scd40l Search Results


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    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human soluble CD40L 96wells kit with removable strips
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    99
    Thermo Fisher human scd40l platinum elisa kit
    Human Scd40l Platinum Elisa Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Bender MedSystems scd40l
    <t>sCD40L</t> levels in before and after surgery, values are mean ± S.D. *P
    Scd40l, supplied by Bender MedSystems, used in various techniques. Bioz Stars score: 92/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher scd40l
    B lymphocytes from cancer patients can be activated by treatment with <t>sCD40L</t> and IL-4. Viable 90% enriched B lymphocytes from PBMCs from cervical cancer patients (cancer) or controls (control), were incubated with 10 μg/ml sCD40L and 50 ng/ml IL-4 for 24 hours. After harvesting, cells were labeled with anti-CD19, CD80, CD86 and HLA-DR and analyzed by flow cytometry, where 10 5 events were acquired in a FACSCanto (BD Biosciences, Carlsbad, CA). For data analyzes, we excluded debris and doublets and gated the MHC-II, CD80 + and CD86 + populations on the CD19 + cells. We compared B lymphocytes from cancer patients (cancer) and controls (control), and within these two groups, sCD40L and IL-4 treated (+) and untreated cells (-). (A) Expression of MHC-II shown as median of fluorescence intensity (MFI); 100% of the cells were positive for MHC-II expression. (B) Percentage of the CD80 + CD86 + population. Bars indicate groups with significant different results, based on ANOVA two-way test, where p
    Scd40l, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Diaclone scd 40l
    Serum inflammatory biomarkers levels in healthy subjects and across various rheumatic AF groups. AF: atrial fibrillation, hs‐CRP: high sensitivity C‐reactive protein, IL‐6: interleukin‐6, <t>sCD‐40L:</t> soluble CD‐40 Ligand. SI unit: mg (milligram); pg (picogram); ng (nanogram); l(liter); ml(milliliter)
    Scd 40l, supplied by Diaclone, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bender MedSystems soluble cd40 ligand scd40l
    Serum inflammatory biomarkers levels in healthy subjects and across various rheumatic AF groups. AF: atrial fibrillation, hs‐CRP: high sensitivity C‐reactive protein, IL‐6: interleukin‐6, <t>sCD‐40L:</t> soluble CD‐40 Ligand. SI unit: mg (milligram); pg (picogram); ng (nanogram); l(liter); ml(milliliter)
    Soluble Cd40 Ligand Scd40l, supplied by Bender MedSystems, used in various techniques. Bioz Stars score: 88/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Abcam anti cd40l antibody
    Serum inflammatory biomarkers levels in healthy subjects and across various rheumatic AF groups. AF: atrial fibrillation, hs‐CRP: high sensitivity C‐reactive protein, IL‐6: interleukin‐6, <t>sCD‐40L:</t> soluble CD‐40 Ligand. SI unit: mg (milligram); pg (picogram); ng (nanogram); l(liter); ml(milliliter)
    Anti Cd40l Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems recombinant human scd40l
    Serum inflammatory biomarkers levels in healthy subjects and across various rheumatic AF groups. AF: atrial fibrillation, hs‐CRP: high sensitivity C‐reactive protein, IL‐6: interleukin‐6, <t>sCD‐40L:</t> soluble CD‐40 Ligand. SI unit: mg (milligram); pg (picogram); ng (nanogram); l(liter); ml(milliliter)
    Recombinant Human Scd40l, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Ancell corporation biotinylated mouse anti human scd40l antibody
    Serum inflammatory biomarkers levels in healthy subjects and across various rheumatic AF groups. AF: atrial fibrillation, hs‐CRP: high sensitivity C‐reactive protein, IL‐6: interleukin‐6, <t>sCD‐40L:</t> soluble CD‐40 Ligand. SI unit: mg (milligram); pg (picogram); ng (nanogram); l(liter); ml(milliliter)
    Biotinylated Mouse Anti Human Scd40l Antibody, supplied by Ancell corporation, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Bender MedSystems mouse scd40l elisa kit
    HIV-1 Tat induces platelet activation in vivo , which is abolished in the presence of VPA (A) The plasma concentration of <t>sCD40L</t> in C57BL/6 mice was measured via <t>ELISA.</t> Upon intravenous injection with HIV-1 Tat, there is a significant increase in the level of sCD40L found in the plasma; however, this is inhibited, to levels that mimic those of mice receiving saline, when VPA is administered once a day for three days prior to injection of Tat. (B) Platelet activation was verified in the samples described in panel A by ELISA specific for mouse platelet factor 4 (PF4). N=4 for each treatment group, labeled as intraperitoneal pretreatment/intravenous treatment. The concentrations of sCD40L (pg/mL) and PF4 (ng/mL) are presented as mean (± SEM) of three replicates for each sample. Statistical analysis was performed by one-way ANOVA followed by Bonferroni's test for multiple comparisons; *denotes p
    Mouse Scd40l Elisa Kit, supplied by Bender MedSystems, used in various techniques. Bioz Stars score: 85/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher mouse scd40l platinium elisa kit
    HIV-1 Tat induces platelet activation in vivo , which is abolished in the presence of VPA (A) The plasma concentration of <t>sCD40L</t> in C57BL/6 mice was measured via <t>ELISA.</t> Upon intravenous injection with HIV-1 Tat, there is a significant increase in the level of sCD40L found in the plasma; however, this is inhibited, to levels that mimic those of mice receiving saline, when VPA is administered once a day for three days prior to injection of Tat. (B) Platelet activation was verified in the samples described in panel A by ELISA specific for mouse platelet factor 4 (PF4). N=4 for each treatment group, labeled as intraperitoneal pretreatment/intravenous treatment. The concentrations of sCD40L (pg/mL) and PF4 (ng/mL) are presented as mean (± SEM) of three replicates for each sample. Statistical analysis was performed by one-way ANOVA followed by Bonferroni's test for multiple comparisons; *denotes p
    Mouse Scd40l Platinium Elisa Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher rh scd40l
    HIV-1 Tat induces platelet activation in vivo , which is abolished in the presence of VPA (A) The plasma concentration of <t>sCD40L</t> in C57BL/6 mice was measured via <t>ELISA.</t> Upon intravenous injection with HIV-1 Tat, there is a significant increase in the level of sCD40L found in the plasma; however, this is inhibited, to levels that mimic those of mice receiving saline, when VPA is administered once a day for three days prior to injection of Tat. (B) Platelet activation was verified in the samples described in panel A by ELISA specific for mouse platelet factor 4 (PF4). N=4 for each treatment group, labeled as intraperitoneal pretreatment/intravenous treatment. The concentrations of sCD40L (pg/mL) and PF4 (ng/mL) are presented as mean (± SEM) of three replicates for each sample. Statistical analysis was performed by one-way ANOVA followed by Bonferroni's test for multiple comparisons; *denotes p
