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  • 99
    Millipore salmonella enterica serovar typhimurium tv119 ra mutant lipid a
    Salmonella Enterica Serovar Typhimurium Tv119 Ra Mutant Lipid A, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 7 article reviews
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    salmonella enterica serovar typhimurium tv119 ra mutant lipid a - by Bioz Stars, 2020-09
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    95
    Millipore salmonella lps strains
    Assembly of the export apparatus results in pronounced membrane remodeling. ( A ) A vertical section from in situ cryo-ET structure of the injectisome from a ΔinvA S . <t>Typhimurium</t> strain (the dotted box denotes area of detail shown in B through G ). ( B-G ) Vertical sections of a cryo-EM ( B - D ) and cryo-ET ( E - G ) structures of the inner rings of the needle complex T3SS complex overlaid with a section ( B and E ), surface rendering ( C and F ) or hydrophobicity surface representation ( D and G ) of the FliP/FliQ/FliR (homologs of SpaP/SpaQ/SpaR from S . Typhimurium) isolated complex (PDB 6F2E). Red and blue colors ( D and G ) on the surface representation of the FliP/FliQ/FliR isolated complex represent hydrophobic and charge residues, respectively.
    Salmonella Lps Strains, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/salmonella lps strains/product/Millipore
    Average 95 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    salmonella lps strains - by Bioz Stars, 2020-09
    95/100 stars
      Buy from Supplier

    Image Search Results


    Assembly of the export apparatus results in pronounced membrane remodeling. ( A ) A vertical section from in situ cryo-ET structure of the injectisome from a ΔinvA S . Typhimurium strain (the dotted box denotes area of detail shown in B through G ). ( B-G ) Vertical sections of a cryo-EM ( B - D ) and cryo-ET ( E - G ) structures of the inner rings of the needle complex T3SS complex overlaid with a section ( B and E ), surface rendering ( C and F ) or hydrophobicity surface representation ( D and G ) of the FliP/FliQ/FliR (homologs of SpaP/SpaQ/SpaR from S . Typhimurium) isolated complex (PDB 6F2E). Red and blue colors ( D and G ) on the surface representation of the FliP/FliQ/FliR isolated complex represent hydrophobic and charge residues, respectively.

    Journal: bioRxiv

    Article Title: High-resolution view of the type III secretion export apparatus in situ reveals membrane remodeling and a secretion pathway

    doi: 10.1101/709592

    Figure Lengend Snippet: Assembly of the export apparatus results in pronounced membrane remodeling. ( A ) A vertical section from in situ cryo-ET structure of the injectisome from a ΔinvA S . Typhimurium strain (the dotted box denotes area of detail shown in B through G ). ( B-G ) Vertical sections of a cryo-EM ( B - D ) and cryo-ET ( E - G ) structures of the inner rings of the needle complex T3SS complex overlaid with a section ( B and E ), surface rendering ( C and F ) or hydrophobicity surface representation ( D and G ) of the FliP/FliQ/FliR (homologs of SpaP/SpaQ/SpaR from S . Typhimurium) isolated complex (PDB 6F2E). Red and blue colors ( D and G ) on the surface representation of the FliP/FliQ/FliR isolated complex represent hydrophobic and charge residues, respectively.

    Article Snippet: Typhimurium strains as indicated above.

    Techniques: In Situ, Isolation

    Interaction of the periplasmic PrgK ring with the export apparatus is required for type III secretion function but not for injectisome assembly. (A - F) Top view ( A ) and cross section ( D ) of the 3D reconstruction of the IR1 and IR2 rings of the needle complex containing the core components of the export apparatus. The interface between PrgK and the export apparatus is highlighted (in blue and red rectangles). The specific densities corresponding to PrgK residues 93-96 targeted for mutagenesis and functional analysis are shown in more detailed in the zoom-in views depicted in panels B through F . ( G ) Representative class averages of needle complexes isolated from S. Typhimurium expressing wild type PrgK or the indicated deletion mutants. ( H ) Type III protein secretion analysis of S. Typhimurium strains expressing the PrgK mutants with the indicated deletions in the region that interacts with the core components of the export apparatus. Whole cell lysates and cultured supernatants of the indicated strains were analyzed by Western immunoblot for the presence of SipB, SipC, and InvJ, which are substrates of the S. Typhimurium type III secretion system.

