ( B ) Data were generated for the other 13 antigens in single- and multiplex formats, and measured values for all eight dilutions of all 14 antigens were compiled. ( C ) The ELISA compared with Luminex immunoassay data are shown. The diagonal line in Illustrations B and C represents the formula of y = x.

    Article Snippet: For validation of the multiplex assay of 14 antigens, titers from the multiplex analysis were compared with titers from singleplex Luminex assays for each antigen (Fig. ).

    Techniques: Multiplex Assay, Luminex, Recombinant, Incubation, Fluorescence, Immunocytochemistry, Generated, Enzyme-linked Immunosorbent Assay

    Pre-amplification multiplex results for bisulfite DNA samples. (A) A flow diagram outlining the different conditions examined with respect to multiplexability, pooling, and exonuclease treatment. (B) The results of different conditions after 15 cycles of multiplex pre-amplification. Forty-eight primer pairs were assessed for the multiplexability in a pre-amplification reaction. All samples had the same amount of input DNA. Lane 1 : A positive control involving singleplex PCR reaction of an individual primer pair, with the same DNA template amount as used in the pre-amplification. Lane 2 : Eight-plex pre-amplification reaction and ExoSAP-IT treatment of individual pre-amp reactions, followed by pooling and singleplex amplification (as illustrated in the upper panel of A). Lane 3 : Results of three different 8-plex reactions pooled together first then ExoSAP-IT treatment of the combined pool, for a total of 24 amplicons in the ExoSAP-IT treatment, followed by singleplex amplification of a primer pair (as illustrated in the lower panel of A). Lane 4 : Twenty-four-plex pre-amplification results. (C) The effect of ExoSAP-IT treatment on bisulfite libraries, as compared to gDNA amplicons. The arrow indicates where primers migrate on the gel. Lane 1 : Pool of 48 amplicons prior to barcoding PCR. Lane 2 : Sample library after barcoding PCR. Primers are visible at the bottom of the lane. Lane 3 : Sample library after ExoSAP-IT treatment at 37°C. Lane 4 : Sample library after ExoSAP-IT treatment at 37°C, followed by heat inactivation of the ExoSAP-IT at 80°C. Note the higher molecular weight smear in the methylation library, which is not observed with gDNA amplicons. Lane 5 : Sample library cycled at 37°C, followed by 80°C heat denaturation step, but with no ExoSAP-IT.

    Journal: Clinical Epigenetics

    Article Title: Multiplex bisulfite PCR resequencing of clinical FFPE DNA

    doi: 10.1186/s13148-015-0067-3

    Figure Lengend Snippet: Pre-amplification multiplex results for bisulfite DNA samples. (A) A flow diagram outlining the different conditions examined with respect to multiplexability, pooling, and exonuclease treatment. (B) The results of different conditions after 15 cycles of multiplex pre-amplification. Forty-eight primer pairs were assessed for the multiplexability in a pre-amplification reaction. All samples had the same amount of input DNA. Lane 1 : A positive control involving singleplex PCR reaction of an individual primer pair, with the same DNA template amount as used in the pre-amplification. Lane 2 : Eight-plex pre-amplification reaction and ExoSAP-IT treatment of individual pre-amp reactions, followed by pooling and singleplex amplification (as illustrated in the upper panel of A). Lane 3 : Results of three different 8-plex reactions pooled together first then ExoSAP-IT treatment of the combined pool, for a total of 24 amplicons in the ExoSAP-IT treatment, followed by singleplex amplification of a primer pair (as illustrated in the lower panel of A). Lane 4 : Twenty-four-plex pre-amplification results. (C) The effect of ExoSAP-IT treatment on bisulfite libraries, as compared to gDNA amplicons. The arrow indicates where primers migrate on the gel. Lane 1 : Pool of 48 amplicons prior to barcoding PCR. Lane 2 : Sample library after barcoding PCR. Primers are visible at the bottom of the lane. Lane 3 : Sample library after ExoSAP-IT treatment at 37°C. Lane 4 : Sample library after ExoSAP-IT treatment at 37°C, followed by heat inactivation of the ExoSAP-IT at 80°C. Note the higher molecular weight smear in the methylation library, which is not observed with gDNA amplicons. Lane 5 : Sample library cycled at 37°C, followed by 80°C heat denaturation step, but with no ExoSAP-IT.

    Article Snippet: Our extensive optimization experiments indicated that a template of moderately high quality would require approximately 20 cycles of multiplex pre-amplification, another 35 cycles of singleplex amplification on the Fluidigm Access Array, and finally an additional 15 cycles of barcoding PCR, for a total of 70 cycles of amplification.

    Techniques: Amplification, Multiplex Assay, Flow Cytometry, Positive Control, Polymerase Chain Reaction, Molecular Weight, Methylation