single-stranded cdna Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Integrated DNA Technologies complementary single stranded dna oligonucleotides
    Complementary Single Stranded Dna Oligonucleotides, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complementary single stranded dna oligonucleotides/product/Integrated DNA Technologies
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    complementary single stranded dna oligonucleotides - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher single stranded cdna
    Single Stranded Cdna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 11572 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/single stranded cdna/product/Thermo Fisher
    Average 99 stars, based on 11572 article reviews
    Price from $9.99 to $1999.99
    single stranded cdna - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    92
    Bio-Rad single stranded cdna
    Expression of Cdx-2 and GAPDH mRNA by conventional <t>PCR</t> amplifications. <t>cDNA</t> was synthesized from 50 ng of RNA extracted from small intestine (SI), IPI-2I, and SJPL cells. After 30 cycles of amplification, the PCR products were run on a 1.5% agarose gel and stained with ethidium bromide. Three DNA molecular weight markers were used (MW1 from Amersham and MW2, MW3 from Fermentas). The PCR product sizes are presented in Table 1 .
    Single Stranded Cdna, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1139 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/single stranded cdna/product/Bio-Rad
    Average 92 stars, based on 1139 article reviews
    Price from $9.99 to $1999.99
    single stranded cdna - by Bioz Stars, 2020-10
    92/100 stars
      Buy from Supplier

    93
    Illumina Inc single stranded cdna
    Expression of Cdx-2 and GAPDH mRNA by conventional <t>PCR</t> amplifications. <t>cDNA</t> was synthesized from 50 ng of RNA extracted from small intestine (SI), IPI-2I, and SJPL cells. After 30 cycles of amplification, the PCR products were run on a 1.5% agarose gel and stained with ethidium bromide. Three DNA molecular weight markers were used (MW1 from Amersham and MW2, MW3 from Fermentas). The PCR product sizes are presented in Table 1 .
    Single Stranded Cdna, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 239 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/single stranded cdna/product/Illumina Inc
    Average 93 stars, based on 239 article reviews
    Price from $9.99 to $1999.99
    single stranded cdna - by Bioz Stars, 2020-10
    93/100 stars
      Buy from Supplier

    88
    Bio-Rad single strand complementary dna synthesis
    VPA and NAM treatment modulates H3K27 acetylation at the promoters of myeloid specific genes. UCB-derived CD34+ cells were differentiated towards megakaryocytes. At day 4 of differentiation, cells were treated overnight with 200μM, or 5mM NAM. Next, lysates were prepared, followed by ChIP-sequencing (see Methods ). Data represent a Venn diagram comparing the number of genes identified based on a > 2-fold increase or decrease in H3K27 acetylation levels compared to the control (A). Gene ontology analysis of up- and down-regulated genes by VPA (B) and NAM (C) treatment. From the same cells, <t>RNA</t> was isolated, followed by <t>cDNA</t> synthesis and qPCR (see Methods ). Data represent the relative mRNA level of GATA1, RUNX1, LMO2 and RUNX2 compared to the control (D). Data are representative for 3 experiments. Error bars represent SEM, * p
    Single Strand Complementary Dna Synthesis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 88/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/single strand complementary dna synthesis/product/Bio-Rad
    Average 88 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    single strand complementary dna synthesis - by Bioz Stars, 2020-10
    88/100 stars
      Buy from Supplier

    92
    Nugen single stranded cdna
    VPA and NAM treatment modulates H3K27 acetylation at the promoters of myeloid specific genes. UCB-derived CD34+ cells were differentiated towards megakaryocytes. At day 4 of differentiation, cells were treated overnight with 200μM, or 5mM NAM. Next, lysates were prepared, followed by ChIP-sequencing (see Methods ). Data represent a Venn diagram comparing the number of genes identified based on a > 2-fold increase or decrease in H3K27 acetylation levels compared to the control (A). Gene ontology analysis of up- and down-regulated genes by VPA (B) and NAM (C) treatment. From the same cells, <t>RNA</t> was isolated, followed by <t>cDNA</t> synthesis and qPCR (see Methods ). Data represent the relative mRNA level of GATA1, RUNX1, LMO2 and RUNX2 compared to the control (D). Data are representative for 3 experiments. Error bars represent SEM, * p
    Single Stranded Cdna, supplied by Nugen, used in various techniques. Bioz Stars score: 92/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/single stranded cdna/product/Nugen
    Average 92 stars, based on 69 article reviews
    Price from $9.99 to $1999.99
    single stranded cdna - by Bioz Stars, 2020-10
    92/100 stars
      Buy from Supplier

