Journal: Biotechnology for Biofuels
Article Title: Improvement of sabinene tolerance of Escherichia coli using adaptive laboratory evolution and omics technologies
Figure Lengend Snippet: Synthetic pathway of sabinene. a Flow chart of sabinene synthetic pathway. MEP pathway intermediates: DXP, 1-deoxy- d -xylulose 5-phosphate; MEP, 2-C-methyl- d -erythritol-4-phosphate; CDP-ME, 4-(cytidine-5′-diphospho)-2-C-methyl- d -erythritol; CDP-MEP, 2-phospho-4-(cytidine-5′-di-phospho)-2-C-methyl- d -erythritol; ME-cPP, 2-C-methyl- d -erythritol 2,4-cyclodiphosphate; HMBPP, 4-hydroxy-3-methylbut-2-enyl diphosphate; IPP, isopentenyl diphosphate; DMAPP, dimethylallyl diphosphate. Enzymes in MEP pathway: DXS, DXP synthase; DXR, DXP reductoisomerase; MCT, CDP-ME synthase; CMK, CDP-ME kinase; MDS, ME-cPP synthase; HDS, HMBPP synthase: HDR, HMBPP reductase; IDI, IPP isomerase. MVA pathway intermediates: Acyl-CoA, acetyl-CoA; AA-CoA, acetoacetyl-CoA; HMG-CoA, hydroxymethylglutaryl-CoA; Mev-P, mevalonate-5-phosphate; Mev-PP, mevalonate pyrophosphate; IPP, isopentenyl pyrophosphate; DMAPP, dimethylallyl pyrophosphate. Enzymes in MVA pathway: MvaE, acetyl-CoA acetyltransferase/HMG-CoA reductase; MvaS, HMG-CoA synthase; ERG12, mevalonate kinase; ERG8, phosphomevalonate kinase; ERG19, mevalonate pyrophosphate decarboxylase; IDI, IPP isomerase. GPPS2, geranyl diphosphate from Abies grandis ; SabS1, sabinene synthase from Salvia pomifera . b Schematic diagram of plasmids pHB7 and pTrcLower
Article Snippet: The peak area was converted into sabinene concentration in comparison with a standard curve plotted with a set of known concentrations of commercial sabinene bought from Sigma-Aldrich.