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  • 94
    Merck & Co silica gel 60 f254
    Characterization of a M. smegmatis mptB ( MSMEG3120 ) mutant. A. Map of the MSMEG3120 region in the wild type, parental strain M. smegmatis mc 2 155 and its corresponding region in the Δ MSMEG3120 mutant. res , resolvase site; hyg , hygromycin-resistance gene from Streptomyces hygroscopicus ; sacB , sucrose counterselectable gene from Bacillus subtilis . B. 2D-TLC analysis of the [ 14 C]-labelled (50 000 c.p.m.) polar lipids fraction from M. smegmatis (WT) and M. smegmatis Δ MSMEG3120 strains. The polar lipid extract was examined on aluminum-backed plates of silica <t>gel</t> 60 <t>F254</t> (Merck 5554), using CHCl 3 /CH 3 OH/H 2 O (60:30:6, v/v/v) in the first direction and CHCl 3 /CH 3 COOH/CH 3 OH/H 2 O (40:25:3:6, v/v/v/v) in the second direction. Lipids were visualized by autoradiography by overnight exposure of Kodak X-Omat AR film to the TLC plates to reveal labelled lipids. C. Lipoglycan analysis of wild-type M. smegmatis and M. smegmatis Δ MSMEG3120 using SDS-PAGE and visualized using a Pro-Q emerald glycoprotein stain (Invitrogen). The four major bands represent glycoproteins of 180, 82, 42 and18 kDa respectively.
    Silica Gel 60 F254, supplied by Merck & Co, used in various techniques. Bioz Stars score: 94/100, based on 2557 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA silica gel 60 f254
    Characterization of a M. smegmatis mptB ( MSMEG3120 ) mutant. A. Map of the MSMEG3120 region in the wild type, parental strain M. smegmatis mc 2 155 and its corresponding region in the Δ MSMEG3120 mutant. res , resolvase site; hyg , hygromycin-resistance gene from Streptomyces hygroscopicus ; sacB , sucrose counterselectable gene from Bacillus subtilis . B. 2D-TLC analysis of the [ 14 C]-labelled (50 000 c.p.m.) polar lipids fraction from M. smegmatis (WT) and M. smegmatis Δ MSMEG3120 strains. The polar lipid extract was examined on aluminum-backed plates of silica <t>gel</t> 60 <t>F254</t> (Merck 5554), using CHCl 3 /CH 3 OH/H 2 O (60:30:6, v/v/v) in the first direction and CHCl 3 /CH 3 COOH/CH 3 OH/H 2 O (40:25:3:6, v/v/v/v) in the second direction. Lipids were visualized by autoradiography by overnight exposure of Kodak X-Omat AR film to the TLC plates to reveal labelled lipids. C. Lipoglycan analysis of wild-type M. smegmatis and M. smegmatis Δ MSMEG3120 using SDS-PAGE and visualized using a Pro-Q emerald glycoprotein stain (Invitrogen). The four major bands represent glycoproteins of 180, 82, 42 and18 kDa respectively.
    Silica Gel 60 F254, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 94/100, based on 1655 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Avantor silica gel 60 f254
    Characterization of a M. smegmatis mptB ( MSMEG3120 ) mutant. A. Map of the MSMEG3120 region in the wild type, parental strain M. smegmatis mc 2 155 and its corresponding region in the Δ MSMEG3120 mutant. res , resolvase site; hyg , hygromycin-resistance gene from Streptomyces hygroscopicus ; sacB , sucrose counterselectable gene from Bacillus subtilis . B. 2D-TLC analysis of the [ 14 C]-labelled (50 000 c.p.m.) polar lipids fraction from M. smegmatis (WT) and M. smegmatis Δ MSMEG3120 strains. The polar lipid extract was examined on aluminum-backed plates of silica <t>gel</t> 60 <t>F254</t> (Merck 5554), using CHCl 3 /CH 3 OH/H 2 O (60:30:6, v/v/v) in the first direction and CHCl 3 /CH 3 COOH/CH 3 OH/H 2 O (40:25:3:6, v/v/v/v) in the second direction. Lipids were visualized by autoradiography by overnight exposure of Kodak X-Omat AR film to the TLC plates to reveal labelled lipids. C. Lipoglycan analysis of wild-type M. smegmatis and M. smegmatis Δ MSMEG3120 using SDS-PAGE and visualized using a Pro-Q emerald glycoprotein stain (Invitrogen). The four major bands represent glycoproteins of 180, 82, 42 and18 kDa respectively.
