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  • 94
    Millipore selective agar plates
    Selective Agar Plates, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 2 article reviews
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    selective agar plates - by Bioz Stars, 2020-04
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    99
    Thermo Fisher select agar powder
    Select Agar Powder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore enterococcus selective agar
    Enterococcus Selective Agar, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore campylobacter selective agar
    Cultivability of Helicobacter and <t>Campylobacter</t> spp. in water on CBA at 15°C (top) and 37°C (bottom) in the absence of light over time. Error bars correspond to standard deviations of results from three replicate experiments.
    Campylobacter Selective Agar, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Millipore b cereus selective agar
    Cultivability of Helicobacter and <t>Campylobacter</t> spp. in water on CBA at 15°C (top) and 37°C (bottom) in the absence of light over time. Error bars correspond to standard deviations of results from three replicate experiments.
    B Cereus Selective Agar, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 89 stars, based on 1 article reviews
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    99
    Thermo Fisher campylobacter selective agar
    Comparison of flagellins isolated from <t>Campylobacter</t> spp. and MBP-FlaA isolated from E. coli ) and cross-absorbed with whole cells of E. coli ); C, LL1 antiserum against MBP-FlaA at a dilution of 1:20,000; D, ferret antiserum from an animal infected with strain 81-176 at a dilution of 1:500; E, human antiserum from a human volunteer infected with 81-176 at a dilution of 1:5,000. Lane 1, VC167 T2 flagellin; lane 2, 81-176 flagellin; lane 3, MBP-FlaA. VC167 T2 flagellin migrates at an apparent M r ), and 81-176 flagellin migrates at an apparent M r ).
    Campylobacter Selective Agar, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher yersinia selective agar
    Identification of a temperature-responsive bistable switch. ( A ) The temperature-responsive <t>Yersinia</t> virulence regulator RovA is autoregulated through positive and negative feedback loops. At 25°C RovA is active and binds cooperatively to a high-affinity site upstream of P2 and activates rovA and invA transcription. When the RovA amount has reached a certain threshold, RovA binds to a low affinity site downstream of P1 to prevent uncontrolled rovA induction. An upshift to 37°C induces a reversible conformational change in RovA that leads to a strong reduction of its DNA-binding capacity and renders this regulator susceptible to proteolysis by the Lon protease. rovA transcription is further regulated by the nutrient-responsive repressor RovM. ( B ) Y . pseudotuberculosis wild-type carrying a P rovA - egfp LVA fusion was grown at different temperatures and analysed by fluorescence microscopy, and ( C ), flow cytometry (one representative replicate; 10 5 cells). ( D ) The percentage of P rovA - egfp LVA -expressing wild-type cells quantified by flow cytometry (mean ± SEM; n = 3 for each temperature; 10 5 cells per replicate), eGFP LVA -positive cells (ON) are shown in green. The response of P rovA - egfp LVA to temperature corresponds to the average RovA level as determined by western blot. Relative RovA amounts were quantified and normalised to the highest temperature for which a homogenous RovA ON population was observed (mean ± SEM; n = 3 for each temperature). ( E ), Live cell imaging of Y . pseudotuberculosis expressing P rovA - egfp LVA at 32°C. Time series of individual bacteria starting from the OFF state demonstrates switching to the ON and back to the OFF state; representative overlays of eGFP LVA and bright field images at different time points are shown (also see S 1 Video ).
    Yersinia Selective Agar, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher clostridium difficile selective agar
    Identification of a temperature-responsive bistable switch. ( A ) The temperature-responsive <t>Yersinia</t> virulence regulator RovA is autoregulated through positive and negative feedback loops. At 25°C RovA is active and binds cooperatively to a high-affinity site upstream of P2 and activates rovA and invA transcription. When the RovA amount has reached a certain threshold, RovA binds to a low affinity site downstream of P1 to prevent uncontrolled rovA induction. An upshift to 37°C induces a reversible conformational change in RovA that leads to a strong reduction of its DNA-binding capacity and renders this regulator susceptible to proteolysis by the Lon protease. rovA transcription is further regulated by the nutrient-responsive repressor RovM. ( B ) Y . pseudotuberculosis wild-type carrying a P rovA - egfp LVA fusion was grown at different temperatures and analysed by fluorescence microscopy, and ( C ), flow cytometry (one representative replicate; 10 5 cells). ( D ) The percentage of P rovA - egfp LVA -expressing wild-type cells quantified by flow cytometry (mean ± SEM; n = 3 for each temperature; 10 5 cells per replicate), eGFP LVA -positive cells (ON) are shown in green. The response of P rovA - egfp LVA to temperature corresponds to the average RovA level as determined by western blot. Relative RovA amounts were quantified and normalised to the highest temperature for which a homogenous RovA ON population was observed (mean ± SEM; n = 3 for each temperature). ( E ), Live cell imaging of Y . pseudotuberculosis expressing P rovA - egfp LVA at 32°C. Time series of individual bacteria starting from the OFF state demonstrates switching to the ON and back to the OFF state; representative overlays of eGFP LVA and bright field images at different time points are shown (also see S 1 Video ).
    Clostridium Difficile Selective Agar, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore palcam listera selective agar
    Identification of a temperature-responsive bistable switch. ( A ) The temperature-responsive <t>Yersinia</t> virulence regulator RovA is autoregulated through positive and negative feedback loops. At 25°C RovA is active and binds cooperatively to a high-affinity site upstream of P2 and activates rovA and invA transcription. When the RovA amount has reached a certain threshold, RovA binds to a low affinity site downstream of P1 to prevent uncontrolled rovA induction. An upshift to 37°C induces a reversible conformational change in RovA that leads to a strong reduction of its DNA-binding capacity and renders this regulator susceptible to proteolysis by the Lon protease. rovA transcription is further regulated by the nutrient-responsive repressor RovM. ( B ) Y . pseudotuberculosis wild-type carrying a P rovA - egfp LVA fusion was grown at different temperatures and analysed by fluorescence microscopy, and ( C ), flow cytometry (one representative replicate; 10 5 cells). ( D ) The percentage of P rovA - egfp LVA -expressing wild-type cells quantified by flow cytometry (mean ± SEM; n = 3 for each temperature; 10 5 cells per replicate), eGFP LVA -positive cells (ON) are shown in green. The response of P rovA - egfp LVA to temperature corresponds to the average RovA level as determined by western blot. Relative RovA amounts were quantified and normalised to the highest temperature for which a homogenous RovA ON population was observed (mean ± SEM; n = 3 for each temperature). ( E ), Live cell imaging of Y . pseudotuberculosis expressing P rovA - egfp LVA at 32°C. Time series of individual bacteria starting from the OFF state demonstrates switching to the ON and back to the OFF state; representative overlays of eGFP LVA and bright field images at different time points are shown (also see S 1 Video ).
