second strand cdna synthesis Search Results


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  • 99
    New England Biolabs nebnext ultra directional second strand cdna synthesis protocol
    Nebnext Ultra Directional Second Strand Cdna Synthesis Protocol, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher second strand cdna synthesis
    Fig. 3. Glucocorticoids induce MKP-1 gene expression. RBL-2H3 mast cells ( A ), mouse bone marrow-derived mast cells ( B ) and NIH-3T3 fibroblasts ( C ) were treated for the indicated time with dexamethasone (Dex, 0.1 µM) or solvent alone. Total <t>RNA</t> was extracted and subjected to northern blotting using <t>cDNA</t> probes for MKP-1 and GAPDH. ( D ) COS-7 cells were transiently co-transfected with an MKP-1 promoter firefly luciferase construct (-1716MKP-1luc) and a Renilla luciferase construct as an internal control, together with either wild-type glucocorticoid receptor (GRwt) or two different dimerization/transactivation-deficient GR mutant [A458T or hGR(D4X)] expression vectors. Treatment with dexamethasone (Dex, 0.1 µM) or solvent alone was performed 4 h after the transfection and the cells were harvested 48 h later for luciferase activity measurements. The results are expressed as the level of MKP-1 promoter-driven firefly luciferase expression after correcting for the transfection efficiency by Renilla luciferase measurements (relative luciferase activity), and are presented as the mean ± SD of three independent experiments.
    Second Strand Cdna Synthesis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 832 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc second strand cdna synthesis
    Fig. 3. Glucocorticoids induce MKP-1 gene expression. RBL-2H3 mast cells ( A ), mouse bone marrow-derived mast cells ( B ) and NIH-3T3 fibroblasts ( C ) were treated for the indicated time with dexamethasone (Dex, 0.1 µM) or solvent alone. Total <t>RNA</t> was extracted and subjected to northern blotting using <t>cDNA</t> probes for MKP-1 and GAPDH. ( D ) COS-7 cells were transiently co-transfected with an MKP-1 promoter firefly luciferase construct (-1716MKP-1luc) and a Renilla luciferase construct as an internal control, together with either wild-type glucocorticoid receptor (GRwt) or two different dimerization/transactivation-deficient GR mutant [A458T or hGR(D4X)] expression vectors. Treatment with dexamethasone (Dex, 0.1 µM) or solvent alone was performed 4 h after the transfection and the cells were harvested 48 h later for luciferase activity measurements. The results are expressed as the level of MKP-1 promoter-driven firefly luciferase expression after correcting for the transfection efficiency by Renilla luciferase measurements (relative luciferase activity), and are presented as the mean ± SD of three independent experiments.
    Second Strand Cdna Synthesis, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 654 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher second strand buffer
    Fig. 3. Glucocorticoids induce MKP-1 gene expression. RBL-2H3 mast cells ( A ), mouse bone marrow-derived mast cells ( B ) and NIH-3T3 fibroblasts ( C ) were treated for the indicated time with dexamethasone (Dex, 0.1 µM) or solvent alone. Total <t>RNA</t> was extracted and subjected to northern blotting using <t>cDNA</t> probes for MKP-1 and GAPDH. ( D ) COS-7 cells were transiently co-transfected with an MKP-1 promoter firefly luciferase construct (-1716MKP-1luc) and a Renilla luciferase construct as an internal control, together with either wild-type glucocorticoid receptor (GRwt) or two different dimerization/transactivation-deficient GR mutant [A458T or hGR(D4X)] expression vectors. Treatment with dexamethasone (Dex, 0.1 µM) or solvent alone was performed 4 h after the transfection and the cells were harvested 48 h later for luciferase activity measurements. The results are expressed as the level of MKP-1 promoter-driven firefly luciferase expression after correcting for the transfection efficiency by Renilla luciferase measurements (relative luciferase activity), and are presented as the mean ± SD of three independent experiments.
    Second Strand Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 506 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Beyotime second strand cdna synthesis kit
    Fig. 3. Glucocorticoids induce MKP-1 gene expression. RBL-2H3 mast cells ( A ), mouse bone marrow-derived mast cells ( B ) and NIH-3T3 fibroblasts ( C ) were treated for the indicated time with dexamethasone (Dex, 0.1 µM) or solvent alone. Total <t>RNA</t> was extracted and subjected to northern blotting using <t>cDNA</t> probes for MKP-1 and GAPDH. ( D ) COS-7 cells were transiently co-transfected with an MKP-1 promoter firefly luciferase construct (-1716MKP-1luc) and a Renilla luciferase construct as an internal control, together with either wild-type glucocorticoid receptor (GRwt) or two different dimerization/transactivation-deficient GR mutant [A458T or hGR(D4X)] expression vectors. Treatment with dexamethasone (Dex, 0.1 µM) or solvent alone was performed 4 h after the transfection and the cells were harvested 48 h later for luciferase activity measurements. The results are expressed as the level of MKP-1 promoter-driven firefly luciferase expression after correcting for the transfection efficiency by Renilla luciferase measurements (relative luciferase activity), and are presented as the mean ± SD of three independent experiments.
    Second Strand Cdna Synthesis Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 88/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rnase h mediated second strand cdna synthesis