    Silica Gel 60 F254, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    EM Science Inc silica gel 60 f254
    Characterization of a M. smegmatis mptB ( MSMEG3120 ) mutant. A. Map of the MSMEG3120 region in the wild type, parental strain M. smegmatis mc 2 155 and its corresponding region in the Δ MSMEG3120 mutant. res , resolvase site; hyg , hygromycin-resistance gene from Streptomyces hygroscopicus ; sacB , sucrose counterselectable gene from Bacillus subtilis . B. 2D-TLC analysis of the [ 14 C]-labelled (50 000 c.p.m.) polar lipids fraction from M. smegmatis (WT) and M. smegmatis Δ MSMEG3120 strains. The polar lipid extract was examined on aluminum-backed plates of silica <t>gel</t> 60 <t>F254</t> (Merck 5554), using CHCl 3 /CH 3 OH/H 2 O (60:30:6, v/v/v) in the first direction and CHCl 3 /CH 3 COOH/CH 3 OH/H 2 O (40:25:3:6, v/v/v/v) in the second direction. Lipids were visualized by autoradiography by overnight exposure of Kodak X-Omat AR film to the TLC plates to reveal labelled lipids. C. Lipoglycan analysis of wild-type M. smegmatis and M. smegmatis Δ MSMEG3120 using SDS-PAGE and visualized using a Pro-Q emerald glycoprotein stain (Invitrogen). The four major bands represent glycoproteins of 180, 82, 42 and18 kDa respectively.
    Silica Gel 60 F254, supplied by EM Science Inc, used in various techniques. Bioz Stars score: 90/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MACHEREY NAGEL silica gel 60 f254
    Characterization of a M. smegmatis mptB ( MSMEG3120 ) mutant. A. Map of the MSMEG3120 region in the wild type, parental strain M. smegmatis mc 2 155 and its corresponding region in the Δ MSMEG3120 mutant. res , resolvase site; hyg , hygromycin-resistance gene from Streptomyces hygroscopicus ; sacB , sucrose counterselectable gene from Bacillus subtilis . B. 2D-TLC analysis of the [ 14 C]-labelled (50 000 c.p.m.) polar lipids fraction from M. smegmatis (WT) and M. smegmatis Δ MSMEG3120 strains. The polar lipid extract was examined on aluminum-backed plates of silica <t>gel</t> 60 <t>F254</t> (Merck 5554), using CHCl 3 /CH 3 OH/H 2 O (60:30:6, v/v/v) in the first direction and CHCl 3 /CH 3 COOH/CH 3 OH/H 2 O (40:25:3:6, v/v/v/v) in the second direction. Lipids were visualized by autoradiography by overnight exposure of Kodak X-Omat AR film to the TLC plates to reveal labelled lipids. C. Lipoglycan analysis of wild-type M. smegmatis and M. smegmatis Δ MSMEG3120 using SDS-PAGE and visualized using a Pro-Q emerald glycoprotein stain (Invitrogen). The four major bands represent glycoproteins of 180, 82, 42 and18 kDa respectively.
    Silica Gel 60 F254, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 93/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA silica gel 60 f254 silica plates
    Characterization of a M. smegmatis mptB ( MSMEG3120 ) mutant. A. Map of the MSMEG3120 region in the wild type, parental strain M. smegmatis mc 2 155 and its corresponding region in the Δ MSMEG3120 mutant. res , resolvase site; hyg , hygromycin-resistance gene from Streptomyces hygroscopicus ; sacB , sucrose counterselectable gene from Bacillus subtilis . B. 2D-TLC analysis of the [ 14 C]-labelled (50 000 c.p.m.) polar lipids fraction from M. smegmatis (WT) and M. smegmatis Δ MSMEG3120 strains. The polar lipid extract was examined on aluminum-backed plates of silica <t>gel</t> 60 <t>F254</t> (Merck 5554), using CHCl 3 /CH 3 OH/H 2 O (60:30:6, v/v/v) in the first direction and CHCl 3 /CH 3 COOH/CH 3 OH/H 2 O (40:25:3:6, v/v/v/v) in the second direction. Lipids were visualized by autoradiography by overnight exposure of Kodak X-Omat AR film to the TLC plates to reveal labelled lipids. C. Lipoglycan analysis of wild-type M. smegmatis and M. smegmatis Δ MSMEG3120 using SDS-PAGE and visualized using a Pro-Q emerald glycoprotein stain (Invitrogen). The four major bands represent glycoproteins of 180, 82, 42 and18 kDa respectively.
