Article Title: Role of syndecan-1 in the interaction between dendritic cells and T cells
Figure Lengend Snippet: Sdc-1 deficient T cells display a reduced proliferative response in co-culture with DC. Sdc-1 expression at the cell membrane of naïve WT (A, left panel, black line) and Sdc-1-deficient splenic T cells (A, left panel, grey area), and intracellular and cell surface expression of ConA (0.25 µg/ml)-activated WT (A, right panel, black line) and Sdc-1 deficient T cells (A, right panel, grey area). T cell proliferation in co-cultures of WT and Sdc-1 deficient T cells with unstimulated DC (B, left panel) or LPS matured DC (B, right panel) as analyzed by CFSE dilution using flow cytometry. IFN-γ (C), TNF-α (D) and IL-17 (E) production in co-cultures of WT and Sdc-1 deficient T cells with either unstimulated DC (C, D, E, left panels) or LPS matured DC (C, D, E, right panels) as measured by ELISA. Expression of proliferation and cytokine levels reflect means and standard error of means of 4 independent experiments. Mann Whitney test, * p
Article Snippet: T cells and DC were stained with anti-syndecan-1 (rat IgG2A κ monoclonal antibody, clone 281-2, BD Biosciences) or isotype control (rat IgG2A κ, BD Biosciences) and conjugate (Alexa488, goat anti rat, Molecular Probes, Eugene, USA) to analyze extracellular Sdc-1 expression.
Techniques: Co-Culture Assay, Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY