shrimp alkaline phosphatase Roche Search Results


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  • 93
    Roche shrimp alkaline phosphatase
    IFN induction by NSV vRNAs depends on RIG-I and the 5′ triphosphate group. (A) Verification of knockdowns. Human 293T cells were treated with retroviral shRNA constructs directed against either RIG-I or MDA5, and cotransfected with expression constructs for HA-tagged MDA5 (left panels) or GFP-fused RIG-I (right panels). Western blot analysis using antibodies against the respective fusion tags is shown. Detection of cellular β-tubulin was used as an internal control. (B) Effect of shRNA knockdowns on IFN induction by viral <t>RNAs,</t> using the reporter constructs and RNA transfection protocols as described for Fig. 1A . The negative control shRNA construct (CTRL) targets the heat shock 70 interacting protein and was tested to have no effect on IFN induction (data not shown). (C) Genomic RNAs from ZEBOV and NiV were either mock treated, treated with <t>SAP,</t> or treated with SAP in the presence of the phosphatase inhibitor EDTA. IFN-β reporter assays and RNA transfections were performed as described for Fig. 1A . Mean values and standard deviations from 3 independent experiments are shown.
    Shrimp Alkaline Phosphatase, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 1353 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Roche rapid alkaline phosphatase
    IFN induction by NSV vRNAs depends on RIG-I and the 5′ triphosphate group. (A) Verification of knockdowns. Human 293T cells were treated with retroviral shRNA constructs directed against either RIG-I or MDA5, and cotransfected with expression constructs for HA-tagged MDA5 (left panels) or GFP-fused RIG-I (right panels). Western blot analysis using antibodies against the respective fusion tags is shown. Detection of cellular β-tubulin was used as an internal control. (B) Effect of shRNA knockdowns on IFN induction by viral <t>RNAs,</t> using the reporter constructs and RNA transfection protocols as described for Fig. 1A . The negative control shRNA construct (CTRL) targets the heat shock 70 interacting protein and was tested to have no effect on IFN induction (data not shown). (C) Genomic RNAs from ZEBOV and NiV were either mock treated, treated with <t>SAP,</t> or treated with SAP in the presence of the phosphatase inhibitor EDTA. IFN-β reporter assays and RNA transfections were performed as described for Fig. 1A . Mean values and standard deviations from 3 independent experiments are shown.
    Rapid Alkaline Phosphatase, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 675 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rapid alkaline phosphatase/product/Roche
    Average 90 stars, based on 675 article reviews
    Price from $9.99 to $1999.99
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    85
    Roche 45 u shrimp alkaline phosphatase
    IFN induction by NSV vRNAs depends on RIG-I and the 5′ triphosphate group. (A) Verification of knockdowns. Human 293T cells were treated with retroviral shRNA constructs directed against either RIG-I or MDA5, and cotransfected with expression constructs for HA-tagged MDA5 (left panels) or GFP-fused RIG-I (right panels). Western blot analysis using antibodies against the respective fusion tags is shown. Detection of cellular β-tubulin was used as an internal control. (B) Effect of shRNA knockdowns on IFN induction by viral <t>RNAs,</t> using the reporter constructs and RNA transfection protocols as described for Fig. 1A . The negative control shRNA construct (CTRL) targets the heat shock 70 interacting protein and was tested to have no effect on IFN induction (data not shown). (C) Genomic RNAs from ZEBOV and NiV were either mock treated, treated with <t>SAP,</t> or treated with SAP in the presence of the phosphatase inhibitor EDTA. IFN-β reporter assays and RNA transfections were performed as described for Fig. 1A . Mean values and standard deviations from 3 independent experiments are shown.
    45 U Shrimp Alkaline Phosphatase, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/45 u shrimp alkaline phosphatase/product/Roche
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    92
    Roche dephosphorylation
    IFN induction by NSV vRNAs depends on RIG-I and the 5′ triphosphate group. (A) Verification of knockdowns. Human 293T cells were treated with retroviral shRNA constructs directed against either RIG-I or MDA5, and cotransfected with expression constructs for HA-tagged MDA5 (left panels) or GFP-fused RIG-I (right panels). Western blot analysis using antibodies against the respective fusion tags is shown. Detection of cellular β-tubulin was used as an internal control. (B) Effect of shRNA knockdowns on IFN induction by viral <t>RNAs,</t> using the reporter constructs and RNA transfection protocols as described for Fig. 1A . The negative control shRNA construct (CTRL) targets the heat shock 70 interacting protein and was tested to have no effect on IFN induction (data not shown). (C) Genomic RNAs from ZEBOV and NiV were either mock treated, treated with <t>SAP,</t> or treated with SAP in the presence of the phosphatase inhibitor EDTA. IFN-β reporter assays and RNA transfections were performed as described for Fig. 1A . Mean values and standard deviations from 3 independent experiments are shown.
    Dephosphorylation, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Roche enzymatic treatment
    IFN induction by NSV vRNAs depends on RIG-I and the 5′ triphosphate group. (A) Verification of knockdowns. Human 293T cells were treated with retroviral shRNA constructs directed against either RIG-I or MDA5, and cotransfected with expression constructs for HA-tagged MDA5 (left panels) or GFP-fused RIG-I (right panels). Western blot analysis using antibodies against the respective fusion tags is shown. Detection of cellular β-tubulin was used as an internal control. (B) Effect of shRNA knockdowns on IFN induction by viral <t>RNAs,</t> using the reporter constructs and RNA transfection protocols as described for Fig. 1A . The negative control shRNA construct (CTRL) targets the heat shock 70 interacting protein and was tested to have no effect on IFN induction (data not shown). (C) Genomic RNAs from ZEBOV and NiV were either mock treated, treated with <t>SAP,</t> or treated with SAP in the presence of the phosphatase inhibitor EDTA. IFN-β reporter assays and RNA transfections were performed as described for Fig. 1A . Mean values and standard deviations from 3 independent experiments are shown.
    Enzymatic Treatment, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 272 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Roche sapexo treatment
    IFN induction by NSV vRNAs depends on RIG-I and the 5′ triphosphate group. (A) Verification of knockdowns. Human 293T cells were treated with retroviral shRNA constructs directed against either RIG-I or MDA5, and cotransfected with expression constructs for HA-tagged MDA5 (left panels) or GFP-fused RIG-I (right panels). Western blot analysis using antibodies against the respective fusion tags is shown. Detection of cellular β-tubulin was used as an internal control. (B) Effect of shRNA knockdowns on IFN induction by viral <t>RNAs,</t> using the reporter constructs and RNA transfection protocols as described for Fig. 1A . The negative control shRNA construct (CTRL) targets the heat shock 70 interacting protein and was tested to have no effect on IFN induction (data not shown). (C) Genomic RNAs from ZEBOV and NiV were either mock treated, treated with <t>SAP,</t> or treated with SAP in the presence of the phosphatase inhibitor EDTA. IFN-β reporter assays and RNA transfections were performed as described for Fig. 1A . Mean values and standard deviations from 3 independent experiments are shown.
    Sapexo Treatment, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    IFN induction by NSV vRNAs depends on RIG-I and the 5′ triphosphate group. (A) Verification of knockdowns. Human 293T cells were treated with retroviral shRNA constructs directed against either RIG-I or MDA5, and cotransfected with expression constructs for HA-tagged MDA5 (left panels) or GFP-fused RIG-I (right panels). Western blot analysis using antibodies against the respective fusion tags is shown. Detection of cellular β-tubulin was used as an internal control. (B) Effect of shRNA knockdowns on IFN induction by viral RNAs, using the reporter constructs and RNA transfection protocols as described for Fig. 1A . The negative control shRNA construct (CTRL) targets the heat shock 70 interacting protein and was tested to have no effect on IFN induction (data not shown). (C) Genomic RNAs from ZEBOV and NiV were either mock treated, treated with SAP, or treated with SAP in the presence of the phosphatase inhibitor EDTA. IFN-β reporter assays and RNA transfections were performed as described for Fig. 1A . Mean values and standard deviations from 3 independent experiments are shown.

