sars cov 2 nucleocapsid his recombinant protein Search Results


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    Sino Biological sars cov 2 2019 ncov nucleocapsid his recombinant protein covid 19 nucleocapsid research
    Sars Cov 2 2019 Ncov Nucleocapsid His Recombinant Protein Covid 19 Nucleocapsid Research, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Sino Biological sars cov 2
    Sars Cov 2, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sars cov 2/product/Sino Biological
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    93
    Rockland Immunochemicals anti sars nucleoprotein
    Anti Sars Nucleoprotein, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti sars nucleoprotein/product/Rockland Immunochemicals
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    94
    Native Antigen Inc recombinant sars cov 2 nucleocapsid phosphoprotein
    Cellular immune responses to <t>SARS-CoV-2.</t> a , b IFNγ SFU measured in PBMCs and stimulated with spike protein peptide pools (PP) peptide in ( a ) rhesus and ( b ) cynomolgus macaques. PBMC samples were isolated from uninfected animals (naïve) or at early (days 4 and 5) and late (days 14-19) time-points following SARS-CoV-2 infection. Box plots show the group median + /− inter-quartile range, with minimum and maximum values connected by whiskers. Two-tailed Mann–Whitney U-test carried out to compare pre and post-SARS-Cov2 infection where * p ≤ 0.05, ** p ≤ 0.01. c , d IFNγ SFU measured in PBMC in response to spike protein megapools (MP) in ( c ) rhesus and ( d ) cynomolgus macaques or, ( e ) in mononuclear cells isolated from lung and spleen. Bars show the group median with SFU measured in individual animals shown as dots. Rhesus macaques summed MP naïve vs late time point p = 0.01. Cynomolgus macaques naïve vs PP9 p = 0.03, naïve vs MP2 p = 0.03, naïve vs MP3 p = 0.01, naïve vs summed p = 0.01. Biologically independent animal samples for ( a – d ); PBMCs: naïve n = 6, early n = 2, late n = 4 Biologically independent animal samples for ( e ); lung and spleen: early n = 2, late n = 4, ( f – j ) Frequency of major lymphocyte and monocyte cell populations quantified by immunophenotyping assay ( f – h ) CD4+, CD8+ and γδ T-cell frequencies in PBMCs and lung cells, ( i ) Monocyte subtype frequency in PBMCs and lung MNCs, ( j ) Natural killer (NK) cell subset frequency in PBMCs and lung MNCs. Stacked bars show the group median with 95% confidence intervals. Biologically independent animal samples for ( f – j ); PBMC: Naïve rhesus n = 8, early rhesus n = 1, late rhesus n = 2, naïve cyno = 7, early cyno n = 2, late cyno n = 2. Lung: early rhesus n = 2, late rhesus n = 3, early cyno n = 2, late cyno n = 2. k – n Intracellular cytokine staining data. k – l Cytokine and activation marker detection in CD4+, CD8+and γδ T-cells in PBMCs stimulated with M, N and S peptide pools. m – n CD107a expression in CD8+ and γδ T-cells in PBMCs. Bars show the group median with cell frequencies measured in individual animals shown as dots.
    Recombinant Sars Cov 2 Nucleocapsid Phosphoprotein, supplied by Native Antigen Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant sars cov 2 nucleocapsid phosphoprotein/product/Native Antigen Inc
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    N/A
    Coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch Nucleocapsid protein is a most abundant protein of coronavirus
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    N/A
    Source E coli Recombinant 2019 nCoV Nucleocapsid Protein is produced by E coli expression system The target protein is expressed with sequence Met1 Ala419 of 2019 ncov Nucleocapsid fused with
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    Image Search Results


