sequencing primer Search Results


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  • 99
    New England Biolabs nebnext multiplex oligos for illumina 96 unique dual index primer pairs set 2
    Nebnext Multiplex Oligos For Illumina 96 Unique Dual Index Primer Pairs Set 2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher t7 promoter
    Generation and characterization of a13 −/− zebrafish using CRISPR/Cas9. (A) An oligonucleotide consisting of a <t>T7</t> promoter, a gene-specific target sequence (targeting the signal peptide coding region of zebrafish a13 ), and a guide RNA (gRNA) scaffold sequence was annealed and extended with the gRNA core sequence and then transcribed into a gRNA. (B) Sanger sequencing identified the wt (top) and 8-bp deletion (boxed) in a13 −/− F2 progeny zebrafish (bottom, arrowhead). (C) Western blot by a capillary-based WES system demonstrates the absence ( a13 −/− ) and the presence ( wt ) of a13 protein (~220 kDa) in the lysate of zebrafish embryos (5 days post-fertilization) and in the plasma of 3-month old zebrafish, respectively. (D) A FRETS-VWF73 assay showed normal cleaving activity in the wt adult zebrafish plasma but not the a13 −/− . Such proteolytic activity was abrogated by the addition of 10 mmol/L EDTA. Pooled normal human plasma (NHP) was used as a positive control. Data represent the means ± standard error of mean from three independent experiments.
    T7 Promoter, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4692 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher primer sequences
    Generation and characterization of a13 −/− zebrafish using CRISPR/Cas9. (A) An oligonucleotide consisting of a <t>T7</t> promoter, a gene-specific target sequence (targeting the signal peptide coding region of zebrafish a13 ), and a guide RNA (gRNA) scaffold sequence was annealed and extended with the gRNA core sequence and then transcribed into a gRNA. (B) Sanger sequencing identified the wt (top) and 8-bp deletion (boxed) in a13 −/− F2 progeny zebrafish (bottom, arrowhead). (C) Western blot by a capillary-based WES system demonstrates the absence ( a13 −/− ) and the presence ( wt ) of a13 protein (~220 kDa) in the lysate of zebrafish embryos (5 days post-fertilization) and in the plasma of 3-month old zebrafish, respectively. (D) A FRETS-VWF73 assay showed normal cleaving activity in the wt adult zebrafish plasma but not the a13 −/− . Such proteolytic activity was abrogated by the addition of 10 mmol/L EDTA. Pooled normal human plasma (NHP) was used as a positive control. Data represent the means ± standard error of mean from three independent experiments.
    Primer Sequences, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 9698 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Sangon Biotech primer sequences
    Generation and characterization of a13 −/− zebrafish using CRISPR/Cas9. (A) An oligonucleotide consisting of a <t>T7</t> promoter, a gene-specific target sequence (targeting the signal peptide coding region of zebrafish a13 ), and a guide RNA (gRNA) scaffold sequence was annealed and extended with the gRNA core sequence and then transcribed into a gRNA. (B) Sanger sequencing identified the wt (top) and 8-bp deletion (boxed) in a13 −/− F2 progeny zebrafish (bottom, arrowhead). (C) Western blot by a capillary-based WES system demonstrates the absence ( a13 −/− ) and the presence ( wt ) of a13 protein (~220 kDa) in the lysate of zebrafish embryos (5 days post-fertilization) and in the plasma of 3-month old zebrafish, respectively. (D) A FRETS-VWF73 assay showed normal cleaving activity in the wt adult zebrafish plasma but not the a13 −/− . Such proteolytic activity was abrogated by the addition of 10 mmol/L EDTA. Pooled normal human plasma (NHP) was used as a positive control. Data represent the means ± standard error of mean from three independent experiments.
    Primer Sequences, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 94/100, based on 2001 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Bio-Rad primer sequences
    Generation and characterization of a13 −/− zebrafish using CRISPR/Cas9. (A) An oligonucleotide consisting of a <t>T7</t> promoter, a gene-specific target sequence (targeting the signal peptide coding region of zebrafish a13 ), and a guide RNA (gRNA) scaffold sequence was annealed and extended with the gRNA core sequence and then transcribed into a gRNA. (B) Sanger sequencing identified the wt (top) and 8-bp deletion (boxed) in a13 −/− F2 progeny zebrafish (bottom, arrowhead). (C) Western blot by a capillary-based WES system demonstrates the absence ( a13 −/− ) and the presence ( wt ) of a13 protein (~220 kDa) in the lysate of zebrafish embryos (5 days post-fertilization) and in the plasma of 3-month old zebrafish, respectively. (D) A FRETS-VWF73 assay showed normal cleaving activity in the wt adult zebrafish plasma but not the a13 −/− . Such proteolytic activity was abrogated by the addition of 10 mmol/L EDTA. Pooled normal human plasma (NHP) was used as a positive control. Data represent the means ± standard error of mean from three independent experiments.