    Silica Gel 60 F254 Silica Plates, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    SiliCycle silica gel 60 f254
    Characterization of a M. smegmatis mptB ( MSMEG3120 ) mutant. A. Map of the MSMEG3120 region in the wild type, parental strain M. smegmatis mc 2 155 and its corresponding region in the Δ MSMEG3120 mutant. res , resolvase site; hyg , hygromycin-resistance gene from Streptomyces hygroscopicus ; sacB , sucrose counterselectable gene from Bacillus subtilis . B. 2D-TLC analysis of the [ 14 C]-labelled (50 000 c.p.m.) polar lipids fraction from M. smegmatis (WT) and M. smegmatis Δ MSMEG3120 strains. The polar lipid extract was examined on aluminum-backed plates of silica <t>gel</t> 60 <t>F254</t> (Merck 5554), using CHCl 3 /CH 3 OH/H 2 O (60:30:6, v/v/v) in the first direction and CHCl 3 /CH 3 COOH/CH 3 OH/H 2 O (40:25:3:6, v/v/v/v) in the second direction. Lipids were visualized by autoradiography by overnight exposure of Kodak X-Omat AR film to the TLC plates to reveal labelled lipids. C. Lipoglycan analysis of wild-type M. smegmatis and M. smegmatis Δ MSMEG3120 using SDS-PAGE and visualized using a Pro-Q emerald glycoprotein stain (Invitrogen). The four major bands represent glycoproteins of 180, 82, 42 and18 kDa respectively.
    Silica Gel 60 F254, supplied by SiliCycle, used in various techniques. Bioz Stars score: 93/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Miles Scientific silica gel 60 f254
    Characterization of a M. smegmatis mptB ( MSMEG3120 ) mutant. A. Map of the MSMEG3120 region in the wild type, parental strain M. smegmatis mc 2 155 and its corresponding region in the Δ MSMEG3120 mutant. res , resolvase site; hyg , hygromycin-resistance gene from Streptomyces hygroscopicus ; sacB , sucrose counterselectable gene from Bacillus subtilis . B. 2D-TLC analysis of the [ 14 C]-labelled (50 000 c.p.m.) polar lipids fraction from M. smegmatis (WT) and M. smegmatis Δ MSMEG3120 strains. The polar lipid extract was examined on aluminum-backed plates of silica <t>gel</t> 60 <t>F254</t> (Merck 5554), using CHCl 3 /CH 3 OH/H 2 O (60:30:6, v/v/v) in the first direction and CHCl 3 /CH 3 COOH/CH 3 OH/H 2 O (40:25:3:6, v/v/v/v) in the second direction. Lipids were visualized by autoradiography by overnight exposure of Kodak X-Omat AR film to the TLC plates to reveal labelled lipids. C. Lipoglycan analysis of wild-type M. smegmatis and M. smegmatis Δ MSMEG3120 using SDS-PAGE and visualized using a Pro-Q emerald glycoprotein stain (Invitrogen). The four major bands represent glycoproteins of 180, 82, 42 and18 kDa respectively.
