saccharomyces cerevisiae Search Results


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  • 95
    ATCC s cerevisiae
    Study of toxicity Zn/CS NPs/Dox-AuMNPs. ( A ) Determination of hemolytic activity of prepared nanoparticles. The number of erythrocytes was 2.42 × 10 12 . Tested variants: water, CS (0.25 mg/mL), AuMNPs (1 mg/mL), Zn/CS NPs/Dox-AuMNPs (Dox = 6 µM) and AntiSar/Zn/CS NPs/Dox-AuMNPs (Dox = 6 µM). Inset photographs of each variant (centrifugation 2000 rpm, 5 min. to a completely clear solution). The pellet was diluted by PBS. The supernatant was spectrophotometrically analyzed, the absorbance spectra was obtained and the results were evaluated in 570 nm. Experiment was carried out using two replicates. ( B ) Toxicity evaluation of complexes using eukaryotic cells Saccharomyces <t>cerevisiae</t> . Characteristic growth curves for CS (0.25 mg/mL), AuMNPs (1 mg/mL), Zn/CS NPs/Dox-AuMNPs, AntiSar/Zn/CS NPs/Dox-AuMNPs and Dox (0.75 µM). ( C ) Average inhibition effect of samples: (0) S. cerevisiae , (1) AuMNPs (1 mg/mL), (2) CS (2 mg/mL), (3) Zn/CS NPs/Dox-AuMNPs (Dox 6 µM), (4) AntiSar/Zn/CS NPs/Dox-AuMNPs (Dox 6 µM), (5) Dox (0.25 µM), (6) Dox (0.5 µM), (7) Dox (1 µM). Data are expressed as an average of 12 repetitions. See to Materials and Methods Section for more information regarding experimental details.
    S Cerevisiae, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 416 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore saccharomyces cerevisiae
    (a) ALDH zymogram: 1–6, Saccharomyces <t>cerevisiae</t> ALDH controls: 1, 0.1 U; 2, 0.25 U; 3, 0.5 U; 4, 0.75 U; 5, 1 U; 6, 3 U; 7, blank; 8, S. gordonii V2016wt. (b) ADH zymogram: 1–3, S. gordonii V2016wt, V2016Δ adhE and V288; 4 and
    Saccharomyces Cerevisiae, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1266 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Roche saccharomyces cerevisiae
    ) from 13 sequences, including, in addition to the sequences shown, ALKBH8 from Caenorhabditis elegans and Drosophila melanogaster , ALKBH8 and KIAA1456 from Gallus gallus and Xenopus tropicalis , and Trm9 from Arabidopsis thaliana . Arrows indicate a GXGXG motif expected to be critical for methyltransferase activity. Hs, Homo sapiens ; Mm, Mus musculus ; Sc, Saccharomyces <t>cerevisiae</t> ; Sp, Schizosaccharomyces pombe .
    Saccharomyces Cerevisiae, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Millipore baker yeast β glucan
    Upon expression on surface of HEK293 cells α X β 2 supports adhesive, migratory and phagocytic activities of the cells toward C. albicans . ( A ) HEK293/α X β 2 cell adhesion to C. albicans . A total of 5 × 10 5 HEK293/α X β 2 (▪) or mock-transfected (□) cells were added to wells of tissue culture plates containing germinated (hyphae) or nongerminated (yeast) C. albicans of WT (SC5314) or ΔPra1 (CAMB5-18) strains. The plates were incubated at 37°C for 1 h, then nonadherent cells were removed by washing, and the adherent cells were quantified using the CyQUANT Cell Proliferation Assay kit. For inhibition assays, before addition to the plate wells, the cells were preincubated with 10 μg/ml of the Abs: anti-α M I-domain (44a), anti-α M lectin domain (OKM1), anti-β 2 (IB4), anti-α X (3.9), and irrelevant anti–MHC-II (W6/32) or with 1 mM <t>β-glucan,</t> 1 mM mannan, or 5 μM recombinant NIF for 10 min at room temperature. ( B ) HEK293/α X β 2 cell migration to C. albicans . Cell migration was measured in Boyden chambers (Costar Transwell with 8-μm porosity in a 24-well format). A total of 10 5 HEK293/α X β 2 cells were added to the upper chamber, whereas lower chambers contained 10 6 germinated C. albicans cells of WT SC5314 strain (□) or 10 mM vitronectin (▪) in serum-free DMEM/F-12 medium. The inhibitors mAbs IB4, OKM1, 3.9 (see above), anti-α V (27217E6), and 1 mM β-glucan or echistatin were added with the cells to the upper chamber. Plates were incubated for 8 h in a humidified incubator at 37°C and 5% CO 2 . The migrated cells were counted using the CyQUANT Cell Proliferation kit. ( C ) Phagocytosis of C. albicans by HEK293/α X β 2 cells. A total of 10 5 germinated C. albicans SC5314 strain cells were incubated with 3 × 10 5 (1:3 ratio), 7 × 10 5 (1:7 ratio), or 1.1 × 10 6 (1:11 ratio) mock-transfected HEK293 cells (●) or HEK293/α X β 2 cells in the presence (▴) or absence (○) of anti-α X mAbs 3.9. Fungal survival was determined every 20 min by plating the sample aliquots onto SDA plates in serial dilutions. Results are presented as percentage (mean ± SE) of total cells (to which was assigned the value of 100%) and represent the results of three independent experiments, each in triplicate. * p
    Baker Yeast β Glucan, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore baker s yeast β glucan standard
    Upon expression on surface of HEK293 cells α X β 2 supports adhesive, migratory and phagocytic activities of the cells toward C. albicans . ( A ) HEK293/α X β 2 cell adhesion to C. albicans . A total of 5 × 10 5 HEK293/α X β 2 (▪) or mock-transfected (□) cells were added to wells of tissue culture plates containing germinated (hyphae) or nongerminated (yeast) C. albicans of WT (SC5314) or ΔPra1 (CAMB5-18) strains. The plates were incubated at 37°C for 1 h, then nonadherent cells were removed by washing, and the adherent cells were quantified using the CyQUANT Cell Proliferation Assay kit. For inhibition assays, before addition to the plate wells, the cells were preincubated with 10 μg/ml of the Abs: anti-α M I-domain (44a), anti-α M lectin domain (OKM1), anti-β 2 (IB4), anti-α X (3.9), and irrelevant anti–MHC-II (W6/32) or with 1 mM <t>β-glucan,</t> 1 mM mannan, or 5 μM recombinant NIF for 10 min at room temperature. ( B ) HEK293/α X β 2 cell migration to C. albicans . Cell migration was measured in Boyden chambers (Costar Transwell with 8-μm porosity in a 24-well format). A total of 10 5 HEK293/α X β 2 cells were added to the upper chamber, whereas lower chambers contained 10 6 germinated C. albicans cells of WT SC5314 strain (□) or 10 mM vitronectin (▪) in serum-free DMEM/F-12 medium. The inhibitors mAbs IB4, OKM1, 3.9 (see above), anti-α V (27217E6), and 1 mM β-glucan or echistatin were added with the cells to the upper chamber. Plates were incubated for 8 h in a humidified incubator at 37°C and 5% CO 2 . The migrated cells were counted using the CyQUANT Cell Proliferation kit. ( C ) Phagocytosis of C. albicans by HEK293/α X β 2 cells. A total of 10 5 germinated C. albicans SC5314 strain cells were incubated with 3 × 10 5 (1:3 ratio), 7 × 10 5 (1:7 ratio), or 1.1 × 10 6 (1:11 ratio) mock-transfected HEK293 cells (●) or HEK293/α X β 2 cells in the presence (▴) or absence (○) of anti-α X mAbs 3.9. Fungal survival was determined every 20 min by plating the sample aliquots onto SDA plates in serial dilutions. Results are presented as percentage (mean ± SE) of total cells (to which was assigned the value of 100%) and represent the results of three independent experiments, each in triplicate. * p
    Baker S Yeast β Glucan Standard, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Fisher Scientific baker s yeast
