shrimp alkaline phosphatase Takara Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs exonuclease i
    Exonuclease I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 5008 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exonuclease i/product/New England Biolabs
    Average 99 stars, based on 5008 article reviews
    Price from $9.99 to $1999.99
    exonuclease i - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher bigdye terminator v3 1 cycle sequencing kit
    Bigdye Terminator V3 1 Cycle Sequencing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 32287 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bigdye terminator v3 1 cycle sequencing kit/product/Thermo Fisher
    Average 99 stars, based on 32287 article reviews
    Price from $9.99 to $1999.99
    bigdye terminator v3 1 cycle sequencing kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    TaKaRa shrimp alkaline phosphatase
    Identification of phosphorylation sites of mouse HP1α. (A) The phosphorylation patterns of mouse HP1α analyzed by phos-tag Western blotting (phos-tag WB). Whole-cell lysates (WCLs) of NIH 3T3 cells were resolved on an SDS-PAGE gel containing phos-tag acrylamide (phos-tag) or on a standard SDS-PAGE gel (mock). As an unphosphorylated control, <t>SAP-treated</t> <t>WCL</t> was loaded on the same gel. HP1α was detected using an anti-HP1α antibody. (B) Phosphorylation patterns of mouse HP1α in cells arrested at different cell cycle stages. WCLs were prepared from NIH 3T3 cells grown under the indicated conditions and analyzed by phos-tag WB. The arrowhead indicates an HP1α with basal band shift. The asterisk represents an HP1α species with metaphase-associated phosphorylation. The same membrane was immunoblotted with anti-α-tubulin and anti-TIF1β antibodies for loading controls. (C) Amino acid sequence of mouse HP1α. Light- and dark-shaded boxes indicate the CD and CSD, respectively. The sites of serine (S)-to-alanine (A) substitutions introduced in the following experiments are shown in boldface. (D) Phos-tag and standard WB analysis of wild-type and mutant HP1αs. WCLs were prepared from NIH 3T3 cells transiently expressing FLAG-tagged wild-type HP1α or HP1α with N-terminal (N-term: S11-14A) mutations, hinge (Hinge: S92, 93, 95, 97A) mutations, or both (N-term + hinge). Each FLAG-HP1α with an SAP-treated control was analyzed by phos-tag WB using an anti-FLAG antibody. The phosphorylation state of HP1α represented by each protein band was deduced from the distance of migration level of each band (Rf) and is indicated to the right of the phos-tag WB panel. (E) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the N-terminal region. The phosphorylation patterns of the indicated FLAG-HP1α species were analyzed by phos-tag WB using a higher concentration (80 μM) of phos-tag acrylamide. (F) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the hinge region. (G) The numbers of phosphorylated peptides identified by liquid chromatography (LC)-MS/MS analysis of affinity-purified HP1α. (H) Summary of the phosphorylation sites of mouse HP1α deduced from the experiments described above.
    Shrimp Alkaline Phosphatase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 493 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shrimp alkaline phosphatase/product/TaKaRa
    Average 99 stars, based on 493 article reviews
    Price from $9.99 to $1999.99
    shrimp alkaline phosphatase - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    96
    TaKaRa exonuclease i
    Identification of phosphorylation sites of mouse HP1α. (A) The phosphorylation patterns of mouse HP1α analyzed by phos-tag Western blotting (phos-tag WB). Whole-cell lysates (WCLs) of NIH 3T3 cells were resolved on an SDS-PAGE gel containing phos-tag acrylamide (phos-tag) or on a standard SDS-PAGE gel (mock). As an unphosphorylated control, <t>SAP-treated</t> <t>WCL</t> was loaded on the same gel. HP1α was detected using an anti-HP1α antibody. (B) Phosphorylation patterns of mouse HP1α in cells arrested at different cell cycle stages. WCLs were prepared from NIH 3T3 cells grown under the indicated conditions and analyzed by phos-tag WB. The arrowhead indicates an HP1α with basal band shift. The asterisk represents an HP1α species with metaphase-associated phosphorylation. The same membrane was immunoblotted with anti-α-tubulin and anti-TIF1β antibodies for loading controls. (C) Amino acid sequence of mouse HP1α. Light- and dark-shaded boxes indicate the CD and CSD, respectively. The sites of serine (S)-to-alanine (A) substitutions introduced in the following experiments are shown in boldface. (D) Phos-tag and standard WB analysis of wild-type and mutant HP1αs. WCLs were prepared from NIH 3T3 cells transiently expressing FLAG-tagged wild-type HP1α or HP1α with N-terminal (N-term: S11-14A) mutations, hinge (Hinge: S92, 93, 95, 97A) mutations, or both (N-term + hinge). Each FLAG-HP1α with an SAP-treated control was analyzed by phos-tag WB using an anti-FLAG antibody. The phosphorylation state of HP1α represented by each protein band was deduced from the distance of migration level of each band (Rf) and is indicated to the right of the phos-tag WB panel. (E) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the N-terminal region. The phosphorylation patterns of the indicated FLAG-HP1α species were analyzed by phos-tag WB using a higher concentration (80 μM) of phos-tag acrylamide. (F) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the hinge region. (G) The numbers of phosphorylated peptides identified by liquid chromatography (LC)-MS/MS analysis of affinity-purified HP1α. (H) Summary of the phosphorylation sites of mouse HP1α deduced from the experiments described above.