    Silica Gel 60 F254, supplied by Miles Scientific, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    GE Healthcare silica gel 60 f254
    Characterization of a M. smegmatis mptB ( MSMEG3120 ) mutant. A. Map of the MSMEG3120 region in the wild type, parental strain M. smegmatis mc 2 155 and its corresponding region in the Δ MSMEG3120 mutant. res , resolvase site; hyg , hygromycin-resistance gene from Streptomyces hygroscopicus ; sacB , sucrose counterselectable gene from Bacillus subtilis . B. 2D-TLC analysis of the [ 14 C]-labelled (50 000 c.p.m.) polar lipids fraction from M. smegmatis (WT) and M. smegmatis Δ MSMEG3120 strains. The polar lipid extract was examined on aluminum-backed plates of silica <t>gel</t> 60 <t>F254</t> (Merck 5554), using CHCl 3 /CH 3 OH/H 2 O (60:30:6, v/v/v) in the first direction and CHCl 3 /CH 3 COOH/CH 3 OH/H 2 O (40:25:3:6, v/v/v/v) in the second direction. Lipids were visualized by autoradiography by overnight exposure of Kodak X-Omat AR film to the TLC plates to reveal labelled lipids. C. Lipoglycan analysis of wild-type M. smegmatis and M. smegmatis Δ MSMEG3120 using SDS-PAGE and visualized using a Pro-Q emerald glycoprotein stain (Invitrogen). The four major bands represent glycoproteins of 180, 82, 42 and18 kDa respectively.
    Silica Gel 60 F254, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA silica gel 60 f254 plate
    Characterization of a M. smegmatis mptB ( MSMEG3120 ) mutant. A. Map of the MSMEG3120 region in the wild type, parental strain M. smegmatis mc 2 155 and its corresponding region in the Δ MSMEG3120 mutant. res , resolvase site; hyg , hygromycin-resistance gene from Streptomyces hygroscopicus ; sacB , sucrose counterselectable gene from Bacillus subtilis . B. 2D-TLC analysis of the [ 14 C]-labelled (50 000 c.p.m.) polar lipids fraction from M. smegmatis (WT) and M. smegmatis Δ MSMEG3120 strains. The polar lipid extract was examined on aluminum-backed plates of silica <t>gel</t> 60 <t>F254</t> (Merck 5554), using CHCl 3 /CH 3 OH/H 2 O (60:30:6, v/v/v) in the first direction and CHCl 3 /CH 3 COOH/CH 3 OH/H 2 O (40:25:3:6, v/v/v/v) in the second direction. Lipids were visualized by autoradiography by overnight exposure of Kodak X-Omat AR film to the TLC plates to reveal labelled lipids. C. Lipoglycan analysis of wild-type M. smegmatis and M. smegmatis Δ MSMEG3120 using SDS-PAGE and visualized using a Pro-Q emerald glycoprotein stain (Invitrogen). The four major bands represent glycoproteins of 180, 82, 42 and18 kDa respectively.
    Silica Gel 60 F254 Plate, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 89/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co silica gel 60 f254 plates
    Characterization of a M. smegmatis mptB ( MSMEG3120 ) mutant. A. Map of the MSMEG3120 region in the wild type, parental strain M. smegmatis mc 2 155 and its corresponding region in the Δ MSMEG3120 mutant. res , resolvase site; hyg , hygromycin-resistance gene from Streptomyces hygroscopicus ; sacB , sucrose counterselectable gene from Bacillus subtilis . B. 2D-TLC analysis of the [ 14 C]-labelled (50 000 c.p.m.) polar lipids fraction from M. smegmatis (WT) and M. smegmatis Δ MSMEG3120 strains. The polar lipid extract was examined on aluminum-backed plates of silica <t>gel</t> 60 <t>F254</t> (Merck 5554), using CHCl 3 /CH 3 OH/H 2 O (60:30:6, v/v/v) in the first direction and CHCl 3 /CH 3 COOH/CH 3 OH/H 2 O (40:25:3:6, v/v/v/v) in the second direction. Lipids were visualized by autoradiography by overnight exposure of Kodak X-Omat AR film to the TLC plates to reveal labelled lipids. C. Lipoglycan analysis of wild-type M. smegmatis and M. smegmatis Δ MSMEG3120 using SDS-PAGE and visualized using a Pro-Q emerald glycoprotein stain (Invitrogen). The four major bands represent glycoproteins of 180, 82, 42 and18 kDa respectively.