    Upon expression on surface of HEK293 cells α X β 2 supports adhesive, migratory and phagocytic activities of the cells toward C. albicans . ( A ) HEK293/α X β 2 cell adhesion to C. albicans . A total of 5 × 10 5 HEK293/α X β 2 (▪) or mock-transfected (□) cells were added to wells of tissue culture plates containing germinated (hyphae) or nongerminated (yeast) C. albicans of WT (SC5314) or ΔPra1 (CAMB5-18) strains. The plates were incubated at 37°C for 1 h, then nonadherent cells were removed by washing, and the adherent cells were quantified using the CyQUANT Cell Proliferation Assay kit. For inhibition assays, before addition to the plate wells, the cells were preincubated with 10 μg/ml of the Abs: anti-α M I-domain (44a), anti-α M lectin domain (OKM1), anti-β 2 (IB4), anti-α X (3.9), and irrelevant anti–MHC-II (W6/32) or with 1 mM <t>β-glucan,</t> 1 mM mannan, or 5 μM recombinant NIF for 10 min at room temperature. ( B ) HEK293/α X β 2 cell migration to C. albicans . Cell migration was measured in Boyden chambers (Costar Transwell with 8-μm porosity in a 24-well format). A total of 10 5 HEK293/α X β 2 cells were added to the upper chamber, whereas lower chambers contained 10 6 germinated C. albicans cells of WT SC5314 strain (□) or 10 mM vitronectin (▪) in serum-free DMEM/F-12 medium. The inhibitors mAbs IB4, OKM1, 3.9 (see above), anti-α V (27217E6), and 1 mM β-glucan or echistatin were added with the cells to the upper chamber. Plates were incubated for 8 h in a humidified incubator at 37°C and 5% CO 2 . The migrated cells were counted using the CyQUANT Cell Proliferation kit. ( C ) Phagocytosis of C. albicans by HEK293/α X β 2 cells. A total of 10 5 germinated C. albicans SC5314 strain cells were incubated with 3 × 10 5 (1:3 ratio), 7 × 10 5 (1:7 ratio), or 1.1 × 10 6 (1:11 ratio) mock-transfected HEK293 cells (●) or HEK293/α X β 2 cells in the presence (▴) or absence (○) of anti-α X mAbs 3.9. Fungal survival was determined every 20 min by plating the sample aliquots onto SDA plates in serial dilutions. Results are presented as percentage (mean ± SE) of total cells (to which was assigned the value of 100%) and represent the results of three independent experiments, each in triplicate. * p
    Baker S Yeast, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Immuno Research Inc baker s yeast
    Upon expression on surface of HEK293 cells α X β 2 supports adhesive, migratory and phagocytic activities of the cells toward C. albicans . ( A ) HEK293/α X β 2 cell adhesion to C. albicans . A total of 5 × 10 5 HEK293/α X β 2 (▪) or mock-transfected (□) cells were added to wells of tissue culture plates containing germinated (hyphae) or nongerminated (yeast) C. albicans of WT (SC5314) or ΔPra1 (CAMB5-18) strains. The plates were incubated at 37°C for 1 h, then nonadherent cells were removed by washing, and the adherent cells were quantified using the CyQUANT Cell Proliferation Assay kit. For inhibition assays, before addition to the plate wells, the cells were preincubated with 10 μg/ml of the Abs: anti-α M I-domain (44a), anti-α M lectin domain (OKM1), anti-β 2 (IB4), anti-α X (3.9), and irrelevant anti–MHC-II (W6/32) or with 1 mM <t>β-glucan,</t> 1 mM mannan, or 5 μM recombinant NIF for 10 min at room temperature. ( B ) HEK293/α X β 2 cell migration to C. albicans . Cell migration was measured in Boyden chambers (Costar Transwell with 8-μm porosity in a 24-well format). A total of 10 5 HEK293/α X β 2 cells were added to the upper chamber, whereas lower chambers contained 10 6 germinated C. albicans cells of WT SC5314 strain (□) or 10 mM vitronectin (▪) in serum-free DMEM/F-12 medium. The inhibitors mAbs IB4, OKM1, 3.9 (see above), anti-α V (27217E6), and 1 mM β-glucan or echistatin were added with the cells to the upper chamber. Plates were incubated for 8 h in a humidified incubator at 37°C and 5% CO 2 . The migrated cells were counted using the CyQUANT Cell Proliferation kit. ( C ) Phagocytosis of C. albicans by HEK293/α X β 2 cells. A total of 10 5 germinated C. albicans SC5314 strain cells were incubated with 3 × 10 5 (1:3 ratio), 7 × 10 5 (1:7 ratio), or 1.1 × 10 6 (1:11 ratio) mock-transfected HEK293 cells (●) or HEK293/α X β 2 cells in the presence (▴) or absence (○) of anti-α X mAbs 3.9. Fungal survival was determined every 20 min by plating the sample aliquots onto SDA plates in serial dilutions. Results are presented as percentage (mean ± SE) of total cells (to which was assigned the value of 100%) and represent the results of three independent experiments, each in triplicate. * p
    Baker S Yeast, supplied by Immuno Research Inc, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore baker s yeast rna
    Upon expression on surface of HEK293 cells α X β 2 supports adhesive, migratory and phagocytic activities of the cells toward C. albicans . ( A ) HEK293/α X β 2 cell adhesion to C. albicans . A total of 5 × 10 5 HEK293/α X β 2 (▪) or mock-transfected (□) cells were added to wells of tissue culture plates containing germinated (hyphae) or nongerminated (yeast) C. albicans of WT (SC5314) or ΔPra1 (CAMB5-18) strains. The plates were incubated at 37°C for 1 h, then nonadherent cells were removed by washing, and the adherent cells were quantified using the CyQUANT Cell Proliferation Assay kit. For inhibition assays, before addition to the plate wells, the cells were preincubated with 10 μg/ml of the Abs: anti-α M I-domain (44a), anti-α M lectin domain (OKM1), anti-β 2 (IB4), anti-α X (3.9), and irrelevant anti–MHC-II (W6/32) or with 1 mM <t>β-glucan,</t> 1 mM mannan, or 5 μM recombinant NIF for 10 min at room temperature. ( B ) HEK293/α X β 2 cell migration to C. albicans . Cell migration was measured in Boyden chambers (Costar Transwell with 8-μm porosity in a 24-well format). A total of 10 5 HEK293/α X β 2 cells were added to the upper chamber, whereas lower chambers contained 10 6 germinated C. albicans cells of WT SC5314 strain (□) or 10 mM vitronectin (▪) in serum-free DMEM/F-12 medium. The inhibitors mAbs IB4, OKM1, 3.9 (see above), anti-α V (27217E6), and 1 mM β-glucan or echistatin were added with the cells to the upper chamber. Plates were incubated for 8 h in a humidified incubator at 37°C and 5% CO 2 . The migrated cells were counted using the CyQUANT Cell Proliferation kit. ( C ) Phagocytosis of C. albicans by HEK293/α X β 2 cells. A total of 10 5 germinated C. albicans SC5314 strain cells were incubated with 3 × 10 5 (1:3 ratio), 7 × 10 5 (1:7 ratio), or 1.1 × 10 6 (1:11 ratio) mock-transfected HEK293 cells (●) or HEK293/α X β 2 cells in the presence (▴) or absence (○) of anti-α X mAbs 3.9. Fungal survival was determined every 20 min by plating the sample aliquots onto SDA plates in serial dilutions. Results are presented as percentage (mean ± SE) of total cells (to which was assigned the value of 100%) and represent the results of three independent experiments, each in triplicate. * p
    Baker S Yeast Rna, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore baker s yeast gr
    Upon expression on surface of HEK293 cells α X β 2 supports adhesive, migratory and phagocytic activities of the cells toward C. albicans . ( A ) HEK293/α X β 2 cell adhesion to C. albicans . A total of 5 × 10 5 HEK293/α X β 2 (▪) or mock-transfected (□) cells were added to wells of tissue culture plates containing germinated (hyphae) or nongerminated (yeast) C. albicans of WT (SC5314) or ΔPra1 (CAMB5-18) strains. The plates were incubated at 37°C for 1 h, then nonadherent cells were removed by washing, and the adherent cells were quantified using the CyQUANT Cell Proliferation Assay kit. For inhibition assays, before addition to the plate wells, the cells were preincubated with 10 μg/ml of the Abs: anti-α M I-domain (44a), anti-α M lectin domain (OKM1), anti-β 2 (IB4), anti-α X (3.9), and irrelevant anti–MHC-II (W6/32) or with 1 mM <t>β-glucan,</t> 1 mM mannan, or 5 μM recombinant NIF for 10 min at room temperature. ( B ) HEK293/α X β 2 cell migration to C. albicans . Cell migration was measured in Boyden chambers (Costar Transwell with 8-μm porosity in a 24-well format). A total of 10 5 HEK293/α X β 2 cells were added to the upper chamber, whereas lower chambers contained 10 6 germinated C. albicans cells of WT SC5314 strain (□) or 10 mM vitronectin (▪) in serum-free DMEM/F-12 medium. The inhibitors mAbs IB4, OKM1, 3.9 (see above), anti-α V (27217E6), and 1 mM β-glucan or echistatin were added with the cells to the upper chamber. Plates were incubated for 8 h in a humidified incubator at 37°C and 5% CO 2 . The migrated cells were counted using the CyQUANT Cell Proliferation kit. ( C ) Phagocytosis of C. albicans by HEK293/α X β 2 cells. A total of 10 5 germinated C. albicans SC5314 strain cells were incubated with 3 × 10 5 (1:3 ratio), 7 × 10 5 (1:7 ratio), or 1.1 × 10 6 (1:11 ratio) mock-transfected HEK293 cells (●) or HEK293/α X β 2 cells in the presence (▴) or absence (○) of anti-α X mAbs 3.9. Fungal survival was determined every 20 min by plating the sample aliquots onto SDA plates in serial dilutions. Results are presented as percentage (mean ± SE) of total cells (to which was assigned the value of 100%) and represent the results of three independent experiments, each in triplicate. * p