    Exonuclease I, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 570 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exonuclease i/product/TaKaRa
    Average 96 stars, based on 570 article reviews
    Price from $9.99 to $1999.99
    exonuclease i - by Bioz Stars, 2020-09
    96/100 stars
      Buy from Supplier

    94
    GE Healthcare shrimp alkaline phosphatase
    Identification of phosphorylation sites of mouse HP1α. (A) The phosphorylation patterns of mouse HP1α analyzed by phos-tag Western blotting (phos-tag WB). Whole-cell lysates (WCLs) of NIH 3T3 cells were resolved on an SDS-PAGE gel containing phos-tag acrylamide (phos-tag) or on a standard SDS-PAGE gel (mock). As an unphosphorylated control, <t>SAP-treated</t> <t>WCL</t> was loaded on the same gel. HP1α was detected using an anti-HP1α antibody. (B) Phosphorylation patterns of mouse HP1α in cells arrested at different cell cycle stages. WCLs were prepared from NIH 3T3 cells grown under the indicated conditions and analyzed by phos-tag WB. The arrowhead indicates an HP1α with basal band shift. The asterisk represents an HP1α species with metaphase-associated phosphorylation. The same membrane was immunoblotted with anti-α-tubulin and anti-TIF1β antibodies for loading controls. (C) Amino acid sequence of mouse HP1α. Light- and dark-shaded boxes indicate the CD and CSD, respectively. The sites of serine (S)-to-alanine (A) substitutions introduced in the following experiments are shown in boldface. (D) Phos-tag and standard WB analysis of wild-type and mutant HP1αs. WCLs were prepared from NIH 3T3 cells transiently expressing FLAG-tagged wild-type HP1α or HP1α with N-terminal (N-term: S11-14A) mutations, hinge (Hinge: S92, 93, 95, 97A) mutations, or both (N-term + hinge). Each FLAG-HP1α with an SAP-treated control was analyzed by phos-tag WB using an anti-FLAG antibody. The phosphorylation state of HP1α represented by each protein band was deduced from the distance of migration level of each band (Rf) and is indicated to the right of the phos-tag WB panel. (E) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the N-terminal region. The phosphorylation patterns of the indicated FLAG-HP1α species were analyzed by phos-tag WB using a higher concentration (80 μM) of phos-tag acrylamide. (F) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the hinge region. (G) The numbers of phosphorylated peptides identified by liquid chromatography (LC)-MS/MS analysis of affinity-purified HP1α. (H) Summary of the phosphorylation sites of mouse HP1α deduced from the experiments described above.
    Shrimp Alkaline Phosphatase, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 1683 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shrimp alkaline phosphatase/product/GE Healthcare
    Average 94 stars, based on 1683 article reviews
    Price from $9.99 to $1999.99
    shrimp alkaline phosphatase - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    99
    Thermo Fisher exonuclease i
    Identification of phosphorylation sites of mouse HP1α. (A) The phosphorylation patterns of mouse HP1α analyzed by phos-tag Western blotting (phos-tag WB). Whole-cell lysates (WCLs) of NIH 3T3 cells were resolved on an SDS-PAGE gel containing phos-tag acrylamide (phos-tag) or on a standard SDS-PAGE gel (mock). As an unphosphorylated control, <t>SAP-treated</t> <t>WCL</t> was loaded on the same gel. HP1α was detected using an anti-HP1α antibody. (B) Phosphorylation patterns of mouse HP1α in cells arrested at different cell cycle stages. WCLs were prepared from NIH 3T3 cells grown under the indicated conditions and analyzed by phos-tag WB. The arrowhead indicates an HP1α with basal band shift. The asterisk represents an HP1α species with metaphase-associated phosphorylation. The same membrane was immunoblotted with anti-α-tubulin and anti-TIF1β antibodies for loading controls. (C) Amino acid sequence of mouse HP1α. Light- and dark-shaded boxes indicate the CD and CSD, respectively. The sites of serine (S)-to-alanine (A) substitutions introduced in the following experiments are shown in boldface. (D) Phos-tag and standard WB analysis of wild-type and mutant HP1αs. WCLs were prepared from NIH 3T3 cells transiently expressing FLAG-tagged wild-type HP1α or HP1α with N-terminal (N-term: S11-14A) mutations, hinge (Hinge: S92, 93, 95, 97A) mutations, or both (N-term + hinge). Each FLAG-HP1α with an SAP-treated control was analyzed by phos-tag WB using an anti-FLAG antibody. The phosphorylation state of HP1α represented by each protein band was deduced from the distance of migration level of each band (Rf) and is indicated to the right of the phos-tag WB panel. (E) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the N-terminal region. The phosphorylation patterns of the indicated FLAG-HP1α species were analyzed by phos-tag WB using a higher concentration (80 μM) of phos-tag acrylamide. (F) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the hinge region. (G) The numbers of phosphorylated peptides identified by liquid chromatography (LC)-MS/MS analysis of affinity-purified HP1α. (H) Summary of the phosphorylation sites of mouse HP1α deduced from the experiments described above.
    Exonuclease I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5308 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exonuclease i/product/Thermo Fisher
    Average 99 stars, based on 5308 article reviews
    Price from $9.99 to $1999.99
    exonuclease i - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    90
    Thermo Fisher abi prism 3730 sequencer
    Identification of phosphorylation sites of mouse HP1α. (A) The phosphorylation patterns of mouse HP1α analyzed by phos-tag Western blotting (phos-tag WB). Whole-cell lysates (WCLs) of NIH 3T3 cells were resolved on an SDS-PAGE gel containing phos-tag acrylamide (phos-tag) or on a standard SDS-PAGE gel (mock). As an unphosphorylated control, <t>SAP-treated</t> <t>WCL</t> was loaded on the same gel. HP1α was detected using an anti-HP1α antibody. (B) Phosphorylation patterns of mouse HP1α in cells arrested at different cell cycle stages. WCLs were prepared from NIH 3T3 cells grown under the indicated conditions and analyzed by phos-tag WB. The arrowhead indicates an HP1α with basal band shift. The asterisk represents an HP1α species with metaphase-associated phosphorylation. The same membrane was immunoblotted with anti-α-tubulin and anti-TIF1β antibodies for loading controls. (C) Amino acid sequence of mouse HP1α. Light- and dark-shaded boxes indicate the CD and CSD, respectively. The sites of serine (S)-to-alanine (A) substitutions introduced in the following experiments are shown in boldface. (D) Phos-tag and standard WB analysis of wild-type and mutant HP1αs. WCLs were prepared from NIH 3T3 cells transiently expressing FLAG-tagged wild-type HP1α or HP1α with N-terminal (N-term: S11-14A) mutations, hinge (Hinge: S92, 93, 95, 97A) mutations, or both (N-term + hinge). Each FLAG-HP1α with an SAP-treated control was analyzed by phos-tag WB using an anti-FLAG antibody. The phosphorylation state of HP1α represented by each protein band was deduced from the distance of migration level of each band (Rf) and is indicated to the right of the phos-tag WB panel. (E) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the N-terminal region. The phosphorylation patterns of the indicated FLAG-HP1α species were analyzed by phos-tag WB using a higher concentration (80 μM) of phos-tag acrylamide. (F) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the hinge region. (G) The numbers of phosphorylated peptides identified by liquid chromatography (LC)-MS/MS analysis of affinity-purified HP1α. (H) Summary of the phosphorylation sites of mouse HP1α deduced from the experiments described above.