    Cellular immune responses to SARS-CoV-2. a , b IFNγ SFU measured in PBMCs and stimulated with spike protein peptide pools (PP) peptide in ( a ) rhesus and ( b ) cynomolgus macaques. PBMC samples were isolated from uninfected animals (naïve) or at early (days 4 and 5) and late (days 14-19) time-points following SARS-CoV-2 infection. Box plots show the group median + /− inter-quartile range, with minimum and maximum values connected by whiskers. Two-tailed Mann–Whitney U-test carried out to compare pre and post-SARS-Cov2 infection where * p ≤ 0.05, ** p ≤ 0.01. c , d IFNγ SFU measured in PBMC in response to spike protein megapools (MP) in ( c ) rhesus and ( d ) cynomolgus macaques or, ( e ) in mononuclear cells isolated from lung and spleen. Bars show the group median with SFU measured in individual animals shown as dots. Rhesus macaques summed MP naïve vs late time point p = 0.01. Cynomolgus macaques naïve vs PP9 p = 0.03, naïve vs MP2 p = 0.03, naïve vs MP3 p = 0.01, naïve vs summed p = 0.01. Biologically independent animal samples for ( a – d ); PBMCs: naïve n = 6, early n = 2, late n = 4 Biologically independent animal samples for ( e ); lung and spleen: early n = 2, late n = 4, ( f – j ) Frequency of major lymphocyte and monocyte cell populations quantified by immunophenotyping assay ( f – h ) CD4+, CD8+ and γδ T-cell frequencies in PBMCs and lung cells, ( i ) Monocyte subtype frequency in PBMCs and lung MNCs, ( j ) Natural killer (NK) cell subset frequency in PBMCs and lung MNCs. Stacked bars show the group median with 95% confidence intervals. Biologically independent animal samples for ( f – j ); PBMC: Naïve rhesus n = 8, early rhesus n = 1, late rhesus n = 2, naïve cyno = 7, early cyno n = 2, late cyno n = 2. Lung: early rhesus n = 2, late rhesus n = 3, early cyno n = 2, late cyno n = 2. k – n Intracellular cytokine staining data. k – l Cytokine and activation marker detection in CD4+, CD8+and γδ T-cells in PBMCs stimulated with M, N and S peptide pools. m – n CD107a expression in CD8+ and γδ T-cells in PBMCs. Bars show the group median with cell frequencies measured in individual animals shown as dots.

    Journal: Nature Communications

    Article Title: Comparison of rhesus and cynomolgus macaques as an infection model for COVID-19

    doi: 10.1038/s41467-021-21389-9

    Figure Lengend Snippet: Cellular immune responses to SARS-CoV-2. a , b IFNγ SFU measured in PBMCs and stimulated with spike protein peptide pools (PP) peptide in ( a ) rhesus and ( b ) cynomolgus macaques. PBMC samples were isolated from uninfected animals (naïve) or at early (days 4 and 5) and late (days 14-19) time-points following SARS-CoV-2 infection. Box plots show the group median + /− inter-quartile range, with minimum and maximum values connected by whiskers. Two-tailed Mann–Whitney U-test carried out to compare pre and post-SARS-Cov2 infection where * p ≤ 0.05, ** p ≤ 0.01. c , d IFNγ SFU measured in PBMC in response to spike protein megapools (MP) in ( c ) rhesus and ( d ) cynomolgus macaques or, ( e ) in mononuclear cells isolated from lung and spleen. Bars show the group median with SFU measured in individual animals shown as dots. Rhesus macaques summed MP naïve vs late time point p = 0.01. Cynomolgus macaques naïve vs PP9 p = 0.03, naïve vs MP2 p = 0.03, naïve vs MP3 p = 0.01, naïve vs summed p = 0.01. Biologically independent animal samples for ( a – d ); PBMCs: naïve n = 6, early n = 2, late n = 4 Biologically independent animal samples for ( e ); lung and spleen: early n = 2, late n = 4, ( f – j ) Frequency of major lymphocyte and monocyte cell populations quantified by immunophenotyping assay ( f – h ) CD4+, CD8+ and γδ T-cell frequencies in PBMCs and lung cells, ( i ) Monocyte subtype frequency in PBMCs and lung MNCs, ( j ) Natural killer (NK) cell subset frequency in PBMCs and lung MNCs. Stacked bars show the group median with 95% confidence intervals. Biologically independent animal samples for ( f – j ); PBMC: Naïve rhesus n = 8, early rhesus n = 1, late rhesus n = 2, naïve cyno = 7, early cyno n = 2, late cyno n = 2. Lung: early rhesus n = 2, late rhesus n = 3, early cyno n = 2, late cyno n = 2. k – n Intracellular cytokine staining data. k – l Cytokine and activation marker detection in CD4+, CD8+and γδ T-cells in PBMCs stimulated with M, N and S peptide pools. m – n CD107a expression in CD8+ and γδ T-cells in PBMCs. Bars show the group median with cell frequencies measured in individual animals shown as dots.