    Rh Scd40l, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    ImmunoTools antibodies scd40l
    <t>sCD40L</t> induced signaling is not significantly affected by cryopreservation. B cells from the same two healthy donors were analyzed before (fresh) and after (cryo) cryopreservation. The cells were stimulated with sCD40L (400 ng/mL) for 5 and 10 min before fixation and phospho flow analysis as described in Methods. Results are shown as mean ± standard deviation. Statistical significance was calculated between the two groups (fresh, cryo) by an ordinary two-way ANOVA with Sidak’s multiple comparisons test. ** p
    Antibodies Scd40l, supplied by ImmunoTools, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bender MedSystems recombinant human scd40l standards
    Association between UFP and <t>sCD40L,</t> platelets and leukocytes, 6-hour periods and lag 0 (0–23 hours) . sCD40L: soluble CD40Ligand. UFP: ultrafine particles (number concentration of particles with a size range of 0.01 to 0.1 μm).
    Recombinant Human Scd40l Standards, supplied by Bender MedSystems, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Autogen-Bioclear recombinant human scd40l
    Association between UFP and <t>sCD40L,</t> platelets and leukocytes, 6-hour periods and lag 0 (0–23 hours) . sCD40L: soluble CD40Ligand. UFP: ultrafine particles (number concentration of particles with a size range of 0.01 to 0.1 μm).
    Recombinant Human Scd40l, supplied by Autogen-Bioclear, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ImmunoTools recombinant human scd40l
    Association between UFP and <t>sCD40L,</t> platelets and leukocytes, 6-hour periods and lag 0 (0–23 hours) . sCD40L: soluble CD40Ligand. UFP: ultrafine particles (number concentration of particles with a size range of 0.01 to 0.1 μm).
    Recombinant Human Scd40l, supplied by ImmunoTools, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rdi research diagnostics recombinant human soluble cd40 ligand scd40l
    Association between UFP and <t>sCD40L,</t> platelets and leukocytes, 6-hour periods and lag 0 (0–23 hours) . sCD40L: soluble CD40Ligand. UFP: ultrafine particles (number concentration of particles with a size range of 0.01 to 0.1 μm).
    Recombinant Human Soluble Cd40 Ligand Scd40l, supplied by rdi research diagnostics, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PeproTech recombinant soluble cd40 ligand scd40l
    Association between UFP and <t>sCD40L,</t> platelets and leukocytes, 6-hour periods and lag 0 (0–23 hours) . sCD40L: soluble CD40Ligand. UFP: ultrafine particles (number concentration of particles with a size range of 0.01 to 0.1 μm).
    Recombinant Soluble Cd40 Ligand Scd40l, supplied by PeproTech, used in various techniques. Bioz Stars score: 95/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bender MedSystems human scd40l bms293
    Association between UFP and <t>sCD40L,</t> platelets and leukocytes, 6-hour periods and lag 0 (0–23 hours) . sCD40L: soluble CD40Ligand. UFP: ultrafine particles (number concentration of particles with a size range of 0.01 to 0.1 μm).
    Human Scd40l Bms293, supplied by Bender MedSystems, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems human scd40l tnfsf5
    Association between UFP and <t>sCD40L,</t> platelets and leukocytes, 6-hour periods and lag 0 (0–23 hours) . sCD40L: soluble CD40Ligand. UFP: ultrafine particles (number concentration of particles with a size range of 0.01 to 0.1 μm).
    Human Scd40l Tnfsf5, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bender MedSystems human scd40l kit
    Association between UFP and <t>sCD40L,</t> platelets and leukocytes, 6-hour periods and lag 0 (0–23 hours) . sCD40L: soluble CD40Ligand. UFP: ultrafine particles (number concentration of particles with a size range of 0.01 to 0.1 μm).
    Human Scd40l Kit, supplied by Bender MedSystems, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bender MedSystems human scd40l module set
    Effect of soluble CD40 ligand <t>(sCD40L)</t> on the cytokine profile. Peripheral blood mononuclear cells (PBMC) of HIV-infected patients (pat.) and control (ctrl.) donors were exposed to low-dose sCD40L (50 pg/ml) for 48 hours and subsequently stimulated with the CpG-P oligodeoxynucleotide 21798 for 20 hours. The levels of interleukin (IL-)2, interferon gamma (IFN-g), tumor necrosis factor alpha (TNF-a), IL-6, IL-8, and IL-10 were measured using a fluorescent bead assay. IL-4, IL-5, IL-12, TNF-beta, and IL-1 beta were not detected. Data are presented as mean and standard error. *p
    Human Scd40l Module Set, supplied by Bender MedSystems, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PeproTech human soluble cd40 ligand scd40l elisa development kit
    Effect of soluble CD40 ligand <t>(sCD40L)</t> on the cytokine profile. Peripheral blood mononuclear cells (PBMC) of HIV-infected patients (pat.) and control (ctrl.) donors were exposed to low-dose sCD40L (50 pg/ml) for 48 hours and subsequently stimulated with the CpG-P oligodeoxynucleotide 21798 for 20 hours. The levels of interleukin (IL-)2, interferon gamma (IFN-g), tumor necrosis factor alpha (TNF-a), IL-6, IL-8, and IL-10 were measured using a fluorescent bead assay. IL-4, IL-5, IL-12, TNF-beta, and IL-1 beta were not detected. Data are presented as mean and standard error. *p
    Human Soluble Cd40 Ligand Scd40l Elisa Development Kit, supplied by PeproTech, used in various techniques. Bioz Stars score: 95/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher human soluble cd40l scd40l extra sensitive platinum enzyme linked immunosorbent assay
    Effect of soluble CD40 ligand <t>(sCD40L)</t> on the cytokine profile. Peripheral blood mononuclear cells (PBMC) of HIV-infected patients (pat.) and control (ctrl.) donors were exposed to low-dose sCD40L (50 pg/ml) for 48 hours and subsequently stimulated with the CpG-P oligodeoxynucleotide 21798 for 20 hours. The levels of interleukin (IL-)2, interferon gamma (IFN-g), tumor necrosis factor alpha (TNF-a), IL-6, IL-8, and IL-10 were measured using a fluorescent bead assay. IL-4, IL-5, IL-12, TNF-beta, and IL-1 beta were not detected. Data are presented as mean and standard error. *p
    Human Soluble Cd40l Scd40l Extra Sensitive Platinum Enzyme Linked Immunosorbent Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    N/A
    Source Escherichia Coli CD40LG Human Recombinant fused with a 20 amino acid His tag at N terminus produced in E Coli is a single non glycosylated polypeptide chain containing 169
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    N/A
    Source Escherichia Coli sCD40 Human Recombinant produced in E Coli is a non glycosylated Polypeptide chain containing 149 amino acids and having a molecular mass of 16308 Dalton The sCD40
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    N/A
    Mouse monoclonal to sCD40L Isotype Note IgG Host Note Mouse Conjugation Note Unconjugated Reactivity Note Human
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    Image Search Results


