sheep anti-digoxigenin fab Roche Search Results


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  • 79
    Roche sheep anti digoxigenin fab fragment
    Sheep Anti Digoxigenin Fab Fragment, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 79 stars, based on 9 article reviews
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    79
    Roche sheep anti digoxigenin anti dig fab fragments
    Inactivation of <t>antibody-POD</t> conjugate . Zebrafish embryos at 28 hpf were hybridized with dinitrophenyl-labeled shha and <t>digoxigenin-labeled</t> nkx6.1 RNA probes. (A, B) The expression patterns of shha (A) and nkx6.1 (B) as seen in single-color FISH experiments. In two-color experiments shha transcript was detected first using DyLight633-tyramide and nkx6.1 transcript was detected subsequently by FAM-tyramide. (C-H) Prior to the second round of detection, embryos were incubated for 10 minutes in PBS T (PBS plus 0.1% Tween-20) (C, D), PBS T containing 6% H 2 O 2 (E, F), or 100 mM glycine-HCl pH 2.0 (G, H). Single confocal sections of zebrafish brains are shown in the DyLight633-detection channel (Ch01) and in the FAM-detection channel (Ch02) from a lateral view and with anterior to the left. Images were recorded on a LSM510 microscope (Carl Zeiss) and false colored in ImageJ. Scale bar = 50 μm.
    Sheep Anti Digoxigenin Anti Dig Fab Fragments, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Roche sheep anti dig ap fab
    Inactivation of <t>antibody-POD</t> conjugate . Zebrafish embryos at 28 hpf were hybridized with dinitrophenyl-labeled shha and <t>digoxigenin-labeled</t> nkx6.1 RNA probes. (A, B) The expression patterns of shha (A) and nkx6.1 (B) as seen in single-color FISH experiments. In two-color experiments shha transcript was detected first using DyLight633-tyramide and nkx6.1 transcript was detected subsequently by FAM-tyramide. (C-H) Prior to the second round of detection, embryos were incubated for 10 minutes in PBS T (PBS plus 0.1% Tween-20) (C, D), PBS T containing 6% H 2 O 2 (E, F), or 100 mM glycine-HCl pH 2.0 (G, H). Single confocal sections of zebrafish brains are shown in the DyLight633-detection channel (Ch01) and in the FAM-detection channel (Ch02) from a lateral view and with anterior to the left. Images were recorded on a LSM510 microscope (Carl Zeiss) and false colored in ImageJ. Scale bar = 50 μm.
    Sheep Anti Dig Ap Fab, supplied by Roche, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Roche polyclonal sheep anti digoxigenin anti dig fab fragments
    Inactivation of <t>antibody-POD</t> conjugate . Zebrafish embryos at 28 hpf were hybridized with dinitrophenyl-labeled shha and <t>digoxigenin-labeled</t> nkx6.1 RNA probes. (A, B) The expression patterns of shha (A) and nkx6.1 (B) as seen in single-color FISH experiments. In two-color experiments shha transcript was detected first using DyLight633-tyramide and nkx6.1 transcript was detected subsequently by FAM-tyramide. (C-H) Prior to the second round of detection, embryos were incubated for 10 minutes in PBS T (PBS plus 0.1% Tween-20) (C, D), PBS T containing 6% H 2 O 2 (E, F), or 100 mM glycine-HCl pH 2.0 (G, H). Single confocal sections of zebrafish brains are shown in the DyLight633-detection channel (Ch01) and in the FAM-detection channel (Ch02) from a lateral view and with anterior to the left. Images were recorded on a LSM510 microscope (Carl Zeiss) and false colored in ImageJ. Scale bar = 50 μm.
    Polyclonal Sheep Anti Digoxigenin Anti Dig Fab Fragments, supplied by Roche, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Roche sheep anti digoxigenin fab fitc antibody
    Inactivation of <t>antibody-POD</t> conjugate . Zebrafish embryos at 28 hpf were hybridized with dinitrophenyl-labeled shha and <t>digoxigenin-labeled</t> nkx6.1 RNA probes. (A, B) The expression patterns of shha (A) and nkx6.1 (B) as seen in single-color FISH experiments. In two-color experiments shha transcript was detected first using DyLight633-tyramide and nkx6.1 transcript was detected subsequently by FAM-tyramide. (C-H) Prior to the second round of detection, embryos were incubated for 10 minutes in PBS T (PBS plus 0.1% Tween-20) (C, D), PBS T containing 6% H 2 O 2 (E, F), or 100 mM glycine-HCl pH 2.0 (G, H). Single confocal sections of zebrafish brains are shown in the DyLight633-detection channel (Ch01) and in the FAM-detection channel (Ch02) from a lateral view and with anterior to the left. Images were recorded on a LSM510 microscope (Carl Zeiss) and false colored in ImageJ. Scale bar = 50 μm.
    Sheep Anti Digoxigenin Fab Fitc Antibody, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Roche rhodamine conjugated sheep anti digoxigenin fab fragment
    Inactivation of <t>antibody-POD</t> conjugate . Zebrafish embryos at 28 hpf were hybridized with dinitrophenyl-labeled shha and <t>digoxigenin-labeled</t> nkx6.1 RNA probes. (A, B) The expression patterns of shha (A) and nkx6.1 (B) as seen in single-color FISH experiments. In two-color experiments shha transcript was detected first using DyLight633-tyramide and nkx6.1 transcript was detected subsequently by FAM-tyramide. (C-H) Prior to the second round of detection, embryos were incubated for 10 minutes in PBS T (PBS plus 0.1% Tween-20) (C, D), PBS T containing 6% H 2 O 2 (E, F), or 100 mM glycine-HCl pH 2.0 (G, H). Single confocal sections of zebrafish brains are shown in the DyLight633-detection channel (Ch01) and in the FAM-detection channel (Ch02) from a lateral view and with anterior to the left. Images were recorded on a LSM510 microscope (Carl Zeiss) and false colored in ImageJ. Scale bar = 50 μm.