    Article Snippet: Recombinant SARS-CoV-2 Nucleocapsid phosphoprotein (GenBank: MN908947, isolate Wuhan-Hu-1) was expressed and purified from Escherichia coli as full-length nucleoprotein (amino acids 1-419) with a C-terminal 6xHis-Tag (REC31812-100, Batch #20042310, Native Antigen Company).

    Techniques: Isolation, Infection, Two Tailed Test, MANN-WHITNEY, Staining, Activation Assay, Marker, Expressing

    SARS-CoV-2-specific IgG antibodies measured by ELISA in naïve and SARS-CoV-2 infected macaques. Spike- ( a ), Receptor-Binding Domain- ( b ) and Nucleoprotein- ( c ) specific IgG antibodies measured in sera of rhesus and cynomolgus macaques. Sera were collected from uninfected animals (day 0) or 1–3, 4–6, 8–9, 11–12 and 14–19 days following SARS-CoV-2 infection. n = 6 at 0, 1–3 and 4–6 dpc; n = 4 at 8–9, 11–12 and 14 dpc. Bars show the group mean±SEM with an endpoint titre determined for each individual animal shown as squares for males and dots for females. * p ≤ 0.05 (Kruskal–Wallis one-way ANOVA, two-sided). Experiment performed in duplicates.

    Journal: Nature Communications

    Article Title: Comparison of rhesus and cynomolgus macaques as an infection model for COVID-19

    doi: 10.1038/s41467-021-21389-9

    Figure Lengend Snippet: SARS-CoV-2-specific IgG antibodies measured by ELISA in naïve and SARS-CoV-2 infected macaques. Spike- ( a ), Receptor-Binding Domain- ( b ) and Nucleoprotein- ( c ) specific IgG antibodies measured in sera of rhesus and cynomolgus macaques. Sera were collected from uninfected animals (day 0) or 1–3, 4–6, 8–9, 11–12 and 14–19 days following SARS-CoV-2 infection. n = 6 at 0, 1–3 and 4–6 dpc; n = 4 at 8–9, 11–12 and 14 dpc. Bars show the group mean±SEM with an endpoint titre determined for each individual animal shown as squares for males and dots for females. * p ≤ 0.05 (Kruskal–Wallis one-way ANOVA, two-sided). Experiment performed in duplicates.

    Article Snippet: Recombinant SARS-CoV-2 Nucleocapsid phosphoprotein (GenBank: MN908947, isolate Wuhan-Hu-1) was expressed and purified from Escherichia coli as full-length nucleoprotein (amino acids 1-419) with a C-terminal 6xHis-Tag (REC31812-100, Batch #20042310, Native Antigen Company).