    Primer Sequences, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1431 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc primer sequences
    Generation and characterization of a13 −/− zebrafish using CRISPR/Cas9. (A) An oligonucleotide consisting of a <t>T7</t> promoter, a gene-specific target sequence (targeting the signal peptide coding region of zebrafish a13 ), and a guide RNA (gRNA) scaffold sequence was annealed and extended with the gRNA core sequence and then transcribed into a gRNA. (B) Sanger sequencing identified the wt (top) and 8-bp deletion (boxed) in a13 −/− F2 progeny zebrafish (bottom, arrowhead). (C) Western blot by a capillary-based WES system demonstrates the absence ( a13 −/− ) and the presence ( wt ) of a13 protein (~220 kDa) in the lysate of zebrafish embryos (5 days post-fertilization) and in the plasma of 3-month old zebrafish, respectively. (D) A FRETS-VWF73 assay showed normal cleaving activity in the wt adult zebrafish plasma but not the a13 −/− . Such proteolytic activity was abrogated by the addition of 10 mmol/L EDTA. Pooled normal human plasma (NHP) was used as a positive control. Data represent the means ± standard error of mean from three independent experiments.
    Primer Sequences, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 851 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sequencing primer
    Generation and characterization of a13 −/− zebrafish using CRISPR/Cas9. (A) An oligonucleotide consisting of a <t>T7</t> promoter, a gene-specific target sequence (targeting the signal peptide coding region of zebrafish a13 ), and a guide RNA (gRNA) scaffold sequence was annealed and extended with the gRNA core sequence and then transcribed into a gRNA. (B) Sanger sequencing identified the wt (top) and 8-bp deletion (boxed) in a13 −/− F2 progeny zebrafish (bottom, arrowhead). (C) Western blot by a capillary-based WES system demonstrates the absence ( a13 −/− ) and the presence ( wt ) of a13 protein (~220 kDa) in the lysate of zebrafish embryos (5 days post-fertilization) and in the plasma of 3-month old zebrafish, respectively. (D) A FRETS-VWF73 assay showed normal cleaving activity in the wt adult zebrafish plasma but not the a13 −/− . Such proteolytic activity was abrogated by the addition of 10 mmol/L EDTA. Pooled normal human plasma (NHP) was used as a positive control. Data represent the means ± standard error of mean from three independent experiments.
    Sequencing Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 807 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc illumina sequencing primer
    Experimental design to identify serum resistance genes in EC958. (A) Selection steps employed using fresh serum as test and inactivated serum as control. (B) Schematic illustration of the <t>Illumina</t> sequencing procedure including the use of a custom oligo for indexing and enrichment of insert sites.
    Illumina Sequencing Primer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 742 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Biotechnology Information primer sequences
    Experimental design to identify serum resistance genes in EC958. (A) Selection steps employed using fresh serum as test and inactivated serum as control. (B) Schematic illustration of the <t>Illumina</t> sequencing procedure including the use of a custom oligo for indexing and enrichment of insert sites.
    Primer Sequences, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 93/100, based on 901 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Eurofins primer sequences
    Experimental design to identify serum resistance genes in EC958. (A) Selection steps employed using fresh serum as test and inactivated serum as control. (B) Schematic illustration of the <t>Illumina</t> sequencing procedure including the use of a custom oligo for indexing and enrichment of insert sites.
    Primer Sequences, supplied by Eurofins, used in various techniques. Bioz Stars score: 93/100, based on 530 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The Jackson Laboratory primer sequences
    Experimental design to identify serum resistance genes in EC958. (A) Selection steps employed using fresh serum as test and inactivated serum as control. (B) Schematic illustration of the <t>Illumina</t> sequencing procedure including the use of a custom oligo for indexing and enrichment of insert sites.
    Primer Sequences, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 93/100, based on 384 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Shanghai Generay Biotech primer sequences
    Experimental design to identify serum resistance genes in EC958. (A) Selection steps employed using fresh serum as test and inactivated serum as control. (B) Schematic illustration of the <t>Illumina</t> sequencing procedure including the use of a custom oligo for indexing and enrichment of insert sites.
    Primer Sequences, supplied by Shanghai Generay Biotech, used in various techniques. Bioz Stars score: 92/100, based on 313 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    BIOTAGE sequencing primer
