seppak c18 cartridges Search Results


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  • 93
    Waters Corporation seppak c 18 cartridges
    Seppak C 18 Cartridges, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 44 article reviews
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    95
    Waters Corporation seppak c18 cartridge
    Seppak C18 Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 95/100, based on 314 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Millipore c18 seppak cartridge
    C18 Seppak Cartridge, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Millipore seppak c18 disposable cartridges
    Seppak C18 Disposable Cartridges, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Waters Corporation c18 seppak classic cartridge
    C18 Seppak Classic Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Waters Corporation c18 seppak spe cartridges
    C18 Seppak Spe Cartridges, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Waters Corporation 6cc seppak c18 cartridge
    6cc Seppak C18 Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 87/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation seppak c18 purification cartridges
    Seppak C18 Purification Cartridges, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Waters Corporation seppak plus c18 cartridge column
    Seppak Plus C18 Cartridge Column, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 84/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies bond elut seppak c18 cartridge
    Bond Elut Seppak C18 Cartridge, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 95/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Waters Corporation seppak c18 reverse phase cartridge
    Seppak C18 Reverse Phase Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Waters Corporation c18 light seppak cartridge
    C18 Light Seppak Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    Millipore c18 reversed phase seppak cartridges
    ( A ) Inhibition of eotaxin-induced 111 In-eosinophil accumulation by antieotaxin antibody. Purified eotaxin (1.5 pmol) was coinjected with antieotaxin IgG (▪, 1–30 μl), control IgG (♦, 30 μl) or without IgG (•, upper dotted line ) into the skin of naive guinea pigs that had received an i.v. injection of 111 In-eosinophils. Results are presented as a mean±SEM of 111 In-eosinophils/skin site after 4 h ( n = 4–6 bioassay animals). Responses to the intradermal vehicle (HBSS/0.25% BSA) alone are shown by the lower dotted line. Antieotaxin (□, 30 μl) or control IgG (⋄, 30 μl) added to the HBSS/BSA did not induce significant 111 In-eosinophil accumulation. ( B ) Inhibition of 111 In-eosinophil chemoattractant activity in 6 h BAL fluid by antieotaxin antibody. BAL fluid obtained 6 h after exposure of naive or sensitized guinea pigs to aerosolized saline or ovalbumin was concentrated by <t>C18</t> <t>SepPak</t> chromatography (see Materials and Methods) and coinjected with 50 μl control IgG ( open bars ) or antieotaxin IgG ( solid bars ) into the skin of naive guinea pigs which had received an i.v. injection of 111 In-eosinophils. Responses to HBSS/BSA + control IgG or antieotaxin antibodies were also determined. Results are presented as a mean±SEM ( n = 4 bioassay animals) and significant differences between control IgG and antieotaxin IgG treated samples are indicated as * P
    C18 Reversed Phase Seppak Cartridges, supplied by Millipore, used in various techniques. Bioz Stars score: 81/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Waters Corporation reversed phase seppak c18 cartridges
    ( A ) Inhibition of eotaxin-induced 111 In-eosinophil accumulation by antieotaxin antibody. Purified eotaxin (1.5 pmol) was coinjected with antieotaxin IgG (▪, 1–30 μl), control IgG (♦, 30 μl) or without IgG (•, upper dotted line ) into the skin of naive guinea pigs that had received an i.v. injection of 111 In-eosinophils. Results are presented as a mean±SEM of 111 In-eosinophils/skin site after 4 h ( n = 4–6 bioassay animals). Responses to the intradermal vehicle (HBSS/0.25% BSA) alone are shown by the lower dotted line. Antieotaxin (□, 30 μl) or control IgG (⋄, 30 μl) added to the HBSS/BSA did not induce significant 111 In-eosinophil accumulation. ( B ) Inhibition of 111 In-eosinophil chemoattractant activity in 6 h BAL fluid by antieotaxin antibody. BAL fluid obtained 6 h after exposure of naive or sensitized guinea pigs to aerosolized saline or ovalbumin was concentrated by <t>C18</t> <t>SepPak</t> chromatography (see Materials and Methods) and coinjected with 50 μl control IgG ( open bars ) or antieotaxin IgG ( solid bars ) into the skin of naive guinea pigs which had received an i.v. injection of 111 In-eosinophils. Responses to HBSS/BSA + control IgG or antieotaxin antibodies were also determined. Results are presented as a mean±SEM ( n = 4 bioassay animals) and significant differences between control IgG and antieotaxin IgG treated samples are indicated as * P