    Rhodamine Conjugated Sheep Anti Digoxigenin Fab Fragment, supplied by Roche, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Roche alkaline phosphatase conjugated sheep anti digoxigenin fab fragment
    Inactivation of <t>antibody-POD</t> conjugate . Zebrafish embryos at 28 hpf were hybridized with dinitrophenyl-labeled shha and <t>digoxigenin-labeled</t> nkx6.1 RNA probes. (A, B) The expression patterns of shha (A) and nkx6.1 (B) as seen in single-color FISH experiments. In two-color experiments shha transcript was detected first using DyLight633-tyramide and nkx6.1 transcript was detected subsequently by FAM-tyramide. (C-H) Prior to the second round of detection, embryos were incubated for 10 minutes in PBS T (PBS plus 0.1% Tween-20) (C, D), PBS T containing 6% H 2 O 2 (E, F), or 100 mM glycine-HCl pH 2.0 (G, H). Single confocal sections of zebrafish brains are shown in the DyLight633-detection channel (Ch01) and in the FAM-detection channel (Ch02) from a lateral view and with anterior to the left. Images were recorded on a LSM510 microscope (Carl Zeiss) and false colored in ImageJ. Scale bar = 50 μm.
    Alkaline Phosphatase Conjugated Sheep Anti Digoxigenin Fab Fragment, supplied by Roche, used in various techniques. Bioz Stars score: 84/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Roche preabsorbed sheep anti dig ap fab
    Inactivation of <t>antibody-POD</t> conjugate . Zebrafish embryos at 28 hpf were hybridized with dinitrophenyl-labeled shha and <t>digoxigenin-labeled</t> nkx6.1 RNA probes. (A, B) The expression patterns of shha (A) and nkx6.1 (B) as seen in single-color FISH experiments. In two-color experiments shha transcript was detected first using DyLight633-tyramide and nkx6.1 transcript was detected subsequently by FAM-tyramide. (C-H) Prior to the second round of detection, embryos were incubated for 10 minutes in PBS T (PBS plus 0.1% Tween-20) (C, D), PBS T containing 6% H 2 O 2 (E, F), or 100 mM glycine-HCl pH 2.0 (G, H). Single confocal sections of zebrafish brains are shown in the DyLight633-detection channel (Ch01) and in the FAM-detection channel (Ch02) from a lateral view and with anterior to the left. Images were recorded on a LSM510 microscope (Carl Zeiss) and false colored in ImageJ. Scale bar = 50 μm.
    Preabsorbed Sheep Anti Dig Ap Fab, supplied by Roche, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Roche alkaline phosphatase conjugated sheep anti digoxigenin fab antibody
    Comparison of binding with <t>digoxigenin-conjugated</t> MAA in paraffin sections (A) and cryosections (B) of the porcine trachea . Only the cryosections showed clear positivity in the glands (black arrows) and the small blood vessels (blue arrows), while paraffin sections were completely negative.
    Alkaline Phosphatase Conjugated Sheep Anti Digoxigenin Fab Antibody, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    Roche peroxidase conjugated sheep anti digoxigenin antibody fab fragment
    Comparison of binding with <t>digoxigenin-conjugated</t> MAA in paraffin sections (A) and cryosections (B) of the porcine trachea . Only the cryosections showed clear positivity in the glands (black arrows) and the small blood vessels (blue arrows), while paraffin sections were completely negative.
    Peroxidase Conjugated Sheep Anti Digoxigenin Antibody Fab Fragment, supplied by Roche, used in various techniques. Bioz Stars score: 81/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    Roche fluorescein fitc conjugated sheep anti dig fab fragment
    Comparison of binding with <t>digoxigenin-conjugated</t> MAA in paraffin sections (A) and cryosections (B) of the porcine trachea . Only the cryosections showed clear positivity in the glands (black arrows) and the small blood vessels (blue arrows), while paraffin sections were completely negative.
    Fluorescein Fitc Conjugated Sheep Anti Dig Fab Fragment, supplied by Roche, used in various techniques. Bioz Stars score: 82/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Roche horseradish peroxidase hrp labeled sheep anti digoxigenin dig fab fragments hrp anti dig
    Different Gb 3 glycolipid contents in the proximal and distal colon. GalCer (3.3 nmol; lanes 1 and 6), Gb 3 (4.6 nmol; lanes 2 and 7), or an extract of the proximal colon (lanes 3 and 8), distal colon (lanes 4 and 9), or kidneys (lanes 5 and 10) was separated on a TLC plate. The amounts of extracts applied were equivalent to tissue dry weights of 0.83, 0.96, and 0.86 mg of the proximal colon, distal colon, and kidneys, respectively. Glycolipids were stained with orcinol-sulfuric acid (lanes 1 to 5) or probed with <t>DIG-Stx1B</t> plus <t>HRP-anti-DIG</t> antibodies (lanes 6 to 10). Glycolipids that bound to DIG-Stx1B with similar mobility to Gb 3 (arrows) were abundantly present in the distal colon, but were almost completely absent from the proximal colon. DIG-Stx1B stained purified Gb 3 (arrowheads), but not purified GalCer.