    Experimental design to identify serum resistance genes in EC958. (A) Selection steps employed using fresh serum as test and inactivated serum as control. (B) Schematic illustration of the <t>Illumina</t> sequencing procedure including the use of a custom oligo for indexing and enrichment of insert sites.
    Sequencing Primer, supplied by BIOTAGE, used in various techniques. Bioz Stars score: 92/100, based on 350 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher sp6 promoter
    Experimental design to identify serum resistance genes in EC958. (A) Selection steps employed using fresh serum as test and inactivated serum as control. (B) Schematic illustration of the <t>Illumina</t> sequencing procedure including the use of a custom oligo for indexing and enrichment of insert sites.
    Sp6 Promoter, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 795 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare thermo sequenase fluorescent labeled primer cycle sequencing kit
    Experimental design to identify serum resistance genes in EC958. (A) Selection steps employed using fresh serum as test and inactivated serum as control. (B) Schematic illustration of the <t>Illumina</t> sequencing procedure including the use of a custom oligo for indexing and enrichment of insert sites.
    Thermo Sequenase Fluorescent Labeled Primer Cycle Sequencing Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 96/100, based on 459 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Pacific Biosciences sequencing primer
    Experimental design to identify serum resistance genes in EC958. (A) Selection steps employed using fresh serum as test and inactivated serum as control. (B) Schematic illustration of the <t>Illumina</t> sequencing procedure including the use of a custom oligo for indexing and enrichment of insert sites.
    Sequencing Primer, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 237 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher m13 primer
    Representative <t>M13</t> PCR profiles of Candida isolates amplified with the M13 core sequence. A negative image is shown. Lanes M, 100-bp ladder, with sizes indicated on the left; lanes 1 to 24, Candida isolates 332T1/97*, 135BM2/94, 135MX2/94, 135MX1/94, 372RB/94, 870/99, WK1/93, WK2/00, 689/99, 460(1)/98*, DW1/93, 80/99, PTR/94, 688/94, 211/00, 455rgh/94, 455sm/94, PB1/93, 372F/94, 91R/94, 196D/94, 684/93**, 458L/94**, and 965L/97*. ∗, atypical C. albicans profile; ∗∗, non- C. albicans .
    M13 Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 545 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Kaneka Corp primer sequences
    Representative <t>M13</t> PCR profiles of Candida isolates amplified with the M13 core sequence. A negative image is shown. Lanes M, 100-bp ladder, with sizes indicated on the left; lanes 1 to 24, Candida isolates 332T1/97*, 135BM2/94, 135MX2/94, 135MX1/94, 372RB/94, 870/99, WK1/93, WK2/00, 689/99, 460(1)/98*, DW1/93, 80/99, PTR/94, 688/94, 211/00, 455rgh/94, 455sm/94, PB1/93, 372F/94, 91R/94, 196D/94, 684/93**, 458L/94**, and 965L/97*. ∗, atypical C. albicans profile; ∗∗, non- C. albicans .
    Primer Sequences, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 93/100, based on 177 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PrimerDesign Inc primer sequences
    Representative <t>M13</t> PCR profiles of Candida isolates amplified with the M13 core sequence. A negative image is shown. Lanes M, 100-bp ladder, with sizes indicated on the left; lanes 1 to 24, Candida isolates 332T1/97*, 135BM2/94, 135MX2/94, 135MX1/94, 372RB/94, 870/99, WK1/93, WK2/00, 689/99, 460(1)/98*, DW1/93, 80/99, PTR/94, 688/94, 211/00, 455rgh/94, 455sm/94, PB1/93, 372F/94, 91R/94, 196D/94, 684/93**, 458L/94**, and 965L/97*. ∗, atypical C. albicans profile; ∗∗, non- C. albicans .
    Primer Sequences, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 93/100, based on 327 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Generation and characterization of a13 −/− zebrafish using CRISPR/Cas9. (A) An oligonucleotide consisting of a T7 promoter, a gene-specific target sequence (targeting the signal peptide coding region of zebrafish a13 ), and a guide RNA (gRNA) scaffold sequence was annealed and extended with the gRNA core sequence and then transcribed into a gRNA. (B) Sanger sequencing identified the wt (top) and 8-bp deletion (boxed) in a13 −/− F2 progeny zebrafish (bottom, arrowhead). (C) Western blot by a capillary-based WES system demonstrates the absence ( a13 −/− ) and the presence ( wt ) of a13 protein (~220 kDa) in the lysate of zebrafish embryos (5 days post-fertilization) and in the plasma of 3-month old zebrafish, respectively. (D) A FRETS-VWF73 assay showed normal cleaving activity in the wt adult zebrafish plasma but not the a13 −/− . Such proteolytic activity was abrogated by the addition of 10 mmol/L EDTA. Pooled normal human plasma (NHP) was used as a positive control. Data represent the means ± standard error of mean from three independent experiments.

