serum total ige levels Search Results


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  • 95
    Thermo Fisher serum total ige levels
    Changes in serum free and total <t>IgE</t> levels over time before and after <t>omalizumab</t> administration. Changes in serum free IgE ( Black up - pointing triangle ) and total IgE ( Black square ) levels are shown before commencing omalizumab (−1 week) to 172 weeks after beginning administration. The longitudinal axis represents the logarithmic expression of serum IgE levels
    Serum Total Ige Levels, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Siemens AG serum total ige level
    Changes in serum free and total <t>IgE</t> levels over time before and after <t>omalizumab</t> administration. Changes in serum free IgE ( Black up - pointing triangle ) and total IgE ( Black square ) levels are shown before commencing omalizumab (−1 week) to 172 weeks after beginning administration. The longitudinal axis represents the logarithmic expression of serum IgE levels
    Serum Total Ige Level, supplied by Siemens AG, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Shibayagi serum ige
    Effect of PG extracts for Total <t>IgE</t> levels in serum and infiltrated the mast cells in the DNCB-induced AD-like skin lesion (A) IgE concentration of each mouse was measured after sacrifice by <t>ELISA.</t> Data are presented as mean ± SEM (n = 5). Normalcontrol (NC), postive control (PC), PG1 (DNCB + PG 300 mg/kg), PG2 (DNCB + PG 500 mg/kg), DEX (DNCB + dexamethasone 1.5 mg/kg). (B) The number of infiltrating mast cells in the tissue sections of the skin lesion was counted in five sites chosen randomly. PG (DNCB + PG 500 mg/ kg). * P
    Serum Ige, supplied by Shibayagi, used in various techniques. Bioz Stars score: 91/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioreclamationIVT ige
    Effect of PG extracts for Total <t>IgE</t> levels in serum and infiltrated the mast cells in the DNCB-induced AD-like skin lesion (A) IgE concentration of each mouse was measured after sacrifice by <t>ELISA.</t> Data are presented as mean ± SEM (n = 5). Normalcontrol (NC), postive control (PC), PG1 (DNCB + PG 300 mg/kg), PG2 (DNCB + PG 500 mg/kg), DEX (DNCB + dexamethasone 1.5 mg/kg). (B) The number of infiltrating mast cells in the tissue sections of the skin lesion was counted in five sites chosen randomly. PG (DNCB + PG 500 mg/ kg). * P
    Ige, supplied by BioreclamationIVT, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Cayman Chemical ova specific ige
    Effect of anti-HMGB1 antibody treatment on <t>OVA-induced</t> chronic airway inflammation and OVA-specific <t>IgE.</t> ( a ) Inflammatory cells in the BALF. ( b ) The level of OVA-specific IgE in serum. ( c ) H E-stained lung tissue (×100). ( d ) The effect of anti-HMGB1 antibody treatment on OVA-induced AHR as assessed by a non-invasive method. ( e ) The effect of anti-HMGB1 antibody treatment on OVA-induced AHR as assessed by an invasive method. PBS: control group; OVA: chronic asthma group; OVA+isotype antibody: OVA+isotype antibody group; OVA+anti-HMGB1: OVA+anti-HMGB1 antibody group. # P
    Ova Specific Ige, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 92/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    BioLegend ova specific ige
    Effects of Epac regulators on airway inflammation and airway hyperresponsiveness in acute asthmatic mice. Female BALB/c mice were sensitized at days 0 and 14 and challenged at days 25–27 in acute asthma group. Mice were received an intratracheal (i.t.). injection of PBS, or an i.t. injection of 25 μg 8-pCPT-2′-O-Me-cAMP (8pCPT), or an intraperitoneal (i.p.). injection of 10 mg/kg ESI-09 30 min before each challenge. Littermates only received allergen challenge as control. Lung tissues were stained with HE and PAS (arrow) (× 200) ( a ). Inflammatory score and PAS-positive areas per unit length were determined ( b ). Total <t>IgE</t> and <t>OVA</t> specific IgE (OVA-IgE) in serum were detected by ELISA ( c ). BALF was analyzed for ( d ) the number of total and different cells (Eos, eosinophils; Lym, lymphocytes; Neu, neutrophils) and ( e ) cytokines (IFN-γ, IL-4, 5 and 13). The frequency of Th1 (CD4 + IFN-γ + ) and Th2 (CD4 + IL-4 + ) cells in lung tissues were detected by FACS ( f ). Lung tissues were measured for transcription factor T-bet and GATA3 mRNA by qPCR ( g ). Airway responsiveness and airway compliance to increased concentration of methacholine (Mch) were measured ( h ). * Control vs Vehicle/Acute Asthma, # Vehicle/Acute Asthma vs ESI-09/Acute Asthma. All data are expressed as mean ± SEM of three independent experiments ( n = 4–6). *** P
    Ova Specific Ige, supplied by BioLegend, used in various techniques. Bioz Stars score: 92/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Bio-Rad ova specific ige
