sepharose cl 4b Millipore Search Results


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  • 98
    Millipore sepharose cl 4b
    SDS-PAGE analysis of the salivary mucus eluted in the V o of the <t>Sepharose</t> <t>CL-4B</t> gel filtration column. Freeze-dried material (30 μg) of the V o prepared in reducing gel loading buffer was separated by 10% SDS-PAGE and stained with Coomassie Brilliant Blue G-250 (a) and PAS (b). The molecular weight marker is indicated by Mw and lanes 1 to 6 represents samples from 6 donors.
    Sepharose Cl 4b, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 642 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sepharose cl 4b/product/Millipore
    Average 98 stars, based on 642 article reviews
    Price from $9.99 to $1999.99
    sepharose cl 4b - by Bioz Stars, 2020-08
    98/100 stars
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    94
    Millipore phenyl sepharose cl 4b
    SDS-PAGE analysis for homogeneity and relative molecular weight of human liver TP isolated by column chromatography. Left gel was stained by Coomassie blue and the right gel by silver staining technique. Lane A , cytosolic extract of human liver (15 μg protein); Lane B , DEAE- cellulose fraction (15 μg protein); Lane C , Phenyl <t>Sepharose</t> <t>CL</t> 4B fraction (20 μg protein); Lane D . Hydroxyapatite first peak fraction (10 μg protein); Lane E , Hydroxyapatite second peak fraction (10 μg protein); Lane M , Molecular weight markers.
    Phenyl Sepharose Cl 4b, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phenyl sepharose cl 4b/product/Millipore
    Average 94 stars, based on 63 article reviews
    Price from $9.99 to $1999.99
    phenyl sepharose cl 4b - by Bioz Stars, 2020-08
    94/100 stars
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    94
    Millipore stock solution sepharose cl 4b
    SDS-PAGE analysis for homogeneity and relative molecular weight of human liver TP isolated by column chromatography. Left gel was stained by Coomassie blue and the right gel by silver staining technique. Lane A , cytosolic extract of human liver (15 μg protein); Lane B , DEAE- cellulose fraction (15 μg protein); Lane C , Phenyl <t>Sepharose</t> <t>CL</t> 4B fraction (20 μg protein); Lane D . Hydroxyapatite first peak fraction (10 μg protein); Lane E , Hydroxyapatite second peak fraction (10 μg protein); Lane M , Molecular weight markers.
    Stock Solution Sepharose Cl 4b, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stock solution sepharose cl 4b/product/Millipore
    Average 94 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    stock solution sepharose cl 4b - by Bioz Stars, 2020-08
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    96
    Millipore cyanogen bromide activated sepharose cl 4b
    SDS-PAGE analysis for homogeneity and relative molecular weight of human liver TP isolated by column chromatography. Left gel was stained by Coomassie blue and the right gel by silver staining technique. Lane A , cytosolic extract of human liver (15 μg protein); Lane B , DEAE- cellulose fraction (15 μg protein); Lane C , Phenyl <t>Sepharose</t> <t>CL</t> 4B fraction (20 μg protein); Lane D . Hydroxyapatite first peak fraction (10 μg protein); Lane E , Hydroxyapatite second peak fraction (10 μg protein); Lane M , Molecular weight markers.
    Cyanogen Bromide Activated Sepharose Cl 4b, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyanogen bromide activated sepharose cl 4b/product/Millipore
    Average 96 stars, based on 6 article reviews
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    cyanogen bromide activated sepharose cl 4b - by Bioz Stars, 2020-08
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    93
    Millipore protein a sepharose cl 4b
    SDS-PAGE analysis for homogeneity and relative molecular weight of human liver TP isolated by column chromatography. Left gel was stained by Coomassie blue and the right gel by silver staining technique. Lane A , cytosolic extract of human liver (15 μg protein); Lane B , DEAE- cellulose fraction (15 μg protein); Lane C , Phenyl <t>Sepharose</t> <t>CL</t> 4B fraction (20 μg protein); Lane D . Hydroxyapatite first peak fraction (10 μg protein); Lane E , Hydroxyapatite second peak fraction (10 μg protein); Lane M , Molecular weight markers.
    Protein A Sepharose Cl 4b, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 606 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 606 article reviews
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    84
    Millipore tris 2 carboxyethyl phosphine immobilized on agarose cl 4b
    SDS-PAGE analysis for homogeneity and relative molecular weight of human liver TP isolated by column chromatography. Left gel was stained by Coomassie blue and the right gel by silver staining technique. Lane A , cytosolic extract of human liver (15 μg protein); Lane B , DEAE- cellulose fraction (15 μg protein); Lane C , Phenyl <t>Sepharose</t> <t>CL</t> 4B fraction (20 μg protein); Lane D . Hydroxyapatite first peak fraction (10 μg protein); Lane E , Hydroxyapatite second peak fraction (10 μg protein); Lane M , Molecular weight markers.
    Tris 2 Carboxyethyl Phosphine Immobilized On Agarose Cl 4b, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    SDS-PAGE analysis of the salivary mucus eluted in the V o of the Sepharose CL-4B gel filtration column. Freeze-dried material (30 μg) of the V o prepared in reducing gel loading buffer was separated by 10% SDS-PAGE and stained with Coomassie Brilliant Blue G-250 (a) and PAS (b). The molecular weight marker is indicated by Mw and lanes 1 to 6 represents samples from 6 donors.