    sCD40L levels in before and after surgery, values are mean ± S.D. *P

    Journal: International Journal of Molecular Sciences

    Article Title: Expression of CD40 and CD40L in Gastric Cancer Tissue and Its Clinical Significance

    doi: 10.3390/ijms10093900

    Figure Lengend Snippet: sCD40L levels in before and after surgery, values are mean ± S.D. *P

    Article Snippet: Enzyme Immunoassays Specific immunoassays for sCD40L (sCD40 detection limit, 95 pg/mL; with intra- and interassay CVs < 10% at different levels of sCD40L, Bender Medsystems) was measured using a commercial immunoassay kit (Bender MedSystems, Vienna, Austria).

    Techniques:

    sCD40L levels in three different groups, values are mean ±S.D. *P

    Journal: International Journal of Molecular Sciences

    Article Title: Expression of CD40 and CD40L in Gastric Cancer Tissue and Its Clinical Significance

    doi: 10.3390/ijms10093900

    Figure Lengend Snippet: sCD40L levels in three different groups, values are mean ±S.D. *P

    Article Snippet: Enzyme Immunoassays Specific immunoassays for sCD40L (sCD40 detection limit, 95 pg/mL; with intra- and interassay CVs < 10% at different levels of sCD40L, Bender Medsystems) was measured using a commercial immunoassay kit (Bender MedSystems, Vienna, Austria).

    Techniques:

    B lymphocytes from cancer patients can be activated by treatment with sCD40L and IL-4. Viable 90% enriched B lymphocytes from PBMCs from cervical cancer patients (cancer) or controls (control), were incubated with 10 μg/ml sCD40L and 50 ng/ml IL-4 for 24 hours. After harvesting, cells were labeled with anti-CD19, CD80, CD86 and HLA-DR and analyzed by flow cytometry, where 10 5 events were acquired in a FACSCanto (BD Biosciences, Carlsbad, CA). For data analyzes, we excluded debris and doublets and gated the MHC-II, CD80 + and CD86 + populations on the CD19 + cells. We compared B lymphocytes from cancer patients (cancer) and controls (control), and within these two groups, sCD40L and IL-4 treated (+) and untreated cells (-). (A) Expression of MHC-II shown as median of fluorescence intensity (MFI); 100% of the cells were positive for MHC-II expression. (B) Percentage of the CD80 + CD86 + population. Bars indicate groups with significant different results, based on ANOVA two-way test, where p

    Journal: PLoS ONE

    Article Title: B lymphocytes can be activated to act as antigen presenting cells to promote anti-tumor responses

    doi: 10.1371/journal.pone.0199034

    Figure Lengend Snippet: B lymphocytes from cancer patients can be activated by treatment with sCD40L and IL-4. Viable 90% enriched B lymphocytes from PBMCs from cervical cancer patients (cancer) or controls (control), were incubated with 10 μg/ml sCD40L and 50 ng/ml IL-4 for 24 hours. After harvesting, cells were labeled with anti-CD19, CD80, CD86 and HLA-DR and analyzed by flow cytometry, where 10 5 events were acquired in a FACSCanto (BD Biosciences, Carlsbad, CA). For data analyzes, we excluded debris and doublets and gated the MHC-II, CD80 + and CD86 + populations on the CD19 + cells. We compared B lymphocytes from cancer patients (cancer) and controls (control), and within these two groups, sCD40L and IL-4 treated (+) and untreated cells (-). (A) Expression of MHC-II shown as median of fluorescence intensity (MFI); 100% of the cells were positive for MHC-II expression. (B) Percentage of the CD80 + CD86 + population. Bars indicate groups with significant different results, based on ANOVA two-way test, where p

    Article Snippet: Cells were stimulated for 24 hours with sCD40L and IL-4 (Thermo Scientific, Waltham, MA, and Peprotech, Rocky Hill, NJ).

    Techniques: Incubation, Labeling, Flow Cytometry, Cytometry, Expressing, Fluorescence

    Serum inflammatory biomarkers levels in healthy subjects and across various rheumatic AF groups. AF: atrial fibrillation, hs‐CRP: high sensitivity C‐reactive protein, IL‐6: interleukin‐6, sCD‐40L: soluble CD‐40 Ligand. SI unit: mg (milligram); pg (picogram); ng (nanogram); l(liter); ml(milliliter)

    Journal: Journal of Arrhythmia

    Article Title: Role of inflammation in initiation and maintenance of atrial fibrillation in rheumatic mitral stenosis – An analytical cross‐sectional study, et al. Role of inflammation in initiation and maintenance of atrial fibrillation in rheumatic mitral stenosis – An analytical cross‐sectional study

    doi: 10.1002/joa3.12428

    Figure Lengend Snippet: Serum inflammatory biomarkers levels in healthy subjects and across various rheumatic AF groups. AF: atrial fibrillation, hs‐CRP: high sensitivity C‐reactive protein, IL‐6: interleukin‐6, sCD‐40L: soluble CD‐40 Ligand. SI unit: mg (milligram); pg (picogram); ng (nanogram); l(liter); ml(milliliter)

    Article Snippet: 2.4 Measurements of inflammatory biomarkers Biomarkers of inflammation—that is, high sensitivity C‐reactive protein (hs‐CRP), Interleukin‐6 (IL‐6) and soluble CD‐40 Ligand (sCD‐40L) were measured by standard ELISA immunoassay kits for hs‐CRP (BioCheck, Inc Foster City, USA ), IL‐6 (Quantikine Elisa, R & D Systems, Minneapolis, USA ), and sCD‐40L (Diaclone SAS, Besancon Cedex, France).

    Techniques:

    Flow diagram showing screening and recruitment of the study population. AF = atrial fibrillation, NSR = normal sinus rhythm, SCAF = subclinical transient AF, RHD = rheumatic heart disease, hs‐CRP = high sensitivity C‐reactive protein, IL‐6 = interlukin‐6, sCD‐40L = soluble CD‐40 Ligand

    Journal: Journal of Arrhythmia

    Article Title: Role of inflammation in initiation and maintenance of atrial fibrillation in rheumatic mitral stenosis – An analytical cross‐sectional study, et al. Role of inflammation in initiation and maintenance of atrial fibrillation in rheumatic mitral stenosis – An analytical cross‐sectional study

    doi: 10.1002/joa3.12428

    Figure Lengend Snippet: Flow diagram showing screening and recruitment of the study population. AF = atrial fibrillation, NSR = normal sinus rhythm, SCAF = subclinical transient AF, RHD = rheumatic heart disease, hs‐CRP = high sensitivity C‐reactive protein, IL‐6 = interlukin‐6, sCD‐40L = soluble CD‐40 Ligand

    Article Snippet: 2.4 Measurements of inflammatory biomarkers Biomarkers of inflammation—that is, high sensitivity C‐reactive protein (hs‐CRP), Interleukin‐6 (IL‐6) and soluble CD‐40 Ligand (sCD‐40L) were measured by standard ELISA immunoassay kits for hs‐CRP (BioCheck, Inc Foster City, USA ), IL‐6 (Quantikine Elisa, R & D Systems, Minneapolis, USA ), and sCD‐40L (Diaclone SAS, Besancon Cedex, France).