    Journal: bioRxiv

    Article Title: High-resolution view of the type III secretion export apparatus in situ reveals membrane remodeling and a secretion pathway

    doi: 10.1101/709592

    Figure Lengend Snippet: Interaction of the periplasmic PrgK ring with the export apparatus is required for type III secretion function but not for injectisome assembly. (A - F) Top view ( A ) and cross section ( D ) of the 3D reconstruction of the IR1 and IR2 rings of the needle complex containing the core components of the export apparatus. The interface between PrgK and the export apparatus is highlighted (in blue and red rectangles). The specific densities corresponding to PrgK residues 93-96 targeted for mutagenesis and functional analysis are shown in more detailed in the zoom-in views depicted in panels B through F . ( G ) Representative class averages of needle complexes isolated from S. Typhimurium expressing wild type PrgK or the indicated deletion mutants. ( H ) Type III protein secretion analysis of S. Typhimurium strains expressing the PrgK mutants with the indicated deletions in the region that interacts with the core components of the export apparatus. Whole cell lysates and cultured supernatants of the indicated strains were analyzed by Western immunoblot for the presence of SipB, SipC, and InvJ, which are substrates of the S. Typhimurium type III secretion system.

    Article Snippet: Typhimurium strains as indicated above.

    Techniques: Mutagenesis, Functional Assay, Isolation, Expressing, Cell Culture, Western Blot

    Vertical sections of the cryo ET in situ structures of injectisomes obtained from S. Typhimurium ΔspaS (A), or from a strain expressing a mutant form of InvA that lacks its last 328 amino acids (B). Also shown is a horizontal section of the latter structure (C).

    Journal: bioRxiv

    Article Title: High-resolution view of the type III secretion export apparatus in situ reveals membrane remodeling and a secretion pathway

    doi: 10.1101/709592

    Figure Lengend Snippet: Vertical sections of the cryo ET in situ structures of injectisomes obtained from S. Typhimurium ΔspaS (A), or from a strain expressing a mutant form of InvA that lacks its last 328 amino acids (B). Also shown is a horizontal section of the latter structure (C).

    Article Snippet: Typhimurium strains as indicated above.

    Techniques: In Situ, Expressing, Mutagenesis

    Comparison of the cryo-EM structure of the isolated needle complex IR1 and IR2 containing the core components of the export apparatus (A) with the cryo ET structures of the type III secretion needle complex from a S . Typhimurium ΔinvA mutant strain (B). The overlaid structures are shown in (C).

    Journal: bioRxiv

    Article Title: High-resolution view of the type III secretion export apparatus in situ reveals membrane remodeling and a secretion pathway

    doi: 10.1101/709592

    Figure Lengend Snippet: Comparison of the cryo-EM structure of the isolated needle complex IR1 and IR2 containing the core components of the export apparatus (A) with the cryo ET structures of the type III secretion needle complex from a S . Typhimurium ΔinvA mutant strain (B). The overlaid structures are shown in (C).

    Article Snippet: Typhimurium strains as indicated above.

    Techniques: Isolation, Mutagenesis

    In situ structure of InvA, an essential component of the type III secretion export apparatus. (A) A vertical section from the in situ cryo-ET structure of the injectisome from wild-type S . Typhimurium (the dotted box denotes area of detail shown in B through G ). ( B - G ) Vertical ( B - D ) and horizontal (through the plane indicated in panel B) ( E - G ) sections highlighting the structure of InvA. Side ( C ) and cut-away (D) views of the InvA structure (in pink) are shown and the location of the small (AR1) and large (AR2) rings are denoted. The overlaid of the structure of the FliP/FliQ/FliR isolated complex is shown in yellow. ( F and G ) Bottom views of the InvA nonameric ring, with the overlaid atomic structure of the cytoplasmic domain of an InvA homolog (PDB-4A5P) ( G ).

    Journal: bioRxiv

    Article Title: High-resolution view of the type III secretion export apparatus in situ reveals membrane remodeling and a secretion pathway

    doi: 10.1101/709592

    Figure Lengend Snippet: In situ structure of InvA, an essential component of the type III secretion export apparatus. (A) A vertical section from the in situ cryo-ET structure of the injectisome from wild-type S . Typhimurium (the dotted box denotes area of detail shown in B through G ). ( B - G ) Vertical ( B - D ) and horizontal (through the plane indicated in panel B) ( E - G ) sections highlighting the structure of InvA. Side ( C ) and cut-away (D) views of the InvA structure (in pink) are shown and the location of the small (AR1) and large (AR2) rings are denoted. The overlaid of the structure of the FliP/FliQ/FliR isolated complex is shown in yellow. ( F and G ) Bottom views of the InvA nonameric ring, with the overlaid atomic structure of the cytoplasmic domain of an InvA homolog (PDB-4A5P) ( G ).

    Article Snippet: Typhimurium strains as indicated above.