    Journal: Haematologica

    Article Title: Histone-induced thrombotic thrombocytopenic purpura in adamts13−/− zebrafish depends on von Willebrand factor

    doi: 10.3324/haematol.2019.237396

    Figure Lengend Snippet: Generation and characterization of a13 −/− zebrafish using CRISPR/Cas9. (A) An oligonucleotide consisting of a T7 promoter, a gene-specific target sequence (targeting the signal peptide coding region of zebrafish a13 ), and a guide RNA (gRNA) scaffold sequence was annealed and extended with the gRNA core sequence and then transcribed into a gRNA. (B) Sanger sequencing identified the wt (top) and 8-bp deletion (boxed) in a13 −/− F2 progeny zebrafish (bottom, arrowhead). (C) Western blot by a capillary-based WES system demonstrates the absence ( a13 −/− ) and the presence ( wt ) of a13 protein (~220 kDa) in the lysate of zebrafish embryos (5 days post-fertilization) and in the plasma of 3-month old zebrafish, respectively. (D) A FRETS-VWF73 assay showed normal cleaving activity in the wt adult zebrafish plasma but not the a13 −/− . Such proteolytic activity was abrogated by the addition of 10 mmol/L EDTA. Pooled normal human plasma (NHP) was used as a positive control. Data represent the means ± standard error of mean from three independent experiments.

    Article Snippet: A 69-nt oligonucleotide, consisting of a T7 promoter, a target sequence, and a gRNA scaffold, was synthesized (ThermoFisher, Waltham, MA, USA).

    Techniques: CRISPR, Sequencing, Western Blot, Activity Assay, Positive Control

    Plasmid maps of HCMV IL-10 (pAZ1c; A) and EBV (pGA6; B) expression vectors are depicted.  The artificial transporter consists of the  E. coli ompF  signal sequence fused in frame to  E. coli  codon optimized mature viral IL-10 genes under control of the T7 promoter. For subcloning, the constructs are flanked by  Eco RI restriction sites. Plasmid pGA6 contains a ColE1, pAZ1c a pUC19-derived pMB1 origin of replication.