    Techniques:

    Diagram showing probable pathophysiologic mechanisms for initiation and maintenance of atrial fibrillation in rheumatic mitral stenosis. SCAF: subclinical transient atrial fibrillation, AF: atrial fibrillation, hs‐CRP: high sensitivity C‐reactive protein, IL‐6: interleukin‐6, sCD‐40L: soluble CD‐40 Ligand

    Journal: Journal of Arrhythmia

    Article Title: Role of inflammation in initiation and maintenance of atrial fibrillation in rheumatic mitral stenosis – An analytical cross‐sectional study, et al. Role of inflammation in initiation and maintenance of atrial fibrillation in rheumatic mitral stenosis – An analytical cross‐sectional study

    doi: 10.1002/joa3.12428

    Figure Lengend Snippet: Diagram showing probable pathophysiologic mechanisms for initiation and maintenance of atrial fibrillation in rheumatic mitral stenosis. SCAF: subclinical transient atrial fibrillation, AF: atrial fibrillation, hs‐CRP: high sensitivity C‐reactive protein, IL‐6: interleukin‐6, sCD‐40L: soluble CD‐40 Ligand

    Article Snippet: 2.4 Measurements of inflammatory biomarkers Biomarkers of inflammation—that is, high sensitivity C‐reactive protein (hs‐CRP), Interleukin‐6 (IL‐6) and soluble CD‐40 Ligand (sCD‐40L) were measured by standard ELISA immunoassay kits for hs‐CRP (BioCheck, Inc Foster City, USA ), IL‐6 (Quantikine Elisa, R & D Systems, Minneapolis, USA ), and sCD‐40L (Diaclone SAS, Besancon Cedex, France).

    Techniques:

    HIV-1 Tat induces platelet activation in vivo , which is abolished in the presence of VPA (A) The plasma concentration of sCD40L in C57BL/6 mice was measured via ELISA. Upon intravenous injection with HIV-1 Tat, there is a significant increase in the level of sCD40L found in the plasma; however, this is inhibited, to levels that mimic those of mice receiving saline, when VPA is administered once a day for three days prior to injection of Tat. (B) Platelet activation was verified in the samples described in panel A by ELISA specific for mouse platelet factor 4 (PF4). N=4 for each treatment group, labeled as intraperitoneal pretreatment/intravenous treatment. The concentrations of sCD40L (pg/mL) and PF4 (ng/mL) are presented as mean (± SEM) of three replicates for each sample. Statistical analysis was performed by one-way ANOVA followed by Bonferroni's test for multiple comparisons; *denotes p

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Anti-platelet activity of valproic acid contributes to decreased soluble CD40L production in human immunodeficiency virus type 1-infected individuals

    doi: 10.4049/jimmunol.1001911

    Figure Lengend Snippet: HIV-1 Tat induces platelet activation in vivo , which is abolished in the presence of VPA (A) The plasma concentration of sCD40L in C57BL/6 mice was measured via ELISA. Upon intravenous injection with HIV-1 Tat, there is a significant increase in the level of sCD40L found in the plasma; however, this is inhibited, to levels that mimic those of mice receiving saline, when VPA is administered once a day for three days prior to injection of Tat. (B) Platelet activation was verified in the samples described in panel A by ELISA specific for mouse platelet factor 4 (PF4). N=4 for each treatment group, labeled as intraperitoneal pretreatment/intravenous treatment. The concentrations of sCD40L (pg/mL) and PF4 (ng/mL) are presented as mean (± SEM) of three replicates for each sample. Statistical analysis was performed by one-way ANOVA followed by Bonferroni's test for multiple comparisons; *denotes p

    Article Snippet: Soluble CD40L or platelet factor 4 (PF4) concentrations were measured in PPP samples using a mouse sCD40L ELISA kit (Bender Med Systems, San Diego, CA) or PF4 ELISA kit (R & D Systems, Minneapolis, MN).

    Techniques: Activation Assay, In Vivo, Concentration Assay, Mouse Assay, Enzyme-linked Immunosorbent Assay, Injection, Labeling

    Cytoskeletal rearrangement is necessary for sCD40L release from platelets, and is attenuated following treatment with VPA (A) Supernatants from platelets pretreated with cytochalasin E were collected and ELISA analysis was performed. Cytoskeletal disruption with cytochalasin E reduced sCD40L release induced by the potent platelet activator thrombin. (B) Platelet spreading on fibrinogen-coated coverslips is attenuated following treatment with VPA, or another well-characterized GSK3β inhibitor LiCl. Differential interference contrast (DIC; top panels) and scanning electron microscopy (SEM; bottom panels) show increasing numbers of platelets that are partially spread with visible filopodia (`➢'), but not lamellipodia (*). Phalloidin staining reveals changes in patterns of F-actin fibers (Phal; middle panels). (C) Quantification of whole images represented in panel B; *** and ### denotes p