    Techniques: In Situ, Isolation

    Cryo-EM and cryo-ET analyses of the core components the T3SS export apparatus. (A) A schematic representation of the T3SS injectisome. ( B and C ) Horizontal ( B ) and vertical ( C ) sections of a 3D reconstruction of a cryo-EM structure of the inner rings of the needle complex containing the export apparatus (denoted with a yellow arrow). ( D ) A vertical section from an in situ cryo-ET structure of the injectisome from a S . Typhimurium ΔinvA mutant strain. The export apparatus is denoted with a yellow arrow. ( E and F ) Horizontal ( E ) and vertical ( F ) sections of a 3D reconstruction of a cryo-EM structure of the inner rings of the needle complex obtained from a S. Typhimurium mutant strain (ΔspaP ΔspaQ ΔspaR ΔspaS ) lacking the core components of the export apparatus. ( G ) A vertical section from in situ cryo-ET structure of the injectisome from a ΔspaP ΔspaQ ΔspaR ΔspaS S . Typhimurium strain. The position where the export apparatus would be located is denoted with a black arrow. IR1: inner ring 1; IM: inner membrane.

    Journal: bioRxiv

    Article Title: High-resolution view of the type III secretion export apparatus in situ reveals membrane remodeling and a secretion pathway

    doi: 10.1101/709592

    Figure Lengend Snippet: Cryo-EM and cryo-ET analyses of the core components the T3SS export apparatus. (A) A schematic representation of the T3SS injectisome. ( B and C ) Horizontal ( B ) and vertical ( C ) sections of a 3D reconstruction of a cryo-EM structure of the inner rings of the needle complex containing the export apparatus (denoted with a yellow arrow). ( D ) A vertical section from an in situ cryo-ET structure of the injectisome from a S . Typhimurium ΔinvA mutant strain. The export apparatus is denoted with a yellow arrow. ( E and F ) Horizontal ( E ) and vertical ( F ) sections of a 3D reconstruction of a cryo-EM structure of the inner rings of the needle complex obtained from a S. Typhimurium mutant strain (ΔspaP ΔspaQ ΔspaR ΔspaS ) lacking the core components of the export apparatus. ( G ) A vertical section from in situ cryo-ET structure of the injectisome from a ΔspaP ΔspaQ ΔspaR ΔspaS S . Typhimurium strain. The position where the export apparatus would be located is denoted with a black arrow. IR1: inner ring 1; IM: inner membrane.

    Article Snippet: Typhimurium strains as indicated above.

    Techniques: In Situ, Mutagenesis

    Mutations in a periplasmic domain of the needle complex protein PrgK that interacts with the core components of the export apparatus do not affect needle complex base structure or export apparatus deployment. Shown are representative class averages of needle complexes isolated from S . Typhimurium expressing wild type PrgK or the indicated PrgK deletion mutants.

    Journal: bioRxiv

    Article Title: High-resolution view of the type III secretion export apparatus in situ reveals membrane remodeling and a secretion pathway

    doi: 10.1101/709592

    Figure Lengend Snippet: Mutations in a periplasmic domain of the needle complex protein PrgK that interacts with the core components of the export apparatus do not affect needle complex base structure or export apparatus deployment. Shown are representative class averages of needle complexes isolated from S . Typhimurium expressing wild type PrgK or the indicated PrgK deletion mutants.

    Article Snippet: Typhimurium strains as indicated above.

    Techniques: Isolation, Expressing

    Vertical sections of the cryo ET in situ structures of injectisomes obtained from wild type S. Typhimurium (A) or ΔspaS mutant strain (B), Also shown are a horizontal sections of the latter structure strain through the planes indicated in panel B (C and D). OM: bacterial outer membrane; IM: bacterial inner membrane; IR1 and IR2: inner rings 1 and 2 of the T3SS needle complex; PQR: SpaP/SpaQ/SpaR complex; AR1 and AR2: membrane proximal (AR1) and distal (AR2) cytoplasmic rings of InvA.

    Journal: bioRxiv

    Article Title: High-resolution view of the type III secretion export apparatus in situ reveals membrane remodeling and a secretion pathway

    doi: 10.1101/709592

    Figure Lengend Snippet: Vertical sections of the cryo ET in situ structures of injectisomes obtained from wild type S. Typhimurium (A) or ΔspaS mutant strain (B), Also shown are a horizontal sections of the latter structure strain through the planes indicated in panel B (C and D). OM: bacterial outer membrane; IM: bacterial inner membrane; IR1 and IR2: inner rings 1 and 2 of the T3SS needle complex; PQR: SpaP/SpaQ/SpaR complex; AR1 and AR2: membrane proximal (AR1) and distal (AR2) cytoplasmic rings of InvA.

    Article Snippet: Typhimurium strains as indicated above.

    Techniques: In Situ, Mutagenesis