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    p38 MAP kinase inhibitor
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    99
    Millipore sb202190
    CD14-dependent activation of <t>p38</t> is required for IL-10 production. (A) Equal proteins from lysates of CD14 +/+ and CD14 −/− B6 BMDMs incubated with B. burgdorferi for 30 min were separated by 10% SDS-PAGE, electrotransferred to a nitrocellulose membrane, and probed with phospho-p38 and β-actin antibodies. CD14 +/+ BMDMs (B) or PMA-treated THP-1 cells (C) were treated with DMSO or increasing concentrations of <t>SB202190</t> for 30 min prior to incubation with B. burgdorferi . Cell culture supernatants were collected 24 h postinfection, and TNF and IL-10 levels were measured by ELISA. (D) CD14 +/+ BMDMs were pretreated with combinations of DMSO, 5 μM SB202190, and 10, 50, or 100 ng/ml of recombinant IL-10 and then coincubated with B. burgdorferi ( B.b .) at an MOI of 10. TNF was measured in the culture supernatants collected after 24 h. (E) B. burgdorferi organisms were coincubated with PBMCs from 10 patients with Lyme arthritis, 5 with the TLR1-1805GG SNP and 5 with the TLR1-1805TG/TT SNP, for 30 min. Cell lysates were analyzed for phospho-p38 by the bead-based Luminex assay (Millipore). For panel A, data are representative of results from two independent experiments. For panels B and C, data are representative of results from five independent experiments. For panels D and E, results represent means ± SEMs from two independent experiments. ** , P
    Sb202190, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sb202190/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sb202190 - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

    95
    Tocris sb202190
    Inhibition of GSK3 promotes activation of the MKK3/6-p38 MAPK-ATF2 signaling module. ( a ) Immunoblot analysis for activating phosphorylations of proteins of the MKK3/6-p38 MAPK-ATF2 signaling module in primary brown adipocytes pre-treated with 10 μM SB216763 for 1 h before stimulation with 0.1 μM ISO for 1 h. ( b ) Immunoblot analysis of total and phosphorylated p38 MAPK in immortalized brown adipocytes overexpressing GSK3β mutants or empty vector. Cells were treated with 0.1 μM ISO for 1 h. ( c ) Fgf21 expression in primary brown adipocytes pre-treated with 10 μM <t>SB202190</t> (p38 MAPK inhibitor) for 1 h before treatment with 10 μM SB216763 (GSK3 inhibitor) for 1 h, followed by stimulation with 0.1 μM ISO for additional 6 h. ( d ) Schematic presentation of the proposed mechanism through which GSK3 regulates the thermogenic gene program in brown adipocytes. Data presented as mean of means +SEM (n = 3). Statistical significance was determined by two-way ANOVA with repeated measures and Tukey’s post hoc test for multiple comparisons. *p
    Sb202190, supplied by Tocris, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sb202190/product/Tocris
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sb202190 - by Bioz Stars, 2021-04
    95/100 stars
      Buy from Supplier

    86
    Millipore sb 202190
    Inhibition of GSK3 promotes activation of the MKK3/6-p38 MAPK-ATF2 signaling module. ( a ) Immunoblot analysis for activating phosphorylations of proteins of the MKK3/6-p38 MAPK-ATF2 signaling module in primary brown adipocytes pre-treated with 10 μM SB216763 for 1 h before stimulation with 0.1 μM ISO for 1 h. ( b ) Immunoblot analysis of total and phosphorylated p38 MAPK in immortalized brown adipocytes overexpressing GSK3β mutants or empty vector. Cells were treated with 0.1 μM ISO for 1 h. ( c ) Fgf21 expression in primary brown adipocytes pre-treated with 10 μM <t>SB202190</t> (p38 MAPK inhibitor) for 1 h before treatment with 10 μM SB216763 (GSK3 inhibitor) for 1 h, followed by stimulation with 0.1 μM ISO for additional 6 h. ( d ) Schematic presentation of the proposed mechanism through which GSK3 regulates the thermogenic gene program in brown adipocytes. Data presented as mean of means +SEM (n = 3). Statistical significance was determined by two-way ANOVA with repeated measures and Tukey’s post hoc test for multiple comparisons. *p
    Sb 202190, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sb 202190/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sb 202190 - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    Image Search Results


    CD14-dependent activation of p38 is required for IL-10 production. (A) Equal proteins from lysates of CD14 +/+ and CD14 −/− B6 BMDMs incubated with B. burgdorferi for 30 min were separated by 10% SDS-PAGE, electrotransferred to a nitrocellulose membrane, and probed with phospho-p38 and β-actin antibodies. CD14 +/+ BMDMs (B) or PMA-treated THP-1 cells (C) were treated with DMSO or increasing concentrations of SB202190 for 30 min prior to incubation with B. burgdorferi . Cell culture supernatants were collected 24 h postinfection, and TNF and IL-10 levels were measured by ELISA. (D) CD14 +/+ BMDMs were pretreated with combinations of DMSO, 5 μM SB202190, and 10, 50, or 100 ng/ml of recombinant IL-10 and then coincubated with B. burgdorferi ( B.b .) at an MOI of 10. TNF was measured in the culture supernatants collected after 24 h. (E) B. burgdorferi organisms were coincubated with PBMCs from 10 patients with Lyme arthritis, 5 with the TLR1-1805GG SNP and 5 with the TLR1-1805TG/TT SNP, for 30 min. Cell lysates were analyzed for phospho-p38 by the bead-based Luminex assay (Millipore). For panel A, data are representative of results from two independent experiments. For panels B and C, data are representative of results from five independent experiments. For panels D and E, results represent means ± SEMs from two independent experiments. ** , P

    Journal: Infection and Immunity

    Article Title: Induction of Interleukin 10 by Borrelia burgdorferi Is Regulated by the Action of CD14-Dependent p38 Mitogen-Activated Protein Kinase and cAMP-Mediated Chromatin Remodeling

    doi: 10.1128/IAI.00781-17

    Figure Lengend Snippet: CD14-dependent activation of p38 is required for IL-10 production. (A) Equal proteins from lysates of CD14 +/+ and CD14 −/− B6 BMDMs incubated with B. burgdorferi for 30 min were separated by 10% SDS-PAGE, electrotransferred to a nitrocellulose membrane, and probed with phospho-p38 and β-actin antibodies. CD14 +/+ BMDMs (B) or PMA-treated THP-1 cells (C) were treated with DMSO or increasing concentrations of SB202190 for 30 min prior to incubation with B. burgdorferi . Cell culture supernatants were collected 24 h postinfection, and TNF and IL-10 levels were measured by ELISA. (D) CD14 +/+ BMDMs were pretreated with combinations of DMSO, 5 μM SB202190, and 10, 50, or 100 ng/ml of recombinant IL-10 and then coincubated with B. burgdorferi ( B.b .) at an MOI of 10. TNF was measured in the culture supernatants collected after 24 h. (E) B. burgdorferi organisms were coincubated with PBMCs from 10 patients with Lyme arthritis, 5 with the TLR1-1805GG SNP and 5 with the TLR1-1805TG/TT SNP, for 30 min. Cell lysates were analyzed for phospho-p38 by the bead-based Luminex assay (Millipore). For panel A, data are representative of results from two independent experiments. For panels B and C, data are representative of results from five independent experiments. For panels D and E, results represent means ± SEMs from two independent experiments. ** , P

    Article Snippet: BMDMs were treated with the p38 inhibitor SB202190 (each at 5 μM) (Sigma-Aldrich, St. Louis, MO) for 30 min prior to addition of B. burgdorferi at an MOI of 10 as reported earlier ( ).

    Techniques: Activation Assay, Incubation, SDS Page, Cell Culture, Enzyme-linked Immunosorbent Assay, Recombinant, Luminex

    p38 regulates binding of transcription factors STAT-3 and SP-1 to the il-10 promoter in B6 BMDMs. BMDMs were coincubated with B. burgdorferi at an MOI of 10 for 30 min in the presence or absence of 5 μM SB202190. Subsequently, a ChIP assay was performed to evaluate the binding of STAT3 to the il10 promoter at the −700 site (A) or −100 site (B). The data were normalized to the input DNA and are shown as fold enrichment over uninfected controls. (C) A similar strategy was used to evaluate the binding of SP1 to the i10 promoter. Results represent means ± SEMs from two independent experiments. The dotted line represents a 1.5-fold change, considered a significant change from the control. ** , P