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Anti-platelet activity of valproic acid contributes to decreased soluble CD40L production in human immunodeficiency virus type 1-infected individuals

    doi: 10.4049/jimmunol.1001911

    Figure Lengend Snippet: Cytoskeletal rearrangement is necessary for sCD40L release from platelets, and is attenuated following treatment with VPA (A) Supernatants from platelets pretreated with cytochalasin E were collected and ELISA analysis was performed. Cytoskeletal disruption with cytochalasin E reduced sCD40L release induced by the potent platelet activator thrombin. (B) Platelet spreading on fibrinogen-coated coverslips is attenuated following treatment with VPA, or another well-characterized GSK3β inhibitor LiCl. Differential interference contrast (DIC; top panels) and scanning electron microscopy (SEM; bottom panels) show increasing numbers of platelets that are partially spread with visible filopodia (`➢'), but not lamellipodia (*). Phalloidin staining reveals changes in patterns of F-actin fibers (Phal; middle panels). (C) Quantification of whole images represented in panel B; *** and ### denotes p

    Article Snippet: Soluble CD40L or platelet factor 4 (PF4) concentrations were measured in PPP samples using a mouse sCD40L ELISA kit (Bender Med Systems, San Diego, CA) or PF4 ELISA kit (R & D Systems, Minneapolis, MN).

    Techniques: Enzyme-linked Immunosorbent Assay, Electron Microscopy, Staining

    VPA treatment significantly reduces circulating levels of sCD40L in HIV-infected patients Plasma samples were collected from HIV-infected individuals (n=23) before and after administration of VPA (500 mg/day) for 7 days and levels of soluble CD40L were measured via specific ELISA. The concentrations of sCD40L (pg/mL) are presented as mean (± SEM) of three replicates for each sample. Statistical significance was determined by paired t-test; ***denotes p

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Anti-platelet activity of valproic acid contributes to decreased soluble CD40L production in human immunodeficiency virus type 1-infected individuals

    doi: 10.4049/jimmunol.1001911

    Figure Lengend Snippet: VPA treatment significantly reduces circulating levels of sCD40L in HIV-infected patients Plasma samples were collected from HIV-infected individuals (n=23) before and after administration of VPA (500 mg/day) for 7 days and levels of soluble CD40L were measured via specific ELISA. The concentrations of sCD40L (pg/mL) are presented as mean (± SEM) of three replicates for each sample. Statistical significance was determined by paired t-test; ***denotes p

    Article Snippet: Soluble CD40L or platelet factor 4 (PF4) concentrations were measured in PPP samples using a mouse sCD40L ELISA kit (Bender Med Systems, San Diego, CA) or PF4 ELISA kit (R & D Systems, Minneapolis, MN).

    Techniques: Infection, Enzyme-linked Immunosorbent Assay

    VPA inhibits platelet release of sCD40L Release of sCD40L from purified human platelets is inhibited by VPA treatment, indicating that VPA affects platelet-derived plasma sCD40L levels. Purified platelets were treated as indicated and supernatants were collected for use in an ELISA specific for human sCD40L. Activators alone induced a significant amount of sCD40L release, which was inhibited following VPA treatment. sCD40L concentration (pg/mL) is presented as mean (± SEM) of three replicates for each sample from one donor representative of multiple donors. *denotes p

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Anti-platelet activity of valproic acid contributes to decreased soluble CD40L production in human immunodeficiency virus type 1-infected individuals

    doi: 10.4049/jimmunol.1001911

    Figure Lengend Snippet: VPA inhibits platelet release of sCD40L Release of sCD40L from purified human platelets is inhibited by VPA treatment, indicating that VPA affects platelet-derived plasma sCD40L levels. Purified platelets were treated as indicated and supernatants were collected for use in an ELISA specific for human sCD40L. Activators alone induced a significant amount of sCD40L release, which was inhibited following VPA treatment. sCD40L concentration (pg/mL) is presented as mean (± SEM) of three replicates for each sample from one donor representative of multiple donors. *denotes p

    Article Snippet: Soluble CD40L or platelet factor 4 (PF4) concentrations were measured in PPP samples using a mouse sCD40L ELISA kit (Bender Med Systems, San Diego, CA) or PF4 ELISA kit (R & D Systems, Minneapolis, MN).

    Techniques: Purification, Derivative Assay, Enzyme-linked Immunosorbent Assay, Concentration Assay

    sCD40L induced signaling is not significantly affected by cryopreservation. B cells from the same two healthy donors were analyzed before (fresh) and after (cryo) cryopreservation. The cells were stimulated with sCD40L (400 ng/mL) for 5 and 10 min before fixation and phospho flow analysis as described in Methods. Results are shown as mean ± standard deviation. Statistical significance was calculated between the two groups (fresh, cryo) by an ordinary two-way ANOVA with Sidak’s multiple comparisons test. ** p

    Journal: Scientific Reports

    Article Title: Cryopreservation of primary B cells minimally influences their signaling responses

    doi: 10.1038/s41598-018-36121-9

    Figure Lengend Snippet: sCD40L induced signaling is not significantly affected by cryopreservation. B cells from the same two healthy donors were analyzed before (fresh) and after (cryo) cryopreservation. The cells were stimulated with sCD40L (400 ng/mL) for 5 and 10 min before fixation and phospho flow analysis as described in Methods. Results are shown as mean ± standard deviation. Statistical significance was calculated between the two groups (fresh, cryo) by an ordinary two-way ANOVA with Sidak’s multiple comparisons test. ** p

    Article Snippet: Reagents and antibodies sCD40L (cat. no. 11343345) was from ImmunoTools, Germany and F(ab’)2 anti-human IgM (cat. no. 2022-01) was from Southern Biotechnology, CA, USA.