    Fig. 3. Glucocorticoids induce MKP-1 gene expression. RBL-2H3 mast cells ( A ), mouse bone marrow-derived mast cells ( B ) and NIH-3T3 fibroblasts ( C ) were treated for the indicated time with dexamethasone (Dex, 0.1 µM) or solvent alone. Total <t>RNA</t> was extracted and subjected to northern blotting using <t>cDNA</t> probes for MKP-1 and GAPDH. ( D ) COS-7 cells were transiently co-transfected with an MKP-1 promoter firefly luciferase construct (-1716MKP-1luc) and a Renilla luciferase construct as an internal control, together with either wild-type glucocorticoid receptor (GRwt) or two different dimerization/transactivation-deficient GR mutant [A458T or hGR(D4X)] expression vectors. Treatment with dexamethasone (Dex, 0.1 µM) or solvent alone was performed 4 h after the transfection and the cells were harvested 48 h later for luciferase activity measurements. The results are expressed as the level of MKP-1 promoter-driven firefly luciferase expression after correcting for the transfection efficiency by Renilla luciferase measurements (relative luciferase activity), and are presented as the mean ± SD of three independent experiments.
    Rnase H Mediated Second Strand Cdna Synthesis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc second strand complementary dna cdna synthesis
    Fig. 3. Glucocorticoids induce MKP-1 gene expression. RBL-2H3 mast cells ( A ), mouse bone marrow-derived mast cells ( B ) and NIH-3T3 fibroblasts ( C ) were treated for the indicated time with dexamethasone (Dex, 0.1 µM) or solvent alone. Total <t>RNA</t> was extracted and subjected to northern blotting using <t>cDNA</t> probes for MKP-1 and GAPDH. ( D ) COS-7 cells were transiently co-transfected with an MKP-1 promoter firefly luciferase construct (-1716MKP-1luc) and a Renilla luciferase construct as an internal control, together with either wild-type glucocorticoid receptor (GRwt) or two different dimerization/transactivation-deficient GR mutant [A458T or hGR(D4X)] expression vectors. Treatment with dexamethasone (Dex, 0.1 µM) or solvent alone was performed 4 h after the transfection and the cells were harvested 48 h later for luciferase activity measurements. The results are expressed as the level of MKP-1 promoter-driven firefly luciferase expression after correcting for the transfection efficiency by Renilla luciferase measurements (relative luciferase activity), and are presented as the mean ± SD of three independent experiments.
    Second Strand Complementary Dna Cdna Synthesis, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs nebnext ultra ii non directional rna second strand synthesis module
    Fig. 3. Glucocorticoids induce MKP-1 gene expression. RBL-2H3 mast cells ( A ), mouse bone marrow-derived mast cells ( B ) and NIH-3T3 fibroblasts ( C ) were treated for the indicated time with dexamethasone (Dex, 0.1 µM) or solvent alone. Total <t>RNA</t> was extracted and subjected to northern blotting using <t>cDNA</t> probes for MKP-1 and GAPDH. ( D ) COS-7 cells were transiently co-transfected with an MKP-1 promoter firefly luciferase construct (-1716MKP-1luc) and a Renilla luciferase construct as an internal control, together with either wild-type glucocorticoid receptor (GRwt) or two different dimerization/transactivation-deficient GR mutant [A458T or hGR(D4X)] expression vectors. Treatment with dexamethasone (Dex, 0.1 µM) or solvent alone was performed 4 h after the transfection and the cells were harvested 48 h later for luciferase activity measurements. The results are expressed as the level of MKP-1 promoter-driven firefly luciferase expression after correcting for the transfection efficiency by Renilla luciferase measurements (relative luciferase activity), and are presented as the mean ± SD of three independent experiments.
    Nebnext Ultra Ii Non Directional Rna Second Strand Synthesis Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 361 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rnaseh dependent second strand cdna synthesis
    Fig. 3. Glucocorticoids induce MKP-1 gene expression. RBL-2H3 mast cells ( A ), mouse bone marrow-derived mast cells ( B ) and NIH-3T3 fibroblasts ( C ) were treated for the indicated time with dexamethasone (Dex, 0.1 µM) or solvent alone. Total <t>RNA</t> was extracted and subjected to northern blotting using <t>cDNA</t> probes for MKP-1 and GAPDH. ( D ) COS-7 cells were transiently co-transfected with an MKP-1 promoter firefly luciferase construct (-1716MKP-1luc) and a Renilla luciferase construct as an internal control, together with either wild-type glucocorticoid receptor (GRwt) or two different dimerization/transactivation-deficient GR mutant [A458T or hGR(D4X)] expression vectors. Treatment with dexamethasone (Dex, 0.1 µM) or solvent alone was performed 4 h after the transfection and the cells were harvested 48 h later for luciferase activity measurements. The results are expressed as the level of MKP-1 promoter-driven firefly luciferase expression after correcting for the transfection efficiency by Renilla luciferase measurements (relative luciferase activity), and are presented as the mean ± SD of three independent experiments.
    Rnaseh Dependent Second Strand Cdna Synthesis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ABM Inc second strand cdna synthesis kit