    Baker S Yeast Gr, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore enolase from baker s yeast
    Upon expression on surface of HEK293 cells α X β 2 supports adhesive, migratory and phagocytic activities of the cells toward C. albicans . ( A ) HEK293/α X β 2 cell adhesion to C. albicans . A total of 5 × 10 5 HEK293/α X β 2 (▪) or mock-transfected (□) cells were added to wells of tissue culture plates containing germinated (hyphae) or nongerminated (yeast) C. albicans of WT (SC5314) or ΔPra1 (CAMB5-18) strains. The plates were incubated at 37°C for 1 h, then nonadherent cells were removed by washing, and the adherent cells were quantified using the CyQUANT Cell Proliferation Assay kit. For inhibition assays, before addition to the plate wells, the cells were preincubated with 10 μg/ml of the Abs: anti-α M I-domain (44a), anti-α M lectin domain (OKM1), anti-β 2 (IB4), anti-α X (3.9), and irrelevant anti–MHC-II (W6/32) or with 1 mM <t>β-glucan,</t> 1 mM mannan, or 5 μM recombinant NIF for 10 min at room temperature. ( B ) HEK293/α X β 2 cell migration to C. albicans . Cell migration was measured in Boyden chambers (Costar Transwell with 8-μm porosity in a 24-well format). A total of 10 5 HEK293/α X β 2 cells were added to the upper chamber, whereas lower chambers contained 10 6 germinated C. albicans cells of WT SC5314 strain (□) or 10 mM vitronectin (▪) in serum-free DMEM/F-12 medium. The inhibitors mAbs IB4, OKM1, 3.9 (see above), anti-α V (27217E6), and 1 mM β-glucan or echistatin were added with the cells to the upper chamber. Plates were incubated for 8 h in a humidified incubator at 37°C and 5% CO 2 . The migrated cells were counted using the CyQUANT Cell Proliferation kit. ( C ) Phagocytosis of C. albicans by HEK293/α X β 2 cells. A total of 10 5 germinated C. albicans SC5314 strain cells were incubated with 3 × 10 5 (1:3 ratio), 7 × 10 5 (1:7 ratio), or 1.1 × 10 6 (1:11 ratio) mock-transfected HEK293 cells (●) or HEK293/α X β 2 cells in the presence (▴) or absence (○) of anti-α X mAbs 3.9. Fungal survival was determined every 20 min by plating the sample aliquots onto SDA plates in serial dilutions. Results are presented as percentage (mean ± SE) of total cells (to which was assigned the value of 100%) and represent the results of three independent experiments, each in triplicate. * p
    Enolase From Baker S Yeast, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore s cerevisiae invertase
    Biosynthesis of precursor oligosaccharides for enzymatic remodeling. MALDI-TOF MS analysis (top panels) and 600-MHz 1 H NMR characterization (bottom panels) corresponding to: ( a ) Man 5 GlcNAc 2 glycan synthesized by enzymatic deglycosylation of S. <t>cerevisiae</t> oligosaccharides; and ( b ) the Man 3 GlcNAc 2 glycan synthesized by glycoengineered E. coli cells.
    S Cerevisiae Invertase, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore ribonucleic acid from baker s yeast
    Biosynthesis of precursor oligosaccharides for enzymatic remodeling. MALDI-TOF MS analysis (top panels) and 600-MHz 1 H NMR characterization (bottom panels) corresponding to: ( a ) Man 5 GlcNAc 2 glycan synthesized by enzymatic deglycosylation of S. <t>cerevisiae</t> oligosaccharides; and ( b ) the Man 3 GlcNAc 2 glycan synthesized by glycoengineered E. coli cells.
    Ribonucleic Acid From Baker S Yeast, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Difco baker s yeast saccharomyces cerevisiae
    Biosynthesis of precursor oligosaccharides for enzymatic remodeling. MALDI-TOF MS analysis (top panels) and 600-MHz 1 H NMR characterization (bottom panels) corresponding to: ( a ) Man 5 GlcNAc 2 glycan synthesized by enzymatic deglycosylation of S. <t>cerevisiae</t> oligosaccharides; and ( b ) the Man 3 GlcNAc 2 glycan synthesized by glycoengineered E. coli cells.
    Baker S Yeast Saccharomyces Cerevisiae, supplied by Difco, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Valiant brewer s yeast
    Biosynthesis of precursor oligosaccharides for enzymatic remodeling. MALDI-TOF MS analysis (top panels) and 600-MHz 1 H NMR characterization (bottom panels) corresponding to: ( a ) Man 5 GlcNAc 2 glycan synthesized by enzymatic deglycosylation of S. <t>cerevisiae</t> oligosaccharides; and ( b ) the Man 3 GlcNAc 2 glycan synthesized by glycoengineered E. coli cells.
    Brewer S Yeast, supplied by Valiant, used in various techniques. Bioz Stars score: 91/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore yeast rna
    Generality of ssRNA detection. Hairpin-guides were designed to target two additional physiologically relevant transcribed RNAs: (A) human homologue of murine double minute 2 (hDM2) and (B) human epidermal growth factor receptor 2 (HER2). The sequences to which complementary guides were designed are indicated with targeted positions shown in parentheses. The designed hairpin-guides were annealed to their intended targets, followed by incubation of 1 nM annealed target with the CLuciferase-E2C and Aart-NLuciferase biosensors. (C) The hDM2, HER2, and <t>VEGF</t> hairpin-guides were each annealed in the presence of 1 nM hDM2, HER2, and VEGF <t>RNA</t> targets, followed by incubation with CLuciferase-E2C and Aart-NLuciferase. ALU, arbitrary luminescent units.
    Yeast Rna, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 691 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Rockland Immunochemicals baker s yeast rna
    Generality of ssRNA detection. Hairpin-guides were designed to target two additional physiologically relevant transcribed RNAs: (A) human homologue of murine double minute 2 (hDM2) and (B) human epidermal growth factor receptor 2 (HER2). The sequences to which complementary guides were designed are indicated with targeted positions shown in parentheses. The designed hairpin-guides were annealed to their intended targets, followed by incubation of 1 nM annealed target with the CLuciferase-E2C and Aart-NLuciferase biosensors. (C) The hDM2, HER2, and <t>VEGF</t> hairpin-guides were each annealed in the presence of 1 nM hDM2, HER2, and VEGF <t>RNA</t> targets, followed by incubation with CLuciferase-E2C and Aart-NLuciferase. ALU, arbitrary luminescent units.
    Baker S Yeast Rna, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/baker s yeast rna/product/Rockland Immunochemicals
    Average 88 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    baker s yeast rna - by Bioz Stars, 2020-08
    88/100 stars
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    93
    Millipore baker s yeast g6pd
    Generality of ssRNA detection. Hairpin-guides were designed to target two additional physiologically relevant transcribed RNAs: (A) human homologue of murine double minute 2 (hDM2) and (B) human epidermal growth factor receptor 2 (HER2). The sequences to which complementary guides were designed are indicated with targeted positions shown in parentheses. The designed hairpin-guides were annealed to their intended targets, followed by incubation of 1 nM annealed target with the CLuciferase-E2C and Aart-NLuciferase biosensors. (C) The hDM2, HER2, and <t>VEGF</t> hairpin-guides were each annealed in the presence of 1 nM hDM2, HER2, and VEGF <t>RNA</t> targets, followed by incubation with CLuciferase-E2C and Aart-NLuciferase. ALU, arbitrary luminescent units.
    Baker S Yeast G6pd, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/baker s yeast g6pd/product/Millipore
    Average 93 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    baker s yeast g6pd - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    Image Search Results