    Journal: PLoS ONE

    Article Title: Processing of Genome 5? Termini as a Strategy of Negative-Strand RNA Viruses to Avoid RIG-I-Dependent Interferon Induction

    doi: 10.1371/journal.pone.0002032

    Figure Lengend Snippet: IFN induction by NSV vRNAs depends on RIG-I and the 5′ triphosphate group. (A) Verification of knockdowns. Human 293T cells were treated with retroviral shRNA constructs directed against either RIG-I or MDA5, and cotransfected with expression constructs for HA-tagged MDA5 (left panels) or GFP-fused RIG-I (right panels). Western blot analysis using antibodies against the respective fusion tags is shown. Detection of cellular β-tubulin was used as an internal control. (B) Effect of shRNA knockdowns on IFN induction by viral RNAs, using the reporter constructs and RNA transfection protocols as described for Fig. 1A . The negative control shRNA construct (CTRL) targets the heat shock 70 interacting protein and was tested to have no effect on IFN induction (data not shown). (C) Genomic RNAs from ZEBOV and NiV were either mock treated, treated with SAP, or treated with SAP in the presence of the phosphatase inhibitor EDTA. IFN-β reporter assays and RNA transfections were performed as described for Fig. 1A . Mean values and standard deviations from 3 independent experiments are shown.

