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  • 99
    Thermo Fisher synthesis single strand cdna
    Synthesis Single Strand Cdna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa single strand complementary dna cdna
    <t>RT–PCR</t> analysis to test for the presence of ey HD and molecular analysis of the ey mutants. ( A–C ) RT–PCR on leg discs of third instar larval stage in which ey and eyΔHD were misexpressed. (Lanes 1–3 ) yw controls. (Lane 1 ) Eye; (lane 2 ) wing; (lane 3 ) leg discs; (lane 4 ) misexpression of full-length ey ; (lane 5 ) ey <t>cDNA</t> control; (lane 6 ) misexpression of eyΔHD ; (lane 7 ) eyΔHD cDNA control; (lane 8 ) water control. ( A ) Detection of full-length ey , primer 1 + 2 of D . ( B ) Detection of ey HD fragment, primer 1 + 3 of D . ( C ) rp49 control. In lane 6 , in which eyΔHD was misexpressed, we failed to detect full-length ey ( A ) and the HD fragment ( B ), whereas rp49 was expressed normally. ( D ) Schematic drawing of ey cDNA and the respectively used primers for the RT–PCR in A and B . ( E,F ) Molecular analysis of ey mutants. ( E , lanes 1–5 ) Western blot analysis of ey 2 mutants of third instar larval stage with an anti PD antibody; (lanes 1,2 ) eye discs of wild-type and ey 2 , respectively. EY is only detectable in wild-type in contrast to TOY. (Lanes 3,4 ) Wing and leg discs control of wild type; (lane 5 ) larval brain of wild type, both proteins are expressed. ( F , lanes 1–3 ): Western blot analysis of adult head with an anti EY antibody. (Lane 1) Wild type; (lane 2 ) ey 2 ; (lane 3 ) ey J5.71 . EY could be detected in wild type and ey 2 , but not in ey J5.71 .
    Single Strand Complementary Dna Cdna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche single strand complementary dna cdna
    <t>RT–PCR</t> analysis to test for the presence of ey HD and molecular analysis of the ey mutants. ( A–C ) RT–PCR on leg discs of third instar larval stage in which ey and eyΔHD were misexpressed. (Lanes 1–3 ) yw controls. (Lane 1 ) Eye; (lane 2 ) wing; (lane 3 ) leg discs; (lane 4 ) misexpression of full-length ey ; (lane 5 ) ey <t>cDNA</t> control; (lane 6 ) misexpression of eyΔHD ; (lane 7 ) eyΔHD cDNA control; (lane 8 ) water control. ( A ) Detection of full-length ey , primer 1 + 2 of D . ( B ) Detection of ey HD fragment, primer 1 + 3 of D . ( C ) rp49 control. In lane 6 , in which eyΔHD was misexpressed, we failed to detect full-length ey ( A ) and the HD fragment ( B ), whereas rp49 was expressed normally. ( D ) Schematic drawing of ey cDNA and the respectively used primers for the RT–PCR in A and B . ( E,F ) Molecular analysis of ey mutants. ( E , lanes 1–5 ) Western blot analysis of ey 2 mutants of third instar larval stage with an anti PD antibody; (lanes 1,2 ) eye discs of wild-type and ey 2 , respectively. EY is only detectable in wild-type in contrast to TOY. (Lanes 3,4 ) Wing and leg discs control of wild type; (lane 5 ) larval brain of wild type, both proteins are expressed. ( F , lanes 1–3 ): Western blot analysis of adult head with an anti EY antibody. (Lane 1) Wild type; (lane 2 ) ey 2 ; (lane 3 ) ey J5.71 . EY could be detected in wild type and ey 2 , but not in ey J5.71 .
    Single Strand Complementary Dna Cdna, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad single stranded complementary dna cdna
    <t>RT–PCR</t> analysis to test for the presence of ey HD and molecular analysis of the ey mutants. ( A–C ) RT–PCR on leg discs of third instar larval stage in which ey and eyΔHD were misexpressed. (Lanes 1–3 ) yw controls. (Lane 1 ) Eye; (lane 2 ) wing; (lane 3 ) leg discs; (lane 4 ) misexpression of full-length ey ; (lane 5 ) ey <t>cDNA</t> control; (lane 6 ) misexpression of eyΔHD ; (lane 7 ) eyΔHD cDNA control; (lane 8 ) water control. ( A ) Detection of full-length ey , primer 1 + 2 of D . ( B ) Detection of ey HD fragment, primer 1 + 3 of D . ( C ) rp49 control. In lane 6 , in which eyΔHD was misexpressed, we failed to detect full-length ey ( A ) and the HD fragment ( B ), whereas rp49 was expressed normally. ( D ) Schematic drawing of ey cDNA and the respectively used primers for the RT–PCR in A and B . ( E,F ) Molecular analysis of ey mutants. ( E , lanes 1–5 ) Western blot analysis of ey 2 mutants of third instar larval stage with an anti PD antibody; (lanes 1,2 ) eye discs of wild-type and ey 2 , respectively. EY is only detectable in wild-type in contrast to TOY. (Lanes 3,4 ) Wing and leg discs control of wild type; (lane 5 ) larval brain of wild type, both proteins are expressed. ( F , lanes 1–3 ): Western blot analysis of adult head with an anti EY antibody. (Lane 1) Wild type; (lane 2 ) ey 2 ; (lane 3 ) ey J5.71 . EY could be detected in wild type and ey 2 , but not in ey J5.71 .
