sequant zic-philic column Search Results


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  • 92
    The Nest Group sequant zic philic column
    Sequant Zic Philic Column, supplied by The Nest Group, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co sequant zic philic column
    Sequant Zic Philic Column, supplied by Merck & Co, used in various techniques. Bioz Stars score: 89/100, based on 134 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore sequant zic philic column
    Sequant Zic Philic Column, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA sequant zic philic column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Sequant Zic Philic Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 89/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA sequant zic philic polymeric column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Sequant Zic Philic Polymeric Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co sequant zic philic guard column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Sequant Zic Philic Guard Column, supplied by Merck & Co, used in various techniques. Bioz Stars score: 88/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co 150 mm sequant zic philic column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    150 Mm Sequant Zic Philic Column, supplied by Merck & Co, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore sequant zic philic polymeric column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Sequant Zic Philic Polymeric Column, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore sequant zic philic guard column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Sequant Zic Philic Guard Column, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co sequant zic philic polymeric column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Sequant Zic Philic Polymeric Column, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA sequant zic philic guard column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Sequant Zic Philic Guard Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore instrumentation a sequant zic philic column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Instrumentation A Sequant Zic Philic Column, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA sequant zic philic hplc column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Sequant Zic Philic Hplc Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore sequant emd millipore zic philic column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Sequant Emd Millipore Zic Philic Column, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co sequant zic philic hplc column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Sequant Zic Philic Hplc Column, supplied by Merck & Co, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA sequant zic philic peek coated column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Sequant Zic Philic Peek Coated Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA silica based sequant zic philic column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Silica Based Sequant Zic Philic Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA sequant zic phillic column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Sequant Zic Phillic Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co zicphilic sequant column form merck
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Zicphilic Sequant Column Form Merck, supplied by Merck & Co, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a SeQuant ZIC-pHILIC column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.

    Journal: Frontiers in Microbiology

    Article Title: Identification and Functional Characterization of Small Alarmone Synthetases in Corynebacterium glutamicum

    doi: 10.3389/fmicb.2017.01601

    Figure Lengend Snippet: In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a SeQuant ZIC-pHILIC column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.

    Article Snippet: HPLC analysis of all assay reactions was performed using a LaChrome ULTRA system (HITACHI) and a SeQuant ZIC-pHILIC column (Merck Millipore).

    Techniques: In Vitro, Activity Assay, High Performance Liquid Chromatography, Concentration Assay, Mass Spectrometry