    Intact Th1/Th2 response in Spred-1–deficient mice. (A) Serum concentration of total <t>IgE,</t> <t>OVA-specific</t> IgE, and total IgA in an OVA-induced asthma model. Data are means ± SEM of at least six mice per group. (B) Cytokine production from CD4 + T cells after stimulation with anti-TCR plus anti-CD28 mAbs. CD4 + T cells were isolated from paratracheal and mediastinal lymph node cells from WT and Spred-1–deficient mice after OVA challenges and cultured with anti-TCR plus anti-CD28 mAbs in vitro for 48 h. Cytokine production in the supernatants was assessed by ELISA. All data are means ± SEM. (C) CD4 + T cells from WT and Spred-1–deficient mice were stimulated for 5 d with anti-TCR plus anti-CD28 mAbs in the presence of IL-12 and anti–IL-4 mAb (Th1-skewed condition) or IL-4 and anti–IL-12 mAb (Th2-skewed condition). The generation of Th1 and Th2 cells was assessed by cytoplasmic staining with IL-4 and IFN-γ. The absolute numbers of cells harvested were similar in these cultures. The percentages of cells in each quadrant are shown. Three independent experiments were performed with similar results.
    Ova Specific Ige, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson opteia mouse ige elisa set
    Intact Th1/Th2 response in Spred-1–deficient mice. (A) Serum concentration of total <t>IgE,</t> <t>OVA-specific</t> IgE, and total IgA in an OVA-induced asthma model. Data are means ± SEM of at least six mice per group. (B) Cytokine production from CD4 + T cells after stimulation with anti-TCR plus anti-CD28 mAbs. CD4 + T cells were isolated from paratracheal and mediastinal lymph node cells from WT and Spred-1–deficient mice after OVA challenges and cultured with anti-TCR plus anti-CD28 mAbs in vitro for 48 h. Cytokine production in the supernatants was assessed by ELISA. All data are means ± SEM. (C) CD4 + T cells from WT and Spred-1–deficient mice were stimulated for 5 d with anti-TCR plus anti-CD28 mAbs in the presence of IL-12 and anti–IL-4 mAb (Th1-skewed condition) or IL-4 and anti–IL-12 mAb (Th2-skewed condition). The generation of Th1 and Th2 cells was assessed by cytoplasmic staining with IL-4 and IFN-γ. The absolute numbers of cells harvested were similar in these cultures. The percentages of cells in each quadrant are shown. Three independent experiments were performed with similar results.
    Opteia Mouse Ige Elisa Set, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Becton Dickinson mouse ige elisa kit
    Intact Th1/Th2 response in Spred-1–deficient mice. (A) Serum concentration of total <t>IgE,</t> <t>OVA-specific</t> IgE, and total IgA in an OVA-induced asthma model. Data are means ± SEM of at least six mice per group. (B) Cytokine production from CD4 + T cells after stimulation with anti-TCR plus anti-CD28 mAbs. CD4 + T cells were isolated from paratracheal and mediastinal lymph node cells from WT and Spred-1–deficient mice after OVA challenges and cultured with anti-TCR plus anti-CD28 mAbs in vitro for 48 h. Cytokine production in the supernatants was assessed by ELISA. All data are means ± SEM. (C) CD4 + T cells from WT and Spred-1–deficient mice were stimulated for 5 d with anti-TCR plus anti-CD28 mAbs in the presence of IL-12 and anti–IL-4 mAb (Th1-skewed condition) or IL-4 and anti–IL-12 mAb (Th2-skewed condition). The generation of Th1 and Th2 cells was assessed by cytoplasmic staining with IL-4 and IFN-γ. The absolute numbers of cells harvested were similar in these cultures. The percentages of cells in each quadrant are shown. Three independent experiments were performed with similar results.
    Mouse Ige Elisa Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 89/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson opteia elisa set
    Intact Th1/Th2 response in Spred-1–deficient mice. (A) Serum concentration of total <t>IgE,</t> <t>OVA-specific</t> IgE, and total IgA in an OVA-induced asthma model. Data are means ± SEM of at least six mice per group. (B) Cytokine production from CD4 + T cells after stimulation with anti-TCR plus anti-CD28 mAbs. CD4 + T cells were isolated from paratracheal and mediastinal lymph node cells from WT and Spred-1–deficient mice after OVA challenges and cultured with anti-TCR plus anti-CD28 mAbs in vitro for 48 h. Cytokine production in the supernatants was assessed by ELISA. All data are means ± SEM. (C) CD4 + T cells from WT and Spred-1–deficient mice were stimulated for 5 d with anti-TCR plus anti-CD28 mAbs in the presence of IL-12 and anti–IL-4 mAb (Th1-skewed condition) or IL-4 and anti–IL-12 mAb (Th2-skewed condition). The generation of Th1 and Th2 cells was assessed by cytoplasmic staining with IL-4 and IFN-γ. The absolute numbers of cells harvested were similar in these cultures. The percentages of cells in each quadrant are shown. Three independent experiments were performed with similar results.
    Opteia Elisa Set, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 184 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Cell Signaling Technology Inc stat3 antisera
    Schematic diagram of <t>STAT3</t> protein structure showing the position of the N567D mutation identified from a patient with Hyper IgE syndrome.
    Stat3 Antisera, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Changes in serum free and total IgE levels over time before and after omalizumab administration. Changes in serum free IgE ( Black up - pointing triangle ) and total IgE ( Black square ) levels are shown before commencing omalizumab (−1 week) to 172 weeks after beginning administration. The longitudinal axis represents the logarithmic expression of serum IgE levels