    Horseradish Peroxidase Hrp Labeled Sheep Anti Digoxigenin Dig Fab Fragments Hrp Anti Dig, supplied by Roche, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Roche sheep anti digoxygenin ap fab fragment
    Different Gb 3 glycolipid contents in the proximal and distal colon. GalCer (3.3 nmol; lanes 1 and 6), Gb 3 (4.6 nmol; lanes 2 and 7), or an extract of the proximal colon (lanes 3 and 8), distal colon (lanes 4 and 9), or kidneys (lanes 5 and 10) was separated on a TLC plate. The amounts of extracts applied were equivalent to tissue dry weights of 0.83, 0.96, and 0.86 mg of the proximal colon, distal colon, and kidneys, respectively. Glycolipids were stained with orcinol-sulfuric acid (lanes 1 to 5) or probed with <t>DIG-Stx1B</t> plus <t>HRP-anti-DIG</t> antibodies (lanes 6 to 10). Glycolipids that bound to DIG-Stx1B with similar mobility to Gb 3 (arrows) were abundantly present in the distal colon, but were almost completely absent from the proximal colon. DIG-Stx1B stained purified Gb 3 (arrowheads), but not purified GalCer.
    Sheep Anti Digoxygenin Ap Fab Fragment, supplied by Roche, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Roche ap conjugated sheep anti dig fab fragment
    Different Gb 3 glycolipid contents in the proximal and distal colon. GalCer (3.3 nmol; lanes 1 and 6), Gb 3 (4.6 nmol; lanes 2 and 7), or an extract of the proximal colon (lanes 3 and 8), distal colon (lanes 4 and 9), or kidneys (lanes 5 and 10) was separated on a TLC plate. The amounts of extracts applied were equivalent to tissue dry weights of 0.83, 0.96, and 0.86 mg of the proximal colon, distal colon, and kidneys, respectively. Glycolipids were stained with orcinol-sulfuric acid (lanes 1 to 5) or probed with <t>DIG-Stx1B</t> plus <t>HRP-anti-DIG</t> antibodies (lanes 6 to 10). Glycolipids that bound to DIG-Stx1B with similar mobility to Gb 3 (arrows) were abundantly present in the distal colon, but were almost completely absent from the proximal colon. DIG-Stx1B stained purified Gb 3 (arrowheads), but not purified GalCer.
    Ap Conjugated Sheep Anti Dig Fab Fragment, supplied by Roche, used in various techniques. Bioz Stars score: 80/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Roche sheep anti dig peroxidase fab fragments
    Different Gb 3 glycolipid contents in the proximal and distal colon. GalCer (3.3 nmol; lanes 1 and 6), Gb 3 (4.6 nmol; lanes 2 and 7), or an extract of the proximal colon (lanes 3 and 8), distal colon (lanes 4 and 9), or kidneys (lanes 5 and 10) was separated on a TLC plate. The amounts of extracts applied were equivalent to tissue dry weights of 0.83, 0.96, and 0.86 mg of the proximal colon, distal colon, and kidneys, respectively. Glycolipids were stained with orcinol-sulfuric acid (lanes 1 to 5) or probed with <t>DIG-Stx1B</t> plus <t>HRP-anti-DIG</t> antibodies (lanes 6 to 10). Glycolipids that bound to DIG-Stx1B with similar mobility to Gb 3 (arrows) were abundantly present in the distal colon, but were almost completely absent from the proximal colon. DIG-Stx1B stained purified Gb 3 (arrowheads), but not purified GalCer.
    Sheep Anti Dig Peroxidase Fab Fragments, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Roche alkaline phosphatase conjugated sheep anti dig antibody fab fragment
    Different Gb 3 glycolipid contents in the proximal and distal colon. GalCer (3.3 nmol; lanes 1 and 6), Gb 3 (4.6 nmol; lanes 2 and 7), or an extract of the proximal colon (lanes 3 and 8), distal colon (lanes 4 and 9), or kidneys (lanes 5 and 10) was separated on a TLC plate. The amounts of extracts applied were equivalent to tissue dry weights of 0.83, 0.96, and 0.86 mg of the proximal colon, distal colon, and kidneys, respectively. Glycolipids were stained with orcinol-sulfuric acid (lanes 1 to 5) or probed with <t>DIG-Stx1B</t> plus <t>HRP-anti-DIG</t> antibodies (lanes 6 to 10). Glycolipids that bound to DIG-Stx1B with similar mobility to Gb 3 (arrows) were abundantly present in the distal colon, but were almost completely absent from the proximal colon. DIG-Stx1B stained purified Gb 3 (arrowheads), but not purified GalCer.
    Alkaline Phosphatase Conjugated Sheep Anti Dig Antibody Fab Fragment, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 79 stars, based on 8 article reviews
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    78
    Roche fluorescein isothiocyanate fitc conjugated sheep anti dig fab fragment
    Different Gb 3 glycolipid contents in the proximal and distal colon. GalCer (3.3 nmol; lanes 1 and 6), Gb 3 (4.6 nmol; lanes 2 and 7), or an extract of the proximal colon (lanes 3 and 8), distal colon (lanes 4 and 9), or kidneys (lanes 5 and 10) was separated on a TLC plate. The amounts of extracts applied were equivalent to tissue dry weights of 0.83, 0.96, and 0.86 mg of the proximal colon, distal colon, and kidneys, respectively. Glycolipids were stained with orcinol-sulfuric acid (lanes 1 to 5) or probed with <t>DIG-Stx1B</t> plus <t>HRP-anti-DIG</t> antibodies (lanes 6 to 10). Glycolipids that bound to DIG-Stx1B with similar mobility to Gb 3 (arrows) were abundantly present in the distal colon, but were almost completely absent from the proximal colon. DIG-Stx1B stained purified Gb 3 (arrowheads), but not purified GalCer.
