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    • sh800z cell sorter  (Sony)
    86
    Sony sh800z cell sorter
    C8 augments important maturation markers specifically in INS-producing β cells. Quantitative RT-PCR analysis was performed to measure the relative expression levels of the β cell lineage markers INS , UCN3 , and GCK and the transcription factors PDX1 , NKX6.1 , and MAFA from sorted Venus+ and NC cells with or without treatment with C8 and/or bFGF. Briefly, #9–15 hiPSCs were differentiated as described above and treated with 5 μM C8 or 5 μM C8 and 50 ng/mL bFGF in the third stage of differentiation. After differentiation, samples were dissociated with TrypLE and sorted with an <t>SH800Z</t> cell sorter (Sony) for Venus+ and NC cells. Fifty cells from each group were sorted into 5 μL FCP reagent, which is a lysate reagent of the QIAGEN Fast Lane cDNA kit. For cDNA synthesis, 2 μL of the samples were used, with 0.5 μL of the 10 μL cDNA samples used for quantitative RT-PCR. Error bars indicate SD, n = 3. *p
    Sh800z Cell Sorter, supplied by Sony, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sh800z cell sorter/product/Sony
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sh800z cell sorter - by Bioz Stars, 2021-04
    86/100 stars
    		  Buy from Supplier
    cell sorter sh800z  (Sony)
    86
    Sony cell sorter sh800z
    C8 augments important maturation markers specifically in INS-producing β cells. Quantitative RT-PCR analysis was performed to measure the relative expression levels of the β cell lineage markers INS , UCN3 , and GCK and the transcription factors PDX1 , NKX6.1 , and MAFA from sorted Venus+ and NC cells with or without treatment with C8 and/or bFGF. Briefly, #9–15 hiPSCs were differentiated as described above and treated with 5 μM C8 or 5 μM C8 and 50 ng/mL bFGF in the third stage of differentiation. After differentiation, samples were dissociated with TrypLE and sorted with an <t>SH800Z</t> cell sorter (Sony) for Venus+ and NC cells. Fifty cells from each group were sorted into 5 μL FCP reagent, which is a lysate reagent of the QIAGEN Fast Lane cDNA kit. For cDNA synthesis, 2 μL of the samples were used, with 0.5 μL of the 10 μL cDNA samples used for quantitative RT-PCR. Error bars indicate SD, n = 3. *p
    Cell Sorter Sh800z, supplied by Sony, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell sorter sh800z/product/Sony
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cell sorter sh800z - by Bioz Stars, 2021-04
    86/100 stars
    		  Buy from Supplier
    flow cytometry  (Sony)
    86
    Sony flow cytometry
    C8 augments important maturation markers specifically in INS-producing β cells. Quantitative RT-PCR analysis was performed to measure the relative expression levels of the β cell lineage markers INS , UCN3 , and GCK and the transcription factors PDX1 , NKX6.1 , and MAFA from sorted Venus+ and NC cells with or without treatment with C8 and/or bFGF. Briefly, #9–15 hiPSCs were differentiated as described above and treated with 5 μM C8 or 5 μM C8 and 50 ng/mL bFGF in the third stage of differentiation. After differentiation, samples were dissociated with TrypLE and sorted with an <t>SH800Z</t> cell sorter (Sony) for Venus+ and NC cells. Fifty cells from each group were sorted into 5 μL FCP reagent, which is a lysate reagent of the QIAGEN Fast Lane cDNA kit. For cDNA synthesis, 2 μL of the samples were used, with 0.5 μL of the 10 μL cDNA samples used for quantitative RT-PCR. Error bars indicate SD, n = 3. *p
    Flow Cytometry, supplied by Sony, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flow cytometry/product/Sony
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    flow cytometry - by Bioz Stars, 2021-04
    86/100 stars
    		  Buy from Supplier
    sony sh800z sorter  (Sony)
    86
    Sony sony sh800z sorter
    C8 augments important maturation markers specifically in INS-producing β cells. Quantitative RT-PCR analysis was performed to measure the relative expression levels of the β cell lineage markers INS , UCN3 , and GCK and the transcription factors PDX1 , NKX6.1 , and MAFA from sorted Venus+ and NC cells with or without treatment with C8 and/or bFGF. Briefly, #9–15 hiPSCs were differentiated as described above and treated with 5 μM C8 or 5 μM C8 and 50 ng/mL bFGF in the third stage of differentiation. After differentiation, samples were dissociated with TrypLE and sorted with an <t>SH800Z</t> cell sorter (Sony) for Venus+ and NC cells. Fifty cells from each group were sorted into 5 μL FCP reagent, which is a lysate reagent of the QIAGEN Fast Lane cDNA kit. For cDNA synthesis, 2 μL of the samples were used, with 0.5 μL of the 10 μL cDNA samples used for quantitative RT-PCR. Error bars indicate SD, n = 3. *p
    Sony Sh800z Sorter, supplied by Sony, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sony sh800z sorter/product/Sony
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sony sh800z sorter - by Bioz Stars, 2021-04
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    C8 augments important maturation markers specifically in INS-producing β cells. Quantitative RT-PCR analysis was performed to measure the relative expression levels of the β cell lineage markers INS , UCN3 , and GCK and the transcription factors PDX1 , NKX6.1 , and MAFA from sorted Venus+ and NC cells with or without treatment with C8 and/or bFGF. Briefly, #9–15 hiPSCs were differentiated as described above and treated with 5 μM C8 or 5 μM C8 and 50 ng/mL bFGF in the third stage of differentiation. After differentiation, samples were dissociated with TrypLE and sorted with an SH800Z cell sorter (Sony) for Venus+ and NC cells. Fifty cells from each group were sorted into 5 μL FCP reagent, which is a lysate reagent of the QIAGEN Fast Lane cDNA kit. For cDNA synthesis, 2 μL of the samples were used, with 0.5 μL of the 10 μL cDNA samples used for quantitative RT-PCR. Error bars indicate SD, n = 3. *p

    Journal: Scientific Reports

    Article Title: An inhibitor of fibroblast growth factor receptor-1 (FGFR1) promotes late-stage terminal differentiation from NGN3+ pancreatic endocrine progenitors

    doi: 10.1038/srep35908

    Figure Lengend Snippet: C8 augments important maturation markers specifically in INS-producing β cells. Quantitative RT-PCR analysis was performed to measure the relative expression levels of the β cell lineage markers INS , UCN3 , and GCK and the transcription factors PDX1 , NKX6.1 , and MAFA from sorted Venus+ and NC cells with or without treatment with C8 and/or bFGF. Briefly, #9–15 hiPSCs were differentiated as described above and treated with 5 μM C8 or 5 μM C8 and 50 ng/mL bFGF in the third stage of differentiation. After differentiation, samples were dissociated with TrypLE and sorted with an SH800Z cell sorter (Sony) for Venus+ and NC cells. Fifty cells from each group were sorted into 5 μL FCP reagent, which is a lysate reagent of the QIAGEN Fast Lane cDNA kit. For cDNA synthesis, 2 μL of the samples were used, with 0.5 μL of the 10 μL cDNA samples used for quantitative RT-PCR. Error bars indicate SD, n = 3. *p

    Article Snippet: The number of Venus- and mCherry-expressing cells was analysed in an SH800Z cell sorter (Sony, Tokyo, Japan).

    Techniques: Quantitative RT-PCR, Expressing