    Journal: Virology Journal

    Article Title: The role of crude human saliva and purified salivary MUC5B and MUC7 mucins in the inhibition of Human Immunodeficiency Virus type 1 in an inhibition assay

    doi: 10.1186/1743-422X-3-99

    Figure Lengend Snippet: SDS-PAGE analysis of the salivary mucus eluted in the V o of the Sepharose CL-4B gel filtration column. Freeze-dried material (30 μg) of the V o prepared in reducing gel loading buffer was separated by 10% SDS-PAGE and stained with Coomassie Brilliant Blue G-250 (a) and PAS (b). The molecular weight marker is indicated by Mw and lanes 1 to 6 represents samples from 6 donors.

    Article Snippet: Sepharose CL-4B and reagent solvents such as guanidinium chloride (GuHCl), phenylmethylsulfonylfluoride (PMSF), caesium chloride (CsCl), ethylenediaminetetra-acetic acid disodium salt (Na2 -EDTA), N-ethylmaleimide (NEM), and 3-((3-cholamidopropyl)-dimethyl-ammonio)-1-propane-sulfonate (CHAPS) were from Sigma (UK).

    Techniques: SDS Page, Filtration, Staining, Molecular Weight, Marker

    SDS-PAGE analysis of the salivary mucus eluted in the V i of the Sepharose CL-4B gel filtration column. Freeze-dried material (30 μg) of the V i prepared in reducing gel loading buffer was separated by 10% SDS-PAGE and stained with Coomassie Brilliant Blue G-250 (a) and PAS (b). The molecular weight marker is indicated by Mw and lanes 1 to 6 represents samples from 6 donors.

    Journal: Virology Journal

    Article Title: The role of crude human saliva and purified salivary MUC5B and MUC7 mucins in the inhibition of Human Immunodeficiency Virus type 1 in an inhibition assay

    doi: 10.1186/1743-422X-3-99

    Figure Lengend Snippet: SDS-PAGE analysis of the salivary mucus eluted in the V i of the Sepharose CL-4B gel filtration column. Freeze-dried material (30 μg) of the V i prepared in reducing gel loading buffer was separated by 10% SDS-PAGE and stained with Coomassie Brilliant Blue G-250 (a) and PAS (b). The molecular weight marker is indicated by Mw and lanes 1 to 6 represents samples from 6 donors.

    Article Snippet: Sepharose CL-4B and reagent solvents such as guanidinium chloride (GuHCl), phenylmethylsulfonylfluoride (PMSF), caesium chloride (CsCl), ethylenediaminetetra-acetic acid disodium salt (Na2 -EDTA), N-ethylmaleimide (NEM), and 3-((3-cholamidopropyl)-dimethyl-ammonio)-1-propane-sulfonate (CHAPS) were from Sigma (UK).

    Techniques: SDS Page, Filtration, Staining, Molecular Weight, Marker

    Sepharose CL-4B gel filtration of crude saliva. An aliquot (20 ml) of soluble human crude saliva extracted in 6 M GuHCl containing 10 mM EDTA, 5 mM NEM, 1 mM PMSF and 0.1% CHAPS pH 6.5 was chromatographed and eluted with 4 M GuHCl containing 10 mM EDTA, 5 mM NEM and 0.05% CHAPS pH 6.5 at flow rate of 48 ml/h at room temperature. Fractions were analysed for carbohydrate with PAS at 555 nm (◆) and for protein A 280 (■). Materials eluted in the void volume (V o ) and included volume (V i ) were pooled separately, dialysed against three changes of distilled water for overnight at 4°C and freeze-dried.