    Study of toxicity Zn/CS NPs/Dox-AuMNPs. ( A ) Determination of hemolytic activity of prepared nanoparticles. The number of erythrocytes was 2.42 × 10 12 . Tested variants: water, CS (0.25 mg/mL), AuMNPs (1 mg/mL), Zn/CS NPs/Dox-AuMNPs (Dox = 6 µM) and AntiSar/Zn/CS NPs/Dox-AuMNPs (Dox = 6 µM). Inset photographs of each variant (centrifugation 2000 rpm, 5 min. to a completely clear solution). The pellet was diluted by PBS. The supernatant was spectrophotometrically analyzed, the absorbance spectra was obtained and the results were evaluated in 570 nm. Experiment was carried out using two replicates. ( B ) Toxicity evaluation of complexes using eukaryotic cells Saccharomyces cerevisiae . Characteristic growth curves for CS (0.25 mg/mL), AuMNPs (1 mg/mL), Zn/CS NPs/Dox-AuMNPs, AntiSar/Zn/CS NPs/Dox-AuMNPs and Dox (0.75 µM). ( C ) Average inhibition effect of samples: (0) S. cerevisiae , (1) AuMNPs (1 mg/mL), (2) CS (2 mg/mL), (3) Zn/CS NPs/Dox-AuMNPs (Dox 6 µM), (4) AntiSar/Zn/CS NPs/Dox-AuMNPs (Dox 6 µM), (5) Dox (0.25 µM), (6) Dox (0.5 µM), (7) Dox (1 µM). Data are expressed as an average of 12 repetitions. See to Materials and Methods Section for more information regarding experimental details.