    Abi Prism 3730 Sequencer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 567 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi prism 3730 sequencer/product/Thermo Fisher
    Average 90 stars, based on 567 article reviews
    Price from $9.99 to $1999.99
    abi prism 3730 sequencer - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    99
    TaKaRa dntps
    Identification of phosphorylation sites of mouse HP1α. (A) The phosphorylation patterns of mouse HP1α analyzed by phos-tag Western blotting (phos-tag WB). Whole-cell lysates (WCLs) of NIH 3T3 cells were resolved on an SDS-PAGE gel containing phos-tag acrylamide (phos-tag) or on a standard SDS-PAGE gel (mock). As an unphosphorylated control, <t>SAP-treated</t> <t>WCL</t> was loaded on the same gel. HP1α was detected using an anti-HP1α antibody. (B) Phosphorylation patterns of mouse HP1α in cells arrested at different cell cycle stages. WCLs were prepared from NIH 3T3 cells grown under the indicated conditions and analyzed by phos-tag WB. The arrowhead indicates an HP1α with basal band shift. The asterisk represents an HP1α species with metaphase-associated phosphorylation. The same membrane was immunoblotted with anti-α-tubulin and anti-TIF1β antibodies for loading controls. (C) Amino acid sequence of mouse HP1α. Light- and dark-shaded boxes indicate the CD and CSD, respectively. The sites of serine (S)-to-alanine (A) substitutions introduced in the following experiments are shown in boldface. (D) Phos-tag and standard WB analysis of wild-type and mutant HP1αs. WCLs were prepared from NIH 3T3 cells transiently expressing FLAG-tagged wild-type HP1α or HP1α with N-terminal (N-term: S11-14A) mutations, hinge (Hinge: S92, 93, 95, 97A) mutations, or both (N-term + hinge). Each FLAG-HP1α with an SAP-treated control was analyzed by phos-tag WB using an anti-FLAG antibody. The phosphorylation state of HP1α represented by each protein band was deduced from the distance of migration level of each band (Rf) and is indicated to the right of the phos-tag WB panel. (E) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the N-terminal region. The phosphorylation patterns of the indicated FLAG-HP1α species were analyzed by phos-tag WB using a higher concentration (80 μM) of phos-tag acrylamide. (F) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the hinge region. (G) The numbers of phosphorylated peptides identified by liquid chromatography (LC)-MS/MS analysis of affinity-purified HP1α. (H) Summary of the phosphorylation sites of mouse HP1α deduced from the experiments described above.
    Dntps, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 14384 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntps/product/TaKaRa
    Average 99 stars, based on 14384 article reviews
    Price from $9.99 to $1999.99
    dntps - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher exosap it
    Identification of phosphorylation sites of mouse HP1α. (A) The phosphorylation patterns of mouse HP1α analyzed by phos-tag Western blotting (phos-tag WB). Whole-cell lysates (WCLs) of NIH 3T3 cells were resolved on an SDS-PAGE gel containing phos-tag acrylamide (phos-tag) or on a standard SDS-PAGE gel (mock). As an unphosphorylated control, <t>SAP-treated</t> <t>WCL</t> was loaded on the same gel. HP1α was detected using an anti-HP1α antibody. (B) Phosphorylation patterns of mouse HP1α in cells arrested at different cell cycle stages. WCLs were prepared from NIH 3T3 cells grown under the indicated conditions and analyzed by phos-tag WB. The arrowhead indicates an HP1α with basal band shift. The asterisk represents an HP1α species with metaphase-associated phosphorylation. The same membrane was immunoblotted with anti-α-tubulin and anti-TIF1β antibodies for loading controls. (C) Amino acid sequence of mouse HP1α. Light- and dark-shaded boxes indicate the CD and CSD, respectively. The sites of serine (S)-to-alanine (A) substitutions introduced in the following experiments are shown in boldface. (D) Phos-tag and standard WB analysis of wild-type and mutant HP1αs. WCLs were prepared from NIH 3T3 cells transiently expressing FLAG-tagged wild-type HP1α or HP1α with N-terminal (N-term: S11-14A) mutations, hinge (Hinge: S92, 93, 95, 97A) mutations, or both (N-term + hinge). Each FLAG-HP1α with an SAP-treated control was analyzed by phos-tag WB using an anti-FLAG antibody. The phosphorylation state of HP1α represented by each protein band was deduced from the distance of migration level of each band (Rf) and is indicated to the right of the phos-tag WB panel. (E) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the N-terminal region. The phosphorylation patterns of the indicated FLAG-HP1α species were analyzed by phos-tag WB using a higher concentration (80 μM) of phos-tag acrylamide. (F) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the hinge region. (G) The numbers of phosphorylated peptides identified by liquid chromatography (LC)-MS/MS analysis of affinity-purified HP1α. (H) Summary of the phosphorylation sites of mouse HP1α deduced from the experiments described above.
    Exosap It, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 15320 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exosap it/product/Thermo Fisher
    Average 99 stars, based on 15320 article reviews
    Price from $9.99 to $1999.99
    exosap it - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Qiagen hotstartaq polymerase
    Identification of phosphorylation sites of mouse HP1α. (A) The phosphorylation patterns of mouse HP1α analyzed by phos-tag Western blotting (phos-tag WB). Whole-cell lysates (WCLs) of NIH 3T3 cells were resolved on an SDS-PAGE gel containing phos-tag acrylamide (phos-tag) or on a standard SDS-PAGE gel (mock). As an unphosphorylated control, <t>SAP-treated</t> <t>WCL</t> was loaded on the same gel. HP1α was detected using an anti-HP1α antibody. (B) Phosphorylation patterns of mouse HP1α in cells arrested at different cell cycle stages. WCLs were prepared from NIH 3T3 cells grown under the indicated conditions and analyzed by phos-tag WB. The arrowhead indicates an HP1α with basal band shift. The asterisk represents an HP1α species with metaphase-associated phosphorylation. The same membrane was immunoblotted with anti-α-tubulin and anti-TIF1β antibodies for loading controls. (C) Amino acid sequence of mouse HP1α. Light- and dark-shaded boxes indicate the CD and CSD, respectively. The sites of serine (S)-to-alanine (A) substitutions introduced in the following experiments are shown in boldface. (D) Phos-tag and standard WB analysis of wild-type and mutant HP1αs. WCLs were prepared from NIH 3T3 cells transiently expressing FLAG-tagged wild-type HP1α or HP1α with N-terminal (N-term: S11-14A) mutations, hinge (Hinge: S92, 93, 95, 97A) mutations, or both (N-term + hinge). Each FLAG-HP1α with an SAP-treated control was analyzed by phos-tag WB using an anti-FLAG antibody. The phosphorylation state of HP1α represented by each protein band was deduced from the distance of migration level of each band (Rf) and is indicated to the right of the phos-tag WB panel. (E) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the N-terminal region. The phosphorylation patterns of the indicated FLAG-HP1α species were analyzed by phos-tag WB using a higher concentration (80 μM) of phos-tag acrylamide. (F) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the hinge region. (G) The numbers of phosphorylated peptides identified by liquid chromatography (LC)-MS/MS analysis of affinity-purified HP1α. (H) Summary of the phosphorylation sites of mouse HP1α deduced from the experiments described above.
    Hotstartaq Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2232 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstartaq polymerase/product/Qiagen
    Average 99 stars, based on 2232 article reviews
    Price from $9.99 to $1999.99
    hotstartaq polymerase - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    93
    Promega shrimp alkaline phosphatase
    Identification of phosphorylation sites of mouse HP1α. (A) The phosphorylation patterns of mouse HP1α analyzed by phos-tag Western blotting (phos-tag WB). Whole-cell lysates (WCLs) of NIH 3T3 cells were resolved on an SDS-PAGE gel containing phos-tag acrylamide (phos-tag) or on a standard SDS-PAGE gel (mock). As an unphosphorylated control, <t>SAP-treated</t> <t>WCL</t> was loaded on the same gel. HP1α was detected using an anti-HP1α antibody. (B) Phosphorylation patterns of mouse HP1α in cells arrested at different cell cycle stages. WCLs were prepared from NIH 3T3 cells grown under the indicated conditions and analyzed by phos-tag WB. The arrowhead indicates an HP1α with basal band shift. The asterisk represents an HP1α species with metaphase-associated phosphorylation. The same membrane was immunoblotted with anti-α-tubulin and anti-TIF1β antibodies for loading controls. (C) Amino acid sequence of mouse HP1α. Light- and dark-shaded boxes indicate the CD and CSD, respectively. The sites of serine (S)-to-alanine (A) substitutions introduced in the following experiments are shown in boldface. (D) Phos-tag and standard WB analysis of wild-type and mutant HP1αs. WCLs were prepared from NIH 3T3 cells transiently expressing FLAG-tagged wild-type HP1α or HP1α with N-terminal (N-term: S11-14A) mutations, hinge (Hinge: S92, 93, 95, 97A) mutations, or both (N-term + hinge). Each FLAG-HP1α with an SAP-treated control was analyzed by phos-tag WB using an anti-FLAG antibody. The phosphorylation state of HP1α represented by each protein band was deduced from the distance of migration level of each band (Rf) and is indicated to the right of the phos-tag WB panel. (E) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the N-terminal region. The phosphorylation patterns of the indicated FLAG-HP1α species were analyzed by phos-tag WB using a higher concentration (80 μM) of phos-tag acrylamide. (F) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the hinge region. (G) The numbers of phosphorylated peptides identified by liquid chromatography (LC)-MS/MS analysis of affinity-purified HP1α. (H) Summary of the phosphorylation sites of mouse HP1α deduced from the experiments described above.
    Shrimp Alkaline Phosphatase, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 950 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shrimp alkaline phosphatase/product/Promega
    Average 93 stars, based on 950 article reviews
    Price from $9.99 to $1999.99
    shrimp alkaline phosphatase - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    99
    Thermo Fisher shrimp alkaline phosphatase
    Identification of phosphorylation sites of mouse HP1α. (A) The phosphorylation patterns of mouse HP1α analyzed by phos-tag Western blotting (phos-tag WB). Whole-cell lysates (WCLs) of NIH 3T3 cells were resolved on an SDS-PAGE gel containing phos-tag acrylamide (phos-tag) or on a standard SDS-PAGE gel (mock). As an unphosphorylated control, <t>SAP-treated</t> <t>WCL</t> was loaded on the same gel. HP1α was detected using an anti-HP1α antibody. (B) Phosphorylation patterns of mouse HP1α in cells arrested at different cell cycle stages. WCLs were prepared from NIH 3T3 cells grown under the indicated conditions and analyzed by phos-tag WB. The arrowhead indicates an HP1α with basal band shift. The asterisk represents an HP1α species with metaphase-associated phosphorylation. The same membrane was immunoblotted with anti-α-tubulin and anti-TIF1β antibodies for loading controls. (C) Amino acid sequence of mouse HP1α. Light- and dark-shaded boxes indicate the CD and CSD, respectively. The sites of serine (S)-to-alanine (A) substitutions introduced in the following experiments are shown in boldface. (D) Phos-tag and standard WB analysis of wild-type and mutant HP1αs. WCLs were prepared from NIH 3T3 cells transiently expressing FLAG-tagged wild-type HP1α or HP1α with N-terminal (N-term: S11-14A) mutations, hinge (Hinge: S92, 93, 95, 97A) mutations, or both (N-term + hinge). Each FLAG-HP1α with an SAP-treated control was analyzed by phos-tag WB using an anti-FLAG antibody. The phosphorylation state of HP1α represented by each protein band was deduced from the distance of migration level of each band (Rf) and is indicated to the right of the phos-tag WB panel. (E) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the N-terminal region. The phosphorylation patterns of the indicated FLAG-HP1α species were analyzed by phos-tag WB using a higher concentration (80 μM) of phos-tag acrylamide. (F) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the hinge region. (G) The numbers of phosphorylated peptides identified by liquid chromatography (LC)-MS/MS analysis of affinity-purified HP1α. (H) Summary of the phosphorylation sites of mouse HP1α deduced from the experiments described above.