    Single Stranded Complementary Dna Cdna, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega single stranded complementary dna cdna
    <t>RT–PCR</t> analysis to test for the presence of ey HD and molecular analysis of the ey mutants. ( A–C ) RT–PCR on leg discs of third instar larval stage in which ey and eyΔHD were misexpressed. (Lanes 1–3 ) yw controls. (Lane 1 ) Eye; (lane 2 ) wing; (lane 3 ) leg discs; (lane 4 ) misexpression of full-length ey ; (lane 5 ) ey <t>cDNA</t> control; (lane 6 ) misexpression of eyΔHD ; (lane 7 ) eyΔHD cDNA control; (lane 8 ) water control. ( A ) Detection of full-length ey , primer 1 + 2 of D . ( B ) Detection of ey HD fragment, primer 1 + 3 of D . ( C ) rp49 control. In lane 6 , in which eyΔHD was misexpressed, we failed to detect full-length ey ( A ) and the HD fragment ( B ), whereas rp49 was expressed normally. ( D ) Schematic drawing of ey cDNA and the respectively used primers for the RT–PCR in A and B . ( E,F ) Molecular analysis of ey mutants. ( E , lanes 1–5 ) Western blot analysis of ey 2 mutants of third instar larval stage with an anti PD antibody; (lanes 1,2 ) eye discs of wild-type and ey 2 , respectively. EY is only detectable in wild-type in contrast to TOY. (Lanes 3,4 ) Wing and leg discs control of wild type; (lane 5 ) larval brain of wild type, both proteins are expressed. ( F , lanes 1–3 ): Western blot analysis of adult head with an anti EY antibody. (Lane 1) Wild type; (lane 2 ) ey 2 ; (lane 3 ) ey J5.71 . EY could be detected in wild type and ey 2 , but not in ey J5.71 .
    Single Stranded Complementary Dna Cdna, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher single stranded complementary dna cdna
    Tissue distribution of human prepro-UII mRNA. ( A ) Dot blot analysis of prepro-UII mRNA expression in various human tissues. The master blot (CLONTECH) consisted of 50 human-tissue poly(A) RNAs (80–448 ng per dot) normalized by using the <t>RNA</t> expression levels of eight housekeeping genes. Positive controls consisted of human genomic DNA. Negative controls included yeast and Escherichia coli RNA or DNA, as well as human repetitive genomic sequences. The blot was probed with the human prepro-UII <t>cDNA</t> probe and exposed for 2 days onto an X-Omat film ( B ) Northern blot analysis of prepro-UII mRNA expression in the human spinal cord. Spinal cord poly(A) mRNA (2 μg) was hybridized with the human prepro-UII cDNA probe. The molecular weight was determined by using RNA markers. ( C ) X-ray autoradiographs showing the distribution of prepro-UII mRNA in the human spinal cord. Coronal sections were hybridized with the antisense ( Top ) or sense ( Bottom ) prepro-UII riboprobe and exposed for 10 days onto x-ray film.
    Single Stranded Complementary Dna Cdna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 719 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nugen single stranded complementary dna cdna
    Tissue distribution of human prepro-UII mRNA. ( A ) Dot blot analysis of prepro-UII mRNA expression in various human tissues. The master blot (CLONTECH) consisted of 50 human-tissue poly(A) RNAs (80–448 ng per dot) normalized by using the <t>RNA</t> expression levels of eight housekeeping genes. Positive controls consisted of human genomic DNA. Negative controls included yeast and Escherichia coli RNA or DNA, as well as human repetitive genomic sequences. The blot was probed with the human prepro-UII <t>cDNA</t> probe and exposed for 2 days onto an X-Omat film ( B ) Northern blot analysis of prepro-UII mRNA expression in the human spinal cord. Spinal cord poly(A) mRNA (2 μg) was hybridized with the human prepro-UII cDNA probe. The molecular weight was determined by using RNA markers. ( C ) X-ray autoradiographs showing the distribution of prepro-UII mRNA in the human spinal cord. Coronal sections were hybridized with the antisense ( Top ) or sense ( Bottom ) prepro-UII riboprobe and exposed for 10 days onto x-ray film.
    Single Stranded Complementary Dna Cdna, supplied by Nugen, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche single strand complementary dna cdna synthesis