    Journal: Nanomaterials

    Article Title: Zinc-Modified Nanotransporter of Doxorubicin for Targeted Prostate Cancer Delivery

    doi: 10.3390/nano7120435

    Figure Lengend Snippet: Study of toxicity Zn/CS NPs/Dox-AuMNPs. ( A ) Determination of hemolytic activity of prepared nanoparticles. The number of erythrocytes was 2.42 × 10 12 . Tested variants: water, CS (0.25 mg/mL), AuMNPs (1 mg/mL), Zn/CS NPs/Dox-AuMNPs (Dox = 6 µM) and AntiSar/Zn/CS NPs/Dox-AuMNPs (Dox = 6 µM). Inset photographs of each variant (centrifugation 2000 rpm, 5 min. to a completely clear solution). The pellet was diluted by PBS. The supernatant was spectrophotometrically analyzed, the absorbance spectra was obtained and the results were evaluated in 570 nm. Experiment was carried out using two replicates. ( B ) Toxicity evaluation of complexes using eukaryotic cells Saccharomyces cerevisiae . Characteristic growth curves for CS (0.25 mg/mL), AuMNPs (1 mg/mL), Zn/CS NPs/Dox-AuMNPs, AntiSar/Zn/CS NPs/Dox-AuMNPs and Dox (0.75 µM). ( C ) Average inhibition effect of samples: (0) S. cerevisiae , (1) AuMNPs (1 mg/mL), (2) CS (2 mg/mL), (3) Zn/CS NPs/Dox-AuMNPs (Dox 6 µM), (4) AntiSar/Zn/CS NPs/Dox-AuMNPs (Dox 6 µM), (5) Dox (0.25 µM), (6) Dox (0.5 µM), (7) Dox (1 µM). Data are expressed as an average of 12 repetitions. See to Materials and Methods Section for more information regarding experimental details.