    Fluorescein Isothiocyanate Fitc Conjugated Sheep Anti Dig Fab Fragment, supplied by Roche, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Roche non fluorescent detection sheep anti dig ap fab
    Different Gb 3 glycolipid contents in the proximal and distal colon. GalCer (3.3 nmol; lanes 1 and 6), Gb 3 (4.6 nmol; lanes 2 and 7), or an extract of the proximal colon (lanes 3 and 8), distal colon (lanes 4 and 9), or kidneys (lanes 5 and 10) was separated on a TLC plate. The amounts of extracts applied were equivalent to tissue dry weights of 0.83, 0.96, and 0.86 mg of the proximal colon, distal colon, and kidneys, respectively. Glycolipids were stained with orcinol-sulfuric acid (lanes 1 to 5) or probed with <t>DIG-Stx1B</t> plus <t>HRP-anti-DIG</t> antibodies (lanes 6 to 10). Glycolipids that bound to DIG-Stx1B with similar mobility to Gb 3 (arrows) were abundantly present in the distal colon, but were almost completely absent from the proximal colon. DIG-Stx1B stained purified Gb 3 (arrowheads), but not purified GalCer.
    Non Fluorescent Detection Sheep Anti Dig Ap Fab, supplied by Roche, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Roche alkaline phophatase conjugated sheep anti digoxigenin fab fragments
    Different Gb 3 glycolipid contents in the proximal and distal colon. GalCer (3.3 nmol; lanes 1 and 6), Gb 3 (4.6 nmol; lanes 2 and 7), or an extract of the proximal colon (lanes 3 and 8), distal colon (lanes 4 and 9), or kidneys (lanes 5 and 10) was separated on a TLC plate. The amounts of extracts applied were equivalent to tissue dry weights of 0.83, 0.96, and 0.86 mg of the proximal colon, distal colon, and kidneys, respectively. Glycolipids were stained with orcinol-sulfuric acid (lanes 1 to 5) or probed with <t>DIG-Stx1B</t> plus <t>HRP-anti-DIG</t> antibodies (lanes 6 to 10). Glycolipids that bound to DIG-Stx1B with similar mobility to Gb 3 (arrows) were abundantly present in the distal colon, but were almost completely absent from the proximal colon. DIG-Stx1B stained purified Gb 3 (arrowheads), but not purified GalCer.
    Alkaline Phophatase Conjugated Sheep Anti Digoxigenin Fab Fragments, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Roche hrp conjugated sheep anti dig fab fragments
    Different Gb 3 glycolipid contents in the proximal and distal colon. GalCer (3.3 nmol; lanes 1 and 6), Gb 3 (4.6 nmol; lanes 2 and 7), or an extract of the proximal colon (lanes 3 and 8), distal colon (lanes 4 and 9), or kidneys (lanes 5 and 10) was separated on a TLC plate. The amounts of extracts applied were equivalent to tissue dry weights of 0.83, 0.96, and 0.86 mg of the proximal colon, distal colon, and kidneys, respectively. Glycolipids were stained with orcinol-sulfuric acid (lanes 1 to 5) or probed with <t>DIG-Stx1B</t> plus <t>HRP-anti-DIG</t> antibodies (lanes 6 to 10). Glycolipids that bound to DIG-Stx1B with similar mobility to Gb 3 (arrows) were abundantly present in the distal colon, but were almost completely absent from the proximal colon. DIG-Stx1B stained purified Gb 3 (arrowheads), but not purified GalCer.
    Hrp Conjugated Sheep Anti Dig Fab Fragments, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Roche peroxidase conjugated sheep anti digoxigenin fab fragments
    Different Gb 3 glycolipid contents in the proximal and distal colon. GalCer (3.3 nmol; lanes 1 and 6), Gb 3 (4.6 nmol; lanes 2 and 7), or an extract of the proximal colon (lanes 3 and 8), distal colon (lanes 4 and 9), or kidneys (lanes 5 and 10) was separated on a TLC plate. The amounts of extracts applied were equivalent to tissue dry weights of 0.83, 0.96, and 0.86 mg of the proximal colon, distal colon, and kidneys, respectively. Glycolipids were stained with orcinol-sulfuric acid (lanes 1 to 5) or probed with <t>DIG-Stx1B</t> plus <t>HRP-anti-DIG</t> antibodies (lanes 6 to 10). Glycolipids that bound to DIG-Stx1B with similar mobility to Gb 3 (arrows) were abundantly present in the distal colon, but were almost completely absent from the proximal colon. DIG-Stx1B stained purified Gb 3 (arrowheads), but not purified GalCer.
    Peroxidase Conjugated Sheep Anti Digoxigenin Fab Fragments, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Roche sheep anti digoxigenin ap conjugated fabs
    Different Gb 3 glycolipid contents in the proximal and distal colon. GalCer (3.3 nmol; lanes 1 and 6), Gb 3 (4.6 nmol; lanes 2 and 7), or an extract of the proximal colon (lanes 3 and 8), distal colon (lanes 4 and 9), or kidneys (lanes 5 and 10) was separated on a TLC plate. The amounts of extracts applied were equivalent to tissue dry weights of 0.83, 0.96, and 0.86 mg of the proximal colon, distal colon, and kidneys, respectively. Glycolipids were stained with orcinol-sulfuric acid (lanes 1 to 5) or probed with <t>DIG-Stx1B</t> plus <t>HRP-anti-DIG</t> antibodies (lanes 6 to 10). Glycolipids that bound to DIG-Stx1B with similar mobility to Gb 3 (arrows) were abundantly present in the distal colon, but were almost completely absent from the proximal colon. DIG-Stx1B stained purified Gb 3 (arrowheads), but not purified GalCer.
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    Image Search Results