    92
    Stratagene single strand cdna
    VPA and NAM treatment modulates H3K27 acetylation at the promoters of myeloid specific genes. UCB-derived CD34+ cells were differentiated towards megakaryocytes. At day 4 of differentiation, cells were treated overnight with 200μM, or 5mM NAM. Next, lysates were prepared, followed by ChIP-sequencing (see Methods ). Data represent a Venn diagram comparing the number of genes identified based on a > 2-fold increase or decrease in H3K27 acetylation levels compared to the control (A). Gene ontology analysis of up- and down-regulated genes by VPA (B) and NAM (C) treatment. From the same cells, <t>RNA</t> was isolated, followed by <t>cDNA</t> synthesis and qPCR (see Methods ). Data represent the relative mRNA level of GATA1, RUNX1, LMO2 and RUNX2 compared to the control (D). Data are representative for 3 experiments. Error bars represent SEM, * p
    Single Strand Cdna, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/single strand cdna/product/Stratagene
    Average 92 stars, based on 31 article reviews
    Price from $9.99 to $1999.99
    single strand cdna - by Bioz Stars, 2020-10
    92/100 stars
      Buy from Supplier

    88
    Thermo Fisher single stranded cdna synthesis kit
    VPA and NAM treatment modulates H3K27 acetylation at the promoters of myeloid specific genes. UCB-derived CD34+ cells were differentiated towards megakaryocytes. At day 4 of differentiation, cells were treated overnight with 200μM, or 5mM NAM. Next, lysates were prepared, followed by ChIP-sequencing (see Methods ). Data represent a Venn diagram comparing the number of genes identified based on a > 2-fold increase or decrease in H3K27 acetylation levels compared to the control (A). Gene ontology analysis of up- and down-regulated genes by VPA (B) and NAM (C) treatment. From the same cells, <t>RNA</t> was isolated, followed by <t>cDNA</t> synthesis and qPCR (see Methods ). Data represent the relative mRNA level of GATA1, RUNX1, LMO2 and RUNX2 compared to the control (D). Data are representative for 3 experiments. Error bars represent SEM, * p
    Single Stranded Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/single stranded cdna synthesis kit/product/Thermo Fisher
    Average 88 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    single stranded cdna synthesis kit - by Bioz Stars, 2020-10
    88/100 stars
      Buy from Supplier

    99
    Thermo Fisher complementary single stranded dna oligos
    VPA and NAM treatment modulates H3K27 acetylation at the promoters of myeloid specific genes. UCB-derived CD34+ cells were differentiated towards megakaryocytes. At day 4 of differentiation, cells were treated overnight with 200μM, or 5mM NAM. Next, lysates were prepared, followed by ChIP-sequencing (see Methods ). Data represent a Venn diagram comparing the number of genes identified based on a > 2-fold increase or decrease in H3K27 acetylation levels compared to the control (A). Gene ontology analysis of up- and down-regulated genes by VPA (B) and NAM (C) treatment. From the same cells, <t>RNA</t> was isolated, followed by <t>cDNA</t> synthesis and qPCR (see Methods ). Data represent the relative mRNA level of GATA1, RUNX1, LMO2 and RUNX2 compared to the control (D). Data are representative for 3 experiments. Error bars represent SEM, * p
    Complementary Single Stranded Dna Oligos, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complementary single stranded dna oligos/product/Thermo Fisher
    Average 99 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    complementary single stranded dna oligos - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    94
    Promega single stranded cdna
    VPA and NAM treatment modulates H3K27 acetylation at the promoters of myeloid specific genes. UCB-derived CD34+ cells were differentiated towards megakaryocytes. At day 4 of differentiation, cells were treated overnight with 200μM, or 5mM NAM. Next, lysates were prepared, followed by ChIP-sequencing (see Methods ). Data represent a Venn diagram comparing the number of genes identified based on a > 2-fold increase or decrease in H3K27 acetylation levels compared to the control (A). Gene ontology analysis of up- and down-regulated genes by VPA (B) and NAM (C) treatment. From the same cells, <t>RNA</t> was isolated, followed by <t>cDNA</t> synthesis and qPCR (see Methods ). Data represent the relative mRNA level of GATA1, RUNX1, LMO2 and RUNX2 compared to the control (D). Data are representative for 3 experiments. Error bars represent SEM, * p
    Single Stranded Cdna, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 1517 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/single stranded cdna/product/Promega
    Average 94 stars, based on 1517 article reviews
    Price from $9.99 to $1999.99
    single stranded cdna - by Bioz Stars, 2020-10
    94/100 stars
      Buy from Supplier