    Journal: Virology Journal

    Article Title: The role of crude human saliva and purified salivary MUC5B and MUC7 mucins in the inhibition of Human Immunodeficiency Virus type 1 in an inhibition assay

    doi: 10.1186/1743-422X-3-99

    Figure Lengend Snippet: Sepharose CL-4B gel filtration of crude saliva. An aliquot (20 ml) of soluble human crude saliva extracted in 6 M GuHCl containing 10 mM EDTA, 5 mM NEM, 1 mM PMSF and 0.1% CHAPS pH 6.5 was chromatographed and eluted with 4 M GuHCl containing 10 mM EDTA, 5 mM NEM and 0.05% CHAPS pH 6.5 at flow rate of 48 ml/h at room temperature. Fractions were analysed for carbohydrate with PAS at 555 nm (◆) and for protein A 280 (■). Materials eluted in the void volume (V o ) and included volume (V i ) were pooled separately, dialysed against three changes of distilled water for overnight at 4°C and freeze-dried.

    Article Snippet: Sepharose CL-4B and reagent solvents such as guanidinium chloride (GuHCl), phenylmethylsulfonylfluoride (PMSF), caesium chloride (CsCl), ethylenediaminetetra-acetic acid disodium salt (Na2 -EDTA), N-ethylmaleimide (NEM), and 3-((3-cholamidopropyl)-dimethyl-ammonio)-1-propane-sulfonate (CHAPS) were from Sigma (UK).

    Techniques: Filtration, Flow Cytometry

    SDS-PAGE analysis for homogeneity and relative molecular weight of human liver TP isolated by column chromatography. Left gel was stained by Coomassie blue and the right gel by silver staining technique. Lane A , cytosolic extract of human liver (15 μg protein); Lane B , DEAE- cellulose fraction (15 μg protein); Lane C , Phenyl Sepharose CL 4B fraction (20 μg protein); Lane D . Hydroxyapatite first peak fraction (10 μg protein); Lane E , Hydroxyapatite second peak fraction (10 μg protein); Lane M , Molecular weight markers.

    Journal: PLoS ONE

    Article Title: Distinct substrate specificity and physicochemical characterization of native human hepatic thymidine phosphorylase

    doi: 10.1371/journal.pone.0202826

    Figure Lengend Snippet: SDS-PAGE analysis for homogeneity and relative molecular weight of human liver TP isolated by column chromatography. Left gel was stained by Coomassie blue and the right gel by silver staining technique. Lane A , cytosolic extract of human liver (15 μg protein); Lane B , DEAE- cellulose fraction (15 μg protein); Lane C , Phenyl Sepharose CL 4B fraction (20 μg protein); Lane D . Hydroxyapatite first peak fraction (10 μg protein); Lane E , Hydroxyapatite second peak fraction (10 μg protein); Lane M , Molecular weight markers.

    Article Snippet: [2-14 C]thymidine (56 Ci/mol), [2-14 C]uridine (56 Ci/mol), [2-14 C]2ʹ-deoxyuridine (56 Ci/mol), [2-14 C]5-fluorouridine (56 Ci/mol), [2-14 C]5-fluoro-2´-deoxyuridine (56 Ci/mol), and [6-3 H]5´-deoxy-5-fluorouridine (7 Ci/mmol) were purchased from Moravek Biochemicals Inc., Brea, CA; Macherey Nagel Polygram Silica Gel G/UV254 thin layer chromatography plates from Fisher Scientific, Pittsburgh, PA.; DEAE-cellulose anion exchanger (fast flow; fibrous form), Phenyl Sepharose CL 4B, Sephadex G-150, Sephadex G-100, Polybuffer exchanger 94, Polybuffer 74, and gel filtration calibration (LMW and HMW) kit from Sigma Chemical Co., St. Louis, MO; Bio-Gel HTP hydroxyapatite, silver stain plus kit, Bio-Rad protein assay kit, Coomassie Brilliant Blue R-250, alkaline Phosphatase, 5-bromo-4-chloro-3-indoylphosphate p -toluidine salt, and p -nitro blue tetrazolium chloride from Bio-Rad Laboratories, Hercules, CA; Diaflo ultrafiltration membrane (YM30, 43 mm diameter), and Centricon-30 concentrator from Amicon Inc., Beverly, MA; Spectra/Por 2 membrane dialysis tubing (12–14,000 molecular weight cut off, 105 mm flat width) from Spectrum Medical Industries Inc., Houston, TX; Ultrafree-MC filters of 10,000 NMWL regenerated cellulose membrane from Millipore Corp., Bedford, MA; TaqStart Antibody from Clontech Laboratories Inc., Palo Alto, CA; Immobilon-P transfer membrane, and anhydrous hydrazine from Pierce Chemical Company Inc., Rockford, IL; Goat Anti-Rabbit IgG-AP (conjugated with alkaline phosphatase) from Southern Biotechnology Associates Inc., Birmingham, AL; TWEEN 20 (polyoxyethylene (20) sorbitan monolaurate), and Cyanogen bromide (CNBr) from Aldrich Chemical Company Inc., Milwaukee, WI.