    Inactivation of antibody-POD conjugate . Zebrafish embryos at 28 hpf were hybridized with dinitrophenyl-labeled shha and digoxigenin-labeled nkx6.1 RNA probes. (A, B) The expression patterns of shha (A) and nkx6.1 (B) as seen in single-color FISH experiments. In two-color experiments shha transcript was detected first using DyLight633-tyramide and nkx6.1 transcript was detected subsequently by FAM-tyramide. (C-H) Prior to the second round of detection, embryos were incubated for 10 minutes in PBS T (PBS plus 0.1% Tween-20) (C, D), PBS T containing 6% H 2 O 2 (E, F), or 100 mM glycine-HCl pH 2.0 (G, H). Single confocal sections of zebrafish brains are shown in the DyLight633-detection channel (Ch01) and in the FAM-detection channel (Ch02) from a lateral view and with anterior to the left. Images were recorded on a LSM510 microscope (Carl Zeiss) and false colored in ImageJ. Scale bar = 50 μm.

    Journal: Neural Development

    Article Title: Multicolor fluorescent in situ hybridization to define abutting and overlapping gene expression in the embryonic zebrafish brain

    doi: 10.1186/1749-8104-6-10

    Figure Lengend Snippet: Inactivation of antibody-POD conjugate . Zebrafish embryos at 28 hpf were hybridized with dinitrophenyl-labeled shha and digoxigenin-labeled nkx6.1 RNA probes. (A, B) The expression patterns of shha (A) and nkx6.1 (B) as seen in single-color FISH experiments. In two-color experiments shha transcript was detected first using DyLight633-tyramide and nkx6.1 transcript was detected subsequently by FAM-tyramide. (C-H) Prior to the second round of detection, embryos were incubated for 10 minutes in PBS T (PBS plus 0.1% Tween-20) (C, D), PBS T containing 6% H 2 O 2 (E, F), or 100 mM glycine-HCl pH 2.0 (G, H). Single confocal sections of zebrafish brains are shown in the DyLight633-detection channel (Ch01) and in the FAM-detection channel (Ch02) from a lateral view and with anterior to the left. Images were recorded on a LSM510 microscope (Carl Zeiss) and false colored in ImageJ. Scale bar = 50 μm.

    Article Snippet: Sheep-anti-digoxigenin-POD Fab fragments (Roche 11207733910) were diluted 1:500, anti-dinitrophenyl-POD (PerkinElmer TSA Plus DNP System NEL747A001KT) was diluted 1:100 and the rabbit-anti-fluorescein/Oregon Green 488-POD (Molecular Probes: Eugene, OR, USA A21253) was diluted 1:500.