    Palcam Listera Selective Agar, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher yersinia selective agar base
    Specificity of PCR assay for Y. ruckeri (specific amplification of the 575-bp fragment from the total DNA of Y. ruckeri , other <t>Yersinia</t> species, and other genera). Lane 1, Y. ruckeri CECT 4319 (positive control); lane 2, no template (negative control); lane 3, 1-kb plus DNA ladder (Amersham); lanes 4 to 7, Y. ruckeri CECT 956, 93/03, 94/30, and A-1261, respectively; lanes 8 to 13, Yersinia enterocolitica CECT 4315, Yersinia kristensenii CECT 4318, Yersinia intermedia CECT 4317, Aeromonas salmonicida CECT 894, Vibrio vulnificus CECT 529, and Hafnei alvei CECT 158.
    Yersinia Selective Agar Base, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher legionella mwy selective agar
    Specificity of PCR assay for Y. ruckeri (specific amplification of the 575-bp fragment from the total DNA of Y. ruckeri , other <t>Yersinia</t> species, and other genera). Lane 1, Y. ruckeri CECT 4319 (positive control); lane 2, no template (negative control); lane 3, 1-kb plus DNA ladder (Amersham); lanes 4 to 7, Y. ruckeri CECT 956, 93/03, 94/30, and A-1261, respectively; lanes 8 to 13, Yersinia enterocolitica CECT 4315, Yersinia kristensenii CECT 4318, Yersinia intermedia CECT 4317, Aeromonas salmonicida CECT 894, Vibrio vulnificus CECT 529, and Hafnei alvei CECT 158.
    Legionella Mwy Selective Agar, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher campylobacter selective butzler agar
    Design and outcome of the animal experiment. A. Time scale of the experiment. B. Reduction of C. jejuni in the chickens. Mean values of <t>Campylobacter</t> counts (CFU/g feces) and standard deviations are shown.
    Campylobacter Selective Butzler Agar, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher legionella bmpa selective agar
    Design and outcome of the animal experiment. A. Time scale of the experiment. B. Reduction of C. jejuni in the chickens. Mean values of <t>Campylobacter</t> counts (CFU/g feces) and standard deviations are shown.
    Legionella Bmpa Selective Agar, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore streptococcus
    Design and outcome of the animal experiment. A. Time scale of the experiment. B. Reduction of C. jejuni in the chickens. Mean values of <t>Campylobacter</t> counts (CFU/g feces) and standard deviations are shown.
    Streptococcus, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher listeria selective agar
    Design and outcome of the animal experiment. A. Time scale of the experiment. B. Reduction of C. jejuni in the chickens. Mean values of <t>Campylobacter</t> counts (CFU/g feces) and standard deviations are shown.
    Listeria Selective Agar, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher legionella gvpc selective agar
    Design and outcome of the animal experiment. A. Time scale of the experiment. B. Reduction of C. jejuni in the chickens. Mean values of <t>Campylobacter</t> counts (CFU/g feces) and standard deviations are shown.
    Legionella Gvpc Selective Agar, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher karmali selective agar
    Design and outcome of the animal experiment. A. Time scale of the experiment. B. Reduction of C. jejuni in the chickens. Mean values of <t>Campylobacter</t> counts (CFU/g feces) and standard deviations are shown.
    Karmali Selective Agar, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher yersinia selective agar medium
    Design and outcome of the animal experiment. A. Time scale of the experiment. B. Reduction of C. jejuni in the chickens. Mean values of <t>Campylobacter</t> counts (CFU/g feces) and standard deviations are shown.
    Yersinia Selective Agar Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher streptococcal selective agar
    Design and outcome of the animal experiment. A. Time scale of the experiment. B. Reduction of C. jejuni in the chickens. Mean values of <t>Campylobacter</t> counts (CFU/g feces) and standard deviations are shown.
    Streptococcal Selective Agar, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Becton Dickinson 7h11 selective agar
    Microbiological outcome after 2 (A) and 4 (B) weeks of treatment with rifamycin-containing regimens. Mice were infected with 4.56 log 10 CFUs of M. ulcerans into hind footpads. After 6 weeks of incubation, treatment was initiated (D0). At this time point, the CFU mean (±SD) equaled 5.77 (±0.60). Groups of mice ( n = 5) were sacrificed at week 2 and week 4, and footpads were dissected, minced, and plated on <t>7H11</t> agar for colony counting and CFU analysis. There was a dose-dependent reduction in CFU with elevating rifamycin doses. No ceiling effect was observed for RIF. At week 4, all rifapentine-containing regimens were culture negative, as was RIF40CLR100. Numbers after drugs indicate doses in mg/kg. RIF, rifampin; STR, streptomycin; CLR, clarithromycin; RPT, rifapentine.
    7h11 Selective Agar, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Becton Dickinson campylobacter selective agar
    Microbiological outcome after 2 (A) and 4 (B) weeks of treatment with rifamycin-containing regimens. Mice were infected with 4.56 log 10 CFUs of M. ulcerans into hind footpads. After 6 weeks of incubation, treatment was initiated (D0). At this time point, the CFU mean (±SD) equaled 5.77 (±0.60). Groups of mice ( n = 5) were sacrificed at week 2 and week 4, and footpads were dissected, minced, and plated on <t>7H11</t> agar for colony counting and CFU analysis. There was a dose-dependent reduction in CFU with elevating rifamycin doses. No ceiling effect was observed for RIF. At week 4, all rifapentine-containing regimens were culture negative, as was RIF40CLR100. Numbers after drugs indicate doses in mg/kg. RIF, rifampin; STR, streptomycin; CLR, clarithromycin; RPT, rifapentine.
    Campylobacter Selective Agar, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 1 article reviews
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    87
    Merck KGaA cereus selective agar
    Microbiological outcome after 2 (A) and 4 (B) weeks of treatment with rifamycin-containing regimens. Mice were infected with 4.56 log 10 CFUs of M. ulcerans into hind footpads. After 6 weeks of incubation, treatment was initiated (D0). At this time point, the CFU mean (±SD) equaled 5.77 (±0.60). Groups of mice ( n = 5) were sacrificed at week 2 and week 4, and footpads were dissected, minced, and plated on <t>7H11</t> agar for colony counting and CFU analysis. There was a dose-dependent reduction in CFU with elevating rifamycin doses. No ceiling effect was observed for RIF. At week 4, all rifapentine-containing regimens were culture negative, as was RIF40CLR100. Numbers after drugs indicate doses in mg/kg. RIF, rifampin; STR, streptomycin; CLR, clarithromycin; RPT, rifapentine.
    Cereus Selective Agar, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Becton Dickinson lactobacillus selection agar
    Microbiological outcome after 2 (A) and 4 (B) weeks of treatment with rifamycin-containing regimens. Mice were infected with 4.56 log 10 CFUs of M. ulcerans into hind footpads. After 6 weeks of incubation, treatment was initiated (D0). At this time point, the CFU mean (±SD) equaled 5.77 (±0.60). Groups of mice ( n = 5) were sacrificed at week 2 and week 4, and footpads were dissected, minced, and plated on <t>7H11</t> agar for colony counting and CFU analysis. There was a dose-dependent reduction in CFU with elevating rifamycin doses. No ceiling effect was observed for RIF. At week 4, all rifapentine-containing regimens were culture negative, as was RIF40CLR100. Numbers after drugs indicate doses in mg/kg. RIF, rifampin; STR, streptomycin; CLR, clarithromycin; RPT, rifapentine.
    Lactobacillus Selection Agar, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    HiMedia Laboratories listeria selective agar
    Microbiological outcome after 2 (A) and 4 (B) weeks of treatment with rifamycin-containing regimens. Mice were infected with 4.56 log 10 CFUs of M. ulcerans into hind footpads. After 6 weeks of incubation, treatment was initiated (D0). At this time point, the CFU mean (±SD) equaled 5.77 (±0.60). Groups of mice ( n = 5) were sacrificed at week 2 and week 4, and footpads were dissected, minced, and plated on <t>7H11</t> agar for colony counting and CFU analysis. There was a dose-dependent reduction in CFU with elevating rifamycin doses. No ceiling effect was observed for RIF. At week 4, all rifapentine-containing regimens were culture negative, as was RIF40CLR100. Numbers after drugs indicate doses in mg/kg. RIF, rifampin; STR, streptomycin; CLR, clarithromycin; RPT, rifapentine.
    Listeria Selective Agar, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    listeria selective agar - by Bioz Stars, 2020-04
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    85
    Becton Dickinson selective chrom agar
    Microbiological outcome after 2 (A) and 4 (B) weeks of treatment with rifamycin-containing regimens. Mice were infected with 4.56 log 10 CFUs of M. ulcerans into hind footpads. After 6 weeks of incubation, treatment was initiated (D0). At this time point, the CFU mean (±SD) equaled 5.77 (±0.60). Groups of mice ( n = 5) were sacrificed at week 2 and week 4, and footpads were dissected, minced, and plated on <t>7H11</t> agar for colony counting and CFU analysis. There was a dose-dependent reduction in CFU with elevating rifamycin doses. No ceiling effect was observed for RIF. At week 4, all rifapentine-containing regimens were culture negative, as was RIF40CLR100. Numbers after drugs indicate doses in mg/kg. RIF, rifampin; STR, streptomycin; CLR, clarithromycin; RPT, rifapentine.
    Selective Chrom Agar, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    DRG International selective chromogenic agar
    Microbiological outcome after 2 (A) and 4 (B) weeks of treatment with rifamycin-containing regimens. Mice were infected with 4.56 log 10 CFUs of M. ulcerans into hind footpads. After 6 weeks of incubation, treatment was initiated (D0). At this time point, the CFU mean (±SD) equaled 5.77 (±0.60). Groups of mice ( n = 5) were sacrificed at week 2 and week 4, and footpads were dissected, minced, and plated on <t>7H11</t> agar for colony counting and CFU analysis. There was a dose-dependent reduction in CFU with elevating rifamycin doses. No ceiling effect was observed for RIF. At week 4, all rifapentine-containing regimens were culture negative, as was RIF40CLR100. Numbers after drugs indicate doses in mg/kg. RIF, rifampin; STR, streptomycin; CLR, clarithromycin; RPT, rifapentine.
    Selective Chromogenic Agar, supplied by DRG International, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    bioMerieux 7h11 selective agar
    Microbiological outcome after 2 (A) and 4 (B) weeks of treatment with rifamycin-containing regimens. Mice were infected with 4.56 log 10 CFUs of M. ulcerans into hind footpads. After 6 weeks of incubation, treatment was initiated (D0). At this time point, the CFU mean (±SD) equaled 5.77 (±0.60). Groups of mice ( n = 5) were sacrificed at week 2 and week 4, and footpads were dissected, minced, and plated on <t>7H11</t> agar for colony counting and CFU analysis. There was a dose-dependent reduction in CFU with elevating rifamycin doses. No ceiling effect was observed for RIF. At week 4, all rifapentine-containing regimens were culture negative, as was RIF40CLR100. Numbers after drugs indicate doses in mg/kg. RIF, rifampin; STR, streptomycin; CLR, clarithromycin; RPT, rifapentine.
    7h11 Selective Agar, supplied by bioMerieux, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 3 article reviews
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    Difco 7h11 selective agar
    Microbiological outcome after 2 (A) and 4 (B) weeks of treatment with rifamycin-containing regimens. Mice were infected with 4.56 log 10 CFUs of M. ulcerans into hind footpads. After 6 weeks of incubation, treatment was initiated (D0). At this time point, the CFU mean (±SD) equaled 5.77 (±0.60). Groups of mice ( n = 5) were sacrificed at week 2 and week 4, and footpads were dissected, minced, and plated on <t>7H11</t> agar for colony counting and CFU analysis. There was a dose-dependent reduction in CFU with elevating rifamycin doses. No ceiling effect was observed for RIF. At week 4, all rifapentine-containing regimens were culture negative, as was RIF40CLR100. Numbers after drugs indicate doses in mg/kg. RIF, rifampin; STR, streptomycin; CLR, clarithromycin; RPT, rifapentine.
    7h11 Selective Agar, supplied by Difco, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco campylobacter selective agar
    Microbiological outcome after 2 (A) and 4 (B) weeks of treatment with rifamycin-containing regimens. Mice were infected with 4.56 log 10 CFUs of M. ulcerans into hind footpads. After 6 weeks of incubation, treatment was initiated (D0). At this time point, the CFU mean (±SD) equaled 5.77 (±0.60). Groups of mice ( n = 5) were sacrificed at week 2 and week 4, and footpads were dissected, minced, and plated on <t>7H11</t> agar for colony counting and CFU analysis. There was a dose-dependent reduction in CFU with elevating rifamycin doses. No ceiling effect was observed for RIF. At week 4, all rifapentine-containing regimens were culture negative, as was RIF40CLR100. Numbers after drugs indicate doses in mg/kg. RIF, rifampin; STR, streptomycin; CLR, clarithromycin; RPT, rifapentine.
    Campylobacter Selective Agar, supplied by Difco, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    campylobacter selective agar - by Bioz Stars, 2020-04
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    88
    HiMedia Laboratories klebsiella selective agar
    Microbiological outcome after 2 (A) and 4 (B) weeks of treatment with rifamycin-containing regimens. Mice were infected with 4.56 log 10 CFUs of M. ulcerans into hind footpads. After 6 weeks of incubation, treatment was initiated (D0). At this time point, the CFU mean (±SD) equaled 5.77 (±0.60). Groups of mice ( n = 5) were sacrificed at week 2 and week 4, and footpads were dissected, minced, and plated on <t>7H11</t> agar for colony counting and CFU analysis. There was a dose-dependent reduction in CFU with elevating rifamycin doses. No ceiling effect was observed for RIF. At week 4, all rifapentine-containing regimens were culture negative, as was RIF40CLR100. Numbers after drugs indicate doses in mg/kg. RIF, rifampin; STR, streptomycin; CLR, clarithromycin; RPT, rifapentine.
    Klebsiella Selective Agar, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 88/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Cultivability of Helicobacter and Campylobacter spp. in water on CBA at 15°C (top) and 37°C (bottom) in the absence of light over time. Error bars correspond to standard deviations of results from three replicate experiments.