    Techniques: Enzyme-linked Immunosorbent Assay, Infection, Binding Assay

    Presence of viral RNA and IL-6 by ISH in cynomolgus and rhesus macaques during SARS-CoV-2 infection. ISH detection of abundant viral RNA (RNAScope, red chromogen) within the areas of pneumonia ( a , arrows) and occasionally in the BALT ( a , insert, arrow) in cynomolgus ( a , b ) and rhesus macaques ( b , arrows) at 4/5 dpc. Small amount of viral RNA in the interalveolar septa from cynomolgus ( c , arrows) and rhesus macaques ( d , arrows) at 14/15 dpc. Image analysis of positively stained area in RNASCope labelled sections for viral RNA ( e , whole slide); n = 2 macaques per species and time point; bars represent median values. Abundant presence of IL-6 mRNA in the areas of pneumonia from cynomolgus ( f , arrows) and rhesus macaques ( g , arrows) at 4/5 dpc. Small amount of IL-6 mRNA positive cells within the interalveolar septa from cynomolgus ( h , arrows) and rhesus macaques ( i , arrows) at 14/15 dpc. Image analysis of positively stained area in RNASCope labelled sections for IL-6 mRNA ( j , areas of lesion); n = 2 macaques per species and time point; bars represent median values. Bars in micrographs=200 µm.

    Journal: Nature Communications

    Article Title: Comparison of rhesus and cynomolgus macaques as an infection model for COVID-19

    doi: 10.1038/s41467-021-21389-9

    Figure Lengend Snippet: Presence of viral RNA and IL-6 by ISH in cynomolgus and rhesus macaques during SARS-CoV-2 infection. ISH detection of abundant viral RNA (RNAScope, red chromogen) within the areas of pneumonia ( a , arrows) and occasionally in the BALT ( a , insert, arrow) in cynomolgus ( a , b ) and rhesus macaques ( b , arrows) at 4/5 dpc. Small amount of viral RNA in the interalveolar septa from cynomolgus ( c , arrows) and rhesus macaques ( d , arrows) at 14/15 dpc. Image analysis of positively stained area in RNASCope labelled sections for viral RNA ( e , whole slide); n = 2 macaques per species and time point; bars represent median values. Abundant presence of IL-6 mRNA in the areas of pneumonia from cynomolgus ( f , arrows) and rhesus macaques ( g , arrows) at 4/5 dpc. Small amount of IL-6 mRNA positive cells within the interalveolar septa from cynomolgus ( h , arrows) and rhesus macaques ( i , arrows) at 14/15 dpc. Image analysis of positively stained area in RNASCope labelled sections for IL-6 mRNA ( j , areas of lesion); n = 2 macaques per species and time point; bars represent median values. Bars in micrographs=200 µm.

    Article Snippet: Recombinant SARS-CoV-2 Nucleocapsid phosphoprotein (GenBank: MN908947, isolate Wuhan-Hu-1) was expressed and purified from Escherichia coli as full-length nucleoprotein (amino acids 1-419) with a C-terminal 6xHis-Tag (REC31812-100, Batch #20042310, Native Antigen Company).

    Techniques: In Situ Hybridization, Infection, Staining

    CTscan images from cynomolgus and rhesus macaques infected with SARS-CoV-2 and culled at 18 days post challenge. Images constructed from CT scans collected 18 days after challenge with SARS-CoV-2 showing pulmonary abnormalities in two cynomolgus ( a , b ) and one rhesus macaque ( c ). Arrows in images ( a1 ), ( b1 ) and ( c1 ) indicate areas of peripheral ground glass opacification. Arrows in images ( a2 ) and ( c2 ) indicate areas of ground glass opacification and arrow in image ( b2 ) indicates an area of consolidation. Images from a second rhesus macaque did not have abnormal features.

    Journal: Nature Communications

    Article Title: Comparison of rhesus and cynomolgus macaques as an infection model for COVID-19

    doi: 10.1038/s41467-021-21389-9

    Figure Lengend Snippet: CTscan images from cynomolgus and rhesus macaques infected with SARS-CoV-2 and culled at 18 days post challenge. Images constructed from CT scans collected 18 days after challenge with SARS-CoV-2 showing pulmonary abnormalities in two cynomolgus ( a , b ) and one rhesus macaque ( c ). Arrows in images ( a1 ), ( b1 ) and ( c1 ) indicate areas of peripheral ground glass opacification. Arrows in images ( a2 ) and ( c2 ) indicate areas of ground glass opacification and arrow in image ( b2 ) indicates an area of consolidation. Images from a second rhesus macaque did not have abnormal features.

