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    Cell Signaling Technology Inc simplechip enzymatic chromatin ip kit
    EGR1 transcription factor binds within the UL135 gene region. (A) Fibroblasts were transduced with EGR1 3xFlag lentivirus and then infected with WT or UL133/8 null mutant (negative control; NC) TB40/E virus (MOI = 1). Chromatin was immunoprecipitated (ChIP) with IgG control or antibodies specific to EGR1 or histone 3 (H3) and the presence of Site 1 or Site 2 was detected in the precipitates by PCR. As a positive control, PCR was also performed on 2% of the ChIP input. Gel is a representative experiment from 3 replicates. Diagram represents the amplicon region used for Site 1 and Site 2 detection. (B) ChIP-qPCR using <t>SimpleChIP</t> Enzymatic Chromatin IP Kit (Cell Signaling) was performed on fibroblasts infected for 48 h and pulsed with EGF for 1h, fibroblasts expressing EGR1 3xFlag infected for 48 h, or pure population of infect CD34 + HPCs in long-term culture for 5 days (6 dpi total). Fibroblasts were infected at an MOI of 1 and the CD34 + HPCs were infected at an MOI of 2. The presence of EGR1 Site 1 or Site 2 sequence was quantified by qPCR relative to a 2% input control and normalized to WT levels.
    Simplechip Enzymatic Chromatin Ip Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 2766 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/simplechip enzymatic chromatin ip kit/product/Cell Signaling Technology Inc
    Average 99 stars, based on 2766 article reviews
    Price from $9.99 to $1999.99
    simplechip enzymatic chromatin ip kit - by Bioz Stars, 2020-09
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    99
    Cell Signaling Technology Inc simple chip plus enzymatic chromatin ip kit
    TIP60 complex inhibits activity of the HBV precore/core promoter. (A) HepG2/NTCP-myc cells were transfected with the indicated reporter plasmid along with pTK-RLuc. At 24 h after transfection, cells were transfected with siControl or siTIP60. Two days after transfection, the level of luciferase activity was measured. Firefly luciferase activity was normalized to Renilla luciferase activity. (B) HepG2/NTCP-myc cells were transfected with pPrecore/core-Luc and pTK-RLuc. At 24 h after transfection, cells were transfected with the indicated siRNA. Two days after transfection, the level of luciferase activity was measured. Firefly luciferase activity was normalized to Renilla luciferase activity. Results are the means ± SD from three independent experiments. (C) Position of the primer pairs (blue line) used for ChIP-qPCR. Red line, HBV cccDNA; open square, enhancer I; closed square, enhancer II; closed circle, basal core promoter; arrow, open reading frame. (D) HepG2/NTCP-myc cells were infected with wild-type HBV in the presence of 4% PEG 8000 and 2% DMSO. At 24 h after infection, cells were infected with a lentiviral vector encoding TIP60. Seven days after infection, cells were cross-linked with a 1% formaldehyde solution for 10 min at room temperature, and the reaction was stopped by the addition of glycine buffer. ChIP assay was performed using the <t>SimpleChIP</t> plus enzymatic chromatin IP kit. (E) HepG2/NTCP-myc cells were transfected with siControl or siTIP60. At 24 h after transfection, cells were infected with wild-type HBV in the presence of 4% PEG 8000 and 2% DMSO. Seven days after infection, cells were cross-linked with a 1% formaldehyde solution for 10 min at room temperature, and the reaction was stopped by the addition of a glycine buffer. ChIP assay was performed using the SimpleChIP plus enzymatic chromatin IP kit.
    Simple Chip Plus Enzymatic Chromatin Ip Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/simple chip plus enzymatic chromatin ip kit/product/Cell Signaling Technology Inc
    Average 99 stars, based on 144 article reviews
    Price from $9.99 to $1999.99
    simple chip plus enzymatic chromatin ip kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc simplechip plus enzymatic chromatin ip kits
    TIP60 complex inhibits activity of the HBV precore/core promoter. (A) HepG2/NTCP-myc cells were transfected with the indicated reporter plasmid along with pTK-RLuc. At 24 h after transfection, cells were transfected with siControl or siTIP60. Two days after transfection, the level of luciferase activity was measured. Firefly luciferase activity was normalized to Renilla luciferase activity. (B) HepG2/NTCP-myc cells were transfected with pPrecore/core-Luc and pTK-RLuc. At 24 h after transfection, cells were transfected with the indicated siRNA. Two days after transfection, the level of luciferase activity was measured. Firefly luciferase activity was normalized to Renilla luciferase activity. Results are the means ± SD from three independent experiments. (C) Position of the primer pairs (blue line) used for ChIP-qPCR. Red line, HBV cccDNA; open square, enhancer I; closed square, enhancer II; closed circle, basal core promoter; arrow, open reading frame. (D) HepG2/NTCP-myc cells were infected with wild-type HBV in the presence of 4% PEG 8000 and 2% DMSO. At 24 h after infection, cells were infected with a lentiviral vector encoding TIP60. Seven days after infection, cells were cross-linked with a 1% formaldehyde solution for 10 min at room temperature, and the reaction was stopped by the addition of glycine buffer. ChIP assay was performed using the <t>SimpleChIP</t> plus enzymatic chromatin IP kit. (E) HepG2/NTCP-myc cells were transfected with siControl or siTIP60. At 24 h after transfection, cells were infected with wild-type HBV in the presence of 4% PEG 8000 and 2% DMSO. Seven days after infection, cells were cross-linked with a 1% formaldehyde solution for 10 min at room temperature, and the reaction was stopped by the addition of a glycine buffer. ChIP assay was performed using the SimpleChIP plus enzymatic chromatin IP kit.
    Simplechip Plus Enzymatic Chromatin Ip Kits, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/simplechip plus enzymatic chromatin ip kits/product/Cell Signaling Technology Inc
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    simplechip plus enzymatic chromatin ip kits - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    91
    Cell Signaling Technology Inc zero blunt topo pcr cloning kit for sequencing
    TIP60 complex inhibits activity of the HBV precore/core promoter. (A) HepG2/NTCP-myc cells were transfected with the indicated reporter plasmid along with pTK-RLuc. At 24 h after transfection, cells were transfected with siControl or siTIP60. Two days after transfection, the level of luciferase activity was measured. Firefly luciferase activity was normalized to Renilla luciferase activity. (B) HepG2/NTCP-myc cells were transfected with pPrecore/core-Luc and pTK-RLuc. At 24 h after transfection, cells were transfected with the indicated siRNA. Two days after transfection, the level of luciferase activity was measured. Firefly luciferase activity was normalized to Renilla luciferase activity. Results are the means ± SD from three independent experiments. (C) Position of the primer pairs (blue line) used for ChIP-qPCR. Red line, HBV cccDNA; open square, enhancer I; closed square, enhancer II; closed circle, basal core promoter; arrow, open reading frame. (D) HepG2/NTCP-myc cells were infected with wild-type HBV in the presence of 4% PEG 8000 and 2% DMSO. At 24 h after infection, cells were infected with a lentiviral vector encoding TIP60. Seven days after infection, cells were cross-linked with a 1% formaldehyde solution for 10 min at room temperature, and the reaction was stopped by the addition of glycine buffer. ChIP assay was performed using the <t>SimpleChIP</t> plus enzymatic chromatin IP kit. (E) HepG2/NTCP-myc cells were transfected with siControl or siTIP60. At 24 h after transfection, cells were infected with wild-type HBV in the presence of 4% PEG 8000 and 2% DMSO. Seven days after infection, cells were cross-linked with a 1% formaldehyde solution for 10 min at room temperature, and the reaction was stopped by the addition of a glycine buffer. ChIP assay was performed using the SimpleChIP plus enzymatic chromatin IP kit.
    Zero Blunt Topo Pcr Cloning Kit For Sequencing, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zero blunt topo pcr cloning kit for sequencing/product/Cell Signaling Technology Inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zero blunt topo pcr cloning kit for sequencing - by Bioz Stars, 2020-09
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    Image Search Results