    Article Snippet: Enzymatic treatments of RNA To remove 5′-terminal phosphates, purified RNAs were treated with 1 U of shrimp alkaline phosphatase (SAP) according to the protocol of the manufacturer (Roche).

    Techniques: shRNA, Construct, Expressing, Western Blot, Transfection, Negative Control

    Kinetics analysis of Tyr-48 phosphorylated and dephosphorylated cytochrome c with cytochrome c oxidase (CcO). Increasing amounts of isolated phosphorylated (open squares) and shrimp alkaline phosphatase-treated (closed squares) cytochrome c were added to solubilized CcO. CcO activity was measured with the polarographic method at 25 °C. CcO activity (turnover number, TN) is defined as consumed O 2 [μmoles]/(s · CcO [μmoles]).

    Journal: Biochimica et biophysica acta

    Article Title: Mammalian liver cytochrome c is tyrosine-48 phosphorylated in vivo, inhibiting mitochondrial respiration

    doi: 10.1016/j.bbabio.2008.04.023

    Figure Lengend Snippet: Kinetics analysis of Tyr-48 phosphorylated and dephosphorylated cytochrome c with cytochrome c oxidase (CcO). Increasing amounts of isolated phosphorylated (open squares) and shrimp alkaline phosphatase-treated (closed squares) cytochrome c were added to solubilized CcO. CcO activity was measured with the polarographic method at 25 °C. CcO activity (turnover number, TN) is defined as consumed O 2 [μmoles]/(s · CcO [μmoles]).

    Article Snippet: Unspecific phosphatase shrimp alkaline phosphatase (10 units, Roche, Indianapolis, IN) was employed to dephosphorylate Cyt c as previously described [ ] using 300 μL of a 40 μM Cyt c solution and overnight incubation at 30 °C.

    Techniques: Isolation, Activity Assay

    Gel and Western analysis of isolated cow liver Cyt c . A, Cyt c was purified under conditions preserving the in vivo phosphorylation status (lane 2) and treated with shrimp alkaline phosphatase (SAP, lane 3). Samples were applied to a 4–12% SDS-PAGE gradient gel and protein bands were detected using the silver staining method. Lane M, protein size marker (kDa); lane 1, Sigma Cyt c ; lane 4, EGF stimulated A431 cell lysate (positive control for Western analysis); lane 5, ovalbumin (negative control for Western analysis). B, for Western analysis, protein samples were applied to SDS-PAGE and subsequently transferred to a nitrocellulose membrane and analyzed using an anti-phosphotyrosine antibody (4G10, Upstate). Purified Cyt c (lane 2) produces a strong signal with the anti-phosphotyrosine antibody, whereas overnight treatment with SAP abolishes the signal (lane 3). Samples in lanes 1 – 5 as denoted in A. Protein sizes are indicated on the left.

    Journal: Biochimica et biophysica acta

    Article Title: Mammalian liver cytochrome c is tyrosine-48 phosphorylated in vivo, inhibiting mitochondrial respiration

    doi: 10.1016/j.bbabio.2008.04.023

    Figure Lengend Snippet: Gel and Western analysis of isolated cow liver Cyt c . A, Cyt c was purified under conditions preserving the in vivo phosphorylation status (lane 2) and treated with shrimp alkaline phosphatase (SAP, lane 3). Samples were applied to a 4–12% SDS-PAGE gradient gel and protein bands were detected using the silver staining method. Lane M, protein size marker (kDa); lane 1, Sigma Cyt c ; lane 4, EGF stimulated A431 cell lysate (positive control for Western analysis); lane 5, ovalbumin (negative control for Western analysis). B, for Western analysis, protein samples were applied to SDS-PAGE and subsequently transferred to a nitrocellulose membrane and analyzed using an anti-phosphotyrosine antibody (4G10, Upstate). Purified Cyt c (lane 2) produces a strong signal with the anti-phosphotyrosine antibody, whereas overnight treatment with SAP abolishes the signal (lane 3). Samples in lanes 1 – 5 as denoted in A. Protein sizes are indicated on the left.

    Article Snippet: Unspecific phosphatase shrimp alkaline phosphatase (10 units, Roche, Indianapolis, IN) was employed to dephosphorylate Cyt c as previously described [ ] using 300 μL of a 40 μM Cyt c solution and overnight incubation at 30 °C.

    Techniques: Western Blot, Isolation, Purification, Preserving, In Vivo, SDS Page, Silver Staining, Marker, Positive Control, Negative Control