    Article Snippet: Recombinant SARS-CoV-2 Nucleocapsid phosphoprotein (GenBank: MN908947, isolate Wuhan-Hu-1) was expressed and purified from Escherichia coli as full-length nucleoprotein (amino acids 1-419) with a C-terminal 6xHis-Tag (REC31812-100, Batch #20042310, Native Antigen Company).

    Techniques: Infection, Construct

    Histopathological changes in cynomolgus and rhesus macaques during SARS-CoV-2 infection. Areas of alveolar necrosis observed in cynomolgus macaques at 4/5 dpc with shrunken, eosinophilic cells within the alveolar walls ( a , b ), together with alveolar oedema ( a , arrows; bar = 100 µm), type II pneumocyte hyperplasia and expanded alveolar spaces with inflammatory cell infiltration ( b , arrows; bar = 100 µm). Occasional multinucleated cells are observed ( b , insert; bar = 20 µm). Similar histopathological changes observed in rhesus macaques, including alveolar necrosis and areas with patchy alveolar oedema ( c , arrow; bar = 100 µm), and accumulatios of alveolar macrophages ( d , arrow; bar = 100 µm) and bronchial exudates ( d , insert; bar = 50 µm). Histopathological changes with less severity observed at 14/15 dpc in cynomolgus macaques, with infiltration of mononuclear cells within alveolar spaces and bronchiolar lumen ( e , arrows; bar = 100 µm) and perivascular cuffing ( f , arrow; bar = 100 µm). Bronchiole regeneration ( g , arrow; bar = 100 µm) and perivascular/peribronchiolar cuffing observed in rhesus macaques at 14/15 dpc ( h , arrows; bar = 100 µm), together with BALT proliferation ( h , *; bar = 100 µm). Representative images from 6 slides per animal at each time point.

    Journal: Nature Communications

    Article Title: Comparison of rhesus and cynomolgus macaques as an infection model for COVID-19

    doi: 10.1038/s41467-021-21389-9

    Figure Lengend Snippet: Histopathological changes in cynomolgus and rhesus macaques during SARS-CoV-2 infection. Areas of alveolar necrosis observed in cynomolgus macaques at 4/5 dpc with shrunken, eosinophilic cells within the alveolar walls ( a , b ), together with alveolar oedema ( a , arrows; bar = 100 µm), type II pneumocyte hyperplasia and expanded alveolar spaces with inflammatory cell infiltration ( b , arrows; bar = 100 µm). Occasional multinucleated cells are observed ( b , insert; bar = 20 µm). Similar histopathological changes observed in rhesus macaques, including alveolar necrosis and areas with patchy alveolar oedema ( c , arrow; bar = 100 µm), and accumulatios of alveolar macrophages ( d , arrow; bar = 100 µm) and bronchial exudates ( d , insert; bar = 50 µm). Histopathological changes with less severity observed at 14/15 dpc in cynomolgus macaques, with infiltration of mononuclear cells within alveolar spaces and bronchiolar lumen ( e , arrows; bar = 100 µm) and perivascular cuffing ( f , arrow; bar = 100 µm). Bronchiole regeneration ( g , arrow; bar = 100 µm) and perivascular/peribronchiolar cuffing observed in rhesus macaques at 14/15 dpc ( h , arrows; bar = 100 µm), together with BALT proliferation ( h , *; bar = 100 µm). Representative images from 6 slides per animal at each time point.

    Article Snippet: Recombinant SARS-CoV-2 Nucleocapsid phosphoprotein (GenBank: MN908947, isolate Wuhan-Hu-1) was expressed and purified from Escherichia coli as full-length nucleoprotein (amino acids 1-419) with a C-terminal 6xHis-Tag (REC31812-100, Batch #20042310, Native Antigen Company).

    Techniques: Infection