    Fig. 3. Glucocorticoids induce MKP-1 gene expression. RBL-2H3 mast cells ( A ), mouse bone marrow-derived mast cells ( B ) and NIH-3T3 fibroblasts ( C ) were treated for the indicated time with dexamethasone (Dex, 0.1 µM) or solvent alone. Total <t>RNA</t> was extracted and subjected to northern blotting using <t>cDNA</t> probes for MKP-1 and GAPDH. ( D ) COS-7 cells were transiently co-transfected with an MKP-1 promoter firefly luciferase construct (-1716MKP-1luc) and a Renilla luciferase construct as an internal control, together with either wild-type glucocorticoid receptor (GRwt) or two different dimerization/transactivation-deficient GR mutant [A458T or hGR(D4X)] expression vectors. Treatment with dexamethasone (Dex, 0.1 µM) or solvent alone was performed 4 h after the transfection and the cells were harvested 48 h later for luciferase activity measurements. The results are expressed as the level of MKP-1 promoter-driven firefly luciferase expression after correcting for the transfection efficiency by Renilla luciferase measurements (relative luciferase activity), and are presented as the mean ± SD of three independent experiments.
    Second Strand Cdna Synthesis Kit, supplied by ABM Inc, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rnase h dna polymerase i mediated second strand cdna synthesis
    Fig. 3. Glucocorticoids induce MKP-1 gene expression. RBL-2H3 mast cells ( A ), mouse bone marrow-derived mast cells ( B ) and NIH-3T3 fibroblasts ( C ) were treated for the indicated time with dexamethasone (Dex, 0.1 µM) or solvent alone. Total <t>RNA</t> was extracted and subjected to northern blotting using <t>cDNA</t> probes for MKP-1 and GAPDH. ( D ) COS-7 cells were transiently co-transfected with an MKP-1 promoter firefly luciferase construct (-1716MKP-1luc) and a Renilla luciferase construct as an internal control, together with either wild-type glucocorticoid receptor (GRwt) or two different dimerization/transactivation-deficient GR mutant [A458T or hGR(D4X)] expression vectors. Treatment with dexamethasone (Dex, 0.1 µM) or solvent alone was performed 4 h after the transfection and the cells were harvested 48 h later for luciferase activity measurements. The results are expressed as the level of MKP-1 promoter-driven firefly luciferase expression after correcting for the transfection efficiency by Renilla luciferase measurements (relative luciferase activity), and are presented as the mean ± SD of three independent experiments.
    Rnase H Dna Polymerase I Mediated Second Strand Cdna Synthesis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad iscript second strand cdna synthesis kit
    Fig. 3. Glucocorticoids induce MKP-1 gene expression. RBL-2H3 mast cells ( A ), mouse bone marrow-derived mast cells ( B ) and NIH-3T3 fibroblasts ( C ) were treated for the indicated time with dexamethasone (Dex, 0.1 µM) or solvent alone. Total <t>RNA</t> was extracted and subjected to northern blotting using <t>cDNA</t> probes for MKP-1 and GAPDH. ( D ) COS-7 cells were transiently co-transfected with an MKP-1 promoter firefly luciferase construct (-1716MKP-1luc) and a Renilla luciferase construct as an internal control, together with either wild-type glucocorticoid receptor (GRwt) or two different dimerization/transactivation-deficient GR mutant [A458T or hGR(D4X)] expression vectors. Treatment with dexamethasone (Dex, 0.1 µM) or solvent alone was performed 4 h after the transfection and the cells were harvested 48 h later for luciferase activity measurements. The results are expressed as the level of MKP-1 promoter-driven firefly luciferase expression after correcting for the transfection efficiency by Renilla luciferase measurements (relative luciferase activity), and are presented as the mean ± SD of three independent experiments.
    Iscript Second Strand Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs second strand cdna synthesis kit
    Fig. 3. Glucocorticoids induce MKP-1 gene expression. RBL-2H3 mast cells ( A ), mouse bone marrow-derived mast cells ( B ) and NIH-3T3 fibroblasts ( C ) were treated for the indicated time with dexamethasone (Dex, 0.1 µM) or solvent alone. Total <t>RNA</t> was extracted and subjected to northern blotting using <t>cDNA</t> probes for MKP-1 and GAPDH. ( D ) COS-7 cells were transiently co-transfected with an MKP-1 promoter firefly luciferase construct (-1716MKP-1luc) and a Renilla luciferase construct as an internal control, together with either wild-type glucocorticoid receptor (GRwt) or two different dimerization/transactivation-deficient GR mutant [A458T or hGR(D4X)] expression vectors. Treatment with dexamethasone (Dex, 0.1 µM) or solvent alone was performed 4 h after the transfection and the cells were harvested 48 h later for luciferase activity measurements. The results are expressed as the level of MKP-1 promoter-driven firefly luciferase expression after correcting for the transfection efficiency by Renilla luciferase measurements (relative luciferase activity), and are presented as the mean ± SD of three independent experiments.
    Second Strand Cdna Synthesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Fig. 3. Glucocorticoids induce MKP-1 gene expression. RBL-2H3 mast cells ( A ), mouse bone marrow-derived mast cells ( B ) and NIH-3T3 fibroblasts ( C ) were treated for the indicated time with dexamethasone (Dex, 0.1 µM) or solvent alone. Total RNA was extracted and subjected to northern blotting using cDNA probes for MKP-1 and GAPDH. ( D ) COS-7 cells were transiently co-transfected with an MKP-1 promoter firefly luciferase construct (-1716MKP-1luc) and a Renilla luciferase construct as an internal control, together with either wild-type glucocorticoid receptor (GRwt) or two different dimerization/transactivation-deficient GR mutant [A458T or hGR(D4X)] expression vectors. Treatment with dexamethasone (Dex, 0.1 µM) or solvent alone was performed 4 h after the transfection and the cells were harvested 48 h later for luciferase activity measurements. The results are expressed as the level of MKP-1 promoter-driven firefly luciferase expression after correcting for the transfection efficiency by Renilla luciferase measurements (relative luciferase activity), and are presented as the mean ± SD of three independent experiments.