    Journal: Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology

    Article Title: Serum free IgE guided dose reduction of omalizumab: a case report

    doi: 10.1186/s13223-017-0211-z

    Figure Lengend Snippet: Changes in serum free and total IgE levels over time before and after omalizumab administration. Changes in serum free IgE ( Black up - pointing triangle ) and total IgE ( Black square ) levels are shown before commencing omalizumab (−1 week) to 172 weeks after beginning administration. The longitudinal axis represents the logarithmic expression of serum IgE levels

    Article Snippet: Discussion At present, free IgE and IgE-omalizumab complexes cannot be distinguished using current clinical methods for measuring serum total IgE levels, like ImmunoCAP (Thermo Fisher Scientific, Uppsala, Sweden); thus, serum total IgE levels appear to increase following the administration of omalizumab [ , ].

    Techniques: Expressing

    Effect of PG extracts for Total IgE levels in serum and infiltrated the mast cells in the DNCB-induced AD-like skin lesion (A) IgE concentration of each mouse was measured after sacrifice by ELISA. Data are presented as mean ± SEM (n = 5). Normalcontrol (NC), postive control (PC), PG1 (DNCB + PG 300 mg/kg), PG2 (DNCB + PG 500 mg/kg), DEX (DNCB + dexamethasone 1.5 mg/kg). (B) The number of infiltrating mast cells in the tissue sections of the skin lesion was counted in five sites chosen randomly. PG (DNCB + PG 500 mg/ kg). * P