    Journal: Applied and Environmental Microbiology

    Article Title: Survival of Gastric and Enterohepatic Helicobacter spp. in Water: Implications for Transmission ▿ spp. in Water: Implications for Transmission ▿ †

    doi: 10.1128/AEM.02241-07

    Figure Lengend Snippet: Cultivability of Helicobacter and Campylobacter spp. in water on CBA at 15°C (top) and 37°C (bottom) in the absence of light over time. Error bars correspond to standard deviations of results from three replicate experiments.

    Article Snippet: Plates were incubated at 37°C in a CO2 incubator (HERAcell 150; Thermo Electron Corporation, Waltham, MA) set to 10% CO2 and 5% O2 , and bacteria were streaked onto fresh plates every 2 or 3 days; the only difference in terms of culture maintenance between H. pylori and the other Helicobacter and Campylobacter species tested was that the latter were also grown on Campylobacter selective agar (CA; Sigma) supplemented with 5% (vol/vol) defibrinated horse blood.

    Techniques: Crocin Bleaching Assay

    Transmission electron microscopy of Campylobacter jejuni in the colon of an infected ferret. (A) Electron micrograph showing attachment of 3 bacteria (arrows) to the epithelial cell surface. Notice the loss of microvilli (mv) at the site of attachment.

    Journal: Comparative Medicine

    Article Title: Immune Response to and Histopathology of Campylobacter jejuni Infection in Ferrets (Mustela putorius furo)

    doi:

    Figure Lengend Snippet: Transmission electron microscopy of Campylobacter jejuni in the colon of an infected ferret. (A) Electron micrograph showing attachment of 3 bacteria (arrows) to the epithelial cell surface. Notice the loss of microvilli (mv) at the site of attachment.

    Article Snippet: Cardiac blood mononuclear cells collected from controls ( n = 4) and animals euthanized on days 6 and 9 ( n = 6) were used to determine Campylobacter -specific IgA antibody-secreting cells (ASC) by ELISpot using a modification of a procedure published previously., After thawing and washing, viability of mononuclear cells was determined using Guava ViaCount Reagent (Guava Technologies, Hayward, CA).

    Techniques: Transmission Assay, Electron Microscopy, Infection

    Distribution of synonymous and non-synonymous mutations across the Campylobacter porA allele in relation to the protein structure predicted by Zhang et al. (2000) . (a) Plot demonstrating the average for each codon for all pairwise comparisons for indels, synonymous and non-synonymous mutations calculated from 196 porA nucleotide alleles by SNAP.pl ( Korber, 2000 ; Ota Nei, 1994 ). Synonymous mutations are indicated by the blue line and non-synonymous mutations by the pink line. x -axis annotation indicates codon number and related predicted protein structure. (b) Schematic representation of the Campylobacter MOMP indicating putative intra-membrane β -barrels and surface-exposed loops.

    Journal: Microbiology

    Article Title: Genetic diversity and stability of the porA allele as a genetic marker in human Campylobacter infection

    doi: 10.1099/mic.0.031047-0

    Figure Lengend Snippet: Distribution of synonymous and non-synonymous mutations across the Campylobacter porA allele in relation to the protein structure predicted by Zhang et al. (2000) . (a) Plot demonstrating the average for each codon for all pairwise comparisons for indels, synonymous and non-synonymous mutations calculated from 196 porA nucleotide alleles by SNAP.pl ( Korber, 2000 ; Ota Nei, 1994 ). Synonymous mutations are indicated by the blue line and non-synonymous mutations by the pink line. x -axis annotation indicates codon number and related predicted protein structure. (b) Schematic representation of the Campylobacter MOMP indicating putative intra-membrane β -barrels and surface-exposed loops.

    Article Snippet: Isolates were stored at −70 °C in Tryptone Soy Broth plus glycerol (10 %) and cell-free DNA extracts were produced from a suspension of Campylobacter cells (cultured on charcoal cefoperazone deoxycholate agar plates) in molecular biology grade water (125 μl, Sigma Aldrich).

    Techniques:

    Alignment of 31 deduced MOMP sequences chosen to represent the diversity observed among 180 peptides identified in human Campylobacter isolates. Protein variant number is indicated to the left and amino acid length to the right of each sequence. Gaps are indicated by -. Bold lines below sequence blocks indicate regions predicted to form β -barrels, and regions encoding putative external loops are labelled L4 to L8, as described by Zhang et al. (2000) .

    Journal: Microbiology

    Article Title: Genetic diversity and stability of the porA allele as a genetic marker in human Campylobacter infection

    doi: 10.1099/mic.0.031047-0

    Figure Lengend Snippet: Alignment of 31 deduced MOMP sequences chosen to represent the diversity observed among 180 peptides identified in human Campylobacter isolates. Protein variant number is indicated to the left and amino acid length to the right of each sequence. Gaps are indicated by -. Bold lines below sequence blocks indicate regions predicted to form β -barrels, and regions encoding putative external loops are labelled L4 to L8, as described by Zhang et al. (2000) .

    Article Snippet: Isolates were stored at −70 °C in Tryptone Soy Broth plus glycerol (10 %) and cell-free DNA extracts were produced from a suspension of Campylobacter cells (cultured on charcoal cefoperazone deoxycholate agar plates) in molecular biology grade water (125 μl, Sigma Aldrich).

    Techniques: Variant Assay, Sequencing

    Comparison of flagellins isolated from Campylobacter spp. and MBP-FlaA isolated from E. coli ) and cross-absorbed with whole cells of E. coli ); C, LL1 antiserum against MBP-FlaA at a dilution of 1:20,000; D, ferret antiserum from an animal infected with strain 81-176 at a dilution of 1:500; E, human antiserum from a human volunteer infected with 81-176 at a dilution of 1:5,000. Lane 1, VC167 T2 flagellin; lane 2, 81-176 flagellin; lane 3, MBP-FlaA. VC167 T2 flagellin migrates at an apparent M r ), and 81-176 flagellin migrates at an apparent M r ).

    Journal: Infection and Immunity

    Article Title: Evaluation of a Truncated Recombinant Flagellin Subunit Vaccine against Campylobacter jejuni

    doi:

    Figure Lengend Snippet: Comparison of flagellins isolated from Campylobacter spp. and MBP-FlaA isolated from E. coli ) and cross-absorbed with whole cells of E. coli ); C, LL1 antiserum against MBP-FlaA at a dilution of 1:20,000; D, ferret antiserum from an animal infected with strain 81-176 at a dilution of 1:500; E, human antiserum from a human volunteer infected with 81-176 at a dilution of 1:5,000. Lane 1, VC167 T2 flagellin; lane 2, 81-176 flagellin; lane 3, MBP-FlaA. VC167 T2 flagellin migrates at an apparent M r ), and 81-176 flagellin migrates at an apparent M r ).

    Article Snippet: 5% suspension in PBS) onto a campylobacter-selective agar (CVA; Remel, Lenexa, Kans.).

    Techniques: Isolation, Infection

    Microbial enumeration after Calcium Oxide exposure in samples from Bayou Cumbest. As can be seen, this figure displays the inactivation and survival trends of eight microbial species upon exposure to Calcium oxide. Heterotrophic , Total Coliforms , Fecal coliforms , Campylobacter , Vibrio , Enterococcus ,. E. coli and Salmonella/Shigella were inactivated at the lowest concentration (0.25%) of Calcium oxide. No species showed resistance to the effects of Calcium oxide.

    Journal: Biomedical sciences instrumentation

    Article Title: CALCIUM OXIDE REMEDIATION OF ANTHROPOGENIC CONTAMINATION OF WATER AT THE GBNERR IN MISSISSIPPI

    doi:

    Figure Lengend Snippet: Microbial enumeration after Calcium Oxide exposure in samples from Bayou Cumbest. As can be seen, this figure displays the inactivation and survival trends of eight microbial species upon exposure to Calcium oxide. Heterotrophic , Total Coliforms , Fecal coliforms , Campylobacter , Vibrio , Enterococcus ,. E. coli and Salmonella/Shigella were inactivated at the lowest concentration (0.25%) of Calcium oxide. No species showed resistance to the effects of Calcium oxide.

    Article Snippet: Campylobacter agar: Oxoid Blood-Free Campylobacter agar (Oxoid LTD., Basingstoke, Hampshire, England).

    Techniques: Concentration Assay

    Microbial enumeration after Calcium oxide exposure in samples from Bayou Point of Pines. As can be seen, this figure displays the inactivation and survival trends of eight microbial species upon exposure to Calcium oxide. Heterotrophic , Vibrio , E. coli , Salmonella/Shigella , Total coliforms , Fecal coliform , Campylobacter and Enterococcus were totally inactivated at the lowest Calcium oxide concentration of 0.25%.

    Journal: Biomedical sciences instrumentation

    Article Title: CALCIUM OXIDE REMEDIATION OF ANTHROPOGENIC CONTAMINATION OF WATER AT THE GBNERR IN MISSISSIPPI

    doi:

    Figure Lengend Snippet: Microbial enumeration after Calcium oxide exposure in samples from Bayou Point of Pines. As can be seen, this figure displays the inactivation and survival trends of eight microbial species upon exposure to Calcium oxide. Heterotrophic , Vibrio , E. coli , Salmonella/Shigella , Total coliforms , Fecal coliform , Campylobacter and Enterococcus were totally inactivated at the lowest Calcium oxide concentration of 0.25%.

    Article Snippet: Campylobacter agar: Oxoid Blood-Free Campylobacter agar (Oxoid LTD., Basingstoke, Hampshire, England).

    Techniques: Concentration Assay

    Fig. 1a . Number of laboratory-confirmed Campylobacter cases and diarrhea cases per quarter-year by patient-care setting, Santa Rosa, Guatemala 2008–2012.

    Journal: Journal of epidemiology and global health

    Article Title: Burden of laboratory-confirmed Campylobacter infections in Guatemala 2008–2012: Results from a facility-based surveillance system

    doi: 10.1016/j.jegh.2013.10.001

    Figure Lengend Snippet: Fig. 1a . Number of laboratory-confirmed Campylobacter cases and diarrhea cases per quarter-year by patient-care setting, Santa Rosa, Guatemala 2008–2012.

    Article Snippet: Samples were streaked by direct plating onto Campylobacter selective agar base, Karmali (Oxoid, Basingstoke, UK) and incubated at 42 °C for 48 h under microaerophilic conditions provided by the CampyGen™ Generating System (Oxoid, Basingstoke, UK).

    Techniques:

    Campylobacter jejuni levels are reduced by phage predation post-treatment. Independently housed Ross 308 broilers were given 7 log 10 CFU C. jejuni by oral gavage at 20 days-old. At 24 days-old birds were orally administered a mixture of two phage at 7 log 10 PFU each or a placebo of carrier alone. Five chickens from each group were sacrificed on each day from 25 to 29 days-old, from which C. jejuni were enumerated from the intestinal contents of the ceca (A) , ileum (D) , and colon (E) . Titers of phage CP20 (B) and CP30A (C) were determined from cecal content. Filled squares indicate means. Error bars indicate standard deviation. Asterix indicate statistical significance: ∗ , p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001.

    Journal: Frontiers in Microbiology

    Article Title: Phage Biocontrol of Campylobacter jejuni in Chickens Does Not Produce Collateral Effects on the Gut Microbiota

    doi: 10.3389/fmicb.2019.00476

    Figure Lengend Snippet: Campylobacter jejuni levels are reduced by phage predation post-treatment. Independently housed Ross 308 broilers were given 7 log 10 CFU C. jejuni by oral gavage at 20 days-old. At 24 days-old birds were orally administered a mixture of two phage at 7 log 10 PFU each or a placebo of carrier alone. Five chickens from each group were sacrificed on each day from 25 to 29 days-old, from which C. jejuni were enumerated from the intestinal contents of the ceca (A) , ileum (D) , and colon (E) . Titers of phage CP20 (B) and CP30A (C) were determined from cecal content. Filled squares indicate means. Error bars indicate standard deviation. Asterix indicate statistical significance: ∗ , p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001.

    Article Snippet: C. jejuni HPC5 for the inoculation of broiler chickens and campylobacter for the production of bacterial lawns were cultured on horse blood agar (Blood agar base No 2; Oxoid) with addition of plus 5% (v/v) defibrinated horse blood, (TCS, Buckingham, United Kingdom) under microaerobic conditions (5% O2 , 5% H2 , 10% CO2 , 80% N2 , produced by the evacuation and replacement technique) at 42°C for 24 h.

    Techniques: Standard Deviation

    Identification of a temperature-responsive bistable switch. ( A ) The temperature-responsive Yersinia virulence regulator RovA is autoregulated through positive and negative feedback loops. At 25°C RovA is active and binds cooperatively to a high-affinity site upstream of P2 and activates rovA and invA transcription. When the RovA amount has reached a certain threshold, RovA binds to a low affinity site downstream of P1 to prevent uncontrolled rovA induction. An upshift to 37°C induces a reversible conformational change in RovA that leads to a strong reduction of its DNA-binding capacity and renders this regulator susceptible to proteolysis by the Lon protease. rovA transcription is further regulated by the nutrient-responsive repressor RovM. ( B ) Y . pseudotuberculosis wild-type carrying a P rovA - egfp LVA fusion was grown at different temperatures and analysed by fluorescence microscopy, and ( C ), flow cytometry (one representative replicate; 10 5 cells). ( D ) The percentage of P rovA - egfp LVA -expressing wild-type cells quantified by flow cytometry (mean ± SEM; n = 3 for each temperature; 10 5 cells per replicate), eGFP LVA -positive cells (ON) are shown in green. The response of P rovA - egfp LVA to temperature corresponds to the average RovA level as determined by western blot. Relative RovA amounts were quantified and normalised to the highest temperature for which a homogenous RovA ON population was observed (mean ± SEM; n = 3 for each temperature). ( E ), Live cell imaging of Y . pseudotuberculosis expressing P rovA - egfp LVA at 32°C. Time series of individual bacteria starting from the OFF state demonstrates switching to the ON and back to the OFF state; representative overlays of eGFP LVA and bright field images at different time points are shown (also see S 1 Video ).

    Journal: PLoS Pathogens

    Article Title: A Precise Temperature-Responsive Bistable Switch Controlling Yersinia Virulence

    doi: 10.1371/journal.ppat.1006091

    Figure Lengend Snippet: Identification of a temperature-responsive bistable switch. ( A ) The temperature-responsive Yersinia virulence regulator RovA is autoregulated through positive and negative feedback loops. At 25°C RovA is active and binds cooperatively to a high-affinity site upstream of P2 and activates rovA and invA transcription. When the RovA amount has reached a certain threshold, RovA binds to a low affinity site downstream of P1 to prevent uncontrolled rovA induction. An upshift to 37°C induces a reversible conformational change in RovA that leads to a strong reduction of its DNA-binding capacity and renders this regulator susceptible to proteolysis by the Lon protease. rovA transcription is further regulated by the nutrient-responsive repressor RovM. ( B ) Y . pseudotuberculosis wild-type carrying a P rovA - egfp LVA fusion was grown at different temperatures and analysed by fluorescence microscopy, and ( C ), flow cytometry (one representative replicate; 10 5 cells). ( D ) The percentage of P rovA - egfp LVA -expressing wild-type cells quantified by flow cytometry (mean ± SEM; n = 3 for each temperature; 10 5 cells per replicate), eGFP LVA -positive cells (ON) are shown in green. The response of P rovA - egfp LVA to temperature corresponds to the average RovA level as determined by western blot. Relative RovA amounts were quantified and normalised to the highest temperature for which a homogenous RovA ON population was observed (mean ± SEM; n = 3 for each temperature). ( E ), Live cell imaging of Y . pseudotuberculosis expressing P rovA - egfp LVA at 32°C. Time series of individual bacteria starting from the OFF state demonstrates switching to the ON and back to the OFF state; representative overlays of eGFP LVA and bright field images at different time points are shown (also see S 1 Video ).

    Article Snippet: E . coli strain S17-1λpir harbouring the plasmids were used for conjugation and the resulting transconjugants were identified by plating on Yersinia selective agar (Oxoid) supplemented with chloramphenicol.

    Techniques: Binding Assay, Fluorescence, Microscopy, Flow Cytometry, Cytometry, Expressing, Western Blot, Live Cell Imaging

    Analysis of Y. pestis and Y. pseudotuberculosis replication in macrophages by microscopy. C57BL/6 macrophages were infected with KIM10 + /GFP (a to f) or IP2790c/GFP (g to l) at an MOI of 5. The infected cells were incubated for 4 h (a to c and g to i) or 25 h (d to f and j to l) and then fixed, and bacteria were labeled with a rabbit anti- Yersinia antibody (red). One hour before fixation, IPTG was added to the cells to induce GFP expression in viable bacteria (green). The samples were visualized by phase (left panels) or epifluorescence (middle and right panels) microscopy. Representative images were captured using a digital camera. Macrophages containing large numbers of intracellular bacteria displayed an enlarged and vacuolated morphology under phase-contrast microscopy (arrowheads).

    Journal: Infection and Immunity

    Article Title: The Ability To Replicate in Macrophages Is Conserved between Yersinia pestis and Yersinia pseudotuberculosis

    doi: 10.1128/IAI.71.10.5892-5899.2003

    Figure Lengend Snippet: Analysis of Y. pestis and Y. pseudotuberculosis replication in macrophages by microscopy. C57BL/6 macrophages were infected with KIM10 + /GFP (a to f) or IP2790c/GFP (g to l) at an MOI of 5. The infected cells were incubated for 4 h (a to c and g to i) or 25 h (d to f and j to l) and then fixed, and bacteria were labeled with a rabbit anti- Yersinia antibody (red). One hour before fixation, IPTG was added to the cells to induce GFP expression in viable bacteria (green). The samples were visualized by phase (left panels) or epifluorescence (middle and right panels) microscopy. Representative images were captured using a digital camera. Macrophages containing large numbers of intracellular bacteria displayed an enlarged and vacuolated morphology under phase-contrast microscopy (arrowheads).