    Silica Gel 60 F254 Plates, supplied by Merck & Co, used in various techniques. Bioz Stars score: 94/100, based on 1549 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co precoated silica gel 60 f254
    Characterization of a M. smegmatis mptB ( MSMEG3120 ) mutant. A. Map of the MSMEG3120 region in the wild type, parental strain M. smegmatis mc 2 155 and its corresponding region in the Δ MSMEG3120 mutant. res , resolvase site; hyg , hygromycin-resistance gene from Streptomyces hygroscopicus ; sacB , sucrose counterselectable gene from Bacillus subtilis . B. 2D-TLC analysis of the [ 14 C]-labelled (50 000 c.p.m.) polar lipids fraction from M. smegmatis (WT) and M. smegmatis Δ MSMEG3120 strains. The polar lipid extract was examined on aluminum-backed plates of silica <t>gel</t> 60 <t>F254</t> (Merck 5554), using CHCl 3 /CH 3 OH/H 2 O (60:30:6, v/v/v) in the first direction and CHCl 3 /CH 3 COOH/CH 3 OH/H 2 O (40:25:3:6, v/v/v/v) in the second direction. Lipids were visualized by autoradiography by overnight exposure of Kodak X-Omat AR film to the TLC plates to reveal labelled lipids. C. Lipoglycan analysis of wild-type M. smegmatis and M. smegmatis Δ MSMEG3120 using SDS-PAGE and visualized using a Pro-Q emerald glycoprotein stain (Invitrogen). The four major bands represent glycoproteins of 180, 82, 42 and18 kDa respectively.
    Precoated Silica Gel 60 F254, supplied by Merck & Co, used in various techniques. Bioz Stars score: 89/100, based on 194 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    EM Science Inc silica gel 60 f254 plates
    Characterization of a M. smegmatis mptB ( MSMEG3120 ) mutant. A. Map of the MSMEG3120 region in the wild type, parental strain M. smegmatis mc 2 155 and its corresponding region in the Δ MSMEG3120 mutant. res , resolvase site; hyg , hygromycin-resistance gene from Streptomyces hygroscopicus ; sacB , sucrose counterselectable gene from Bacillus subtilis . B. 2D-TLC analysis of the [ 14 C]-labelled (50 000 c.p.m.) polar lipids fraction from M. smegmatis (WT) and M. smegmatis Δ MSMEG3120 strains. The polar lipid extract was examined on aluminum-backed plates of silica <t>gel</t> 60 <t>F254</t> (Merck 5554), using CHCl 3 /CH 3 OH/H 2 O (60:30:6, v/v/v) in the first direction and CHCl 3 /CH 3 COOH/CH 3 OH/H 2 O (40:25:3:6, v/v/v/v) in the second direction. Lipids were visualized by autoradiography by overnight exposure of Kodak X-Omat AR film to the TLC plates to reveal labelled lipids. C. Lipoglycan analysis of wild-type M. smegmatis and M. smegmatis Δ MSMEG3120 using SDS-PAGE and visualized using a Pro-Q emerald glycoprotein stain (Invitrogen). The four major bands represent glycoproteins of 180, 82, 42 and18 kDa respectively.
    Silica Gel 60 F254 Plates, supplied by EM Science Inc, used in various techniques. Bioz Stars score: 92/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MACHEREY NAGEL silica gel 60 f254 plates
    Characterization of a M. smegmatis mptB ( MSMEG3120 ) mutant. A. Map of the MSMEG3120 region in the wild type, parental strain M. smegmatis mc 2 155 and its corresponding region in the Δ MSMEG3120 mutant. res , resolvase site; hyg , hygromycin-resistance gene from Streptomyces hygroscopicus ; sacB , sucrose counterselectable gene from Bacillus subtilis . B. 2D-TLC analysis of the [ 14 C]-labelled (50 000 c.p.m.) polar lipids fraction from M. smegmatis (WT) and M. smegmatis Δ MSMEG3120 strains. The polar lipid extract was examined on aluminum-backed plates of silica <t>gel</t> 60 <t>F254</t> (Merck 5554), using CHCl 3 /CH 3 OH/H 2 O (60:30:6, v/v/v) in the first direction and CHCl 3 /CH 3 COOH/CH 3 OH/H 2 O (40:25:3:6, v/v/v/v) in the second direction. Lipids were visualized by autoradiography by overnight exposure of Kodak X-Omat AR film to the TLC plates to reveal labelled lipids. C. Lipoglycan analysis of wild-type M. smegmatis and M. smegmatis Δ MSMEG3120 using SDS-PAGE and visualized using a Pro-Q emerald glycoprotein stain (Invitrogen). The four major bands represent glycoproteins of 180, 82, 42 and18 kDa respectively.