    Techniques: Labeling, Expressing, Fluorescence In Situ Hybridization, Incubation, Microscopy

    Positive effects of substituted phenol compounds on the TSA-POD reaction . Lateral views of 1-dpf zebrafish embryos hybridized with a digoxigenin-labeled antisense RNA probe specific for nkx6.1 are shown with anterior to the left. (A) Control (CTL); (B, C) vanillin added; (D, E) 4-iodophenol added. In all embryos shown, dextran sulfate was included in the hybridization and TSA-POD reaction. Above each panel signal-to-noise ratios (s/n) and compound concentrations are indicated. Black and white pictures were recorded with identical exposure times. Images were false-colored with help of the ImageJ software and no further adjustments or other image processing were performed. Scale bar = 100 μm.

    Journal: Neural Development

    Article Title: Multicolor fluorescent in situ hybridization to define abutting and overlapping gene expression in the embryonic zebrafish brain

    doi: 10.1186/1749-8104-6-10

    Figure Lengend Snippet: Positive effects of substituted phenol compounds on the TSA-POD reaction . Lateral views of 1-dpf zebrafish embryos hybridized with a digoxigenin-labeled antisense RNA probe specific for nkx6.1 are shown with anterior to the left. (A) Control (CTL); (B, C) vanillin added; (D, E) 4-iodophenol added. In all embryos shown, dextran sulfate was included in the hybridization and TSA-POD reaction. Above each panel signal-to-noise ratios (s/n) and compound concentrations are indicated. Black and white pictures were recorded with identical exposure times. Images were false-colored with help of the ImageJ software and no further adjustments or other image processing were performed. Scale bar = 100 μm.

    Article Snippet: Sheep-anti-digoxigenin-POD Fab fragments (Roche 11207733910) were diluted 1:500, anti-dinitrophenyl-POD (PerkinElmer TSA Plus DNP System NEL747A001KT) was diluted 1:100 and the rabbit-anti-fluorescein/Oregon Green 488-POD (Molecular Probes: Eugene, OR, USA A21253) was diluted 1:500.

    Techniques: Labeling, CTL Assay, Hybridization, Software

    Bench-made fluorescent tyramides applied in zebrafish whole-mount FISH . Embryos were viewed on an Axioplan II microscope (Carl Zeiss). For excitation, a mercury burner (HBO 103 OSRAM) was used. Lateral views of zebrafish brains are shown with anterior to the left. Scale bar = 100 μm. ( A-C ) Zebrafish embryos at 28 hpf hybridized to a digoxigenin-labeled nkx6.1 antisense RNA probe. Images were captured with an Orca digital camera (Hamamatsu). As POD substrates, three different bench-made fluorogenic tyramides at a 1:250 dilution were used: (A) FAM-tyramide (exposure time, 100 ms; Chroma-filter 41001); (B) TAMRA-tyramide (exposure time, 4 ms; Chroma-filter 41002b); and (C) DyLight633-tyramide (exposure time, 120 ms; Chroma-filter 41008). ( D-F ) Zebrafish embryos at 24 hpf hybridized to a digoxigenin-labeled shha antisense RNA probe. Images were recorded with an Axiocam digital color camera (Carl Zeiss) using identical exposure times. Transcript distribution was visualized with (D) fluorescein-tyramide from Perkin Elmer (SAT701B001EA) at a 1:100 dilution, (E) bench-made FAM-tyramide at a 1:250 dilution, or (F) a 1:100 dilution.

    Journal: Neural Development

    Article Title: Multicolor fluorescent in situ hybridization to define abutting and overlapping gene expression in the embryonic zebrafish brain

    doi: 10.1186/1749-8104-6-10

    Figure Lengend Snippet: Bench-made fluorescent tyramides applied in zebrafish whole-mount FISH . Embryos were viewed on an Axioplan II microscope (Carl Zeiss). For excitation, a mercury burner (HBO 103 OSRAM) was used. Lateral views of zebrafish brains are shown with anterior to the left. Scale bar = 100 μm. ( A-C ) Zebrafish embryos at 28 hpf hybridized to a digoxigenin-labeled nkx6.1 antisense RNA probe. Images were captured with an Orca digital camera (Hamamatsu). As POD substrates, three different bench-made fluorogenic tyramides at a 1:250 dilution were used: (A) FAM-tyramide (exposure time, 100 ms; Chroma-filter 41001); (B) TAMRA-tyramide (exposure time, 4 ms; Chroma-filter 41002b); and (C) DyLight633-tyramide (exposure time, 120 ms; Chroma-filter 41008). ( D-F ) Zebrafish embryos at 24 hpf hybridized to a digoxigenin-labeled shha antisense RNA probe. Images were recorded with an Axiocam digital color camera (Carl Zeiss) using identical exposure times. Transcript distribution was visualized with (D) fluorescein-tyramide from Perkin Elmer (SAT701B001EA) at a 1:100 dilution, (E) bench-made FAM-tyramide at a 1:250 dilution, or (F) a 1:100 dilution.