    Techniques: Flow Cytometry, Standard Deviation

    Association between UFP and sCD40L, platelets and leukocytes, 6-hour periods and lag 0 (0–23 hours) . sCD40L: soluble CD40Ligand. UFP: ultrafine particles (number concentration of particles with a size range of 0.01 to 0.1 μm).

    Journal: Particle and Fibre Toxicology

    Article Title: Ultrafine particles and platelet activation in patients with coronary heart disease - results from a prospective panel study

    doi: 10.1186/1743-8977-4-1

    Figure Lengend Snippet: Association between UFP and sCD40L, platelets and leukocytes, 6-hour periods and lag 0 (0–23 hours) . sCD40L: soluble CD40Ligand. UFP: ultrafine particles (number concentration of particles with a size range of 0.01 to 0.1 μm).

    Article Snippet: Recombinant human sCD40L standards (Bender Medsystems, Vienna, Austria) were added to each plate using serial dilutions in blocking buffer to create a standard curve of 6.25 to 400 pg/ml.

    Techniques: Concentration Assay

    Schematic representation of the hypothesised mechanisms involved in direct vascular effects of particles or their translocated components . * see Reference [12]. sCD40L: soluble CD40Ligand. vWF: von Willebrand factor.

    Journal: Particle and Fibre Toxicology

    Article Title: Ultrafine particles and platelet activation in patients with coronary heart disease - results from a prospective panel study

    doi: 10.1186/1743-8977-4-1

    Figure Lengend Snippet: Schematic representation of the hypothesised mechanisms involved in direct vascular effects of particles or their translocated components . * see Reference [12]. sCD40L: soluble CD40Ligand. vWF: von Willebrand factor.

    Article Snippet: Recombinant human sCD40L standards (Bender Medsystems, Vienna, Austria) were added to each plate using serial dilutions in blocking buffer to create a standard curve of 6.25 to 400 pg/ml.

    Techniques:

    Effects of IFN-γ and sCD40L on the CD40 induction in intestinal epithelial cells. Expression levels of CD40 mRNA were determined in HT29 cells and results presented as relation to the metastatic lymph node gene (MLN) 51 mRNA expression. Data are

    Journal: The American Journal of Pathology

    Article Title: The CD40-CD40L Pathway Contributes to the Proinflammatory Function of Intestinal Epithelial Cells in Inflammatory Bowel Disease

    doi: 10.2353/ajpath.2010.090461

    Figure Lengend Snippet: Effects of IFN-γ and sCD40L on the CD40 induction in intestinal epithelial cells. Expression levels of CD40 mRNA were determined in HT29 cells and results presented as relation to the metastatic lymph node gene (MLN) 51 mRNA expression. Data are

    Article Snippet: For analysis of the sCD40L stimulation IFN-γ–activated HT29 cells were grown to confluence. sCD40L was applied as human recombinant sCD40L in a trimeric 16.3 kDa form (Bender MedSystems, Vienna, Austria) and exposed to the basolateral compartment of the transwell system for 72 hours (0.1 to 100 ng/ml).

    Techniques: Expressing

    Effect of soluble CD40 ligand (sCD40L) on the cytokine profile. Peripheral blood mononuclear cells (PBMC) of HIV-infected patients (pat.) and control (ctrl.) donors were exposed to low-dose sCD40L (50 pg/ml) for 48 hours and subsequently stimulated with the CpG-P oligodeoxynucleotide 21798 for 20 hours. The levels of interleukin (IL-)2, interferon gamma (IFN-g), tumor necrosis factor alpha (TNF-a), IL-6, IL-8, and IL-10 were measured using a fluorescent bead assay. IL-4, IL-5, IL-12, TNF-beta, and IL-1 beta were not detected. Data are presented as mean and standard error. *p

    Journal: PLoS ONE

    Article Title: Chronic Immune Activation in HIV-1 Infection Contributes to Reduced Interferon Alpha Production via Enhanced CD40:CD40 Ligand Interaction

    doi: 10.1371/journal.pone.0033925

    Figure Lengend Snippet: Effect of soluble CD40 ligand (sCD40L) on the cytokine profile. Peripheral blood mononuclear cells (PBMC) of HIV-infected patients (pat.) and control (ctrl.) donors were exposed to low-dose sCD40L (50 pg/ml) for 48 hours and subsequently stimulated with the CpG-P oligodeoxynucleotide 21798 for 20 hours. The levels of interleukin (IL-)2, interferon gamma (IFN-g), tumor necrosis factor alpha (TNF-a), IL-6, IL-8, and IL-10 were measured using a fluorescent bead assay. IL-4, IL-5, IL-12, TNF-beta, and IL-1 beta were not detected. Data are presented as mean and standard error. *p

    Article Snippet: IL-3 and sCD40L concentrations were determined using the Quantikine Immunoassay human IL-3 (R & D Systems) and the human sCD40L module set (Bender Medsystems), respectively.