    Techniques: SDS Page, Molecular Weight, Isolation, Column Chromatography, Staining, Silver Staining

    Purification of human TP by column chromatography. ( A ) DEAE-cellulose ion exchange chromatography, ( B ) Phenyl Sepharose CL-4B chromatography, ( C ) Bio-Gel HTP Hydroxyapatite chromatography.

    Journal: PLoS ONE

    Article Title: Distinct substrate specificity and physicochemical characterization of native human hepatic thymidine phosphorylase

    doi: 10.1371/journal.pone.0202826

    Figure Lengend Snippet: Purification of human TP by column chromatography. ( A ) DEAE-cellulose ion exchange chromatography, ( B ) Phenyl Sepharose CL-4B chromatography, ( C ) Bio-Gel HTP Hydroxyapatite chromatography.

    Article Snippet: [2-14 C]thymidine (56 Ci/mol), [2-14 C]uridine (56 Ci/mol), [2-14 C]2ʹ-deoxyuridine (56 Ci/mol), [2-14 C]5-fluorouridine (56 Ci/mol), [2-14 C]5-fluoro-2´-deoxyuridine (56 Ci/mol), and [6-3 H]5´-deoxy-5-fluorouridine (7 Ci/mmol) were purchased from Moravek Biochemicals Inc., Brea, CA; Macherey Nagel Polygram Silica Gel G/UV254 thin layer chromatography plates from Fisher Scientific, Pittsburgh, PA.; DEAE-cellulose anion exchanger (fast flow; fibrous form), Phenyl Sepharose CL 4B, Sephadex G-150, Sephadex G-100, Polybuffer exchanger 94, Polybuffer 74, and gel filtration calibration (LMW and HMW) kit from Sigma Chemical Co., St. Louis, MO; Bio-Gel HTP hydroxyapatite, silver stain plus kit, Bio-Rad protein assay kit, Coomassie Brilliant Blue R-250, alkaline Phosphatase, 5-bromo-4-chloro-3-indoylphosphate p -toluidine salt, and p -nitro blue tetrazolium chloride from Bio-Rad Laboratories, Hercules, CA; Diaflo ultrafiltration membrane (YM30, 43 mm diameter), and Centricon-30 concentrator from Amicon Inc., Beverly, MA; Spectra/Por 2 membrane dialysis tubing (12–14,000 molecular weight cut off, 105 mm flat width) from Spectrum Medical Industries Inc., Houston, TX; Ultrafree-MC filters of 10,000 NMWL regenerated cellulose membrane from Millipore Corp., Bedford, MA; TaqStart Antibody from Clontech Laboratories Inc., Palo Alto, CA; Immobilon-P transfer membrane, and anhydrous hydrazine from Pierce Chemical Company Inc., Rockford, IL; Goat Anti-Rabbit IgG-AP (conjugated with alkaline phosphatase) from Southern Biotechnology Associates Inc., Birmingham, AL; TWEEN 20 (polyoxyethylene (20) sorbitan monolaurate), and Cyanogen bromide (CNBr) from Aldrich Chemical Company Inc., Milwaukee, WI.

    Techniques: Purification, Column Chromatography, Ion Exchange Chromatography, Chromatography