    Silica Gel 60 F254 Plates, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA fluorescent silica gel 60 f254
    Characterization of a M. smegmatis mptB ( MSMEG3120 ) mutant. A. Map of the MSMEG3120 region in the wild type, parental strain M. smegmatis mc 2 155 and its corresponding region in the Δ MSMEG3120 mutant. res , resolvase site; hyg , hygromycin-resistance gene from Streptomyces hygroscopicus ; sacB , sucrose counterselectable gene from Bacillus subtilis . B. 2D-TLC analysis of the [ 14 C]-labelled (50 000 c.p.m.) polar lipids fraction from M. smegmatis (WT) and M. smegmatis Δ MSMEG3120 strains. The polar lipid extract was examined on aluminum-backed plates of silica <t>gel</t> 60 <t>F254</t> (Merck 5554), using CHCl 3 /CH 3 OH/H 2 O (60:30:6, v/v/v) in the first direction and CHCl 3 /CH 3 COOH/CH 3 OH/H 2 O (40:25:3:6, v/v/v/v) in the second direction. Lipids were visualized by autoradiography by overnight exposure of Kodak X-Omat AR film to the TLC plates to reveal labelled lipids. C. Lipoglycan analysis of wild-type M. smegmatis and M. smegmatis Δ MSMEG3120 using SDS-PAGE and visualized using a Pro-Q emerald glycoprotein stain (Invitrogen). The four major bands represent glycoproteins of 180, 82, 42 and18 kDa respectively.
    Fluorescent Silica Gel 60 F254, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA plc silica gel 60 f254
    Characterization of a M. smegmatis mptB ( MSMEG3120 ) mutant. A. Map of the MSMEG3120 region in the wild type, parental strain M. smegmatis mc 2 155 and its corresponding region in the Δ MSMEG3120 mutant. res , resolvase site; hyg , hygromycin-resistance gene from Streptomyces hygroscopicus ; sacB , sucrose counterselectable gene from Bacillus subtilis . B. 2D-TLC analysis of the [ 14 C]-labelled (50 000 c.p.m.) polar lipids fraction from M. smegmatis (WT) and M. smegmatis Δ MSMEG3120 strains. The polar lipid extract was examined on aluminum-backed plates of silica <t>gel</t> 60 <t>F254</t> (Merck 5554), using CHCl 3 /CH 3 OH/H 2 O (60:30:6, v/v/v) in the first direction and CHCl 3 /CH 3 COOH/CH 3 OH/H 2 O (40:25:3:6, v/v/v/v) in the second direction. Lipids were visualized by autoradiography by overnight exposure of Kodak X-Omat AR film to the TLC plates to reveal labelled lipids. C. Lipoglycan analysis of wild-type M. smegmatis and M. smegmatis Δ MSMEG3120 using SDS-PAGE and visualized using a Pro-Q emerald glycoprotein stain (Invitrogen). The four major bands represent glycoproteins of 180, 82, 42 and18 kDa respectively.
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    Characterization of a M. smegmatis mptB ( MSMEG3120 ) mutant. A. Map of the MSMEG3120 region in the wild type, parental strain M. smegmatis mc 2 155 and its corresponding region in the Δ MSMEG3120 mutant. res , resolvase site; hyg , hygromycin-resistance gene from Streptomyces hygroscopicus ; sacB , sucrose counterselectable gene from Bacillus subtilis . B. 2D-TLC analysis of the [ 14 C]-labelled (50 000 c.p.m.) polar lipids fraction from M. smegmatis (WT) and M. smegmatis Δ MSMEG3120 strains. The polar lipid extract was examined on aluminum-backed plates of silica <t>gel</t> 60 <t>F254</t> (Merck 5554), using CHCl 3 /CH 3 OH/H 2 O (60:30:6, v/v/v) in the first direction and CHCl 3 /CH 3 COOH/CH 3 OH/H 2 O (40:25:3:6, v/v/v/v) in the second direction. Lipids were visualized by autoradiography by overnight exposure of Kodak X-Omat AR film to the TLC plates to reveal labelled lipids. C. Lipoglycan analysis of wild-type M. smegmatis and M. smegmatis Δ MSMEG3120 using SDS-PAGE and visualized using a Pro-Q emerald glycoprotein stain (Invitrogen). The four major bands represent glycoproteins of 180, 82, 42 and18 kDa respectively.