    Article Snippet: S. cerevisiae (ATCC 9763) were obtained from the Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Brno, Czech Republic.

    Techniques: Activity Assay, Variant Assay, Centrifugation, Inhibition

    (a) ALDH zymogram: 1–6, Saccharomyces cerevisiae ALDH controls: 1, 0.1 U; 2, 0.25 U; 3, 0.5 U; 4, 0.75 U; 5, 1 U; 6, 3 U; 7, blank; 8, S. gordonii V2016wt. (b) ADH zymogram: 1–3, S. gordonii V2016wt, V2016Δ adhE and V288; 4 and

    Journal: Microbiology

    Article Title: Multiple alcohol dehydrogenases but no functional acetaldehyde dehydrogenase causing excessive acetaldehyde production from ethanol by oral streptococci

    doi: 10.1099/mic.0.066258-0

    Figure Lengend Snippet: (a) ALDH zymogram: 1–6, Saccharomyces cerevisiae ALDH controls: 1, 0.1 U; 2, 0.25 U; 3, 0.5 U; 4, 0.75 U; 5, 1 U; 6, 3 U; 7, blank; 8, S. gordonii V2016wt. (b) ADH zymogram: 1–3, S. gordonii V2016wt, V2016Δ adhE and V288; 4 and

    Article Snippet: As shown in , we tested S. gordonii V2016 with the ALDH of Saccharomyces cerevisiae (Sigma) as the positive control.

    Techniques:

    ) from 13 sequences, including, in addition to the sequences shown, ALKBH8 from Caenorhabditis elegans and Drosophila melanogaster , ALKBH8 and KIAA1456 from Gallus gallus and Xenopus tropicalis , and Trm9 from Arabidopsis thaliana . Arrows indicate a GXGXG motif expected to be critical for methyltransferase activity. Hs, Homo sapiens ; Mm, Mus musculus ; Sc, Saccharomyces cerevisiae ; Sp, Schizosaccharomyces pombe .

    Journal: Molecular and Cellular Biology

    Article Title: Mammalian ALKBH8 Possesses tRNA Methyltransferase Activity Required for the Biogenesis of Multiple Wobble Uridine Modifications Implicated in Translational Decoding ▿

    doi: 10.1128/MCB.01602-09

    Figure Lengend Snippet: ) from 13 sequences, including, in addition to the sequences shown, ALKBH8 from Caenorhabditis elegans and Drosophila melanogaster , ALKBH8 and KIAA1456 from Gallus gallus and Xenopus tropicalis , and Trm9 from Arabidopsis thaliana . Arrows indicate a GXGXG motif expected to be critical for methyltransferase activity. Hs, Homo sapiens ; Mm, Mus musculus ; Sc, Saccharomyces cerevisiae ; Sp, Schizosaccharomyces pombe .

    Article Snippet: Total tRNA isolated from mouse organs, Bos taurus liver (Novagene), E. coli (Roche), or Saccharomyces cerevisiae (Roche) was incubated with purified recombinant protein for 12 min at 37° in a 50-μl reaction mixture containing 1.33 μM s -adenosyl- l -[ methyl -3 H]methionine (GE Healthcare), 25 μM s -adenosyl- l -methionine (NEB), 50 mM Tris-HCl, pH 7.5, 25 mM KCl, 25 mM NH4 Ac, 0.5 mM MgCl2 , 0.1 mM EDTA, and 10 U RNasin Plus RNase inhibitor (Promega).

    Techniques: Activity Assay

    Upon expression on surface of HEK293 cells α X β 2 supports adhesive, migratory and phagocytic activities of the cells toward C. albicans . ( A ) HEK293/α X β 2 cell adhesion to C. albicans . A total of 5 × 10 5 HEK293/α X β 2 (▪) or mock-transfected (□) cells were added to wells of tissue culture plates containing germinated (hyphae) or nongerminated (yeast) C. albicans of WT (SC5314) or ΔPra1 (CAMB5-18) strains. The plates were incubated at 37°C for 1 h, then nonadherent cells were removed by washing, and the adherent cells were quantified using the CyQUANT Cell Proliferation Assay kit. For inhibition assays, before addition to the plate wells, the cells were preincubated with 10 μg/ml of the Abs: anti-α M I-domain (44a), anti-α M lectin domain (OKM1), anti-β 2 (IB4), anti-α X (3.9), and irrelevant anti–MHC-II (W6/32) or with 1 mM β-glucan, 1 mM mannan, or 5 μM recombinant NIF for 10 min at room temperature. ( B ) HEK293/α X β 2 cell migration to C. albicans . Cell migration was measured in Boyden chambers (Costar Transwell with 8-μm porosity in a 24-well format). A total of 10 5 HEK293/α X β 2 cells were added to the upper chamber, whereas lower chambers contained 10 6 germinated C. albicans cells of WT SC5314 strain (□) or 10 mM vitronectin (▪) in serum-free DMEM/F-12 medium. The inhibitors mAbs IB4, OKM1, 3.9 (see above), anti-α V (27217E6), and 1 mM β-glucan or echistatin were added with the cells to the upper chamber. Plates were incubated for 8 h in a humidified incubator at 37°C and 5% CO 2 . The migrated cells were counted using the CyQUANT Cell Proliferation kit. ( C ) Phagocytosis of C. albicans by HEK293/α X β 2 cells. A total of 10 5 germinated C. albicans SC5314 strain cells were incubated with 3 × 10 5 (1:3 ratio), 7 × 10 5 (1:7 ratio), or 1.1 × 10 6 (1:11 ratio) mock-transfected HEK293 cells (●) or HEK293/α X β 2 cells in the presence (▴) or absence (○) of anti-α X mAbs 3.9. Fungal survival was determined every 20 min by plating the sample aliquots onto SDA plates in serial dilutions. Results are presented as percentage (mean ± SE) of total cells (to which was assigned the value of 100%) and represent the results of three independent experiments, each in triplicate. * p