    Article Snippet: Sheep-anti-digoxigenin-POD Fab fragments (Roche 11207733910) were diluted 1:500, anti-dinitrophenyl-POD (PerkinElmer TSA Plus DNP System NEL747A001KT) was diluted 1:100 and the rabbit-anti-fluorescein/Oregon Green 488-POD (Molecular Probes: Eugene, OR, USA A21253) was diluted 1:500.

    Techniques: Fluorescence In Situ Hybridization, Microscopy, Labeling, Mass Spectrometry

    Optimization of hybridization and POD reaction by dextran sulfate . (A, C, E, G) Lateral views of 1-dpf zebrafish embryos hybridized with a digoxigenin-labeled antisense RNA probe specific for dbx1a are shown with anterior to the left. Above each panel signal-to-noise ratios (s/n) are indicated and whether dextran sulfate was added (+) or not (-) to the hybridization buffer (Hyb) and/or TSA reaction. (B, D, F, H) Embryos hybridized without probe were used as negative controls to assess background noise. Black-and-white pictures were recorded with identical exposure times using an Orca digital camera (Hamamatsu) on an Axioplan II microscope (Carl Zeiss). Images were false-colored with help of the ImageJ software and no further adjustments or other image processing was performed. Scale bar = 100 μm.

    Journal: Neural Development

    Article Title: Multicolor fluorescent in situ hybridization to define abutting and overlapping gene expression in the embryonic zebrafish brain

    doi: 10.1186/1749-8104-6-10

    Figure Lengend Snippet: Optimization of hybridization and POD reaction by dextran sulfate . (A, C, E, G) Lateral views of 1-dpf zebrafish embryos hybridized with a digoxigenin-labeled antisense RNA probe specific for dbx1a are shown with anterior to the left. Above each panel signal-to-noise ratios (s/n) are indicated and whether dextran sulfate was added (+) or not (-) to the hybridization buffer (Hyb) and/or TSA reaction. (B, D, F, H) Embryos hybridized without probe were used as negative controls to assess background noise. Black-and-white pictures were recorded with identical exposure times using an Orca digital camera (Hamamatsu) on an Axioplan II microscope (Carl Zeiss). Images were false-colored with help of the ImageJ software and no further adjustments or other image processing was performed. Scale bar = 100 μm.

    Article Snippet: Sheep-anti-digoxigenin-POD Fab fragments (Roche 11207733910) were diluted 1:500, anti-dinitrophenyl-POD (PerkinElmer TSA Plus DNP System NEL747A001KT) was diluted 1:100 and the rabbit-anti-fluorescein/Oregon Green 488-POD (Molecular Probes: Eugene, OR, USA A21253) was diluted 1:500.

    Techniques: Hybridization, Labeling, Microscopy, Software

    A) Effects of acute ethanol on MCH cell density as measured by digoxigenin-labeled ISH. The data (mean ± SEM) showed a significant increase in expression of MCH in the LH and a reduction in expression of MCH in the ZI at the higher dose of ethanol (2.5 g/kg) as compared with the water group (*p

    Journal: Physiology & behavior

    Article Title: Role of melanin-concentrating hormone in the control of ethanol consumption: Region-specific effects revealed by expression and injection studies

    doi: 10.1016/j.physbeh.2010.07.009

    Figure Lengend Snippet: A) Effects of acute ethanol on MCH cell density as measured by digoxigenin-labeled ISH. The data (mean ± SEM) showed a significant increase in expression of MCH in the LH and a reduction in expression of MCH in the ZI at the higher dose of ethanol (2.5 g/kg) as compared with the water group (*p

    Article Snippet: AP-conjugated sheep anti-digoxigenin Fab fragments (1:1000, Roche, Nutley, NJ) and NBT / BCIP (Roche, Nutley, NJ) were used to visualize the signal.

    Techniques: Labeling, In Situ Hybridization, Expressing

    Comparison of binding with digoxigenin-conjugated MAA in paraffin sections (A) and cryosections (B) of the porcine trachea . Only the cryosections showed clear positivity in the glands (black arrows) and the small blood vessels (blue arrows), while paraffin sections were completely negative.

    Journal: Virology Journal

    Article Title: Replication of avian, human and swine influenza viruses in porcine respiratory explants and association with sialic acid distribution

    doi: 10.1186/1743-422X-7-38

    Figure Lengend Snippet: Comparison of binding with digoxigenin-conjugated MAA in paraffin sections (A) and cryosections (B) of the porcine trachea . Only the cryosections showed clear positivity in the glands (black arrows) and the small blood vessels (blue arrows), while paraffin sections were completely negative.

    Article Snippet: Subsequently, the slides were incubated with 1:200 sheep alkaline phosphatase conjugated anti-digoxigenin Fab fragments (Roche) and developed with New Fuchsin (Dako).