    Journal: BMC Biotechnology

    Article Title: Secretory expression of biologically active human Herpes virus interleukin-10 analogues in Escherichia coli via a modified Sec-dependent transporter construct

    doi: 10.1186/1472-6750-13-82

    Figure Lengend Snippet: Plasmid maps of HCMV IL-10 (pAZ1c; A) and EBV (pGA6; B) expression vectors are depicted. The artificial transporter consists of the E. coli ompF signal sequence fused in frame to E. coli codon optimized mature viral IL-10 genes under control of the T7 promoter. For subcloning, the constructs are flanked by Eco RI restriction sites. Plasmid pGA6 contains a ColE1, pAZ1c a pUC19-derived pMB1 origin of replication.

    Article Snippet: The complete sequence of the artifical vIL-10 transporters including the T7 promoter sequence was synthesized by GeneArt (Regensburg, Germany) and cloned into a pUC-derived plasmid with ColE1 origin resulting in the plasmids pGA4 encoding the HCMV IL-10 transporter and pGA6 carrying the EBV IL-10 construct, respectively.

    Techniques: Plasmid Preparation, Expressing, Sequencing, Subcloning, Construct, Derivative Assay

    NGS allows the identification of aptamer motifs and their evolution during SELEX (A) Workflow of the SELEX technique and variations applied in this work. The ssDNA library (N40) was incubated with the HMG-box proteins immobilized in magnetic beads. After incubation, a magnet is used to separate the ssDNA molecules that bound the proteins on the magnetic beads. To amplify the bound molecules a PCR using a modified reverse primer with the T7 promoter region is performed. After purification, in vitro transcription is carried out, followed by in vitro reverse transcription. Finally, an enriched ssDNA library is generated and used for a next cycle or for sequencing. (B) Progression of enrichment along steps of selection as evaluated by the DiVE technique [ 22 ]. The ratio between non-degraded DNA (enriched)/total DNA was calculated from image quantifications of the resulting agarose gel using the ImageJ software [ 39 ] (see material and methods). Rounds 4, 6, 8, 10 and 14 were chosen for NGS analysis. (C) Scheme depicting the workflow for sequence analysis. (D) MEME (The MEME Suite) was used to identify the most characteristic motifs in each sequenced SELEX cycle. Three motifs were the most represented in cycles 8 (motif 3), 10 (motif 2), and 14 (motif 1). Sequences from cycles 8 and 10 share a conserved region inside motifs 3 and 2, whereas this region is lost in motif 1. No representative motif was detected in cycle 4. (E) Dynamics of aptamer motifs along SELEX cycles.

    Journal: bioRxiv

    Article Title: DNA aptamers for the recognition of HMGB1 from Plasmodium falciparum

    doi: 10.1101/528778

    Figure Lengend Snippet: NGS allows the identification of aptamer motifs and their evolution during SELEX (A) Workflow of the SELEX technique and variations applied in this work. The ssDNA library (N40) was incubated with the HMG-box proteins immobilized in magnetic beads. After incubation, a magnet is used to separate the ssDNA molecules that bound the proteins on the magnetic beads. To amplify the bound molecules a PCR using a modified reverse primer with the T7 promoter region is performed. After purification, in vitro transcription is carried out, followed by in vitro reverse transcription. Finally, an enriched ssDNA library is generated and used for a next cycle or for sequencing. (B) Progression of enrichment along steps of selection as evaluated by the DiVE technique [ 22 ]. The ratio between non-degraded DNA (enriched)/total DNA was calculated from image quantifications of the resulting agarose gel using the ImageJ software [ 39 ] (see material and methods). Rounds 4, 6, 8, 10 and 14 were chosen for NGS analysis. (C) Scheme depicting the workflow for sequence analysis. (D) MEME (The MEME Suite) was used to identify the most characteristic motifs in each sequenced SELEX cycle. Three motifs were the most represented in cycles 8 (motif 3), 10 (motif 2), and 14 (motif 1). Sequences from cycles 8 and 10 share a conserved region inside motifs 3 and 2, whereas this region is lost in motif 1. No representative motif was detected in cycle 4. (E) Dynamics of aptamer motifs along SELEX cycles.

    Article Snippet: For transcription-mediated amplification of libraries, a T7 promoter sequence was added (T7 reverse primer: 5’ TTC AGG TAA TAC GAC TCA CTA TAG GGT CAA GTG GTC ATG TAC TAG TCA A 3’).

    Techniques: Next-Generation Sequencing, Incubation, Magnetic Beads, Polymerase Chain Reaction, Modification, Purification, In Vitro, Generated, Sequencing, Selection, Agarose Gel Electrophoresis, Software

    Experimental design to identify serum resistance genes in EC958. (A) Selection steps employed using fresh serum as test and inactivated serum as control. (B) Schematic illustration of the Illumina sequencing procedure including the use of a custom oligo for indexing and enrichment of insert sites.

    Journal: PLoS Genetics

    Article Title: The Serum Resistome of a Globally Disseminated Multidrug Resistant Uropathogenic Escherichia coli Clone

    doi: 10.1371/journal.pgen.1003834

    Figure Lengend Snippet: Experimental design to identify serum resistance genes in EC958. (A) Selection steps employed using fresh serum as test and inactivated serum as control. (B) Schematic illustration of the Illumina sequencing procedure including the use of a custom oligo for indexing and enrichment of insert sites.

    Article Snippet: This oligonucleotide incorporates the Illumina sequencing primer-binding site into transposon specific DNA fragments, enabling the use of the standard Illumina sequencing primer and eliminating the need to design and optimize another sequencing primer for each new transposon sequence.

    Techniques: Selection, Sequencing

    Representative M13 PCR profiles of Candida isolates amplified with the M13 core sequence. A negative image is shown. Lanes M, 100-bp ladder, with sizes indicated on the left; lanes 1 to 24, Candida isolates 332T1/97*, 135BM2/94, 135MX2/94, 135MX1/94, 372RB/94, 870/99, WK1/93, WK2/00, 689/99, 460(1)/98*, DW1/93, 80/99, PTR/94, 688/94, 211/00, 455rgh/94, 455sm/94, PB1/93, 372F/94, 91R/94, 196D/94, 684/93**, 458L/94**, and 965L/97*. ∗, atypical C. albicans profile; ∗∗, non- C. albicans .

    Journal: Journal of Clinical Microbiology

    Article Title: PCR Fingerprinting of Candida albicans Associated with Chronic Hyperplastic Candidosis and Other Oral Conditions

    doi: 10.1128/JCM.39.11.4066-4075.2001

    Figure Lengend Snippet: Representative M13 PCR profiles of Candida isolates amplified with the M13 core sequence. A negative image is shown. Lanes M, 100-bp ladder, with sizes indicated on the left; lanes 1 to 24, Candida isolates 332T1/97*, 135BM2/94, 135MX2/94, 135MX1/94, 372RB/94, 870/99, WK1/93, WK2/00, 689/99, 460(1)/98*, DW1/93, 80/99, PTR/94, 688/94, 211/00, 455rgh/94, 455sm/94, PB1/93, 372F/94, 91R/94, 196D/94, 684/93**, 458L/94**, and 965L/97*. ∗, atypical C. albicans profile; ∗∗, non- C. albicans .

    Article Snippet: Amplification reactions (50 μl) consisted of 5 μl of candidal DNA extract, 10 mM Tris-HCl (pH 9.0), 50 mM potassium chloride, 0.1% Triton X-100, 1.5 mM magnesium chloride, 0.2 mM (each) dNTPs (Roche Diagnostics Ltd.), 3 mM magnesium acetate, 2.5 U of Taq polymerase (Promega), and 30 ng of M13 primer (5′-GAG GGT GGC GGT TCT-3′; Life Technologies Ltd.).

    Techniques: Polymerase Chain Reaction, Amplification, Sequencing