    Reversed Phase Seppak C18 Cartridges, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Waters Corporation seppak vac 1cc c18 cartridges
    ( A ) Inhibition of eotaxin-induced 111 In-eosinophil accumulation by antieotaxin antibody. Purified eotaxin (1.5 pmol) was coinjected with antieotaxin IgG (▪, 1–30 μl), control IgG (♦, 30 μl) or without IgG (•, upper dotted line ) into the skin of naive guinea pigs that had received an i.v. injection of 111 In-eosinophils. Results are presented as a mean±SEM of 111 In-eosinophils/skin site after 4 h ( n = 4–6 bioassay animals). Responses to the intradermal vehicle (HBSS/0.25% BSA) alone are shown by the lower dotted line. Antieotaxin (□, 30 μl) or control IgG (⋄, 30 μl) added to the HBSS/BSA did not induce significant 111 In-eosinophil accumulation. ( B ) Inhibition of 111 In-eosinophil chemoattractant activity in 6 h BAL fluid by antieotaxin antibody. BAL fluid obtained 6 h after exposure of naive or sensitized guinea pigs to aerosolized saline or ovalbumin was concentrated by <t>C18</t> <t>SepPak</t> chromatography (see Materials and Methods) and coinjected with 50 μl control IgG ( open bars ) or antieotaxin IgG ( solid bars ) into the skin of naive guinea pigs which had received an i.v. injection of 111 In-eosinophils. Responses to HBSS/BSA + control IgG or antieotaxin antibodies were also determined. Results are presented as a mean±SEM ( n = 4 bioassay animals) and significant differences between control IgG and antieotaxin IgG treated samples are indicated as * P
    Seppak Vac 1cc C18 Cartridges, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 87/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Agilent technologies c18 seppak cartridges
    ( A ) Inhibition of eotaxin-induced 111 In-eosinophil accumulation by antieotaxin antibody. Purified eotaxin (1.5 pmol) was coinjected with antieotaxin IgG (▪, 1–30 μl), control IgG (♦, 30 μl) or without IgG (•, upper dotted line ) into the skin of naive guinea pigs that had received an i.v. injection of 111 In-eosinophils. Results are presented as a mean±SEM of 111 In-eosinophils/skin site after 4 h ( n = 4–6 bioassay animals). Responses to the intradermal vehicle (HBSS/0.25% BSA) alone are shown by the lower dotted line. Antieotaxin (□, 30 μl) or control IgG (⋄, 30 μl) added to the HBSS/BSA did not induce significant 111 In-eosinophil accumulation. ( B ) Inhibition of 111 In-eosinophil chemoattractant activity in 6 h BAL fluid by antieotaxin antibody. BAL fluid obtained 6 h after exposure of naive or sensitized guinea pigs to aerosolized saline or ovalbumin was concentrated by <t>C18</t> <t>SepPak</t> chromatography (see Materials and Methods) and coinjected with 50 μl control IgG ( open bars ) or antieotaxin IgG ( solid bars ) into the skin of naive guinea pigs which had received an i.v. injection of 111 In-eosinophils. Responses to HBSS/BSA + control IgG or antieotaxin antibodies were also determined. Results are presented as a mean±SEM ( n = 4 bioassay animals) and significant differences between control IgG and antieotaxin IgG treated samples are indicated as * P
    C18 Seppak Cartridges, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 7 article reviews
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    c18 seppak cartridges - by Bioz Stars, 2020-04
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    85
    Avantor seppak c18 cartridge