    Techniques: Binding Assay

    Influence of the conjugation method of MAL-I and -II lectins on the staining intensities in duck small intestines . Biotinylated MAL-I (A) and MAL-II (a) both resulted in epithelial cell binding (black arrows), but MAL-II (a) was additionally staining the goblet cells (red arrow). For both lectins binding was abolished by sialidase treatment of the sections (B, b). Digoxigenin labelled MAL-I (C) and MAL-II (c) failed to bind to the same tissues.

    Journal: Virology Journal

    Article Title: Replication of avian, human and swine influenza viruses in porcine respiratory explants and association with sialic acid distribution

    doi: 10.1186/1743-422X-7-38

    Figure Lengend Snippet: Influence of the conjugation method of MAL-I and -II lectins on the staining intensities in duck small intestines . Biotinylated MAL-I (A) and MAL-II (a) both resulted in epithelial cell binding (black arrows), but MAL-II (a) was additionally staining the goblet cells (red arrow). For both lectins binding was abolished by sialidase treatment of the sections (B, b). Digoxigenin labelled MAL-I (C) and MAL-II (c) failed to bind to the same tissues.

    Article Snippet: Subsequently, the slides were incubated with 1:200 sheep alkaline phosphatase conjugated anti-digoxigenin Fab fragments (Roche) and developed with New Fuchsin (Dako).

    Techniques: Conjugation Assay, Staining, Binding Assay

    Different Gb 3 glycolipid contents in the proximal and distal colon. GalCer (3.3 nmol; lanes 1 and 6), Gb 3 (4.6 nmol; lanes 2 and 7), or an extract of the proximal colon (lanes 3 and 8), distal colon (lanes 4 and 9), or kidneys (lanes 5 and 10) was separated on a TLC plate. The amounts of extracts applied were equivalent to tissue dry weights of 0.83, 0.96, and 0.86 mg of the proximal colon, distal colon, and kidneys, respectively. Glycolipids were stained with orcinol-sulfuric acid (lanes 1 to 5) or probed with DIG-Stx1B plus HRP-anti-DIG antibodies (lanes 6 to 10). Glycolipids that bound to DIG-Stx1B with similar mobility to Gb 3 (arrows) were abundantly present in the distal colon, but were almost completely absent from the proximal colon. DIG-Stx1B stained purified Gb 3 (arrowheads), but not purified GalCer.

    Journal: Infection and Immunity

    Article Title: Restricted Expression of Shiga Toxin Binding Sites on Mucosal Epithelium of Mouse Distal Colon

    doi: 10.1128/IAI.71.2.985-990.2003

    Figure Lengend Snippet: Different Gb 3 glycolipid contents in the proximal and distal colon. GalCer (3.3 nmol; lanes 1 and 6), Gb 3 (4.6 nmol; lanes 2 and 7), or an extract of the proximal colon (lanes 3 and 8), distal colon (lanes 4 and 9), or kidneys (lanes 5 and 10) was separated on a TLC plate. The amounts of extracts applied were equivalent to tissue dry weights of 0.83, 0.96, and 0.86 mg of the proximal colon, distal colon, and kidneys, respectively. Glycolipids were stained with orcinol-sulfuric acid (lanes 1 to 5) or probed with DIG-Stx1B plus HRP-anti-DIG antibodies (lanes 6 to 10). Glycolipids that bound to DIG-Stx1B with similar mobility to Gb 3 (arrows) were abundantly present in the distal colon, but were almost completely absent from the proximal colon. DIG-Stx1B stained purified Gb 3 (arrowheads), but not purified GalCer.

    Article Snippet: Horseradish peroxidase (HRP)-labeled sheep anti-digoxigenin (DIG) Fab fragments (HRP-anti-DIG) were purchased from Roche Diagnostics (Tokyo, Japan).

    Techniques: Thin Layer Chromatography, Staining, Purification

    miR-506 expression is elevated in cholangiocytes from patients with PBC. A, in situ hybridization of miR-506 expression using LNA-modified digoxigenin-labeled miR-506 specific or scrambled probes (as controls) was examined using formalin-fixed paraffin-embedded

    Journal: The Journal of Biological Chemistry

    Article Title: Post-translational Regulation of the Type III Inositol 1,4,5-Trisphosphate Receptor by miRNA-506 *

    doi: 10.1074/jbc.M114.587030

    Figure Lengend Snippet: miR-506 expression is elevated in cholangiocytes from patients with PBC. A, in situ hybridization of miR-506 expression using LNA-modified digoxigenin-labeled miR-506 specific or scrambled probes (as controls) was examined using formalin-fixed paraffin-embedded

    Article Snippet: Afterward, they were incubated overnight at 4 °C with a 1:50 dilution of sheep anti-digoxigenin peroxidase conjugate (anti-digoxigenin POD Fab fragments, Roche Applied Science) and 1:300 dilution of pan-cytokeratin antibodies (polyclonal rabbit anti-cow Cytokeratin Wide Spectrum Screening, WSS, Dako Labs) in goat serum/BSA/PBS to label bile ducts.

    Techniques: Expressing, In Situ Hybridization, Modification, Labeling, Formalin-fixed Paraffin-Embedded