    Tissue distribution of human prepro-UII mRNA. ( A ) Dot blot analysis of prepro-UII mRNA expression in various human tissues. The master blot (CLONTECH) consisted of 50 human-tissue poly(A) RNAs (80–448 ng per dot) normalized by using the <t>RNA</t> expression levels of eight housekeeping genes. Positive controls consisted of human genomic DNA. Negative controls included yeast and Escherichia coli RNA or DNA, as well as human repetitive genomic sequences. The blot was probed with the human prepro-UII <t>cDNA</t> probe and exposed for 2 days onto an X-Omat film ( B ) Northern blot analysis of prepro-UII mRNA expression in the human spinal cord. Spinal cord poly(A) mRNA (2 μg) was hybridized with the human prepro-UII cDNA probe. The molecular weight was determined by using RNA markers. ( C ) X-ray autoradiographs showing the distribution of prepro-UII mRNA in the human spinal cord. Coronal sections were hybridized with the antisense ( Top ) or sense ( Bottom ) prepro-UII riboprobe and exposed for 10 days onto x-ray film.
    Single Strand Complementary Dna Cdna Synthesis, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad single strand complementary dna cdna synthesis
    Tissue distribution of human prepro-UII mRNA. ( A ) Dot blot analysis of prepro-UII mRNA expression in various human tissues. The master blot (CLONTECH) consisted of 50 human-tissue poly(A) RNAs (80–448 ng per dot) normalized by using the <t>RNA</t> expression levels of eight housekeeping genes. Positive controls consisted of human genomic DNA. Negative controls included yeast and Escherichia coli RNA or DNA, as well as human repetitive genomic sequences. The blot was probed with the human prepro-UII <t>cDNA</t> probe and exposed for 2 days onto an X-Omat film ( B ) Northern blot analysis of prepro-UII mRNA expression in the human spinal cord. Spinal cord poly(A) mRNA (2 μg) was hybridized with the human prepro-UII cDNA probe. The molecular weight was determined by using RNA markers. ( C ) X-ray autoradiographs showing the distribution of prepro-UII mRNA in the human spinal cord. Coronal sections were hybridized with the antisense ( Top ) or sense ( Bottom ) prepro-UII riboprobe and exposed for 10 days onto x-ray film.
    Single Strand Complementary Dna Cdna Synthesis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher single stranded cdna synthesis
    Tissue distribution of human prepro-UII mRNA. ( A ) Dot blot analysis of prepro-UII mRNA expression in various human tissues. The master blot (CLONTECH) consisted of 50 human-tissue poly(A) RNAs (80–448 ng per dot) normalized by using the <t>RNA</t> expression levels of eight housekeeping genes. Positive controls consisted of human genomic DNA. Negative controls included yeast and Escherichia coli RNA or DNA, as well as human repetitive genomic sequences. The blot was probed with the human prepro-UII <t>cDNA</t> probe and exposed for 2 days onto an X-Omat film ( B ) Northern blot analysis of prepro-UII mRNA expression in the human spinal cord. Spinal cord poly(A) mRNA (2 μg) was hybridized with the human prepro-UII cDNA probe. The molecular weight was determined by using RNA markers. ( C ) X-ray autoradiographs showing the distribution of prepro-UII mRNA in the human spinal cord. Coronal sections were hybridized with the antisense ( Top ) or sense ( Bottom ) prepro-UII riboprobe and exposed for 10 days onto x-ray film.
    Single Stranded Cdna Synthesis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 403 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega single strand cdna synthesis kit
    Tissue distribution of human prepro-UII mRNA. ( A ) Dot blot analysis of prepro-UII mRNA expression in various human tissues. The master blot (CLONTECH) consisted of 50 human-tissue poly(A) RNAs (80–448 ng per dot) normalized by using the <t>RNA</t> expression levels of eight housekeeping genes. Positive controls consisted of human genomic DNA. Negative controls included yeast and Escherichia coli RNA or DNA, as well as human repetitive genomic sequences. The blot was probed with the human prepro-UII <t>cDNA</t> probe and exposed for 2 days onto an X-Omat film ( B ) Northern blot analysis of prepro-UII mRNA expression in the human spinal cord. Spinal cord poly(A) mRNA (2 μg) was hybridized with the human prepro-UII cDNA probe. The molecular weight was determined by using RNA markers. ( C ) X-ray autoradiographs showing the distribution of prepro-UII mRNA in the human spinal cord. Coronal sections were hybridized with the antisense ( Top ) or sense ( Bottom ) prepro-UII riboprobe and exposed for 10 days onto x-ray film.
    Single Strand Cdna Synthesis Kit, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare single strand cdna synthesis kit