    ( A ) Inhibition of eotaxin-induced 111 In-eosinophil accumulation by antieotaxin antibody. Purified eotaxin (1.5 pmol) was coinjected with antieotaxin IgG (▪, 1–30 μl), control IgG (♦, 30 μl) or without IgG (•, upper dotted line ) into the skin of naive guinea pigs that had received an i.v. injection of 111 In-eosinophils. Results are presented as a mean±SEM of 111 In-eosinophils/skin site after 4 h ( n = 4–6 bioassay animals). Responses to the intradermal vehicle (HBSS/0.25% BSA) alone are shown by the lower dotted line. Antieotaxin (□, 30 μl) or control IgG (⋄, 30 μl) added to the HBSS/BSA did not induce significant 111 In-eosinophil accumulation. ( B ) Inhibition of 111 In-eosinophil chemoattractant activity in 6 h BAL fluid by antieotaxin antibody. BAL fluid obtained 6 h after exposure of naive or sensitized guinea pigs to aerosolized saline or ovalbumin was concentrated by <t>C18</t> <t>SepPak</t> chromatography (see Materials and Methods) and coinjected with 50 μl control IgG ( open bars ) or antieotaxin IgG ( solid bars ) into the skin of naive guinea pigs which had received an i.v. injection of 111 In-eosinophils. Responses to HBSS/BSA + control IgG or antieotaxin antibodies were also determined. Results are presented as a mean±SEM ( n = 4 bioassay animals) and significant differences between control IgG and antieotaxin IgG treated samples are indicated as * P
    Seppak C18 Cartridge, supplied by Avantor, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Hichrom Limited c18 seppak cartridges
    ( A ) Inhibition of eotaxin-induced 111 In-eosinophil accumulation by antieotaxin antibody. Purified eotaxin (1.5 pmol) was coinjected with antieotaxin IgG (▪, 1–30 μl), control IgG (♦, 30 μl) or without IgG (•, upper dotted line ) into the skin of naive guinea pigs that had received an i.v. injection of 111 In-eosinophils. Results are presented as a mean±SEM of 111 In-eosinophils/skin site after 4 h ( n = 4–6 bioassay animals). Responses to the intradermal vehicle (HBSS/0.25% BSA) alone are shown by the lower dotted line. Antieotaxin (□, 30 μl) or control IgG (⋄, 30 μl) added to the HBSS/BSA did not induce significant 111 In-eosinophil accumulation. ( B ) Inhibition of 111 In-eosinophil chemoattractant activity in 6 h BAL fluid by antieotaxin antibody. BAL fluid obtained 6 h after exposure of naive or sensitized guinea pigs to aerosolized saline or ovalbumin was concentrated by <t>C18</t> <t>SepPak</t> chromatography (see Materials and Methods) and coinjected with 50 μl control IgG ( open bars ) or antieotaxin IgG ( solid bars ) into the skin of naive guinea pigs which had received an i.v. injection of 111 In-eosinophils. Responses to HBSS/BSA + control IgG or antieotaxin antibodies were also determined. Results are presented as a mean±SEM ( n = 4 bioassay animals) and significant differences between control IgG and antieotaxin IgG treated samples are indicated as * P
    C18 Seppak Cartridges, supplied by Hichrom Limited, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 3 article reviews
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    92
    Waters Corporation seppak c18 solid phase extraction cartridge
    ( A ) Inhibition of eotaxin-induced 111 In-eosinophil accumulation by antieotaxin antibody. Purified eotaxin (1.5 pmol) was coinjected with antieotaxin IgG (▪, 1–30 μl), control IgG (♦, 30 μl) or without IgG (•, upper dotted line ) into the skin of naive guinea pigs that had received an i.v. injection of 111 In-eosinophils. Results are presented as a mean±SEM of 111 In-eosinophils/skin site after 4 h ( n = 4–6 bioassay animals). Responses to the intradermal vehicle (HBSS/0.25% BSA) alone are shown by the lower dotted line. Antieotaxin (□, 30 μl) or control IgG (⋄, 30 μl) added to the HBSS/BSA did not induce significant 111 In-eosinophil accumulation. ( B ) Inhibition of 111 In-eosinophil chemoattractant activity in 6 h BAL fluid by antieotaxin antibody. BAL fluid obtained 6 h after exposure of naive or sensitized guinea pigs to aerosolized saline or ovalbumin was concentrated by <t>C18</t> <t>SepPak</t> chromatography (see Materials and Methods) and coinjected with 50 μl control IgG ( open bars ) or antieotaxin IgG ( solid bars ) into the skin of naive guinea pigs which had received an i.v. injection of 111 In-eosinophils. Responses to HBSS/BSA + control IgG or antieotaxin antibodies were also determined. Results are presented as a mean±SEM ( n = 4 bioassay animals) and significant differences between control IgG and antieotaxin IgG treated samples are indicated as * P
    Seppak C18 Solid Phase Extraction Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 10 article reviews
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    86
    Hichrom Limited high cap seppak c18 cartridge grace alltech