    EGR1 transcription factor binds within the UL135 gene region. (A) Fibroblasts were transduced with EGR1 3xFlag lentivirus and then infected with WT or UL133/8 null mutant (negative control; NC) TB40/E virus (MOI = 1). Chromatin was immunoprecipitated (ChIP) with IgG control or antibodies specific to EGR1 or histone 3 (H3) and the presence of Site 1 or Site 2 was detected in the precipitates by PCR. As a positive control, PCR was also performed on 2% of the ChIP input. Gel is a representative experiment from 3 replicates. Diagram represents the amplicon region used for Site 1 and Site 2 detection. (B) ChIP-qPCR using SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling) was performed on fibroblasts infected for 48 h and pulsed with EGF for 1h, fibroblasts expressing EGR1 3xFlag infected for 48 h, or pure population of infect CD34 + HPCs in long-term culture for 5 days (6 dpi total). Fibroblasts were infected at an MOI of 1 and the CD34 + HPCs were infected at an MOI of 2. The presence of EGR1 Site 1 or Site 2 sequence was quantified by qPCR relative to a 2% input control and normalized to WT levels.

    Journal: PLoS Pathogens

    Article Title: Host signaling and EGR1 transcriptional control of human cytomegalovirus replication and latencyHCMV miR-US22 down-regulation of EGR-1 regulates CD34+ hematopoietic progenitor cell proliferation and viral reactivation

    doi: 10.1371/journal.ppat.1008037

    Figure Lengend Snippet: EGR1 transcription factor binds within the UL135 gene region. (A) Fibroblasts were transduced with EGR1 3xFlag lentivirus and then infected with WT or UL133/8 null mutant (negative control; NC) TB40/E virus (MOI = 1). Chromatin was immunoprecipitated (ChIP) with IgG control or antibodies specific to EGR1 or histone 3 (H3) and the presence of Site 1 or Site 2 was detected in the precipitates by PCR. As a positive control, PCR was also performed on 2% of the ChIP input. Gel is a representative experiment from 3 replicates. Diagram represents the amplicon region used for Site 1 and Site 2 detection. (B) ChIP-qPCR using SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling) was performed on fibroblasts infected for 48 h and pulsed with EGF for 1h, fibroblasts expressing EGR1 3xFlag infected for 48 h, or pure population of infect CD34 + HPCs in long-term culture for 5 days (6 dpi total). Fibroblasts were infected at an MOI of 1 and the CD34 + HPCs were infected at an MOI of 2. The presence of EGR1 Site 1 or Site 2 sequence was quantified by qPCR relative to a 2% input control and normalized to WT levels.