    Tissue distribution of human prepro-UII mRNA. ( A ) Dot blot analysis of prepro-UII mRNA expression in various human tissues. The master blot (CLONTECH) consisted of 50 human-tissue poly(A) RNAs (80–448 ng per dot) normalized by using the <t>RNA</t> expression levels of eight housekeeping genes. Positive controls consisted of human genomic DNA. Negative controls included yeast and Escherichia coli RNA or DNA, as well as human repetitive genomic sequences. The blot was probed with the human prepro-UII <t>cDNA</t> probe and exposed for 2 days onto an X-Omat film ( B ) Northern blot analysis of prepro-UII mRNA expression in the human spinal cord. Spinal cord poly(A) mRNA (2 μg) was hybridized with the human prepro-UII cDNA probe. The molecular weight was determined by using RNA markers. ( C ) X-ray autoradiographs showing the distribution of prepro-UII mRNA in the human spinal cord. Coronal sections were hybridized with the antisense ( Top ) or sense ( Bottom ) prepro-UII riboprobe and exposed for 10 days onto x-ray film.
    Single Strand Cdna Synthesis Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bioteke Corporation single strand cdna synthesis kit
    Tissue distribution of human prepro-UII mRNA. ( A ) Dot blot analysis of prepro-UII mRNA expression in various human tissues. The master blot (CLONTECH) consisted of 50 human-tissue poly(A) RNAs (80–448 ng per dot) normalized by using the <t>RNA</t> expression levels of eight housekeeping genes. Positive controls consisted of human genomic DNA. Negative controls included yeast and Escherichia coli RNA or DNA, as well as human repetitive genomic sequences. The blot was probed with the human prepro-UII <t>cDNA</t> probe and exposed for 2 days onto an X-Omat film ( B ) Northern blot analysis of prepro-UII mRNA expression in the human spinal cord. Spinal cord poly(A) mRNA (2 μg) was hybridized with the human prepro-UII cDNA probe. The molecular weight was determined by using RNA markers. ( C ) X-ray autoradiographs showing the distribution of prepro-UII mRNA in the human spinal cord. Coronal sections were hybridized with the antisense ( Top ) or sense ( Bottom ) prepro-UII riboprobe and exposed for 10 days onto x-ray film.
    Single Strand Cdna Synthesis Kit, supplied by Bioteke Corporation, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche single strand cdna synthesis kit
    Tissue distribution of human prepro-UII mRNA. ( A ) Dot blot analysis of prepro-UII mRNA expression in various human tissues. The master blot (CLONTECH) consisted of 50 human-tissue poly(A) RNAs (80–448 ng per dot) normalized by using the <t>RNA</t> expression levels of eight housekeeping genes. Positive controls consisted of human genomic DNA. Negative controls included yeast and Escherichia coli RNA or DNA, as well as human repetitive genomic sequences. The blot was probed with the human prepro-UII <t>cDNA</t> probe and exposed for 2 days onto an X-Omat film ( B ) Northern blot analysis of prepro-UII mRNA expression in the human spinal cord. Spinal cord poly(A) mRNA (2 μg) was hybridized with the human prepro-UII cDNA probe. The molecular weight was determined by using RNA markers. ( C ) X-ray autoradiographs showing the distribution of prepro-UII mRNA in the human spinal cord. Coronal sections were hybridized with the antisense ( Top ) or sense ( Bottom ) prepro-UII riboprobe and exposed for 10 days onto x-ray film.
    Single Strand Cdna Synthesis Kit, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene single strand cdna synthesis kit
    Tissue distribution of human prepro-UII mRNA. ( A ) Dot blot analysis of prepro-UII mRNA expression in various human tissues. The master blot (CLONTECH) consisted of 50 human-tissue poly(A) RNAs (80–448 ng per dot) normalized by using the <t>RNA</t> expression levels of eight housekeeping genes. Positive controls consisted of human genomic DNA. Negative controls included yeast and Escherichia coli RNA or DNA, as well as human repetitive genomic sequences. The blot was probed with the human prepro-UII <t>cDNA</t> probe and exposed for 2 days onto an X-Omat film ( B ) Northern blot analysis of prepro-UII mRNA expression in the human spinal cord. Spinal cord poly(A) mRNA (2 μg) was hybridized with the human prepro-UII cDNA probe. The molecular weight was determined by using RNA markers. ( C ) X-ray autoradiographs showing the distribution of prepro-UII mRNA in the human spinal cord. Coronal sections were hybridized with the antisense ( Top ) or sense ( Bottom ) prepro-UII riboprobe and exposed for 10 days onto x-ray film.
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    Image Search Results


