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    Thermo Fisher extracellular sdc 1 expression
    Proliferation and cytokine production are reduced in <t>Sdc-1</t> deficient splenocytes upon stimulation with ConA. Proliferation of WT and Sdc-1 deficient splenocytes was determined in the presence of 0.1–0.25 μg/ml ConA as analyzed by CFSE dilution using flow cytometry (A). Interferon-γ, IL-17 and IL-10 production by WT and Sdc-1 deficient splenocytes was determined in the presence of 0.25 μg/ml ConA (B), as determined by ELISA. Proliferation and cytokine levels are expressed as means and standard error of means of 10 independent experiments. * p
    Extracellular Sdc 1 Expression, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Bio-Rad gene exp cxcr4 hs00607978 s1
    Proliferation and cytokine production are reduced in <t>Sdc-1</t> deficient splenocytes upon stimulation with ConA. Proliferation of WT and Sdc-1 deficient splenocytes was determined in the presence of 0.1–0.25 μg/ml ConA as analyzed by CFSE dilution using flow cytometry (A). Interferon-γ, IL-17 and IL-10 production by WT and Sdc-1 deficient splenocytes was determined in the presence of 0.25 μg/ml ConA (B), as determined by ELISA. Proliferation and cytokine levels are expressed as means and standard error of means of 10 independent experiments. * p
    Gene Exp Cxcr4 Hs00607978 S1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp cxcr4 hs00607978 s1/product/Bio-Rad
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    Proliferation and cytokine production are reduced in Sdc-1 deficient splenocytes upon stimulation with ConA. Proliferation of WT and Sdc-1 deficient splenocytes was determined in the presence of 0.1–0.25 μg/ml ConA as analyzed by CFSE dilution using flow cytometry (A). Interferon-γ, IL-17 and IL-10 production by WT and Sdc-1 deficient splenocytes was determined in the presence of 0.25 μg/ml ConA (B), as determined by ELISA. Proliferation and cytokine levels are expressed as means and standard error of means of 10 independent experiments. * p

    Journal: PLoS ONE

    Article Title: Role of syndecan-1 in the interaction between dendritic cells and T cells

    doi: 10.1371/journal.pone.0230835

    Figure Lengend Snippet: Proliferation and cytokine production are reduced in Sdc-1 deficient splenocytes upon stimulation with ConA. Proliferation of WT and Sdc-1 deficient splenocytes was determined in the presence of 0.1–0.25 μg/ml ConA as analyzed by CFSE dilution using flow cytometry (A). Interferon-γ, IL-17 and IL-10 production by WT and Sdc-1 deficient splenocytes was determined in the presence of 0.25 μg/ml ConA (B), as determined by ELISA. Proliferation and cytokine levels are expressed as means and standard error of means of 10 independent experiments. * p

    Article Snippet: T cells and DC were stained with anti-syndecan-1 (rat IgG2A κ monoclonal antibody, clone 281–2, BD Biosciences) or isotype control (rat IgG2Aκ, BD Biosciences) and conjugate (Alexa488, goat anti rat, Molecular Probes, Eugene, USA) to analyze extracellular Sdc-1 expression.

    Techniques: Flow Cytometry, Enzyme-linked Immunosorbent Assay

    Sdc-1 deficient T cells display a reduced proliferative response in co-culture with DC. Sdc-1 expression at the cell membrane of naïve WT (A, left panel, black line) and Sdc-1-deficient splenic T cells (A, left panel, grey area), and intracellular and cell surface expression of ConA (0.25 μg/ml) -activated WT (A, right panel, black line) and Sdc-1 deficient T cells (A, right panel, grey area). T cell proliferation in co-cultures of WT and Sdc-1 deficient T cells with unstimulated DC (B, left panel) or LPS matured DC (B, right panel) as analyzed by CFSE dilution using flow cytometry. IFN-γ (C), TNF-α (D) and IL-17 (E) production in co-cultures of WT and Sdc-1 deficient T cells with either unstimulated DC (C, D, E, left panels) or LPS matured DC (C, D, E, right panels) as measured by ELISA. Control conditions with T cells only showed only minimal proliferation (max. 2–4% at day 6). Expression of proliferation and cytokine levels reflect means and standard error of means of 4 independent experiments. Mann Whitney test, * p

    Journal: PLoS ONE

    Article Title: Role of syndecan-1 in the interaction between dendritic cells and T cells

    doi: 10.1371/journal.pone.0230835

    Figure Lengend Snippet: Sdc-1 deficient T cells display a reduced proliferative response in co-culture with DC. Sdc-1 expression at the cell membrane of naïve WT (A, left panel, black line) and Sdc-1-deficient splenic T cells (A, left panel, grey area), and intracellular and cell surface expression of ConA (0.25 μg/ml) -activated WT (A, right panel, black line) and Sdc-1 deficient T cells (A, right panel, grey area). T cell proliferation in co-cultures of WT and Sdc-1 deficient T cells with unstimulated DC (B, left panel) or LPS matured DC (B, right panel) as analyzed by CFSE dilution using flow cytometry. IFN-γ (C), TNF-α (D) and IL-17 (E) production in co-cultures of WT and Sdc-1 deficient T cells with either unstimulated DC (C, D, E, left panels) or LPS matured DC (C, D, E, right panels) as measured by ELISA. Control conditions with T cells only showed only minimal proliferation (max. 2–4% at day 6). Expression of proliferation and cytokine levels reflect means and standard error of means of 4 independent experiments. Mann Whitney test, * p

    Article Snippet: T cells and DC were stained with anti-syndecan-1 (rat IgG2A κ monoclonal antibody, clone 281–2, BD Biosciences) or isotype control (rat IgG2Aκ, BD Biosciences) and conjugate (Alexa488, goat anti rat, Molecular Probes, Eugene, USA) to analyze extracellular Sdc-1 expression.