    86
    Promega improm ii single strand cdna system
    VPA and NAM treatment modulates H3K27 acetylation at the promoters of myeloid specific genes. UCB-derived CD34+ cells were differentiated towards megakaryocytes. At day 4 of differentiation, cells were treated overnight with 200μM, or 5mM NAM. Next, lysates were prepared, followed by ChIP-sequencing (see Methods ). Data represent a Venn diagram comparing the number of genes identified based on a > 2-fold increase or decrease in H3K27 acetylation levels compared to the control (A). Gene ontology analysis of up- and down-regulated genes by VPA (B) and NAM (C) treatment. From the same cells, <t>RNA</t> was isolated, followed by <t>cDNA</t> synthesis and qPCR (see Methods ). Data represent the relative mRNA level of GATA1, RUNX1, LMO2 and RUNX2 compared to the control (D). Data are representative for 3 experiments. Error bars represent SEM, * p
    Improm Ii Single Strand Cdna System, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/improm ii single strand cdna system/product/Promega
    Average 86 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    improm ii single strand cdna system - by Bioz Stars, 2020-10
    86/100 stars
      Buy from Supplier

    99
    GE Healthcare single strand cdna synthesis kit
    VPA and NAM treatment modulates H3K27 acetylation at the promoters of myeloid specific genes. UCB-derived CD34+ cells were differentiated towards megakaryocytes. At day 4 of differentiation, cells were treated overnight with 200μM, or 5mM NAM. Next, lysates were prepared, followed by ChIP-sequencing (see Methods ). Data represent a Venn diagram comparing the number of genes identified based on a > 2-fold increase or decrease in H3K27 acetylation levels compared to the control (A). Gene ontology analysis of up- and down-regulated genes by VPA (B) and NAM (C) treatment. From the same cells, <t>RNA</t> was isolated, followed by <t>cDNA</t> synthesis and qPCR (see Methods ). Data represent the relative mRNA level of GATA1, RUNX1, LMO2 and RUNX2 compared to the control (D). Data are representative for 3 experiments. Error bars represent SEM, * p
    Single Strand Cdna Synthesis Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/single strand cdna synthesis kit/product/GE Healthcare
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    single strand cdna synthesis kit - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    91
    Thermo Fisher single stranded cdna synthesis
    VPA and NAM treatment modulates H3K27 acetylation at the promoters of myeloid specific genes. UCB-derived CD34+ cells were differentiated towards megakaryocytes. At day 4 of differentiation, cells were treated overnight with 200μM, or 5mM NAM. Next, lysates were prepared, followed by ChIP-sequencing (see Methods ). Data represent a Venn diagram comparing the number of genes identified based on a > 2-fold increase or decrease in H3K27 acetylation levels compared to the control (A). Gene ontology analysis of up- and down-regulated genes by VPA (B) and NAM (C) treatment. From the same cells, <t>RNA</t> was isolated, followed by <t>cDNA</t> synthesis and qPCR (see Methods ). Data represent the relative mRNA level of GATA1, RUNX1, LMO2 and RUNX2 compared to the control (D). Data are representative for 3 experiments. Error bars represent SEM, * p
    Single Stranded Cdna Synthesis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 482 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/single stranded cdna synthesis/product/Thermo Fisher
    Average 91 stars, based on 482 article reviews
    Price from $9.99 to $1999.99
    single stranded cdna synthesis - by Bioz Stars, 2020-10
    91/100 stars
      Buy from Supplier

    88
    Thermo Fisher labeled single stranded cdna
    VPA and NAM treatment modulates H3K27 acetylation at the promoters of myeloid specific genes. UCB-derived CD34+ cells were differentiated towards megakaryocytes. At day 4 of differentiation, cells were treated overnight with 200μM, or 5mM NAM. Next, lysates were prepared, followed by ChIP-sequencing (see Methods ). Data represent a Venn diagram comparing the number of genes identified based on a > 2-fold increase or decrease in H3K27 acetylation levels compared to the control (A). Gene ontology analysis of up- and down-regulated genes by VPA (B) and NAM (C) treatment. From the same cells, <t>RNA</t> was isolated, followed by <t>cDNA</t> synthesis and qPCR (see Methods ). Data represent the relative mRNA level of GATA1, RUNX1, LMO2 and RUNX2 compared to the control (D). Data are representative for 3 experiments. Error bars represent SEM, * p
    Labeled Single Stranded Cdna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 165 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/labeled single stranded cdna/product/Thermo Fisher
    Average 88 stars, based on 165 article reviews
    Price from $9.99 to $1999.99
    labeled single stranded cdna - by Bioz Stars, 2020-10
    88/100 stars
      Buy from Supplier

