sdc expression Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 84
    Thermo Fisher extracellular sdc 1 expression
    Proliferation and cytokine production are reduced in <t>Sdc-1</t> deficient splenocytes upon stimulation with ConA. Proliferation of WT and Sdc-1 deficient splenocytes was determined in the presence of 0.1 - 0.25 µg/ml ConA as analyzed by CFSE dilution using flow cytometry (A). Interferon-γ, IL-17 and IL-10 production by WT and Sdc-1 deficient splenocytes was determined in the presence of 0.25 µg/ml ConA (B), as determined by ELISA. Proliferation and cytokine levels are expressed as means and standard error of means of 10 independent experiments. * p
    Extracellular Sdc 1 Expression, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/extracellular sdc 1 expression/product/Thermo Fisher
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    extracellular sdc 1 expression - by Bioz Stars, 2020-09
    84/100 stars
      Buy from Supplier

    Image Search Results


    Proliferation and cytokine production are reduced in Sdc-1 deficient splenocytes upon stimulation with ConA. Proliferation of WT and Sdc-1 deficient splenocytes was determined in the presence of 0.1 - 0.25 µg/ml ConA as analyzed by CFSE dilution using flow cytometry (A). Interferon-γ, IL-17 and IL-10 production by WT and Sdc-1 deficient splenocytes was determined in the presence of 0.25 µg/ml ConA (B), as determined by ELISA. Proliferation and cytokine levels are expressed as means and standard error of means of 10 independent experiments. * p

    Journal: bioRxiv

    Article Title: Role of syndecan-1 in the interaction between dendritic cells and T cells

    doi: 10.1101/2020.03.11.987602

    Figure Lengend Snippet: Proliferation and cytokine production are reduced in Sdc-1 deficient splenocytes upon stimulation with ConA. Proliferation of WT and Sdc-1 deficient splenocytes was determined in the presence of 0.1 - 0.25 µg/ml ConA as analyzed by CFSE dilution using flow cytometry (A). Interferon-γ, IL-17 and IL-10 production by WT and Sdc-1 deficient splenocytes was determined in the presence of 0.25 µg/ml ConA (B), as determined by ELISA. Proliferation and cytokine levels are expressed as means and standard error of means of 10 independent experiments. * p

    Article Snippet: T cells and DC were stained with anti-syndecan-1 (rat IgG2A κ monoclonal antibody, clone 281-2, BD Biosciences) or isotype control (rat IgG2A κ, BD Biosciences) and conjugate (Alexa488, goat anti rat, Molecular Probes, Eugene, USA) to analyze extracellular Sdc-1 expression.

    Techniques: Flow Cytometry, Enzyme-linked Immunosorbent Assay

    Sdc-1 deficiency affects cytokine levels in allograft recipients without affecting allograft survival. Allograft survival in a fully mismatched heterotopic heart transplantation model. Hearts were obtained from Sdc-1-deficient (n=9) or WT mice (n=8) and transplanted in Balb/c mice (A). Plasma cytokine levels in Balb/c recipient mice that received WT or Sdc-1 deficient hearts (B). Allograft survival of Balb/c hearts in WT (n=8) or Sdc1-deficient (n=8) recipients (C). Plasma cytokine levels in WT or Sdc-1 deficient recipients that received a Balb/c heart (D). * p

    Journal: bioRxiv

    Article Title: Role of syndecan-1 in the interaction between dendritic cells and T cells

    doi: 10.1101/2020.03.11.987602

    Figure Lengend Snippet: Sdc-1 deficiency affects cytokine levels in allograft recipients without affecting allograft survival. Allograft survival in a fully mismatched heterotopic heart transplantation model. Hearts were obtained from Sdc-1-deficient (n=9) or WT mice (n=8) and transplanted in Balb/c mice (A). Plasma cytokine levels in Balb/c recipient mice that received WT or Sdc-1 deficient hearts (B). Allograft survival of Balb/c hearts in WT (n=8) or Sdc1-deficient (n=8) recipients (C). Plasma cytokine levels in WT or Sdc-1 deficient recipients that received a Balb/c heart (D). * p

    Article Snippet: T cells and DC were stained with anti-syndecan-1 (rat IgG2A κ monoclonal antibody, clone 281-2, BD Biosciences) or isotype control (rat IgG2A κ, BD Biosciences) and conjugate (Alexa488, goat anti rat, Molecular Probes, Eugene, USA) to analyze extracellular Sdc-1 expression.

