Journal: Scientific Reports
Article Title: Microbial embryonal colonization during pipefish male pregnancy
Figure Lengend Snippet: Sampling overview. Swab samples were taken from ovaries dissected out of pipefish ( Syngnathus typhle ) females (Gonads-GO); from the brood pouch tissue of non-pregnant pipefish males (Non-Pregnant-NP); on the surface of eggs and embryos inside the brood pouch of pregnant males in increasing developmental stages: early pregnancy (EP) circa (1–2 weeks); mid pregnant (MP) (2–4 weeks); late pregnant (LP) (4–6 weeks). In total 20 females, 20 males per pregnancy stage (3*20), and 40 non-pregnant males were used for the study (120 fish). For each group half of the individuals were intraperitoneally injected with heat-killed bacteria suspension ( Vibrio spp. and Tenacibaculum maritimum ) to trigger a parental immune transfer (injected with bacteria solution vs. non-injected). Microbiota samples were taken with sterile swabs by scratching the mucus from the surface of ovaries, brood pouch tissue and embryos inside the paternal brood pouch. Microbiota analysis was performed by Illumina Miseq Sequencing.
Article Snippet: Distinct microbiomes in developmental stages, depending on parental immunological status In total, 994,755 16S rRNA sequences were retained from Illumina Miseq sequencing platform after merging and quality control.
Techniques: Sampling, Fluorescence In Situ Hybridization, Injection, Sequencing