    RT–PCR analysis to test for the presence of ey HD and molecular analysis of the ey mutants. ( A–C ) RT–PCR on leg discs of third instar larval stage in which ey and eyΔHD were misexpressed. (Lanes 1–3 ) yw controls. (Lane 1 ) Eye; (lane 2 ) wing; (lane 3 ) leg discs; (lane 4 ) misexpression of full-length ey ; (lane 5 ) ey cDNA control; (lane 6 ) misexpression of eyΔHD ; (lane 7 ) eyΔHD cDNA control; (lane 8 ) water control. ( A ) Detection of full-length ey , primer 1 + 2 of D . ( B ) Detection of ey HD fragment, primer 1 + 3 of D . ( C ) rp49 control. In lane 6 , in which eyΔHD was misexpressed, we failed to detect full-length ey ( A ) and the HD fragment ( B ), whereas rp49 was expressed normally. ( D ) Schematic drawing of ey cDNA and the respectively used primers for the RT–PCR in A and B . ( E,F ) Molecular analysis of ey mutants. ( E , lanes 1–5 ) Western blot analysis of ey 2 mutants of third instar larval stage with an anti PD antibody; (lanes 1,2 ) eye discs of wild-type and ey 2 , respectively. EY is only detectable in wild-type in contrast to TOY. (Lanes 3,4 ) Wing and leg discs control of wild type; (lane 5 ) larval brain of wild type, both proteins are expressed. ( F , lanes 1–3 ): Western blot analysis of adult head with an anti EY antibody. (Lane 1) Wild type; (lane 2 ) ey 2 ; (lane 3 ) ey J5.71 . EY could be detected in wild type and ey 2 , but not in ey J5.71 .