    Techniques: Infection, Fluorescent Bead Assay

    Suppression of interferon (IFN) alpha production by CD40 ligand. ( a ) Preincubation of peripheral blood mononuclear cells (PBMC) of controls without (w/o) or with (w/t) a cell-culture grade commercial soluble CD40 ligand (sCD40L; 3 µg/ml) for 48 hours (h), followed by stimulation with CpG-P 21798. Data represent 11 and 5 separate experiments for IL-3 levels of 0 and 5–5,000 pg/ml, respectively. The arrow indicates physiological IL-3 levels. ( b ) Neutralization of sCD40L with a cell-culture grade anti-CD40L antibody with subsequent CpG-P stimulation in two donors. Similar data were obtained using CpG-A (data not shown). ( c ) Direct effect of sCD40L (3 µg/ml) on the CpG-P induced IFN-alpha production by plasmacytoid dendritic cells (PDC) of control donors. ( d ) Expression of cell-associated CD40L (cCD40L) on baby hamster kidney (BHK) cells. ( e ) Shedding of sCD40L into the cell culture supernatants after 24 h and 48 h of culture. ( f ) Western Blot of concentrated supernatants of CD40L-expressing BHK cells and a commercial sCD40L preparation (R D Systems) using reducing conditions. Data represent three separate experiments. No bands were detected in the supernatants of BHK wild type cells (data not shown). ( g ) Inhibition of CpG-P induced IFN-alpha production using sCD40L from R D Systems and BHK cells. Statistics were calculated between PBMC of HIV-1 infected patients (pat.) (n = 5) and controls (ctrl.) (n = 10) exposed to sCD40L (R D Systems). ( h ) cCD40L expression on CD4+ and CD8+ T cells of control donors after stimulation with mock (grey filled curve) or PMA/ionomycin (black dotted curve). ( i ) Inhibition of CpG-P induced IFN-alpha production by increasing counts of untreated (unstim.) or PMA/ionomycin-stimulated (stim.) CD4+ T cells, which were left unfixed or fixed used 4% paraformaldehyde, and coincubated with 1×10 6 PBMC of five control donors. Similar results were obtained with CpG-A (data not shown). ( j ) Inhibition of CpG-P induced IFN-alpha production by paraformaldehyde-fixed cCD40L-expressing and wild type (WT) BHK cells using PBMC of eight control donors. Data are presented as mean and standard error. *p

    Journal: PLoS ONE

    Article Title: Chronic Immune Activation in HIV-1 Infection Contributes to Reduced Interferon Alpha Production via Enhanced CD40:CD40 Ligand Interaction

    doi: 10.1371/journal.pone.0033925

    Figure Lengend Snippet: Suppression of interferon (IFN) alpha production by CD40 ligand. ( a ) Preincubation of peripheral blood mononuclear cells (PBMC) of controls without (w/o) or with (w/t) a cell-culture grade commercial soluble CD40 ligand (sCD40L; 3 µg/ml) for 48 hours (h), followed by stimulation with CpG-P 21798. Data represent 11 and 5 separate experiments for IL-3 levels of 0 and 5–5,000 pg/ml, respectively. The arrow indicates physiological IL-3 levels. ( b ) Neutralization of sCD40L with a cell-culture grade anti-CD40L antibody with subsequent CpG-P stimulation in two donors. Similar data were obtained using CpG-A (data not shown). ( c ) Direct effect of sCD40L (3 µg/ml) on the CpG-P induced IFN-alpha production by plasmacytoid dendritic cells (PDC) of control donors. ( d ) Expression of cell-associated CD40L (cCD40L) on baby hamster kidney (BHK) cells. ( e ) Shedding of sCD40L into the cell culture supernatants after 24 h and 48 h of culture. ( f ) Western Blot of concentrated supernatants of CD40L-expressing BHK cells and a commercial sCD40L preparation (R D Systems) using reducing conditions. Data represent three separate experiments. No bands were detected in the supernatants of BHK wild type cells (data not shown). ( g ) Inhibition of CpG-P induced IFN-alpha production using sCD40L from R D Systems and BHK cells. Statistics were calculated between PBMC of HIV-1 infected patients (pat.) (n = 5) and controls (ctrl.) (n = 10) exposed to sCD40L (R D Systems). ( h ) cCD40L expression on CD4+ and CD8+ T cells of control donors after stimulation with mock (grey filled curve) or PMA/ionomycin (black dotted curve). ( i ) Inhibition of CpG-P induced IFN-alpha production by increasing counts of untreated (unstim.) or PMA/ionomycin-stimulated (stim.) CD4+ T cells, which were left unfixed or fixed used 4% paraformaldehyde, and coincubated with 1×10 6 PBMC of five control donors. Similar results were obtained with CpG-A (data not shown). ( j ) Inhibition of CpG-P induced IFN-alpha production by paraformaldehyde-fixed cCD40L-expressing and wild type (WT) BHK cells using PBMC of eight control donors. Data are presented as mean and standard error. *p

    Article Snippet: IL-3 and sCD40L concentrations were determined using the Quantikine Immunoassay human IL-3 (R & D Systems) and the human sCD40L module set (Bender Medsystems), respectively.

    Techniques: Cell Culture, Neutralization, Expressing, Western Blot, Inhibition, Infection