    Journal: The EMBO Journal

    Article Title: Glucocorticoids inhibit MAP kinase via increased expression and decreased degradation of MKP-1

    doi: 10.1093/emboj/20.24.7108

    Figure Lengend Snippet: Fig. 3. Glucocorticoids induce MKP-1 gene expression. RBL-2H3 mast cells ( A ), mouse bone marrow-derived mast cells ( B ) and NIH-3T3 fibroblasts ( C ) were treated for the indicated time with dexamethasone (Dex, 0.1 µM) or solvent alone. Total RNA was extracted and subjected to northern blotting using cDNA probes for MKP-1 and GAPDH. ( D ) COS-7 cells were transiently co-transfected with an MKP-1 promoter firefly luciferase construct (-1716MKP-1luc) and a Renilla luciferase construct as an internal control, together with either wild-type glucocorticoid receptor (GRwt) or two different dimerization/transactivation-deficient GR mutant [A458T or hGR(D4X)] expression vectors. Treatment with dexamethasone (Dex, 0.1 µM) or solvent alone was performed 4 h after the transfection and the cells were harvested 48 h later for luciferase activity measurements. The results are expressed as the level of MKP-1 promoter-driven firefly luciferase expression after correcting for the transfection efficiency by Renilla luciferase measurements (relative luciferase activity), and are presented as the mean ± SD of three independent experiments.

    Article Snippet: A 3 µg aliquot of poly(A)+ RNA was used for first and second strand cDNA synthesis using an oligo(dT) primer harboring a T7 polymerase promoter sequence (Superscript Choice System; Life Technologies, Karlsruhe, Germany).

    Techniques: Expressing, Derivative Assay, Northern Blot, Transfection, Luciferase, Construct, Mutagenesis, Activity Assay