    Article Snippet: All samples were then processed for ChIP-qPCR using SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling Technologies) as per manufacturer’s recommended protocol.

    Techniques: Transduction, Infection, Mutagenesis, Negative Control, Immunoprecipitation, Chromatin Immunoprecipitation, Polymerase Chain Reaction, Positive Control, Amplification, Real-time Polymerase Chain Reaction, Expressing, Sequencing

    TIP60 complex inhibits activity of the HBV precore/core promoter. (A) HepG2/NTCP-myc cells were transfected with the indicated reporter plasmid along with pTK-RLuc. At 24 h after transfection, cells were transfected with siControl or siTIP60. Two days after transfection, the level of luciferase activity was measured. Firefly luciferase activity was normalized to Renilla luciferase activity. (B) HepG2/NTCP-myc cells were transfected with pPrecore/core-Luc and pTK-RLuc. At 24 h after transfection, cells were transfected with the indicated siRNA. Two days after transfection, the level of luciferase activity was measured. Firefly luciferase activity was normalized to Renilla luciferase activity. Results are the means ± SD from three independent experiments. (C) Position of the primer pairs (blue line) used for ChIP-qPCR. Red line, HBV cccDNA; open square, enhancer I; closed square, enhancer II; closed circle, basal core promoter; arrow, open reading frame. (D) HepG2/NTCP-myc cells were infected with wild-type HBV in the presence of 4% PEG 8000 and 2% DMSO. At 24 h after infection, cells were infected with a lentiviral vector encoding TIP60. Seven days after infection, cells were cross-linked with a 1% formaldehyde solution for 10 min at room temperature, and the reaction was stopped by the addition of glycine buffer. ChIP assay was performed using the SimpleChIP plus enzymatic chromatin IP kit. (E) HepG2/NTCP-myc cells were transfected with siControl or siTIP60. At 24 h after transfection, cells were infected with wild-type HBV in the presence of 4% PEG 8000 and 2% DMSO. Seven days after infection, cells were cross-linked with a 1% formaldehyde solution for 10 min at room temperature, and the reaction was stopped by the addition of a glycine buffer. ChIP assay was performed using the SimpleChIP plus enzymatic chromatin IP kit.

    Journal: Journal of Virology

    Article Title: TIP60 Complex Inhibits Hepatitis B Virus Transcription

    doi: 10.1128/JVI.01788-17

    Figure Lengend Snippet: TIP60 complex inhibits activity of the HBV precore/core promoter. (A) HepG2/NTCP-myc cells were transfected with the indicated reporter plasmid along with pTK-RLuc. At 24 h after transfection, cells were transfected with siControl or siTIP60. Two days after transfection, the level of luciferase activity was measured. Firefly luciferase activity was normalized to Renilla luciferase activity. (B) HepG2/NTCP-myc cells were transfected with pPrecore/core-Luc and pTK-RLuc. At 24 h after transfection, cells were transfected with the indicated siRNA. Two days after transfection, the level of luciferase activity was measured. Firefly luciferase activity was normalized to Renilla luciferase activity. Results are the means ± SD from three independent experiments. (C) Position of the primer pairs (blue line) used for ChIP-qPCR. Red line, HBV cccDNA; open square, enhancer I; closed square, enhancer II; closed circle, basal core promoter; arrow, open reading frame. (D) HepG2/NTCP-myc cells were infected with wild-type HBV in the presence of 4% PEG 8000 and 2% DMSO. At 24 h after infection, cells were infected with a lentiviral vector encoding TIP60. Seven days after infection, cells were cross-linked with a 1% formaldehyde solution for 10 min at room temperature, and the reaction was stopped by the addition of glycine buffer. ChIP assay was performed using the SimpleChIP plus enzymatic chromatin IP kit. (E) HepG2/NTCP-myc cells were transfected with siControl or siTIP60. At 24 h after transfection, cells were infected with wild-type HBV in the presence of 4% PEG 8000 and 2% DMSO. Seven days after infection, cells were cross-linked with a 1% formaldehyde solution for 10 min at room temperature, and the reaction was stopped by the addition of a glycine buffer. ChIP assay was performed using the SimpleChIP plus enzymatic chromatin IP kit.

    Article Snippet: ChIP assay was performed using a SimpleChIP plus enzymatic chromatin IP kit according to the manufacturer's instructions (Cell Signaling Technology).

    Techniques: Activity Assay, Transfection, Plasmid Preparation, Luciferase, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction, Infection