Journal: Biotechnology for Biofuels

Article Title: Improvement of sabinene tolerance of Escherichia coli using adaptive laboratory evolution and omics technologies

doi: 10.1186/s13068-020-01715-x

Figure Lengend Snippet: Electron micrographs of different strains grown with or without sabinene treatment. a Cell morphology of BL21(DE3) grown without sabinene; b cell morphology of BL21(DE3) grown with 0.6 g/L sabinene; c cell morphology of XYF(DE3) grown with 0.6 g/L sabinene; d – g cell morphology of WUT1, WUT2, WUT3, and WUT4 grown with 0.6 g/L sabinene, respectively. h – n (magnification of ×80,000) are enlarged images of a – g (magnification of ×30,000), respectively

Article Snippet: The peak area was converted into sabinene concentration in comparison with a standard curve plotted with a set of known concentrations of commercial sabinene bought from Sigma-Aldrich.

Techniques:

Journal: Biotechnology for Biofuels

Article Title: Improvement of sabinene tolerance of Escherichia coli using adaptive laboratory evolution and omics technologies

doi: 10.1186/s13068-020-01715-x

Figure Lengend Snippet: Growth curves of reverse-engineered strains under sabinene stress. Squares with solid lines indicate strain WUT1, expressing empty plasmid pACYCDuet-1. Circles with dashed lines indicate strain WUT2, overexpressing gene ybcK . Triangles with dashed lines indicate strain WUT3, overexpressing gene scpA . Inverted triangles with dashed lines indicate strain WUT4, overexpressing gene ygiZ . The experiments were carried out in triplicates. Error bars represent the standard deviation from the mean

Article Snippet: The peak area was converted into sabinene concentration in comparison with a standard curve plotted with a set of known concentrations of commercial sabinene bought from Sigma-Aldrich.

Techniques: Expressing, Plasmid Preparation, Standard Deviation

Journal: Biotechnology for Biofuels

Article Title: Improvement of sabinene tolerance of Escherichia coli using adaptive laboratory evolution and omics technologies

doi: 10.1186/s13068-020-01715-x

Figure Lengend Snippet: Synthetic pathway of sabinene. a Flow chart of sabinene synthetic pathway. MEP pathway intermediates: DXP, 1-deoxy- d -xylulose 5-phosphate; MEP, 2-C-methyl- d -erythritol-4-phosphate; CDP-ME, 4-(cytidine-5′-diphospho)-2-C-methyl- d -erythritol; CDP-MEP, 2-phospho-4-(cytidine-5′-di-phospho)-2-C-methyl- d -erythritol; ME-cPP, 2-C-methyl- d -erythritol 2,4-cyclodiphosphate; HMBPP, 4-hydroxy-3-methylbut-2-enyl diphosphate; IPP, isopentenyl diphosphate; DMAPP, dimethylallyl diphosphate. Enzymes in MEP pathway: DXS, DXP synthase; DXR, DXP reductoisomerase; MCT, CDP-ME synthase; CMK, CDP-ME kinase; MDS, ME-cPP synthase; HDS, HMBPP synthase: HDR, HMBPP reductase; IDI, IPP isomerase. MVA pathway intermediates: Acyl-CoA, acetyl-CoA; AA-CoA, acetoacetyl-CoA; HMG-CoA, hydroxymethylglutaryl-CoA; Mev-P, mevalonate-5-phosphate; Mev-PP, mevalonate pyrophosphate; IPP, isopentenyl pyrophosphate; DMAPP, dimethylallyl pyrophosphate. Enzymes in MVA pathway: MvaE, acetyl-CoA acetyltransferase/HMG-CoA reductase; MvaS, HMG-CoA synthase; ERG12, mevalonate kinase; ERG8, phosphomevalonate kinase; ERG19, mevalonate pyrophosphate decarboxylase; IDI, IPP isomerase. GPPS2, geranyl diphosphate from Abies grandis ; SabS1, sabinene synthase from Salvia pomifera . b Schematic diagram of plasmids pHB7 and pTrcLower

Article Snippet: The peak area was converted into sabinene concentration in comparison with a standard curve plotted with a set of known concentrations of commercial sabinene bought from Sigma-Aldrich.

Techniques:

Journal: Biotechnology for Biofuels

Article Title: Improvement of sabinene tolerance of Escherichia coli using adaptive laboratory evolution and omics technologies

doi: 10.1186/s13068-020-01715-x

Figure Lengend Snippet: Schematic diagram of the ALE process. Gradually increasing concentrations of sabinene were used as the driving force for the evolution

Article Snippet: The peak area was converted into sabinene concentration in comparison with a standard curve plotted with a set of known concentrations of commercial sabinene bought from Sigma-Aldrich.

Techniques:

Journal: Biotechnology for Biofuels

Article Title: Improvement of sabinene tolerance of Escherichia coli using adaptive laboratory evolution and omics technologies

doi: 10.1186/s13068-020-01715-x

Figure Lengend Snippet: Sabinene production of BL21(DE3) and XYF(DE3). a Sabinene production using glucose as a carbon source. b Sabinene production using glycerol as a carbon source. HB4, BL21(DE3) harboring pHB7 and pTrcLower. XYFHB7, XYF(DE3) harboring pHB7 and pTrcLower. The experiments were performed in triplicates. Error bars represent the standard deviation from the mean

Article Snippet: The peak area was converted into sabinene concentration in comparison with a standard curve plotted with a set of known concentrations of commercial sabinene bought from Sigma-Aldrich.

Techniques: Standard Deviation

Journal: Biotechnology for Biofuels

Article Title: Improvement of sabinene tolerance of Escherichia coli using adaptive laboratory evolution and omics technologies

doi: 10.1186/s13068-020-01715-x

Figure Lengend Snippet: Growth curves of BL21(DE3) and XYF(DE3) under sabinene stress. Squares represent the growth of BL21(DE3) without sabinene stress. Circles and triangles represent the growth of BL21(DE3) and XYF(DE3) with 0.6 g/L sabinene. After 2.5 h of cultivation, sabinene was added to a final concentration of 0.6 g/L. Cell density was monitored for 24 h. The experiments were carried out in triplicates. Error bars represent the standard deviation from the mean

Article Snippet: The peak area was converted into sabinene concentration in comparison with a standard curve plotted with a set of known concentrations of commercial sabinene bought from Sigma-Aldrich.

Techniques: Concentration Assay, Standard Deviation

Journal: Phytochemistry

Article Title: Natural product studies of U.S. endangered plants: Volatile components of Lindera melissifolia (Lauraceae) repel mosquitoes and ticks

doi: 10.1016/j.phytochem.2012.05.001

Figure Lengend Snippet: 1 H NMR spectrum of pondberry essential oil and identified major components of the oil (CDCl 3 , 400 MHz). (i) ( E )-β-ocimene, (ii) sabinene, (iii) α-phellandrene, (iv) limonene, (v) p -cymene, (vi) myrcene.

Article Snippet: N , N -Diethyl- 3-methylbenzamide (DEET) and trans -sabinene hydrate used for the tick and mosquito assays were purchased from Sigma–Aldrich, Inc., St. Louis, MO.

Techniques: Nuclear Magnetic Resonance