    ( A ) Inhibition of eotaxin-induced 111 In-eosinophil accumulation by antieotaxin antibody. Purified eotaxin (1.5 pmol) was coinjected with antieotaxin IgG (▪, 1–30 μl), control IgG (♦, 30 μl) or without IgG (•, upper dotted line ) into the skin of naive guinea pigs that had received an i.v. injection of 111 In-eosinophils. Results are presented as a mean±SEM of 111 In-eosinophils/skin site after 4 h ( n = 4–6 bioassay animals). Responses to the intradermal vehicle (HBSS/0.25% BSA) alone are shown by the lower dotted line. Antieotaxin (□, 30 μl) or control IgG (⋄, 30 μl) added to the HBSS/BSA did not induce significant 111 In-eosinophil accumulation. ( B ) Inhibition of 111 In-eosinophil chemoattractant activity in 6 h BAL fluid by antieotaxin antibody. BAL fluid obtained 6 h after exposure of naive or sensitized guinea pigs to aerosolized saline or ovalbumin was concentrated by <t>C18</t> <t>SepPak</t> chromatography (see Materials and Methods) and coinjected with 50 μl control IgG ( open bars ) or antieotaxin IgG ( solid bars ) into the skin of naive guinea pigs which had received an i.v. injection of 111 In-eosinophils. Responses to HBSS/BSA + control IgG or antieotaxin antibodies were also determined. Results are presented as a mean±SEM ( n = 4 bioassay animals) and significant differences between control IgG and antieotaxin IgG treated samples are indicated as * P
    High Cap Seppak C18 Cartridge Grace Alltech, supplied by Hichrom Limited, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    high cap seppak c18 cartridge grace alltech - by Bioz Stars, 2020-04
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    85
    Waters Corporation 360 mg c18 seppak cartridge
    ( A ) Inhibition of eotaxin-induced 111 In-eosinophil accumulation by antieotaxin antibody. Purified eotaxin (1.5 pmol) was coinjected with antieotaxin IgG (▪, 1–30 μl), control IgG (♦, 30 μl) or without IgG (•, upper dotted line ) into the skin of naive guinea pigs that had received an i.v. injection of 111 In-eosinophils. Results are presented as a mean±SEM of 111 In-eosinophils/skin site after 4 h ( n = 4–6 bioassay animals). Responses to the intradermal vehicle (HBSS/0.25% BSA) alone are shown by the lower dotted line. Antieotaxin (□, 30 μl) or control IgG (⋄, 30 μl) added to the HBSS/BSA did not induce significant 111 In-eosinophil accumulation. ( B ) Inhibition of 111 In-eosinophil chemoattractant activity in 6 h BAL fluid by antieotaxin antibody. BAL fluid obtained 6 h after exposure of naive or sensitized guinea pigs to aerosolized saline or ovalbumin was concentrated by <t>C18</t> <t>SepPak</t> chromatography (see Materials and Methods) and coinjected with 50 μl control IgG ( open bars ) or antieotaxin IgG ( solid bars ) into the skin of naive guinea pigs which had received an i.v. injection of 111 In-eosinophils. Responses to HBSS/BSA + control IgG or antieotaxin antibodies were also determined. Results are presented as a mean±SEM ( n = 4 bioassay animals) and significant differences between control IgG and antieotaxin IgG treated samples are indicated as * P
    360 Mg C18 Seppak Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Millipore seppak c18 light cartridges
    ( A ) Inhibition of eotaxin-induced 111 In-eosinophil accumulation by antieotaxin antibody. Purified eotaxin (1.5 pmol) was coinjected with antieotaxin IgG (▪, 1–30 μl), control IgG (♦, 30 μl) or without IgG (•, upper dotted line ) into the skin of naive guinea pigs that had received an i.v. injection of 111 In-eosinophils. Results are presented as a mean±SEM of 111 In-eosinophils/skin site after 4 h ( n = 4–6 bioassay animals). Responses to the intradermal vehicle (HBSS/0.25% BSA) alone are shown by the lower dotted line. Antieotaxin (□, 30 μl) or control IgG (⋄, 30 μl) added to the HBSS/BSA did not induce significant 111 In-eosinophil accumulation. ( B ) Inhibition of 111 In-eosinophil chemoattractant activity in 6 h BAL fluid by antieotaxin antibody. BAL fluid obtained 6 h after exposure of naive or sensitized guinea pigs to aerosolized saline or ovalbumin was concentrated by <t>C18</t> <t>SepPak</t> chromatography (see Materials and Methods) and coinjected with 50 μl control IgG ( open bars ) or antieotaxin IgG ( solid bars ) into the skin of naive guinea pigs which had received an i.v. injection of 111 In-eosinophils. Responses to HBSS/BSA + control IgG or antieotaxin antibodies were also determined. Results are presented as a mean±SEM ( n = 4 bioassay animals) and significant differences between control IgG and antieotaxin IgG treated samples are indicated as * P
    Seppak C18 Light Cartridges, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Waters Corporation preactivated seppak classic c18 cartridge