    Journal: Infection and Immunity

    Article Title: Induction of Interleukin 10 by Borrelia burgdorferi Is Regulated by the Action of CD14-Dependent p38 Mitogen-Activated Protein Kinase and cAMP-Mediated Chromatin Remodeling

    doi: 10.1128/IAI.00781-17

    Figure Lengend Snippet: p38 regulates binding of transcription factors STAT-3 and SP-1 to the il-10 promoter in B6 BMDMs. BMDMs were coincubated with B. burgdorferi at an MOI of 10 for 30 min in the presence or absence of 5 μM SB202190. Subsequently, a ChIP assay was performed to evaluate the binding of STAT3 to the il10 promoter at the −700 site (A) or −100 site (B). The data were normalized to the input DNA and are shown as fold enrichment over uninfected controls. (C) A similar strategy was used to evaluate the binding of SP1 to the i10 promoter. Results represent means ± SEMs from two independent experiments. The dotted line represents a 1.5-fold change, considered a significant change from the control. ** , P

    Article Snippet: BMDMs were treated with the p38 inhibitor SB202190 (each at 5 μM) (Sigma-Aldrich, St. Louis, MO) for 30 min prior to addition of B. burgdorferi at an MOI of 10 as reported earlier ( ).

    Techniques: Binding Assay, Chromatin Immunoprecipitation

    Inhibition of GSK3 promotes activation of the MKK3/6-p38 MAPK-ATF2 signaling module. ( a ) Immunoblot analysis for activating phosphorylations of proteins of the MKK3/6-p38 MAPK-ATF2 signaling module in primary brown adipocytes pre-treated with 10 μM SB216763 for 1 h before stimulation with 0.1 μM ISO for 1 h. ( b ) Immunoblot analysis of total and phosphorylated p38 MAPK in immortalized brown adipocytes overexpressing GSK3β mutants or empty vector. Cells were treated with 0.1 μM ISO for 1 h. ( c ) Fgf21 expression in primary brown adipocytes pre-treated with 10 μM SB202190 (p38 MAPK inhibitor) for 1 h before treatment with 10 μM SB216763 (GSK3 inhibitor) for 1 h, followed by stimulation with 0.1 μM ISO for additional 6 h. ( d ) Schematic presentation of the proposed mechanism through which GSK3 regulates the thermogenic gene program in brown adipocytes. Data presented as mean of means +SEM (n = 3). Statistical significance was determined by two-way ANOVA with repeated measures and Tukey’s post hoc test for multiple comparisons. *p

    Journal: Scientific Reports

    Article Title: GSK3 is a negative regulator of the thermogenic program in brown adipocytes

    doi: 10.1038/s41598-018-21795-y

    Figure Lengend Snippet: Inhibition of GSK3 promotes activation of the MKK3/6-p38 MAPK-ATF2 signaling module. ( a ) Immunoblot analysis for activating phosphorylations of proteins of the MKK3/6-p38 MAPK-ATF2 signaling module in primary brown adipocytes pre-treated with 10 μM SB216763 for 1 h before stimulation with 0.1 μM ISO for 1 h. ( b ) Immunoblot analysis of total and phosphorylated p38 MAPK in immortalized brown adipocytes overexpressing GSK3β mutants or empty vector. Cells were treated with 0.1 μM ISO for 1 h. ( c ) Fgf21 expression in primary brown adipocytes pre-treated with 10 μM SB202190 (p38 MAPK inhibitor) for 1 h before treatment with 10 μM SB216763 (GSK3 inhibitor) for 1 h, followed by stimulation with 0.1 μM ISO for additional 6 h. ( d ) Schematic presentation of the proposed mechanism through which GSK3 regulates the thermogenic gene program in brown adipocytes. Data presented as mean of means +SEM (n = 3). Statistical significance was determined by two-way ANOVA with repeated measures and Tukey’s post hoc test for multiple comparisons. *p

    Article Snippet: SB415286, SB216763, BIO, H89 and SB202190 were obtained from Tocris Bioscience.

    Techniques: Inhibition, Activation Assay, Plasmid Preparation, Expressing