    Journal: Genes & Development

    Article Title: The eyeless homeodomain is dispensable for eye development in Drosophila

    doi: 10.1101/gad.196401

    Figure Lengend Snippet: RT–PCR analysis to test for the presence of ey HD and molecular analysis of the ey mutants. ( A–C ) RT–PCR on leg discs of third instar larval stage in which ey and eyΔHD were misexpressed. (Lanes 1–3 ) yw controls. (Lane 1 ) Eye; (lane 2 ) wing; (lane 3 ) leg discs; (lane 4 ) misexpression of full-length ey ; (lane 5 ) ey cDNA control; (lane 6 ) misexpression of eyΔHD ; (lane 7 ) eyΔHD cDNA control; (lane 8 ) water control. ( A ) Detection of full-length ey , primer 1 + 2 of D . ( B ) Detection of ey HD fragment, primer 1 + 3 of D . ( C ) rp49 control. In lane 6 , in which eyΔHD was misexpressed, we failed to detect full-length ey ( A ) and the HD fragment ( B ), whereas rp49 was expressed normally. ( D ) Schematic drawing of ey cDNA and the respectively used primers for the RT–PCR in A and B . ( E,F ) Molecular analysis of ey mutants. ( E , lanes 1–5 ) Western blot analysis of ey 2 mutants of third instar larval stage with an anti PD antibody; (lanes 1,2 ) eye discs of wild-type and ey 2 , respectively. EY is only detectable in wild-type in contrast to TOY. (Lanes 3,4 ) Wing and leg discs control of wild type; (lane 5 ) larval brain of wild type, both proteins are expressed. ( F , lanes 1–3 ): Western blot analysis of adult head with an anti EY antibody. (Lane 1) Wild type; (lane 2 ) ey 2 ; (lane 3 ) ey J5.71 . EY could be detected in wild type and ey 2 , but not in ey J5.71 .

    Article Snippet: The single-stranded cDNA was amplified by SMART PCR cDNA Synthesis Kit (Clontech).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot

    Expression profiles of the GENX-769 mRNA. ( Top ) Northern blot analysis of the GENX-769 transcript in 8 normal tissues, 15 regions of brain, and 4 fetal tissues. The insert of cDNA clone c-08e08 (IMAGE-57147) was used as a probe. The size of the transcript is indicated in kb. Each lane contained 2 μg of poly(A) + mRNA from the various human sources. For expression in 15 regions of brain, intensity signals observed on Northern blots was quantitated using ImageQuant software (Molecular Dynamics). To normalize the signal in relation with the quantity of RNA in each lane and compare variations from one tissue to another, each value was divided by those obtained by hybridization of the Northern blots with ubiquitous control actin and ubiquitin cDNA probes to control the amount of mRNA. The signal intensity is represented in arbitrary units. ( Bottom ) In situ hybridization of GENX-769. Rat brain coronal sections through the diencephalon hybridized with antisense ( A ) and sense ( B ) probes. Only the reticular nuclei (RET) in the thalamus (TH) are labeled. A very weak signal is observed in the hippocampus (Ammon’s horn and dentate gyrus). On sections through the cerebellum, labeled cells are localized in the medial vestibular (MV) and superior vestibular (SV) nuclei, and in the medial (ME) and lateral (LAT) cerebellar nuclei.

    Journal: Genome Research

    Article Title: The Genexpress IMAGE Knowledge Base of the Human Brain Transcriptome: A Prototype Integrated Resource for Functional and Computational Genomics

    doi:

    Figure Lengend Snippet: Expression profiles of the GENX-769 mRNA. ( Top ) Northern blot analysis of the GENX-769 transcript in 8 normal tissues, 15 regions of brain, and 4 fetal tissues. The insert of cDNA clone c-08e08 (IMAGE-57147) was used as a probe. The size of the transcript is indicated in kb. Each lane contained 2 μg of poly(A) + mRNA from the various human sources. For expression in 15 regions of brain, intensity signals observed on Northern blots was quantitated using ImageQuant software (Molecular Dynamics). To normalize the signal in relation with the quantity of RNA in each lane and compare variations from one tissue to another, each value was divided by those obtained by hybridization of the Northern blots with ubiquitous control actin and ubiquitin cDNA probes to control the amount of mRNA. The signal intensity is represented in arbitrary units. ( Bottom ) In situ hybridization of GENX-769. Rat brain coronal sections through the diencephalon hybridized with antisense ( A ) and sense ( B ) probes. Only the reticular nuclei (RET) in the thalamus (TH) are labeled. A very weak signal is observed in the hippocampus (Ammon’s horn and dentate gyrus). On sections through the cerebellum, labeled cells are localized in the medial vestibular (MV) and superior vestibular (SV) nuclei, and in the medial (ME) and lateral (LAT) cerebellar nuclei.

    Article Snippet: Single-strand cDNA probes were derived from various poly(A)+ mRNA preparations from human brain, skeletal muscle, heart, placenta, testis, and thymus (Clontech, Palo Alto, CA).

    Techniques: Expressing, Northern Blot, Software, Hybridization, In Situ Hybridization, Labeling

    Tissue distribution of human prepro-UII mRNA. ( A ) Dot blot analysis of prepro-UII mRNA expression in various human tissues. The master blot (CLONTECH) consisted of 50 human-tissue poly(A) RNAs (80–448 ng per dot) normalized by using the RNA expression levels of eight housekeeping genes. Positive controls consisted of human genomic DNA. Negative controls included yeast and Escherichia coli RNA or DNA, as well as human repetitive genomic sequences. The blot was probed with the human prepro-UII cDNA probe and exposed for 2 days onto an X-Omat film ( B ) Northern blot analysis of prepro-UII mRNA expression in the human spinal cord. Spinal cord poly(A) mRNA (2 μg) was hybridized with the human prepro-UII cDNA probe. The molecular weight was determined by using RNA markers. ( C ) X-ray autoradiographs showing the distribution of prepro-UII mRNA in the human spinal cord. Coronal sections were hybridized with the antisense ( Top ) or sense ( Bottom ) prepro-UII riboprobe and exposed for 10 days onto x-ray film.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Cloning of the cDNA encoding the urotensin II precursor in frog and human reveals intense expression of the urotensin II gene in motoneurons of the spinal cord

    doi:

    Figure Lengend Snippet: Tissue distribution of human prepro-UII mRNA. ( A ) Dot blot analysis of prepro-UII mRNA expression in various human tissues. The master blot (CLONTECH) consisted of 50 human-tissue poly(A) RNAs (80–448 ng per dot) normalized by using the RNA expression levels of eight housekeeping genes. Positive controls consisted of human genomic DNA. Negative controls included yeast and Escherichia coli RNA or DNA, as well as human repetitive genomic sequences. The blot was probed with the human prepro-UII cDNA probe and exposed for 2 days onto an X-Omat film ( B ) Northern blot analysis of prepro-UII mRNA expression in the human spinal cord. Spinal cord poly(A) mRNA (2 μg) was hybridized with the human prepro-UII cDNA probe. The molecular weight was determined by using RNA markers. ( C ) X-ray autoradiographs showing the distribution of prepro-UII mRNA in the human spinal cord. Coronal sections were hybridized with the antisense ( Top ) or sense ( Bottom ) prepro-UII riboprobe and exposed for 10 days onto x-ray film.

    Article Snippet: Total RNA (5 μg) from each frog tissue sample was converted to single-stranded cDNA (SuperScript II, Life Technologies, Eragny, France) with oligo (dT)12–18 primer (25 μg/ml) and was analyzed by PCR with the primers 5′-TCCTTTCGGTCGTTGCCCATCATCG-3′ and 5′-CGGTCAGTAGATGGAGCTGGTAATCA-3′.

    Techniques: Dot Blot, Expressing, RNA Expression, Genomic Sequencing, Northern Blot, Molecular Weight

    Tissue distribution of frog prepro-UII mRNA. ( A ) Northern blot analysis of prepro-UII mRNA expression in the frog central nervous system. Total RNA (30 μg) was electrophoresed on a formaldehyde–agarose gel, transferred onto a nylon membrane and probed with the frog prepro-UII cDNA probe. ( B ) Analysis of prepro-UII mRNA distribution in various frog tissues by RT-PCR. Total RNA (5 μg) was reverse transcribed, amplified with prepro-UII specific primers, electrophoresed on an agarose gel and stained with ethidium bromide ( Upper ). The gel was then blotted and hybridized with an internal prepro-UII primer ( Lower ). The control lane contained no DNA. The sizes of the PCR products were evaluated by using a DNA mass ladder (M).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Cloning of the cDNA encoding the urotensin II precursor in frog and human reveals intense expression of the urotensin II gene in motoneurons of the spinal cord

    doi:

    Figure Lengend Snippet: Tissue distribution of frog prepro-UII mRNA. ( A ) Northern blot analysis of prepro-UII mRNA expression in the frog central nervous system. Total RNA (30 μg) was electrophoresed on a formaldehyde–agarose gel, transferred onto a nylon membrane and probed with the frog prepro-UII cDNA probe. ( B ) Analysis of prepro-UII mRNA distribution in various frog tissues by RT-PCR. Total RNA (5 μg) was reverse transcribed, amplified with prepro-UII specific primers, electrophoresed on an agarose gel and stained with ethidium bromide ( Upper ). The gel was then blotted and hybridized with an internal prepro-UII primer ( Lower ). The control lane contained no DNA. The sizes of the PCR products were evaluated by using a DNA mass ladder (M).

    Article Snippet: Total RNA (5 μg) from each frog tissue sample was converted to single-stranded cDNA (SuperScript II, Life Technologies, Eragny, France) with oligo (dT)12–18 primer (25 μg/ml) and was analyzed by PCR with the primers 5′-TCCTTTCGGTCGTTGCCCATCATCG-3′ and 5′-CGGTCAGTAGATGGAGCTGGTAATCA-3′.

    Techniques: Northern Blot, Expressing, Agarose Gel Electrophoresis, Reverse Transcription Polymerase Chain Reaction, Amplification, Staining, Polymerase Chain Reaction