    ( A ) Inhibition of eotaxin-induced 111 In-eosinophil accumulation by antieotaxin antibody. Purified eotaxin (1.5 pmol) was coinjected with antieotaxin IgG (▪, 1–30 μl), control IgG (♦, 30 μl) or without IgG (•, upper dotted line ) into the skin of naive guinea pigs that had received an i.v. injection of 111 In-eosinophils. Results are presented as a mean±SEM of 111 In-eosinophils/skin site after 4 h ( n = 4–6 bioassay animals). Responses to the intradermal vehicle (HBSS/0.25% BSA) alone are shown by the lower dotted line. Antieotaxin (□, 30 μl) or control IgG (⋄, 30 μl) added to the HBSS/BSA did not induce significant 111 In-eosinophil accumulation. ( B ) Inhibition of 111 In-eosinophil chemoattractant activity in 6 h BAL fluid by antieotaxin antibody. BAL fluid obtained 6 h after exposure of naive or sensitized guinea pigs to aerosolized saline or ovalbumin was concentrated by <t>C18</t> <t>SepPak</t> chromatography (see Materials and Methods) and coinjected with 50 μl control IgG ( open bars ) or antieotaxin IgG ( solid bars ) into the skin of naive guinea pigs which had received an i.v. injection of 111 In-eosinophils. Responses to HBSS/BSA + control IgG or antieotaxin antibodies were also determined. Results are presented as a mean±SEM ( n = 4 bioassay animals) and significant differences between control IgG and antieotaxin IgG treated samples are indicated as * P
    Preactivated Seppak Classic C18 Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 87/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ( A ) Inhibition of eotaxin-induced 111 In-eosinophil accumulation by antieotaxin antibody. Purified eotaxin (1.5 pmol) was coinjected with antieotaxin IgG (▪, 1–30 μl), control IgG (♦, 30 μl) or without IgG (•, upper dotted line ) into the skin of naive guinea pigs that had received an i.v. injection of 111 In-eosinophils. Results are presented as a mean±SEM of 111 In-eosinophils/skin site after 4 h ( n = 4–6 bioassay animals). Responses to the intradermal vehicle (HBSS/0.25% BSA) alone are shown by the lower dotted line. Antieotaxin (□, 30 μl) or control IgG (⋄, 30 μl) added to the HBSS/BSA did not induce significant 111 In-eosinophil accumulation. ( B ) Inhibition of 111 In-eosinophil chemoattractant activity in 6 h BAL fluid by antieotaxin antibody. BAL fluid obtained 6 h after exposure of naive or sensitized guinea pigs to aerosolized saline or ovalbumin was concentrated by <t>C18</t> <t>SepPak</t> chromatography (see Materials and Methods) and coinjected with 50 μl control IgG ( open bars ) or antieotaxin IgG ( solid bars ) into the skin of naive guinea pigs which had received an i.v. injection of 111 In-eosinophils. Responses to HBSS/BSA + control IgG or antieotaxin antibodies were also determined. Results are presented as a mean±SEM ( n = 4 bioassay animals) and significant differences between control IgG and antieotaxin IgG treated samples are indicated as * P
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    ( A ) Inhibition of eotaxin-induced 111 In-eosinophil accumulation by antieotaxin antibody. Purified eotaxin (1.5 pmol) was coinjected with antieotaxin IgG (▪, 1–30 μl), control IgG (♦, 30 μl) or without IgG (•, upper dotted line ) into the skin of naive guinea pigs that had received an i.v. injection of 111 In-eosinophils. Results are presented as a mean±SEM of 111 In-eosinophils/skin site after 4 h ( n = 4–6 bioassay animals). Responses to the intradermal vehicle (HBSS/0.25% BSA) alone are shown by the lower dotted line. Antieotaxin (□, 30 μl) or control IgG (⋄, 30 μl) added to the HBSS/BSA did not induce significant 111 In-eosinophil accumulation. ( B ) Inhibition of 111 In-eosinophil chemoattractant activity in 6 h BAL fluid by antieotaxin antibody. BAL fluid obtained 6 h after exposure of naive or sensitized guinea pigs to aerosolized saline or ovalbumin was concentrated by <t>C18</t> <t>SepPak</t> chromatography (see Materials and Methods) and coinjected with 50 μl control IgG ( open bars ) or antieotaxin IgG ( solid bars ) into the skin of naive guinea pigs which had received an i.v. injection of 111 In-eosinophils. Responses to HBSS/BSA + control IgG or antieotaxin antibodies were also determined. Results are presented as a mean±SEM ( n = 4 bioassay animals) and significant differences between control IgG and antieotaxin IgG treated samples are indicated as * P