    Journal: Indian Journal of Pharmacology

    Article Title: Platycodon grandiflorus alleviates DNCB-induced atopy-like dermatitis in NC/Nga mice

    doi: 10.4103/0253-7613.99306

    Figure Lengend Snippet: Effect of PG extracts for Total IgE levels in serum and infiltrated the mast cells in the DNCB-induced AD-like skin lesion (A) IgE concentration of each mouse was measured after sacrifice by ELISA. Data are presented as mean ± SEM (n = 5). Normalcontrol (NC), postive control (PC), PG1 (DNCB + PG 300 mg/kg), PG2 (DNCB + PG 500 mg/kg), DEX (DNCB + dexamethasone 1.5 mg/kg). (B) The number of infiltrating mast cells in the tissue sections of the skin lesion was counted in five sites chosen randomly. PG (DNCB + PG 500 mg/ kg). * P

    Article Snippet: The levels of total serum IgE were measured by a mouse IgE ELISA kit (Shibayagi, Japan), following the manufacturer's instructions.

    Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay

    Effect of anti-HMGB1 antibody treatment on OVA-induced chronic airway inflammation and OVA-specific IgE. ( a ) Inflammatory cells in the BALF. ( b ) The level of OVA-specific IgE in serum. ( c ) H E-stained lung tissue (×100). ( d ) The effect of anti-HMGB1 antibody treatment on OVA-induced AHR as assessed by a non-invasive method. ( e ) The effect of anti-HMGB1 antibody treatment on OVA-induced AHR as assessed by an invasive method. PBS: control group; OVA: chronic asthma group; OVA+isotype antibody: OVA+isotype antibody group; OVA+anti-HMGB1: OVA+anti-HMGB1 antibody group. # P

    Journal: Cellular and Molecular Immunology

    Article Title: HMGB1 contributes to allergen-induced airway remodeling in a murine model of chronic asthma by modulating airway inflammation and activating lung fibroblasts

    doi: 10.1038/cmi.2014.60

    Figure Lengend Snippet: Effect of anti-HMGB1 antibody treatment on OVA-induced chronic airway inflammation and OVA-specific IgE. ( a ) Inflammatory cells in the BALF. ( b ) The level of OVA-specific IgE in serum. ( c ) H E-stained lung tissue (×100). ( d ) The effect of anti-HMGB1 antibody treatment on OVA-induced AHR as assessed by a non-invasive method. ( e ) The effect of anti-HMGB1 antibody treatment on OVA-induced AHR as assessed by an invasive method. PBS: control group; OVA: chronic asthma group; OVA+isotype antibody: OVA+isotype antibody group; OVA+anti-HMGB1: OVA+anti-HMGB1 antibody group. # P

    Article Snippet: The serum levels of total and OVA-specific IgE were determined using an Anti-Ovalbumin IgE (mouse) EIA Kit (Cayman Chemical, Michigan, USA).

    Techniques: Staining

    Effects of Epac regulators on airway inflammation and airway hyperresponsiveness in acute asthmatic mice. Female BALB/c mice were sensitized at days 0 and 14 and challenged at days 25–27 in acute asthma group. Mice were received an intratracheal (i.t.). injection of PBS, or an i.t. injection of 25 μg 8-pCPT-2′-O-Me-cAMP (8pCPT), or an intraperitoneal (i.p.). injection of 10 mg/kg ESI-09 30 min before each challenge. Littermates only received allergen challenge as control. Lung tissues were stained with HE and PAS (arrow) (× 200) ( a ). Inflammatory score and PAS-positive areas per unit length were determined ( b ). Total IgE and OVA specific IgE (OVA-IgE) in serum were detected by ELISA ( c ). BALF was analyzed for ( d ) the number of total and different cells (Eos, eosinophils; Lym, lymphocytes; Neu, neutrophils) and ( e ) cytokines (IFN-γ, IL-4, 5 and 13). The frequency of Th1 (CD4 + IFN-γ + ) and Th2 (CD4 + IL-4 + ) cells in lung tissues were detected by FACS ( f ). Lung tissues were measured for transcription factor T-bet and GATA3 mRNA by qPCR ( g ). Airway responsiveness and airway compliance to increased concentration of methacholine (Mch) were measured ( h ). * Control vs Vehicle/Acute Asthma, # Vehicle/Acute Asthma vs ESI-09/Acute Asthma. All data are expressed as mean ± SEM of three independent experiments ( n = 4–6). *** P