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    Characterization of a M. smegmatis mptB ( MSMEG3120 ) mutant. A. Map of the MSMEG3120 region in the wild type, parental strain M. smegmatis mc 2 155 and its corresponding region in the Δ MSMEG3120 mutant. res , resolvase site; hyg , hygromycin-resistance gene from Streptomyces hygroscopicus ; sacB , sucrose counterselectable gene from Bacillus subtilis . B. 2D-TLC analysis of the [ 14 C]-labelled (50 000 c.p.m.) polar lipids fraction from M. smegmatis (WT) and M. smegmatis Δ MSMEG3120 strains. The polar lipid extract was examined on aluminum-backed plates of silica <t>gel</t> 60 <t>F254</t> (Merck 5554), using CHCl 3 /CH 3 OH/H 2 O (60:30:6, v/v/v) in the first direction and CHCl 3 /CH 3 COOH/CH 3 OH/H 2 O (40:25:3:6, v/v/v/v) in the second direction. Lipids were visualized by autoradiography by overnight exposure of Kodak X-Omat AR film to the TLC plates to reveal labelled lipids. C. Lipoglycan analysis of wild-type M. smegmatis and M. smegmatis Δ MSMEG3120 using SDS-PAGE and visualized using a Pro-Q emerald glycoprotein stain (Invitrogen). The four major bands represent glycoproteins of 180, 82, 42 and18 kDa respectively.
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    Characterization of a M. smegmatis mptB ( MSMEG3120 ) mutant. A. Map of the MSMEG3120 region in the wild type, parental strain M. smegmatis mc 2 155 and its corresponding region in the Δ MSMEG3120 mutant. res , resolvase site; hyg , hygromycin-resistance gene from Streptomyces hygroscopicus ; sacB , sucrose counterselectable gene from Bacillus subtilis . B. 2D-TLC analysis of the [ 14 C]-labelled (50 000 c.p.m.) polar lipids fraction from M. smegmatis (WT) and M. smegmatis Δ MSMEG3120 strains. The polar lipid extract was examined on aluminum-backed plates of silica <t>gel</t> 60 <t>F254</t> (Merck 5554), using CHCl 3 /CH 3 OH/H 2 O (60:30:6, v/v/v) in the first direction and CHCl 3 /CH 3 COOH/CH 3 OH/H 2 O (40:25:3:6, v/v/v/v) in the second direction. Lipids were visualized by autoradiography by overnight exposure of Kodak X-Omat AR film to the TLC plates to reveal labelled lipids. C. Lipoglycan analysis of wild-type M. smegmatis and M. smegmatis Δ MSMEG3120 using SDS-PAGE and visualized using a Pro-Q emerald glycoprotein stain (Invitrogen). The four major bands represent glycoproteins of 180, 82, 42 and18 kDa respectively.
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    Characterization of a M. smegmatis mptB ( MSMEG3120 ) mutant. A. Map of the MSMEG3120 region in the wild type, parental strain M. smegmatis mc 2 155 and its corresponding region in the Δ MSMEG3120 mutant. res , resolvase site; hyg , hygromycin-resistance gene from Streptomyces hygroscopicus ; sacB , sucrose counterselectable gene from Bacillus subtilis . B. 2D-TLC analysis of the [ 14 C]-labelled (50 000 c.p.m.) polar lipids fraction from M. smegmatis (WT) and M. smegmatis Δ MSMEG3120 strains. The polar lipid extract was examined on aluminum-backed plates of silica <t>gel</t> 60 <t>F254</t> (Merck 5554), using CHCl 3 /CH 3 OH/H 2 O (60:30:6, v/v/v) in the first direction and CHCl 3 /CH 3 COOH/CH 3 OH/H 2 O (40:25:3:6, v/v/v/v) in the second direction. Lipids were visualized by autoradiography by overnight exposure of Kodak X-Omat AR film to the TLC plates to reveal labelled lipids. C. Lipoglycan analysis of wild-type M. smegmatis and M. smegmatis Δ MSMEG3120 using SDS-PAGE and visualized using a Pro-Q emerald glycoprotein stain (Invitrogen). The four major bands represent glycoproteins of 180, 82, 42 and18 kDa respectively.