    Article Snippet: Transconjugants were selected on M63 minimal agar plates ( ) or Yersinia selective agar plates (Oxoid) containing kanamycin.

    Techniques: Microscopy, Infection, Incubation, Labeling, Expressing

    Temperature-dependent variations in susceptibility of Yersinia enterocolitica to antimicrobial peptides.

    Journal: Journal of Bacteriology

    Article Title: Molecular Basis of Yersinia enterocolitica Temperature-Dependent Resistance to Antimicrobial Peptides

    doi: 10.1128/JB.00308-12

    Figure Lengend Snippet: Temperature-dependent variations in susceptibility of Yersinia enterocolitica to antimicrobial peptides.

    Article Snippet: The bacterial loads recovered from the infected organs were determined by plating homogenates and serial dilutions onto Yersinia selective agar medium plates (Oxoid) to select YeO8 and YeO8-Δ pmrF and onto Km-containing LB plates to select YeO8-Δ pagP GB and YeO8-Δ pmrF -Δ pagP GB.

    Techniques:

    Specificity of PCR assay for Y. ruckeri (specific amplification of the 575-bp fragment from the total DNA of Y. ruckeri , other Yersinia species, and other genera). Lane 1, Y. ruckeri CECT 4319 (positive control); lane 2, no template (negative control); lane 3, 1-kb plus DNA ladder (Amersham); lanes 4 to 7, Y. ruckeri CECT 956, 93/03, 94/30, and A-1261, respectively; lanes 8 to 13, Yersinia enterocolitica CECT 4315, Yersinia kristensenii CECT 4318, Yersinia intermedia CECT 4317, Aeromonas salmonicida CECT 894, Vibrio vulnificus CECT 529, and Hafnei alvei CECT 158.

    Journal: Applied and Environmental Microbiology

    Article Title: Development of a PCR Assay for Detection of Yersinia ruckeri in Tissues of Inoculated and Naturally Infected Trout

    doi:

    Figure Lengend Snippet: Specificity of PCR assay for Y. ruckeri (specific amplification of the 575-bp fragment from the total DNA of Y. ruckeri , other Yersinia species, and other genera). Lane 1, Y. ruckeri CECT 4319 (positive control); lane 2, no template (negative control); lane 3, 1-kb plus DNA ladder (Amersham); lanes 4 to 7, Y. ruckeri CECT 956, 93/03, 94/30, and A-1261, respectively; lanes 8 to 13, Yersinia enterocolitica CECT 4315, Yersinia kristensenii CECT 4318, Yersinia intermedia CECT 4317, Aeromonas salmonicida CECT 894, Vibrio vulnificus CECT 529, and Hafnei alvei CECT 158.

    Article Snippet: The enrichment broth was prepared by allowing the agar of the yersinia-selective agar base (Oxoid) to sediment, and the supernatant was recovered.

    Techniques: Polymerase Chain Reaction, Amplification, Positive Control, Negative Control

    Design and outcome of the animal experiment. A. Time scale of the experiment. B. Reduction of C. jejuni in the chickens. Mean values of Campylobacter counts (CFU/g feces) and standard deviations are shown.

    Journal: PLoS ONE

    Article Title: Reduction of Campylobacter jejuni in Broiler Chicken by Successive Application of Group II and Group III Phages

    doi: 10.1371/journal.pone.0114785

    Figure Lengend Snippet: Design and outcome of the animal experiment. A. Time scale of the experiment. B. Reduction of C. jejuni in the chickens. Mean values of Campylobacter counts (CFU/g feces) and standard deviations are shown.

    Article Snippet: 100 µl aliquots of each suspension were plated on Karmali agar containing a Campylobacter selective supplement (Oxoid).

    Techniques:

    Nucleotide-binding oligomerization domain 2 (NOD2) deficiency exacerbates neutrophil infiltration–induced crypt abscesses in Campylobacter jejuni –infected mice. Cohorts of 9–18 Il10 −/− and Il10 −/−

    Journal: The Journal of Infectious Diseases

    Article Title: Nucleotide-Binding Oligomerization Domain–Containing Protein 2 Controls Host Response to Campylobacter jejuni in Il10−/− Mice

    doi: 10.1093/infdis/jiu148

    Figure Lengend Snippet: Nucleotide-binding oligomerization domain 2 (NOD2) deficiency exacerbates neutrophil infiltration–induced crypt abscesses in Campylobacter jejuni –infected mice. Cohorts of 9–18 Il10 −/− and Il10 −/−

    Article Snippet: To quantify viable C. jejuni in intestinal and extraintestinal tissues, we aseptically collected samples from the colon and MLNs and enumerated the bacteria on Remel Campylobacter -selective plates.

    Techniques: Binding Assay, Infection, Mouse Assay

    Nucleotide-binding oligomerization domain 2 (NOD2) promotes Campylobacter jejuni –induced iNos but attenuates Cxcl1 and Il1β messenger RNA (mRNA) expression in macrophages. Elicited peritoneal macrophages from Il10 −/− and

    Journal: The Journal of Infectious Diseases

    Article Title: Nucleotide-Binding Oligomerization Domain–Containing Protein 2 Controls Host Response to Campylobacter jejuni in Il10−/− Mice

    doi: 10.1093/infdis/jiu148

    Figure Lengend Snippet: Nucleotide-binding oligomerization domain 2 (NOD2) promotes Campylobacter jejuni –induced iNos but attenuates Cxcl1 and Il1β messenger RNA (mRNA) expression in macrophages. Elicited peritoneal macrophages from Il10 −/− and

    Article Snippet: To quantify viable C. jejuni in intestinal and extraintestinal tissues, we aseptically collected samples from the colon and MLNs and enumerated the bacteria on Remel Campylobacter -selective plates.

    Techniques: Binding Assay, Expressing

    Nucleotide-binding oligomerization domain 2 (NOD2) enhances nitric oxide (NO) production and promotes Campylobacter jejuni clearance in macrophages. Elicited peritoneal macrophages from Il10 −/− and Il10 −/− ; Nod2 −/−

    Journal: The Journal of Infectious Diseases

    Article Title: Nucleotide-Binding Oligomerization Domain–Containing Protein 2 Controls Host Response to Campylobacter jejuni in Il10−/− Mice

    doi: 10.1093/infdis/jiu148

    Figure Lengend Snippet: Nucleotide-binding oligomerization domain 2 (NOD2) enhances nitric oxide (NO) production and promotes Campylobacter jejuni clearance in macrophages. Elicited peritoneal macrophages from Il10 −/− and Il10 −/− ; Nod2 −/−

    Article Snippet: To quantify viable C. jejuni in intestinal and extraintestinal tissues, we aseptically collected samples from the colon and MLNs and enumerated the bacteria on Remel Campylobacter -selective plates.