    Techniques: Transplantation Assay, Mouse Assay

    Sdc-1 deficient T cells display a reduced proliferative response in co-culture with DC. Sdc-1 expression at the cell membrane of naïve WT (A, left panel, black line) and Sdc-1-deficient splenic T cells (A, left panel, grey area), and intracellular and cell surface expression of ConA (0.25 µg/ml)-activated WT (A, right panel, black line) and Sdc-1 deficient T cells (A, right panel, grey area). T cell proliferation in co-cultures of WT and Sdc-1 deficient T cells with unstimulated DC (B, left panel) or LPS matured DC (B, right panel) as analyzed by CFSE dilution using flow cytometry. IFN-γ (C), TNF-α (D) and IL-17 (E) production in co-cultures of WT and Sdc-1 deficient T cells with either unstimulated DC (C, D, E, left panels) or LPS matured DC (C, D, E, right panels) as measured by ELISA. Expression of proliferation and cytokine levels reflect means and standard error of means of 4 independent experiments. Mann Whitney test, * p

    Journal: bioRxiv

    Article Title: Role of syndecan-1 in the interaction between dendritic cells and T cells

    doi: 10.1101/2020.03.11.987602

    Figure Lengend Snippet: Sdc-1 deficient T cells display a reduced proliferative response in co-culture with DC. Sdc-1 expression at the cell membrane of naïve WT (A, left panel, black line) and Sdc-1-deficient splenic T cells (A, left panel, grey area), and intracellular and cell surface expression of ConA (0.25 µg/ml)-activated WT (A, right panel, black line) and Sdc-1 deficient T cells (A, right panel, grey area). T cell proliferation in co-cultures of WT and Sdc-1 deficient T cells with unstimulated DC (B, left panel) or LPS matured DC (B, right panel) as analyzed by CFSE dilution using flow cytometry. IFN-γ (C), TNF-α (D) and IL-17 (E) production in co-cultures of WT and Sdc-1 deficient T cells with either unstimulated DC (C, D, E, left panels) or LPS matured DC (C, D, E, right panels) as measured by ELISA. Expression of proliferation and cytokine levels reflect means and standard error of means of 4 independent experiments. Mann Whitney test, * p

    Article Snippet: T cells and DC were stained with anti-syndecan-1 (rat IgG2A κ monoclonal antibody, clone 281-2, BD Biosciences) or isotype control (rat IgG2A κ, BD Biosciences) and conjugate (Alexa488, goat anti rat, Molecular Probes, Eugene, USA) to analyze extracellular Sdc-1 expression.

    Techniques: Co-Culture Assay, Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    Sdc-1 deficiency does not affect DC maturation or function. Sdc-1 expression at the cell surface (A, left panel) or intracellularly and at the cell surface (A, right panel) by unstimulated and LPS matured WT (black line) and Sdc-1-deficient (grey area) DC as measured by flow cytometry (A). Expression of co-stimulatory molecules and MHCII by unstimulated (B, left panel) or LPS matured (B, right panel) WT and Sdc-1-deficient CD11c + DC as measured by flow cytometry (B). Cytokine profile of unstimulated (C, left panel) or LPS matured (C, right panel) WT and Sdc-1-deficient DC as measured by ELISA. The increase in expression of co-stimulatory molecules and in DC derived cytokine and CXCL1 production upon LPS exposure is significant compared to unstimulated DC. T cell stimulatory capacity of unstimulated (D, left panel) or LPS matured (D, right panel) WT and Sdc-1-deficient DC as measured by CFSE dilution using flow cytometry. T cell derived cytokine production in co-cultures of T cell and unstimulated (E, left panel) or LPS maturated (E, right panel) WT and Sdc-1-deficient DC as measured by ELISA. Levels of IL-4 and TNF-α were undetectable. All experiments were replicated 3-5 times. Results are expressed as means ± standard error of means. * p

    Journal: bioRxiv

    Article Title: Role of syndecan-1 in the interaction between dendritic cells and T cells

    doi: 10.1101/2020.03.11.987602

    Figure Lengend Snippet: Sdc-1 deficiency does not affect DC maturation or function. Sdc-1 expression at the cell surface (A, left panel) or intracellularly and at the cell surface (A, right panel) by unstimulated and LPS matured WT (black line) and Sdc-1-deficient (grey area) DC as measured by flow cytometry (A). Expression of co-stimulatory molecules and MHCII by unstimulated (B, left panel) or LPS matured (B, right panel) WT and Sdc-1-deficient CD11c + DC as measured by flow cytometry (B). Cytokine profile of unstimulated (C, left panel) or LPS matured (C, right panel) WT and Sdc-1-deficient DC as measured by ELISA. The increase in expression of co-stimulatory molecules and in DC derived cytokine and CXCL1 production upon LPS exposure is significant compared to unstimulated DC. T cell stimulatory capacity of unstimulated (D, left panel) or LPS matured (D, right panel) WT and Sdc-1-deficient DC as measured by CFSE dilution using flow cytometry. T cell derived cytokine production in co-cultures of T cell and unstimulated (E, left panel) or LPS maturated (E, right panel) WT and Sdc-1-deficient DC as measured by ELISA. Levels of IL-4 and TNF-α were undetectable. All experiments were replicated 3-5 times. Results are expressed as means ± standard error of means. * p

    Article Snippet: T cells and DC were stained with anti-syndecan-1 (rat IgG2A κ monoclonal antibody, clone 281-2, BD Biosciences) or isotype control (rat IgG2A κ, BD Biosciences) and conjugate (Alexa488, goat anti rat, Molecular Probes, Eugene, USA) to analyze extracellular Sdc-1 expression.

    Techniques: Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Derivative Assay