    Shrimp Alkaline Phosphatase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4320 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shrimp alkaline phosphatase/product/Thermo Fisher
    Average 99 stars, based on 4320 article reviews
    Price from $9.99 to $1999.99
    shrimp alkaline phosphatase - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher 3730xl dna analyzer
    Identification of phosphorylation sites of mouse HP1α. (A) The phosphorylation patterns of mouse HP1α analyzed by phos-tag Western blotting (phos-tag WB). Whole-cell lysates (WCLs) of NIH 3T3 cells were resolved on an SDS-PAGE gel containing phos-tag acrylamide (phos-tag) or on a standard SDS-PAGE gel (mock). As an unphosphorylated control, <t>SAP-treated</t> <t>WCL</t> was loaded on the same gel. HP1α was detected using an anti-HP1α antibody. (B) Phosphorylation patterns of mouse HP1α in cells arrested at different cell cycle stages. WCLs were prepared from NIH 3T3 cells grown under the indicated conditions and analyzed by phos-tag WB. The arrowhead indicates an HP1α with basal band shift. The asterisk represents an HP1α species with metaphase-associated phosphorylation. The same membrane was immunoblotted with anti-α-tubulin and anti-TIF1β antibodies for loading controls. (C) Amino acid sequence of mouse HP1α. Light- and dark-shaded boxes indicate the CD and CSD, respectively. The sites of serine (S)-to-alanine (A) substitutions introduced in the following experiments are shown in boldface. (D) Phos-tag and standard WB analysis of wild-type and mutant HP1αs. WCLs were prepared from NIH 3T3 cells transiently expressing FLAG-tagged wild-type HP1α or HP1α with N-terminal (N-term: S11-14A) mutations, hinge (Hinge: S92, 93, 95, 97A) mutations, or both (N-term + hinge). Each FLAG-HP1α with an SAP-treated control was analyzed by phos-tag WB using an anti-FLAG antibody. The phosphorylation state of HP1α represented by each protein band was deduced from the distance of migration level of each band (Rf) and is indicated to the right of the phos-tag WB panel. (E) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the N-terminal region. The phosphorylation patterns of the indicated FLAG-HP1α species were analyzed by phos-tag WB using a higher concentration (80 μM) of phos-tag acrylamide. (F) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the hinge region. (G) The numbers of phosphorylated peptides identified by liquid chromatography (LC)-MS/MS analysis of affinity-purified HP1α. (H) Summary of the phosphorylation sites of mouse HP1α deduced from the experiments described above.
    3730xl Dna Analyzer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 46651 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3730xl dna analyzer/product/Thermo Fisher
    Average 99 stars, based on 46651 article reviews
    Price from $9.99 to $1999.99
    3730xl dna analyzer - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    TaKaRa klenow fragment
    Identification of phosphorylation sites of mouse HP1α. (A) The phosphorylation patterns of mouse HP1α analyzed by phos-tag Western blotting (phos-tag WB). Whole-cell lysates (WCLs) of NIH 3T3 cells were resolved on an SDS-PAGE gel containing phos-tag acrylamide (phos-tag) or on a standard SDS-PAGE gel (mock). As an unphosphorylated control, <t>SAP-treated</t> <t>WCL</t> was loaded on the same gel. HP1α was detected using an anti-HP1α antibody. (B) Phosphorylation patterns of mouse HP1α in cells arrested at different cell cycle stages. WCLs were prepared from NIH 3T3 cells grown under the indicated conditions and analyzed by phos-tag WB. The arrowhead indicates an HP1α with basal band shift. The asterisk represents an HP1α species with metaphase-associated phosphorylation. The same membrane was immunoblotted with anti-α-tubulin and anti-TIF1β antibodies for loading controls. (C) Amino acid sequence of mouse HP1α. Light- and dark-shaded boxes indicate the CD and CSD, respectively. The sites of serine (S)-to-alanine (A) substitutions introduced in the following experiments are shown in boldface. (D) Phos-tag and standard WB analysis of wild-type and mutant HP1αs. WCLs were prepared from NIH 3T3 cells transiently expressing FLAG-tagged wild-type HP1α or HP1α with N-terminal (N-term: S11-14A) mutations, hinge (Hinge: S92, 93, 95, 97A) mutations, or both (N-term + hinge). Each FLAG-HP1α with an SAP-treated control was analyzed by phos-tag WB using an anti-FLAG antibody. The phosphorylation state of HP1α represented by each protein band was deduced from the distance of migration level of each band (Rf) and is indicated to the right of the phos-tag WB panel. (E) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the N-terminal region. The phosphorylation patterns of the indicated FLAG-HP1α species were analyzed by phos-tag WB using a higher concentration (80 μM) of phos-tag acrylamide. (F) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the hinge region. (G) The numbers of phosphorylated peptides identified by liquid chromatography (LC)-MS/MS analysis of affinity-purified HP1α. (H) Summary of the phosphorylation sites of mouse HP1α deduced from the experiments described above.
    Klenow Fragment, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 924 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/klenow fragment/product/TaKaRa
    Average 99 stars, based on 924 article reviews
    Price from $9.99 to $1999.99
    klenow fragment - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    TaKaRa pcr amplification
    Identification of phosphorylation sites of mouse HP1α. (A) The phosphorylation patterns of mouse HP1α analyzed by phos-tag Western blotting (phos-tag WB). Whole-cell lysates (WCLs) of NIH 3T3 cells were resolved on an SDS-PAGE gel containing phos-tag acrylamide (phos-tag) or on a standard SDS-PAGE gel (mock). As an unphosphorylated control, <t>SAP-treated</t> <t>WCL</t> was loaded on the same gel. HP1α was detected using an anti-HP1α antibody. (B) Phosphorylation patterns of mouse HP1α in cells arrested at different cell cycle stages. WCLs were prepared from NIH 3T3 cells grown under the indicated conditions and analyzed by phos-tag WB. The arrowhead indicates an HP1α with basal band shift. The asterisk represents an HP1α species with metaphase-associated phosphorylation. The same membrane was immunoblotted with anti-α-tubulin and anti-TIF1β antibodies for loading controls. (C) Amino acid sequence of mouse HP1α. Light- and dark-shaded boxes indicate the CD and CSD, respectively. The sites of serine (S)-to-alanine (A) substitutions introduced in the following experiments are shown in boldface. (D) Phos-tag and standard WB analysis of wild-type and mutant HP1αs. WCLs were prepared from NIH 3T3 cells transiently expressing FLAG-tagged wild-type HP1α or HP1α with N-terminal (N-term: S11-14A) mutations, hinge (Hinge: S92, 93, 95, 97A) mutations, or both (N-term + hinge). Each FLAG-HP1α with an SAP-treated control was analyzed by phos-tag WB using an anti-FLAG antibody. The phosphorylation state of HP1α represented by each protein band was deduced from the distance of migration level of each band (Rf) and is indicated to the right of the phos-tag WB panel. (E) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the N-terminal region. The phosphorylation patterns of the indicated FLAG-HP1α species were analyzed by phos-tag WB using a higher concentration (80 μM) of phos-tag acrylamide. (F) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the hinge region. (G) The numbers of phosphorylated peptides identified by liquid chromatography (LC)-MS/MS analysis of affinity-purified HP1α. (H) Summary of the phosphorylation sites of mouse HP1α deduced from the experiments described above.
    Pcr Amplification, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 21524 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr amplification/product/TaKaRa
    Average 99 stars, based on 21524 article reviews
    Price from $9.99 to $1999.99
    pcr amplification - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher pcr product purification kit
    Identification of phosphorylation sites of mouse HP1α. (A) The phosphorylation patterns of mouse HP1α analyzed by phos-tag Western blotting (phos-tag WB). Whole-cell lysates (WCLs) of NIH 3T3 cells were resolved on an SDS-PAGE gel containing phos-tag acrylamide (phos-tag) or on a standard SDS-PAGE gel (mock). As an unphosphorylated control, <t>SAP-treated</t> <t>WCL</t> was loaded on the same gel. HP1α was detected using an anti-HP1α antibody. (B) Phosphorylation patterns of mouse HP1α in cells arrested at different cell cycle stages. WCLs were prepared from NIH 3T3 cells grown under the indicated conditions and analyzed by phos-tag WB. The arrowhead indicates an HP1α with basal band shift. The asterisk represents an HP1α species with metaphase-associated phosphorylation. The same membrane was immunoblotted with anti-α-tubulin and anti-TIF1β antibodies for loading controls. (C) Amino acid sequence of mouse HP1α. Light- and dark-shaded boxes indicate the CD and CSD, respectively. The sites of serine (S)-to-alanine (A) substitutions introduced in the following experiments are shown in boldface. (D) Phos-tag and standard WB analysis of wild-type and mutant HP1αs. WCLs were prepared from NIH 3T3 cells transiently expressing FLAG-tagged wild-type HP1α or HP1α with N-terminal (N-term: S11-14A) mutations, hinge (Hinge: S92, 93, 95, 97A) mutations, or both (N-term + hinge). Each FLAG-HP1α with an SAP-treated control was analyzed by phos-tag WB using an anti-FLAG antibody. The phosphorylation state of HP1α represented by each protein band was deduced from the distance of migration level of each band (Rf) and is indicated to the right of the phos-tag WB panel. (E) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the N-terminal region. The phosphorylation patterns of the indicated FLAG-HP1α species were analyzed by phos-tag WB using a higher concentration (80 μM) of phos-tag acrylamide. (F) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the hinge region. (G) The numbers of phosphorylated peptides identified by liquid chromatography (LC)-MS/MS analysis of affinity-purified HP1α. (H) Summary of the phosphorylation sites of mouse HP1α deduced from the experiments described above.
    Pcr Product Purification Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr product purification kit/product/Thermo Fisher
    Average 99 stars, based on 135 article reviews
    Price from $9.99 to $1999.99
    pcr product purification kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    88
    Bio Basic Canada ez 10 spin column plasmid mini preps kit
    Identification of phosphorylation sites of mouse HP1α. (A) The phosphorylation patterns of mouse HP1α analyzed by phos-tag Western blotting (phos-tag WB). Whole-cell lysates (WCLs) of NIH 3T3 cells were resolved on an SDS-PAGE gel containing phos-tag acrylamide (phos-tag) or on a standard SDS-PAGE gel (mock). As an unphosphorylated control, <t>SAP-treated</t> <t>WCL</t> was loaded on the same gel. HP1α was detected using an anti-HP1α antibody. (B) Phosphorylation patterns of mouse HP1α in cells arrested at different cell cycle stages. WCLs were prepared from NIH 3T3 cells grown under the indicated conditions and analyzed by phos-tag WB. The arrowhead indicates an HP1α with basal band shift. The asterisk represents an HP1α species with metaphase-associated phosphorylation. The same membrane was immunoblotted with anti-α-tubulin and anti-TIF1β antibodies for loading controls. (C) Amino acid sequence of mouse HP1α. Light- and dark-shaded boxes indicate the CD and CSD, respectively. The sites of serine (S)-to-alanine (A) substitutions introduced in the following experiments are shown in boldface. (D) Phos-tag and standard WB analysis of wild-type and mutant HP1αs. WCLs were prepared from NIH 3T3 cells transiently expressing FLAG-tagged wild-type HP1α or HP1α with N-terminal (N-term: S11-14A) mutations, hinge (Hinge: S92, 93, 95, 97A) mutations, or both (N-term + hinge). Each FLAG-HP1α with an SAP-treated control was analyzed by phos-tag WB using an anti-FLAG antibody. The phosphorylation state of HP1α represented by each protein band was deduced from the distance of migration level of each band (Rf) and is indicated to the right of the phos-tag WB panel. (E) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the N-terminal region. The phosphorylation patterns of the indicated FLAG-HP1α species were analyzed by phos-tag WB using a higher concentration (80 μM) of phos-tag acrylamide. (F) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the hinge region. (G) The numbers of phosphorylated peptides identified by liquid chromatography (LC)-MS/MS analysis of affinity-purified HP1α. (H) Summary of the phosphorylation sites of mouse HP1α deduced from the experiments described above.
    Ez 10 Spin Column Plasmid Mini Preps Kit, supplied by Bio Basic Canada, used in various techniques. Bioz Stars score: 88/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ez 10 spin column plasmid mini preps kit/product/Bio Basic Canada
    Average 88 stars, based on 44 article reviews
    Price from $9.99 to $1999.99
    ez 10 spin column plasmid mini preps kit - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    99
    Qiagen qiaquick pcr purification kit
    Identification of phosphorylation sites of mouse HP1α. (A) The phosphorylation patterns of mouse HP1α analyzed by phos-tag Western blotting (phos-tag WB). Whole-cell lysates (WCLs) of NIH 3T3 cells were resolved on an SDS-PAGE gel containing phos-tag acrylamide (phos-tag) or on a standard SDS-PAGE gel (mock). As an unphosphorylated control, <t>SAP-treated</t> <t>WCL</t> was loaded on the same gel. HP1α was detected using an anti-HP1α antibody. (B) Phosphorylation patterns of mouse HP1α in cells arrested at different cell cycle stages. WCLs were prepared from NIH 3T3 cells grown under the indicated conditions and analyzed by phos-tag WB. The arrowhead indicates an HP1α with basal band shift. The asterisk represents an HP1α species with metaphase-associated phosphorylation. The same membrane was immunoblotted with anti-α-tubulin and anti-TIF1β antibodies for loading controls. (C) Amino acid sequence of mouse HP1α. Light- and dark-shaded boxes indicate the CD and CSD, respectively. The sites of serine (S)-to-alanine (A) substitutions introduced in the following experiments are shown in boldface. (D) Phos-tag and standard WB analysis of wild-type and mutant HP1αs. WCLs were prepared from NIH 3T3 cells transiently expressing FLAG-tagged wild-type HP1α or HP1α with N-terminal (N-term: S11-14A) mutations, hinge (Hinge: S92, 93, 95, 97A) mutations, or both (N-term + hinge). Each FLAG-HP1α with an SAP-treated control was analyzed by phos-tag WB using an anti-FLAG antibody. The phosphorylation state of HP1α represented by each protein band was deduced from the distance of migration level of each band (Rf) and is indicated to the right of the phos-tag WB panel. (E) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the N-terminal region. The phosphorylation patterns of the indicated FLAG-HP1α species were analyzed by phos-tag WB using a higher concentration (80 μM) of phos-tag acrylamide. (F) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the hinge region. (G) The numbers of phosphorylated peptides identified by liquid chromatography (LC)-MS/MS analysis of affinity-purified HP1α. (H) Summary of the phosphorylation sites of mouse HP1α deduced from the experiments described above.
    Qiaquick Pcr Purification Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 137129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaquick pcr purification kit/product/Qiagen
    Average 99 stars, based on 137129 article reviews
    Price from $9.99 to $1999.99
    qiaquick pcr purification kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    88
    Gene Codes Inc sequencher 4 1 2
    Identification of phosphorylation sites of mouse HP1α. (A) The phosphorylation patterns of mouse HP1α analyzed by phos-tag Western blotting (phos-tag WB). Whole-cell lysates (WCLs) of NIH 3T3 cells were resolved on an SDS-PAGE gel containing phos-tag acrylamide (phos-tag) or on a standard SDS-PAGE gel (mock). As an unphosphorylated control, <t>SAP-treated</t> <t>WCL</t> was loaded on the same gel. HP1α was detected using an anti-HP1α antibody. (B) Phosphorylation patterns of mouse HP1α in cells arrested at different cell cycle stages. WCLs were prepared from NIH 3T3 cells grown under the indicated conditions and analyzed by phos-tag WB. The arrowhead indicates an HP1α with basal band shift. The asterisk represents an HP1α species with metaphase-associated phosphorylation. The same membrane was immunoblotted with anti-α-tubulin and anti-TIF1β antibodies for loading controls. (C) Amino acid sequence of mouse HP1α. Light- and dark-shaded boxes indicate the CD and CSD, respectively. The sites of serine (S)-to-alanine (A) substitutions introduced in the following experiments are shown in boldface. (D) Phos-tag and standard WB analysis of wild-type and mutant HP1αs. WCLs were prepared from NIH 3T3 cells transiently expressing FLAG-tagged wild-type HP1α or HP1α with N-terminal (N-term: S11-14A) mutations, hinge (Hinge: S92, 93, 95, 97A) mutations, or both (N-term + hinge). Each FLAG-HP1α with an SAP-treated control was analyzed by phos-tag WB using an anti-FLAG antibody. The phosphorylation state of HP1α represented by each protein band was deduced from the distance of migration level of each band (Rf) and is indicated to the right of the phos-tag WB panel. (E) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the N-terminal region. The phosphorylation patterns of the indicated FLAG-HP1α species were analyzed by phos-tag WB using a higher concentration (80 μM) of phos-tag acrylamide. (F) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the hinge region. (G) The numbers of phosphorylated peptides identified by liquid chromatography (LC)-MS/MS analysis of affinity-purified HP1α. (H) Summary of the phosphorylation sites of mouse HP1α deduced from the experiments described above.
    Sequencher 4 1 2, supplied by Gene Codes Inc, used in various techniques. Bioz Stars score: 88/100, based on 143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequencher 4 1 2/product/Gene Codes Inc
    Average 88 stars, based on 143 article reviews
    Price from $9.99 to $1999.99
    sequencher 4 1 2 - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    94
    Thermo Fisher abi 3730 genetic analyzer
    Identification of phosphorylation sites of mouse HP1α. (A) The phosphorylation patterns of mouse HP1α analyzed by phos-tag Western blotting (phos-tag WB). Whole-cell lysates (WCLs) of NIH 3T3 cells were resolved on an SDS-PAGE gel containing phos-tag acrylamide (phos-tag) or on a standard SDS-PAGE gel (mock). As an unphosphorylated control, <t>SAP-treated</t> <t>WCL</t> was loaded on the same gel. HP1α was detected using an anti-HP1α antibody. (B) Phosphorylation patterns of mouse HP1α in cells arrested at different cell cycle stages. WCLs were prepared from NIH 3T3 cells grown under the indicated conditions and analyzed by phos-tag WB. The arrowhead indicates an HP1α with basal band shift. The asterisk represents an HP1α species with metaphase-associated phosphorylation. The same membrane was immunoblotted with anti-α-tubulin and anti-TIF1β antibodies for loading controls. (C) Amino acid sequence of mouse HP1α. Light- and dark-shaded boxes indicate the CD and CSD, respectively. The sites of serine (S)-to-alanine (A) substitutions introduced in the following experiments are shown in boldface. (D) Phos-tag and standard WB analysis of wild-type and mutant HP1αs. WCLs were prepared from NIH 3T3 cells transiently expressing FLAG-tagged wild-type HP1α or HP1α with N-terminal (N-term: S11-14A) mutations, hinge (Hinge: S92, 93, 95, 97A) mutations, or both (N-term + hinge). Each FLAG-HP1α with an SAP-treated control was analyzed by phos-tag WB using an anti-FLAG antibody. The phosphorylation state of HP1α represented by each protein band was deduced from the distance of migration level of each band (Rf) and is indicated to the right of the phos-tag WB panel. (E) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the N-terminal region. The phosphorylation patterns of the indicated FLAG-HP1α species were analyzed by phos-tag WB using a higher concentration (80 μM) of phos-tag acrylamide. (F) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the hinge region. (G) The numbers of phosphorylated peptides identified by liquid chromatography (LC)-MS/MS analysis of affinity-purified HP1α. (H) Summary of the phosphorylation sites of mouse HP1α deduced from the experiments described above.
    Abi 3730 Genetic Analyzer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2525 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi 3730 genetic analyzer/product/Thermo Fisher
    Average 94 stars, based on 2525 article reviews
    Price from $9.99 to $1999.99
    abi 3730 genetic analyzer - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Identification of phosphorylation sites of mouse HP1α. (A) The phosphorylation patterns of mouse HP1α analyzed by phos-tag Western blotting (phos-tag WB). Whole-cell lysates (WCLs) of NIH 3T3 cells were resolved on an SDS-PAGE gel containing phos-tag acrylamide (phos-tag) or on a standard SDS-PAGE gel (mock). As an unphosphorylated control, SAP-treated WCL was loaded on the same gel. HP1α was detected using an anti-HP1α antibody. (B) Phosphorylation patterns of mouse HP1α in cells arrested at different cell cycle stages. WCLs were prepared from NIH 3T3 cells grown under the indicated conditions and analyzed by phos-tag WB. The arrowhead indicates an HP1α with basal band shift. The asterisk represents an HP1α species with metaphase-associated phosphorylation. The same membrane was immunoblotted with anti-α-tubulin and anti-TIF1β antibodies for loading controls. (C) Amino acid sequence of mouse HP1α. Light- and dark-shaded boxes indicate the CD and CSD, respectively. The sites of serine (S)-to-alanine (A) substitutions introduced in the following experiments are shown in boldface. (D) Phos-tag and standard WB analysis of wild-type and mutant HP1αs. WCLs were prepared from NIH 3T3 cells transiently expressing FLAG-tagged wild-type HP1α or HP1α with N-terminal (N-term: S11-14A) mutations, hinge (Hinge: S92, 93, 95, 97A) mutations, or both (N-term + hinge). Each FLAG-HP1α with an SAP-treated control was analyzed by phos-tag WB using an anti-FLAG antibody. The phosphorylation state of HP1α represented by each protein band was deduced from the distance of migration level of each band (Rf) and is indicated to the right of the phos-tag WB panel. (E) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the N-terminal region. The phosphorylation patterns of the indicated FLAG-HP1α species were analyzed by phos-tag WB using a higher concentration (80 μM) of phos-tag acrylamide. (F) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the hinge region. (G) The numbers of phosphorylated peptides identified by liquid chromatography (LC)-MS/MS analysis of affinity-purified HP1α. (H) Summary of the phosphorylation sites of mouse HP1α deduced from the experiments described above.

    Journal: Molecular and Cellular Biology

    Article Title: N-Terminal Phosphorylation of HP1? Promotes Its Chromatin Binding ▿

    doi: 10.1128/MCB.01012-10

    Figure Lengend Snippet: Identification of phosphorylation sites of mouse HP1α. (A) The phosphorylation patterns of mouse HP1α analyzed by phos-tag Western blotting (phos-tag WB). Whole-cell lysates (WCLs) of NIH 3T3 cells were resolved on an SDS-PAGE gel containing phos-tag acrylamide (phos-tag) or on a standard SDS-PAGE gel (mock). As an unphosphorylated control, SAP-treated WCL was loaded on the same gel. HP1α was detected using an anti-HP1α antibody. (B) Phosphorylation patterns of mouse HP1α in cells arrested at different cell cycle stages. WCLs were prepared from NIH 3T3 cells grown under the indicated conditions and analyzed by phos-tag WB. The arrowhead indicates an HP1α with basal band shift. The asterisk represents an HP1α species with metaphase-associated phosphorylation. The same membrane was immunoblotted with anti-α-tubulin and anti-TIF1β antibodies for loading controls. (C) Amino acid sequence of mouse HP1α. Light- and dark-shaded boxes indicate the CD and CSD, respectively. The sites of serine (S)-to-alanine (A) substitutions introduced in the following experiments are shown in boldface. (D) Phos-tag and standard WB analysis of wild-type and mutant HP1αs. WCLs were prepared from NIH 3T3 cells transiently expressing FLAG-tagged wild-type HP1α or HP1α with N-terminal (N-term: S11-14A) mutations, hinge (Hinge: S92, 93, 95, 97A) mutations, or both (N-term + hinge). Each FLAG-HP1α with an SAP-treated control was analyzed by phos-tag WB using an anti-FLAG antibody. The phosphorylation state of HP1α represented by each protein band was deduced from the distance of migration level of each band (Rf) and is indicated to the right of the phos-tag WB panel. (E) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the N-terminal region. The phosphorylation patterns of the indicated FLAG-HP1α species were analyzed by phos-tag WB using a higher concentration (80 μM) of phos-tag acrylamide. (F) Phosphorylation state of FLAG-HP1α with each single amino acid substitution in the hinge region. (G) The numbers of phosphorylated peptides identified by liquid chromatography (LC)-MS/MS analysis of affinity-purified HP1α. (H) Summary of the phosphorylation sites of mouse HP1α deduced from the experiments described above.

    Article Snippet: For dephosphorylation, an aliquot of whole-cell lysate (WCL) was incubated with 0.08 U/ml shrimp alkaline phosphatase (SAP) (TaKaRa) for 3 to 6 h at 37°C.

    Techniques: Western Blot, SDS Page, Electrophoretic Mobility Shift Assay, Sequencing, Mutagenesis, Expressing, Migration, Concentration Assay, Liquid Chromatography, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Affinity Purification