Journal: Journal of Virology

Article Title: Role of Regulatory Elements and the MAPK/ERK or p38 MAPK Pathways for Activation of Human Cytomegalovirus Gene Expression

doi: 10.1128/JVI.76.10.4873-4885.2002

Figure Lengend Snippet: Effect of p38 MAPK inhibitor SB202190 (FHPI) on CAT expression from the UL4-CAT promoter in recombinant virus-infected HFFs. Confluent HFFs were serum starved for 24 h and then treated with FHPI for 1 h before infection with various recombinant viruses in the presence of FHPI in quadruplicate as described in Materials and Methods. Cells were harvested at 14 h after infection and subjected to CAT assays. The results of the CAT assays were averaged, and the standard deviations were calculated. The CAT activity per microgram of protein at various concentrations of the drug is relative to that of the nontreated recombinant viruses.

Article Snippet: For infection of HFFs in the presence of the MEK inhibitor UO126 (Promega) or the p38 MAPK inhibitor SB202190 (FHPI) (Calbiochem Corp., San Diego, Calif.), the drugs were dissolved in dimethyl sulfoxide (DMSO) to make a 10 mM stock as recommended by the manufacturer.

Techniques: Expressing, Recombinant, Infection, Activity Assay