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    ( A ) Inhibition of eotaxin-induced 111 In-eosinophil accumulation by antieotaxin antibody. Purified eotaxin (1.5 pmol) was coinjected with antieotaxin IgG (▪, 1–30 μl), control IgG (♦, 30 μl) or without IgG (•, upper dotted line ) into the skin of naive guinea pigs that had received an i.v. injection of 111 In-eosinophils. Results are presented as a mean±SEM of 111 In-eosinophils/skin site after 4 h ( n = 4–6 bioassay animals). Responses to the intradermal vehicle (HBSS/0.25% BSA) alone are shown by the lower dotted line. Antieotaxin (□, 30 μl) or control IgG (⋄, 30 μl) added to the HBSS/BSA did not induce significant 111 In-eosinophil accumulation. ( B ) Inhibition of 111 In-eosinophil chemoattractant activity in 6 h BAL fluid by antieotaxin antibody. BAL fluid obtained 6 h after exposure of naive or sensitized guinea pigs to aerosolized saline or ovalbumin was concentrated by <t>C18</t> <t>SepPak</t> chromatography (see Materials and Methods) and coinjected with 50 μl control IgG ( open bars ) or antieotaxin IgG ( solid bars ) into the skin of naive guinea pigs which had received an i.v. injection of 111 In-eosinophils. Responses to HBSS/BSA + control IgG or antieotaxin antibodies were also determined. Results are presented as a mean±SEM ( n = 4 bioassay animals) and significant differences between control IgG and antieotaxin IgG treated samples are indicated as * P
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    ( A ) Inhibition of eotaxin-induced 111 In-eosinophil accumulation by antieotaxin antibody. Purified eotaxin (1.5 pmol) was coinjected with antieotaxin IgG (▪, 1–30 μl), control IgG (♦, 30 μl) or without IgG (•, upper dotted line ) into the skin of naive guinea pigs that had received an i.v. injection of 111 In-eosinophils. Results are presented as a mean±SEM of 111 In-eosinophils/skin site after 4 h ( n = 4–6 bioassay animals). Responses to the intradermal vehicle (HBSS/0.25% BSA) alone are shown by the lower dotted line. Antieotaxin (□, 30 μl) or control IgG (⋄, 30 μl) added to the HBSS/BSA did not induce significant 111 In-eosinophil accumulation. ( B ) Inhibition of 111 In-eosinophil chemoattractant activity in 6 h BAL fluid by antieotaxin antibody. BAL fluid obtained 6 h after exposure of naive or sensitized guinea pigs to aerosolized saline or ovalbumin was concentrated by <t>C18</t> <t>SepPak</t> chromatography (see Materials and Methods) and coinjected with 50 μl control IgG ( open bars ) or antieotaxin IgG ( solid bars ) into the skin of naive guinea pigs which had received an i.v. injection of 111 In-eosinophils. Responses to HBSS/BSA + control IgG or antieotaxin antibodies were also determined. Results are presented as a mean±SEM ( n = 4 bioassay animals) and significant differences between control IgG and antieotaxin IgG treated samples are indicated as * P
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    ( A ) Inhibition of eotaxin-induced 111 In-eosinophil accumulation by antieotaxin antibody. Purified eotaxin (1.5 pmol) was coinjected with antieotaxin IgG (▪, 1–30 μl), control IgG (♦, 30 μl) or without IgG (•, upper dotted line ) into the skin of naive guinea pigs that had received an i.v. injection of 111 In-eosinophils. Results are presented as a mean±SEM of 111 In-eosinophils/skin site after 4 h ( n = 4–6 bioassay animals). Responses to the intradermal vehicle (HBSS/0.25% BSA) alone are shown by the lower dotted line. Antieotaxin (□, 30 μl) or control IgG (⋄, 30 μl) added to the HBSS/BSA did not induce significant 111 In-eosinophil accumulation. ( B ) Inhibition of 111 In-eosinophil chemoattractant activity in 6 h BAL fluid by antieotaxin antibody. BAL fluid obtained 6 h after exposure of naive or sensitized guinea pigs to aerosolized saline or ovalbumin was concentrated by <t>C18</t> <t>SepPak</t> chromatography (see Materials and Methods) and coinjected with 50 μl control IgG ( open bars ) or antieotaxin IgG ( solid bars ) into the skin of naive guinea pigs which had received an i.v. injection of 111 In-eosinophils. Responses to HBSS/BSA + control IgG or antieotaxin antibodies were also determined. Results are presented as a mean±SEM ( n = 4 bioassay animals) and significant differences between control IgG and antieotaxin IgG treated samples are indicated as * P