    Journal: The Journal of Immunology Author Choice

    Article Title: Integrin ?X?2 Is a Leukocyte Receptor for Candida albicans and Is Essential for Protection against Fungal Infections

    doi: 10.4049/jimmunol.1200524

    Figure Lengend Snippet: Upon expression on surface of HEK293 cells α X β 2 supports adhesive, migratory and phagocytic activities of the cells toward C. albicans . ( A ) HEK293/α X β 2 cell adhesion to C. albicans . A total of 5 × 10 5 HEK293/α X β 2 (▪) or mock-transfected (□) cells were added to wells of tissue culture plates containing germinated (hyphae) or nongerminated (yeast) C. albicans of WT (SC5314) or ΔPra1 (CAMB5-18) strains. The plates were incubated at 37°C for 1 h, then nonadherent cells were removed by washing, and the adherent cells were quantified using the CyQUANT Cell Proliferation Assay kit. For inhibition assays, before addition to the plate wells, the cells were preincubated with 10 μg/ml of the Abs: anti-α M I-domain (44a), anti-α M lectin domain (OKM1), anti-β 2 (IB4), anti-α X (3.9), and irrelevant anti–MHC-II (W6/32) or with 1 mM β-glucan, 1 mM mannan, or 5 μM recombinant NIF for 10 min at room temperature. ( B ) HEK293/α X β 2 cell migration to C. albicans . Cell migration was measured in Boyden chambers (Costar Transwell with 8-μm porosity in a 24-well format). A total of 10 5 HEK293/α X β 2 cells were added to the upper chamber, whereas lower chambers contained 10 6 germinated C. albicans cells of WT SC5314 strain (□) or 10 mM vitronectin (▪) in serum-free DMEM/F-12 medium. The inhibitors mAbs IB4, OKM1, 3.9 (see above), anti-α V (27217E6), and 1 mM β-glucan or echistatin were added with the cells to the upper chamber. Plates were incubated for 8 h in a humidified incubator at 37°C and 5% CO 2 . The migrated cells were counted using the CyQUANT Cell Proliferation kit. ( C ) Phagocytosis of C. albicans by HEK293/α X β 2 cells. A total of 10 5 germinated C. albicans SC5314 strain cells were incubated with 3 × 10 5 (1:3 ratio), 7 × 10 5 (1:7 ratio), or 1.1 × 10 6 (1:11 ratio) mock-transfected HEK293 cells (●) or HEK293/α X β 2 cells in the presence (▴) or absence (○) of anti-α X mAbs 3.9. Fungal survival was determined every 20 min by plating the sample aliquots onto SDA plates in serial dilutions. Results are presented as percentage (mean ± SE) of total cells (to which was assigned the value of 100%) and represent the results of three independent experiments, each in triplicate. * p

    Article Snippet: Baker yeast β-glucan, mannan, and echistatin ( , ) were purchased from Sigma-Aldrich (St. Louis, MO).

    Techniques: Expressing, Transfection, Incubation, CyQUANT Assay, Proliferation Assay, Inhibition, Recombinant, Migration

    Biosynthesis of precursor oligosaccharides for enzymatic remodeling. MALDI-TOF MS analysis (top panels) and 600-MHz 1 H NMR characterization (bottom panels) corresponding to: ( a ) Man 5 GlcNAc 2 glycan synthesized by enzymatic deglycosylation of S. cerevisiae oligosaccharides; and ( b ) the Man 3 GlcNAc 2 glycan synthesized by glycoengineered E. coli cells.

    Journal: Scientific Reports

    Article Title: A library of chemically defined human N-glycans synthesized from microbial oligosaccharide precursors

    doi: 10.1038/s41598-017-15891-8

    Figure Lengend Snippet: Biosynthesis of precursor oligosaccharides for enzymatic remodeling. MALDI-TOF MS analysis (top panels) and 600-MHz 1 H NMR characterization (bottom panels) corresponding to: ( a ) Man 5 GlcNAc 2 glycan synthesized by enzymatic deglycosylation of S. cerevisiae oligosaccharides; and ( b ) the Man 3 GlcNAc 2 glycan synthesized by glycoengineered E. coli cells.

    Article Snippet: Synthesis of Man5 GlcNAc2 precursor 5 g of S. cerevisiae invertase (Sigma-Aldrich) was denatured in the presence of sodium dodecyl sulfate and NP-40 as described in the New England Biolabs protocol for PNGase F treatment.

    Techniques: Mass Spectrometry, Nuclear Magnetic Resonance, Synthesized

    Generality of ssRNA detection. Hairpin-guides were designed to target two additional physiologically relevant transcribed RNAs: (A) human homologue of murine double minute 2 (hDM2) and (B) human epidermal growth factor receptor 2 (HER2). The sequences to which complementary guides were designed are indicated with targeted positions shown in parentheses. The designed hairpin-guides were annealed to their intended targets, followed by incubation of 1 nM annealed target with the CLuciferase-E2C and Aart-NLuciferase biosensors. (C) The hDM2, HER2, and VEGF hairpin-guides were each annealed in the presence of 1 nM hDM2, HER2, and VEGF RNA targets, followed by incubation with CLuciferase-E2C and Aart-NLuciferase. ALU, arbitrary luminescent units.