    Journal: Respiratory Research

    Article Title: Exchange protein directly activated by cAMP (Epac) protects against airway inflammation and airway remodeling in asthmatic mice

    doi: 10.1186/s12931-019-1260-2

    Figure Lengend Snippet: Effects of Epac regulators on airway inflammation and airway hyperresponsiveness in acute asthmatic mice. Female BALB/c mice were sensitized at days 0 and 14 and challenged at days 25–27 in acute asthma group. Mice were received an intratracheal (i.t.). injection of PBS, or an i.t. injection of 25 μg 8-pCPT-2′-O-Me-cAMP (8pCPT), or an intraperitoneal (i.p.). injection of 10 mg/kg ESI-09 30 min before each challenge. Littermates only received allergen challenge as control. Lung tissues were stained with HE and PAS (arrow) (× 200) ( a ). Inflammatory score and PAS-positive areas per unit length were determined ( b ). Total IgE and OVA specific IgE (OVA-IgE) in serum were detected by ELISA ( c ). BALF was analyzed for ( d ) the number of total and different cells (Eos, eosinophils; Lym, lymphocytes; Neu, neutrophils) and ( e ) cytokines (IFN-γ, IL-4, 5 and 13). The frequency of Th1 (CD4 + IFN-γ + ) and Th2 (CD4 + IL-4 + ) cells in lung tissues were detected by FACS ( f ). Lung tissues were measured for transcription factor T-bet and GATA3 mRNA by qPCR ( g ). Airway responsiveness and airway compliance to increased concentration of methacholine (Mch) were measured ( h ). * Control vs Vehicle/Acute Asthma, # Vehicle/Acute Asthma vs ESI-09/Acute Asthma. All data are expressed as mean ± SEM of three independent experiments ( n = 4–6). *** P

    Article Snippet: Effects of 8pCPT and ESI-09 on total and OVA-specific IgE in serum The levels of total and OVA-specific IgE in serum were measured by ELISA.

    Techniques: Mouse Assay, Injection, Staining, Enzyme-linked Immunosorbent Assay, FACS, Real-time Polymerase Chain Reaction, Concentration Assay

    Intact Th1/Th2 response in Spred-1–deficient mice. (A) Serum concentration of total IgE, OVA-specific IgE, and total IgA in an OVA-induced asthma model. Data are means ± SEM of at least six mice per group. (B) Cytokine production from CD4 + T cells after stimulation with anti-TCR plus anti-CD28 mAbs. CD4 + T cells were isolated from paratracheal and mediastinal lymph node cells from WT and Spred-1–deficient mice after OVA challenges and cultured with anti-TCR plus anti-CD28 mAbs in vitro for 48 h. Cytokine production in the supernatants was assessed by ELISA. All data are means ± SEM. (C) CD4 + T cells from WT and Spred-1–deficient mice were stimulated for 5 d with anti-TCR plus anti-CD28 mAbs in the presence of IL-12 and anti–IL-4 mAb (Th1-skewed condition) or IL-4 and anti–IL-12 mAb (Th2-skewed condition). The generation of Th1 and Th2 cells was assessed by cytoplasmic staining with IL-4 and IFN-γ. The absolute numbers of cells harvested were similar in these cultures. The percentages of cells in each quadrant are shown. Three independent experiments were performed with similar results.