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    ( A ) Inhibition of eotaxin-induced 111 In-eosinophil accumulation by antieotaxin antibody. Purified eotaxin (1.5 pmol) was coinjected with antieotaxin IgG (▪, 1–30 μl), control IgG (♦, 30 μl) or without IgG (•, upper dotted line ) into the skin of naive guinea pigs that had received an i.v. injection of 111 In-eosinophils. Results are presented as a mean±SEM of 111 In-eosinophils/skin site after 4 h ( n = 4–6 bioassay animals). Responses to the intradermal vehicle (HBSS/0.25% BSA) alone are shown by the lower dotted line. Antieotaxin (□, 30 μl) or control IgG (⋄, 30 μl) added to the HBSS/BSA did not induce significant 111 In-eosinophil accumulation. ( B ) Inhibition of 111 In-eosinophil chemoattractant activity in 6 h BAL fluid by antieotaxin antibody. BAL fluid obtained 6 h after exposure of naive or sensitized guinea pigs to aerosolized saline or ovalbumin was concentrated by <t>C18</t> <t>SepPak</t> chromatography (see Materials and Methods) and coinjected with 50 μl control IgG ( open bars ) or antieotaxin IgG ( solid bars ) into the skin of naive guinea pigs which had received an i.v. injection of 111 In-eosinophils. Responses to HBSS/BSA + control IgG or antieotaxin antibodies were also determined. Results are presented as a mean±SEM ( n = 4 bioassay animals) and significant differences between control IgG and antieotaxin IgG treated samples are indicated as * P
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    ( A ) Inhibition of eotaxin-induced 111 In-eosinophil accumulation by antieotaxin antibody. Purified eotaxin (1.5 pmol) was coinjected with antieotaxin IgG (▪, 1–30 μl), control IgG (♦, 30 μl) or without IgG (•, upper dotted line ) into the skin of naive guinea pigs that had received an i.v. injection of 111 In-eosinophils. Results are presented as a mean±SEM of 111 In-eosinophils/skin site after 4 h ( n = 4–6 bioassay animals). Responses to the intradermal vehicle (HBSS/0.25% BSA) alone are shown by the lower dotted line. Antieotaxin (□, 30 μl) or control IgG (⋄, 30 μl) added to the HBSS/BSA did not induce significant 111 In-eosinophil accumulation. ( B ) Inhibition of 111 In-eosinophil chemoattractant activity in 6 h BAL fluid by antieotaxin antibody. BAL fluid obtained 6 h after exposure of naive or sensitized guinea pigs to aerosolized saline or ovalbumin was concentrated by <t>C18</t> <t>SepPak</t> chromatography (see Materials and Methods) and coinjected with 50 μl control IgG ( open bars ) or antieotaxin IgG ( solid bars ) into the skin of naive guinea pigs which had received an i.v. injection of 111 In-eosinophils. Responses to HBSS/BSA + control IgG or antieotaxin antibodies were also determined. Results are presented as a mean±SEM ( n = 4 bioassay animals) and significant differences between control IgG and antieotaxin IgG treated samples are indicated as * P
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    ( A ) Inhibition of eotaxin-induced 111 In-eosinophil accumulation by antieotaxin antibody. Purified eotaxin (1.5 pmol) was coinjected with antieotaxin IgG (▪, 1–30 μl), control IgG (♦, 30 μl) or without IgG (•, upper dotted line ) into the skin of naive guinea pigs that had received an i.v. injection of 111 In-eosinophils. Results are presented as a mean±SEM of 111 In-eosinophils/skin site after 4 h ( n = 4–6 bioassay animals). Responses to the intradermal vehicle (HBSS/0.25% BSA) alone are shown by the lower dotted line. Antieotaxin (□, 30 μl) or control IgG (⋄, 30 μl) added to the HBSS/BSA did not induce significant 111 In-eosinophil accumulation. ( B ) Inhibition of 111 In-eosinophil chemoattractant activity in 6 h BAL fluid by antieotaxin antibody. BAL fluid obtained 6 h after exposure of naive or sensitized guinea pigs to aerosolized saline or ovalbumin was concentrated by <t>C18</t> <t>SepPak</t> chromatography (see Materials and Methods) and coinjected with 50 μl control IgG ( open bars ) or antieotaxin IgG ( solid bars ) into the skin of naive guinea pigs which had received an i.v. injection of 111 In-eosinophils. Responses to HBSS/BSA + control IgG or antieotaxin antibodies were also determined. Results are presented as a mean±SEM ( n = 4 bioassay animals) and significant differences between control IgG and antieotaxin IgG treated samples are indicated as * P