    84
    Eurofins complementary single strand hplc purified dnas
    VPA and NAM treatment modulates H3K27 acetylation at the promoters of myeloid specific genes. UCB-derived CD34+ cells were differentiated towards megakaryocytes. At day 4 of differentiation, cells were treated overnight with 200μM, or 5mM NAM. Next, lysates were prepared, followed by ChIP-sequencing (see Methods ). Data represent a Venn diagram comparing the number of genes identified based on a > 2-fold increase or decrease in H3K27 acetylation levels compared to the control (A). Gene ontology analysis of up- and down-regulated genes by VPA (B) and NAM (C) treatment. From the same cells, <t>RNA</t> was isolated, followed by <t>cDNA</t> synthesis and qPCR (see Methods ). Data represent the relative mRNA level of GATA1, RUNX1, LMO2 and RUNX2 compared to the control (D). Data are representative for 3 experiments. Error bars represent SEM, * p
    Complementary Single Strand Hplc Purified Dnas, supplied by Eurofins, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complementary single strand hplc purified dnas/product/Eurofins
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    complementary single strand hplc purified dnas - by Bioz Stars, 2020-10
    84/100 stars
      Buy from Supplier

    99
    Thermo Fisher single stranded dna complementary
    VPA and NAM treatment modulates H3K27 acetylation at the promoters of myeloid specific genes. UCB-derived CD34+ cells were differentiated towards megakaryocytes. At day 4 of differentiation, cells were treated overnight with 200μM, or 5mM NAM. Next, lysates were prepared, followed by ChIP-sequencing (see Methods ). Data represent a Venn diagram comparing the number of genes identified based on a > 2-fold increase or decrease in H3K27 acetylation levels compared to the control (A). Gene ontology analysis of up- and down-regulated genes by VPA (B) and NAM (C) treatment. From the same cells, <t>RNA</t> was isolated, followed by <t>cDNA</t> synthesis and qPCR (see Methods ). Data represent the relative mRNA level of GATA1, RUNX1, LMO2 and RUNX2 compared to the control (D). Data are representative for 3 experiments. Error bars represent SEM, * p
    Single Stranded Dna Complementary, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/single stranded dna complementary/product/Thermo Fisher
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    single stranded dna complementary - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher first strand cdna synthesis kit
    VPA and NAM treatment modulates H3K27 acetylation at the promoters of myeloid specific genes. UCB-derived CD34+ cells were differentiated towards megakaryocytes. At day 4 of differentiation, cells were treated overnight with 200μM, or 5mM NAM. Next, lysates were prepared, followed by ChIP-sequencing (see Methods ). Data represent a Venn diagram comparing the number of genes identified based on a > 2-fold increase or decrease in H3K27 acetylation levels compared to the control (A). Gene ontology analysis of up- and down-regulated genes by VPA (B) and NAM (C) treatment. From the same cells, <t>RNA</t> was isolated, followed by <t>cDNA</t> synthesis and qPCR (see Methods ). Data represent the relative mRNA level of GATA1, RUNX1, LMO2 and RUNX2 compared to the control (D). Data are representative for 3 experiments. Error bars represent SEM, * p
    First Strand Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 20160 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand cdna synthesis kit/product/Thermo Fisher
    Average 99 stars, based on 20160 article reviews
    Price from $9.99 to $1999.99
    first strand cdna synthesis kit - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    89
    Promega improm iitm single strand cdna synthesis kit
    VPA and NAM treatment modulates H3K27 acetylation at the promoters of myeloid specific genes. UCB-derived CD34+ cells were differentiated towards megakaryocytes. At day 4 of differentiation, cells were treated overnight with 200μM, or 5mM NAM. Next, lysates were prepared, followed by ChIP-sequencing (see Methods ). Data represent a Venn diagram comparing the number of genes identified based on a > 2-fold increase or decrease in H3K27 acetylation levels compared to the control (A). Gene ontology analysis of up- and down-regulated genes by VPA (B) and NAM (C) treatment. From the same cells, <t>RNA</t> was isolated, followed by <t>cDNA</t> synthesis and qPCR (see Methods ). Data represent the relative mRNA level of GATA1, RUNX1, LMO2 and RUNX2 compared to the control (D). Data are representative for 3 experiments. Error bars represent SEM, * p
    Improm Iitm Single Strand Cdna Synthesis Kit, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/improm iitm single strand cdna synthesis kit/product/Promega
    Average 89 stars, based on 48 article reviews
    Price from $9.99 to $1999.99
    improm iitm single strand cdna synthesis kit - by Bioz Stars, 2020-10
    89/100 stars
      Buy from Supplier