    Techniques: Binding Assay

    Supplementation with nitric oxide (NO) attenuates Campylobacter jejuni– induced intestinal inflammation. Cohorts of 5–8 Il10 −/− and Il10 −/− ; Nod2 −/− mice raised in specific-pathogen-free conditions

    Journal: The Journal of Infectious Diseases

    Article Title: Nucleotide-Binding Oligomerization Domain–Containing Protein 2 Controls Host Response to Campylobacter jejuni in Il10−/− Mice

    doi: 10.1093/infdis/jiu148

    Figure Lengend Snippet: Supplementation with nitric oxide (NO) attenuates Campylobacter jejuni– induced intestinal inflammation. Cohorts of 5–8 Il10 −/− and Il10 −/− ; Nod2 −/− mice raised in specific-pathogen-free conditions

    Article Snippet: To quantify viable C. jejuni in intestinal and extraintestinal tissues, we aseptically collected samples from the colon and MLNs and enumerated the bacteria on Remel Campylobacter -selective plates.

    Techniques: Mouse Assay

    Nucleotide-binding oligomerization domain 2 (NOD2) enhances Campylobacter jejuni clearance in macrophages. Elicited peritoneal macrophages from Il10 −/− and Il10 −/− ; Nod2 −/− mice were infected with C. jejuni

    Journal: The Journal of Infectious Diseases

    Article Title: Nucleotide-Binding Oligomerization Domain–Containing Protein 2 Controls Host Response to Campylobacter jejuni in Il10−/− Mice

    doi: 10.1093/infdis/jiu148

    Figure Lengend Snippet: Nucleotide-binding oligomerization domain 2 (NOD2) enhances Campylobacter jejuni clearance in macrophages. Elicited peritoneal macrophages from Il10 −/− and Il10 −/− ; Nod2 −/− mice were infected with C. jejuni

    Article Snippet: To quantify viable C. jejuni in intestinal and extraintestinal tissues, we aseptically collected samples from the colon and MLNs and enumerated the bacteria on Remel Campylobacter -selective plates.

    Techniques: Binding Assay, Mouse Assay, Infection

    Nucleotide-binding oligomerization domain 2 (NOD2) prevents Campylobacter jejuni invasion into the colon and mesenteric lymph nodes. Cohorts of 9–18 Il10 −/− and Il10 −/− ; Nod2 −/− mice raised in specific-pathogen-free

    Journal: The Journal of Infectious Diseases

    Article Title: Nucleotide-Binding Oligomerization Domain–Containing Protein 2 Controls Host Response to Campylobacter jejuni in Il10−/− Mice

    doi: 10.1093/infdis/jiu148

    Figure Lengend Snippet: Nucleotide-binding oligomerization domain 2 (NOD2) prevents Campylobacter jejuni invasion into the colon and mesenteric lymph nodes. Cohorts of 9–18 Il10 −/− and Il10 −/− ; Nod2 −/− mice raised in specific-pathogen-free

    Article Snippet: To quantify viable C. jejuni in intestinal and extraintestinal tissues, we aseptically collected samples from the colon and MLNs and enumerated the bacteria on Remel Campylobacter -selective plates.

    Techniques: Binding Assay, Mouse Assay

    Nucleotide-binding oligomerization domain 2 (NOD2) attenuates Campylobacter jejuni –induced intestinal inflammation. Cohorts of 6–18 Nod2 −/− , Il10 −/− , and Il10 −/− ; Nod2 −/−

    Journal: The Journal of Infectious Diseases

    Article Title: Nucleotide-Binding Oligomerization Domain–Containing Protein 2 Controls Host Response to Campylobacter jejuni in Il10−/− Mice

    doi: 10.1093/infdis/jiu148

    Figure Lengend Snippet: Nucleotide-binding oligomerization domain 2 (NOD2) attenuates Campylobacter jejuni –induced intestinal inflammation. Cohorts of 6–18 Nod2 −/− , Il10 −/− , and Il10 −/− ; Nod2 −/−

    Article Snippet: To quantify viable C. jejuni in intestinal and extraintestinal tissues, we aseptically collected samples from the colon and MLNs and enumerated the bacteria on Remel Campylobacter -selective plates.

    Techniques: Binding Assay

    Microbiological outcome after 2 (A) and 4 (B) weeks of treatment with rifamycin-containing regimens. Mice were infected with 4.56 log 10 CFUs of M. ulcerans into hind footpads. After 6 weeks of incubation, treatment was initiated (D0). At this time point, the CFU mean (±SD) equaled 5.77 (±0.60). Groups of mice ( n = 5) were sacrificed at week 2 and week 4, and footpads were dissected, minced, and plated on 7H11 agar for colony counting and CFU analysis. There was a dose-dependent reduction in CFU with elevating rifamycin doses. No ceiling effect was observed for RIF. At week 4, all rifapentine-containing regimens were culture negative, as was RIF40CLR100. Numbers after drugs indicate doses in mg/kg. RIF, rifampin; STR, streptomycin; CLR, clarithromycin; RPT, rifapentine.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: High-Dose Rifamycins Enable Shorter Oral Treatment in a Murine Model of Mycobacterium ulcerans Disease

    doi: 10.1128/AAC.01478-18

    Figure Lengend Snippet: Microbiological outcome after 2 (A) and 4 (B) weeks of treatment with rifamycin-containing regimens. Mice were infected with 4.56 log 10 CFUs of M. ulcerans into hind footpads. After 6 weeks of incubation, treatment was initiated (D0). At this time point, the CFU mean (±SD) equaled 5.77 (±0.60). Groups of mice ( n = 5) were sacrificed at week 2 and week 4, and footpads were dissected, minced, and plated on 7H11 agar for colony counting and CFU analysis. There was a dose-dependent reduction in CFU with elevating rifamycin doses. No ceiling effect was observed for RIF. At week 4, all rifapentine-containing regimens were culture negative, as was RIF40CLR100. Numbers after drugs indicate doses in mg/kg. RIF, rifampin; STR, streptomycin; CLR, clarithromycin; RPT, rifapentine.

    Article Snippet: All bacteria were cultured on Middlebrook 7H11 selective agar (Becton, Dickinson, Sparks, MD) at 32°C.

    Techniques: Mouse Assay, Infection, Incubation