    Techniques: Co-Culture Assay, Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    Sdc-1 deficiency affects cytokine levels in allograft recipients without affecting allograft survival. Allograft survival in a fully mismatched heterotopic heart transplantation model. Hearts were obtained from Sdc-1-deficient (n = 9) or WT mice (n = 8) and transplanted in Balb/c mice (A). Plasma cytokine levels in Balb/c recipient mice that received WT or Sdc-1 deficient hearts (B). Allograft survival of Balb/c hearts in WT (n = 8) or Sdc1-deficient (n = 8) recipients (C). Plasma cytokine levels in WT or Sdc-1 deficient recipients that received a Balb/c heart (D). * p

    Journal: PLoS ONE

    Article Title: Role of syndecan-1 in the interaction between dendritic cells and T cells

    doi: 10.1371/journal.pone.0230835

    Figure Lengend Snippet: Sdc-1 deficiency affects cytokine levels in allograft recipients without affecting allograft survival. Allograft survival in a fully mismatched heterotopic heart transplantation model. Hearts were obtained from Sdc-1-deficient (n = 9) or WT mice (n = 8) and transplanted in Balb/c mice (A). Plasma cytokine levels in Balb/c recipient mice that received WT or Sdc-1 deficient hearts (B). Allograft survival of Balb/c hearts in WT (n = 8) or Sdc1-deficient (n = 8) recipients (C). Plasma cytokine levels in WT or Sdc-1 deficient recipients that received a Balb/c heart (D). * p

    Article Snippet: T cells and DC were stained with anti-syndecan-1 (rat IgG2A κ monoclonal antibody, clone 281–2, BD Biosciences) or isotype control (rat IgG2Aκ, BD Biosciences) and conjugate (Alexa488, goat anti rat, Molecular Probes, Eugene, USA) to analyze extracellular Sdc-1 expression.

    Techniques: Transplantation Assay, Mouse Assay

    Sdc-1 deficiency does not affect DC maturation or function. Sdc-1 expression at the cell surface (A, left panel) or intracellularly and at the cell surface (A, right panel) by unstimulated and LPS matured WT (black line) and Sdc-1-deficient (grey area) DC as measured by flow cytometry (A). Expression of co-stimulatory molecules and MHCII by unstimulated (B, left panel) or LPS matured (B, right panel) WT and Sdc-1-deficient CD11c + DC as measured by flow cytometry (B). Cytokine profile of unstimulated (C, left panel) or LPS matured (C, right panel) WT and Sdc-1-deficient DC as measured by ELISA. The increase in expression of co-stimulatory molecules and in DC derived cytokine and CXCL1 production upon LPS exposure is significant compared to unstimulated DC. T cell stimulatory capacity of unstimulated (D, left panel) or LPS matured (D, right panel) WT and Sdc-1-deficient DC as measured by CFSE dilution using flow cytometry. T cell derived cytokine production in co-cultures of T cell and unstimulated (E, left panel) or LPS maturated (E, right panel) WT and Sdc-1-deficient DC as measured by ELISA. Levels of IL-4 and TNF-α were undetectable. All experiments were replicated 3–5 times. Results are expressed as means ± standard error of means. * p

    Journal: PLoS ONE

    Article Title: Role of syndecan-1 in the interaction between dendritic cells and T cells

    doi: 10.1371/journal.pone.0230835

    Figure Lengend Snippet: Sdc-1 deficiency does not affect DC maturation or function. Sdc-1 expression at the cell surface (A, left panel) or intracellularly and at the cell surface (A, right panel) by unstimulated and LPS matured WT (black line) and Sdc-1-deficient (grey area) DC as measured by flow cytometry (A). Expression of co-stimulatory molecules and MHCII by unstimulated (B, left panel) or LPS matured (B, right panel) WT and Sdc-1-deficient CD11c + DC as measured by flow cytometry (B). Cytokine profile of unstimulated (C, left panel) or LPS matured (C, right panel) WT and Sdc-1-deficient DC as measured by ELISA. The increase in expression of co-stimulatory molecules and in DC derived cytokine and CXCL1 production upon LPS exposure is significant compared to unstimulated DC. T cell stimulatory capacity of unstimulated (D, left panel) or LPS matured (D, right panel) WT and Sdc-1-deficient DC as measured by CFSE dilution using flow cytometry. T cell derived cytokine production in co-cultures of T cell and unstimulated (E, left panel) or LPS maturated (E, right panel) WT and Sdc-1-deficient DC as measured by ELISA. Levels of IL-4 and TNF-α were undetectable. All experiments were replicated 3–5 times. Results are expressed as means ± standard error of means. * p

    Article Snippet: T cells and DC were stained with anti-syndecan-1 (rat IgG2A κ monoclonal antibody, clone 281–2, BD Biosciences) or isotype control (rat IgG2Aκ, BD Biosciences) and conjugate (Alexa488, goat anti rat, Molecular Probes, Eugene, USA) to analyze extracellular Sdc-1 expression.

    Techniques: Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Derivative Assay