    Journal: Journal of the American Chemical Society

    Article Title: Toward a General Approach for RNA-Templated Hierarchical Assembly of Split-Proteins

    doi: 10.1021/ja104395b

    Figure Lengend Snippet: Generality of ssRNA detection. Hairpin-guides were designed to target two additional physiologically relevant transcribed RNAs: (A) human homologue of murine double minute 2 (hDM2) and (B) human epidermal growth factor receptor 2 (HER2). The sequences to which complementary guides were designed are indicated with targeted positions shown in parentheses. The designed hairpin-guides were annealed to their intended targets, followed by incubation of 1 nM annealed target with the CLuciferase-E2C and Aart-NLuciferase biosensors. (C) The hDM2, HER2, and VEGF hairpin-guides were each annealed in the presence of 1 nM hDM2, HER2, and VEGF RNA targets, followed by incubation with CLuciferase-E2C and Aart-NLuciferase. ALU, arbitrary luminescent units.

    Article Snippet: For detection of VEGF in the presence of yeast RNA (Ribonucleic acid mixture from baker's yeast ( S. cerevisiae ) containing mRNA, tRNA, and rRNA, Sigma R6750), translations were performed followed by addition of 500 pM (5.0 ng) annealed VEGF RNA, 500 pM annealed VEGF + 2000-fold mass excess (10 μg) yeast RNA, yeast RNA + HP-guides, or HP-guides only.

    Techniques: Incubation

    VEGF ssRNA-templated reassembly of split-luciferase. (A) CLuciferase-E2C and Aart-NLuciferase biosensors were reassembled in the presence of a 295-nucleotide (nt) VEGF transcript (1 nM) annealed to designed hairpin-guides with guide lengths of 19-nt. (B) CLuciferase-E2C and Aart-NLuciferase were incubated for 5, 10, 20, 30, 45, or 60 minutes with the VEGF target (1 nM) annealed to hairpin-guides with guide lengths of 19-nt, followed by luminescence readings. (C) CLuciferase-E2C and Aart-NLuciferase were reassembled in the presence of decreasing concentrations, 500, 250, 125, 62.5, 10 pM, of the VEGF target annealed to hairpin-guides with guide lengths of 19-nt. Upon subtraction of the luminescence contributed by the corresponding No target samples, a linear response to change in RNA concentration is observed (inset). (D) The length of the complementary guides (red) was varied (15, 17, 19, 21, 23, or 25-nt) to identify the optimal length. (E) Pairs of the hairpin-guides described in (C) were annealed to the VEGF target (1 nM), and incubated with CLuciferase-E2C and Aart-NLuciferase, followed by luminescence readings. The length of guides is presented as the combined length of complementarity of a pair of guides. (F) The annealed VEGF target (500 pM = 5.0 ng) was added to ~2000-fold mass excess (10 μg) of yeast RNA and was incubated in the presence of CLuciferase-E2C and Aart-NLuciferase, followed by luminescence readings. ALU, arbitrary luminescent units. CFluc, C-terminal fragment of luciferase and NFluc, N-terminal fragment of luciferase.

    Journal: Journal of the American Chemical Society

    Article Title: Toward a General Approach for RNA-Templated Hierarchical Assembly of Split-Proteins

    doi: 10.1021/ja104395b

    Figure Lengend Snippet: VEGF ssRNA-templated reassembly of split-luciferase. (A) CLuciferase-E2C and Aart-NLuciferase biosensors were reassembled in the presence of a 295-nucleotide (nt) VEGF transcript (1 nM) annealed to designed hairpin-guides with guide lengths of 19-nt. (B) CLuciferase-E2C and Aart-NLuciferase were incubated for 5, 10, 20, 30, 45, or 60 minutes with the VEGF target (1 nM) annealed to hairpin-guides with guide lengths of 19-nt, followed by luminescence readings. (C) CLuciferase-E2C and Aart-NLuciferase were reassembled in the presence of decreasing concentrations, 500, 250, 125, 62.5, 10 pM, of the VEGF target annealed to hairpin-guides with guide lengths of 19-nt. Upon subtraction of the luminescence contributed by the corresponding No target samples, a linear response to change in RNA concentration is observed (inset). (D) The length of the complementary guides (red) was varied (15, 17, 19, 21, 23, or 25-nt) to identify the optimal length. (E) Pairs of the hairpin-guides described in (C) were annealed to the VEGF target (1 nM), and incubated with CLuciferase-E2C and Aart-NLuciferase, followed by luminescence readings. The length of guides is presented as the combined length of complementarity of a pair of guides. (F) The annealed VEGF target (500 pM = 5.0 ng) was added to ~2000-fold mass excess (10 μg) of yeast RNA and was incubated in the presence of CLuciferase-E2C and Aart-NLuciferase, followed by luminescence readings. ALU, arbitrary luminescent units. CFluc, C-terminal fragment of luciferase and NFluc, N-terminal fragment of luciferase.

    Article Snippet: For detection of VEGF in the presence of yeast RNA (Ribonucleic acid mixture from baker's yeast ( S. cerevisiae ) containing mRNA, tRNA, and rRNA, Sigma R6750), translations were performed followed by addition of 500 pM (5.0 ng) annealed VEGF RNA, 500 pM annealed VEGF + 2000-fold mass excess (10 μg) yeast RNA, yeast RNA + HP-guides, or HP-guides only.

    Techniques: Luciferase, Incubation, Concentration Assay