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    Image Search Results


    Characterization of a M. smegmatis mptB ( MSMEG3120 ) mutant. A. Map of the MSMEG3120 region in the wild type, parental strain M. smegmatis mc 2 155 and its corresponding region in the Δ MSMEG3120 mutant. res , resolvase site; hyg , hygromycin-resistance gene from Streptomyces hygroscopicus ; sacB , sucrose counterselectable gene from Bacillus subtilis . B. 2D-TLC analysis of the [ 14 C]-labelled (50 000 c.p.m.) polar lipids fraction from M. smegmatis (WT) and M. smegmatis Δ MSMEG3120 strains. The polar lipid extract was examined on aluminum-backed plates of silica gel 60 F254 (Merck 5554), using CHCl 3 /CH 3 OH/H 2 O (60:30:6, v/v/v) in the first direction and CHCl 3 /CH 3 COOH/CH 3 OH/H 2 O (40:25:3:6, v/v/v/v) in the second direction. Lipids were visualized by autoradiography by overnight exposure of Kodak X-Omat AR film to the TLC plates to reveal labelled lipids. C. Lipoglycan analysis of wild-type M. smegmatis and M. smegmatis Δ MSMEG3120 using SDS-PAGE and visualized using a Pro-Q emerald glycoprotein stain (Invitrogen). The four major bands represent glycoproteins of 180, 82, 42 and18 kDa respectively.

    Journal: Molecular Microbiology

    Article Title: Identification of a novel ?(1- > 6) mannopyranosyltransferase MptB from Corynebacterium glutamicum by deletion of a conserved gene, NCgl1505, affords a lipomannan- and lipoarabinomannan-deficient mutant

    doi: 10.1111/j.1365-2958.2008.06265.x

    Figure Lengend Snippet: Characterization of a M. smegmatis mptB ( MSMEG3120 ) mutant. A. Map of the MSMEG3120 region in the wild type, parental strain M. smegmatis mc 2 155 and its corresponding region in the Δ MSMEG3120 mutant. res , resolvase site; hyg , hygromycin-resistance gene from Streptomyces hygroscopicus ; sacB , sucrose counterselectable gene from Bacillus subtilis . B. 2D-TLC analysis of the [ 14 C]-labelled (50 000 c.p.m.) polar lipids fraction from M. smegmatis (WT) and M. smegmatis Δ MSMEG3120 strains. The polar lipid extract was examined on aluminum-backed plates of silica gel 60 F254 (Merck 5554), using CHCl 3 /CH 3 OH/H 2 O (60:30:6, v/v/v) in the first direction and CHCl 3 /CH 3 COOH/CH 3 OH/H 2 O (40:25:3:6, v/v/v/v) in the second direction. Lipids were visualized by autoradiography by overnight exposure of Kodak X-Omat AR film to the TLC plates to reveal labelled lipids. C. Lipoglycan analysis of wild-type M. smegmatis and M. smegmatis Δ MSMEG3120 using SDS-PAGE and visualized using a Pro-Q emerald glycoprotein stain (Invitrogen). The four major bands represent glycoproteins of 180, 82, 42 and18 kDa respectively.

    Article Snippet: The polar lipid extract was examined by 2D-TLC on aluminum-backed plates of silica gel 60 F254 (Merck 5554), using CHCl3 /CH3 OH/H2 O (60:30:6, v/v/v) in the first direction and CHCl3 /CH3 COOH/CH3 OH/H2 O (40:25:3:6, v/v/v/v) in the second direction.

    Techniques: Mutagenesis, Thin Layer Chromatography, Autoradiography, SDS Page, Staining