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    ( A ) Inhibition of eotaxin-induced 111 In-eosinophil accumulation by antieotaxin antibody. Purified eotaxin (1.5 pmol) was coinjected with antieotaxin IgG (▪, 1–30 μl), control IgG (♦, 30 μl) or without IgG (•, upper dotted line ) into the skin of naive guinea pigs that had received an i.v. injection of 111 In-eosinophils. Results are presented as a mean±SEM of 111 In-eosinophils/skin site after 4 h ( n = 4–6 bioassay animals). Responses to the intradermal vehicle (HBSS/0.25% BSA) alone are shown by the lower dotted line. Antieotaxin (□, 30 μl) or control IgG (⋄, 30 μl) added to the HBSS/BSA did not induce significant 111 In-eosinophil accumulation. ( B ) Inhibition of 111 In-eosinophil chemoattractant activity in 6 h BAL fluid by antieotaxin antibody. BAL fluid obtained 6 h after exposure of naive or sensitized guinea pigs to aerosolized saline or ovalbumin was concentrated by <t>C18</t> <t>SepPak</t> chromatography (see Materials and Methods) and coinjected with 50 μl control IgG ( open bars ) or antieotaxin IgG ( solid bars ) into the skin of naive guinea pigs which had received an i.v. injection of 111 In-eosinophils. Responses to HBSS/BSA + control IgG or antieotaxin antibodies were also determined. Results are presented as a mean±SEM ( n = 4 bioassay animals) and significant differences between control IgG and antieotaxin IgG treated samples are indicated as * P
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    Image Search Results


    ( A ) Inhibition of eotaxin-induced 111 In-eosinophil accumulation by antieotaxin antibody. Purified eotaxin (1.5 pmol) was coinjected with antieotaxin IgG (▪, 1–30 μl), control IgG (♦, 30 μl) or without IgG (•, upper dotted line ) into the skin of naive guinea pigs that had received an i.v. injection of 111 In-eosinophils. Results are presented as a mean±SEM of 111 In-eosinophils/skin site after 4 h ( n = 4–6 bioassay animals). Responses to the intradermal vehicle (HBSS/0.25% BSA) alone are shown by the lower dotted line. Antieotaxin (□, 30 μl) or control IgG (⋄, 30 μl) added to the HBSS/BSA did not induce significant 111 In-eosinophil accumulation. ( B ) Inhibition of 111 In-eosinophil chemoattractant activity in 6 h BAL fluid by antieotaxin antibody. BAL fluid obtained 6 h after exposure of naive or sensitized guinea pigs to aerosolized saline or ovalbumin was concentrated by C18 SepPak chromatography (see Materials and Methods) and coinjected with 50 μl control IgG ( open bars ) or antieotaxin IgG ( solid bars ) into the skin of naive guinea pigs which had received an i.v. injection of 111 In-eosinophils. Responses to HBSS/BSA + control IgG or antieotaxin antibodies were also determined. Results are presented as a mean±SEM ( n = 4 bioassay animals) and significant differences between control IgG and antieotaxin IgG treated samples are indicated as * P

    Journal: The Journal of Experimental Medicine

    Article Title: Kinetics of Eotaxin Generation and Its Relationship to Eosinophil Accumulation in Allergic Airways Disease: Analysis in a Guinea Pig Model In Vivo

    doi:

    Figure Lengend Snippet: ( A ) Inhibition of eotaxin-induced 111 In-eosinophil accumulation by antieotaxin antibody. Purified eotaxin (1.5 pmol) was coinjected with antieotaxin IgG (▪, 1–30 μl), control IgG (♦, 30 μl) or without IgG (•, upper dotted line ) into the skin of naive guinea pigs that had received an i.v. injection of 111 In-eosinophils. Results are presented as a mean±SEM of 111 In-eosinophils/skin site after 4 h ( n = 4–6 bioassay animals). Responses to the intradermal vehicle (HBSS/0.25% BSA) alone are shown by the lower dotted line. Antieotaxin (□, 30 μl) or control IgG (⋄, 30 μl) added to the HBSS/BSA did not induce significant 111 In-eosinophil accumulation. ( B ) Inhibition of 111 In-eosinophil chemoattractant activity in 6 h BAL fluid by antieotaxin antibody. BAL fluid obtained 6 h after exposure of naive or sensitized guinea pigs to aerosolized saline or ovalbumin was concentrated by C18 SepPak chromatography (see Materials and Methods) and coinjected with 50 μl control IgG ( open bars ) or antieotaxin IgG ( solid bars ) into the skin of naive guinea pigs which had received an i.v. injection of 111 In-eosinophils. Responses to HBSS/BSA + control IgG or antieotaxin antibodies were also determined. Results are presented as a mean±SEM ( n = 4 bioassay animals) and significant differences between control IgG and antieotaxin IgG treated samples are indicated as * P

    Article Snippet: To investigate eosinophil chemoattractant activity in BAL fluid, obtained 6 h after aerosolized saline or ovalbumin, samples were concentrated by use of C18 reversed phase SepPak cartridges, eluted with acetonitrile in 0.1% trifluoroacetic acid, lyophilized, and redissolved in HBSS/0.1% BSA ( < 0.1 ng endotoxin/mg albumin).

    Techniques: Inhibition, Purification, Injection, Activity Assay, Chromatography