    Image Search Results


    Expression of Cdx-2 and GAPDH mRNA by conventional PCR amplifications. cDNA was synthesized from 50 ng of RNA extracted from small intestine (SI), IPI-2I, and SJPL cells. After 30 cycles of amplification, the PCR products were run on a 1.5% agarose gel and stained with ethidium bromide. Three DNA molecular weight markers were used (MW1 from Amersham and MW2, MW3 from Fermentas). The PCR product sizes are presented in Table 1 .

    Journal: PLoS ONE

    Article Title: Commensal Bacteria and Expression of Two Major Intestinal Chemokines, TECK/CCL25 and MEC/CCL28, and Their Receptors

    doi: 10.1371/journal.pone.0000677

    Figure Lengend Snippet: Expression of Cdx-2 and GAPDH mRNA by conventional PCR amplifications. cDNA was synthesized from 50 ng of RNA extracted from small intestine (SI), IPI-2I, and SJPL cells. After 30 cycles of amplification, the PCR products were run on a 1.5% agarose gel and stained with ethidium bromide. Three DNA molecular weight markers were used (MW1 from Amersham and MW2, MW3 from Fermentas). The PCR product sizes are presented in Table 1 .

    Article Snippet: The resulting single-stranded cDNA was then used in for real-time PCR (qPCR) analysis (iCycler iQ Real-Time PCR detection system, Bio-Rad, Hercules, CA) for evaluation of relative expression. cDNA was combined with primer/probe sets and IQ SYBR Green Supermix (Bio Rad) according to the manufacturer's recommendations.

    Techniques: Expressing, Polymerase Chain Reaction, Synthesized, Amplification, Agarose Gel Electrophoresis, Staining, Molecular Weight

    VPA and NAM treatment modulates H3K27 acetylation at the promoters of myeloid specific genes. UCB-derived CD34+ cells were differentiated towards megakaryocytes. At day 4 of differentiation, cells were treated overnight with 200μM, or 5mM NAM. Next, lysates were prepared, followed by ChIP-sequencing (see Methods ). Data represent a Venn diagram comparing the number of genes identified based on a > 2-fold increase or decrease in H3K27 acetylation levels compared to the control (A). Gene ontology analysis of up- and down-regulated genes by VPA (B) and NAM (C) treatment. From the same cells, RNA was isolated, followed by cDNA synthesis and qPCR (see Methods ). Data represent the relative mRNA level of GATA1, RUNX1, LMO2 and RUNX2 compared to the control (D). Data are representative for 3 experiments. Error bars represent SEM, * p

    Journal: PLoS ONE

    Article Title: Megakaryocyte lineage development is controlled by modulation of protein acetylation

    doi: 10.1371/journal.pone.0196400

    Figure Lengend Snippet: VPA and NAM treatment modulates H3K27 acetylation at the promoters of myeloid specific genes. UCB-derived CD34+ cells were differentiated towards megakaryocytes. At day 4 of differentiation, cells were treated overnight with 200μM, or 5mM NAM. Next, lysates were prepared, followed by ChIP-sequencing (see Methods ). Data represent a Venn diagram comparing the number of genes identified based on a > 2-fold increase or decrease in H3K27 acetylation levels compared to the control (A). Gene ontology analysis of up- and down-regulated genes by VPA (B) and NAM (C) treatment. From the same cells, RNA was isolated, followed by cDNA synthesis and qPCR (see Methods ). Data represent the relative mRNA level of GATA1, RUNX1, LMO2 and RUNX2 compared to the control (D). Data are representative for 3 experiments. Error bars represent SEM, * p

    Article Snippet: According to the manufactures protocol for single-stranded cDNA synthesis, 500 ng of total RNA was reverse transcribed using iScript cDNA synthesis kit (BIO-Rad, Hercules, CA). cDNA samples were amplified using SYBR green supermix (BIO-Rad), in a MyiQ single-color real time PCR detection system (BIO-Rad) according to the manufacturers’ protocol.

    Techniques: Derivative Assay, Chromatin Immunoprecipitation, Sequencing, Isolation, Real-time Polymerase Chain Reaction