    Journal: The Journal of Experimental Medicine

    Article Title: Spred-1 negatively regulates allergen-induced airway eosinophilia and hyperresponsiveness

    doi: 10.1084/jem.20040616

    Figure Lengend Snippet: Intact Th1/Th2 response in Spred-1–deficient mice. (A) Serum concentration of total IgE, OVA-specific IgE, and total IgA in an OVA-induced asthma model. Data are means ± SEM of at least six mice per group. (B) Cytokine production from CD4 + T cells after stimulation with anti-TCR plus anti-CD28 mAbs. CD4 + T cells were isolated from paratracheal and mediastinal lymph node cells from WT and Spred-1–deficient mice after OVA challenges and cultured with anti-TCR plus anti-CD28 mAbs in vitro for 48 h. Cytokine production in the supernatants was assessed by ELISA. All data are means ± SEM. (C) CD4 + T cells from WT and Spred-1–deficient mice were stimulated for 5 d with anti-TCR plus anti-CD28 mAbs in the presence of IL-12 and anti–IL-4 mAb (Th1-skewed condition) or IL-4 and anti–IL-12 mAb (Th2-skewed condition). The generation of Th1 and Th2 cells was assessed by cytoplasmic staining with IL-4 and IFN-γ. The absolute numbers of cells harvested were similar in these cultures. The percentages of cells in each quadrant are shown. Three independent experiments were performed with similar results.

    Article Snippet: The serum levels of total and OVA-specific IgE were analyzed by ELISA with rat anti–mouse IgE (Serotec Ltd.).

    Techniques: Mouse Assay, Concentration Assay, Isolation, Cell Culture, In Vitro, Enzyme-linked Immunosorbent Assay, Staining

    Schematic diagram of STAT3 protein structure showing the position of the N567D mutation identified from a patient with Hyper IgE syndrome.

    Journal: Case Reports in Immunology

    Article Title: Evidence that a STAT3 Mutation Causing Hyper IgE Syndrome Leads to Repression of Transcriptional Activity

    doi: 10.1155/2019/1869524

    Figure Lengend Snippet: Schematic diagram of STAT3 protein structure showing the position of the N567D mutation identified from a patient with Hyper IgE syndrome.

    Article Snippet: Membranes were probed with STAT3 antisera (1 : 1000; Cell Signalling, Beverly, MA, USA) or beta-actin (1 : 2000; Sigma-Aldrich, Poole, Dorset, UK), and then with appropriate alkaline phosphatase conjugated secondary antibodies.

    Techniques: Mutagenesis

    Transfection of N567D-STAT3 into cells increased STAT3 activity. (a) HEK293 cells were transfected with equal amounts of an empty vector, wildtype STAT3 or N567D and expression of STAT3 was studied by Western blotting. Results are representative of two independent experiments. (b) Cells were alternatively transfected with wildtype, N567D, and mutations which are known to activate (Y640F), and inactivate (V637M) STAT3. These cells were either grown in the presence or absence of 20 ng/ml IL-6 for 18 h, and transcriptional activity was determined using a dual-luciferase reporter assay ( n = 3 − 6). ∗ P

    Journal: Case Reports in Immunology

    Article Title: Evidence that a STAT3 Mutation Causing Hyper IgE Syndrome Leads to Repression of Transcriptional Activity

    doi: 10.1155/2019/1869524

    Figure Lengend Snippet: Transfection of N567D-STAT3 into cells increased STAT3 activity. (a) HEK293 cells were transfected with equal amounts of an empty vector, wildtype STAT3 or N567D and expression of STAT3 was studied by Western blotting. Results are representative of two independent experiments. (b) Cells were alternatively transfected with wildtype, N567D, and mutations which are known to activate (Y640F), and inactivate (V637M) STAT3. These cells were either grown in the presence or absence of 20 ng/ml IL-6 for 18 h, and transcriptional activity was determined using a dual-luciferase reporter assay ( n = 3 − 6). ∗ P

    Article Snippet: Membranes were probed with STAT3 antisera (1 : 1000; Cell Signalling, Beverly, MA, USA) or beta-actin (1 : 2000; Sigma-Aldrich, Poole, Dorset, UK), and then with appropriate alkaline phosphatase conjugated secondary antibodies.

    Techniques: Transfection, Activity Assay, Plasmid Preparation, Expressing, Western Blot, Luciferase, Reporter Assay